Restriction Fragments for SAP30/YMR263W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SAP30/YMR263W on chromosome XIII from coordinates 794919 to 795524.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.15679.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 5 8 + MaeI CTAG 5 7 . . 8 11 + CviJI RGCY 9 9 . . 8 11 + HaeIII GGCC 9 9 . . 14 17 + MseI TTAA 14 16 . . 10 14 - BsrI ACTGG 10 8 . . 31 35 + TspGWI ACGGA 46 44 . . 34 38 + TfiI GAWTC 34 37 . . 34 38 + HinfI GANTC 34 37 . . 37 40 + TaqI TCGA 37 39 . . 37 42 + Hpy178III* TCNNGA 38 40 . . 41 44 - MnlI CCTC 34 33 . . 49 59 + BsiYI* CCNNNNNNNGG 55 52 . . 53 57 + DdeI CTNAG 53 56 . . 53 57 + BspCNI CTCAG 66 64 . . 53 57 + BseMII CTCAG 67 65 . . 65 69 + MaeIII GTNAC 64 69 . . 63 68 - TsoI TARCCA 53 51 . . 74 78 + MaeIII GTNAC 73 78 . . 86 90 + BlsI GCNGC 88 87 . . 86 90 + BisI GCNGC 87 88 . . 86 90 + BbvI GCAGC 98 102 . . 86 90 + TseI GCWGC 86 89 . . 87 92 + PvuII CAGCTG 89 89 . . 87 92 + NspBII* CMGCKG 89 89 . . 88 91 + SetI ASST 91 87 . . 88 91 + CviJI RGCY 89 89 . . 88 91 + AluI AGCT 89 89 . . 92 96 + MaeIII GTNAC 91 96 . . 107 111 + BlsI GCNGC 109 108 . . 107 111 + BisI GCNGC 108 109 . . 107 111 + BbvI GCAGC 119 123 . . 107 111 + TseI GCWGC 107 110 . . 152 162 + MwoI GCNNNNNNNGC 158 155 . . 175 179 + BmgT120I GGNCC 176 177 . . 175 179 + AsuI* GGNCC 175 178 . . 176 179 + CviJI RGCY 177 177 . . 176 179 + HaeIII GGCC 177 177 . . 181 184 + SetI ASST 184 180 . . 182 185 + MnlI CCTC 192 191 . . 182 192 + BsiYI* CCNNNNNNNGG 188 185 . . 186 191 + NspBII* CMGCKG 188 188 . . 183 188 - Eco57MI CTGRAG 168 166 . . 183 188 - GsuI CTGGAG 168 166 . . 188 191 - AciI CCGC 188 190 . . 188 192 - FauI CCCGC 181 183 . . 212 216 + HinfI GANTC 212 215 . . 212 216 - PleI GAGTC 206 207 . . 212 216 - MlyI GAGTC 206 206 . . 218 223 + PvuII CAGCTG 220 220 . . 218 223 + NspBII* CMGCKG 220 220 . . 219 222 + SetI ASST 222 218 . . 219 222 + CviJI RGCY 220 220 . . 219 222 + AluI AGCT 220 220 . . 220 224 + BlsI GCNGC 222 221 . . 220 224 + BisI GCNGC 221 222 . . 220 224 + TseI GCWGC 220 223 . . 222 225 + CviRI* TGCA 223 223 . . 220 224 - BbvI GCAGC 207 211 . . 221 226 - BsgI GTGCAG 206 204 . . 229 234 + EcoP15I CAGCAG 259 261 . . 233 238 + TatI WGTACW 233 237 . . 234 237 + Csp6I GTAC 234 236 . . 234 237 + RsaI GTAC 235 235 . . 239 242 + MseI TTAA 239 241 . . 243 247 + TfiI GAWTC 243 246 . . 243 247 + HinfI GANTC 243 246 . . 253 257 - BsmAI GTCTC 247 251 . . 263 267 - HphI GGTGA 255 254 . . 280 283 + DpnI GATC 281 281 . . 280 283 + BstKTI GATC 282 280 . . 280 283 + MboI GATC 279 283 . . 285 288 + Hin6I GCGC 285 287 . . 285 288 + GlaI GCGC 286 286 . . 285 288 + HhaI GCGC 287 285 . . 294 298 + PleI GAGTC 302 303 . . 294 298 + MlyI GAGTC 303 303 . . 294 298 + HinfI GANTC 294 297 . . 296 300 + Tsp45I GTSAC 295 300 . . 296 300 + MaeIII GTNAC 295 300 . . 298 303 + TaqII CACCCA 314 312 . . 297 301 - HphI GGTGA 289 288 . . 310 315 + AsuII TTCGAA 311 313 . . 311 314 + TaqI TCGA 311 313 . . 313 317 + MboII GAAGA 325 324 . . 318 323 + Hpy166II GTNNAC 320 320 . . 313 318 - Ksp632I* CTCTTC 308 311 . . 318 323 + BssNAI GTATAC 320 320 . . 318 323 + AccI GTMKAC 319 321 . . 322 326 + TspGWI ACGGA 337 335 . . 324 328 + FokI GGATG 337 341 . . 324 328 + BseGI GGATG 330 328 . . 325 329 - SfaNI GCATC 315 319 . . 332 337 + Hpy178III* TCNNGA 333 335 . . 334 338 + DdeI CTNAG 334 337 . . 334 338 - BspCNI CTCAG 326 324 . . 334 338 - BseMII CTCAG 325 323 . . 340 343 + SetI ASST 343 339 . . 338 342 - Hin4II* CCTTC 332 331 . . 358 363 + MfeI CAATTG 358 362 . . 359 362 + TspEI AATT 358 362 . . 365 368 + TaiI ACGT 368 364 . . 365 368 + SetI ASST 368 364 . . 365 368 + MaeII ACGT 365 367 . . 367 370 + Csp6I GTAC 367 369 . . 367 370 + RsaI GTAC 368 368 . . 369 374 + BetI* WCCGGW 369 373 . . 370 373 + HpaII CCGG 370 372 . . 377 380 + SetI ASST 380 376 . . 381 385 + HgaI GACGC 390 395 . . 384 388 + BlsI GCNGC 386 385 . . 384 388 + BisI GCNGC 385 386 . . 384 388 + TseI GCWGC 384 387 . . 385 390 + PstI CTGCAG 389 385 . . 385 390 + SfeI* CTRYAG 385 389 . . 386 389 + CviRI* TGCA 387 387 . . 384 388 - BbvI GCAGC 371 375 . . 391 396 - BciVI GTATCC 385 384 . . 403 408 + NlaIV GGNNCC 405 405 . . 410 413 + TspEI AATT 409 413 . . 416 419 + Hin6I GCGC 416 418 . . 416 419 + GlaI GCGC 417 417 . . 416 419 + HhaI GCGC 418 416 . . 417 420 + FnuDII* CGCG 418 418 . . 420 424 + MboII GAAGA 432 431 . . 420 425 + BbvII* GAAGAC 427 431 . . 429 433 - TspDTI ATGAA 419 417 . . 438 441 - MnlI CCTC 431 430 . . 453 457 + BlsI GCNGC 455 454 . . 453 457 + BisI GCNGC 454 455 . . 453 457 + BbvI GCAGC 465 469 . . 453 457 + TseI GCWGC 453 456 . . 467 472 - BciVI GTATCC 461 460 . . 482 485 + SetI ASST 485 481 . . 483 487 + BssKI CCNGG 482 487 . . 483 487 + BseBI CCWGG 484 485 . . 483 487 + ScrFI CCNGG 484 485 . . 479 483 - BsmAI GTCTC 473 477 . . 483 487 + EcoRII CCWGG 482 487 . . 479 484 - Eco31I GGTCTC 473 477 . . 485 490 + BalI TGGCCA 487 487 . . 485 490 + CfrI YGGCCR 485 489 . . 486 489 + CviJI RGCY 487 487 . . 486 489 + HaeIII GGCC 487 487 . . 491 494 + TaiI ACGT 494 490 . . 491 494 + SetI ASST 494 490 . . 491 494 + MaeII ACGT 491 493 . . 495 499 + HphI GGTGA 507 506 . . 500 504 - Hin4II* CCTTC 494 493 . . 505 514 + MslI CAYNNNNRTG 509 509 . . 509 512 + TaqI TCGA 509 511 . . 516 521 - BsmI GAATGC 516 514 . . 524 527 + MseI TTAA 524 526 . . 529 533 - BsmAI GTCTC 523 527 . . 538 541 + CviRI* TGCA 539 539 . . 544 547 + AciI CCGC 544 546 . . 548 551 + TspEI AATT 547 551 . . 560 563 + SetI ASST 563 559 . . 573 577 + MboII GAAGA 585 584 . . 576 580 + MboII GAAGA 588 587 . . 576 585 + XmnI GAANNNNTTC 580 580 . . 580 585 + ApoI RAATTY 580 584 . . 581 584 + TspEI AATT 580 584 . . 584 589 + Hpy178III* TCNNGA 585 587 . . 588 592 - BccI CCATC 582 583 . . 592 597 + ApoI RAATTY 592 596 . . 593 596 + TspEI AATT 592 596 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 287 319 # # AciI: # [CCGC] # 62 188 356 # # AluI: # [AGCT] # 89 131 386 # # ApoI: # [RAATTY] # 12 14 580 # # AsuI*: # [GGNCC] # 175 431 # # AsuII: # [TTCGAA] # 295 311 # # BalI: # [TGGCCA] # 119 487 # # BbvI: # [GCAGC] # 21 88 94 98 141 164 # # BbvII*: # [GAAGAC] # 179 427 # # BccI: # [CCATC] # 24 582 # # BciVI: # [GTATCC] # 76 145 385 # # BetI*: # [WCCGGW] # 237 369 # # BisI: # [GCNGC] # 21 69 87 113 152 164 # # BlsI: # [GCNGC] # 21 69 88 113 151 164 # # BmgT120I: # [GGNCC] # 176 430 # # BseBI: # [CCWGG] # 122 484 # # BseGI: # [GGATG] # 276 330 # # BseMII: # [CTCAG] # 67 258 281 # # BsgI: # [GTGCAG] # 206 400 # # BsiYI*: # [CCNNNNNNNGG] # 55 133 418 # # BsmAI: # [GTCTC] # 50 83 226 247 # # BsmI: # [GAATGC] # 90 516 # # BspCNI: # [CTCAG] # 66 260 280 # # BsrI: # [ACTGG] # 10 596 # # BssKI: # [CCNGG] # 124 482 # # BssNAI: # [GTATAC] # 286 320 # # BstKTI: # [GATC] # 282 324 # # CfrI: # [YGGCCR] # 121 485 # # Csp6I: # [GTAC] # 133 234 239 # # CviJI: # [RGCY] # 4 5 43 80 88 119 # 267 # # CviRI*: # [TGCA] # 67 152 164 223 # # DdeI: # [CTNAG] # 53 272 281 # # DpnI: # [GATC] # 281 325 # # Eco31I: # [GGTCTC] # 133 473 # # Eco57MI: # [CTGRAG] # 168 438 # # EcoP15I: # [CAGCAG] # 259 347 # # EcoRII: # [CCWGG] # 124 482 # # FauI: # [CCCGC] # 181 425 # # FnuDII*: # [CGCG] # 188 418 # # FokI: # [GGATG] # 269 337 # # GlaI: # [GCGC] # 131 189 286 # # GsuI: # [CTGGAG] # 168 438 # # HaeIII: # [GGCC] # 9 119 168 310 # # HgaI: # [GACGC] # 216 390 # # HhaI: # [GCGC] # 131 188 287 # # Hin4II*: # [CCTTC] # 112 162 332 # # Hin6I: # [GCGC] # 131 190 285 # # HinfI: # [GANTC] # 31 34 51 178 312 # # HpaII: # [CCGG] # 236 370 # # HphI: # [GGTGA] # 34 99 218 255 # # Hpy166II: # [GTNNAC] # 286 320 # # Hpy178III*: # [TCNNGA] # 21 38 252 295 # # Ksp632I*: # [CTCTTC] # 298 308 # # MaeI: # [CTAG] # 5 601 # # MaeII: # [ACGT] # 115 126 365 # # MaeIII: # [GTNAC] # 9 18 64 204 311 # # MboI: # [GATC] # 279 327 # # MboII: # [GAAGA] # 3 18 107 153 325 # # MfeI: # [CAATTG] # 248 358 # # MlyI: # [GAGTC] # 97 206 303 # # MnlI: # [CCTC] # 34 158 175 239 # # MseI: # [TTAA] # 14 82 225 285 # # MslI: # [CAYNNNNRTG] # 97 509 # # MwoI: # [GCNNNNNNNGC] # 158 448 # # NlaIV: # [GGNNCC] # 201 405 # # NspBII*: # [CMGCKG] # 32 89 99 386 # # PleI: # [GAGTC] # 96 206 304 # # PstI: # [CTGCAG] # 217 389 # # PvuII: # [CAGCTG] # 89 131 386 # # RsaI: # [GTAC] # 133 235 238 # # ScrFI: # [CCNGG] # 122 484 # # SetI: # [ASST] # 9 12 25 38 43 69 # 91 93 105 121 # # SfaNI: # [GCATC] # 291 315 # # SfeI*: # [CTRYAG] # 221 385 # # TaiI: # [ACGT] # 112 126 368 # # TaqI: # [TCGA] # 37 97 198 274 # # TaqII: # [CACCCA] # 292 314 # # TatI: # [WGTACW] # 233 373 # # TfiI: # [GAWTC] # 34 209 363 # # TseI: # [GCWGC] # 21 69 86 113 153 164 # # TsoI: # [TARCCA] # 53 553 # # Tsp45I: # [GTSAC] # 295 311 # # TspDTI: # [ATGAA] # 187 419 # # TspEI: # [AATT] # 12 14 33 51 138 358 # # TspGWI: # [ACGGA] # 46 269 291 # # XmnI: # [GAANNNNTTC] # 26 580 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 606 # Reported_sequences: 1 # Reported_hitcount: 167 #---------------------------------------