Restriction Fragments for YMRWdelta21



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YMRWdelta21 on chromosome XIII from coordinates 837450 to 837764.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28407.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + TspEI AATT 2 6 . . 5 8 + MseI TTAA 5 7 . . 14 19 + VspI ATTAAT 15 17 . . 15 18 + MseI TTAA 15 17 . . 27 32 + SspI AATATT 29 29 . . 33 37 + BsrI ACTGG 38 36 . . 42 47 + PsiI TTATAA 44 44 . . 51 54 - AciI CCGC 51 53 . . 104 107 + SetI ASST 107 103 . . 104 107 + CviJI RGCY 105 105 . . 104 107 + AluI AGCT 105 105 . . 106 111 + Eco57MI CTGRAG 127 125 . . 106 111 + Eco57I CTGAAG 127 125 . . 112 115 + CviRI* TGCA 113 113 . . 139 143 + TspDTI ATGAA 154 152 . . 152 156 + TspGWI ACGGA 167 165 . . 159 162 - MnlI CCTC 152 151 . . 159 169 - Hin4I GAYNNNNNVTC 182 177 150 145 172 175 + TspEI AATT 171 175 . . 194 198 + TfiI GAWTC 194 197 . . 194 198 + HinfI GANTC 194 197 . . 207 216 + BsaBI GATNNNNATC 211 211 . . 216 223 + PspXI VCTCGAGB 217 221 . . 216 219 + MnlI CCTC 226 225 . . 216 223 + AbsI CCTCGAGG 217 221 . . 217 222 + SmlI CTYRAG 217 221 . . 217 222 + XhoI CTCGAG 217 221 . . 217 222 + BmeT110I CYCGRG 218 220 . . 217 222 + AvaI CYCGRG 217 221 . . 218 221 + TaqI TCGA 218 220 . . 220 225 + BseRI GAGGAG 235 233 . . 220 223 - MnlI CCTC 213 212 . . 243 248 + Hpy166II GTNNAC 245 245 . . 244 249 + TatI WGTACW 244 248 . . 244 249 + Bsp1407I TGTACA 244 248 . . 245 248 + Csp6I GTAC 245 247 . . 245 248 + RsaI GTAC 246 246 . . 251 256 + SspI AATATT 253 253 . . 261 264 + CviJI RGCY 262 262 . . 266 271 + BclI TGATCA 266 270 . . 267 270 + DpnI GATC 268 268 . . 267 270 + BstKTI GATC 269 267 . . 267 270 + MboI GATC 266 270 . . 276 279 + CviRI* TGCA 277 277 . . 288 291 + TspEI AATT 287 291 . . 296 299 + TspEI AATT 295 299 . . 302 306 - HphI GGTGA 294 293 . . 308 313 + SspI AATATT 310 310 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 98 217 # # AciI: # [CCGC] # 51 264 # # AluI: # [AGCT] # 105 210 # # AvaI: # [CYCGRG] # 98 217 # # BclI: # [TGATCA] # 49 266 # # BmeT110I: # [CYCGRG] # 97 218 # # BsaBI: # [GATNNNNATC] # 104 211 # # BseRI: # [GAGGAG] # 80 235 # # Bsp1407I: # [TGTACA] # 71 244 # # BsrI: # [ACTGG] # 38 277 # # BstKTI: # [GATC] # 46 269 # # Csp6I: # [GTAC] # 70 245 # # CviJI: # [RGCY] # 53 105 157 # # CviRI*: # [TGCA] # 38 113 164 # # DpnI: # [GATC] # 47 268 # # Eco57I: # [CTGAAG] # 127 188 # # Eco57MI: # [CTGRAG] # 127 188 # # Hin4I: # [GAYNNNNNVTC] # 32 133 150 # # HinfI: # [GANTC] # 121 194 # # HphI: # [GGTGA] # 21 294 # # Hpy166II: # [GTNNAC] # 70 245 # # MboI: # [GATC] # 49 266 # # MnlI: # [CCTC] # 13 61 89 152 # # MseI: # [TTAA] # 5 10 300 # # PsiI: # [TTATAA] # 44 271 # # PspXI: # [VCTCGAGB] # 98 217 # # RsaI: # [GTAC] # 69 246 # # SetI: # [ASST] # 107 208 # # SmlI: # [CTYRAG] # 98 217 # # SspI: # [AATATT] # 5 29 57 224 # # TaqI: # [TCGA] # 97 218 # # TatI: # [WGTACW] # 71 244 # # TfiI: # [GAWTC] # 121 194 # # TspDTI: # [ATGAA] # 154 161 # # TspEI: # [AATT] # 2 8 20 116 169 # # TspGWI: # [ACGGA] # 148 167 # # VspI: # [ATTAAT] # 15 300 # # XhoI: # [CTCGAG] # 98 217 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 315 # Reported_sequences: 1 # Reported_hitcount: 48 #---------------------------------------