Restriction Fragments for PHO23/YNL097C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PHO23/YNL097C on chromosome XIV from coordinates 442358 to 441366.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30953.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + Hpy166II GTNNAC 7 7 . . 15 24 + XmnI GAANNNNTTC 19 19 . . 17 20 + SetI ASST 20 16 . . 24 29 + SecI* CCNNGG 24 28 . . 25 29 + BssKI CCNGG 24 29 . . 25 29 + BseBI CCWGG 26 27 . . 25 29 + ScrFI CCNGG 26 27 . . 25 29 + EcoRII CCWGG 24 29 . . 31 34 + MseI TTAA 31 33 . . 47 50 + TaiI ACGT 50 46 . . 47 50 + SetI ASST 50 46 . . 47 50 + MaeII ACGT 47 49 . . 46 51 - BtrI CACGTC 48 48 . . 52 57 + Eco57MI CTGRAG 73 71 . . 52 57 + GsuI CTGGAG 73 71 . . 55 58 - MnlI CCTC 48 47 . . 58 63 + EcoRI GAATTC 58 62 . . 58 63 + ApoI RAATTY 58 62 . . 59 62 + TspEI AATT 58 62 . . 65 71 + TspRI CASTGNN 71 62 . . 66 70 + BsrI ACTGG 71 69 . . 68 73 + BalI TGGCCA 70 70 . . 68 73 + CfrI YGGCCR 68 72 . . 69 72 + CviJI RGCY 70 70 . . 69 72 + HaeIII GGCC 70 70 . . 73 76 + SetI ASST 76 72 . . 74 77 + MnlI CCTC 84 83 . . 76 81 + Hpy178III* TCNNGA 77 79 . . 75 80 - Eco57MI CTGRAG 60 58 . . 75 80 - GsuI CTGGAG 60 58 . . 85 88 + MseI TTAA 85 87 . . 99 110 + BcgI CGANNNNNNTGC 88 86 122 120 108 111 + CviRI* TGCA 109 109 . . 106 110 - SfaNI GCATC 96 100 . . 119 122 + CviRI* TGCA 120 120 . . 120 130 + MwoI GCNNNNNNNGC 126 123 . . 120 130 + BstAPI GCANNNNNTGC 126 123 . . 120 125 - BsmI GAATGC 120 118 . . 132 137 + ApoI RAATTY 132 136 . . 133 136 + TspEI AATT 132 136 . . 144 147 - MnlI CCTC 137 136 . . 158 163 + Hpy178III* TCNNGA 159 161 . . 162 166 + MboII GAAGA 174 173 . . 163 173 + FalI AAGNNNNNCTT 154 149 186 181 165 174 + XmnI GAANNNNTTC 169 169 . . 163 173 - FalI AAGNNNNNCTT 186 181 154 149 181 184 + DpnI GATC 182 182 . . 181 184 + BstKTI GATC 183 181 . . 181 184 + MboI GATC 180 184 . . 183 187 - HphI GGTGA 175 174 . . 227 231 + TspDTI ATGAA 242 240 . . 229 233 + MboII GAAGA 241 240 . . 237 241 - SfaNI GCATC 227 231 . . 241 245 + BccI CCATC 249 250 . . 247 252 + Eco57MI CTGRAG 268 266 . . 242 247 - BtgZI GCGATG 227 231 . . 247 252 + GsuI CTGGAG 268 266 . . 250 255 + BseRI GAGGAG 265 263 . . 250 253 - MnlI CCTC 243 242 . . 259 264 + EcoT22I ATGCAT 263 259 . . 260 263 + CviRI* TGCA 261 261 . . 261 266 + NspI RCATGY 265 261 . . 262 265 + FatI CATG 261 265 . . 262 265 + NlaIII CATG 265 261 . . 262 265 + CviAII CATG 262 264 . . 265 269 + BsmAI GTCTC 270 274 . . 272 283 + BstXI CCANNNNNNTGG 279 275 . . 269 273 - FokI GGATG 255 259 . . 269 273 - BseGI GGATG 268 266 . . 288 293 + XbaI TCTAGA 288 292 . . 288 293 + Hpy178III* TCNNGA 289 291 . . 289 292 + MaeI CTAG 289 291 . . 300 305 + ZraI GACGTC 302 302 . . 300 305 + AcyI GRCGYC 301 303 . . 300 305 + AatII GACGTC 304 300 . . 301 304 + TaiI ACGT 304 300 . . 301 304 + SetI ASST 304 300 . . 301 304 + MaeII ACGT 301 303 . . 305 309 + BssKI CCNGG 304 309 . . 305 309 + ScrFI CCNGG 306 307 . . 305 309 + CauII* CCSGG 306 307 . . 306 309 + HpaII CCGG 306 308 . . 303 307 - BslFI GGGAC 288 292 . . 314 317 + TspEI AATT 313 317 . . 323 327 + TspDTI ATGAA 338 336 . . 335 340 + Hpy178III* TCNNGA 336 338 . . 342 353 + TstI CACNNNNNNTCC 333 328 365 360 347 352 + ApoI RAATTY 347 351 . . 348 351 + TspEI AATT 347 351 . . 353 356 + MaeI CTAG 353 355 . . 357 360 + SetI ASST 360 356 . . 356 359 - MnlI CCTC 349 348 . . 361 364 + MseI TTAA 361 363 . . 366 370 + BsrI ACTGG 371 369 . . 366 371 + BfiI ACTGGG 376 375 . . 368 373 - TaqII CACCCA 358 356 . . 373 378 + Hpy166II GTNNAC 375 375 . . 381 385 + BccI CCATC 389 390 . . 382 386 - FokI GGATG 368 372 . . 382 386 - BseGI GGATG 381 379 . . 388 393 + BsrDI GCAATG 395 393 . . 392 395 + CviRI* TGCA 393 393 . . 395 398 + SetI ASST 398 394 . . 396 399 + MnlI CCTC 406 405 . . 399 403 + BccI CCATC 407 408 . . 402 407 + Hpy178III* TCNNGA 403 405 . . 402 407 + BspHI TCATGA 402 406 . . 403 406 + FatI CATG 402 406 . . 403 406 + NlaIII CATG 406 402 . . 403 406 + CviAII CATG 403 405 . . 404 408 + TspDTI ATGAA 419 417 . . 424 434 - Hin4I GAYNNNNNVTC 447 442 415 410 446 450 + Hpy99I CGWCG 450 445 . . 450 455 + Cac8I GCNNGC 452 452 . . 451 459 + AlwNI CAGNNNCTG 456 453 . . 455 461 + TspRI CASTGNN 461 452 . . 457 462 + Eco57MI CTGRAG 478 476 . . 457 462 + Eco57I CTGAAG 478 476 . . 459 463 + MboII GAAGA 471 470 . . 459 464 - Ksp632I* CTCTTC 454 457 . . 459 465 - SapI GCTCTTC 454 457 . . 466 470 + TfiI GAWTC 466 469 . . 466 470 + HinfI GANTC 466 469 . . 469 474 + Hpy178III* TCNNGA 470 472 . . 473 477 + MboII GAAGA 485 484 . . 473 478 - Ksp632I* CTCTTC 468 471 . . 480 483 + CviJI RGCY 481 481 . . 481 491 + MwoI GCNNNNNNNGC 487 484 . . 482 487 + DsaI* CCRYGG 482 486 . . 482 487 + StyI CCWWGG 482 486 . . 482 487 + NcoI CCATGG 482 486 . . 482 487 + SecI* CCNNGG 482 486 . . 483 486 + FatI CATG 482 486 . . 483 486 + NlaIII CATG 486 482 . . 483 486 + CviAII CATG 483 485 . . 486 489 + CviJI RGCY 487 487 . . 487 491 + BlsI GCNGC 489 488 . . 487 491 + BisI GCNGC 488 489 . . 487 491 + TseI GCWGC 487 490 . . 487 491 - BbvI GCAGC 474 478 . . 491 501 + BsiYI* CCNNNNNNNGG 497 494 . . 498 501 - MnlI CCTC 491 490 . . 518 521 + AciI CCGC 518 520 . . 520 525 + Cac8I GCNNGC 522 522 . . 517 522 - EciI GGCGGA 507 505 . . 541 545 + HinfI GANTC 541 544 . . 541 545 - PleI GAGTC 535 536 . . 541 545 - MlyI GAGTC 535 535 . . 552 556 + Hpy99I CGWCG 556 551 . . 553 557 + HgaI GACGC 562 567 . . 563 567 + TspGWI ACGGA 578 576 . . 566 569 - MnlI CCTC 559 558 . . 569 573 + BlsI GCNGC 571 570 . . 569 573 + BisI GCNGC 570 571 . . 569 573 + BbvI GCAGC 581 585 . . 569 573 + TseI GCWGC 569 572 . . 570 575 + EcoP15I CAGCAG 600 602 . . 572 577 + Cac8I GCNNGC 574 574 . . 577 582 + BsiI* CACGAG 577 581 . . 583 586 + CviJI RGCY 584 584 . . 585 599 + XcmI CCANNNNNNNNNTGG 592 591 . . 594 600 + TspRI CASTGNN 600 591 . . 595 599 + BsrI ACTGG 600 598 . . 599 603 + MaeIII GTNAC 598 603 . . 612 617 + SmlI CTYRAG 612 616 . . 613 618 + Hpy178III* TCNNGA 614 616 . . 612 617 - Bce83I* CTTGAG 597 595 . . 624 627 + CviJI RGCY 625 625 . . 625 635 + MwoI GCNNNNNNNGC 631 628 . . 631 635 + BlsI GCNGC 633 632 . . 631 635 + BisI GCNGC 632 633 . . 631 635 + TseI GCWGC 631 634 . . 631 635 - BbvI GCAGC 618 622 . . 652 655 + DpnI GATC 653 653 . . 652 655 + BstKTI GATC 654 652 . . 652 655 + MboI GATC 651 655 . . 654 659 + Hpy178III* TCNNGA 655 657 . . 652 656 - BinI* GGATC 646 647 . . 658 662 - BsmAI GTCTC 652 656 . . 658 663 - Eco31I GGTCTC 652 656 . . 675 680 + BseRI GAGGAG 690 688 . . 675 678 - MnlI CCTC 668 667 . . 685 695 + MwoI GCNNNNNNNGC 691 688 . . 693 696 + CviJI RGCY 694 694 . . 694 698 - BceAI ACGGC 679 681 . . 700 704 - HphI GGTGA 692 691 . . 707 717 + MwoI GCNNNNNNNGC 713 710 . . 718 722 + BceAI ACGGC 734 736 . . 727 731 - BceAI ACGGC 712 714 . . 747 750 + SetI ASST 750 746 . . 749 752 + Csp6I GTAC 749 751 . . 749 752 + RsaI GTAC 750 750 . . 755 760 + SfeI* CTRYAG 755 759 . . 760 764 - HgaI GACGC 749 754 . . 764 768 + BssKI CCNGG 763 768 . . 764 768 + BseBI CCWGG 765 766 . . 764 769 + SecI* CCNNGG 764 768 . . 764 768 + ScrFI CCNGG 765 766 . . 764 768 + EcoRII CCWGG 763 768 . . 776 780 + BlsI GCNGC 778 777 . . 776 780 + BisI GCNGC 777 778 . . 776 780 + BbvI GCAGC 788 792 . . 776 780 + TseI GCWGC 776 779 . . 780 784 + Hpy99I CGWCG 784 779 . . 782 786 + Hpy188I TCNGA 784 783 . . 779 783 - HgaI GACGC 768 773 . . 781 786 - MmeI TCCRAC 762 760 . . 791 801 + MwoI GCNNNNNNNGC 797 794 . . 798 803 + EcoP15I CAGCAG 828 830 . . 804 809 + EcoRV GATATC 806 806 . . 808 813 + Hpy178III* TCNNGA 809 811 . . 830 834 + BceAI ACGGC 846 848 . . 838 841 + AciI CCGC 838 840 . . 838 843 + BsrBI CCGCTC 840 840 . . 851 855 + Tsp4CI* ACNGT 853 852 . . 854 858 + MaeIII GTNAC 853 858 . . 855 860 + TsoI TARCCA 871 869 . . 893 896 + Hin6I GCGC 893 895 . . 893 896 + GlaI GCGC 894 894 . . 889 893 - BccI CCATC 883 884 . . 893 896 + HhaI GCGC 895 893 . . 899 903 + Tsp4CI* ACNGT 901 900 . . 905 908 + SetI ASST 908 904 . . 905 908 + CviJI RGCY 906 906 . . 905 908 + AluI AGCT 906 906 . . 907 910 + MaeI CTAG 907 909 . . 914 919 + NlaIV GGNNCC 916 916 . . 925 935 + BsiYI* CCNNNNNNNGG 931 928 . . 925 935 + PflMI CCANNNNNTGG 931 928 . . 926 929 + FatI CATG 925 929 . . 926 929 + NlaIII CATG 929 925 . . 926 929 + CviAII CATG 926 928 . . 935 939 + HinfI GANTC 935 938 . . 938 941 + TaqI TCGA 938 940 . . 935 939 - PleI GAGTC 929 930 . . 935 939 - MlyI GAGTC 929 929 . . 948 951 + SetI ASST 951 947 . . 949 955 + SauI* CCTNAGG 950 953 . . 950 954 + DdeI CTNAG 950 953 . . 968 978 + MwoI GCNNNNNNNGC 974 971 . . 969 973 + Hpy99I CGWCG 973 968 . . 976 979 + CviRI* TGCA 977 977 . . 987 991 + Tsp4CI* ACNGT 989 988 . . #--------------------------------------- # # # AatII: # [GACGTC] # 304 689 # # AciI: # [CCGC] # 155 320 518 # # AcyI: # [GRCGYC] # 301 692 # # AluI: # [AGCT] # 87 906 # # AlwNI: # [CAGNNNCTG] # 456 537 # # ApoI: # [RAATTY] # 58 74 215 646 # # BalI: # [TGGCCA] # 70 923 # # BbvI: # [GCAGC] # 37 107 170 205 474 # # BccI: # [CCATC] # 18 110 140 249 476 # # Bce83I*: # [CTTGAG] # 396 597 # # BceAI: # [ACGGC] # 22 33 112 147 679 # # BcgI: # [CGANNNNNNTGC] # 34 88 871 # # BfiI: # [ACTGGG] # 376 617 # # BinI*: # [GGATC] # 347 646 # # BisI: # [GCNGC] # 62 82 145 216 488 # # BlsI: # [GCNGC] # 62 82 145 215 489 # # BseBI: # [CCWGG] # 26 228 739 # # BseGI: # [GGATG] # 113 268 612 # # BseRI: # [GAGGAG] # 265 303 425 # # BsiI*: # [CACGAG] # 416 577 # # BsiYI*: # [CCNNNNNNNGG] # 62 434 497 # # BslFI: # [GGGAC] # 288 705 # # BsmAI: # [GTCTC] # 270 341 382 # # BsmI: # [GAATGC] # 120 873 # # BspHI: # [TCATGA] # 402 591 # # BsrBI: # [CCGCTC] # 153 840 # # BsrDI: # [GCAATG] # 395 598 # # BsrI: # [ACTGG] # 71 229 300 393 # # BssKI: # [CCNGG] # 24 230 280 459 # # BstAPI: # [GCANNNNNTGC] # 126 867 # # BstKTI: # [GATC] # 183 339 471 # # BstXI: # [CCANNNNNNTGG] # 279 714 # # BtgZI: # [GCGATG] # 227 766 # # BtrI: # [CACGTC] # 48 945 # # Cac8I: # [GCNNGC] # 52 70 419 452 # # CauII*: # [CCSGG] # 306 687 # # CfrI: # [YGGCCR] # 68 925 # # Csp6I: # [GTAC] # 244 749 # # CviAII: # [CATG] # 67 80 141 262 443 # # CviJI: # [RGCY] # 6 41 69 70 87 97 # 212 411 # # CviRI*: # [TGCA] # 11 16 109 132 141 584 # # DdeI: # [CTNAG] # 43 950 # # DpnI: # [GATC] # 182 340 471 # # DsaI*: # [CCRYGG] # 482 511 # # EciI: # [GGCGGA] # 486 507 # # Eco31I: # [GGTCTC] # 341 652 # # Eco57I: # [CTGAAG] # 478 515 # # Eco57MI: # [CTGRAG] # 13 60 195 210 515 # # EcoP15I: # [CAGCAG] # 165 228 600 # # EcoRI: # [GAATTC] # 58 935 # # EcoRII: # [CCWGG] # 24 230 739 # # EcoRV: # [GATATC] # 187 806 # # EcoT22I: # [ATGCAT] # 263 730 # # FalI: # [AAGNNNNNCTT] # 0 0 32 154 807 # # FatI: # [CATG] # 68 80 141 261 443 # # FokI: # [GGATG] # 113 255 625 # # GlaI: # [GCGC] # 99 894 # # GsuI: # [CTGGAG] # 13 60 195 725 # # HaeIII: # [GGCC] # 70 923 # # HgaI: # [GACGC] # 19 187 225 562 # # HhaI: # [GCGC] # 98 895 # # Hin4I: # [GAYNNNNNVTC] # 32 415 546 # # Hin6I: # [GCGC] # 100 893 # # HinfI: # [GANTC] # 58 75 394 466 # # HpaII: # [CCGG] # 306 687 # # HphI: # [GGTGA] # 175 301 517 # # Hpy166II: # [GTNNAC] # 7 368 618 # # Hpy178III*: # [TCNNGA] # 41 47 67 67 77 82 # 130 144 154 184 # # Hpy188I: # [TCNGA] # 209 784 # # Hpy99I: # [CGWCG] # 20 106 189 228 450 # # Ksp632I*: # [CTCTTC] # 14 454 525 # # MaeI: # [CTAG] # 64 86 289 554 # # MaeII: # [ACGT] # 47 254 692 # # MaeIII: # [GTNAC] # 140 255 598 # # MboI: # [GATC] # 180 342 471 # # MboII: # [GAAGA] # 14 67 174 230 508 # # MlyI: # [GAGTC] # 64 394 535 # # MmeI: # [TCCRAC] # 231 762 # # MnlI: # [CCTC] # 36 48 53 57 68 85 # 106 106 109 325 # # MseI: # [TTAA] # 31 54 276 632 # # MwoI: # [GCNNNNNNNGC] # 19 22 60 84 126 144 # 177 361 # # NcoI: # [CCATGG] # 482 511 # # NlaIII: # [CATG] # 64 80 141 265 443 # # NlaIV: # [GGNNCC] # 77 916 # # NspI: # [RCATGY] # 265 728 # # PflMI: # [CCANNNNNTGG] # 62 931 # # PleI: # [GAGTC] # 64 394 535 # # RsaI: # [GTAC] # 243 750 # # SapI: # [GCTCTTC] # 454 539 # # SauI*: # [CCTNAGG] # 43 950 # # ScrFI: # [CCNGG] # 26 228 280 459 # # SecI*: # [CCNNGG] # 24 229 282 458 # # SetI: # [ASST] # 20 26 30 38 42 43 # 56 158 228 352 # # SfaNI: # [GCATC] # 96 131 766 # # SfeI*: # [CTRYAG] # 238 755 # # SmlI: # [CTYRAG] # 381 612 # # StyI: # [CCWWGG] # 482 511 # # TaiI: # [ACGT] # 50 254 689 # # TaqI: # [TCGA] # 55 938 # # TaqII: # [CACCCA] # 358 635 # # TfiI: # [GAWTC] # 466 527 # # TseI: # [GCWGC] # 62 82 145 217 487 # # TsoI: # [TARCCA] # 122 871 # # Tsp4CI*: # [ACNGT] # 4 48 88 853 # # TspDTI: # [ATGAA] # 81 96 242 574 # # TspEI: # [AATT] # 34 58 74 181 646 # # TspGWI: # [ACGGA] # 415 578 # # TspRI: # [CASTGNN] # 71 139 390 393 # # TstI: # [CACNNNNNNTCC] # 32 333 628 # # XbaI: # [TCTAGA] # 288 705 # # XcmI: # [CCANNNNNNNNNTGG] # 401 592 # # XmnI: # [GAANNNNTTC] # 19 150 824 # # ZraI: # [GACGTC] # 302 691 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 993 # Reported_sequences: 1 # Reported_hitcount: 243 #---------------------------------------