Restriction Fragments for YMRWdelta20



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YMRWdelta20 on chromosome XIII from coordinates 809000 to 809233.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5713.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + DdeI CTNAG 4 7 . . 17 22 + Hpy166II GTNNAC 19 19 . . 17 22 + BssNAI GTATAC 19 19 . . 17 22 + AccI GTMKAC 18 20 . . 21 25 + Tsp4CI* ACNGT 23 22 . . 31 35 + FokI GGATG 44 48 . . 31 35 + BseGI GGATG 37 35 . . 29 32 - MnlI CCTC 22 21 . . 40 43 + DpnI GATC 41 41 . . 40 43 + BstKTI GATC 42 40 . . 40 43 + MboI GATC 39 43 . . 47 51 + BmgT120I GGNCC 48 49 . . 47 51 + AvaII GGWCC 47 50 . . 47 51 + AsuI* GGNCC 47 50 . . 72 77 + NlaIV GGNNCC 74 74 . . 72 77 + HgiCI* GGYRCC 72 76 . . 76 87 + BstXI CCANNNNNNTGG 83 79 . . 76 80 - BsrI ACTGG 76 74 . . 81 84 + MseI TTAA 81 83 . . 86 91 + NlaIV GGNNCC 88 88 . . 89 92 + SetI ASST 92 88 . . 90 93 + MnlI CCTC 100 99 . . 92 95 + TaqI TCGA 92 94 . . 94 99 + BsmI GAATGC 100 98 . . 97 100 + CviRI* TGCA 98 98 . . 100 103 + TspEI AATT 99 103 . . 111 115 + TspGWI ACGGA 126 124 . . 121 124 + MseI TTAA 121 123 . . 134 137 + SetI ASST 137 133 . . 141 144 + SetI ASST 144 140 . . 140 143 - MnlI CCTC 133 132 . . 143 146 + Csp6I GTAC 143 145 . . 143 146 + RsaI GTAC 144 144 . . 161 166 + ApoI RAATTY 161 165 . . 162 165 + TspEI AATT 161 165 . . 166 170 + Hpy188I TCNGA 168 167 . . 183 187 - TspDTI ATGAA 173 171 . . 192 197 + Hpy178III* TCNNGA 193 195 . . 193 198 + SmlI CTYRAG 193 197 . . 193 198 + XhoI CTCGAG 193 197 . . 193 198 + BmeT110I CYCGRG 194 196 . . 193 198 + AvaI CYCGRG 193 197 . . 194 197 + TaqI TCGA 194 196 . . 194 199 + Hpy178III* TCNNGA 195 197 . . 198 202 + MboII GAAGA 210 209 . . 206 210 + DdeI CTNAG 206 209 . . 226 231 + SspI AATATT 228 228 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 18 216 # # ApoI: # [RAATTY] # 73 161 # # AsuI*: # [GGNCC] # 47 187 # # AvaI: # [CYCGRG] # 41 193 # # AvaII: # [GGWCC] # 47 187 # # BmeT110I: # [CYCGRG] # 40 194 # # BmgT120I: # [GGNCC] # 48 186 # # BseGI: # [GGATG] # 37 197 # # BsmI: # [GAATGC] # 100 134 # # BsrI: # [ACTGG] # 76 158 # # BssNAI: # [GTATAC] # 19 215 # # BstKTI: # [GATC] # 42 192 # # BstXI: # [CCANNNNNNTGG] # 83 151 # # Csp6I: # [GTAC] # 91 143 # # CviRI*: # [TGCA] # 98 136 # # DdeI: # [CTNAG] # 4 28 202 # # DpnI: # [GATC] # 41 193 # # FokI: # [GGATG] # 44 190 # # HgiCI*: # [GGYRCC] # 72 162 # # Hpy166II: # [GTNNAC] # 19 215 # # Hpy178III*: # [TCNNGA] # 2 39 193 # # Hpy188I: # [TCNGA] # 66 168 # # MboI: # [GATC] # 39 195 # # MboII: # [GAAGA] # 24 210 # # MnlI: # [CCTC] # 22 33 78 101 # # MseI: # [TTAA] # 40 81 113 # # NlaIV: # [GGNNCC] # 14 74 146 # # RsaI: # [GTAC] # 90 144 # # SetI: # [ASST] # 7 45 90 92 # # SmlI: # [CTYRAG] # 41 193 # # SspI: # [AATATT] # 6 228 # # TaqI: # [TCGA] # 40 92 102 # # Tsp4CI*: # [ACNGT] # 23 211 # # TspDTI: # [ATGAA] # 61 173 # # TspEI: # [AATT] # 62 73 99 # # TspGWI: # [ACGGA] # 108 126 # # XhoI: # [CTCGAG] # 41 193 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 234 # Reported_sequences: 1 # Reported_hitcount: 47 #---------------------------------------