Restriction Fragments for ADE4/YMR300C



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The currently selected gene/sequence is :

ADE4/YMR300C on chromosome XIII from coordinates 867091 to 865559.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.4707.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 18 + SetI ASST 18 14 . . 41 46 - Eco57MI CTGRAG 26 24 . . 41 46 - GsuI CTGGAG 26 24 . . 43 47 - BsrI ACTGG 43 41 . . 48 51 + SetI ASST 51 47 . . 48 51 + CviJI RGCY 49 49 . . 48 51 + AluI AGCT 49 49 . . 51 56 + Hpy178III* TCNNGA 52 54 . . 51 56 + BetI* WCCGGW 51 55 . . 51 56 + BspMII* TCCGGA 51 55 . . 52 55 + HpaII CCGG 52 54 . . 67 71 + FokI GGATG 80 84 . . 67 71 + BseGI GGATG 73 71 . . 64 68 - BccI CCATC 58 59 . . 69 74 + EcoT22I ATGCAT 73 69 . . 70 73 + CviRI* TGCA 71 71 . . 68 72 - SfaNI GCATC 58 62 . . 89 94 + Hpy166II GTNNAC 91 91 . . 99 102 + CviRI* TGCA 100 100 . . 100 104 + BlsI GCNGC 102 101 . . 100 104 + BisI GCNGC 101 102 . . 100 104 + BbvI GCAGC 112 116 . . 100 104 + TseI GCWGC 100 103 . . 101 106 + PvuII CAGCTG 103 103 . . 101 106 + NspBII* CMGCKG 103 103 . . 97 101 - SfaNI GCATC 87 91 . . 102 105 + SetI ASST 105 101 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + AluI AGCT 103 103 . . 103 113 + MwoI GCNNNNNNNGC 109 106 . . 111 114 + SetI ASST 114 110 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + AluI AGCT 112 112 . . 115 118 + SetI ASST 118 114 . . 125 130 - BsiI* CACGAG 125 129 . . 134 138 + TfiI GAWTC 134 137 . . 134 138 + HinfI GANTC 134 137 . . 150 153 + SetI ASST 153 149 . . 162 165 + CviJI RGCY 163 163 . . 165 170 + Hpy178III* TCNNGA 166 168 . . 167 171 + Tsp45I GTSAC 166 171 . . 167 171 + MaeIII GTNAC 166 171 . . 164 169 - BsiI* CACGAG 164 168 . . 170 173 + TaiI ACGT 173 169 . . 170 173 + SetI ASST 173 169 . . 170 173 + MaeII ACGT 170 172 . . 186 189 + TaiI ACGT 189 185 . . 186 189 + SetI ASST 189 185 . . 186 189 + MaeII ACGT 186 188 . . 186 191 + AflIII ACRYGT 186 190 . . 193 198 + BetI* WCCGGW 193 197 . . 194 197 + HpaII CCGG 194 196 . . 205 210 + NlaIV GGNNCC 207 207 . . 209 214 + DsaI* CCRYGG 209 213 . . 209 214 + StyI CCWWGG 209 213 . . 209 214 + NcoI CCATGG 209 213 . . 209 214 + SecI* CCNNGG 209 213 . . 210 213 + FatI CATG 209 213 . . 210 213 + NlaIII CATG 213 209 . . 210 213 + CviAII CATG 210 212 . . 225 230 + SmlI CTYRAG 225 229 . . 225 230 + Bce83I* CTTGAG 246 244 . . 230 235 + EcoRV GATATC 232 232 . . 235 245 + BsiYI* CCNNNNNNNGG 241 238 . . 236 241 + SfeI* CTRYAG 236 240 . . 240 243 + CviJI RGCY 241 241 . . 242 246 + BssKI CCNGG 241 246 . . 242 245 + HpaII CCGG 242 244 . . 242 246 + ScrFI CCNGG 243 244 . . 242 246 + CauII* CCSGG 243 244 . . 247 251 - MboII GAAGA 239 238 . . 246 251 - BbvII* GAAGAC 239 243 . . 252 255 + CviJI RGCY 253 253 . . 256 259 + TspEI AATT 255 259 . . 259 263 + Hpy188I TCNGA 261 260 . . 265 268 + Hin6I GCGC 265 267 . . 265 268 + GlaI GCGC 266 266 . . 265 268 + HhaI GCGC 267 265 . . 278 281 + TaiI ACGT 281 277 . . 278 281 + SetI ASST 281 277 . . 278 281 + MaeII ACGT 278 280 . . 293 297 + Tsp4CI* ACNGT 295 294 . . 299 302 + MseI TTAA 299 301 . . 314 318 + Tsp4CI* ACNGT 316 315 . . 317 321 + MaeIII GTNAC 316 321 . . 324 328 + HphI GGTGA 336 335 . . 333 336 + CviRI* TGCA 334 334 . . 334 338 + SfaNI GCATC 343 347 . . 342 346 + MboII GAAGA 354 353 . . 342 347 - Ksp632I* CTCTTC 337 340 . . 354 358 + FokI GGATG 367 371 . . 354 358 + BseGI GGATG 360 358 . . 356 360 + TspDTI ATGAA 371 369 . . 357 368 + BdaI TGANNNNNNTCA 346 344 380 378 358 362 + MboII GAAGA 370 369 . . 358 363 + BbvII* GAAGAC 365 369 . . 358 367 + XmnI GAANNNNTTC 362 362 . . 362 365 + TaiI ACGT 365 361 . . 362 365 + SetI ASST 365 361 . . 362 365 + MaeII ACGT 362 364 . . 357 368 - BdaI TGANNNNNNTCA 380 378 346 344 365 369 - TspDTI ATGAA 355 353 . . 377 380 + MseI TTAA 377 379 . . 382 386 + TspGWI ACGGA 397 395 . . 386 390 + Tsp4CI* ACNGT 388 387 . . 387 393 + TspRI CASTGNN 393 384 . . 391 395 + TfiI GAWTC 391 394 . . 391 395 + HinfI GANTC 391 394 . . 394 398 + Hpy188I TCNGA 396 395 . . 399 403 + MaeIII GTNAC 398 403 . . 409 414 + SspI AATATT 411 411 . . 418 422 + BlsI GCNGC 420 419 . . 418 422 + BisI GCNGC 419 420 . . 418 422 + TseI GCWGC 418 421 . . 418 422 - BbvI GCAGC 405 409 . . 419 424 - EcoP15I CAGCAG 391 393 . . 426 430 + BsrI ACTGG 431 429 . . 443 448 + TatI WGTACW 443 447 . . 444 447 + Csp6I GTAC 444 446 . . 444 447 + RsaI GTAC 445 445 . . 451 456 + Hpy166II GTNNAC 453 453 . . 451 456 + HpaI GTTAAC 453 453 . . 451 456 + HindII GTYRAC 453 453 . . 452 455 + MseI TTAA 452 454 . . 458 462 + TspDTI ATGAA 473 471 . . 460 464 + MboII GAAGA 472 471 . . 473 478 + MluI ACGCGT 473 477 . . 473 478 + AflIII ACRYGT 473 477 . . 474 477 + FnuDII* CGCG 475 475 . . 483 486 + SetI ASST 486 482 . . 481 485 - Hin4II* CCTTC 475 474 . . 487 492 + Hpy166II GTNNAC 489 489 . . 491 495 + Tsp4CI* ACNGT 493 492 . . 501 506 + DsaI* CCRYGG 501 505 . . 501 506 + SecI* CCNNGG 501 505 . . 500 504 - BceAI ACGGC 485 487 . . 506 509 - AciI CCGC 506 508 . . 514 519 + Cac8I GCNNGC 516 516 . . 524 529 + Hpy166II GTNNAC 526 526 . . 532 542 + MwoI GCNNNNNNNGC 538 535 . . 532 537 + Cfr10I RCCGGY 532 536 . . 533 536 + HpaII CCGG 533 535 . . 540 543 + CviRI* TGCA 541 541 . . 554 558 + Hpy188I TCNGA 556 555 . . 558 563 + XhoII RGATCY 558 562 . . 559 562 + DpnI GATC 560 560 . . 559 562 + BstKTI GATC 561 559 . . 559 562 + MboI GATC 558 562 . . 559 563 - BinI* GGATC 553 554 . . 575 578 + CviJI RGCY 576 576 . . 575 578 + HaeIII GGCC 576 576 . . 589 593 + HphI GGTGA 601 600 . . 603 608 + Hpy178III* TCNNGA 604 606 . . 603 608 + BetI* WCCGGW 603 607 . . 603 608 + BspMII* TCCGGA 603 607 . . 604 607 + HpaII CCGG 604 606 . . 608 612 + BceAI ACGGC 624 626 . . 614 618 + DdeI CTNAG 614 617 . . 630 633 + SetI ASST 633 629 . . 630 633 + CviJI RGCY 631 631 . . 630 633 + AluI AGCT 631 631 . . 634 638 + Hpy188I TCNGA 636 635 . . 632 637 - Eco57MI CTGRAG 617 615 . . 632 637 - Eco57I CTGAAG 617 615 . . 654 657 + SetI ASST 657 653 . . 654 657 + CviJI RGCY 655 655 . . 654 657 + AluI AGCT 655 655 . . 675 680 + Hpy178III* TCNNGA 676 678 . . 677 681 + Tsp45I GTSAC 676 681 . . 677 681 + MaeIII GTNAC 676 681 . . 682 685 + MseI TTAA 682 684 . . 686 689 + CviJI RGCY 687 687 . . 688 692 + BssKI CCNGG 687 692 . . 688 692 + BseBI CCWGG 689 690 . . 688 692 + ScrFI CCNGG 689 690 . . 688 692 + EcoRII CCWGG 687 692 . . 694 697 - MnlI CCTC 687 686 . . 705 709 - FokI GGATG 691 695 . . 705 709 - BseGI GGATG 704 702 . . 710 714 + DdeI CTNAG 710 713 . . 716 720 + Tsp4CI* ACNGT 718 717 . . 717 722 + SfeI* CTRYAG 717 721 . . 726 729 + SetI ASST 729 725 . . 727 731 + HphI GGTGA 739 738 . . 728 733 + Hpy166II GTNNAC 730 730 . . 732 735 + SetI ASST 735 731 . . 734 738 + DdeI CTNAG 734 737 . . 734 738 - BspCNI CTCAG 726 724 . . 734 738 - BseMII CTCAG 725 723 . . 750 755 + NlaIV GGNNCC 752 752 . . 758 761 + MseI TTAA 758 760 . . 771 774 + SetI ASST 774 770 . . 787 792 + SnaBI TACGTA 789 789 . . 787 792 + BsaAI YACGTR 789 789 . . 788 791 + TaiI ACGT 791 787 . . 788 791 + SetI ASST 791 787 . . 788 791 + MaeII ACGT 788 790 . . 804 809 + BetI* WCCGGW 804 808 . . 805 808 + HpaII CCGG 805 807 . . 808 816 + Tth111I GACNNNGTC 811 812 . . 809 813 + Tsp4CI* ACNGT 811 810 . . 810 816 + TspRI CASTGNN 816 807 . . 819 823 + FokI GGATG 832 836 . . 819 823 + BseGI GGATG 825 823 . . 820 830 + Hin4I GAYNNNNNVTC 811 806 843 838 820 824 - BccI CCATC 814 815 . . 820 830 - Hin4I GAYNNNNNVTC 843 838 811 806 849 852 + CviJI RGCY 850 850 . . 848 853 - TsoI TARCCA 838 836 . . 863 866 + TspEI AATT 862 866 . . 871 880 + XmnI GAANNNNTTC 875 875 . . 868 871 - AciI CCGC 868 870 . . 874 879 + SspI AATATT 876 876 . . 885 895 + MwoI GCNNNNNNNGC 891 888 . . 889 894 + Eco57MI CTGRAG 910 908 . . 889 894 + Eco57I CTGAAG 910 908 . . 893 896 + CviJI RGCY 894 894 . . 898 902 + MboII GAAGA 910 909 . . 922 926 - BslFI GGGAC 907 911 . . 926 934 + AlwNI CAGNNNCTG 931 928 . . 933 936 + CviRI* TGCA 934 934 . . 940 945 + BspLU11I* ACATGT 940 944 . . 940 945 + NspI RCATGY 944 940 . . 940 945 + AflIII ACRYGT 940 944 . . 941 944 + FatI CATG 940 944 . . 941 944 + NlaIII CATG 944 940 . . 941 944 + CviAII CATG 941 943 . . 944 949 + Hpy166II GTNNAC 946 946 . . 944 949 + SduI GDGCHC 948 944 . . 944 949 + BseSI GKGCMC 948 944 . . 944 949 + ApaLI GTGCAC 944 948 . . 944 949 + HgiAI* GWGCWC 948 944 . . 945 948 + CviRI* TGCA 946 946 . . 947 956 + MslI CAYNNNNRTG 951 951 . . 949 952 + MaeI CTAG 949 951 . . 974 977 + CviJI RGCY 975 975 . . 985 988 - MnlI CCTC 978 977 . . 988 991 + CviJI RGCY 989 989 . . 995 998 + MseI TTAA 995 997 . . 1006 1011 + BsaAI YACGTR 1008 1008 . . 1007 1010 + TaiI ACGT 1010 1006 . . 1007 1010 + SetI ASST 1010 1006 . . 1007 1010 + MaeII ACGT 1007 1009 . . 1026 1029 + FatI CATG 1025 1029 . . 1026 1029 + NlaIII CATG 1029 1025 . . 1026 1029 + CviAII CATG 1026 1028 . . 1038 1041 - MnlI CCTC 1031 1030 . . 1044 1054 + FalI AAGNNNNNCTT 1035 1030 1067 1062 1044 1054 - FalI AAGNNNNNCTT 1067 1062 1035 1030 1051 1055 - MboII GAAGA 1043 1042 . . 1052 1057 - Eco57MI CTGRAG 1037 1035 . . 1052 1057 - Eco57I CTGAAG 1037 1035 . . 1062 1065 - MnlI CCTC 1055 1054 . . 1066 1071 + HindIII AAGCTT 1066 1070 . . 1067 1070 + SetI ASST 1070 1066 . . 1067 1070 + CviJI RGCY 1068 1068 . . 1067 1070 + AluI AGCT 1068 1068 . . 1070 1073 + MseI TTAA 1070 1072 . . 1081 1085 + PleI GAGTC 1089 1090 . . 1081 1085 + MlyI GAGTC 1090 1090 . . 1081 1085 + HinfI GANTC 1081 1084 . . 1084 1088 + Hpy188I TCNGA 1086 1085 . . 1087 1092 + ApoI RAATTY 1087 1091 . . 1088 1091 + TspEI AATT 1087 1091 . . 1091 1094 + MseI TTAA 1091 1093 . . 1103 1106 + SetI ASST 1106 1102 . . 1101 1105 - Hin4II* CCTTC 1095 1094 . . 1107 1111 + Hpy188I TCNGA 1109 1108 . . 1116 1120 + FokI GGATG 1129 1133 . . 1116 1120 + BseGI GGATG 1122 1120 . . 1120 1124 + TfiI GAWTC 1120 1123 . . 1120 1124 + HinfI GANTC 1120 1123 . . 1130 1134 + Hpy188I TCNGA 1132 1131 . . 1134 1137 + SetI ASST 1137 1133 . . 1133 1136 - MnlI CCTC 1126 1125 . . 1136 1139 + Csp6I GTAC 1136 1138 . . 1136 1139 + RsaI GTAC 1137 1137 . . 1156 1161 + Hpy166II GTNNAC 1158 1158 . . 1156 1161 + HpaI GTTAAC 1158 1158 . . 1156 1161 + HindII GTYRAC 1158 1158 . . 1157 1160 + MseI TTAA 1157 1159 . . 1161 1164 + FatI CATG 1160 1164 . . 1161 1164 + NlaIII CATG 1164 1160 . . 1161 1164 + CviAII CATG 1161 1163 . . 1163 1168 + BalI TGGCCA 1165 1165 . . 1163 1168 + CfrI YGGCCR 1163 1167 . . 1164 1167 + CviJI RGCY 1165 1165 . . 1164 1167 + HaeIII GGCC 1165 1165 . . 1166 1171 + StyI CCWWGG 1166 1170 . . 1166 1171 + SecI* CCNNGG 1166 1170 . . 1171 1175 + TfiI GAWTC 1171 1174 . . 1171 1175 + HinfI GANTC 1171 1174 . . 1174 1179 + BetI* WCCGGW 1174 1178 . . 1175 1178 + HpaII CCGG 1175 1177 . . 1179 1182 + CviRI* TGCA 1180 1180 . . 1186 1196 + FalI AAGNNNNNCTT 1177 1172 1209 1204 1187 1190 + SetI ASST 1190 1186 . . 1189 1194 + Hpy166II GTNNAC 1191 1191 . . 1186 1196 - FalI AAGNNNNNCTT 1209 1204 1177 1172 1198 1208 + MwoI GCNNNNNNNGC 1204 1201 . . 1202 1207 + PvuII CAGCTG 1204 1204 . . 1202 1207 + NspBII* CMGCKG 1204 1204 . . 1203 1206 + SetI ASST 1206 1202 . . 1203 1206 + CviJI RGCY 1204 1204 . . 1203 1206 + AluI AGCT 1204 1204 . . 1199 1204 - Eco57MI CTGRAG 1184 1182 . . 1204 1214 + MwoI GCNNNNNNNGC 1210 1207 . . 1204 1208 + BlsI GCNGC 1206 1205 . . 1204 1208 + BisI GCNGC 1205 1206 . . 1204 1208 + TseI GCWGC 1204 1207 . . 1199 1204 - Eco57I CTGAAG 1184 1182 . . 1204 1208 - BbvI GCAGC 1191 1195 . . 1209 1214 + GsaI CCCAGC 1213 1209 . . 1209 1214 + BseYI CCCAGC 1209 1213 . . 1212 1215 + CviJI RGCY 1213 1213 . . 1220 1224 + MaeIII GTNAC 1219 1224 . . 1227 1238 + BstXI CCANNNNNNTGG 1234 1230 . . 1228 1237 + MslI CAYNNNNRTG 1232 1232 . . 1246 1249 + MseI TTAA 1246 1248 . . 1272 1277 + PsiI TTATAA 1274 1274 . . 1286 1290 + TspDTI ATGAA 1301 1299 . . 1288 1292 + MboII GAAGA 1300 1299 . . 1296 1301 + EciI GGCGGA 1312 1310 . . 1297 1300 - AciI CCGC 1297 1299 . . 1319 1323 + PleI GAGTC 1327 1328 . . 1319 1323 + MlyI GAGTC 1328 1328 . . 1319 1323 + HinfI GANTC 1319 1322 . . 1329 1339 + BsiYI* CCNNNNNNNGG 1335 1332 . . 1329 1339 + PflMI CCANNNNNTGG 1335 1332 . . 1339 1343 + MboII GAAGA 1351 1350 . . 1373 1378 + TsoI TARCCA 1389 1387 . . 1378 1383 + ApoI RAATTY 1378 1382 . . 1379 1382 + TspEI AATT 1378 1382 . . 1381 1386 + AsuII TTCGAA 1382 1384 . . 1382 1385 + TaqI TCGA 1382 1384 . . 1384 1388 + MboII GAAGA 1396 1395 . . 1387 1391 - BccI CCATC 1381 1382 . . 1399 1404 + BetI* WCCGGW 1399 1403 . . 1400 1403 + HpaII CCGG 1400 1402 . . 1405 1408 + TspEI AATT 1404 1408 . . 1411 1415 + Tsp45I GTSAC 1410 1415 . . 1411 1415 + MaeIII GTNAC 1410 1415 . . 1413 1419 + TspRI CASTGNN 1419 1410 . . 1414 1418 + BsrI ACTGG 1419 1417 . . 1423 1427 + MboII GAAGA 1435 1434 . . 1423 1428 + BbvII* GAAGAC 1430 1434 . . 1427 1431 + Tsp4CI* ACNGT 1429 1428 . . 1437 1442 + Hpy178III* TCNNGA 1438 1440 . . 1442 1445 + TspEI AATT 1441 1445 . . 1447 1451 + MboII GAAGA 1459 1458 . . 1455 1458 + TaiI ACGT 1458 1454 . . 1455 1458 + SetI ASST 1458 1454 . . 1455 1458 + MaeII ACGT 1455 1457 . . 1459 1463 + TfiI GAWTC 1459 1462 . . 1459 1463 + HinfI GANTC 1459 1462 . . 1461 1466 + ClaI ATCGAT 1462 1464 . . 1462 1465 + TaqI TCGA 1462 1464 . . 1463 1468 + McrI* CGRYCG 1466 1464 . . 1463 1468 + PvuI CGATCG 1466 1464 . . 1464 1467 + DpnI GATC 1465 1465 . . 1464 1467 + BstKTI GATC 1466 1464 . . 1464 1467 + MboI GATC 1463 1467 . . 1474 1477 + TspEI AATT 1473 1477 . . 1476 1480 - TspDTI ATGAA 1466 1464 . . 1480 1484 + Hpy188I TCNGA 1482 1481 . . 1486 1490 + TspDTI ATGAA 1501 1499 . . 1491 1494 + CviJI RGCY 1492 1492 . . 1488 1492 - Hin4II* CCTTC 1482 1481 . . 1493 1498 + Eco57MI CTGRAG 1514 1512 . . 1493 1498 + Eco57I CTGAAG 1514 1512 . . 1499 1502 + TaqI TCGA 1499 1501 . . 1501 1506 + EcoRV GATATC 1503 1503 . . 1505 1509 + Hpy188I TCNGA 1507 1506 . . 1516 1519 + TspEI AATT 1515 1519 . . 1521 1524 + CviRI* TGCA 1522 1522 . . #--------------------------------------- # # # AciI: # [CCGC] # 236 362 429 506 # # AflIII: # [ACRYGT] # 186 287 467 593 # # AluI: # [AGCT] # 9 24 49 54 136 329 # 413 519 # # AlwNI: # [CAGNNNCTG] # 602 931 # # ApaLI: # [GTGCAC] # 589 944 # # ApoI: # [RAATTY] # 155 291 1087 # # AsuII: # [TTCGAA] # 151 1382 # # BalI: # [TGGCCA] # 368 1165 # # BbvI: # [GCAGC] # 112 293 342 786 # # BbvII*: # [GAAGAC] # 103 126 239 1065 # # BccI: # [CCATC] # 58 152 567 756 # # Bce83I*: # [CTTGAG] # 246 1287 # # BceAI: # [ACGGC] # 139 485 909 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 346 1153 # # BetI*: # [WCCGGW] # 51 134 142 201 225 370 # 410 # # BinI*: # [GGATC] # 553 980 # # BisI: # [GCNGC] # 101 318 328 786 # # BlsI: # [GCNGC] # 102 318 327 786 # # BsaAI: # [YACGTR] # 219 525 789 # # BseBI: # [CCWGG] # 689 844 # # BseGI: # [GGATG] # 73 121 287 297 344 411 # # BseMII: # [CTCAG] # 725 808 # # BseSI: # [GKGCMC] # 585 948 # # BseYI: # [CCCAGC] # 324 1209 # # BsiI*: # [CACGAG] # 39 125 1369 # # BsiYI*: # [CCNNNNNNNGG] # 198 241 1094 # # BslFI: # [GGGAC] # 626 907 # # BspCNI: # [CTCAG] # 726 807 # # BspLU11I*: # [ACATGT] # 593 940 # # BspMII*: # [TCCGGA] # 51 552 930 # # BsrI: # [ACTGG] # 43 114 388 988 # # BssKI: # [CCNGG] # 241 446 846 # # BstKTI: # [GATC] # 67 561 905 # # BstXI: # [CCANNNNNNTGG] # 299 1234 # # Cac8I: # [GCNNGC] # 516 1017 # # CauII*: # [CCSGG] # 243 1290 # # Cfr10I: # [RCCGGY] # 532 1001 # # CfrI: # [YGGCCR] # 370 1163 # # ClaI: # [ATCGAT] # 71 1462 # # Csp6I: # [GTAC] # 397 444 692 # # CviAII: # [CATG] # 85 135 210 372 731 # # CviJI: # [RGCY] # 9 9 12 14 24 32 # 39 41 44 49 51 54 # 55 78 79 81 97 163 # 279 323 # # CviRI*: # [TGCA] # 11 12 29 71 207 234 # 234 342 393 # # DdeI: # [CTNAG] # 24 96 614 799 # # DpnI: # [GATC] # 68 560 905 # # DsaI*: # [CCRYGG] # 209 292 1032 # # EciI: # [GGCGGA] # 221 1312 # # Eco57I: # [CTGAAG] # 19 127 147 293 330 617 # # Eco57MI: # [CTGRAG] # 19 26 127 147 293 330 # 591 # # EcoP15I: # [CAGCAG] # 391 1142 # # EcoRII: # [CCWGG] # 687 846 # # EcoRV: # [GATATC] # 30 232 1271 # # EcoT22I: # [ATGCAT] # 73 1460 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 110 324 1035 # # FatI: # [CATG] # 85 135 209 373 731 # # FnuDII*: # [CGCG] # 475 1058 # # FokI: # [GGATG] # 80 141 287 297 324 404 # # GlaI: # [GCGC] # 266 1267 # # GsaI: # [CCCAGC] # 320 1213 # # GsuI: # [CTGGAG] # 26 1507 # # HaeIII: # [GGCC] # 368 576 589 # # HgiAI*: # [GWGCWC] # 585 948 # # HhaI: # [GCGC] # 267 1266 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 690 811 # # Hin4II*: # [CCTTC] # 51 387 475 620 # # Hin6I: # [GCGC] # 265 1268 # # HindII: # [GTYRAC] # 375 453 705 # # HindIII: # [AAGCTT] # 467 1066 # # HinfI: # [GANTC] # 39 51 74 134 140 148 # 257 690 # # HpaI: # [GTTAAC] # 375 453 705 # # HpaII: # [CCGG] # 48 52 71 133 142 201 # 225 291 370 # # HphI: # [GGTGA] # 138 265 336 794 # # Hpy166II: # [GTNNAC] # 33 36 37 91 204 212 # 216 342 362 # # Hpy178III*: # [TCNNGA] # 52 72 95 114 438 762 # # Hpy188I: # [TCNGA] # 23 23 25 26 80 135 # 160 261 350 450 # # Ksp632I*: # [CTCTTC] # 337 1196 # # MaeI: # [CTAG] # 584 949 # # MaeII: # [ACGT] # 16 78 84 92 170 219 # 426 448 # # MaeIII: # [GTNAC] # 82 123 150 166 191 278 # 543 # # MboI: # [GATC] # 70 558 905 # # MboII: # [GAAGA] # 16 24 39 45 51 74 # 102 115 133 239 257 438 # # McrI*: # [CGRYCG] # 67 1466 # # MluI: # [ACGCGT] # 473 1060 # # MlyI: # [GAGTC] # 205 238 1090 # # MnlI: # [CCTC] # 24 53 71 291 407 687 # # MseI: # [TTAA] # 21 66 75 75 76 78 # 89 230 237 287 299 # # MslI: # [CAYNNNNRTG] # 281 301 951 # # MwoI: # [GCNNNNNNNGC] # 6 109 313 323 353 429 # # NcoI: # [CCATGG] # 209 1324 # # NlaIII: # [CATG] # 85 135 213 369 731 # # NlaIV: # [GGNNCC] # 207 545 781 # # NspBII*: # [CMGCKG] # 103 329 1101 # # NspI: # [RCATGY] # 589 944 # # PflMI: # [CCANNNNNTGG] # 198 1335 # # PleI: # [GAGTC] # 206 238 1089 # # PsiI: # [TTATAA] # 259 1274 # # PvuI: # [CGATCG] # 67 1466 # # PvuII: # [CAGCTG] # 103 329 1101 # # RsaI: # [GTAC] # 396 445 692 # # ScrFI: # [CCNGG] # 243 446 844 # # SduI: # [GDGCHC] # 585 948 # # SecI*: # [CCNNGG] # 209 292 367 665 # # SetI: # [ASST] # 4 6 9 16 16 17 # 18 20 24 31 33 35 # 36 39 53 54 60 72 # 75 84 92 121 147 219 # 252 # # SfaNI: # [GCATC] # 29 58 256 1190 # # SfeI*: # [CTRYAG] # 236 481 816 # # SmlI: # [CTYRAG] # 225 1308 # # SnaBI: # [TACGTA] # 744 789 # # SspI: # [AATATT] # 411 465 657 # # StyI: # [CCWWGG] # 209 367 957 # # TaiI: # [ACGT] # 16 75 84 92 173 219 # 426 448 # # TaqI: # [TCGA] # 34 37 80 1382 # # TatI: # [WGTACW] # 443 1090 # # TfiI: # [GAWTC] # 51 74 134 257 288 729 # # TseI: # [GCWGC] # 100 318 329 786 # # TsoI: # [TARCCA] # 144 551 838 # # Tsp45I: # [GTSAC] # 123 166 510 734 # # Tsp4CI*: # [ACNGT] # 21 72 93 104 105 225 # 295 618 # # TspDTI: # [ATGAA] # 16 32 35 102 165 355 # 828 # # TspEI: # [AATT] # 18 26 32 37 42 225 # 255 291 607 # # TspGWI: # [ACGGA] # 397 1136 # # TspRI: # [CASTGNN] # 114 393 423 603 # # Tth111I: # [GACNNNGTC] # 722 811 # # XhoII: # [RGATCY] # 558 975 # # XmnI: # [GAANNNNTTC] # 362 513 658 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1533 # Reported_sequences: 1 # Reported_hitcount: 375 #---------------------------------------