Restriction Fragments for YMRCdelta8



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The currently selected gene/sequence is :

YMRCdelta8 on chromosome XIII from coordinates 362918 to 362587.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22773.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 65 69 + Tsp4CI* ACNGT 67 66 . . 71 74 + MseI TTAA 71 73 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 93 103 - Hin4I GAYNNNNNVTC 116 111 84 79 105 108 + TaqI TCGA 105 107 . . 114 117 - MnlI CCTC 107 106 . . 119 122 + SetI ASST 122 118 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + AluI AGCT 120 120 . . 121 126 + Eco57MI CTGRAG 142 140 . . 121 126 + Eco57I CTGAAG 142 140 . . 127 130 + CviRI* TGCA 128 128 . . 154 158 + TspDTI ATGAA 169 167 . . 168 172 + TspGWI ACGGA 183 181 . . 175 178 - MnlI CCTC 168 167 . . 175 185 - Hin4I GAYNNNNNVTC 198 193 166 161 188 193 + SspI AATATT 190 190 . . 210 214 + TfiI GAWTC 210 213 . . 210 214 + HinfI GANTC 210 213 . . 214 224 + BsiYI* CCNNNNNNNGG 220 217 . . 224 228 + TfiI GAWTC 224 227 . . 221 224 - MnlI CCTC 214 213 . . 224 228 + HinfI GANTC 224 227 . . 236 241 + SmlI CTYRAG 236 240 . . 236 241 + Bce83I* CTTGAG 257 255 . . 239 244 + BseRI GAGGAG 254 252 . . 239 242 - MnlI CCTC 232 231 . . 250 253 + MaeI CTAG 250 252 . . 262 267 + Hpy166II GTNNAC 264 264 . . 262 267 + BssNAI GTATAC 264 264 . . 262 267 + AccI GTMKAC 263 265 . . 266 269 + SetI ASST 269 265 . . 270 275 + SspI AATATT 272 272 . . 278 281 + CviJI RGCY 279 279 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 69 263 # # AluI: # [AGCT] # 22 98 212 # # Bce83I*: # [CTTGAG] # 75 257 # # BseRI: # [GAGGAG] # 78 254 # # BsiYI*: # [CCNNNNNNNGG] # 112 220 # # BssNAI: # [GTATAC] # 68 264 # # CviJI: # [RGCY] # 22 53 98 159 # # CviRI*: # [TGCA] # 128 204 # # Eco57I: # [CTGAAG] # 142 190 # # Eco57MI: # [CTGRAG] # 142 190 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 84 134 # # HinfI: # [GANTC] # 14 37 71 210 # # Hpy166II: # [GTNNAC] # 68 264 # # MaeI: # [CTAG] # 82 250 # # MnlI: # [CCTC] # 18 46 61 100 107 # # MseI: # [TTAA] # 71 261 # # SetI: # [ASST] # 22 63 100 147 # # SmlI: # [CTYRAG] # 96 236 # # SspI: # [AATATT] # 60 82 190 # # TaqI: # [TCGA] # 105 227 # # TfiI: # [GAWTC] # 14 37 71 210 # # Tsp4CI*: # [ACNGT] # 67 265 # # TspDTI: # [ATGAA] # 163 169 # # TspEI: # [AATT] # 28 304 # # TspGWI: # [ACGGA] # 149 183 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 332 # Reported_sequences: 1 # Reported_hitcount: 39 #---------------------------------------