Restriction Fragments for KSH1/YNL024C-A



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The currently selected gene/sequence is :

KSH1/YNL024C-A on chromosome XIV from coordinates 586820 to 586602.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.31760.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 9 + CviRI* TGCA 7 7 . . 14 17 + MseI TTAA 14 16 . . 16 19 + TspEI AATT 15 19 . . 26 31 + Ksp632I* CTCTTC 32 35 . . 27 31 - MboII GAAGA 19 18 . . 32 35 + CviRI* TGCA 33 33 . . 45 49 + MaeIII GTNAC 44 49 . . 59 62 + FatI CATG 58 62 . . 59 62 + NlaIII CATG 62 58 . . 59 62 + CviAII CATG 59 61 . . 57 61 - TspDTI ATGAA 47 45 . . 73 76 + FatI CATG 72 76 . . 73 76 + NlaIII CATG 76 72 . . 73 76 + CviAII CATG 73 75 . . 71 75 - TspDTI ATGAA 61 59 . . 78 83 + BtsI GCAGTG 85 83 . . 79 85 + TspRI CASTGNN 85 76 . . 82 87 + CfrI YGGCCR 82 86 . . 83 86 + CviJI RGCY 84 84 . . 83 86 + HaeIII GGCC 84 84 . . 86 91 + Esp3I CGTCTC 92 96 . . 87 91 + BsmAI GTCTC 92 96 . . 84 88 - BceAI ACGGC 69 71 . . 94 97 + MaeI CTAG 94 96 . . 97 100 + DpnI GATC 98 98 . . 97 100 + BstKTI GATC 99 97 . . 97 100 + MboI GATC 96 100 . . 101 105 + MaeIII GTNAC 100 105 . . 111 116 + Hpy178III* TCNNGA 112 114 . . 111 116 + BspHI TCATGA 111 115 . . 112 115 + FatI CATG 111 115 . . 112 115 + NlaIII CATG 115 111 . . 112 115 + CviAII CATG 112 114 . . 116 119 + SetI ASST 119 115 . . 115 118 - MnlI CCTC 108 107 . . 124 130 + DraII RGGNCCY 125 128 . . 124 129 + NlaIV GGNNCC 126 126 . . 125 129 + BmgT120I GGNCC 126 127 . . 125 129 + AsuI* GGNCC 125 128 . . 126 129 + CviJI RGCY 127 127 . . 126 129 + HaeIII GGCC 127 127 . . 128 132 + Hin4II* CCTTC 138 137 . . 131 136 + Hpy178III* TCNNGA 132 134 . . 143 148 + BsaAI YACGTR 145 145 . . 143 148 + PmaCI CACGTG 145 145 . . 144 147 + TaiI ACGT 147 143 . . 144 147 + SetI ASST 147 143 . . 144 147 + MaeII ACGT 144 146 . . 144 149 + AflIII ACRYGT 144 148 . . 151 155 + HphI GGTGA 163 162 . . 158 162 + BmgT120I GGNCC 159 160 . . 158 162 + AsuI* GGNCC 158 161 . . 159 162 + CviJI RGCY 160 160 . . 159 162 + HaeIII GGCC 160 160 . . 161 165 - BsrI ACTGG 161 159 . . 167 172 + NdeI CATATG 168 170 . . 181 186 + NspI RCATGY 185 181 . . 182 185 + FatI CATG 181 185 . . 182 185 + NlaIII CATG 185 181 . . 182 185 + CviAII CATG 182 184 . . 191 202 + BdaI TGANNNNNNTCA 180 178 214 212 192 201 + BsaBI GATNNNNATC 196 196 . . 194 199 + BalI TGGCCA 196 196 . . 194 199 + CfrI YGGCCR 194 198 . . 195 198 + CviJI RGCY 196 196 . . 195 198 + HaeIII GGCC 196 196 . . 192 196 - BccI CCATC 186 187 . . 197 201 + BccI CCATC 205 206 . . 191 202 - BdaI TGANNNNNNTCA 214 212 180 178 206 209 + TspEI AATT 205 209 . . 209 212 + MseI TTAA 209 211 . . 213 218 + PvuII CAGCTG 215 215 . . 213 218 + NspBII* CMGCKG 215 215 . . 214 217 + SetI ASST 217 213 . . 214 217 + CviJI RGCY 215 215 . . 214 217 + AluI AGCT 215 215 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 75 144 # # AluI: # [AGCT] # 4 215 # # AsuI*: # [GGNCC] # 33 61 125 # # BalI: # [TGGCCA] # 23 196 # # BccI: # [CCATC] # 14 19 186 # # BceAI: # [ACGGC] # 69 150 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 180 # # BmgT120I: # [GGNCC] # 33 60 126 # # BsaAI: # [YACGTR] # 74 145 # # BsaBI: # [GATNNNNATC] # 23 196 # # BsmAI: # [GTCTC] # 92 127 # # BspHI: # [TCATGA] # 108 111 # # BsrI: # [ACTGG] # 58 161 # # BstKTI: # [GATC] # 99 120 # # BtsI: # [GCAGTG] # 85 134 # # CfrI: # [YGGCCR] # 25 82 112 # # CviAII: # [CATG] # 14 37 39 59 70 # # CviJI: # [RGCY] # 4 19 33 36 43 84 # # CviRI*: # [TGCA] # 7 26 186 # # DpnI: # [GATC] # 98 121 # # DraII: # [RGGNCCY] # 94 125 # # Esp3I: # [CGTCTC] # 92 127 # # FatI: # [CATG] # 14 38 39 58 70 # # HaeIII: # [GGCC] # 23 33 36 43 84 # # Hin4II*: # [CCTTC] # 81 138 # # HphI: # [GGTGA] # 56 163 # # Hpy178III*: # [TCNNGA] # 20 87 112 # # Ksp632I*: # [CTCTTC] # 32 187 # # MaeI: # [CTAG] # 94 125 # # MaeII: # [ACGT] # 75 144 # # MaeIII: # [GTNAC] # 44 56 119 # # MboI: # [GATC] # 96 123 # # MboII: # [GAAGA] # 19 200 # # MnlI: # [CCTC] # 108 111 # # MseI: # [TTAA] # 10 14 195 # # NdeI: # [CATATG] # 51 168 # # NlaIII: # [CATG] # 14 34 39 62 70 # # NlaIV: # [GGNNCC] # 93 126 # # NspBII*: # [CMGCKG] # 4 215 # # NspI: # [RCATGY] # 34 185 # # PmaCI: # [CACGTG] # 74 145 # # PvuII: # [CAGCTG] # 4 215 # # SetI: # [ASST] # 2 28 70 119 # # TaiI: # [ACGT] # 72 147 # # TspDTI: # [ATGAA] # 14 47 158 # # TspEI: # [AATT] # 14 15 190 # # TspRI: # [CASTGNN] # 85 134 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 219 # Reported_sequences: 1 # Reported_hitcount: 76 #---------------------------------------