Restriction Fragments for ATG2/YNL242W



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ATG2/YNL242W on chromosome XIV from coordinates 191324 to 196102.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.12246.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 17 + BstEII GGTNACC 11 16 . . 12 16 + MaeIII GTNAC 11 16 . . 15 18 + SetI ASST 18 14 . . 16 19 + MnlI CCTC 26 25 . . 33 37 + TauI GCSGC 36 33 . . 33 37 + BlsI GCNGC 35 34 . . 33 37 + BisI GCNGC 34 35 . . 35 38 + CviJI RGCY 36 36 . . 36 40 + BlsI GCNGC 38 37 . . 36 40 + BisI GCNGC 37 38 . . 36 40 + TseI GCWGC 36 39 . . 33 36 - AciI CCGC 33 35 . . 36 40 - BbvI GCAGC 23 27 . . 47 50 + TaiI ACGT 50 46 . . 47 50 + SetI ASST 50 46 . . 47 50 + MaeII ACGT 47 49 . . 51 55 + Hin4II* CCTTC 61 60 . . 52 57 - Eco57MI CTGRAG 37 35 . . 57 67 + MwoI GCNNNNNNNGC 63 60 . . 52 57 - Eco57I CTGAAG 37 35 . . 59 64 + ApoI RAATTY 59 63 . . 60 63 + TspEI AATT 59 63 . . 98 101 + TaiI ACGT 101 97 . . 98 101 + SetI ASST 101 97 . . 98 101 + MaeII ACGT 98 100 . . 104 109 + SfeI* CTRYAG 104 108 . . 113 117 + DdeI CTNAG 113 116 . . 117 121 + Tsp45I GTSAC 116 121 . . 117 121 + MaeIII GTNAC 116 121 . . 133 136 + FatI CATG 132 136 . . 133 136 + NlaIII CATG 136 132 . . 133 136 + CviAII CATG 133 135 . . 136 145 + BsaBI GATNNNNATC 140 140 . . 141 145 + TfiI GAWTC 141 144 . . 141 145 + HinfI GANTC 141 144 . . 147 151 + Tsp45I GTSAC 146 151 . . 147 151 + MaeIII GTNAC 146 151 . . 157 160 + DpnI GATC 158 158 . . 157 160 + BstKTI GATC 159 157 . . 157 160 + MboI GATC 156 160 . . 173 176 + TaiI ACGT 176 172 . . 173 176 + SetI ASST 176 172 . . 173 176 + MaeII ACGT 173 175 . . 176 179 + CviRI* TGCA 177 177 . . 187 191 - Hin4II* CCTTC 181 180 . . 196 201 + Hpy166II GTNNAC 198 198 . . 196 201 + HindII GTYRAC 198 198 . . 203 206 + SetI ASST 206 202 . . 203 206 + CviJI RGCY 204 204 . . 203 206 + AluI AGCT 204 204 . . 211 214 + MseI TTAA 211 213 . . 215 218 + TspEI AATT 214 218 . . 217 220 + MseI TTAA 217 219 . . 220 224 + Tsp4CI* ACNGT 222 221 . . 221 229 + AlwNI CAGNNNCTG 226 223 . . 230 233 - AciI CCGC 230 232 . . 241 246 + ClaI ATCGAT 242 244 . . 242 245 + TaqI TCGA 242 244 . . 244 248 - BccI CCATC 238 239 . . 256 259 + MseI TTAA 256 258 . . 266 271 + Hpy178III* TCNNGA 267 269 . . 266 271 + BspHI TCATGA 266 270 . . 267 270 + FatI CATG 266 270 . . 267 270 + NlaIII CATG 270 266 . . 267 270 + CviAII CATG 267 269 . . 273 276 + SetI ASST 276 272 . . 285 291 + TspRI CASTGNN 291 282 . . 284 288 - BsrI ACTGG 284 282 . . 289 292 + CviJI RGCY 290 290 . . 306 310 + DdeI CTNAG 306 309 . . 306 310 + BspCNI CTCAG 319 317 . . 306 310 + BseMII CTCAG 320 318 . . 307 311 + Hpy188I TCNGA 309 308 . . 320 323 + MseI TTAA 320 322 . . 317 322 - TsoI TARCCA 307 305 . . 326 330 + SfaNI GCATC 335 339 . . 327 331 - FokI GGATG 313 317 . . 327 331 - BseGI GGATG 326 324 . . 333 338 + XhoII RGATCY 333 337 . . 333 338 + BglII AGATCT 333 337 . . 334 337 + DpnI GATC 335 335 . . 334 337 + BstKTI GATC 336 334 . . 334 337 + MboI GATC 333 337 . . 342 347 + EcoRI GAATTC 342 346 . . 342 347 + ApoI RAATTY 342 346 . . 343 346 + TspEI AATT 342 346 . . 346 349 + TaqI TCGA 346 348 . . 351 355 + MaeIII GTNAC 350 355 . . 356 359 + TspEI AATT 355 359 . . 364 367 + DpnI GATC 365 365 . . 364 367 + BstKTI GATC 366 364 . . 364 367 + MboI GATC 363 367 . . 364 368 - BinI* GGATC 358 359 . . 385 388 + SetI ASST 388 384 . . 387 390 + Csp6I GTAC 387 389 . . 387 390 + RsaI GTAC 388 388 . . 394 398 + MboII GAAGA 406 405 . . 400 410 + Hin4I GAYNNNNNVTC 391 386 423 418 406 409 + SetI ASST 409 405 . . 406 409 + CviJI RGCY 407 407 . . 406 409 + AluI AGCT 407 407 . . 407 413 + SapI GCTCTTC 414 417 . . 408 413 + Ksp632I* CTCTTC 414 417 . . 412 416 + Hpy188I TCNGA 414 413 . . 409 413 - MboII GAAGA 401 400 . . 410 415 - Eco57MI CTGRAG 395 393 . . 410 415 - Eco57I CTGAAG 395 393 . . 416 420 + Tsp4CI* ACNGT 418 417 . . 427 437 + Hin4I GAYNNNNNVTC 418 413 450 445 427 430 + DpnI GATC 428 428 . . 427 430 + BstKTI GATC 429 427 . . 427 430 + MboI GATC 426 430 . . 446 451 + HindIII AAGCTT 446 450 . . 447 450 + SetI ASST 450 446 . . 447 450 + CviJI RGCY 448 448 . . 447 450 + AluI AGCT 448 448 . . 459 462 + CviJI RGCY 460 460 . . 460 464 + TauI GCSGC 463 460 . . 460 464 + BlsI GCNGC 462 461 . . 460 464 + BisI GCNGC 461 462 . . 461 464 + AciI CCGC 461 463 . . 479 484 + Ksp632I* CTCTTC 485 488 . . 480 484 - MboII GAAGA 472 471 . . 492 495 - AciI CCGC 492 494 . . 500 506 + DraII RGGNCCY 501 504 . . 501 504 + CviJI RGCY 502 502 . . 501 504 + HaeIII GGCC 502 502 . . 501 505 + BmgT120I GGNCC 502 503 . . 501 505 + AsuI* GGNCC 501 504 . . 506 509 + MseI TTAA 506 508 . . 513 516 + CviJI RGCY 514 514 . . 515 521 + Bpu10I CCTNAGC 516 519 . . 516 520 + DdeI CTNAG 516 519 . . 524 527 + TspEI AATT 523 527 . . 538 541 + MseI TTAA 538 540 . . 545 548 + TaiI ACGT 548 544 . . 545 548 + SetI ASST 548 544 . . 545 548 + MaeII ACGT 545 547 . . 547 551 + MaeIII GTNAC 546 551 . . 555 558 + TaiI ACGT 558 554 . . 555 558 + SetI ASST 558 554 . . 555 558 + MaeII ACGT 555 557 . . 559 563 - TspDTI ATGAA 549 547 . . 571 580 + BsaBI GATNNNNATC 575 575 . . 576 581 + XhoII RGATCY 576 580 . . 577 580 + DpnI GATC 578 578 . . 577 580 + BstKTI GATC 579 577 . . 577 580 + MboI GATC 576 580 . . 580 584 + Hin4II* CCTTC 590 589 . . 577 581 - BinI* GGATC 571 572 . . 583 587 + Hpy188I TCNGA 585 584 . . 599 602 + SetI ASST 602 598 . . 598 601 - MnlI CCTC 591 590 . . 606 611 + XbaI TCTAGA 606 610 . . 602 606 - TspDTI ATGAA 592 590 . . 606 611 + Hpy178III* TCNNGA 607 609 . . 607 610 + MaeI CTAG 607 609 . . 629 634 + SfeI* CTRYAG 629 633 . . 634 638 + HgaI GACGC 643 648 . . 639 644 + ApoI RAATTY 639 643 . . 640 643 + TspEI AATT 639 643 . . 668 672 - HphI GGTGA 660 659 . . 677 680 + MnlI CCTC 687 686 . . 693 696 + SetI ASST 696 692 . . 693 696 + CviJI RGCY 694 694 . . 693 696 + AluI AGCT 694 694 . . 697 700 + Csp6I GTAC 697 699 . . 697 700 + RsaI GTAC 698 698 . . 703 707 + HinfI GANTC 703 706 . . 703 707 - PleI GAGTC 697 698 . . 703 707 - MlyI GAGTC 697 697 . . 713 716 + CviRI* TGCA 714 714 . . 714 718 + SfaNI GCATC 723 727 . . 715 719 - FokI GGATG 701 705 . . 715 719 - BseGI GGATG 714 712 . . 730 740 + Hin4I GAYNNNNNVTC 721 716 753 748 730 740 + Eam1105I GACNNNNNGTC 735 734 . . 733 736 + DpnI GATC 734 734 . . 733 736 + BstKTI GATC 735 733 . . 733 736 + MboI GATC 732 736 . . 738 742 + Tsp45I GTSAC 737 742 . . 738 742 + MaeIII GTNAC 737 742 . . 730 740 - Hin4I GAYNNNNNVTC 753 748 721 716 768 771 + CviJI RGCY 769 769 . . 766 769 - MnlI CCTC 759 758 . . 787 792 + HaeII RGCGCY 791 787 . . 788 791 + Hin6I GCGC 788 790 . . 788 791 + GlaI GCGC 789 789 . . 788 791 + HhaI GCGC 790 788 . . 791 796 + DsaI* CCRYGG 791 795 . . 791 796 + StyI CCWWGG 791 795 . . 791 796 + NcoI CCATGG 791 795 . . 791 796 + SecI* CCNNGG 791 795 . . 792 795 + FatI CATG 791 795 . . 792 795 + NlaIII CATG 795 791 . . 792 795 + CviAII CATG 792 794 . . 796 800 + MboII GAAGA 808 807 . . 796 801 - Ksp632I* CTCTTC 791 794 . . 796 802 - SapI GCTCTTC 791 794 . . 809 813 + TspDTI ATGAA 824 822 . . 811 815 + MboII GAAGA 823 822 . . 811 816 + BbvII* GAAGAC 818 822 . . 819 825 + TspRI CASTGNN 825 816 . . 821 826 + Hpy166II GTNNAC 823 823 . . 818 822 - BsrI ACTGG 818 816 . . 817 822 - BfiI ACTGGG 812 811 . . 832 836 + MaeIII GTNAC 831 836 . . 841 845 + MboII GAAGA 853 852 . . 862 865 + CviRI* TGCA 863 863 . . 868 872 + TfiI GAWTC 868 871 . . 868 872 + HinfI GANTC 868 871 . . 874 877 + MseI TTAA 874 876 . . 889 892 + TspEI AATT 888 892 . . 900 903 + SetI ASST 903 899 . . 900 903 + CviJI RGCY 901 901 . . 900 903 + AluI AGCT 901 901 . . 905 908 + MseI TTAA 905 907 . . 919 924 - TaqII GACCGA 909 907 . . 928 931 + DpnI GATC 929 929 . . 928 931 + BstKTI GATC 930 928 . . 928 931 + MboI GATC 927 931 . . 931 935 + DdeI CTNAG 931 934 . . 931 935 + BspCNI CTCAG 944 942 . . 931 935 + BseMII CTCAG 945 943 . . 932 936 + Hpy188I TCNGA 934 933 . . 943 948 + SspI AATATT 945 945 . . 960 965 + Hpy178III* TCNNGA 961 963 . . 963 967 + FokI GGATG 976 980 . . 963 967 + BseGI GGATG 969 967 . . 968 972 - TspDTI ATGAA 958 956 . . 987 990 + TspEI AATT 986 990 . . 992 995 + TaqI TCGA 992 994 . . 1014 1018 + MboII GAAGA 1026 1025 . . 1018 1023 + SspI AATATT 1020 1020 . . 1027 1032 + EcoRV GATATC 1029 1029 . . 1036 1040 + MaeIII GTNAC 1035 1040 . . 1042 1051 + MslI CAYNNNNRTG 1046 1046 . . 1047 1051 + FokI GGATG 1060 1064 . . 1047 1051 + BseGI GGATG 1053 1051 . . 1048 1052 - SfaNI GCATC 1038 1042 . . 1055 1059 + TspDTI ATGAA 1070 1068 . . 1059 1064 + HindIII AAGCTT 1059 1063 . . 1059 1069 + FalI AAGNNNNNCTT 1050 1045 1082 1077 1060 1063 + SetI ASST 1063 1059 . . 1060 1063 + CviJI RGCY 1061 1061 . . 1060 1063 + AluI AGCT 1061 1061 . . 1061 1071 + MwoI GCNNNNNNNGC 1067 1064 . . 1059 1069 - FalI AAGNNNNNCTT 1082 1077 1050 1045 1071 1075 + BssKI CCNGG 1070 1075 . . 1071 1075 + BseBI CCWGG 1072 1073 . . 1071 1075 + ScrFI CCNGG 1072 1073 . . 1071 1075 + EcoRII CCWGG 1070 1075 . . 1075 1079 + TfiI GAWTC 1075 1078 . . 1075 1079 + HinfI GANTC 1075 1078 . . 1087 1091 + Hin4II* CCTTC 1097 1096 . . 1092 1095 + SetI ASST 1095 1091 . . 1090 1094 - HphI GGTGA 1082 1081 . . 1104 1109 + NlaIV GGNNCC 1106 1106 . . 1108 1112 + BccI CCATC 1116 1117 . . 1113 1116 + SetI ASST 1116 1112 . . 1113 1116 + CviJI RGCY 1114 1114 . . 1113 1116 + AluI AGCT 1114 1114 . . 1120 1124 - MboII GAAGA 1112 1111 . . 1129 1134 + EcoRV GATATC 1131 1131 . . 1147 1150 + MseI TTAA 1147 1149 . . 1152 1156 + DdeI CTNAG 1152 1155 . . 1156 1159 + CviJI RGCY 1157 1157 . . 1156 1159 + HaeIII GGCC 1157 1157 . . 1162 1167 + EcoRV GATATC 1164 1164 . . 1168 1172 + Tsp4CI* ACNGT 1170 1169 . . 1173 1176 + TspEI AATT 1172 1176 . . 1179 1183 + MboII GAAGA 1191 1190 . . 1183 1186 + SetI ASST 1186 1182 . . 1183 1186 + CviJI RGCY 1184 1184 . . 1183 1186 + AluI AGCT 1184 1184 . . 1179 1184 - Ksp632I* CTCTTC 1174 1177 . . 1179 1185 - SapI GCTCTTC 1174 1177 . . 1186 1191 + AhaIII* TTTAAA 1188 1188 . . 1187 1190 + MseI TTAA 1187 1189 . . 1190 1193 + TspEI AATT 1189 1193 . . 1210 1213 + TspEI AATT 1209 1213 . . 1212 1216 - TspDTI ATGAA 1202 1200 . . 1231 1234 + CviJI RGCY 1232 1232 . . 1246 1249 + TspEI AATT 1245 1249 . . 1249 1252 + TaqI TCGA 1249 1251 . . 1251 1255 + PleI GAGTC 1259 1260 . . 1251 1255 + MlyI GAGTC 1260 1260 . . 1251 1255 + HinfI GANTC 1251 1254 . . 1263 1266 + TspEI AATT 1262 1266 . . 1266 1271 + TsoI TARCCA 1282 1280 . . 1267 1270 + CviJI RGCY 1268 1268 . . 1277 1281 + DdeI CTNAG 1277 1280 . . 1280 1283 + CviJI RGCY 1281 1281 . . 1283 1288 - BsrDI GCAATG 1282 1280 . . 1290 1296 + TspRI CASTGNN 1296 1287 . . 1291 1295 + BsrI ACTGG 1296 1294 . . 1292 1297 + Eco57MI CTGRAG 1313 1311 . . 1292 1297 + GsuI CTGGAG 1313 1311 . . 1306 1309 + MnlI CCTC 1316 1315 . . 1313 1317 + SfaNI GCATC 1322 1326 . . 1314 1319 - BtgZI GCGATG 1299 1303 . . 1321 1324 + MseI TTAA 1321 1323 . . 1332 1336 + Hpy99I CGWCG 1336 1331 . . 1339 1342 + MaeI CTAG 1339 1341 . . 1356 1361 + Hpy178III* TCNNGA 1357 1359 . . 1356 1361 + BspHI TCATGA 1356 1360 . . 1357 1360 + FatI CATG 1356 1360 . . 1357 1360 + NlaIII CATG 1360 1356 . . 1357 1360 + CviAII CATG 1357 1359 . . 1360 1364 - BccI CCATC 1354 1355 . . 1368 1373 + MstI* TGCGCA 1370 1370 . . 1369 1372 + Hin6I GCGC 1369 1371 . . 1369 1372 + GlaI GCGC 1370 1370 . . 1369 1372 + HhaI GCGC 1371 1369 . . 1365 1370 - BsrDI GCAATG 1364 1362 . . 1378 1383 + AhaIII* TTTAAA 1380 1380 . . 1379 1382 + MseI TTAA 1379 1381 . . 1419 1423 + Hpy188I TCNGA 1421 1420 . . 1436 1440 + FokI GGATG 1449 1453 . . 1436 1440 + BseGI GGATG 1442 1440 . . 1437 1447 + Hin4I GAYNNNNNVTC 1428 1423 1460 1455 1444 1447 + MnlI CCTC 1454 1453 . . 1440 1444 - SfaNI GCATC 1430 1434 . . 1453 1457 + SfaNI GCATC 1462 1466 . . 1456 1460 + Hpy188I TCNGA 1458 1457 . . 1458 1463 - BciVI GTATCC 1452 1451 . . 1469 1473 + BmgT120I GGNCC 1470 1471 . . 1469 1473 + AvaII GGWCC 1469 1472 . . 1469 1473 + AsuI* GGNCC 1469 1472 . . 1471 1475 + Tsp4CI* ACNGT 1473 1472 . . 1474 1477 + Csp6I GTAC 1474 1476 . . 1474 1477 + RsaI GTAC 1475 1475 . . 1483 1486 + SetI ASST 1486 1482 . . 1484 1487 + MnlI CCTC 1494 1493 . . 1492 1497 + BtsI GCAGTG 1499 1497 . . 1493 1499 + TspRI CASTGNN 1499 1490 . . 1507 1512 + SmlI CTYRAG 1507 1511 . . 1507 1512 + AflII CTTAAG 1507 1511 . . 1508 1511 + MseI TTAA 1508 1510 . . 1536 1540 + BssKI CCNGG 1535 1540 . . 1536 1540 + BseBI CCWGG 1537 1538 . . 1536 1540 + ScrFI CCNGG 1537 1538 . . 1536 1540 + EcoRII CCWGG 1535 1540 . . 1538 1541 + SetI ASST 1541 1537 . . 1547 1551 + Hin4II* CCTTC 1557 1556 . . 1543 1547 - HphI GGTGA 1535 1534 . . 1549 1553 - TspDTI ATGAA 1539 1537 . . 1558 1562 + HinfI GANTC 1558 1561 . . 1558 1562 - PleI GAGTC 1552 1553 . . 1558 1562 - MlyI GAGTC 1552 1552 . . 1565 1569 + Tsp4CI* ACNGT 1567 1566 . . 1574 1579 + TatI WGTACW 1574 1578 . . 1574 1579 + Bsp1407I TGTACA 1574 1578 . . 1575 1578 + Csp6I GTAC 1575 1577 . . 1575 1578 + RsaI GTAC 1576 1576 . . 1583 1586 + SetI ASST 1586 1582 . . 1583 1586 + CviJI RGCY 1584 1584 . . 1583 1586 + AluI AGCT 1584 1584 . . 1588 1593 + Hpy166II GTNNAC 1590 1590 . . 1588 1593 + AccI GTMKAC 1589 1591 . . 1592 1595 + TaiI ACGT 1595 1591 . . 1592 1595 + SetI ASST 1595 1591 . . 1592 1595 + MaeII ACGT 1592 1594 . . 1597 1602 + AhaIII* TTTAAA 1599 1599 . . 1598 1601 + MseI TTAA 1598 1600 . . 1608 1611 + SetI ASST 1611 1607 . . 1609 1613 + Hin4II* CCTTC 1619 1618 . . 1617 1622 + Hpy166II GTNNAC 1619 1619 . . 1617 1622 + SduI GDGCHC 1621 1617 . . 1617 1622 + BseSI GKGCMC 1621 1617 . . 1617 1622 + ApaLI GTGCAC 1617 1621 . . 1617 1622 + HgiAI* GWGCWC 1621 1617 . . 1618 1621 + CviRI* TGCA 1619 1619 . . 1626 1631 + SspI AATATT 1628 1628 . . 1632 1640 + Tth111I GACNNNGTC 1635 1636 . . 1639 1642 + TaqI TCGA 1639 1641 . . 1650 1653 + DpnI GATC 1651 1651 . . 1650 1653 + BstKTI GATC 1652 1650 . . 1650 1653 + MboI GATC 1649 1653 . . 1652 1657 + Hpy178III* TCNNGA 1653 1655 . . 1654 1657 + TaqI TCGA 1654 1656 . . 1656 1668 + AloI GAACNNNNNNTCC 1648 1643 1680 1675 1661 1664 + CviRI* TGCA 1662 1662 . . 1669 1672 + MnlI CCTC 1679 1678 . . 1677 1681 + BsrI ACTGG 1682 1680 . . 1677 1682 + BfiI ACTGGG 1687 1686 . . 1685 1690 + NdeI CATATG 1686 1688 . . 1683 1687 - TspDTI ATGAA 1673 1671 . . 1707 1712 + HaeII RGCGCY 1711 1707 . . 1707 1712 + Eco47III AGCGCT 1709 1709 . . 1708 1711 + Hin6I GCGC 1708 1710 . . 1708 1711 + GlaI GCGC 1709 1709 . . 1708 1711 + HhaI GCGC 1710 1708 . . 1713 1716 + TspEI AATT 1712 1716 . . 1729 1732 + FatI CATG 1728 1732 . . 1729 1732 + NlaIII CATG 1732 1728 . . 1729 1732 + CviAII CATG 1729 1731 . . 1731 1734 + CviRI* TGCA 1732 1732 . . 1754 1757 + MseI TTAA 1754 1756 . . 1759 1763 + MboII GAAGA 1771 1770 . . 1763 1768 + SspI AATATT 1765 1765 . . 1768 1773 + Hpy178III* TCNNGA 1769 1771 . . 1776 1781 + TatI WGTACW 1776 1780 . . 1777 1780 + Csp6I GTAC 1777 1779 . . 1777 1780 + RsaI GTAC 1778 1778 . . 1781 1784 + TspEI AATT 1780 1784 . . 1787 1790 + TaqI TCGA 1787 1789 . . 1789 1793 + MboII GAAGA 1801 1800 . . 1819 1823 + TspGWI ACGGA 1834 1832 . . 1822 1826 + TfiI GAWTC 1822 1825 . . 1822 1826 + HinfI GANTC 1822 1825 . . 1832 1836 + Tsp4CI* ACNGT 1834 1833 . . 1833 1839 + TspRI CASTGNN 1839 1830 . . 1840 1845 + TatI WGTACW 1840 1844 . . 1840 1845 + Bsp1407I TGTACA 1840 1844 . . 1841 1844 + Csp6I GTAC 1841 1843 . . 1841 1844 + RsaI GTAC 1842 1842 . . 1859 1869 + MwoI GCNNNNNNNGC 1865 1862 . . 1867 1870 + CviJI RGCY 1868 1868 . . 1867 1872 + NlaIV GGNNCC 1869 1869 . . 1865 1868 - MnlI CCTC 1858 1857 . . 1877 1880 + CviRI* TGCA 1878 1878 . . 1889 1893 + Hpy188I TCNGA 1891 1890 . . 1893 1898 + TatI WGTACW 1893 1897 . . 1893 1898 + ScaI AGTACT 1895 1895 . . 1894 1897 + Csp6I GTAC 1894 1896 . . 1894 1897 + RsaI GTAC 1895 1895 . . 1898 1901 + MseI TTAA 1898 1900 . . 1900 1903 + TspEI AATT 1899 1903 . . 1903 1907 - MboII GAAGA 1895 1894 . . 1907 1911 + BssKI CCNGG 1906 1911 . . 1907 1911 + BseBI CCWGG 1908 1909 . . 1907 1911 + ScrFI CCNGG 1908 1909 . . 1907 1911 + EcoRII CCWGG 1906 1911 . . 1909 1912 + SetI ASST 1912 1908 . . 1915 1920 + Hpy166II GTNNAC 1917 1917 . . 1934 1937 + AciI CCGC 1934 1936 . . 1939 1942 + TspEI AATT 1938 1942 . . 1948 1953 + SmlI CTYRAG 1948 1952 . . 1949 1954 + Hpy178III* TCNNGA 1950 1952 . . 1948 1953 - Bce83I* CTTGAG 1933 1931 . . 1957 1961 + TfiI GAWTC 1957 1960 . . 1957 1961 + HinfI GANTC 1957 1960 . . 1962 1965 + FatI CATG 1961 1965 . . 1962 1965 + NlaIII CATG 1965 1961 . . 1962 1965 + CviAII CATG 1962 1964 . . 1963 1967 + TspDTI ATGAA 1978 1976 . . 1969 1972 + MseI TTAA 1969 1971 . . 1978 1982 + Hpy188I TCNGA 1980 1979 . . 1983 1986 + TspEI AATT 1982 1986 . . 1990 1994 + BmgT120I GGNCC 1991 1992 . . 1990 1994 + AsuI* GGNCC 1990 1993 . . 1991 1994 + CviJI RGCY 1992 1992 . . 1991 1994 + HaeIII GGCC 1992 1992 . . 1992 1996 + TauI GCSGC 1995 1992 . . 1992 1996 + BlsI GCNGC 1994 1993 . . 1992 1996 + BisI GCNGC 1993 1994 . . 1993 2003 + BsiYI* CCNNNNNNNGG 1999 1996 . . 1993 1996 + AciI CCGC 1993 1995 . . 1997 2000 + TspEI AATT 1996 2000 . . 2029 2032 + TspEI AATT 2028 2032 . . 2044 2048 + TspGWI ACGGA 2059 2057 . . 2046 2050 + FokI GGATG 2059 2063 . . 2046 2050 + BseGI GGATG 2052 2050 . . 2050 2054 + HinfI GANTC 2050 2053 . . 2050 2054 - PleI GAGTC 2044 2045 . . 2050 2054 - MlyI GAGTC 2044 2044 . . 2058 2061 + TspEI AATT 2057 2061 . . 2080 2083 + MseI TTAA 2080 2082 . . 2084 2087 + SetI ASST 2087 2083 . . 2097 2105 + Tth111I GACNNNGTC 2100 2101 . . 2100 2104 + Tsp4CI* ACNGT 2102 2101 . . 2114 2117 + CviJI RGCY 2115 2115 . . 2127 2130 + FatI CATG 2126 2130 . . 2127 2130 + NlaIII CATG 2130 2126 . . 2127 2130 + CviAII CATG 2127 2129 . . 2152 2157 + Hpy166II GTNNAC 2154 2154 . . 2152 2157 + HpaI GTTAAC 2154 2154 . . 2152 2157 + HindII GTYRAC 2154 2154 . . 2153 2156 + MseI TTAA 2153 2155 . . 2160 2163 + SetI ASST 2163 2159 . . 2163 2168 + TatI WGTACW 2163 2167 . . 2164 2167 + Csp6I GTAC 2164 2166 . . 2164 2167 + RsaI GTAC 2165 2165 . . 2164 2176 - PsrI GAACNNNNNNTAC 2188 2183 2156 2151 2174 2178 - TspDTI ATGAA 2164 2162 . . 2180 2184 - Hin4II* CCTTC 2174 2173 . . 2197 2200 + MseI TTAA 2197 2199 . . 2210 2213 + MseI TTAA 2210 2212 . . 2227 2236 + MslI CAYNNNNRTG 2231 2231 . . 2235 2238 + CviRI* TGCA 2236 2236 . . 2239 2242 + SetI ASST 2242 2238 . . 2242 2247 - TsoI TARCCA 2232 2230 . . 2269 2272 + CviJI RGCY 2270 2270 . . 2269 2272 + HaeIII GGCC 2270 2270 . . 2269 2273 + BmgT120I GGNCC 2270 2271 . . 2269 2273 + AsuI* GGNCC 2269 2272 . . 2271 2275 + BssKI CCNGG 2270 2275 . . 2271 2275 + ScrFI CCNGG 2272 2273 . . 2271 2275 + CauII* CCSGG 2272 2273 . . 2272 2275 + HpaII CCGG 2272 2274 . . 2282 2286 - BsrI ACTGG 2282 2280 . . 2294 2298 + TspDTI ATGAA 2309 2307 . . 2298 2301 + SetI ASST 2301 2297 . . 2311 2316 + HindIII AAGCTT 2311 2315 . . 2312 2315 + SetI ASST 2315 2311 . . 2312 2315 + CviJI RGCY 2313 2313 . . 2312 2315 + AluI AGCT 2313 2313 . . 2314 2318 + DdeI CTNAG 2314 2317 . . 2329 2339 + BsiYI* CCNNNNNNNGG 2335 2332 . . 2335 2340 + Hpy178III* TCNNGA 2336 2338 . . 2333 2338 - Eco57MI CTGRAG 2318 2316 . . 2333 2338 - Eco57I CTGAAG 2318 2316 . . 2344 2350 + PasI CCCWGGG 2345 2348 . . 2344 2349 + SecI* CCNNGG 2344 2348 . . 2345 2349 + BssKI CCNGG 2344 2349 . . 2345 2349 + BseBI CCWGG 2346 2347 . . 2345 2350 + SecI* CCNNGG 2345 2349 . . 2345 2349 + ScrFI CCNGG 2346 2347 . . 2345 2349 + EcoRII CCWGG 2344 2349 . . 2361 2366 + Hpy178III* TCNNGA 2362 2364 . . 2376 2379 + TspEI AATT 2375 2379 . . 2381 2384 + CviRI* TGCA 2382 2382 . . 2380 2385 - BsgI GTGCAG 2365 2363 . . 2390 2394 + Hpy188I TCNGA 2392 2391 . . 2395 2398 + MseI TTAA 2395 2397 . . 2415 2420 + TatI WGTACW 2415 2419 . . 2416 2419 + Csp6I GTAC 2416 2418 . . 2416 2419 + RsaI GTAC 2417 2417 . . 2434 2438 + BsrI ACTGG 2439 2437 . . 2445 2449 - TspDTI ATGAA 2435 2433 . . 2455 2458 + AciI CCGC 2455 2457 . . 2457 2467 + MwoI GCNNNNNNNGC 2463 2460 . . 2457 2462 + Cac8I GCNNGC 2459 2459 . . 2464 2469 + HindIII AAGCTT 2464 2468 . . 2465 2468 + SetI ASST 2468 2464 . . 2465 2468 + CviJI RGCY 2466 2466 . . 2465 2468 + AluI AGCT 2466 2466 . . 2488 2493 + Ksp632I* CTCTTC 2494 2497 . . 2489 2493 - MboII GAAGA 2481 2480 . . 2500 2509 + BsaBI GATNNNNATC 2504 2504 . . 2508 2512 + Hpy188I TCNGA 2510 2509 . . 2511 2516 + ApoI RAATTY 2511 2515 . . 2512 2515 + TspEI AATT 2511 2515 . . 2519 2524 + ApoI RAATTY 2519 2523 . . 2520 2523 + TspEI AATT 2519 2523 . . 2537 2541 + BsrI ACTGG 2542 2540 . . 2540 2543 + CviJI RGCY 2541 2541 . . 2540 2543 + HaeIII GGCC 2541 2541 . . 2543 2546 + MaeI CTAG 2543 2545 . . 2553 2558 + ApoI RAATTY 2553 2557 . . 2554 2557 + TspEI AATT 2553 2557 . . 2571 2574 + SetI ASST 2574 2570 . . 2580 2583 + CviRI* TGCA 2581 2581 . . 2604 2609 + ApoI RAATTY 2604 2608 . . 2605 2608 + TspEI AATT 2604 2608 . . 2611 2614 + MseI TTAA 2611 2613 . . 2623 2627 + Hpy188I TCNGA 2625 2624 . . 2629 2634 + NlaIV GGNNCC 2631 2631 . . 2629 2634 + HgiCI* GGYRCC 2629 2633 . . 2630 2635 + SduI GDGCHC 2634 2630 . . 2626 2630 - BccI CCATC 2620 2621 . . 2630 2635 + BseSI GKGCMC 2634 2630 . . 2634 2644 + BsiYI* CCNNNNNNNGG 2640 2637 . . 2660 2670 + FalI AAGNNNNNCTT 2651 2646 2683 2678 2660 2670 - FalI AAGNNNNNCTT 2683 2678 2651 2646 2670 2674 - TspDTI ATGAA 2660 2658 . . 2678 2681 + Hin6I GCGC 2678 2680 . . 2678 2681 + GlaI GCGC 2679 2679 . . 2678 2681 + HhaI GCGC 2680 2678 . . 2679 2682 + FnuDII* CGCG 2680 2680 . . 2683 2687 + TfiI GAWTC 2683 2686 . . 2683 2687 + HinfI GANTC 2683 2686 . . 2680 2683 - AciI CCGC 2680 2682 . . 2705 2708 + SetI ASST 2708 2704 . . 2705 2708 + CviJI RGCY 2706 2706 . . 2705 2708 + AluI AGCT 2706 2706 . . 2718 2723 + AhaIII* TTTAAA 2720 2720 . . 2719 2722 + MseI TTAA 2719 2721 . . 2728 2731 + MnlI CCTC 2738 2737 . . 2729 2733 + DdeI CTNAG 2729 2732 . . 2729 2733 + BspCNI CTCAG 2742 2740 . . 2729 2733 + BseMII CTCAG 2743 2741 . . 2730 2734 + Hpy188I TCNGA 2732 2731 . . 2734 2737 + TaiI ACGT 2737 2733 . . 2734 2737 + SetI ASST 2737 2733 . . 2734 2737 + MaeII ACGT 2734 2736 . . 2739 2744 + Hpy178III* TCNNGA 2740 2742 . . 2746 2749 - MnlI CCTC 2739 2738 . . 2752 2757 + ApoI RAATTY 2752 2756 . . 2753 2756 + TspEI AATT 2752 2756 . . 2779 2784 + ClaI ATCGAT 2780 2782 . . 2780 2783 + TaqI TCGA 2780 2782 . . 2797 2802 + ClaI ATCGAT 2798 2800 . . 2798 2801 + TaqI TCGA 2798 2800 . . 2800 2809 + BsaBI GATNNNNATC 2804 2804 . . 2806 2809 + DpnI GATC 2807 2807 . . 2806 2809 + BstKTI GATC 2808 2806 . . 2806 2809 + MboI GATC 2805 2809 . . 2815 2818 + TspEI AATT 2814 2818 . . 2822 2826 + Hin4II* CCTTC 2832 2831 . . 2822 2832 + BsiYI* CCNNNNNNNGG 2828 2825 . . 2824 2828 - TspDTI ATGAA 2814 2812 . . 2828 2834 + PfoI TCCNGGA 2828 2833 . . 2829 2833 + BssKI CCNGG 2828 2833 . . 2829 2832 + HpaII CCGG 2829 2831 . . 2829 2833 + ScrFI CCNGG 2830 2831 . . 2829 2833 + CauII* CCSGG 2830 2831 . . 2826 2830 - FokI GGATG 2812 2816 . . 2826 2830 - BseGI GGATG 2825 2823 . . 2842 2851 + MslI CAYNNNNRTG 2846 2846 . . 2851 2861 + Hin4I GAYNNNNNVTC 2842 2837 2874 2869 2857 2861 + HinfI GANTC 2857 2860 . . 2859 2863 + DdeI CTNAG 2859 2862 . . 2859 2863 + BspCNI CTCAG 2872 2870 . . 2859 2863 + BseMII CTCAG 2873 2871 . . 2861 2867 + TspRI CASTGNN 2867 2858 . . 2857 2861 - PleI GAGTC 2851 2852 . . 2857 2861 - MlyI GAGTC 2851 2851 . . 2864 2867 + CviRI* TGCA 2865 2865 . . 2872 2877 + Hpy166II GTNNAC 2874 2874 . . 2872 2877 + AccI GTMKAC 2873 2875 . . 2876 2880 + Tsp4CI* ACNGT 2878 2877 . . 2883 2888 + Hpy178III* TCNNGA 2884 2886 . . 2885 2888 + TaqI TCGA 2885 2887 . . 2895 2900 + Hpy178III* TCNNGA 2896 2898 . . 2896 2901 + BsiI* CACGAG 2896 2900 . . 2911 2916 + NlaIV GGNNCC 2913 2913 . . 2911 2916 + HgiCI* GGYRCC 2911 2915 . . 2921 2926 + Hpy166II GTNNAC 2923 2923 . . 2930 2933 + MnlI CCTC 2940 2939 . . 2933 2938 + SmlI CTYRAG 2933 2937 . . 2937 2940 + SetI ASST 2940 2936 . . 2933 2938 - Bce83I* CTTGAG 2918 2916 . . 2942 2947 + NdeI CATATG 2943 2945 . . 2940 2944 - TspDTI ATGAA 2930 2928 . . 2945 2949 + TspDTI ATGAA 2960 2958 . . 2953 2963 + Hin4I GAYNNNNNVTC 2944 2939 2976 2971 2954 2958 + Tsp4CI* ACNGT 2956 2955 . . 2960 2963 + MnlI CCTC 2970 2969 . . 2962 2967 + Hpy178III* TCNNGA 2963 2965 . . 2976 2979 + SetI ASST 2979 2975 . . 2995 2998 + FatI CATG 2994 2998 . . 2995 2998 + NlaIII CATG 2998 2994 . . 2995 2998 + CviAII CATG 2995 2997 . . 3006 3011 + XbaI TCTAGA 3006 3010 . . 3006 3011 + Hpy178III* TCNNGA 3007 3009 . . 3007 3010 + MaeI CTAG 3007 3009 . . 3010 3014 + HinfI GANTC 3010 3013 . . 3010 3014 - PleI GAGTC 3004 3005 . . 3010 3014 - MlyI GAGTC 3004 3004 . . 3016 3022 + EspI* GCTNAGC 3017 3020 . . 3017 3021 + DdeI CTNAG 3017 3020 . . 3017 3021 + BspCNI CTCAG 3030 3028 . . 3017 3021 + BseMII CTCAG 3031 3029 . . 3020 3023 + CviJI RGCY 3021 3021 . . 3028 3032 + MboII GAAGA 3040 3039 . . 3031 3035 + MboII GAAGA 3043 3042 . . 3050 3053 + MnlI CCTC 3060 3059 . . 3069 3073 + FokI GGATG 3082 3086 . . 3069 3073 + BseGI GGATG 3075 3073 . . 3098 3101 + TaqI TCGA 3098 3100 . . 3110 3115 + PsiI TTATAA 3112 3112 . . 3122 3125 + TaqI TCGA 3122 3124 . . 3124 3128 - BccI CCATC 3118 3119 . . 3146 3149 + FnuDII* CGCG 3147 3147 . . 3147 3150 - AciI CCGC 3147 3149 . . 3151 3156 + ApoI RAATTY 3151 3155 . . 3152 3155 + TspEI AATT 3151 3155 . . 3166 3170 + Tsp4CI* ACNGT 3168 3167 . . 3177 3181 + BsrI ACTGG 3182 3180 . . 3187 3192 + SacI GAGCTC 3191 3187 . . 3187 3192 + Ecl136II GAGCTC 3189 3189 . . 3187 3192 + SduI GDGCHC 3191 3187 . . 3187 3192 + HgiJII* GRGCYC 3191 3187 . . 3187 3192 + HgiAI* GWGCWC 3191 3187 . . 3188 3191 + SetI ASST 3191 3187 . . 3188 3191 + CviJI RGCY 3189 3189 . . 3188 3191 + AluI AGCT 3189 3189 . . 3190 3194 + DdeI CTNAG 3190 3193 . . 3190 3194 + BspCNI CTCAG 3203 3201 . . 3190 3194 + BseMII CTCAG 3204 3202 . . 3199 3202 - AciI CCGC 3199 3201 . . 3205 3210 + Cac8I GCNNGC 3207 3207 . . 3213 3218 + AsuII TTCGAA 3214 3216 . . 3214 3217 + TaqI TCGA 3214 3216 . . 3205 3216 - BcgI CGANNNNNNTGC 3228 3226 3194 3192 3226 3229 + Csp6I GTAC 3226 3228 . . 3226 3229 + RsaI GTAC 3227 3227 . . 3231 3241 + BsiYI* CCNNNNNNNGG 3237 3234 . . 3235 3239 + Hpy188I TCNGA 3237 3236 . . 3238 3241 - MnlI CCTC 3231 3230 . . 3244 3249 + SspI AATATT 3246 3246 . . 3253 3257 + HinfI GANTC 3253 3256 . . 3253 3257 - PleI GAGTC 3247 3248 . . 3253 3257 - MlyI GAGTC 3247 3247 . . 3286 3290 + Tsp45I GTSAC 3285 3290 . . 3286 3290 + MaeIII GTNAC 3285 3290 . . 3299 3303 + MboII GAAGA 3311 3310 . . 3306 3311 + Hpy178III* TCNNGA 3307 3309 . . 3297 3307 - Hin4I GAYNNNNNVTC 3320 3315 3288 3283 3313 3317 + HphI GGTGA 3325 3324 . . 3310 3314 - BccI CCATC 3304 3305 . . 3317 3322 + XhoII RGATCY 3317 3321 . . 3318 3321 + DpnI GATC 3319 3319 . . 3318 3321 + BstKTI GATC 3320 3318 . . 3318 3321 + MboI GATC 3317 3321 . . 3321 3325 + BssKI CCNGG 3320 3325 . . 3321 3325 + BseBI CCWGG 3322 3323 . . 3321 3326 + SecI* CCNNGG 3321 3325 . . 3321 3325 + ScrFI CCNGG 3322 3323 . . 3321 3325 + EcoRII CCWGG 3320 3325 . . 3318 3322 - BinI* GGATC 3312 3313 . . 3330 3334 + Hpy188I TCNGA 3332 3331 . . 3333 3338 + EcoRI GAATTC 3333 3337 . . 3333 3342 + XmnI GAANNNNTTC 3337 3337 . . 3333 3338 + ApoI RAATTY 3333 3337 . . 3334 3337 + TspEI AATT 3333 3337 . . 3338 3342 + Hin4II* CCTTC 3348 3347 . . 3343 3347 + Hpy188I TCNGA 3345 3344 . . 3349 3353 + BsmAI GTCTC 3354 3358 . . 3359 3362 + MseI TTAA 3359 3361 . . 3361 3364 + TspEI AATT 3360 3364 . . 3369 3372 + SetI ASST 3372 3368 . . 3370 3374 + Hin4II* CCTTC 3380 3379 . . 3374 3378 + DdeI CTNAG 3374 3377 . . 3387 3390 + CviJI RGCY 3388 3388 . . 3393 3397 + TfiI GAWTC 3393 3396 . . 3393 3397 + HinfI GANTC 3393 3396 . . 3397 3402 + MfeI CAATTG 3397 3401 . . 3398 3401 + TspEI AATT 3397 3401 . . 3408 3413 + TatI WGTACW 3408 3412 . . 3409 3412 + Csp6I GTAC 3409 3411 . . 3409 3412 + RsaI GTAC 3410 3410 . . 3411 3416 + BspLU11I* ACATGT 3411 3415 . . 3411 3416 + NspI RCATGY 3415 3411 . . 3411 3416 + AflIII ACRYGT 3411 3415 . . 3412 3415 + FatI CATG 3411 3415 . . 3412 3415 + NlaIII CATG 3415 3411 . . 3412 3415 + CviAII CATG 3412 3414 . . 3418 3421 + TspEI AATT 3417 3421 . . 3423 3427 + MboII GAAGA 3435 3434 . . 3427 3430 + TspEI AATT 3426 3430 . . 3432 3435 + TaqI TCGA 3432 3434 . . 3440 3444 + TspDTI ATGAA 3455 3453 . . 3451 3455 + PleI GAGTC 3459 3460 . . 3451 3455 + MlyI GAGTC 3460 3460 . . 3451 3455 + HinfI GANTC 3451 3454 . . 3451 3461 + BplI GAGNNNNNCTC 3442 3437 3474 3469 3460 3464 + Hpy188I TCNGA 3462 3461 . . 3451 3461 - BplI GAGNNNNNCTC 3474 3469 3442 3437 3466 3469 + TspEI AATT 3465 3469 . . 3508 3513 + ApoI RAATTY 3508 3512 . . 3509 3512 + TspEI AATT 3508 3512 . . 3516 3519 + TspEI AATT 3515 3519 . . 3520 3525 + ClaI ATCGAT 3521 3523 . . 3521 3524 + TaqI TCGA 3521 3523 . . 3534 3539 + BtrI CACGTC 3536 3536 . . 3535 3538 + TaiI ACGT 3538 3534 . . 3535 3538 + SetI ASST 3538 3534 . . 3535 3538 + MaeII ACGT 3535 3537 . . 3537 3542 + Hpy166II GTNNAC 3539 3539 . . 3541 3544 + SetI ASST 3544 3540 . . 3542 3546 + BssKI CCNGG 3541 3546 . . 3542 3546 + BseBI CCWGG 3543 3544 . . 3542 3546 + ScrFI CCNGG 3543 3544 . . 3542 3546 + EcoRII CCWGG 3541 3546 . . 3550 3555 + ApoI RAATTY 3550 3554 . . 3551 3554 + TspEI AATT 3550 3554 . . 3556 3560 + MaeIII GTNAC 3555 3560 . . 3576 3580 + Tsp4CI* ACNGT 3578 3577 . . 3577 3582 + DsaI* CCRYGG 3577 3581 . . 3577 3582 + SecI* CCNNGG 3577 3581 . . 3580 3585 + BalI TGGCCA 3582 3582 . . 3580 3585 + CfrI YGGCCR 3580 3584 . . 3581 3584 + CviJI RGCY 3582 3582 . . 3581 3584 + HaeIII GGCC 3582 3582 . . 3587 3591 + Tsp4CI* ACNGT 3589 3588 . . 3594 3598 + Hpy188I TCNGA 3596 3595 . . 3606 3611 + Hpy178III* TCNNGA 3607 3609 . . 3603 3607 - MboII GAAGA 3595 3594 . . 3609 3613 + BmgT120I GGNCC 3610 3611 . . 3609 3613 + AvaII GGWCC 3609 3612 . . 3609 3613 + AsuI* GGNCC 3609 3612 . . 3611 3614 + SetI ASST 3614 3610 . . 3612 3616 + BssKI CCNGG 3611 3616 . . 3612 3616 + BseBI CCWGG 3613 3614 . . 3612 3616 + ScrFI CCNGG 3613 3614 . . 3612 3616 + EcoRII CCWGG 3611 3616 . . 3616 3621 + ApoI RAATTY 3616 3620 . . 3617 3620 + TspEI AATT 3616 3620 . . 3624 3627 + HpaII CCGG 3624 3626 . . 3625 3630 + McrI* CGRYCG 3628 3626 . . 3625 3630 + XmaIII* CGGCCG 3625 3629 . . 3625 3630 + CfrI YGGCCR 3625 3629 . . 3626 3629 + CviJI RGCY 3627 3627 . . 3626 3629 + HaeIII GGCC 3627 3627 . . 3629 3634 + McrI* CGRYCG 3632 3630 . . 3629 3634 + PvuI CGATCG 3632 3630 . . 3630 3633 + DpnI GATC 3631 3631 . . 3630 3633 + BstKTI GATC 3632 3630 . . 3630 3633 + MboI GATC 3629 3633 . . 3632 3635 + TaqI TCGA 3632 3634 . . 3641 3644 + CviRI* TGCA 3642 3642 . . 3642 3647 + Cac8I GCNNGC 3644 3644 . . 3646 3651 + BtsI GCAGTG 3653 3651 . . 3647 3653 + TspRI CASTGNN 3653 3644 . . 3653 3656 + TspEI AATT 3652 3656 . . 3660 3663 + FatI CATG 3659 3663 . . 3660 3663 + NlaIII CATG 3663 3659 . . 3660 3663 + CviAII CATG 3660 3662 . . 3662 3665 + CviRI* TGCA 3663 3663 . . 3665 3668 + TspEI AATT 3664 3668 . . 3667 3670 + MseI TTAA 3667 3669 . . 3676 3679 + Hin6I GCGC 3676 3678 . . 3676 3679 + GlaI GCGC 3677 3677 . . 3676 3679 + HhaI GCGC 3678 3676 . . 3687 3691 + Hin4II* CCTTC 3697 3696 . . 3687 3697 + BsiYI* CCNNNNNNNGG 3693 3690 . . 3691 3696 + BsaAI YACGTR 3693 3693 . . 3691 3696 + PmaCI CACGTG 3693 3693 . . 3692 3695 + TaiI ACGT 3695 3691 . . 3692 3695 + SetI ASST 3695 3691 . . 3692 3695 + MaeII ACGT 3692 3694 . . 3696 3700 + BinI* GGATC 3704 3705 . . 3697 3700 + DpnI GATC 3698 3698 . . 3697 3700 + BstKTI GATC 3699 3697 . . 3697 3700 + MboI GATC 3696 3700 . . 3699 3704 + Hpy178III* TCNNGA 3700 3702 . . 3724 3727 + SetI ASST 3727 3723 . . 3729 3733 + DdeI CTNAG 3729 3732 . . 3755 3758 + TspEI AATT 3754 3758 . . 3756 3761 + VspI ATTAAT 3757 3759 . . 3757 3760 + MseI TTAA 3757 3759 . . 3759 3762 + TspEI AATT 3758 3762 . . 3763 3772 + BsaBI GATNNNNATC 3767 3767 . . 3768 3773 + BciVI GTATCC 3779 3778 . . 3771 3776 + Hpy178III* TCNNGA 3772 3774 . . 3793 3798 + ApoI RAATTY 3793 3797 . . 3794 3797 + TspEI AATT 3793 3797 . . 3805 3808 + TspEI AATT 3804 3808 . . 3810 3813 + TspEI AATT 3809 3813 . . 3812 3815 + MseI TTAA 3812 3814 . . 3817 3820 + MseI TTAA 3817 3819 . . 3828 3833 + PsiI TTATAA 3830 3830 . . 3833 3836 + CviJI RGCY 3834 3834 . . 3853 3856 - AciI CCGC 3853 3855 . . 3853 3857 - FauI CCCGC 3846 3848 . . 3859 3862 + MseI TTAA 3859 3861 . . 3863 3866 + DpnI GATC 3864 3864 . . 3863 3866 + BstKTI GATC 3865 3863 . . 3863 3866 + MboI GATC 3862 3866 . . 3867 3871 + BmgT120I GGNCC 3868 3869 . . 3867 3871 + AsuI* GGNCC 3867 3870 . . 3868 3871 + CviJI RGCY 3869 3869 . . 3868 3871 + HaeIII GGCC 3869 3869 . . 3877 3881 + Hpy188I TCNGA 3879 3878 . . 3881 3884 + TspEI AATT 3880 3884 . . 3886 3890 + TspDTI ATGAA 3901 3899 . . 3888 3893 + ApoI RAATTY 3888 3892 . . 3889 3892 + TspEI AATT 3888 3892 . . 3907 3912 + XhoII RGATCY 3907 3911 . . 3907 3912 + NlaIV GGNNCC 3909 3909 . . 3907 3912 + BamHI GGATCC 3907 3911 . . 3907 3911 + BinI* GGATC 3915 3916 . . 3908 3911 + DpnI GATC 3909 3909 . . 3908 3911 + BstKTI GATC 3910 3908 . . 3908 3911 + MboI GATC 3907 3911 . . 3904 3908 - BccI CCATC 3898 3899 . . 3908 3912 - BinI* GGATC 3902 3903 . . 3946 3949 + MseI TTAA 3946 3948 . . 3953 3957 + TfiI GAWTC 3953 3956 . . 3953 3957 + HinfI GANTC 3953 3956 . . 3956 3959 + TaqI TCGA 3956 3958 . . 3959 3963 + TspDTI ATGAA 3974 3972 . . 3962 3965 + TspEI AATT 3961 3965 . . 3964 3967 + MseI TTAA 3964 3966 . . 3981 3984 + CviJI RGCY 3982 3982 . . 3990 3994 + HgaI GACGC 3999 4004 . . 3990 3995 + AcyI GRCGYC 3991 3993 . . 3994 3997 + HpaII CCGG 3994 3996 . . 4011 4015 + BlsI GCNGC 4013 4012 . . 4011 4015 + BisI GCNGC 4012 4013 . . 4011 4015 + BbvI GCAGC 4023 4027 . . 4011 4015 + TseI GCWGC 4011 4014 . . 4012 4017 + PvuII CAGCTG 4014 4014 . . 4012 4017 + NspBII* CMGCKG 4014 4014 . . 4013 4016 + SetI ASST 4016 4012 . . 4013 4016 + CviJI RGCY 4014 4014 . . 4013 4016 + AluI AGCT 4014 4014 . . 4014 4018 + BlsI GCNGC 4016 4015 . . 4014 4018 + BisI GCNGC 4015 4016 . . 4014 4018 + TseI GCWGC 4014 4017 . . 4018 4022 + BssKI CCNGG 4017 4022 . . 4018 4022 + BseBI CCWGG 4019 4020 . . 4018 4022 + ScrFI CCNGG 4019 4020 . . 4014 4018 - BbvI GCAGC 4001 4005 . . 4018 4022 + EcoRII CCWGG 4017 4022 . . 4030 4034 - Hin4II* CCTTC 4024 4023 . . 4035 4039 + BsrI ACTGG 4040 4038 . . 4038 4041 + CviJI RGCY 4039 4039 . . 4038 4043 + NlaIV GGNNCC 4040 4040 . . 4046 4050 + Hpy188I TCNGA 4048 4047 . . 4049 4052 + DpnI GATC 4050 4050 . . 4049 4052 + BstKTI GATC 4051 4049 . . 4049 4052 + MboI GATC 4048 4052 . . 4074 4078 + HphI GGTGA 4086 4085 . . 4077 4081 + MboII GAAGA 4089 4088 . . 4077 4082 + BbvII* GAAGAC 4084 4088 . . 4080 4089 + PshAI GACNNNNGTC 4084 4084 . . 4084 4087 + MaeI CTAG 4084 4086 . . 4087 4091 + Tsp45I GTSAC 4086 4091 . . 4087 4091 + MaeIII GTNAC 4086 4091 . . 4090 4094 + Tsp4CI* ACNGT 4092 4091 . . 4092 4097 + TatI WGTACW 4092 4096 . . 4092 4097 + ScaI AGTACT 4094 4094 . . 4093 4096 + Csp6I GTAC 4093 4095 . . 4093 4096 + RsaI GTAC 4094 4094 . . 4102 4106 + Hpy188I TCNGA 4104 4103 . . 4113 4123 + MwoI GCNNNNNNNGC 4119 4116 . . 4117 4121 - Hin4II* CCTTC 4111 4110 . . 4134 4138 + PleI GAGTC 4142 4143 . . 4134 4138 + MlyI GAGTC 4143 4143 . . 4130 4134 - Hin4II* CCTTC 4124 4123 . . 4134 4138 + HinfI GANTC 4134 4137 . . 4137 4141 + Hpy188I TCNGA 4139 4138 . . 4151 4156 + BsrDI GCAATG 4158 4156 . . 4160 4165 + Hpy178III* TCNNGA 4161 4163 . . 4157 4161 - MboII GAAGA 4149 4148 . . 4156 4161 - BbvII* GAAGAC 4149 4153 . . 4177 4187 - BsaXI ACNNNNNCTCC 4199 4196 4169 4166 4177 4188 - TstI CACNNNNNNTCC 4201 4196 4169 4164 4190 4193 + TspEI AATT 4189 4193 . . 4199 4202 + MseI TTAA 4199 4201 . . 4202 4205 + TspEI AATT 4201 4205 . . 4204 4207 + MseI TTAA 4204 4206 . . 4208 4213 - Eco57MI CTGRAG 4193 4191 . . 4213 4217 + BslFI GGGAC 4227 4231 . . 4208 4213 - Eco57I CTGAAG 4193 4191 . . 4238 4243 + SecI* CCNNGG 4238 4242 . . 4240 4243 - MnlI CCTC 4233 4232 . . 4261 4266 + NlaIV GGNNCC 4263 4263 . . 4265 4275 + BsiYI* CCNNNNNNNGG 4271 4268 . . 4276 4281 + Hpy166II GTNNAC 4278 4278 . . 4278 4281 + Csp6I GTAC 4278 4280 . . 4278 4281 + RsaI GTAC 4279 4279 . . 4283 4288 + EcoT22I ATGCAT 4287 4283 . . 4284 4287 + CviRI* TGCA 4285 4285 . . 4285 4289 + SfaNI GCATC 4294 4298 . . 4282 4286 - SfaNI GCATC 4272 4276 . . 4288 4291 + TaqI TCGA 4288 4290 . . 4291 4296 + ApoI RAATTY 4291 4295 . . 4292 4295 + TspEI AATT 4291 4295 . . 4297 4300 + CviJI RGCY 4298 4298 . . 4297 4302 + NlaIV GGNNCC 4299 4299 . . 4301 4306 + NspBII* CMGCKG 4303 4303 . . 4301 4304 + AciI CCGC 4301 4303 . . 4303 4313 + MwoI GCNNNNNNNGC 4309 4306 . . 4310 4315 + BsgI GTGCAG 4331 4329 . . 4306 4310 - BccI CCATC 4300 4301 . . 4311 4314 + CviRI* TGCA 4312 4312 . . 4316 4320 + Tsp4CI* ACNGT 4318 4317 . . 4317 4323 + TspRI CASTGNN 4323 4314 . . 4325 4330 + PstI CTGCAG 4329 4325 . . 4325 4330 + SfeI* CTRYAG 4325 4329 . . 4326 4329 + CviRI* TGCA 4327 4327 . . 4327 4331 + BlsI GCNGC 4329 4328 . . 4327 4331 + BisI GCNGC 4328 4329 . . 4327 4331 + BbvI GCAGC 4339 4343 . . 4327 4331 + TseI GCWGC 4327 4330 . . 4328 4333 + PvuII CAGCTG 4330 4330 . . 4328 4333 + NspBII* CMGCKG 4330 4330 . . 4329 4332 + SetI ASST 4332 4328 . . 4329 4332 + CviJI RGCY 4330 4330 . . 4329 4332 + AluI AGCT 4330 4330 . . 4344 4349 - BciVI GTATCC 4338 4337 . . 4355 4358 + TaqI TCGA 4355 4357 . . 4357 4362 + BseRI GAGGAG 4372 4370 . . 4357 4360 - MnlI CCTC 4350 4349 . . 4362 4366 + BinI* GGATC 4370 4371 . . 4360 4363 - MnlI CCTC 4353 4352 . . 4363 4366 + DpnI GATC 4364 4364 . . 4363 4366 + BstKTI GATC 4365 4363 . . 4363 4366 + MboI GATC 4362 4366 . . 4359 4369 - BsaXI ACNNNNNCTCC 4381 4378 4351 4348 4382 4387 + SspI AATATT 4384 4384 . . 4387 4393 + PfoI TCCNGGA 4387 4392 . . 4388 4392 + BssKI CCNGG 4387 4392 . . 4388 4392 + BseBI CCWGG 4389 4390 . . 4388 4392 + ScrFI CCNGG 4389 4390 . . 4388 4392 + EcoRII CCWGG 4387 4392 . . 4392 4402 + Hin4I GAYNNNNNVTC 4383 4378 4415 4410 4399 4402 + DpnI GATC 4400 4400 . . 4399 4402 + BstKTI GATC 4401 4399 . . 4399 4402 + MboI GATC 4398 4402 . . 4401 4406 + Hpy178III* TCNNGA 4402 4404 . . 4401 4406 + BspHI TCATGA 4401 4405 . . 4392 4402 - Hin4I GAYNNNNNVTC 4415 4410 4383 4378 4402 4405 + FatI CATG 4401 4405 . . 4402 4405 + NlaIII CATG 4405 4401 . . 4402 4405 + CviAII CATG 4402 4404 . . 4405 4410 + SduI GDGCHC 4409 4405 . . 4405 4410 + HgiJII* GRGCYC 4409 4405 . . 4406 4409 + CviJI RGCY 4407 4407 . . 4409 4414 + DsaI* CCRYGG 4409 4413 . . 4409 4414 + SecI* CCNNGG 4409 4413 . . 4411 4415 + BceAI ACGGC 4427 4429 . . 4422 4431 + MslI CAYNNNNRTG 4426 4426 . . 4423 4428 + ClaI ATCGAT 4424 4426 . . 4424 4427 + TaqI TCGA 4424 4426 . . 4432 4436 - MboII GAAGA 4424 4423 . . 4431 4436 - BbvII* GAAGAC 4424 4428 . . 4438 4442 + BssKI CCNGG 4437 4442 . . 4438 4441 + HpaII CCGG 4438 4440 . . 4438 4442 + ScrFI CCNGG 4439 4440 . . 4438 4442 + CauII* CCSGG 4439 4440 . . 4435 4439 - HphI GGTGA 4427 4426 . . 4444 4447 + DpnI GATC 4445 4445 . . 4444 4447 + BstKTI GATC 4446 4444 . . 4444 4447 + MboI GATC 4443 4447 . . 4458 4467 + MslI CAYNNNNRTG 4462 4462 . . 4458 4467 + OliI CACNNNNGTG 4462 4462 . . 4467 4471 + PleI GAGTC 4475 4476 . . 4467 4471 + MlyI GAGTC 4476 4476 . . 4467 4471 + HinfI GANTC 4467 4470 . . 4473 4476 + Csp6I GTAC 4473 4475 . . 4473 4476 + RsaI GTAC 4474 4474 . . 4480 4483 + DpnI GATC 4481 4481 . . 4480 4483 + BstKTI GATC 4482 4480 . . 4480 4483 + MboI GATC 4479 4483 . . 4484 4487 + CviJI RGCY 4485 4485 . . 4488 4491 + MnlI CCTC 4498 4497 . . 4490 4493 + TaqI TCGA 4490 4492 . . 4493 4496 + SetI ASST 4496 4492 . . 4493 4498 + BspMI ACCTGC 4502 4506 . . 4494 4504 + BsiYI* CCNNNNNNNGG 4500 4497 . . 4499 4504 + DsaI* CCRYGG 4499 4503 . . 4499 4504 + SecI* CCNNGG 4499 4503 . . 4509 4513 - Hin4II* CCTTC 4503 4502 . . 4513 4516 - MnlI CCTC 4506 4505 . . 4524 4528 - TspDTI ATGAA 4514 4512 . . 4537 4542 + NdeI CATATG 4538 4540 . . 4540 4545 + EcoT22I ATGCAT 4544 4540 . . 4541 4544 + CviRI* TGCA 4542 4542 . . 4549 4559 + MwoI GCNNNNNNNGC 4555 4552 . . 4550 4555 + SplI* CGTACG 4550 4554 . . 4551 4554 + Csp6I GTAC 4551 4553 . . 4551 4554 + RsaI GTAC 4552 4552 . . 4555 4559 - SfaNI GCATC 4545 4549 . . 4558 4561 - AciI CCGC 4558 4560 . . 4566 4569 - MnlI CCTC 4559 4558 . . 4575 4579 + BslFI GGGAC 4589 4593 . . 4582 4586 + TspDTI ATGAA 4597 4595 . . 4587 4591 + FokI GGATG 4600 4604 . . 4587 4591 + BseGI GGATG 4593 4591 . . 4584 4588 - Hin4II* CCTTC 4578 4577 . . 4603 4607 + BmgT120I GGNCC 4604 4605 . . 4603 4607 + AsuI* GGNCC 4603 4606 . . 4601 4604 - MnlI CCTC 4594 4593 . . 4604 4607 + CviJI RGCY 4605 4605 . . 4604 4607 + HaeIII GGCC 4605 4605 . . 4605 4610 + NmeAIII GCCGAG 4631 4629 . . 4609 4614 + HaeII RGCGCY 4613 4609 . . 4610 4613 + Hin6I GCGC 4610 4612 . . 4610 4613 + GlaI GCGC 4611 4611 . . 4610 4613 + HhaI GCGC 4612 4610 . . 4612 4616 + TauI GCSGC 4615 4612 . . 4612 4616 + BlsI GCNGC 4614 4613 . . 4612 4616 + BisI GCNGC 4613 4614 . . 4613 4616 + AciI CCGC 4613 4615 . . 4615 4619 + BlsI GCNGC 4617 4616 . . 4615 4619 + BisI GCNGC 4616 4617 . . 4615 4619 + BbvI GCAGC 4627 4631 . . 4615 4619 + TseI GCWGC 4615 4618 . . 4616 4621 + EcoP15I CAGCAG 4646 4648 . . 4630 4640 + MwoI GCNNNNNNNGC 4636 4633 . . 4631 4634 + MaeI CTAG 4631 4633 . . 4635 4638 + SetI ASST 4638 4634 . . 4635 4638 + CviJI RGCY 4636 4636 . . 4635 4638 + AluI AGCT 4636 4636 . . 4636 4640 + BlsI GCNGC 4638 4637 . . 4636 4640 + BisI GCNGC 4637 4638 . . 4636 4640 + TseI GCWGC 4636 4639 . . 4638 4641 + CviRI* TGCA 4639 4639 . . 4639 4649 + MwoI GCNNNNNNNGC 4645 4642 . . 4636 4640 - BbvI GCAGC 4623 4627 . . 4641 4644 + SetI ASST 4644 4640 . . 4637 4642 - BsgI GTGCAG 4622 4620 . . 4650 4653 + TspEI AATT 4649 4653 . . 4657 4662 + McrI* CGRYCG 4660 4658 . . 4657 4662 + XmaIII* CGGCCG 4657 4661 . . 4657 4662 + CfrI YGGCCR 4657 4661 . . 4658 4661 + CviJI RGCY 4659 4659 . . 4658 4661 + HaeIII GGCC 4659 4659 . . 4660 4670 + BsiYI* CCNNNNNNNGG 4666 4663 . . 4663 4666 + MseI TTAA 4663 4665 . . 4659 4663 - BceAI ACGGC 4644 4646 . . 4665 4668 + TspEI AATT 4664 4668 . . 4669 4674 + NlaIV GGNNCC 4671 4671 . . 4670 4674 + BmgT120I GGNCC 4671 4672 . . 4670 4674 + AsuI* GGNCC 4670 4673 . . 4671 4674 + CviJI RGCY 4672 4672 . . 4671 4674 + HaeIII GGCC 4672 4672 . . 4672 4682 + MwoI GCNNNNNNNGC 4678 4675 . . 4672 4682 + BglI GCCNNNNNGGC 4678 4675 . . 4674 4680 + TspRI CASTGNN 4680 4671 . . 4676 4680 + DdeI CTNAG 4676 4679 . . 4676 4680 - BspCNI CTCAG 4668 4666 . . 4676 4680 - BseMII CTCAG 4667 4665 . . 4678 4681 - MnlI CCTC 4671 4670 . . 4681 4686 + BtsI GCAGTG 4688 4686 . . 4682 4688 + TspRI CASTGNN 4688 4679 . . 4678 4688 - Hin4I GAYNNNNNVTC 4701 4696 4669 4664 4688 4692 + DdeI CTNAG 4688 4691 . . 4707 4710 + SetI ASST 4710 4706 . . 4707 4710 + CviJI RGCY 4708 4708 . . 4707 4710 + AluI AGCT 4708 4708 . . 4725 4729 + HgaI GACGC 4734 4739 . . 4728 4738 + MwoI GCNNNNNNNGC 4734 4731 . . 4751 4756 + TatI WGTACW 4751 4755 . . 4752 4755 + Csp6I GTAC 4752 4754 . . 4752 4755 + RsaI GTAC 4753 4753 . . 4764 4768 + Hpy188I TCNGA 4766 4765 . . 4771 4775 + TfiI GAWTC 4771 4774 . . 4771 4775 + HinfI GANTC 4771 4774 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 1284 1589 1906 # # AciI: # [CCGC] # 31 33 52 55 59 166 # 197 225 231 257 448 462 # 467 654 1442 # # AcyI: # [GRCGYC] # 788 3991 # # AflII: # [CTTAAG] # 1507 3272 # # AflIII: # [ACRYGT] # 1368 3411 # # AhaIII*: # [TTTAAA] # 192 219 1121 1188 2059 # # AloI: # [GAACNNNNNNTCC] # 32 1648 3099 # # AluI: # [AGCT] # 41 53 70 71 72 153 # 160 203 204 207 240 246 # 306 316 400 483 729 825 # # AlwNI: # [CAGNNNCTG] # 226 4553 # # ApaLI: # [GTGCAC] # 1617 3162 # # ApoI: # [RAATTY] # 8 34 42 51 59 66 # 95 148 175 177 182 283 # 297 399 403 488 1872 # # AsuI*: # [GGNCC] # 67 109 258 279 501 521 # 736 968 1340 # # AsuII: # [TTCGAA] # 1565 3214 # # AvaII: # [GGWCC] # 1170 1469 2140 # # BalI: # [TGGCCA] # 1197 3582 # # BamHI: # [GGATCC] # 872 3907 # # BbvI: # [GCAGC] # 4 22 23 152 284 316 # 3978 # # BbvII*: # [GAAGAC] # 65 275 355 818 3266 # # BccI: # [CCATC] # 186 238 238 402 479 498 # 594 878 1266 # # Bce83I*: # [CTTGAG] # 985 1861 1933 # # BceAI: # [ACGGC] # 135 217 4427 # # BcgI: # [CGANNNNNNTGC] # 34 1551 3194 # # BciVI: # [GTATCC] # 441 559 1452 2327 # # BfiI: # [ACTGGG] # 812 875 3092 # # BglI: # [GCCNNNNNGGC] # 101 4678 # # BglII: # [AGATCT] # 333 4446 # # BinI*: # [GGATC] # 13 198 213 358 392 409 # 455 2741 # # BisI: # [GCNGC] # 3 3 3 21 34 142 # 285 313 424 1532 2019 # # BlsI: # [GCNGC] # 3 3 3 21 35 141 # 285 313 424 1532 2019 # # BmgT120I: # [GGNCC] # 67 108 258 279 502 521 # 736 968 1340 # # BplI: # [GAGNNNNNCTC] # 0 0 32 1305 3442 # # Bpu10I: # [CCTNAGC] # 516 4263 # # BsaAI: # [YACGTR] # 1086 3693 # # BsaBI: # [GATNNNNATC] # 140 300 435 963 1012 1929 # # BsaXI: # [ACNNNNNCTCC] # 30 30 152 398 4169 # # BseBI: # [CCWGG] # 70 221 370 371 390 406 # 438 465 976 1072 # # BseGI: # [GGATG] # 84 186 250 255 326 388 # 389 610 773 1518 # # BseMII: # [CTCAG] # 112 130 158 173 320 625 # 1463 1798 # # BseRI: # [GAGGAG] # 407 4372 # # BseSI: # [GKGCMC] # 1013 1621 2145 # # BsgI: # [GTGCAG] # 157 291 1966 2365 # # BsiI*: # [CACGAG] # 1883 2896 # # BsiYI*: # [CCNNNNNNNGG] # 113 166 188 229 305 336 # 409 456 578 1999 # # BslFI: # [GGGAC] # 190 362 4227 # # BsmAI: # [GTCTC] # 1425 3354 # # Bsp1407I: # [TGTACA] # 266 1574 2939 # # BspCNI: # [CTCAG] # 111 130 158 173 319 625 # 1465 1798 # # BspHI: # [TCATGA] # 266 378 1090 3045 # # BspLU11I*: # [ACATGT] # 1368 3411 # # BspMI: # [ACCTGC] # 277 4502 # # BsrDI: # [GCAATG] # 82 621 1282 2794 # # BsrI: # [ACTGG] # 103 157 284 386 478 534 # 600 640 739 858 # # BssKI: # [CCNGG] # 50 70 74 221 342 364 # 370 371 406 465 484 492 # 1070 # # BstEII: # [GGTNACC] # 11 4768 # # BstKTI: # [GATC] # 30 36 36 45 45 63 # 67 141 150 156 159 166 # 177 195 297 312 314 512 # 722 1156 # # BtgZI: # [GCGATG] # 1299 3480 # # BtrI: # [CACGTC] # 1243 3536 # # BtsI: # [GCAGTG] # 91 1035 1499 2154 # # Cac8I: # [GCNNGC] # 437 748 1135 2459 # # CauII*: # [CCSGG] # 340 558 1609 2272 # # CfrI: # [YGGCCR] # 45 122 1032 3580 # # ClaI: # [ATCGAT] # 18 242 355 723 903 2538 # # Csp6I: # [GTAC] # 27 53 64 78 101 183 # 185 195 201 202 252 270 # 310 387 684 777 810 # # CviAII: # [CATG] # 133 134 165 233 248 372 # 377 417 525 565 742 868 # # CviJI: # [RGCY] # 12 12 13 13 23 25 # 27 31 32 32 35 36 # 36 36 41 42 43 43 # 45 48 53 71 75 75 # 77 78 86 113 117 120 # 123 124 132 153 155 160 # 165 168 168 180 194 199 # 207 259 284 303 315 # # CviRI*: # [TGCA] # 15 21 27 43 70 97 # 140 146 146 149 177 199 # 215 284 358 537 622 756 # 777 # # DdeI: # [CTNAG] # 12 91 113 125 130 158 # 173 184 193 210 221 355 # 415 415 947 1037 # # DpnI: # [GATC] # 30 36 36 45 45 63 # 67 141 150 156 158 166 # 177 195 298 312 314 512 # 722 1156 # # DraII: # [RGGNCCY] # 501 4278 # # DsaI*: # [CCRYGG] # 90 280 791 832 2786 # # Eam1105I: # [GACNNNNNGTC] # 735 4044 # # Ecl136II: # [GAGCTC] # 1590 3189 # # Eco47III: # [AGCGCT] # 1709 3070 # # Eco57I: # [CTGAAG] # 37 358 586 1875 1923 # # Eco57MI: # [CTGRAG] # 37 358 586 918 1005 1875 # # EcoP15I: # [CAGCAG] # 133 4646 # # EcoRI: # [GAATTC] # 342 1446 2991 # # EcoRII: # [CCWGG] # 70 221 370 371 392 406 # 438 465 976 1070 # # EcoRV: # [GATATC] # 33 102 1029 3615 # # EcoT22I: # [ATGCAT] # 235 257 4287 # # EspI*: # [GCTNAGC] # 1762 3017 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 1050 1569 2096 # # FatI: # [CATG] # 132 134 165 233 248 372 # 378 417 525 565 742 868 # # FauI: # [CCCGC] # 933 3846 # # FnuDII*: # [CGCG] # 467 1632 2680 # # FokI: # [GGATG] # 84 179 270 275 313 388 # 389 610 753 1518 # # GlaI: # [GCGC] # 168 339 581 789 934 970 # 998 # # GsuI: # [CTGGAG] # 1313 3466 # # HaeII: # [RGCGCY] # 166 791 920 2902 # # HaeIII: # [GGCC] # 13 45 54 107 242 271 # 278 502 655 736 835 1041 # # HgaI: # [GACGC] # 45 643 735 3356 # # HgiAI*: # [GWGCWC] # 1570 1588 1621 # # HgiCI*: # [GGYRCC] # 282 1868 2629 # # HgiJII*: # [GRGCYC] # 370 1218 3191 # # HhaI: # [GCGC] # 167 339 581 790 934 970 # 998 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 5 27 # 27 32 32 32 32 32 # 32 32 70 78 254 271 # 312 391 675 1063 1382 # # Hin4II*: # [CCTTC] # 13 32 61 62 75 87 # 120 201 317 327 379 409 # 460 507 516 555 658 # # Hin6I: # [GCGC] # 169 339 581 788 934 970 # 998 # # HindII: # [GTYRAC] # 198 1956 2625 # # HindIII: # [AAGCTT] # 153 446 613 1252 2315 # # HinfI: # [GANTC] # 8 58 93 135 140 141 # 153 165 174 176 181 207 # 243 264 304 307 333 502 # 562 633 # # HpaI: # [GTTAAC] # 2154 2625 # # HpaII: # [CCGG] # 341 370 444 557 795 2272 # # HphI: # [GGTGA] # 341 352 422 453 660 761 # 1790 # # Hpy166II: # [GTNNAC] # 29 49 198 237 298 501 # 616 625 720 739 767 # # Hpy178III*: # [TCNNGA] # 12 26 44 67 72 93 # 116 144 181 241 267 296 # 300 300 340 354 377 378 # 386 389 396 # # Hpy188I: # [TCNGA] # 13 13 35 37 56 89 # 95 105 107 115 117 118 # 134 169 171 283 309 349 # 412 433 487 505 627 # # Hpy99I: # [CGWCG] # 1336 3443 # # Ksp632I*: # [CTCTTC] # 71 306 383 414 1320 2285 # # MaeI: # [CTAG] # 148 464 547 607 732 1077 # 1204 # # MaeII: # [ACGT] # 10 47 51 75 157 372 # 801 1037 1087 1142 # # MaeIII: # [GTNAC] # 11 30 94 105 191 196 # 204 204 270 531 693 2250 # # MboI: # [GATC] # 30 36 36 45 45 63 # 67 141 150 156 156 166 # 177 195 300 312 314 512 # 722 1156 # # MboII: # [GAAGA] # 3 5 15 30 30 60 # 66 79 86 94 124 160 # 173 268 275 336 355 401 # 494 559 580 586 # # McrI*: # [CGRYCG] # 4 119 1028 3628 # # MfeI: # [CAATTG] # 1382 3397 # # MlyI: # [GAGTC] # 153 213 243 292 303 333 # 492 563 683 697 807 # # MnlI: # [CCTC] # 1 3 8 26 30 35 # 40 53 72 77 90 96 # 108 117 138 145 171 179 # 185 201 557 565 880 1002 # # MseI: # [TTAA] # 5 5 6 13 18 31 # 32 39 40 42 44 55 # 58 64 71 73 87 90 # 90 108 111 116 129 134 # 144 156 185 186 211 216 # 235 242 308 336 459 640 # # MslI: # [CAYNNNNRTG] # 36 317 615 1046 1185 1580 # # MstI*: # [TGCGCA] # 1370 3409 # # MwoI: # [GCNNNNNNNGC] # 9 33 45 56 63 81 # 190 246 598 798 1004 1656 # # NcoI: # [CCATGG] # 791 3988 # # NdeI: # [CATATG] # 241 1257 1595 1686 # # NlaIII: # [CATG] # 134 136 165 233 248 372 # 374 417 525 565 742 868 # # NlaIV: # [GGNNCC] # 36 108 131 223 282 372 # 762 763 996 1106 # # NmeAIII: # [GCCGAG] # 148 4631 # # NspBII*: # [CMGCKG] # 27 289 449 4014 # # NspI: # [RCATGY] # 1364 3415 # # OliI: # [CACNNNNGTG] # 317 4462 # # PasI: # [CCCWGGG] # 2345 2434 # # PfoI: # [TCCNGGA] # 392 1559 2828 # # PleI: # [GAGTC] # 153 212 243 293 304 333 # 492 562 683 697 807 # # PmaCI: # [CACGTG] # 1086 3693 # # PshAI: # [GACNNNNGTC] # 695 4084 # # PsiI: # [TTATAA] # 718 949 3112 # # PsrI: # [GAACNNNNNNTAC] # 32 2156 2591 # # PstI: # [CTGCAG] # 450 4329 # # PvuI: # [CGATCG] # 1147 3632 # # PvuII: # [CAGCTG] # 316 449 4014 # # RsaI: # [GTAC] # 26 53 64 78 101 183 # 185 195 201 202 252 270 # 310 388 684 777 810 # # SacI: # [GAGCTC] # 1588 3191 # # SapI: # [GCTCTTC] # 377 383 414 3605 # # ScaI: # [AGTACT] # 685 1895 2199 # # ScrFI: # [CCNGG] # 50 70 74 221 340 364 # 370 371 406 465 484 492 # 1072 # # SduI: # [GDGCHC] # 370 557 1013 1218 1621 # # SecI*: # [CCNNGG] # 1 90 171 256 280 661 # 791 976 1553 # # SetI: # [ASST] # 6 6 9 10 14 16 # 18 21 21 29 30 32 # 32 32 39 41 44 45 # 51 55 59 66 69 70 # 70 70 75 76 79 81 # 94 98 106 112 134 142 # 153 160 164 166 175 181 # 203 207 212 289 300 301 # 316 # # SfaNI: # [GCATC] # 22 32 108 234 251 284 # 315 335 388 2810 # # SfeI*: # [CTRYAG] # 104 454 525 3696 # # SmlI: # [CTYRAG] # 441 985 1507 1846 # # SplI*: # [CGTACG] # 229 4550 # # SspI: # [AATATT] # 75 137 395 608 945 1138 # 1481 # # StyI: # [CCWWGG] # 791 3988 # # TaiI: # [ACGT] # 10 50 51 75 157 372 # 801 1037 1084 1142 # # TaqI: # [TCGA] # 15 18 24 66 67 69 # 87 89 92 104 111 133 # 213 218 242 257 289 324 # 332 390 646 993 # # TaqII: # [GACCGA] # 909 3870 # # TatI: # [WGTACW] # 28 53 64 202 252 270 # 659 684 993 1574 # # TauI: # [GCSGC] # 36 164 427 1532 2620 # # TfiI: # [GAWTC] # 8 135 141 207 560 710 # 726 727 747 818 # # TseI: # [GCWGC] # 3 21 36 143 288 313 # 3975 # # TsoI: # [TARCCA] # 307 950 975 2547 # # Tsp45I: # [GTSAC] # 30 116 591 693 801 2548 # # Tsp4CI*: # [ACNGT] # 11 78 94 196 212 222 # 226 267 268 303 410 461 # 503 752 776 # # TspDTI: # [ATGAA] # 30 43 73 83 112 116 # 126 132 134 134 145 154 # 182 186 225 232 305 337 # 446 495 540 549 # # TspEI: # [AATT] # 4 5 7 8 8 9 # 11 12 12 13 14 15 # 17 17 20 20 27 29 # 32 34 35 35 36 36 # 37 39 39 43 44 51 # 59 62 66 68 71 73 # 90 90 98 115 116 119 # 128 136 148 155 168 182 # 186 228 249 318 337 358 # 450 # # TspGWI: # [ACGGA] # 225 1834 2720 # # TspRI: # [CASTGNN] # 8 91 203 291 340 357 # 471 534 670 786 1028 # # TstI: # [CACNNNNNNTCC] # 32 578 4169 # # Tth111I: # [GACNNNGTC] # 465 1635 2679 # # VspI: # [ATTAAT] # 1022 3757 # # XbaI: # [TCTAGA] # 606 1773 2400 # # XhoII: # [RGATCY] # 243 333 590 872 2741 # # XmaIII*: # [CGGCCG] # 122 1032 3625 # # XmnI: # [GAANNNNTTC] # 1442 3337 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4779 # Reported_sequences: 1 # Reported_hitcount: 1125 #---------------------------------------