Restriction Fragments for TOP2/YNL088W



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The currently selected gene/sequence is :

TOP2/YNL088W on chromosome XIV from coordinates 457704 to 461990.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.20569.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 3 8 + HindII GTYRAC 5 5 . . 12 17 + Cfr10I RCCGGY 12 16 . . 12 17 + BetI* WCCGGW 12 16 . . 12 17 + AgeI ACCGGT 12 16 . . 13 16 + HpaII CCGG 13 15 . . 19 24 + HaeII RGCGCY 23 19 . . 20 23 + Hin6I GCGC 20 22 . . 20 23 + GlaI GCGC 21 21 . . 20 23 + HhaI GCGC 22 20 . . 23 26 + MnlI CCTC 33 32 . . 25 29 + Hpy188I TCNGA 27 26 . . 27 38 + BdaI TGANNNNNNTCA 16 14 50 48 28 37 + BsaBI GATNNNNATC 32 32 . . 36 40 + Hpy188I TCNGA 38 37 . . 27 38 - BdaI TGANNNNNNTCA 50 48 16 14 41 46 + ApoI RAATTY 41 45 . . 42 45 + TspEI AATT 41 45 . . 51 55 + BsrI ACTGG 56 54 . . 64 67 + MseI TTAA 64 66 . . 101 106 + SmlI CTYRAG 101 105 . . 102 107 + Hpy178III* TCNNGA 103 105 . . 101 106 - Bce83I* CTTGAG 86 84 . . 108 112 + BlsI GCNGC 110 109 . . 108 112 + BisI GCNGC 109 110 . . 108 112 + BbvI GCAGC 120 124 . . 108 112 + TseI GCWGC 108 111 . . 109 114 + PvuII CAGCTG 111 111 . . 109 114 + NspBII* CMGCKG 111 111 . . 110 113 + SetI ASST 113 109 . . 110 113 + CviJI RGCY 111 111 . . 110 113 + AluI AGCT 111 111 . . 111 115 + BlsI GCNGC 113 112 . . 111 115 + BisI GCNGC 112 113 . . 111 115 + TseI GCWGC 111 114 . . 113 116 + CviRI* TGCA 114 114 . . 114 119 + BsrDI GCAATG 121 119 . . 111 115 - BbvI GCAGC 98 102 . . 128 132 + TspDTI ATGAA 143 141 . . 130 134 + MboII GAAGA 142 141 . . 135 140 + TaqII GACCGA 151 149 . . 130 135 - Ksp632I* CTCTTC 125 128 . . 133 137 - BsmAI GTCTC 127 131 . . 133 138 - Eco31I GGTCTC 127 131 . . 142 145 + CviRI* TGCA 143 143 . . 144 147 + FatI CATG 143 147 . . 144 147 + NlaIII CATG 147 143 . . 144 147 + CviAII CATG 144 146 . . 160 164 + Tsp45I GTSAC 159 164 . . 160 164 + MaeIII GTNAC 159 164 . . 163 173 + BaeI ACNNNNGTAYC 152 147 185 180 164 169 + MfeI CAATTG 164 168 . . 165 168 + TspEI AATT 164 168 . . 169 172 + Csp6I GTAC 169 171 . . 169 172 + RsaI GTAC 170 170 . . 172 176 + BssKI CCNGG 171 176 . . 172 176 + BseBI CCWGG 173 174 . . 172 177 + SecI* CCNNGG 172 176 . . 172 176 + ScrFI CCNGG 173 174 . . 172 176 + EcoRII CCWGG 171 176 . . 194 198 + TspDTI ATGAA 209 207 . . 201 205 + DdeI CTNAG 201 204 . . 211 215 + TauI GCSGC 214 211 . . 211 215 + BlsI GCNGC 213 212 . . 211 215 + BisI GCNGC 212 213 . . 211 214 - AciI CCGC 211 213 . . 228 233 + Hpy178III* TCNNGA 229 231 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 235 239 + BccI CCATC 243 244 . . 232 236 - BinI* GGATC 226 227 . . 237 242 + ClaI ATCGAT 238 240 . . 238 241 + TaqI TCGA 238 240 . . 241 245 + TspDTI ATGAA 256 254 . . 248 252 + TfiI GAWTC 248 251 . . 248 252 + HinfI GANTC 248 251 . . 250 255 + ClaI ATCGAT 251 253 . . 251 254 + TaqI TCGA 251 253 . . 256 261 + Hpy166II GTNNAC 258 258 . . 264 269 + NspI RCATGY 268 264 . . 265 268 + FatI CATG 264 268 . . 265 268 + NlaIII CATG 268 264 . . 265 268 + CviAII CATG 265 267 . . 269 273 + DdeI CTNAG 269 272 . . 269 273 - BspCNI CTCAG 261 259 . . 269 273 - BseMII CTCAG 260 258 . . 271 274 - MnlI CCTC 264 263 . . 268 274 - Bpu10I CCTNAGC 269 272 . . 268 274 - BbvCI CCTCAGC 269 272 . . 281 286 + SfeI* CTRYAG 281 285 . . 298 302 - BccI CCATC 292 293 . . 306 309 + SetI ASST 309 305 . . 321 325 + TfiI GAWTC 321 324 . . 321 325 + HinfI GANTC 321 324 . . 323 327 - TspDTI ATGAA 313 311 . . 337 342 + SspI AATATT 339 339 . . 348 353 + Hpy178III* TCNNGA 349 351 . . 352 365 + AjuI GAANNNNNNNTTGG 344 339 376 371 357 367 + Hin4I GAYNNNNNVTC 348 343 380 375 357 367 - Hin4I GAYNNNNNVTC 380 375 348 343 372 377 + Hpy166II GTNNAC 374 374 . . 372 377 + HindII GTYRAC 374 374 . . 380 384 - FokI GGATG 366 370 . . 380 384 - BseGI GGATG 379 377 . . 385 388 + TspEI AATT 384 388 . . 402 406 + MboII GAAGA 414 413 . . 409 413 + Tsp45I GTSAC 408 413 . . 409 413 + MaeIII GTNAC 408 413 . . 411 417 + TspRI CASTGNN 417 408 . . 412 416 + BsrI ACTGG 417 415 . . 425 429 + Tsp4CI* ACNGT 427 426 . . 436 442 + EspI* GCTNAGC 437 440 . . 437 441 + DdeI CTNAG 437 440 . . 439 444 + HindIII AAGCTT 439 443 . . 440 443 + SetI ASST 443 439 . . 440 443 + CviJI RGCY 441 441 . . 440 443 + AluI AGCT 441 441 . . 459 465 + TspRI CASTGNN 465 456 . . 463 468 + EcoRI GAATTC 463 467 . . 463 468 + ApoI RAATTY 463 467 . . 464 467 + TspEI AATT 463 467 . . 466 470 - TspDTI ATGAA 456 454 . . 479 484 + PstI CTGCAG 483 479 . . 479 484 + SfeI* CTRYAG 479 483 . . 480 483 + CviRI* TGCA 481 481 . . 483 488 + XhoII RGATCY 483 487 . . 483 488 + BglII AGATCT 483 487 . . 484 487 + DpnI GATC 485 485 . . 484 487 + BstKTI GATC 486 484 . . 484 487 + MboI GATC 483 487 . . 495 500 + BalI TGGCCA 497 497 . . 495 500 + CfrI YGGCCR 495 499 . . 496 499 + CviJI RGCY 497 497 . . 496 499 + HaeIII GGCC 497 497 . . 528 531 + FatI CATG 527 531 . . 528 531 + NlaIII CATG 531 527 . . 528 531 + CviAII CATG 528 530 . . 570 575 + NlaIV GGNNCC 572 572 . . 567 571 - Hin4II* CCTTC 561 560 . . 571 575 + BmgT120I GGNCC 572 573 . . 571 575 + AvaII GGWCC 571 574 . . 571 575 + AsuI* GGNCC 571 574 . . 574 578 + BccI CCATC 582 583 . . 587 590 + SetI ASST 590 586 . . 588 592 + HphI GGTGA 600 599 . . 589 593 + Tsp45I GTSAC 588 593 . . 589 593 + MaeIII GTNAC 588 593 . . 596 599 + MseI TTAA 596 598 . . 599 602 + CviJI RGCY 600 600 . . 601 604 + HpaII CCGG 601 603 . . 607 610 + MseI TTAA 607 609 . . 608 613 + TsoI TARCCA 624 622 . . 614 618 + TfiI GAWTC 614 617 . . 614 618 + HinfI GANTC 614 617 . . 617 621 + Hpy188I TCNGA 619 618 . . 622 626 + TspDTI ATGAA 637 635 . . 629 632 + SetI ASST 632 628 . . 629 632 + CviJI RGCY 630 630 . . 629 632 + AluI AGCT 630 630 . . 631 634 + MaeI CTAG 631 633 . . 640 645 + EcoRV GATATC 642 642 . . 645 649 + DdeI CTNAG 645 648 . . 654 658 - SfaNI GCATC 644 648 . . 659 663 + MboII GAAGA 671 670 . . 662 666 + MboII GAAGA 674 673 . . 662 667 - Ksp632I* CTCTTC 657 660 . . 673 678 + EcoRV GATATC 675 675 . . 690 695 + Hpy178III* TCNNGA 691 693 . . 692 696 + Tsp45I GTSAC 691 696 . . 692 696 + MaeIII GTNAC 691 696 . . 694 705 + DrdI GACNNNNNNGTC 700 698 . . 697 702 + VspI ATTAAT 698 700 . . 698 701 + MseI TTAA 698 700 . . 708 712 + Hpy188I TCNGA 710 709 . . 724 727 + MseI TTAA 724 726 . . 735 740 + ApoI RAATTY 735 739 . . 736 739 + TspEI AATT 735 739 . . 745 748 + TspEI AATT 744 748 . . 773 776 + TaqI TCGA 773 775 . . 790 793 + MaeI CTAG 790 792 . . 797 801 + Tsp4CI* ACNGT 799 798 . . 799 803 + HphI GGTGA 811 810 . . 802 805 - MnlI CCTC 795 794 . . 806 810 + Tsp4CI* ACNGT 808 807 . . 808 813 + NlaIV GGNNCC 810 810 . . 808 813 + HgiCI* GGYRCC 808 812 . . 811 815 + TauI GCSGC 814 811 . . 811 815 + BlsI GCNGC 813 812 . . 811 815 + BisI GCNGC 812 813 . . 812 815 + AciI CCGC 812 814 . . 815 819 + DdeI CTNAG 815 818 . . 820 824 + Hpy188I TCNGA 822 821 . . 823 828 + EcoRV GATATC 825 825 . . 827 832 + Hpy178III* TCNNGA 828 830 . . 860 864 - BccI CCATC 854 855 . . 877 880 - AciI CCGC 877 879 . . 883 887 + Hpy188I TCNGA 885 884 . . 886 891 + EcoRV GATATC 888 888 . . 902 907 + ApoI RAATTY 902 906 . . 903 906 + TspEI AATT 902 906 . . 915 920 + EcoRI GAATTC 915 919 . . 915 920 + ApoI RAATTY 915 919 . . 916 919 + TspEI AATT 915 919 . . 924 927 + CviRI* TGCA 925 925 . . 921 926 - BsrDI GCAATG 920 918 . . 932 937 + DsaI* CCRYGG 932 936 . . 932 937 + StyI CCWWGG 932 936 . . 932 937 + NcoI CCATGG 932 936 . . 932 937 + SecI* CCNNGG 932 936 . . 933 936 + FatI CATG 932 936 . . 933 936 + NlaIII CATG 936 932 . . 933 936 + CviAII CATG 933 935 . . 935 940 - TaqII CACCCA 925 923 . . 940 945 + KpnI GGTACC 944 940 . . 940 945 + NlaIV GGNNCC 942 942 . . 940 945 + HgiCI* GGYRCC 940 944 . . 940 945 + Acc65I GGTACC 940 944 . . 941 944 + Csp6I GTAC 941 943 . . 941 944 + RsaI GTAC 942 942 . . 946 949 + FatI CATG 945 949 . . 946 949 + NlaIII CATG 949 945 . . 946 949 + CviAII CATG 946 948 . . 940 950 - BaeI ACNNNNGTAYC 965 960 932 927 952 955 + TspEI AATT 951 955 . . 969 972 + TspEI AATT 968 972 . . 980 985 + ApoI RAATTY 980 984 . . 981 984 + TspEI AATT 980 984 . . 985 989 + Hpy188I TCNGA 987 986 . . 989 994 + ApoI RAATTY 989 993 . . 990 993 + TspEI AATT 989 993 . . 996 1000 + MboII GAAGA 1008 1007 . . 1005 1009 + MboII GAAGA 1017 1016 . . 1029 1033 + Hpy188I TCNGA 1031 1030 . . 1034 1037 + MseI TTAA 1034 1036 . . 1048 1052 - TspDTI ATGAA 1038 1036 . . 1057 1062 + VspI ATTAAT 1058 1060 . . 1054 1058 - TspDTI ATGAA 1044 1042 . . 1058 1061 + MseI TTAA 1058 1060 . . 1060 1063 + TspEI AATT 1059 1063 . . 1068 1078 + Hin4I GAYNNNNNVTC 1059 1054 1091 1086 1074 1078 + TfiI GAWTC 1074 1077 . . 1074 1078 + HinfI GANTC 1074 1077 . . 1068 1078 - Hin4I GAYNNNNNVTC 1091 1086 1059 1054 1080 1083 + CviRI* TGCA 1081 1081 . . 1087 1090 + SetI ASST 1090 1086 . . 1088 1091 + MnlI CCTC 1098 1097 . . 1118 1122 + PleI GAGTC 1126 1127 . . 1118 1122 + MlyI GAGTC 1127 1127 . . 1118 1122 + HinfI GANTC 1118 1121 . . 1126 1136 + Hin4I GAYNNNNNVTC 1117 1112 1149 1144 1132 1138 + SanDI GGGWCCC 1133 1136 . . 1132 1138 + PpuMI RGGWCCY 1133 1136 . . 1132 1138 + DraII RGGNCCY 1133 1136 . . 1132 1137 + NlaIV GGNNCC 1134 1134 . . 1133 1138 + NlaIV GGNNCC 1135 1135 . . 1133 1137 + BmgT120I GGNCC 1134 1135 . . 1133 1137 + AvaII GGWCC 1133 1136 . . 1133 1137 + AsuI* GGNCC 1133 1136 . . 1126 1136 - Hin4I GAYNNNNNVTC 1149 1144 1117 1112 1134 1138 - BslFI GGGAC 1119 1123 . . 1146 1150 + TfiI GAWTC 1146 1149 . . 1146 1150 + HinfI GANTC 1146 1149 . . 1150 1153 + MnlI CCTC 1160 1159 . . 1152 1157 + Hpy178III* TCNNGA 1153 1155 . . 1162 1167 + VspI ATTAAT 1163 1165 . . 1163 1166 + MseI TTAA 1163 1165 . . 1174 1178 + TspDTI ATGAA 1189 1187 . . 1176 1189 + AjuI GAANNNNNNNTTGG 1168 1163 1200 1195 1188 1191 + CviJI RGCY 1189 1189 . . 1187 1192 - TsoI TARCCA 1177 1175 . . 1206 1209 + TspEI AATT 1205 1209 . . 1212 1216 + Hpy99I CGWCG 1216 1211 . . 1213 1217 + HgaI GACGC 1222 1227 . . 1220 1224 + TspDTI ATGAA 1235 1233 . . 1222 1226 + MboII GAAGA 1234 1233 . . 1231 1234 + Hin6I GCGC 1231 1233 . . 1231 1234 + GlaI GCGC 1232 1232 . . 1231 1234 + HhaI GCGC 1233 1231 . . 1243 1247 + Hpy188I TCNGA 1245 1244 . . 1250 1253 + Csp6I GTAC 1250 1252 . . 1246 1250 - BccI CCATC 1240 1241 . . 1250 1253 + RsaI GTAC 1251 1251 . . 1266 1269 + TspEI AATT 1265 1269 . . 1273 1276 + TspEI AATT 1272 1276 . . 1278 1288 + BsiYI* CCNNNNNNNGG 1284 1281 . . 1284 1288 + BsrI ACTGG 1289 1287 . . 1288 1292 + MboII GAAGA 1300 1299 . . 1294 1304 + MwoI GCNNNNNNNGC 1300 1297 . . 1291 1295 - SfaNI GCATC 1281 1285 . . 1302 1305 + CviJI RGCY 1303 1303 . . 1303 1308 + Cfr10I RCCGGY 1303 1307 . . 1304 1307 + HpaII CCGG 1304 1306 . . 1307 1310 + Csp6I GTAC 1307 1309 . . 1307 1310 + RsaI GTAC 1308 1308 . . 1318 1321 + CviJI RGCY 1319 1319 . . 1315 1319 - Hin4II* CCTTC 1309 1308 . . 1327 1332 + TatI WGTACW 1327 1331 . . 1328 1331 + Csp6I GTAC 1328 1330 . . 1328 1331 + RsaI GTAC 1329 1329 . . 1338 1342 + Hpy188I TCNGA 1340 1339 . . 1345 1349 - Hin4II* CCTTC 1339 1338 . . 1351 1355 + TfiI GAWTC 1351 1354 . . 1351 1355 + HinfI GANTC 1351 1354 . . 1355 1358 + AciI CCGC 1355 1357 . . 1354 1359 - EciI GGCGGA 1344 1342 . . 1368 1371 + SetI ASST 1371 1367 . . 1368 1371 + CviJI RGCY 1369 1369 . . 1368 1371 + AluI AGCT 1369 1369 . . 1374 1377 + CviRI* TGCA 1375 1375 . . 1375 1385 + MwoI GCNNNNNNNGC 1381 1378 . . 1377 1380 + SetI ASST 1380 1376 . . 1375 1380 - BspMI ACCTGC 1366 1370 . . 1383 1386 + SetI ASST 1386 1382 . . 1383 1386 + CviJI RGCY 1384 1384 . . 1383 1386 + AluI AGCT 1384 1384 . . 1417 1427 + BsiYI* CCNNNNNNNGG 1423 1420 . . 1417 1427 + PflMI CCANNNNNTGG 1423 1420 . . 1418 1426 + DraIII CACNNNGTG 1423 1420 . . 1449 1452 + SetI ASST 1452 1448 . . 1449 1452 + CviJI RGCY 1450 1450 . . 1449 1452 + AluI AGCT 1450 1450 . . 1451 1454 + MaeI CTAG 1451 1453 . . 1458 1463 + BclI TGATCA 1458 1462 . . 1459 1462 + DpnI GATC 1460 1460 . . 1459 1462 + BstKTI GATC 1461 1459 . . 1459 1462 + MboI GATC 1458 1462 . . 1461 1465 + Hpy188I TCNGA 1463 1462 . . 1476 1479 + FnuDII* CGCG 1477 1477 . . 1477 1480 - AciI CCGC 1477 1479 . . 1481 1486 + ApoI RAATTY 1481 1485 . . 1482 1485 + TspEI AATT 1481 1485 . . 1488 1491 + CviJI RGCY 1489 1489 . . 1493 1496 + MseI TTAA 1493 1495 . . 1500 1503 + TspEI AATT 1499 1503 . . 1509 1513 + MaeIII GTNAC 1508 1513 . . 1516 1521 - BtgZI GCGATG 1501 1505 . . 1529 1533 + TspDTI ATGAA 1544 1542 . . 1531 1535 + MboII GAAGA 1543 1542 . . 1534 1544 + Hin4I GAYNNNNNVTC 1525 1520 1557 1552 1534 1544 - Hin4I GAYNNNNNVTC 1557 1552 1525 1520 1546 1549 + MseI TTAA 1546 1548 . . 1557 1561 + SfaNI GCATC 1566 1570 . . 1565 1570 + BclI TGATCA 1565 1569 . . 1566 1569 + DpnI GATC 1567 1567 . . 1566 1569 + BstKTI GATC 1568 1566 . . 1566 1569 + MboI GATC 1565 1569 . . 1568 1573 + Hpy178III* TCNNGA 1569 1571 . . 1568 1573 + BspHI TCATGA 1568 1572 . . 1569 1572 + FatI CATG 1568 1572 . . 1569 1572 + NlaIII CATG 1572 1568 . . 1569 1572 + CviAII CATG 1569 1571 . . 1572 1577 + TaqII GACCGA 1588 1586 . . 1576 1579 + DpnI GATC 1577 1577 . . 1576 1579 + BstKTI GATC 1578 1576 . . 1576 1585 + BsaBI GATNNNNATC 1580 1580 . . 1576 1579 + MboI GATC 1575 1579 . . 1578 1583 + Hpy178III* TCNNGA 1579 1581 . . 1582 1585 + DpnI GATC 1583 1583 . . 1582 1585 + BstKTI GATC 1584 1582 . . 1582 1585 + MboI GATC 1581 1585 . . 1584 1589 + Hpy178III* TCNNGA 1585 1587 . . 1584 1589 + BspHI TCATGA 1584 1588 . . 1585 1588 + FatI CATG 1584 1588 . . 1585 1588 + NlaIII CATG 1588 1584 . . 1585 1588 + CviAII CATG 1585 1587 . . 1588 1592 - BccI CCATC 1582 1583 . . 1601 1604 + MseI TTAA 1601 1603 . . 1605 1608 + SetI ASST 1608 1604 . . 1609 1612 + MseI TTAA 1609 1611 . . 1611 1614 + TspEI AATT 1610 1614 . . 1613 1618 + PsiI TTATAA 1615 1615 . . 1630 1633 + SetI ASST 1633 1629 . . 1630 1633 + CviJI RGCY 1631 1631 . . 1630 1633 + AluI AGCT 1631 1631 . . 1639 1643 + BssKI CCNGG 1638 1643 . . 1639 1643 + BseBI CCWGG 1640 1641 . . 1639 1643 + ScrFI CCNGG 1640 1641 . . 1639 1643 + EcoRII CCWGG 1638 1643 . . 1651 1656 + EcoRV GATATC 1653 1653 . . 1656 1661 + StyI CCWWGG 1656 1660 . . 1656 1661 + SecI* CCNNGG 1656 1660 . . 1659 1662 + SetI ASST 1662 1658 . . 1672 1677 + EcoRI GAATTC 1672 1676 . . 1672 1677 + ApoI RAATTY 1672 1676 . . 1673 1676 + TspEI AATT 1672 1676 . . 1675 1679 - TspDTI ATGAA 1665 1663 . . 1683 1687 + Hpy188I TCNGA 1685 1684 . . 1686 1689 + DpnI GATC 1687 1687 . . 1686 1689 + BstKTI GATC 1688 1686 . . 1686 1689 + MboI GATC 1685 1689 . . 1700 1704 + BccI CCATC 1708 1709 . . 1728 1731 + CviRI* TGCA 1729 1729 . . 1729 1734 - BsmI GAATGC 1729 1727 . . 1744 1747 + HpaII CCGG 1744 1746 . . 1751 1755 + TspDTI ATGAA 1766 1764 . . 1765 1768 - MnlI CCTC 1758 1757 . . 1768 1772 + MboII GAAGA 1780 1779 . . 1771 1775 + TfiI GAWTC 1771 1774 . . 1771 1775 + HinfI GANTC 1771 1774 . . 1765 1775 - Hin4I GAYNNNNNVTC 1788 1783 1756 1751 1780 1785 + ApoI RAATTY 1780 1784 . . 1781 1784 + TspEI AATT 1780 1784 . . 1803 1808 + PsiI TTATAA 1805 1805 . . 1816 1820 + BslFI GGGAC 1830 1834 . . 1825 1828 + MaeI CTAG 1825 1827 . . 1829 1840 + TstI CACNNNNNNTCC 1820 1815 1852 1847 1838 1842 + Hpy188I TCNGA 1840 1839 . . 1844 1849 + SspI AATATT 1846 1846 . . 1851 1856 + AsuII TTCGAA 1852 1854 . . 1852 1855 + TaqI TCGA 1852 1854 . . 1872 1881 + XmnI GAANNNNTTC 1876 1876 . . 1875 1880 + SspI AATATT 1877 1877 . . 1889 1892 + CviRI* TGCA 1890 1890 . . 1920 1923 + SetI ASST 1923 1919 . . 1920 1923 + CviJI RGCY 1921 1921 . . 1920 1923 + AluI AGCT 1921 1921 . . 1946 1950 + Tsp4CI* ACNGT 1948 1947 . . 1958 1961 + CviJI RGCY 1959 1959 . . 1960 1964 + DdeI CTNAG 1960 1963 . . 1960 1964 - BspCNI CTCAG 1952 1950 . . 1960 1964 - BseMII CTCAG 1951 1949 . . 1962 1966 - BsmAI GTCTC 1956 1960 . . 1974 1977 + SetI ASST 1977 1973 . . 1974 1980 + SexAI ACCWGGT 1974 1979 . . 1975 1979 + BssKI CCNGG 1974 1979 . . 1975 1979 + BseBI CCWGG 1976 1977 . . 1975 1979 + ScrFI CCNGG 1976 1977 . . 1975 1979 + EcoRII CCWGG 1974 1979 . . 1979 1982 + Csp6I GTAC 1979 1981 . . 1979 1982 + RsaI GTAC 1980 1980 . . 1981 1985 + Tsp4CI* ACNGT 1983 1982 . . 1998 2003 + AhaIII* TTTAAA 2000 2000 . . 1999 2002 + MseI TTAA 1999 2001 . . 2007 2011 + TfiI GAWTC 2007 2010 . . 2007 2011 + HinfI GANTC 2007 2010 . . 2013 2016 + TspEI AATT 2012 2016 . . 2026 2031 + VspI ATTAAT 2027 2029 . . 2023 2027 - TspDTI ATGAA 2013 2011 . . 2027 2030 + MseI TTAA 2027 2029 . . 2036 2039 + TspEI AATT 2035 2039 . . 2037 2042 + VspI ATTAAT 2038 2040 . . 2038 2041 + MseI TTAA 2038 2040 . . 2054 2059 + CfrI YGGCCR 2054 2058 . . 2055 2058 + CviJI RGCY 2056 2056 . . 2055 2058 + HaeIII GGCC 2056 2056 . . 2067 2071 + Tsp4CI* ACNGT 2069 2068 . . 2068 2073 + McrI* CGRYCG 2071 2069 . . 2071 2074 + TaqI TCGA 2071 2073 . . 2073 2077 + TfiI GAWTC 2073 2076 . . 2073 2077 + HinfI GANTC 2073 2076 . . 2089 2093 - BccI CCATC 2083 2084 . . 2095 2100 + AhaIII* TTTAAA 2097 2097 . . 2096 2099 + MseI TTAA 2096 2098 . . 2100 2103 + SetI ASST 2103 2099 . . 2101 2105 + BssKI CCNGG 2100 2105 . . 2101 2105 + BseBI CCWGG 2102 2103 . . 2101 2105 + ScrFI CCNGG 2102 2103 . . 2101 2105 + EcoRII CCWGG 2100 2105 . . 2103 2108 + BalI TGGCCA 2105 2105 . . 2103 2108 + CfrI YGGCCR 2103 2107 . . 2104 2107 + CviJI RGCY 2105 2105 . . 2104 2107 + HaeIII GGCC 2105 2105 . . 2106 2119 - AjuI GAANNNNNNNTTGG 2131 2126 2099 2094 2139 2144 + ApoI RAATTY 2139 2143 . . 2140 2143 + TspEI AATT 2139 2143 . . 2142 2147 + AhaIII* TTTAAA 2144 2144 . . 2143 2146 + MseI TTAA 2143 2145 . . 2149 2153 + Hpy188I TCNGA 2151 2150 . . 2148 2153 - MmeI TCCRAC 2129 2127 . . 2163 2166 + SetI ASST 2166 2162 . . 2163 2166 + CviJI RGCY 2164 2164 . . 2163 2166 + AluI AGCT 2164 2164 . . 2164 2174 + MwoI GCNNNNNNNGC 2170 2167 . . 2172 2175 + CviRI* TGCA 2173 2173 . . 2179 2184 + BsaAI YACGTR 2181 2181 . . 2180 2183 + TaiI ACGT 2183 2179 . . 2180 2183 + SetI ASST 2183 2179 . . 2180 2183 + MaeII ACGT 2180 2182 . . 2192 2195 + Csp6I GTAC 2192 2194 . . 2192 2195 + RsaI GTAC 2193 2193 . . 2194 2198 + BceAI ACGGC 2210 2212 . . 2203 2212 + MslI CAYNNNNRTG 2207 2207 . . 2203 2212 + OliI CACNNNNGTG 2207 2207 . . 2205 2210 + DsaI* CCRYGG 2205 2209 . . 2205 2210 + StyI CCWWGG 2205 2209 . . 2205 2210 + NcoI CCATGG 2205 2209 . . 2205 2210 + SecI* CCNNGG 2205 2209 . . 2206 2209 + FatI CATG 2205 2209 . . 2206 2209 + NlaIII CATG 2209 2205 . . 2206 2209 + CviAII CATG 2206 2208 . . 2202 2206 - HphI GGTGA 2194 2193 . . 2209 2213 + HphI GGTGA 2221 2220 . . 2240 2243 + CviJI RGCY 2241 2241 . . 2241 2246 + Cac8I GCNNGC 2243 2243 . . 2241 2246 + BmtI GCTAGC 2245 2241 . . 2241 2246 + NheI GCTAGC 2241 2245 . . 2242 2245 + MaeI CTAG 2242 2244 . . 2244 2247 + CviJI RGCY 2245 2245 . . 2247 2261 + XcmI CCANNNNNNNNNTGG 2254 2253 . . 2260 2265 + NlaIV GGNNCC 2262 2262 . . 2261 2265 + BmgT120I GGNCC 2262 2263 . . 2261 2265 + AvaII GGWCC 2261 2264 . . 2261 2265 + AsuI* GGNCC 2261 2264 . . 2263 2268 + MmeI TCCRAC 2288 2286 . . 2269 2274 + SspI AATATT 2271 2271 . . 2286 2289 + SetI ASST 2289 2285 . . 2291 2295 + Tsp4CI* ACNGT 2293 2292 . . 2303 2306 + Csp6I GTAC 2303 2305 . . 2303 2306 + RsaI GTAC 2304 2304 . . 2310 2313 + CviJI RGCY 2311 2311 . . 2313 2319 + TspRI CASTGNN 2319 2310 . . 2314 2318 + BsrI ACTGG 2319 2317 . . 2328 2331 + CviRI* TGCA 2329 2329 . . 2329 2339 + MwoI GCNNNNNNNGC 2335 2332 . . 2329 2333 + BlsI GCNGC 2331 2330 . . 2329 2333 + BisI GCNGC 2330 2331 . . 2329 2333 + BbvI GCAGC 2341 2345 . . 2329 2333 + TseI GCWGC 2329 2332 . . 2330 2335 + NspBII* CMGCKG 2332 2332 . . 2326 2330 - SfaNI GCATC 2316 2320 . . 2332 2336 + TauI GCSGC 2335 2332 . . 2332 2336 + BlsI GCNGC 2334 2333 . . 2332 2336 + BisI GCNGC 2333 2334 . . 2335 2339 + BlsI GCNGC 2337 2336 . . 2335 2339 + BisI GCNGC 2336 2337 . . 2335 2339 + BbvI GCAGC 2347 2351 . . 2335 2339 + TseI GCWGC 2335 2338 . . 2332 2335 - AciI CCGC 2332 2334 . . 2357 2360 + TspEI AATT 2356 2360 . . 2366 2369 + TspEI AATT 2365 2369 . . 2368 2371 + MseI TTAA 2368 2370 . . 2385 2389 - HphI GGTGA 2377 2376 . . 2390 2395 - EcoP15I CAGCAG 2362 2364 . . 2398 2401 + DpnI GATC 2399 2399 . . 2398 2401 + BstKTI GATC 2400 2398 . . 2398 2401 + MboI GATC 2397 2401 . . 2398 2402 - BinI* GGATC 2392 2393 . . 2422 2426 + MboII GAAGA 2434 2433 . . 2434 2438 + Tsp4CI* ACNGT 2436 2435 . . 2435 2441 + TspRI CASTGNN 2441 2432 . . 2439 2444 + NlaIV GGNNCC 2441 2441 . . 2441 2444 + CviJI RGCY 2442 2442 . . 2439 2449 - BsaXI ACNNNNNCTCC 2461 2458 2431 2428 2439 2450 - TstI CACNNNNNNTCC 2463 2458 2431 2426 2460 2463 + TspEI AATT 2459 2463 . . 2463 2467 - MboII GAAGA 2455 2454 . . 2472 2476 + TfiI GAWTC 2472 2475 . . 2472 2476 + HinfI GANTC 2472 2475 . . 2479 2484 + Hpy166II GTNNAC 2481 2481 . . 2479 2484 + HpaI GTTAAC 2481 2481 . . 2479 2484 + HindII GTYRAC 2481 2481 . . 2480 2483 + MseI TTAA 2480 2482 . . 2483 2487 + Tsp4CI* ACNGT 2485 2484 . . 2489 2493 + DdeI CTNAG 2489 2492 . . 2489 2493 - BspCNI CTCAG 2481 2479 . . 2489 2493 - BseMII CTCAG 2480 2478 . . 2491 2494 - MnlI CCTC 2484 2483 . . 2488 2494 - Bpu10I CCTNAGC 2489 2492 . . 2488 2494 - BbvCI CCTCAGC 2489 2492 . . 2502 2508 + TspRI CASTGNN 2508 2499 . . 2503 2507 + BsrI ACTGG 2508 2506 . . 2506 2509 + CviJI RGCY 2507 2507 . . 2508 2513 + Eco57MI CTGRAG 2529 2527 . . 2508 2513 + GsuI CTGGAG 2529 2527 . . 2512 2517 + TatI WGTACW 2512 2516 . . 2512 2517 + ScaI AGTACT 2514 2514 . . 2513 2516 + Csp6I GTAC 2513 2515 . . 2513 2516 + RsaI GTAC 2514 2514 . . 2524 2527 + MnlI CCTC 2534 2533 . . 2544 2547 + TspEI AATT 2543 2547 . . 2546 2551 + PsiI TTATAA 2548 2548 . . 2566 2569 + MseI TTAA 2566 2568 . . 2569 2573 + TspDTI ATGAA 2584 2582 . . 2574 2578 + Hpy99I CGWCG 2578 2573 . . 2578 2583 + BseRI GAGGAG 2593 2591 . . 2578 2581 - MnlI CCTC 2571 2570 . . 2582 2585 + SetI ASST 2585 2581 . . 2582 2585 + CviJI RGCY 2583 2583 . . 2582 2585 + AluI AGCT 2583 2583 . . 2584 2589 + SmlI CTYRAG 2584 2588 . . 2584 2589 + Bce83I* CTTGAG 2605 2603 . . 2593 2598 + EcoT22I ATGCAT 2597 2593 . . 2594 2597 + CviRI* TGCA 2595 2595 . . 2595 2599 + SfaNI GCATC 2604 2608 . . 2599 2604 + DsaI* CCRYGG 2599 2603 . . 2599 2604 + SecI* CCNNGG 2599 2603 . . 2596 2600 - FokI GGATG 2582 2586 . . 2596 2600 - BseGI GGATG 2595 2593 . . 2598 2602 - TspGWI ACGGA 2588 2586 . . 2611 2615 + FokI GGATG 2624 2628 . . 2611 2615 + BseGI GGATG 2617 2615 . . 2615 2619 + BmgT120I GGNCC 2616 2617 . . 2615 2619 + AvaII GGWCC 2615 2618 . . 2615 2619 + AsuI* GGNCC 2615 2618 . . 2612 2616 - BccI CCATC 2606 2607 . . 2617 2622 + Cfr10I RCCGGY 2617 2621 . . 2617 2622 + BetI* WCCGGW 2617 2621 . . 2617 2622 + AgeI ACCGGT 2617 2621 . . 2618 2621 + HpaII CCGG 2618 2620 . . 2621 2624 + Csp6I GTAC 2621 2623 . . 2621 2624 + RsaI GTAC 2622 2622 . . 2629 2633 + MboII GAAGA 2641 2640 . . 2634 2637 + TspEI AATT 2633 2637 . . 2639 2642 + CviJI RGCY 2640 2640 . . 2641 2644 + MnlI CCTC 2651 2650 . . 2648 2652 + MaeIII GTNAC 2647 2652 . . 2658 2661 + Csp6I GTAC 2658 2660 . . 2658 2661 + RsaI GTAC 2659 2659 . . 2660 2664 + Tsp4CI* ACNGT 2662 2661 . . 2676 2679 + TspEI AATT 2675 2679 . . 2687 2690 + TaiI ACGT 2690 2686 . . 2687 2690 + SetI ASST 2690 2686 . . 2687 2690 + MaeII ACGT 2687 2689 . . 2680 2690 - BsaXI ACNNNNNCTCC 2702 2699 2672 2669 2681 2691 - Hin4I GAYNNNNNVTC 2704 2699 2672 2667 2691 2695 + DdeI CTNAG 2691 2694 . . 2702 2706 + DdeI CTNAG 2702 2705 . . 2702 2706 - BspCNI CTCAG 2694 2692 . . 2702 2706 - BseMII CTCAG 2693 2691 . . 2709 2714 + Cac8I GCNNGC 2711 2711 . . 2710 2724 + XcmI CCANNNNNNNNNTGG 2717 2716 . . 2712 2715 + CviJI RGCY 2713 2713 . . 2714 2724 + BsiYI* CCNNNNNNNGG 2720 2717 . . 2714 2724 + PflMI CCANNNNNTGG 2720 2717 . . 2728 2731 + TaqI TCGA 2728 2730 . . 2737 2747 + FalI AAGNNNNNCTT 2728 2723 2760 2755 2741 2746 + TatI WGTACW 2741 2745 . . 2741 2746 + ScaI AGTACT 2743 2743 . . 2742 2745 + Csp6I GTAC 2742 2744 . . 2742 2745 + RsaI GTAC 2743 2743 . . 2737 2747 - FalI AAGNNNNNCTT 2760 2755 2728 2723 2754 2757 + SetI ASST 2757 2753 . . 2758 2761 + MseI TTAA 2758 2760 . . 2765 2769 + MaeIII GTNAC 2764 2769 . . 2762 2765 - AciI CCGC 2762 2764 . . 2782 2787 + SecI* CCNNGG 2782 2786 . . 2783 2787 + BssKI CCNGG 2782 2787 . . 2783 2787 + BseBI CCWGG 2784 2785 . . 2783 2787 + ScrFI CCNGG 2784 2785 . . 2783 2787 + EcoRII CCWGG 2782 2787 . . 2786 2790 + BinI* GGATC 2794 2795 . . 2787 2790 + DpnI GATC 2788 2788 . . 2787 2790 + BstKTI GATC 2789 2787 . . 2787 2790 + MboI GATC 2786 2790 . . 2800 2805 + BseRI GAGGAG 2815 2813 . . 2800 2803 - MnlI CCTC 2793 2792 . . 2805 2809 + BlsI GCNGC 2807 2806 . . 2805 2809 + BisI GCNGC 2806 2807 . . 2805 2809 + BbvI GCAGC 2817 2821 . . 2805 2809 + TseI GCWGC 2805 2808 . . 2824 2829 + ApoI RAATTY 2824 2828 . . 2825 2828 + TspEI AATT 2824 2828 . . 2827 2831 - TspDTI ATGAA 2817 2815 . . 2844 2847 + SetI ASST 2847 2843 . . 2845 2851 + SauI* CCTNAGG 2846 2849 . . 2846 2850 + DdeI CTNAG 2846 2849 . . 2842 2846 - HphI GGTGA 2834 2833 . . 2846 2850 - BspCNI CTCAG 2838 2836 . . 2846 2850 - BseMII CTCAG 2837 2835 . . 2848 2851 - MnlI CCTC 2841 2840 . . 2856 2859 + CviJI RGCY 2857 2857 . . 2855 2860 - TsoI TARCCA 2845 2843 . . 2874 2877 + SetI ASST 2877 2873 . . 2882 2886 + TspDTI ATGAA 2897 2895 . . 2890 2895 + AhaIII* TTTAAA 2892 2892 . . 2891 2894 + MseI TTAA 2891 2893 . . 2898 2901 + TspEI AATT 2897 2901 . . 2917 2921 + TspDTI ATGAA 2932 2930 . . 2935 2938 + DpnI GATC 2936 2936 . . 2935 2938 + BstKTI GATC 2937 2935 . . 2935 2938 + MboI GATC 2934 2938 . . 2938 2941 + MnlI CCTC 2948 2947 . . 2935 2939 - BinI* GGATC 2929 2930 . . 2951 2956 + Hpy178III* TCNNGA 2952 2954 . . 2957 2962 + TatI WGTACW 2957 2961 . . 2958 2961 + Csp6I GTAC 2958 2960 . . 2958 2961 + RsaI GTAC 2959 2959 . . 2962 2965 + TspEI AATT 2961 2965 . . 2965 2969 - TspGWI ACGGA 2955 2953 . . 2972 2976 + TspDTI ATGAA 2987 2985 . . 2976 2981 + SspI AATATT 2978 2978 . . 2980 2983 + MseI TTAA 2980 2982 . . 2986 2991 + ApoI RAATTY 2986 2990 . . 2987 2990 + TspEI AATT 2986 2990 . . 2996 2999 + TaiI ACGT 2999 2995 . . 2996 2999 + SetI ASST 2999 2995 . . 2996 2999 + MaeII ACGT 2996 2998 . . 2999 3003 + Hpy188I TCNGA 3001 3000 . . 3004 3007 + MaeI CTAG 3004 3006 . . 3031 3036 + NdeI CATATG 3032 3034 . . 3043 3046 + SetI ASST 3046 3042 . . 3045 3049 + MaeIII GTNAC 3044 3049 . . 3048 3052 + Tsp4CI* ACNGT 3050 3049 . . 3049 3055 + TspRI CASTGNN 3055 3046 . . 3056 3059 + SetI ASST 3059 3055 . . 3055 3058 - MnlI CCTC 3048 3047 . . 3061 3071 + BplI GAGNNNNNCTC 3052 3047 3084 3079 3061 3071 - BplI GAGNNNNNCTC 3084 3079 3052 3047 3082 3087 + ApoI RAATTY 3082 3086 . . 3083 3086 + TspEI AATT 3082 3086 . . 3089 3092 + MseI TTAA 3089 3091 . . 3111 3116 + Hpy166II GTNNAC 3113 3113 . . 3111 3116 + HpaI GTTAAC 3113 3113 . . 3111 3116 + HindII GTYRAC 3113 3113 . . 3112 3115 + MseI TTAA 3112 3114 . . 3115 3119 + Tsp4CI* ACNGT 3117 3116 . . 3118 3122 + Tsp45I GTSAC 3117 3122 . . 3118 3122 + MaeIII GTNAC 3117 3122 . . 3119 3123 - HphI GGTGA 3111 3110 . . 3128 3131 + CviJI RGCY 3129 3129 . . 3130 3135 + StyI CCWWGG 3130 3134 . . 3130 3135 + SecI* CCNNGG 3130 3134 . . 3130 3135 + AvrII CCTAGG 3130 3134 . . 3131 3134 + MaeI CTAG 3131 3133 . . 3154 3159 + SmlI CTYRAG 3154 3158 . . 3154 3159 + Bce83I* CTTGAG 3175 3173 . . 3159 3164 + ApoI RAATTY 3159 3163 . . 3160 3163 + TspEI AATT 3159 3163 . . 3167 3172 + NlaIV GGNNCC 3169 3169 . . 3179 3182 + MseI TTAA 3179 3181 . . 3189 3192 + SetI ASST 3192 3188 . . 3187 3191 - Hin4II* CCTTC 3181 3180 . . 3191 3196 + Hpy166II GTNNAC 3193 3193 . . 3196 3206 + BsiYI* CCNNNNNNNGG 3202 3199 . . 3196 3206 + PflMI CCANNNNNTGG 3202 3199 . . 3225 3228 + SetI ASST 3228 3224 . . 3225 3228 + CviJI RGCY 3226 3226 . . 3225 3228 + AluI AGCT 3226 3226 . . 3234 3237 + TspEI AATT 3233 3237 . . 3236 3239 + MseI TTAA 3236 3238 . . 3240 3244 + Hpy99I CGWCG 3244 3239 . . 3242 3245 + TaiI ACGT 3245 3241 . . 3242 3245 + SetI ASST 3245 3241 . . 3242 3245 + MaeII ACGT 3242 3244 . . 3251 3254 + Hin6I GCGC 3251 3253 . . 3251 3254 + GlaI GCGC 3252 3252 . . 3251 3254 + HhaI GCGC 3253 3251 . . 3259 3263 + Hpy188I TCNGA 3261 3260 . . 3263 3267 + TspDTI ATGAA 3278 3276 . . 3265 3269 + MboII GAAGA 3277 3276 . . 3268 3272 + MboII GAAGA 3280 3279 . . 3272 3276 + TspDTI ATGAA 3287 3285 . . 3274 3278 + MboII GAAGA 3286 3285 . . 3281 3286 + Hpy166II GTNNAC 3283 3283 . . 3289 3293 + MboII GAAGA 3301 3300 . . 3305 3310 + PsiI TTATAA 3307 3307 . . 3313 3317 + BmgT120I GGNCC 3314 3315 . . 3313 3317 + AvaII GGWCC 3313 3316 . . 3313 3317 + AsuI* GGNCC 3313 3316 . . 3315 3320 + Hpy178III* TCNNGA 3316 3318 . . 3317 3322 + Eco57MI CTGRAG 3338 3336 . . 3317 3322 + Eco57I CTGAAG 3338 3336 . . 3319 3323 + MboII GAAGA 3331 3330 . . 3335 3340 + NdeI CATATG 3336 3338 . . 3338 3342 + TspDTI ATGAA 3353 3351 . . 3341 3346 + SspI AATATT 3343 3343 . . 3374 3379 + StyI CCWWGG 3374 3378 . . 3374 3379 + SecI* CCNNGG 3374 3378 . . 3383 3388 + EcoRV GATATC 3385 3385 . . 3392 3395 + SetI ASST 3395 3391 . . 3392 3395 + CviJI RGCY 3393 3393 . . 3392 3395 + AluI AGCT 3393 3393 . . 3418 3422 - BsmAI GTCTC 3412 3416 . . 3417 3427 - BsaXI ACNNNNNCTCC 3439 3436 3409 3406 3426 3431 - MmeI TCCRAC 3407 3405 . . 3439 3442 + MseI TTAA 3439 3441 . . 3449 3452 + AciI CCGC 3449 3451 . . 3450 3453 + FnuDII* CGCG 3451 3451 . . 3468 3474 + TspRI CASTGNN 3474 3465 . . 3480 3483 + CviJI RGCY 3481 3481 . . 3477 3481 - Hin4II* CCTTC 3471 3470 . . 3488 3491 + SetI ASST 3491 3487 . . 3487 3490 - MnlI CCTC 3480 3479 . . 3494 3499 + EcoRV GATATC 3496 3496 . . 3498 3503 + Hpy178III* TCNNGA 3499 3501 . . 3502 3507 + ApoI RAATTY 3502 3506 . . 3503 3506 + TspEI AATT 3502 3506 . . 3509 3512 + CviRI* TGCA 3510 3510 . . 3510 3521 + AlfI GCANNNNNNTGC 3499 3497 3533 3531 3519 3522 + CviRI* TGCA 3520 3520 . . 3517 3521 - SfaNI GCATC 3507 3511 . . 3510 3521 - AlfI GCANNNNNNTGC 3533 3531 3499 3497 3525 3528 + SetI ASST 3528 3524 . . 3525 3528 + CviJI RGCY 3526 3526 . . 3525 3528 + AluI AGCT 3526 3526 . . 3526 3531 + Cac8I GCNNGC 3528 3528 . . 3529 3532 + FnuDII* CGCG 3530 3530 . . 3530 3533 - AciI CCGC 3530 3532 . . 3544 3554 + BsiYI* CCNNNNNNNGG 3550 3547 . . 3545 3555 + BsiYI* CCNNNNNNNGG 3551 3548 . . 3567 3570 + SetI ASST 3570 3566 . . 3583 3588 + HindIII AAGCTT 3583 3587 . . 3584 3587 + SetI ASST 3587 3583 . . 3584 3587 + CviJI RGCY 3585 3585 . . 3584 3587 + AluI AGCT 3585 3585 . . 3589 3594 + Hpy166II GTNNAC 3591 3591 . . 3589 3594 + HindII GTYRAC 3591 3591 . . 3594 3598 + Hpy99I CGWCG 3598 3593 . . 3598 3602 + MboII GAAGA 3610 3609 . . 3598 3603 + BbvII* GAAGAC 3605 3609 . . 3610 3614 + BccI CCATC 3618 3619 . . 3631 3636 + TatI WGTACW 3631 3635 . . 3631 3636 + ScaI AGTACT 3633 3633 . . 3632 3635 + Csp6I GTAC 3632 3634 . . 3632 3635 + RsaI GTAC 3633 3633 . . 3638 3641 + MaeI CTAG 3638 3640 . . 3654 3657 + TspEI AATT 3653 3657 . . 3656 3659 + MseI TTAA 3656 3658 . . 3664 3667 - MnlI CCTC 3657 3656 . . 3672 3677 + ApoI RAATTY 3672 3676 . . 3673 3676 + TspEI AATT 3672 3676 . . 3702 3707 + SpeI ACTAGT 3702 3706 . . 3703 3706 + MaeI CTAG 3703 3705 . . 3706 3710 + MaeIII GTNAC 3705 3710 . . 3707 3712 + TsoI TARCCA 3723 3721 . . 3720 3725 + NarI GGCGCC 3721 3723 . . 3720 3725 + KasI GGCGCC 3720 3724 . . 3720 3725 + DinI GGCGCC 3722 3722 . . 3720 3725 + HaeII RGCGCY 3724 3720 . . 3720 3725 + NlaIV GGNNCC 3722 3722 . . 3720 3725 + AcyI GRCGYC 3721 3723 . . 3720 3725 + HgiCI* GGYRCC 3720 3724 . . 3721 3724 + Hin6I GCGC 3721 3723 . . 3721 3724 + GlaI GCGC 3722 3722 . . 3721 3724 + HhaI GCGC 3723 3721 . . 3734 3737 + MseI TTAA 3734 3736 . . 3751 3755 + Hin4II* CCTTC 3761 3760 . . 3760 3764 + Hpy188I TCNGA 3762 3761 . . 3775 3779 + MboII GAAGA 3787 3786 . . 3778 3782 + MboII GAAGA 3790 3789 . . 3781 3785 + MboII GAAGA 3793 3792 . . 3781 3786 - Ksp632I* CTCTTC 3776 3779 . . 3786 3791 + BsmI GAATGC 3792 3790 . . 3793 3797 + Hin4II* CCTTC 3803 3802 . . 3796 3800 - MboII GAAGA 3788 3787 . . 3801 3806 + BsiI* CACGAG 3801 3805 . . 3808 3812 - MboII GAAGA 3800 3799 . . 3811 3815 - MboII GAAGA 3803 3802 . . 3821 3824 + TaqI TCGA 3821 3823 . . 3835 3839 + MboII GAAGA 3847 3846 . . 3847 3850 - MnlI CCTC 3840 3839 . . 3856 3860 + DdeI CTNAG 3856 3859 . . 3856 3860 - BspCNI CTCAG 3848 3846 . . 3856 3860 - BseMII CTCAG 3847 3845 . . 3867 3872 + AsuII TTCGAA 3868 3870 . . 3868 3871 + TaqI TCGA 3868 3870 . . 3877 3882 + AhaIII* TTTAAA 3879 3879 . . 3878 3881 + MseI TTAA 3878 3880 . . 3885 3888 + TspEI AATT 3884 3888 . . 3920 3923 + AciI CCGC 3920 3922 . . 3928 3931 + TaqI TCGA 3928 3930 . . 3930 3934 - Hin4II* CCTTC 3924 3923 . . 3959 3964 + TsoI TARCCA 3975 3973 . . 3960 3963 + CviJI RGCY 3961 3961 . . 3983 3986 + AciI CCGC 3983 3985 . . 3995 4000 + PvuII CAGCTG 3997 3997 . . 3995 4000 + NspBII* CMGCKG 3997 3997 . . 3996 3999 + SetI ASST 3999 3995 . . 3996 3999 + CviJI RGCY 3997 3997 . . 3996 3999 + AluI AGCT 3997 3997 . . 4005 4008 + SetI ASST 4008 4004 . . 4010 4013 + TspEI AATT 4009 4013 . . 4013 4018 + BalI TGGCCA 4015 4015 . . 4013 4018 + CfrI YGGCCR 4013 4017 . . 4014 4017 + CviJI RGCY 4015 4015 . . 4014 4017 + HaeIII GGCC 4015 4015 . . 4022 4025 + CviJI RGCY 4023 4023 . . 4028 4033 + Hpy178III* TCNNGA 4029 4031 . . 4024 4028 - BsrI ACTGG 4024 4022 . . 4060 4064 + HphI GGTGA 4072 4071 . . 4070 4073 + SetI ASST 4073 4069 . . 4072 4075 + MaeI CTAG 4072 4074 . . 4076 4081 + ApoI RAATTY 4076 4080 . . 4077 4080 + TspEI AATT 4076 4080 . . 4084 4088 + TfiI GAWTC 4084 4087 . . 4084 4088 + HinfI GANTC 4084 4087 . . 4086 4090 - TspDTI ATGAA 4076 4074 . . 4092 4098 + TspRI CASTGNN 4098 4089 . . 4096 4099 + DpnI GATC 4097 4097 . . 4096 4099 + BstKTI GATC 4098 4096 . . 4096 4099 + MboI GATC 4095 4099 . . 4098 4103 + Hpy178III* TCNNGA 4099 4101 . . 4102 4106 + MboII GAAGA 4114 4113 . . 4118 4122 + TspDTI ATGAA 4133 4131 . . 4120 4124 + MboII GAAGA 4132 4131 . . 4126 4130 - SfaNI GCATC 4116 4120 . . 4142 4145 + DpnI GATC 4143 4143 . . 4142 4145 + BstKTI GATC 4144 4142 . . 4142 4145 + MboI GATC 4141 4145 . . 4149 4153 - BsmAI GTCTC 4143 4147 . . 4157 4160 + DpnI GATC 4158 4158 . . 4157 4160 + BstKTI GATC 4159 4157 . . 4157 4160 + MboI GATC 4156 4160 . . 4160 4164 + Hpy99I CGWCG 4164 4159 . . 4162 4165 + TaqI TCGA 4162 4164 . . 4162 4167 + Hpy178III* TCNNGA 4163 4165 . . 4167 4170 + SetI ASST 4170 4166 . . 4167 4170 + CviJI RGCY 4168 4168 . . 4167 4170 + AluI AGCT 4168 4168 . . 4168 4172 + BlsI GCNGC 4170 4169 . . 4168 4172 + BisI GCNGC 4169 4170 . . 4168 4172 + TseI GCWGC 4168 4171 . . 4172 4176 + Hpy99I CGWCG 4176 4171 . . 4168 4172 - BbvI GCAGC 4155 4159 . . 4171 4175 - HgaI GACGC 4160 4165 . . 4176 4181 + NlaIV GGNNCC 4178 4178 . . 4181 4185 + DdeI CTNAG 4181 4184 . . 4193 4196 + TaiI ACGT 4196 4192 . . 4193 4196 + SetI ASST 4196 4192 . . 4193 4196 + MaeII ACGT 4193 4195 . . 4208 4211 + TspEI AATT 4207 4211 . . 4213 4217 + Hpy188I TCNGA 4215 4214 . . 4220 4224 + Tsp4CI* ACNGT 4222 4221 . . 4225 4229 - TspDTI ATGAA 4215 4213 . . 4229 4232 + TaqI TCGA 4229 4231 . . 4231 4235 + MboII GAAGA 4243 4242 . . 4238 4242 + TspDTI ATGAA 4253 4251 . . 4240 4244 + MboII GAAGA 4252 4251 . . 4240 4245 - Ksp632I* CTCTTC 4235 4238 . . 4243 4246 - MnlI CCTC 4236 4235 . . 4251 4256 + StyI CCWWGG 4251 4255 . . 4251 4256 + SecI* CCNNGG 4251 4255 . . 4255 4259 + BinI* GGATC 4263 4264 . . 4256 4259 + DpnI GATC 4257 4257 . . 4256 4259 + BstKTI GATC 4258 4256 . . 4256 4259 + MboI GATC 4255 4259 . . 4258 4262 + Hpy188I TCNGA 4260 4259 . . 4276 4281 - Ksp632I* CTCTTC 4271 4274 . . 4279 4282 - MnlI CCTC 4272 4271 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 940 3347 # # AciI: # [CCGC] # 63 65 81 122 211 304 # 390 430 478 601 687 855 # # AcyI: # [GRCGYC] # 566 3721 # # AgeI: # [ACCGGT] # 12 1670 2605 # # AhaIII*: # [TTTAAA] # 47 97 408 748 987 2000 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 32 344 792 899 # 2156 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 754 3499 # # AluI: # [AGCT] # 15 59 66 111 119 133 # 167 171 181 189 243 290 # 330 412 419 643 739 # # ApoI: # [RAATTY] # 9 13 41 65 77 96 # 108 162 167 170 191 211 # 272 343 359 404 422 492 # 685 # # AsuI*: # [GGNCC] # 354 562 571 698 974 1128 # # AsuII: # [TTCGAA] # 419 1852 2016 # # AvaII: # [GGWCC] # 354 562 571 698 974 1128 # # AvrII: # [CCTAGG] # 1157 3130 # # BaeI: # [ACNNNNGTAYC] # 33 33 152 747 3322 # # BalI: # [TGGCCA] # 272 497 1608 1910 # # BbvCI: # [CCTCAGC] # 269 1798 2220 # # BbvI: # [GCAGC] # 6 22 98 132 470 1338 # 2221 # # BbvII*: # [GAAGAC] # 682 3605 # # BccI: # [CCATC] # 49 126 243 272 290 342 # 375 386 523 669 1012 # # Bce83I*: # [CTTGAG] # 86 570 1112 2519 # # BceAI: # [ACGGC] # 2077 2210 # # BclI: # [TGATCA] # 107 1458 2722 # # BdaI: # [TGANNNNNNTCA] # 0 0 16 34 4237 # # BetI*: # [WCCGGW] # 12 1670 2605 # # BglII: # [AGATCT] # 483 3804 # # BinI*: # [GGATC] # 24 135 226 402 1334 2166 # # BisI: # [GCNGC] # 3 3 3 100 109 118 # 470 600 1363 1518 # # BlsI: # [GCNGC] # 3 3 3 100 110 117 # 470 600 1363 1518 # # BmgT120I: # [GGNCC] # 354 562 572 698 973 1128 # # BmtI: # [GCTAGC] # 2042 2245 # # BplI: # [GAGNNNNNCTC] # 0 0 32 1203 3052 # # Bpu10I: # [CCTNAGC] # 269 1798 2220 # # BsaAI: # [YACGTR] # 2106 2181 # # BsaBI: # [GATNNNNATC] # 32 1548 2707 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 211 707 848 # 2431 # # BseBI: # [CCWGG] # 126 173 336 682 1467 1503 # # BseGI: # [GGATG] # 22 379 1670 2216 # # BseMII: # [CTCAG] # 144 213 260 440 529 1010 # 1691 # # BseRI: # [GAGGAG] # 222 1472 2593 # # BsiI*: # [CACGAG] # 486 3801 # # BsiYI*: # [CCNNNNNNNGG] # 1 139 348 482 736 1284 # 1297 # # BslFI: # [GGGAC] # 711 1119 2457 # # BsmAI: # [GTCTC] # 127 144 731 1456 1829 # # BsmI: # [GAATGC] # 495 1729 2063 # # BspCNI: # [CTCAG] # 144 213 261 439 529 1010 # 1691 # # BspHI: # [TCATGA] # 16 1568 2703 # # BspMI: # [ACCTGC] # 1366 2921 # # BsrDI: # [GCAATG] # 121 799 3367 # # BsrI: # [ACTGG] # 56 189 263 361 872 1030 # 1516 # # BssKI: # [CCNGG] # 126 171 336 682 1467 1505 # # BstKTI: # [GATC] # 6 10 15 29 46 99 # 104 107 148 234 252 389 # 712 975 1161 # # BtgZI: # [GCGATG] # 1501 2786 # # Cac8I: # [GCNNGC] # 468 759 817 2243 # # Cfr10I: # [RCCGGY] # 12 1291 1314 1670 # # CfrI: # [YGGCCR] # 49 274 495 1559 1910 # # ClaI: # [ATCGAT] # 13 238 4036 # # Csp6I: # [GTAC] # 21 37 57 84 108 111 # 169 210 213 216 309 651 # 655 674 772 # # CviAII: # [CATG] # 13 16 121 144 263 405 # 621 623 2081 # # CviJI: # [RGCY] # 4 8 15 16 18 30 # 36 38 39 45 49 50 # 56 57 59 59 65 66 # 66 73 76 77 88 97 # 97 103 111 114 119 131 # 142 144 145 167 272 290 # 330 376 559 # # CviRI*: # [TGCA] # 10 29 114 156 156 161 # 266 283 294 338 354 444 # 767 915 # # DdeI: # [CTNAG] # 11 68 106 144 168 170 # 201 202 208 325 529 1010 # 1145 # # DinI: # [GGCGCC] # 565 3722 # # DpnI: # [GATC] # 6 10 15 30 46 99 # 104 107 148 233 252 389 # 712 975 1161 # # DraII: # [RGGNCCY] # 1133 3154 # # DraIII: # [CACNNNGTG] # 1423 2864 # # DrdI: # [GACNNNNNNGTC] # 700 3587 # # DsaI*: # [CCRYGG] # 394 932 1273 1688 # # EciI: # [GGCGGA] # 1344 2943 # # Eco31I: # [GGTCTC] # 127 4160 # # Eco57I: # [CTGAAG] # 949 3338 # # Eco57MI: # [CTGRAG] # 809 949 2529 # # EcoP15I: # [CAGCAG] # 1925 2362 # # EcoRI: # [GAATTC] # 452 463 757 2615 # # EcoRII: # [CCWGG] # 126 171 336 682 1467 1505 # # EcoRV: # [GATATC] # 33 63 111 150 642 765 # 791 1732 # # EcoT22I: # [ATGCAT] # 1690 2597 # # EspI*: # [GCTNAGC] # 437 3850 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1527 2728 # # FatI: # [CATG] # 13 16 121 143 263 405 # 621 623 2082 # # FnuDII*: # [CGCG] # 79 757 1477 1974 # # FokI: # [GGATG] # 42 366 1663 2216 # # GlaI: # [GCGC] # 21 470 565 1211 2020 # # GsuI: # [CTGGAG] # 1758 2529 # # HaeII: # [RGCGCY] # 23 563 3701 # # HaeIII: # [GGCC] # 49 272 497 1559 1910 # # HgaI: # [GACGC] # 127 1222 2938 # # HgiCI*: # [GGYRCC] # 132 567 808 2780 # # HhaI: # [GCGC] # 22 470 564 1211 2020 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 26 32 32 32 # 32 32 32 199 348 376 # 679 884 1583 # # Hin4II*: # [CCTTC] # 30 42 121 290 290 363 # 561 748 1842 # # Hin6I: # [GCGC] # 20 470 566 1211 2020 # # HindII: # [GTYRAC] # 5 369 478 632 696 2107 # # HindIII: # [AAGCTT] # 439 704 3144 # # HinfI: # [GANTC] # 28 44 66 73 203 205 # 236 248 293 399 420 460 # 1612 # # HpaI: # [GTTAAC] # 632 1174 2481 # # HpaII: # [CCGG] # 13 440 588 703 874 1669 # # HphI: # [GGTGA] # 27 156 211 215 277 457 # 600 961 1383 # # Hpy166II: # [GTNNAC] # 5 80 90 116 253 308 # 632 696 2107 # # Hpy178III*: # [TCNNGA] # 6 10 64 70 103 120 # 124 126 137 183 325 342 # 364 416 530 1367 # # Hpy188I: # [TCNGA] # 11 27 27 44 45 63 # 91 95 102 112 123 155 # 214 222 260 311 453 501 # 581 850 # # Hpy99I: # [CGWCG] # 12 111 354 566 666 1216 # 1362 # # KasI: # [GGCGCC] # 567 3720 # # KpnI: # [GGTACC] # 944 3343 # # Ksp632I*: # [CTCTTC] # 16 36 125 459 532 3119 # # MaeI: # [CTAG] # 65 127 159 215 369 374 # 417 507 631 661 762 # # MaeII: # [ACGT] # 94 246 309 507 951 2180 # # MaeIII: # [GTNAC] # 73 103 117 159 180 249 # 280 582 588 817 1139 # # MboI: # [GATC] # 6 10 15 32 46 99 # 104 107 148 231 252 389 # 712 975 1161 # # MboII: # [GAAGA] # 1 2 3 3 3 3 # 6 7 9 9 15 18 # 21 30 35 44 66 111 # 142 177 186 217 237 243 # 257 267 272 279 334 636 # 654 # # McrI*: # [CGRYCG] # 2071 2216 # # MfeI: # [CAATTG] # 164 4123 # # MlyI: # [GAGTC] # 1127 3160 # # MmeI: # [TCCRAC] # 159 880 1119 2129 # # MnlI: # [CCTC] # 15 33 36 37 48 50 # 62 80 100 107 142 177 # 183 231 303 396 432 531 # 598 726 # # MseI: # [TTAA] # 8 11 11 23 24 26 # 28 47 53 55 57 58 # 64 67 78 86 89 91 # 105 109 112 133 144 192 # 203 217 225 310 330 390 # 409 532 # # MslI: # [CAYNNNNRTG] # 2080 2207 # # MwoI: # [GCNNNNNNNGC] # 81 165 789 1300 1952 # # NarI: # [GGCGCC] # 566 3721 # # NcoI: # [CCATGG] # 932 1273 2082 # # NdeI: # [CATATG] # 304 951 3032 # # NheI: # [GCTAGC] # 2046 2241 # # NlaIII: # [CATG] # 13 16 121 147 263 405 # 621 623 2078 # # NlaIV: # [GGNNCC] # 1 109 132 179 192 238 # 456 553 572 728 1127 # # NspBII*: # [CMGCKG] # 111 290 1665 2221 # # NspI: # [RCATGY] # 268 4019 # # OliI: # [CACNNNNGTG] # 2080 2207 # # PflMI: # [CCANNNNNTGG] # 482 1085 1297 1423 # # PleI: # [GAGTC] # 1126 3161 # # PpuMI: # [RGGWCCY] # 1133 3154 # # PsiI: # [TTATAA] # 190 743 759 980 1615 # # PstI: # [CTGCAG] # 483 3804 # # PvuII: # [CAGCTG] # 111 290 3886 # # RsaI: # [GTAC] # 21 37 57 84 108 111 # 170 210 213 216 309 651 # 654 674 772 # # SanDI: # [GGGWCCC] # 1133 3154 # # SauI*: # [CCTNAGG] # 1441 2846 # # ScaI: # [AGTACT] # 229 654 890 2514 # # ScrFI: # [CCNGG] # 126 173 336 682 1467 1503 # # SecI*: # [CCNNGG] # 36 172 183 244 348 394 # 549 724 760 877 # # SetI: # [ASST] # 6 9 9 13 17 17 # 17 25 26 29 30 36 # 37 42 42 47 54 63 # 65 66 67 90 91 96 # 97 105 106 113 122 126 # 133 134 147 150 156 196 # 261 281 296 412 458 # # SexAI: # [ACCWGGT] # 1974 2313 # # SfaNI: # [GCATC] # 171 285 288 609 637 644 # 750 903 # # SfeI*: # [CTRYAG] # 198 281 3808 # # SmlI: # [CTYRAG] # 101 570 1133 2483 # # SpeI: # [ACTAGT] # 585 3702 # # SspI: # [AATATT] # 31 339 365 394 707 944 # 1507 # # StyI: # [CCWWGG] # 36 244 549 724 877 925 # 932 # # TaiI: # [ACGT] # 91 246 309 507 951 2183 # # TaqI: # [TCGA] # 13 47 58 60 67 219 # 234 238 522 657 1079 1093 # # TaqII: # [GACCGA] # 151 663 774 2699 # # TatI: # [WGTACW] # 216 229 656 674 1185 1327 # # TauI: # [GCSGC] # 214 600 1521 1952 # # TfiI: # [GAWTC] # 66 72 73 203 205 236 # 248 293 399 420 460 1612 # # TseI: # [GCWGC] # 3 6 108 119 470 1363 # 2218 # # TsoI: # [TARCCA] # 252 312 553 624 878 1668 # # Tsp45I: # [GTSAC] # 103 159 180 249 1170 2426 # # Tsp4CI*: # [ACNGT] # 9 35 49 65 67 86 # 143 177 224 372 388 427 # 1105 1140 # # TspDTI: # [ATGAA] # 6 9 34 35 38 46 # 47 55 57 57 66 66 # 80 82 101 121 143 143 # 145 181 233 247 291 309 # 401 571 723 # # TspEI: # [AATT] # 7 9 9 9 12 13 # 17 18 19 23 25 36 # 41 42 60 62 64 67 # 70 73 74 77 79 80 # 84 90 94 96 104 108 # 111 123 125 131 146 149 # 151 158 209 212 217 220 # 232 269 272 # # TspGWI: # [ACGGA] # 367 1332 2588 # # TspRI: # [CASTGNN] # 48 67 122 189 417 419 # 547 624 1854 # # TstI: # [CACNNNNNNTCC] # 32 32 579 1820 1824 # # VspI: # [ATTAAT] # 11 105 360 698 864 2249 # # XcmI: # [CCANNNNNNNNNTGG] # 463 1570 2254 # # XhoII: # [RGATCY] # 483 3804 # # XmnI: # [GAANNNNTTC] # 1876 2411 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4287 # Reported_sequences: 1 # Reported_hitcount: 930 #---------------------------------------