Restriction Fragments for YNL150W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YNL150W on chromosome XIV from coordinates 349251 to 349658.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26865.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + CviRI* TGCA 6 6 . . 26 31 - TsoI TARCCA 16 14 . . 42 46 + Hpy188I TCNGA 44 43 . . 50 55 + SfeI* CTRYAG 50 54 . . 77 81 + Tsp45I GTSAC 76 81 . . 77 81 + MaeIII GTNAC 76 81 . . 93 98 + NlaIV GGNNCC 95 95 . . 91 94 - MnlI CCTC 84 83 . . 114 117 + CviJI RGCY 115 115 . . 121 125 - MboII GAAGA 113 112 . . 125 129 - BsrI ACTGG 125 123 . . 134 137 + MnlI CCTC 144 143 . . 145 149 + SfaNI GCATC 154 158 . . 157 162 + NlaIV GGNNCC 159 159 . . 158 162 + BmgT120I GGNCC 159 160 . . 158 162 + AvaII GGWCC 158 161 . . 158 162 + AsuI* GGNCC 158 161 . . 160 165 + MmeI TCCRAC 185 183 . . 161 171 + BsiYI* CCNNNNNNNGG 167 164 . . 161 171 + PflMI CCANNNNNTGG 167 164 . . 166 171 + StyI CCWWGG 166 170 . . 166 171 + SecI* CCNNGG 166 170 . . 174 178 - MboII GAAGA 166 165 . . 180 184 + DdeI CTNAG 180 183 . . 183 186 + CviJI RGCY 184 184 . . 185 188 + MnlI CCTC 195 194 . . 186 191 + Ksp632I* CTCTTC 192 195 . . 187 191 - MboII GAAGA 179 178 . . 223 226 + MseI TTAA 223 225 . . 227 232 + Ksp632I* CTCTTC 233 236 . . 228 232 - MboII GAAGA 220 219 . . 231 235 - MboII GAAGA 223 222 . . 235 238 + MaeI CTAG 235 237 . . 271 281 + MwoI GCNNNNNNNGC 277 274 . . 269 274 - EcoP15I CAGCAG 241 243 . . 276 279 + CviRI* TGCA 277 277 . . 277 281 + BlsI GCNGC 279 278 . . 277 281 + BisI GCNGC 278 279 . . 277 281 + BbvI GCAGC 289 293 . . 277 281 + TseI GCWGC 277 280 . . 280 284 + SfaNI GCATC 289 293 . . 289 292 + CviJI RGCY 290 290 . . 295 298 + TaqI TCGA 295 297 . . 297 300 + DpnI GATC 298 298 . . 297 300 + BstKTI GATC 299 297 . . 297 300 + MboI GATC 296 300 . . 301 304 + SetI ASST 304 300 . . 310 313 + DpnI GATC 311 311 . . 310 313 + BstKTI GATC 312 310 . . 310 313 + MboI GATC 309 313 . . 320 323 + MnlI CCTC 330 329 . . 327 331 + BsmAI GTCTC 332 336 . . 352 356 + BlsI GCNGC 354 353 . . 352 356 + BisI GCNGC 353 354 . . 352 356 + BbvI GCAGC 364 368 . . 352 356 + TseI GCWGC 352 355 . . 352 363 + AlfI GCANNNNNNTGC 341 339 375 373 354 357 + CviJI RGCY 355 355 . . 352 363 - AlfI GCANNNNNNTGC 375 373 341 339 365 369 - MboII GAAGA 357 356 . . 356 369 - AjuI GAANNNNNNNTTGG 381 376 349 344 371 382 + BstXI CCANNNNNNTGG 378 374 . . 373 376 + TspEI AATT 372 376 . . 377 382 + SecI* CCNNGG 377 381 . . 378 382 + BssKI CCNGG 377 382 . . 378 382 + BseBI CCWGG 379 380 . . 378 382 + ScrFI CCNGG 379 380 . . 378 382 + EcoRII CCWGG 377 382 . . 381 385 + FokI GGATG 394 398 . . 381 385 + BseGI GGATG 387 385 . . 382 386 - SfaNI GCATC 372 376 . . 394 399 + SmlI CTYRAG 394 398 . . 394 399 + AflII CTTAAG 394 398 . . 395 398 + MseI TTAA 395 397 . . #--------------------------------------- # # # AflII: # [CTTAAG] # 14 394 # # AjuI: # [GAANNNNNNNTTGG] # 27 32 349 # # AlfI: # [GCANNNNNNTGC] # 0 0 33 34 341 # # AsuI*: # [GGNCC] # 158 250 # # AvaII: # [GGWCC] # 158 250 # # BbvI: # [GCAGC] # 44 75 289 # # BisI: # [GCNGC] # 55 75 278 # # BlsI: # [GCNGC] # 54 75 279 # # BmgT120I: # [GGNCC] # 159 249 # # BseBI: # [CCWGG] # 29 379 # # BseGI: # [GGATG] # 21 387 # # BsiYI*: # [CCNNNNNNNGG] # 167 241 # # BsmAI: # [GTCTC] # 76 332 # # BsrI: # [ACTGG] # 125 283 # # BssKI: # [CCNGG] # 31 377 # # BstKTI: # [GATC] # 13 96 299 # # BstXI: # [CCANNNNNNTGG] # 30 378 # # CviJI: # [RGCY] # 53 65 69 106 115 # # CviRI*: # [TGCA] # 6 131 271 # # DdeI: # [CTNAG] # 180 228 # # DpnI: # [GATC] # 13 97 298 # # EcoP15I: # [CAGCAG] # 167 241 # # EcoRII: # [CCWGG] # 31 377 # # FokI: # [GGATG] # 14 394 # # Hpy188I: # [TCNGA] # 44 364 # # Ksp632I*: # [CTCTTC] # 41 175 192 # # MaeI: # [CTAG] # 173 235 # # MaeIII: # [GTNAC] # 76 332 # # MboI: # [GATC] # 13 99 296 # # MboII: # [GAAGA] # 3 13 41 51 53 113 # 134 # # MmeI: # [TCCRAC] # 185 223 # # MnlI: # [CCTC] # 51 60 78 84 135 # # MseI: # [TTAA] # 13 172 223 # # MwoI: # [GCNNNNNNNGC] # 131 277 # # NlaIV: # [GGNNCC] # 64 95 249 # # PflMI: # [CCANNNNNTGG] # 167 241 # # ScrFI: # [CCNGG] # 29 379 # # SecI*: # [CCNNGG] # 31 166 211 # # SetI: # [ASST] # 104 304 # # SfaNI: # [GCATC] # 36 83 135 154 # # SfeI*: # [CTRYAG] # 50 358 # # SmlI: # [CTYRAG] # 14 394 # # StyI: # [CCWWGG] # 166 242 # # TaqI: # [TCGA] # 113 295 # # TseI: # [GCWGC] # 56 75 277 # # TsoI: # [TARCCA] # 16 392 # # Tsp45I: # [GTSAC] # 76 332 # # TspEI: # [AATT] # 36 372 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 408 # Reported_sequences: 1 # Reported_hitcount: 74 #---------------------------------------