Restriction Fragments for END3/YNL084C



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The currently selected gene/sequence is :

END3/YNL084C on chromosome XIV from coordinates 471102 to 470053.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24197.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 20 + TspEI AATT 16 20 . . 35 39 + BsrI ACTGG 40 38 . . 44 48 - MboII GAAGA 36 35 . . 48 53 + BmeT110I CYCGRG 49 51 . . 48 53 + AvaI CYCGRG 48 52 . . 74 77 + SetI ASST 77 73 . . 76 81 + Hpy166II GTNNAC 78 78 . . 81 91 + BsiYI* CCNNNNNNNGG 87 84 . . 82 85 + FatI CATG 81 85 . . 82 85 + NlaIII CATG 85 81 . . 82 85 + CviAII CATG 82 84 . . 86 92 + SexAI ACCWGGT 86 91 . . 87 91 + BssKI CCNGG 86 91 . . 87 91 + BseBI CCWGG 88 89 . . 87 91 + ScrFI CCNGG 88 89 . . 87 91 + EcoRII CCWGG 86 91 . . 89 92 + SetI ASST 92 88 . . 99 102 + TspEI AATT 98 102 . . 105 110 + PsiI TTATAA 107 107 . . 109 112 + TspEI AATT 108 112 . . 116 119 + TspEI AATT 115 119 . . 121 125 + TfiI GAWTC 121 124 . . 121 125 + HinfI GANTC 121 124 . . 123 127 - TspDTI ATGAA 113 111 . . 153 156 + SetI ASST 156 152 . . 153 156 + CviJI RGCY 154 154 . . 153 156 + AluI AGCT 154 154 . . 168 172 + Hpy99I CGWCG 172 167 . . 175 178 + TspEI AATT 174 178 . . 187 190 - MnlI CCTC 180 179 . . 190 195 + ApoI RAATTY 190 194 . . 191 194 + TspEI AATT 190 194 . . 198 201 + TspEI AATT 197 201 . . 202 205 + CviRI* TGCA 203 203 . . 204 207 + FatI CATG 203 207 . . 204 207 + NlaIII CATG 207 203 . . 204 207 + CviAII CATG 204 206 . . 210 215 + VspI ATTAAT 211 213 . . 211 214 + MseI TTAA 211 213 . . 213 218 + SspI AATATT 215 215 . . 227 230 + MseI TTAA 227 229 . . 224 229 - TsoI TARCCA 214 212 . . 241 244 + SetI ASST 244 240 . . 241 244 + CviJI RGCY 242 242 . . 241 244 + AluI AGCT 242 242 . . 249 254 + Hpy178III* TCNNGA 250 252 . . 254 258 + TspDTI ATGAA 269 267 . . 257 260 + TspEI AATT 256 260 . . 273 277 + TfiI GAWTC 273 276 . . 273 277 + HinfI GANTC 273 276 . . 277 281 + BssKI CCNGG 276 281 . . 277 281 + BseBI CCWGG 278 279 . . 277 282 + SecI* CCNNGG 277 281 . . 277 281 + ScrFI CCNGG 278 279 . . 277 281 + EcoRII CCWGG 276 281 . . 287 290 + SetI ASST 290 286 . . 288 292 + HphI GGTGA 300 299 . . 290 301 + BdaI TGANNNNNNTCA 279 277 313 311 291 295 + TfiI GAWTC 291 294 . . 291 295 + HinfI GANTC 291 294 . . 290 301 - BdaI TGANNNNNNTCA 313 311 279 277 315 318 + Hin6I GCGC 315 317 . . 315 318 + GlaI GCGC 316 316 . . 315 318 + HhaI GCGC 317 315 . . 317 322 + Cac8I GCNNGC 319 319 . . 343 346 + MnlI CCTC 353 352 . . 351 355 - Hin4II* CCTTC 345 344 . . 372 375 + Csp6I GTAC 372 374 . . 372 375 + RsaI GTAC 373 373 . . 374 379 + BspLU11I* ACATGT 374 378 . . 374 379 + NspI RCATGY 378 374 . . 374 379 + AflIII ACRYGT 374 378 . . 375 378 + FatI CATG 374 378 . . 375 378 + NlaIII CATG 378 374 . . 375 378 + CviAII CATG 375 377 . . 378 382 + BsmAI GTCTC 383 387 . . 381 386 + Hpy178III* TCNNGA 382 384 . . 380 385 - Eco57MI CTGRAG 365 363 . . 380 385 - GsuI CTGGAG 365 363 . . 387 398 + BdaI TGANNNNNNTCA 376 374 410 408 388 397 + BsaBI GATNNNNATC 392 392 . . 393 397 + TfiI GAWTC 393 396 . . 393 397 + HinfI GANTC 393 396 . . 387 398 - BdaI TGANNNNNNTCA 410 408 376 374 401 405 + TspDTI ATGAA 416 414 . . 407 412 + ApoI RAATTY 407 411 . . 408 411 + TspEI AATT 407 411 . . 411 416 + PsiI TTATAA 413 413 . . 418 421 + SetI ASST 421 417 . . 418 421 + CviJI RGCY 419 419 . . 418 421 + AluI AGCT 419 419 . . 423 426 + CviRI* TGCA 424 424 . . 440 443 + Csp6I GTAC 440 442 . . 436 440 - BccI CCATC 430 431 . . 440 443 + RsaI GTAC 441 441 . . 459 463 + Tsp4CI* ACNGT 461 460 . . 462 467 + Hpy166II GTNNAC 464 464 . . 462 467 + HindII GTYRAC 464 464 . . 481 486 + ApoI RAATTY 481 485 . . 482 485 + TspEI AATT 481 485 . . 488 491 + MseI TTAA 488 490 . . 506 513 + SwaI ATTTAAAT 509 509 . . 507 512 + AhaIII* TTTAAA 509 509 . . 508 511 + MseI TTAA 508 510 . . 515 518 + SetI ASST 518 514 . . 526 533 + PacI TTAATTAA 530 528 . . 526 529 + MseI TTAA 526 528 . . 528 531 + TspEI AATT 527 531 . . 530 533 + MseI TTAA 530 532 . . 540 545 + ApoI RAATTY 540 544 . . 541 544 + TspEI AATT 540 544 . . 535 546 - CspCI CAANNNNNGTGG 559 557 524 522 547 551 + BccI CCATC 555 556 . . 551 556 + MfeI CAATTG 551 555 . . 552 555 + TspEI AATT 551 555 . . 556 565 + BsaBI GATNNNNATC 560 560 . . 561 566 + XhoII RGATCY 561 565 . . 562 565 + DpnI GATC 563 563 . . 562 565 + BstKTI GATC 564 562 . . 562 565 + MboI GATC 561 565 . . 562 566 - BinI* GGATC 556 557 . . 583 589 + TspRI CASTGNN 589 580 . . 584 588 + Tsp4CI* ACNGT 586 585 . . 589 592 + MseI TTAA 589 591 . . 608 613 - TaqII CACCCA 598 596 . . 617 622 + ApoI RAATTY 617 621 . . 618 621 + TspEI AATT 617 621 . . 629 634 + Hpy166II GTNNAC 631 631 . . 636 641 + ApoI RAATTY 636 640 . . 637 640 + TspEI AATT 636 640 . . 645 650 + EciI GGCGGA 661 659 . . 646 649 - AciI CCGC 646 648 . . 650 653 + SetI ASST 653 649 . . 649 652 - MnlI CCTC 642 641 . . 655 658 + CviRI* TGCA 656 656 . . 670 674 + DdeI CTNAG 670 673 . . 673 676 + SetI ASST 676 672 . . 673 676 + CviJI RGCY 674 674 . . 673 676 + AluI AGCT 674 674 . . 670 674 - BspCNI CTCAG 662 660 . . 670 674 - BseMII CTCAG 661 659 . . 679 689 + Hin4I GAYNNNNNVTC 670 665 702 697 684 688 + Tsp4CI* ACNGT 686 685 . . 686 690 + BmgT120I GGNCC 687 688 . . 686 690 + AvaII GGWCC 686 689 . . 686 690 + AsuI* GGNCC 686 689 . . 689 692 + MnlI CCTC 699 698 . . 679 689 - Hin4I GAYNNNNNVTC 702 697 670 665 696 699 + SetI ASST 699 695 . . 697 700 + MnlI CCTC 707 706 . . 715 719 + MboII GAAGA 727 726 . . 720 723 + SetI ASST 723 719 . . 720 723 + CviJI RGCY 721 721 . . 720 723 + AluI AGCT 721 721 . . 730 735 + Hpy166II GTNNAC 732 732 . . 743 747 + Tsp45I GTSAC 742 747 . . 743 747 + MaeIII GTNAC 742 747 . . 756 768 + AloI GAACNNNNNNTCC 748 743 780 775 758 765 + PspXI VCTCGAGB 759 763 . . 759 764 + SmlI CTYRAG 759 763 . . 759 764 + XhoI CTCGAG 759 763 . . 759 764 + BmeT110I CYCGRG 760 762 . . 759 764 + AvaI CYCGRG 759 763 . . 760 763 + TaqI TCGA 760 762 . . 766 770 + Hpy188I TCNGA 768 767 . . 773 778 + BtsI GCAGTG 780 778 . . 774 780 + TspRI CASTGNN 780 771 . . 779 790 + AlfI GCANNNNNNTGC 768 766 802 800 782 786 + MaeIII GTNAC 781 786 . . 785 788 + TaiI ACGT 788 784 . . 785 788 + SetI ASST 788 784 . . 785 788 + MaeII ACGT 785 787 . . 797 801 + TspDTI ATGAA 812 810 . . 799 803 + MboII GAAGA 811 810 . . 799 804 + BbvII* GAAGAC 806 810 . . 803 807 + Tsp4CI* ACNGT 805 804 . . 813 824 + AlfI GCANNNNNNTGC 802 800 836 834 813 824 - AlfI GCANNNNNNTGC 836 834 802 800 828 832 + Hpy188I TCNGA 830 829 . . 831 841 + Hin4I GAYNNNNNVTC 822 817 854 849 837 842 + BclI TGATCA 837 841 . . 838 841 + DpnI GATC 839 839 . . 838 841 + BstKTI GATC 840 838 . . 838 841 + MboI GATC 837 841 . . 831 841 - Hin4I GAYNNNNNVTC 854 849 822 817 852 855 + FatI CATG 851 855 . . 852 855 + NlaIII CATG 855 851 . . 852 855 + CviAII CATG 852 854 . . 863 866 + TspEI AATT 862 866 . . 870 875 + StuI AGGCCT 872 872 . . 871 874 + CviJI RGCY 872 872 . . 871 874 + HaeIII GGCC 872 872 . . 868 872 - Hin4II* CCTTC 862 861 . . 894 905 + BdaI TGANNNNNNTCA 883 881 917 915 902 907 + SmlI CTYRAG 902 906 . . 894 905 - BdaI TGANNNNNNTCA 917 915 883 881 902 907 - Bce83I* CTTGAG 887 885 . . 910 913 + CviJI RGCY 911 911 . . 908 911 - MnlI CCTC 901 900 . . 923 927 + DdeI CTNAG 923 926 . . 930 939 + BsaBI GATNNNNATC 934 934 . . 927 930 - AciI CCGC 927 929 . . 932 935 + MseI TTAA 932 934 . . 940 943 + SetI ASST 943 939 . . 943 946 + TaqI TCGA 943 945 . . 973 978 + EcoP15I CAGCAG 1003 1005 . . 977 980 + SetI ASST 980 976 . . 975 980 - BspMI ACCTGC 966 970 . . 994 997 + TspEI AATT 993 997 . . 1011 1015 - BsmAI GTCTC 1005 1009 . . 1019 1022 + TspEI AATT 1018 1022 . . 1022 1025 + CviRI* TGCA 1023 1023 . . 1023 1028 + Cac8I GCNNGC 1025 1025 . . 1027 1037 + MwoI GCNNNNNNNGC 1033 1030 . . 1044 1049 + MfeI CAATTG 1044 1048 . . 1045 1048 + TspEI AATT 1044 1048 . . #--------------------------------------- # # # AciI: # [CCGC] # 123 281 646 # # AflIII: # [ACRYGT] # 374 676 # # AhaIII*: # [TTTAAA] # 509 541 # # AlfI: # [GCANNNNNNTGC] # 0 0 0 34 34 214 # 768 # # AloI: # [GAACNNNNNNTCC] # 32 270 748 # # AluI: # [AGCT] # 47 88 154 177 255 329 # # ApoI: # [RAATTY] # 19 59 74 77 190 217 # 414 # # AsuI*: # [GGNCC] # 364 686 # # AvaI: # [CYCGRG] # 48 291 711 # # AvaII: # [GGWCC] # 364 686 # # BbvII*: # [GAAGAC] # 244 806 # # BccI: # [CCATC] # 125 430 495 # # Bce83I*: # [CTTGAG] # 163 887 # # BclI: # [TGATCA] # 213 837 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 34 34 34 63 133 279 # 473 # # BinI*: # [GGATC] # 494 556 # # BmeT110I: # [CYCGRG] # 49 290 711 # # BmgT120I: # [GGNCC] # 363 687 # # BsaBI: # [GATNNNNATC] # 116 168 374 392 # # BseBI: # [CCWGG] # 88 190 772 # # BseMII: # [CTCAG] # 389 661 # # BsiYI*: # [CCNNNNNNNGG] # 87 963 # # BsmAI: # [GTCTC] # 45 383 622 # # BspCNI: # [CTCAG] # 388 662 # # BspLU11I*: # [ACATGT] # 374 676 # # BspMI: # [ACCTGC] # 84 966 # # BsrI: # [ACTGG] # 40 1010 # # BssKI: # [CCNGG] # 86 190 774 # # BstKTI: # [GATC] # 210 276 564 # # BtsI: # [GCAGTG] # 270 780 # # Cac8I: # [GCNNGC] # 25 319 706 # # Csp6I: # [GTAC] # 68 372 610 # # CspCI: # [CAANNNNNGTGG] # 35 491 524 # # CviAII: # [CATG] # 82 122 171 198 477 # # CviJI: # [RGCY] # 39 47 88 139 151 154 # 177 255 # # CviRI*: # [TGCA] # 27 203 221 232 367 # # DdeI: # [CTNAG] # 127 253 670 # # DpnI: # [GATC] # 211 276 563 # # EciI: # [GGCGGA] # 389 661 # # Eco57MI: # [CTGRAG] # 365 685 # # EcoP15I: # [CAGCAG] # 47 1003 # # EcoRII: # [CCWGG] # 86 190 774 # # FatI: # [CATG] # 81 122 171 199 477 # # GlaI: # [GCGC] # 316 734 # # GsuI: # [CTGGAG] # 365 685 # # HaeIII: # [GGCC] # 178 872 # # HhaI: # [GCGC] # 317 733 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 120 196 670 # # Hin4II*: # [CCTTC] # 188 345 517 # # Hin6I: # [GCGC] # 315 735 # # HindII: # [GTYRAC] # 464 586 # # HinfI: # [GANTC] # 18 102 121 152 657 # # HphI: # [GGTGA] # 300 750 # # Hpy166II: # [GTNNAC] # 78 101 167 318 386 # # Hpy178III*: # [TCNNGA] # 132 250 668 # # Hpy188I: # [TCNGA] # 62 220 768 # # Hpy99I: # [CGWCG] # 172 878 # # MaeII: # [ACGT] # 265 785 # # MaeIII: # [GTNAC] # 39 269 742 # # MboI: # [GATC] # 213 276 561 # # MboII: # [GAAGA] # 36 84 239 691 # # MfeI: # [CAATTG] # 6 493 551 # # MnlI: # [CCTC] # 8 57 149 173 180 194 # 289 # # MseI: # [TTAA] # 4 16 18 20 59 118 # 211 261 343 # # MwoI: # [GCNNNNNNNGC] # 17 1033 # # NlaIII: # [CATG] # 85 122 171 195 477 # # NspI: # [RCATGY] # 378 672 # # PacI: # [TTAATTAA] # 520 530 # # PsiI: # [TTATAA] # 107 306 637 # # PspXI: # [VCTCGAGB] # 291 759 # # RsaI: # [GTAC] # 68 373 609 # # ScrFI: # [CCNGG] # 88 190 772 # # SecI*: # [CCNNGG] # 277 773 # # SetI: # [ASST] # 15 23 23 24 37 46 # 64 65 70 77 88 97 # 131 135 155 # # SexAI: # [ACCWGGT] # 86 964 # # SmlI: # [CTYRAG] # 143 148 759 # # SspI: # [AATATT] # 215 835 # # StuI: # [AGGCCT] # 178 872 # # SwaI: # [ATTTAAAT] # 509 541 # # TaiI: # [ACGT] # 262 788 # # TaqI: # [TCGA] # 107 183 760 # # TaqII: # [CACCCA] # 452 598 # # TfiI: # [GAWTC] # 18 102 121 152 657 # # TsoI: # [TARCCA] # 214 836 # # Tsp45I: # [GTSAC] # 308 742 # # Tsp4CI*: # [ACNGT] # 100 119 125 245 461 # # TspDTI: # [ATGAA] # 113 147 156 238 396 # # TspEI: # [AATT] # 6 7 7 10 11 13 # 16 16 19 25 26 46 # 59 59 66 74 82 131 # 151 226 # # TspRI: # [CASTGNN] # 191 270 589 # # VspI: # [ATTAAT] # 211 839 # # XhoI: # [CTCGAG] # 291 759 # # XhoII: # [RGATCY] # 489 561 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1050 # Reported_sequences: 1 # Reported_hitcount: 216 #---------------------------------------