Restriction Fragments for YMRCdelta18



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The currently selected gene/sequence is :

YMRCdelta18 on chromosome XIII from coordinates 573338 to 573112.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2664.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 12 + TspDTI ATGAA 23 21 . . 14 18 + TspDTI ATGAA 29 27 . . 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 39 43 + MaeIII GTNAC 38 43 . . 48 52 - BsrI ACTGG 48 46 . . 60 63 + FatI CATG 59 63 . . 60 63 + NlaIII CATG 63 59 . . 60 63 + CviAII CATG 60 62 . . 63 66 + Csp6I GTAC 63 65 . . 63 66 + RsaI GTAC 64 64 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 86 89 + SetI ASST 89 85 . . 97 101 + Tsp4CI* ACNGT 99 98 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 102 105 + FatI CATG 101 105 . . 102 105 + NlaIII CATG 105 101 . . 102 105 + CviAII CATG 102 104 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 126 129 + CviRI* TGCA 127 127 . . 174 178 + MboII GAAGA 186 185 . . 187 192 + TatI WGTACW 187 191 . . 187 192 + ScaI AGTACT 189 189 . . 188 191 + Csp6I GTAC 188 190 . . 188 191 + RsaI GTAC 189 189 . . 209 212 + TspEI AATT 208 212 . . 213 223 + BsiYI* CCNNNNNNNGG 219 216 . . 223 227 + TfiI GAWTC 223 226 . . 220 223 - MnlI CCTC 213 212 . . 223 227 + HinfI GANTC 223 226 . . #--------------------------------------- # # # AluI: # [AGCT] # 108 119 # # BsiYI*: # [CCNNNNNNNGG] # 8 219 # # BsrI: # [ACTGG] # 48 179 # # Csp6I: # [GTAC] # 39 63 125 # # CviAII: # [CATG] # 42 60 125 # # CviJI: # [RGCY] # 108 119 # # CviRI*: # [TGCA] # 100 127 # # FatI: # [CATG] # 42 59 126 # # Hin4I: # [GAYNNNNNVTC] # 32 83 112 # # HinfI: # [GANTC] # 4 223 # # MaeI: # [CTAG] # 33 194 # # MaeIII: # [GTNAC] # 38 189 # # MboII: # [GAAGA] # 41 186 # # MnlI: # [CCTC] # 14 213 # # NlaIII: # [CATG] # 42 63 122 # # RsaI: # [GTAC] # 38 64 125 # # ScaI: # [AGTACT] # 38 189 # # SetI: # [ASST] # 32 89 106 # # SpeI: # [ACTAGT] # 32 195 # # TatI: # [WGTACW] # 40 187 # # TfiI: # [GAWTC] # 4 223 # # Tsp4CI*: # [ACNGT] # 32 67 128 # # TspDTI: # [ATGAA] # 6 23 198 # # TspEI: # [AATT] # 19 208 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 227 # Reported_sequences: 1 # Reported_hitcount: 32 #---------------------------------------