Restriction Fragments for RPA49/YNL248C



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The currently selected gene/sequence is :

RPA49/YNL248C on chromosome XIV from coordinates 182608 to 181361.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2625.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 15 + BsiYI* CCNNNNNNNGG 11 8 . . 13 16 + SetI ASST 16 12 . . 22 26 + Hpy188I TCNGA 24 23 . . 29 32 + TaqI TCGA 29 31 . . 33 36 + TspEI AATT 32 36 . . 36 47 + BdaI TGANNNNNNTCA 25 23 59 57 37 46 + XmnI GAANNNNTTC 41 41 . . 45 50 + Hpy178III* TCNNGA 46 48 . . 36 47 - BdaI TGANNNNNNTCA 59 57 25 23 49 59 + Hin4I GAYNNNNNVTC 40 35 72 67 49 52 + DpnI GATC 50 50 . . 49 52 + BstKTI GATC 51 49 . . 49 52 + MboI GATC 48 52 . . 56 59 + MnlI CCTC 66 65 . . 65 70 + DsaI* CCRYGG 65 69 . . 65 70 + SecI* CCNNGG 65 69 . . 64 68 - BceAI ACGGC 49 51 . . 77 81 - MboII GAAGA 69 68 . . 85 88 + CviJI RGCY 86 86 . . 92 97 + SduI GDGCHC 96 92 . . 92 97 + HgiAI* GWGCWC 96 92 . . 89 93 - TspGWI ACGGA 79 77 . . 99 105 + TspRI CASTGNN 105 96 . . 101 105 + Tsp45I GTSAC 100 105 . . 101 105 + MaeIII GTNAC 100 105 . . 98 102 - BsrI ACTGG 98 96 . . 97 102 - BfiI ACTGGG 92 91 . . 109 112 + TaiI ACGT 112 108 . . 109 112 + SetI ASST 112 108 . . 109 112 + MaeII ACGT 109 111 . . 113 116 + TaqI TCGA 113 115 . . 136 140 + Hpy188I TCNGA 138 137 . . 148 153 + EcoRI GAATTC 148 152 . . 148 153 + ApoI RAATTY 148 152 . . 149 152 + TspEI AATT 148 152 . . 161 165 + Tsp4CI* ACNGT 163 162 . . 163 167 + HphI GGTGA 175 174 . . 178 181 + MaeI CTAG 178 180 . . 174 178 - BsmAI GTCTC 168 172 . . 189 192 + SetI ASST 192 188 . . 187 191 - Hin4II* CCTTC 181 180 . . 199 203 + TfiI GAWTC 199 202 . . 199 203 + HinfI GANTC 199 202 . . 202 206 - MboII GAAGA 194 193 . . 205 209 - MboII GAAGA 197 196 . . 212 217 + HindIII AAGCTT 212 216 . . 213 216 + SetI ASST 216 212 . . 213 216 + CviJI RGCY 214 214 . . 213 216 + AluI AGCT 214 214 . . 216 221 + AsuII TTCGAA 217 219 . . 217 220 + TaqI TCGA 217 219 . . 222 226 - BsrI ACTGG 222 220 . . 234 238 + BmgT120I GGNCC 235 236 . . 234 238 + AsuI* GGNCC 234 237 . . 235 238 + CviJI RGCY 236 236 . . 235 238 + HaeIII GGCC 236 236 . . 242 245 + MseI TTAA 242 244 . . 246 251 + Hpy178III* TCNNGA 247 249 . . 276 279 + CviJI RGCY 277 277 . . 276 281 + NlaIV GGNNCC 278 278 . . 283 286 + Csp6I GTAC 283 285 . . 283 286 + RsaI GTAC 284 284 . . 301 306 + BciVI GTATCC 312 311 . . 318 322 + TfiI GAWTC 318 321 . . 318 322 + HinfI GANTC 318 321 . . 322 326 + DdeI CTNAG 322 325 . . 327 332 + NlaIV GGNNCC 329 329 . . 328 332 + BmgT120I GGNCC 329 330 . . 328 332 + AvaII GGWCC 328 331 . . 328 332 + AsuI* GGNCC 328 331 . . 348 358 + BplI GAGNNNNNCTC 339 334 371 366 348 358 - BplI GAGNNNNNCTC 371 366 339 334 361 365 + BccI CCATC 369 370 . . 385 389 + HphI GGTGA 397 396 . . 383 388 - TaqII CACCCA 373 371 . . 398 401 + Csp6I GTAC 398 400 . . 398 401 + RsaI GTAC 399 399 . . 408 411 + CviJI RGCY 409 409 . . 409 419 + MwoI GCNNNNNNNGC 415 412 . . 417 420 + SetI ASST 420 416 . . 417 420 + CviJI RGCY 418 418 . . 417 420 + AluI AGCT 418 418 . . 423 426 + CviRI* TGCA 424 424 . . 426 431 + XhoII RGATCY 426 430 . . 426 431 + BglII AGATCT 426 430 . . 427 430 + DpnI GATC 428 428 . . 427 430 + BstKTI GATC 429 427 . . 427 430 + MboI GATC 426 430 . . 429 434 + XbaI TCTAGA 429 433 . . 429 434 + Hpy178III* TCNNGA 430 432 . . 430 433 + MaeI CTAG 430 432 . . 440 444 + Tsp4CI* ACNGT 442 441 . . 448 452 + HinfI GANTC 448 451 . . 451 455 + Hpy188I TCNGA 453 452 . . 448 452 - PleI GAGTC 442 443 . . 448 452 - MlyI GAGTC 442 442 . . 459 464 + Hpy166II GTNNAC 461 461 . . 459 464 + HindII GTYRAC 461 461 . . 466 470 + TfiI GAWTC 466 469 . . 466 470 + HinfI GANTC 466 469 . . 487 491 + TfiI GAWTC 487 490 . . 487 491 + HinfI GANTC 487 490 . . 490 494 - TspGWI ACGGA 480 478 . . 501 504 + CviRI* TGCA 502 502 . . 502 506 + SfaNI GCATC 511 515 . . 506 510 + DdeI CTNAG 506 509 . . 521 526 + BmeT110I CYCGRG 522 524 . . 521 526 + AvaI CYCGRG 521 525 . . 524 529 + SduI GDGCHC 528 524 . . 524 529 + HgiJII* GRGCYC 528 524 . . 525 528 + CviJI RGCY 526 526 . . 529 534 + MfeI CAATTG 529 533 . . 530 533 + TspEI AATT 529 533 . . 534 538 + FokI GGATG 547 551 . . 534 538 + BseGI GGATG 540 538 . . 536 540 + TspDTI ATGAA 551 549 . . 540 543 + TspEI AATT 539 543 . . 547 552 + MmeI TCCRAC 572 570 . . 558 561 + SetI ASST 561 557 . . 569 574 + TsoI TARCCA 585 583 . . 570 573 + CviJI RGCY 571 571 . . 577 582 + ClaI ATCGAT 578 580 . . 578 581 + TaqI TCGA 578 580 . . 580 584 - SfaNI GCATC 570 574 . . 585 591 + TspRI CASTGNN 591 582 . . 599 604 + ApoI RAATTY 599 603 . . 600 603 + TspEI AATT 599 603 . . 608 613 + MfeI CAATTG 608 612 . . 609 612 + TspEI AATT 608 612 . . 630 634 - Hin4II* CCTTC 624 623 . . 635 638 + TspEI AATT 634 638 . . 639 643 + Tsp4CI* ACNGT 641 640 . . 649 652 + FnuDII* CGCG 650 650 . . 654 658 - TspDTI ATGAA 644 642 . . 660 663 + TspEI AATT 659 663 . . 663 667 + Hpy188I TCNGA 665 664 . . 673 676 - AciI CCGC 673 675 . . 695 698 + TspEI AATT 694 698 . . 690 695 - MmeI TCCRAC 671 669 . . 715 718 + TspEI AATT 714 718 . . 719 730 + BstXI CCANNNNNNTGG 726 722 . . 737 740 + TspEI AATT 736 740 . . 742 746 + HinfI GANTC 742 745 . . 742 746 - PleI GAGTC 736 737 . . 742 746 - MlyI GAGTC 736 736 . . 756 759 + SetI ASST 759 755 . . 757 761 + Hin4II* CCTTC 767 766 . . 773 776 + TspEI AATT 772 776 . . 779 782 + SetI ASST 782 778 . . 779 782 + CviJI RGCY 780 780 . . 779 782 + AluI AGCT 780 780 . . 804 808 + BsrI ACTGG 809 807 . . 804 809 + BfiI ACTGGG 814 813 . . 811 816 + Hpy166II GTNNAC 813 813 . . 811 816 + BssNAI GTATAC 813 813 . . 811 816 + AccI GTMKAC 812 814 . . 824 828 + HgaI GACGC 833 838 . . 825 830 + MluI ACGCGT 825 829 . . 825 830 + AflIII ACRYGT 825 829 . . 826 829 + FnuDII* CGCG 827 827 . . 856 859 + SetI ASST 859 855 . . 863 867 + Tsp4CI* ACNGT 865 864 . . 868 871 + MnlI CCTC 878 877 . . 870 875 + Hpy178III* TCNNGA 871 873 . . 886 890 - BccI CCATC 880 881 . . 894 899 + SmlI CTYRAG 894 898 . . 894 899 + AflII CTTAAG 894 898 . . 895 898 + MseI TTAA 895 897 . . 907 911 + Tsp4CI* ACNGT 909 908 . . 917 920 + CviJI RGCY 918 918 . . 919 923 + BssKI CCNGG 918 923 . . 919 923 + BseBI CCWGG 920 921 . . 919 923 + ScrFI CCNGG 920 921 . . 919 923 + EcoRII CCWGG 918 923 . . 924 928 + Tsp4CI* ACNGT 926 925 . . 932 936 + MboII GAAGA 944 943 . . 935 938 + DpnI GATC 936 936 . . 935 938 + BstKTI GATC 937 935 . . 935 938 + MboI GATC 934 938 . . 942 946 + BinI* GGATC 950 951 . . 943 946 + DpnI GATC 944 944 . . 943 946 + BstKTI GATC 945 943 . . 943 946 + MboI GATC 942 946 . . 955 959 - TspDTI ATGAA 945 943 . . 971 975 + TspDTI ATGAA 986 984 . . 973 977 + MboII GAAGA 985 984 . . 1009 1014 + EcoT22I ATGCAT 1013 1009 . . 1010 1013 + CviRI* TGCA 1011 1011 . . 1024 1028 - TspDTI ATGAA 1014 1012 . . 1043 1049 + Bpu10I CCTNAGC 1044 1047 . . 1044 1048 + DdeI CTNAG 1044 1047 . . 1055 1058 + TspEI AATT 1054 1058 . . 1068 1071 + SetI ASST 1071 1067 . . 1069 1073 + Hin4II* CCTTC 1079 1078 . . 1096 1099 + Csp6I GTAC 1096 1098 . . 1096 1099 + RsaI GTAC 1097 1097 . . 1116 1119 + SetI ASST 1119 1115 . . 1117 1122 + NlaIV GGNNCC 1119 1119 . . 1117 1122 + HgiCI* GGYRCC 1117 1121 . . 1120 1130 + MwoI GCNNNNNNNGC 1126 1123 . . 1120 1130 + BglI GCCNNNNNGGC 1126 1123 . . 1123 1127 + Tsp4CI* ACNGT 1125 1124 . . 1124 1130 + TspRI CASTGNN 1130 1121 . . 1128 1131 + CviJI RGCY 1129 1129 . . 1130 1135 + SmlI CTYRAG 1130 1134 . . 1134 1137 + SetI ASST 1137 1133 . . 1134 1137 + CviJI RGCY 1135 1135 . . 1134 1137 + AluI AGCT 1135 1135 . . 1130 1135 - Bce83I* CTTGAG 1115 1113 . . 1136 1140 + DdeI CTNAG 1136 1139 . . 1140 1143 + CviJI RGCY 1141 1141 . . 1136 1140 - BspCNI CTCAG 1128 1126 . . 1136 1140 - BseMII CTCAG 1127 1125 . . 1138 1141 - MnlI CCTC 1131 1130 . . 1135 1141 - Bpu10I CCTNAGC 1136 1139 . . 1135 1141 - BbvCI CCTCAGC 1136 1139 . . 1148 1153 - BsmI GAATGC 1148 1146 . . 1159 1164 + TatI WGTACW 1159 1163 . . 1159 1164 + ScaI AGTACT 1161 1161 . . 1160 1163 + Csp6I GTAC 1160 1162 . . 1160 1163 + RsaI GTAC 1161 1161 . . 1163 1168 + PstI CTGCAG 1167 1163 . . 1163 1168 + SfeI* CTRYAG 1163 1167 . . 1164 1167 + CviRI* TGCA 1165 1165 . . 1165 1169 + BlsI GCNGC 1167 1166 . . 1165 1169 + BisI GCNGC 1166 1167 . . 1165 1169 + BbvI GCAGC 1177 1181 . . 1165 1169 + TseI GCWGC 1165 1168 . . 1167 1170 + SetI ASST 1170 1166 . . 1167 1170 + CviJI RGCY 1168 1168 . . 1167 1170 + AluI AGCT 1168 1168 . . 1160 1172 - BarI GAAGNNNNNNTAC 1184 1179 1152 1147 1173 1178 + PsiI TTATAA 1175 1175 . . 1179 1182 + TspEI AATT 1178 1182 . . 1189 1193 + TspDTI ATGAA 1204 1202 . . 1202 1205 + MseI TTAA 1202 1204 . . 1205 1208 + SetI ASST 1208 1204 . . 1205 1208 + CviJI RGCY 1206 1206 . . 1205 1208 + AluI AGCT 1206 1206 . . 1209 1212 + SetI ASST 1212 1208 . . 1223 1227 + MboII GAAGA 1235 1234 . . 1223 1228 - Ksp632I* CTCTTC 1218 1221 . . 1226 1229 - MnlI CCTC 1219 1218 . . 1229 1233 + MboII GAAGA 1241 1240 . . 1233 1236 + SetI ASST 1236 1232 . . 1229 1234 - Ksp632I* CTCTTC 1224 1227 . . 1234 1238 + BmgT120I GGNCC 1235 1236 . . 1234 1238 + AvaII GGWCC 1234 1237 . . 1234 1238 + AsuI* GGNCC 1234 1237 . . 1232 1235 - MnlI CCTC 1225 1224 . . 1242 1245 + TaiI ACGT 1245 1241 . . 1242 1245 + SetI ASST 1245 1241 . . 1242 1245 + MaeII ACGT 1242 1244 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 436 812 # # AciI: # [CCGC] # 575 673 # # AflII: # [CTTAAG] # 354 894 # # AflIII: # [ACRYGT] # 423 825 # # AluI: # [AGCT] # 33 38 42 204 214 355 # 362 # # ApoI: # [RAATTY] # 148 451 649 # # AsuI*: # [GGNCC] # 14 94 234 906 # # AsuII: # [TTCGAA] # 217 1031 # # AvaI: # [CYCGRG] # 521 727 # # AvaII: # [GGWCC] # 14 328 906 # # BarI: # [GAAGNNNNNNTAC] # 32 64 1152 # # BbvCI: # [CCTCAGC] # 112 1136 # # BbvI: # [GCAGC] # 71 1177 # # BccI: # [CCATC] # 368 369 511 # # Bce83I*: # [CTTGAG] # 133 1115 # # BceAI: # [ACGGC] # 49 1199 # # BciVI: # [GTATCC] # 312 936 # # BdaI: # [TGANNNNNNTCA] # 0 0 25 34 1189 # # BfiI: # [ACTGGG] # 92 434 722 # # BglI: # [GCCNNNNNGGC] # 122 1126 # # BglII: # [AGATCT] # 426 822 # # BinI*: # [GGATC] # 298 950 # # BisI: # [GCNGC] # 82 1166 # # BlsI: # [GCNGC] # 81 1167 # # BmeT110I: # [CYCGRG] # 522 726 # # BmgT120I: # [GGNCC] # 13 94 235 906 # # BplI: # [GAGNNNNNCTC] # 0 0 32 339 877 # # Bpu10I: # [CCTNAGC] # 92 112 1044 # # BseBI: # [CCWGG] # 328 920 # # BseGI: # [GGATG] # 540 708 # # BseMII: # [CTCAG] # 121 1127 # # BsiYI*: # [CCNNNNNNNGG] # 11 1237 # # BsmAI: # [GTCTC] # 168 1080 # # BsmI: # [GAATGC] # 100 1148 # # BspCNI: # [CTCAG] # 120 1128 # # BsrI: # [ACTGG] # 98 124 439 587 # # BssKI: # [CCNGG] # 330 918 # # BssNAI: # [GTATAC] # 435 813 # # BstKTI: # [GATC] # 8 51 303 378 508 # # BstXI: # [CCANNNNNNTGG] # 522 726 # # ClaI: # [ATCGAT] # 578 670 # # Csp6I: # [GTAC] # 64 88 115 283 698 # # CviJI: # [RGCY] # 6 6 9 22 27 38 # 41 42 45 86 108 128 # 132 138 209 211 # # CviRI*: # [TGCA] # 78 83 154 424 509 # # DdeI: # [CTNAG] # 92 112 184 322 538 # # DpnI: # [GATC] # 8 50 304 378 508 # # DsaI*: # [CCRYGG] # 65 1183 # # EcoRI: # [GAATTC] # 148 1100 # # EcoRII: # [CCWGG] # 330 918 # # EcoT22I: # [ATGCAT] # 235 1013 # # FnuDII*: # [CGCG] # 177 421 650 # # FokI: # [GGATG] # 547 701 # # HaeIII: # [GGCC] # 236 1012 # # HgaI: # [GACGC] # 415 833 # # HgiAI*: # [GWGCWC] # 96 1152 # # HgiCI*: # [GGYRCC] # 131 1117 # # HgiJII*: # [GRGCYC] # 528 720 # # Hin4I: # [GAYNNNNNVTC] # 32 40 1176 # # Hin4II*: # [CCTTC] # 143 169 181 312 443 # # HindII: # [GTYRAC] # 461 787 # # HindIII: # [AAGCTT] # 212 1036 # # HinfI: # [GANTC] # 18 21 119 130 199 255 # 506 # # HphI: # [GGTGA] # 175 222 851 # # Hpy166II: # [GTNNAC] # 352 435 461 # # Hpy178III*: # [TCNNGA] # 46 183 201 377 441 # # Hpy188I: # [TCNGA] # 24 114 212 315 583 # # Ksp632I*: # [CTCTTC] # 6 24 1218 # # MaeI: # [CTAG] # 178 252 818 # # MaeII: # [ACGT] # 6 109 1133 # # MaeIII: # [GTNAC] # 100 1148 # # MboI: # [GATC] # 8 48 306 378 508 # # MboII: # [GAAGA] # 3 6 7 41 69 125 # 250 747 # # MfeI: # [CAATTG] # 79 529 640 # # MluI: # [ACGCGT] # 423 825 # # MlyI: # [GAGTC] # 294 442 512 # # MmeI: # [TCCRAC] # 99 572 577 # # MnlI: # [CCTC] # 6 23 66 88 253 812 # # MseI: # [TTAA] # 46 242 307 653 # # MwoI: # [GCNNNNNNNGC] # 122 415 711 # # NlaIV: # [GGNNCC] # 51 129 278 790 # # PleI: # [GAGTC] # 294 442 512 # # PsiI: # [TTATAA] # 73 1175 # # PstI: # [CTGCAG] # 81 1167 # # RsaI: # [GTAC] # 64 87 115 284 698 # # ScaI: # [AGTACT] # 87 1161 # # ScrFI: # [CCNGG] # 328 920 # # SduI: # [GDGCHC] # 96 432 720 # # SecI*: # [CCNNGG] # 65 1183 # # SetI: # [ASST] # 3 4 9 16 18 23 # 24 24 33 38 48 77 # 80 96 141 198 204 212 # # SfaNI: # [GCATC] # 59 511 678 # # SfeI*: # [CTRYAG] # 85 1163 # # SmlI: # [CTYRAG] # 118 236 894 # # TaiI: # [ACGT] # 3 112 1133 # # TaqI: # [TCGA] # 29 84 104 361 670 # # TaqII: # [CACCCA] # 373 875 # # TatI: # [WGTACW] # 89 1159 # # TfiI: # [GAWTC] # 21 119 148 199 761 # # TseI: # [GCWGC] # 83 1165 # # TsoI: # [TARCCA] # 585 663 # # Tsp45I: # [GTSAC] # 100 1148 # # Tsp4CI*: # [ACNGT] # 17 44 123 163 199 199 # 224 279 # # TspDTI: # [ATGAA] # 28 41 44 93 190 301 # 551 # # TspEI: # [AATT] # 9 10 20 22 25 26 # 32 35 36 60 70 116 # 124 282 381 # # TspGWI: # [ACGGA] # 79 401 768 # # TspRI: # [CASTGNN] # 105 118 486 539 # # XbaI: # [TCTAGA] # 429 819 # # XhoII: # [RGATCY] # 426 822 # # XmnI: # [GAANNNNTTC] # 41 1207 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1248 # Reported_sequences: 1 # Reported_hitcount: 253 #---------------------------------------