Restriction Fragments for NNR1/YNL200C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

NNR1/YNL200C on chromosome XIV from coordinates 264453 to 263713.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20872.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 3 8 + AccI GTMKAC 4 6 . . 7 10 + TaiI ACGT 10 6 . . 7 10 + SetI ASST 10 6 . . 7 10 + MaeII ACGT 7 9 . . 21 25 - TspDTI ATGAA 11 9 . . 28 33 + HindIII AAGCTT 28 32 . . 29 32 + SetI ASST 32 28 . . 29 32 + CviJI RGCY 30 30 . . 29 32 + AluI AGCT 30 30 . . 30 35 + Cac8I GCNNGC 32 32 . . 33 36 + CviRI* TGCA 34 34 . . 34 38 + BlsI GCNGC 36 35 . . 34 38 + BisI GCNGC 35 36 . . 34 38 + BbvI GCAGC 46 50 . . 34 38 + TseI GCWGC 34 37 . . 35 40 + PvuII CAGCTG 37 37 . . 35 40 + NspBII* CMGCKG 37 37 . . 36 39 + SetI ASST 39 35 . . 36 39 + CviJI RGCY 37 37 . . 36 39 + AluI AGCT 37 37 . . 44 47 + TaqI TCGA 44 46 . . 53 56 + TspEI AATT 52 56 . . 57 67 + Hin4I GAYNNNNNVTC 48 43 80 75 60 66 + PpuMI RGGWCCY 61 64 . . 60 66 + DraII RGGNCCY 61 64 . . 60 65 + NlaIV GGNNCC 62 62 . . 61 65 + BmgT120I GGNCC 62 63 . . 57 61 - BccI CCATC 51 52 . . 61 65 + AvaII GGWCC 61 64 . . 61 65 + AsuI* GGNCC 61 64 . . 64 67 + MnlI CCTC 74 73 . . 64 74 + BsiYI* CCNNNNNNNGG 70 67 . . 71 75 + Hpy188I TCNGA 73 72 . . 74 78 + TfiI GAWTC 74 77 . . 74 78 + HinfI GANTC 74 77 . . 89 92 + SetI ASST 92 88 . . 89 92 + CviJI RGCY 90 90 . . 89 92 + AluI AGCT 90 90 . . 102 105 + SetI ASST 105 101 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + AluI AGCT 103 103 . . 103 108 - GsaI CCCAGC 107 103 . . 103 108 - BseYI CCCAGC 103 107 . . 117 120 + FnuDII* CGCG 118 118 . . 118 121 + Hin6I GCGC 118 120 . . 118 121 + GlaI GCGC 119 119 . . 118 121 + HhaI GCGC 120 118 . . 120 125 + Cac8I GCNNGC 122 122 . . 123 126 + CviJI RGCY 124 124 . . 135 139 - BsrI ACTGG 135 133 . . 142 152 + BsiYI* CCNNNNNNNGG 148 145 . . 143 149 + TspRI CASTGNN 149 140 . . 145 149 + DdeI CTNAG 145 148 . . 145 149 - BspCNI CTCAG 137 135 . . 145 149 - BseMII CTCAG 136 134 . . 149 152 - MnlI CCTC 142 141 . . 163 167 + TspGWI ACGGA 178 176 . . 177 182 + BspLU11I* ACATGT 177 181 . . 177 182 + NspI RCATGY 181 177 . . 177 182 + AflIII ACRYGT 177 181 . . 178 181 + FatI CATG 177 181 . . 178 181 + NlaIII CATG 181 177 . . 178 181 + CviAII CATG 178 180 . . 196 200 + BmgT120I GGNCC 197 198 . . 196 200 + AsuI* GGNCC 196 199 . . 197 200 + CviJI RGCY 198 198 . . 197 200 + HaeIII GGCC 198 198 . . 193 198 - GsaI CCCAGC 197 193 . . 193 198 - BseYI CCCAGC 193 197 . . 199 203 + BssKI CCNGG 198 203 . . 199 203 + BseBI CCWGG 200 201 . . 199 203 + ScrFI CCNGG 200 201 . . 199 203 + EcoRII CCWGG 198 203 . . 201 204 + SetI ASST 204 200 . . 203 207 + MaeIII GTNAC 202 207 . . 212 215 - AciI CCGC 212 214 . . 216 220 + FokI GGATG 229 233 . . 212 216 - FauI CCCGC 205 207 . . 216 220 + BseGI GGATG 222 220 . . 220 225 + Eco31I GGTCTC 226 230 . . 217 221 - BccI CCATC 211 212 . . 221 225 + BsmAI GTCTC 226 230 . . 223 228 - BsiI* CACGAG 223 227 . . 229 234 + MstI* TGCGCA 231 231 . . 230 233 + Hin6I GCGC 230 232 . . 230 233 + GlaI GCGC 231 231 . . 230 233 + HhaI GCGC 232 230 . . 244 249 + HindIII AAGCTT 244 248 . . 245 248 + SetI ASST 248 244 . . 245 248 + CviJI RGCY 246 246 . . 245 248 + AluI AGCT 246 246 . . 254 258 + MaeIII GTNAC 253 258 . . 252 257 - TsoI TARCCA 242 240 . . 287 292 + Cac8I GCNNGC 289 289 . . 291 294 + Hin6I GCGC 291 293 . . 291 294 + GlaI GCGC 292 292 . . 291 294 + HhaI GCGC 293 291 . . 294 300 + TspRI CASTGNN 300 291 . . 298 303 + EcoRI GAATTC 298 302 . . 298 303 + ApoI RAATTY 298 302 . . 299 302 + TspEI AATT 298 302 . . 310 315 + PvuII CAGCTG 312 312 . . 310 315 + NspBII* CMGCKG 312 312 . . 311 314 + SetI ASST 314 310 . . 311 314 + CviJI RGCY 312 312 . . 311 314 + AluI AGCT 312 312 . . 316 321 + Hpy166II GTNNAC 318 318 . . 322 327 + MfeI CAATTG 322 326 . . 318 322 - HphI GGTGA 310 309 . . 323 326 + TspEI AATT 322 326 . . 326 337 + BdaI TGANNNNNNTCA 315 313 349 347 327 336 + XmnI GAANNNNTTC 331 331 . . 327 332 + ApoI RAATTY 327 331 . . 328 331 + TspEI AATT 327 331 . . 326 337 - BdaI TGANNNNNNTCA 349 347 315 313 351 355 + BsmAI GTCTC 356 360 . . 353 358 + SmlI CTYRAG 353 357 . . 354 359 + Hpy178III* TCNNGA 355 357 . . 353 358 - Bce83I* CTTGAG 338 336 . . 361 364 - MnlI CCTC 354 353 . . 368 372 + BsrI ACTGG 373 371 . . 371 374 + CviJI RGCY 372 372 . . 377 382 + SspI AATATT 379 379 . . 386 389 + CviJI RGCY 387 387 . . 388 391 + HpaII CCGG 388 390 . . 397 400 + SetI ASST 400 396 . . 402 407 + EcoT22I ATGCAT 406 402 . . 403 406 + CviRI* TGCA 404 404 . . 414 417 + CviRI* TGCA 415 415 . . 412 416 - SfaNI GCATC 402 406 . . 417 422 + SspI AATATT 419 419 . . 437 440 + CviJI RGCY 438 438 . . 439 442 + MnlI CCTC 449 448 . . 444 447 + FatI CATG 443 447 . . 444 447 + NlaIII CATG 447 443 . . 444 447 + CviAII CATG 444 446 . . 452 455 + CviJI RGCY 453 453 . . 454 464 + BsiYI* CCNNNNNNNGG 460 457 . . 470 473 + TaqI TCGA 470 472 . . 472 476 + MboII GAAGA 484 483 . . 477 481 + Tsp4CI* ACNGT 479 478 . . 481 484 + CviRI* TGCA 482 482 . . 488 491 + CviRI* TGCA 489 489 . . 500 505 + Hpy178III* TCNNGA 501 503 . . 514 519 + Hpy166II GTNNAC 516 516 . . 517 522 + ZraI GACGTC 519 519 . . 517 522 + AcyI GRCGYC 518 520 . . 517 522 + AatII GACGTC 521 517 . . 518 521 + TaiI ACGT 521 517 . . 518 521 + SetI ASST 521 517 . . 518 521 + MaeII ACGT 518 520 . . 520 524 - BslFI GGGAC 505 509 . . 524 534 + BsiYI* CCNNNNNNNGG 530 527 . . 524 534 + PflMI CCANNNNNTGG 530 527 . . 528 531 + SetI ASST 531 527 . . 533 537 + BslFI GGGAC 547 551 . . 536 539 + TaiI ACGT 539 535 . . 536 539 + SetI ASST 539 535 . . 536 539 + MaeII ACGT 536 538 . . 538 543 + Hpy166II GTNNAC 540 540 . . 538 543 + HindII GTYRAC 540 540 . . 541 550 + PshAI GACNNNNGTC 545 545 . . 546 549 + SetI ASST 549 545 . . 547 551 + BmgT120I GGNCC 548 549 . . 547 551 + AvaII GGWCC 547 550 . . 547 551 + AsuI* GGNCC 547 550 . . 549 553 + Hpy188I TCNGA 551 550 . . 552 555 + DpnI GATC 553 553 . . 552 555 + BstKTI GATC 554 552 . . 552 555 + MboI GATC 551 555 . . 557 561 + DdeI CTNAG 557 560 . . 557 561 + BspCNI CTCAG 570 568 . . 557 561 + BseMII CTCAG 571 569 . . 560 563 + CviJI RGCY 561 561 . . 563 566 + MnlI CCTC 573 572 . . 567 570 + TspEI AATT 566 570 . . 568 573 + VspI ATTAAT 569 571 . . 569 572 + MseI TTAA 569 571 . . 575 580 - EcoP15I CAGCAG 547 549 . . 591 595 + Hpy188I TCNGA 593 592 . . 595 599 + Tsp4CI* ACNGT 597 596 . . 598 602 - BslFI GGGAC 583 587 . . 605 608 + CviJI RGCY 606 606 . . 608 611 + FatI CATG 607 611 . . 608 611 + NlaIII CATG 611 607 . . 608 611 + CviAII CATG 608 610 . . 610 613 + CviRI* TGCA 611 611 . . 611 615 + BlsI GCNGC 613 612 . . 611 615 + BisI GCNGC 612 613 . . 611 615 + BbvI GCAGC 623 627 . . 611 615 + TseI GCWGC 611 614 . . 613 616 + SetI ASST 616 612 . . 613 616 + CviJI RGCY 614 614 . . 613 616 + AluI AGCT 614 614 . . 630 634 + TfiI GAWTC 630 633 . . 630 634 + HinfI GANTC 630 633 . . 646 651 + BsaAI YACGTR 648 648 . . 647 650 + TaiI ACGT 650 646 . . 647 650 + SetI ASST 650 646 . . 647 650 + MaeII ACGT 647 649 . . 656 660 + MboII GAAGA 668 667 . . 659 663 + TfiI GAWTC 659 662 . . 659 663 + HinfI GANTC 659 662 . . 661 665 - TspDTI ATGAA 651 649 . . 667 670 + MnlI CCTC 677 676 . . 668 673 + SmlI CTYRAG 668 672 . . 668 673 + XhoI CTCGAG 668 672 . . 668 673 + BmeT110I CYCGRG 669 671 . . 668 673 + AvaI CYCGRG 668 672 . . 669 672 + TaqI TCGA 669 671 . . 669 674 + Hpy178III* TCNNGA 670 672 . . 685 690 + ApoI RAATTY 685 689 . . 686 689 + TspEI AATT 685 689 . . 691 694 + CviJI RGCY 692 692 . . 698 701 + CviJI RGCY 699 699 . . 707 716 + BsaBI GATNNNNATC 711 711 . . 710 714 + TspDTI ATGAA 725 723 . . 712 716 + TfiI GAWTC 712 715 . . 712 716 + HinfI GANTC 712 715 . . 726 731 + SspI AATATT 728 728 . . 737 741 + Hpy188I TCNGA 739 738 . . #--------------------------------------- # # # AatII: # [GACGTC] # 220 521 # # AccI: # [GTMKAC] # 4 737 # # AciI: # [CCGC] # 212 529 # # AcyI: # [GRCGYC] # 223 518 # # AflIII: # [ACRYGT] # 177 564 # # AluI: # [AGCT] # 7 13 30 53 66 127 # 143 302 # # ApoI: # [RAATTY] # 29 56 298 358 # # AsuI*: # [GGNCC] # 61 135 194 351 # # AvaI: # [CYCGRG] # 73 668 # # AvaII: # [GGWCC] # 61 194 486 # # BbvI: # [GCAGC] # 46 118 577 # # BccI: # [CCATC] # 51 160 530 # # Bce83I*: # [CTTGAG] # 338 403 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 315 392 # # BisI: # [GCNGC] # 35 129 577 # # BlsI: # [GCNGC] # 36 128 577 # # BmeT110I: # [CYCGRG] # 72 669 # # BmgT120I: # [GGNCC] # 62 135 193 351 # # BsaAI: # [YACGTR] # 93 648 # # BsaBI: # [GATNNNNATC] # 30 711 # # BseBI: # [CCWGG] # 200 541 # # BseGI: # [GGATG] # 222 519 # # BseMII: # [CTCAG] # 136 170 435 # # BseYI: # [CCCAGC] # 90 103 548 # # BsiI*: # [CACGAG] # 223 518 # # BsiYI*: # [CCNNNNNNNGG] # 70 70 78 211 312 # # BslFI: # [GGGAC] # 36 42 158 505 # # BsmAI: # [GTCTC] # 130 226 385 # # BspCNI: # [CTCAG] # 137 171 433 # # BspLU11I*: # [ACATGT] # 177 564 # # BsrI: # [ACTGG] # 135 238 368 # # BssKI: # [CCNGG] # 198 543 # # BstKTI: # [GATC] # 187 554 # # Cac8I: # [GCNNGC] # 32 90 167 452 # # CviAII: # [CATG] # 133 164 178 266 # # CviJI: # [RGCY] # 7 7 8 13 15 15 # 21 30 42 45 48 51 # 53 60 66 74 78 108 # # CviRI*: # [TGCA] # 7 11 34 67 122 130 # 370 # # DdeI: # [CTNAG] # 145 184 412 # # DpnI: # [GATC] # 188 553 # # DraII: # [RGGNCCY] # 61 680 # # Eco31I: # [GGTCTC] # 226 515 # # EcoP15I: # [CAGCAG] # 194 547 # # EcoRI: # [GAATTC] # 298 443 # # EcoRII: # [CCWGG] # 198 543 # # EcoT22I: # [ATGCAT] # 335 406 # # FatI: # [CATG] # 134 164 177 266 # # FauI: # [CCCGC] # 205 536 # # FnuDII*: # [CGCG] # 118 623 # # FokI: # [GGATG] # 229 512 # # GlaI: # [GCGC] # 61 112 119 449 # # GsaI: # [CCCAGC] # 90 107 544 # # HaeIII: # [GGCC] # 198 543 # # HhaI: # [GCGC] # 61 112 120 448 # # Hin4I: # [GAYNNNNNVTC] # 32 48 661 # # Hin6I: # [GCGC] # 61 112 118 450 # # HindII: # [GTYRAC] # 201 540 # # HindIII: # [AAGCTT] # 28 216 497 # # HinfI: # [GANTC] # 29 29 53 74 556 # # HpaII: # [CCGG] # 353 388 # # HphI: # [GGTGA] # 310 431 # # Hpy166II: # [GTNNAC] # 5 24 198 201 313 # # Hpy178III*: # [TCNNGA] # 71 146 169 355 # # Hpy188I: # [TCNGA] # 2 42 73 146 478 # # MaeII: # [ACGT] # 7 18 94 111 511 # # MaeIII: # [GTNAC] # 51 202 488 # # MboI: # [GATC] # 190 551 # # MboII: # [GAAGA] # 73 184 484 # # MfeI: # [CAATTG] # 322 419 # # MnlI: # [CCTC] # 64 68 74 95 104 124 # 212 # # MseI: # [TTAA] # 172 569 # # MstI*: # [TGCGCA] # 231 510 # # NlaIII: # [CATG] # 130 164 181 266 # # NlaIV: # [GGNNCC] # 62 679 # # NspBII*: # [CMGCKG] # 37 275 429 # # NspI: # [RCATGY] # 181 560 # # PflMI: # [CCANNNNNTGG] # 211 530 # # PpuMI: # [RGGWCCY] # 61 680 # # PshAI: # [GACNNNNGTC] # 196 545 # # PvuII: # [CAGCTG] # 37 275 429 # # ScrFI: # [CCNGG] # 200 541 # # SetI: # [ASST] # 7 8 10 10 10 13 # 22 34 44 53 66 67 # 86 91 99 121 # # SfaNI: # [GCATC] # 339 402 # # SmlI: # [CTYRAG] # 73 315 353 # # SspI: # [AATATT] # 13 40 309 379 # # TaiI: # [ACGT] # 10 18 91 111 511 # # TaqI: # [TCGA] # 44 72 199 426 # # TfiI: # [GAWTC] # 29 29 53 74 556 # # TseI: # [GCWGC] # 34 130 577 # # TsoI: # [TARCCA] # 242 499 # # Tsp4CI*: # [ACNGT] # 118 144 479 # # TspDTI: # [ATGAA] # 11 16 74 640 # # TspEI: # [AATT] # 5 24 52 56 119 239 # 246 # # TspGWI: # [ACGGA] # 178 563 # # TspRI: # [CASTGNN] # 149 151 441 # # VspI: # [ATTAAT] # 172 569 # # XhoI: # [CTCGAG] # 73 668 # # XmnI: # [GAANNNNTTC] # 331 410 # # ZraI: # [GACGTC] # 222 519 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 741 # Reported_sequences: 1 # Reported_hitcount: 222 #---------------------------------------