Restriction Fragments for BCH1/YMR237W



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The currently selected gene/sequence is :

BCH1/YMR237W on chromosome XIII from coordinates 743749 to 745923.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22475.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + BsmAI GTCTC 11 15 . . 13 16 + SetI ASST 16 12 . . 14 17 + MnlI CCTC 24 23 . . 21 26 + BetI* WCCGGW 21 25 . . 22 25 + HpaII CCGG 22 24 . . 34 38 + MboII GAAGA 46 45 . . 34 39 + BbvII* GAAGAC 41 45 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 37 42 - BtrI CACGTC 39 39 . . 46 49 + CviJI RGCY 47 47 . . 52 55 + Hin6I GCGC 52 54 . . 52 55 + GlaI GCGC 53 53 . . 52 55 + HhaI GCGC 54 52 . . 63 68 + BseRI GAGGAG 78 76 . . 63 66 - MnlI CCTC 56 55 . . 69 72 + CviJI RGCY 70 70 . . 78 82 + BslFI GGGAC 92 96 . . 83 86 + TspEI AATT 82 86 . . 87 97 + BsiYI* CCNNNNNNNGG 93 90 . . 87 97 + PflMI CCANNNNNTGG 93 90 . . 91 100 + PshAI GACNNNNGTC 95 95 . . 96 101 + NlaIV GGNNCC 98 98 . . 97 101 + BmgT120I GGNCC 98 99 . . 97 101 + AvaII GGWCC 97 100 . . 97 101 + AsuI* GGNCC 97 100 . . 100 103 + AciI CCGC 100 102 . . 99 104 - EciI GGCGGA 89 87 . . 109 112 + MseI TTAA 109 111 . . 111 114 + TspEI AATT 110 114 . . 119 124 + BclI TGATCA 119 123 . . 120 123 + DpnI GATC 121 121 . . 120 123 + BstKTI GATC 122 120 . . 120 123 + MboI GATC 119 123 . . 129 133 + MaeIII GTNAC 128 133 . . 132 135 + SetI ASST 135 131 . . 133 137 + Hin4II* CCTTC 143 142 . . 135 139 - TspDTI ATGAA 125 123 . . 140 147 + PspXI VCTCGAGB 141 145 . . 140 143 + MnlI CCTC 150 149 . . 141 146 + SmlI CTYRAG 141 145 . . 141 146 + XhoI CTCGAG 141 145 . . 137 141 - FokI GGATG 123 127 . . 137 141 - BseGI GGATG 136 134 . . 141 146 + BmeT110I CYCGRG 142 144 . . 141 146 + AvaI CYCGRG 141 145 . . 142 145 + TaqI TCGA 142 144 . . 144 149 + SduI GDGCHC 148 144 . . 144 149 + HgiAI* GWGCWC 148 144 . . 164 167 + MnlI CCTC 174 173 . . 167 170 + AciI CCGC 167 169 . . 166 171 - EciI GGCGGA 156 154 . . 179 183 + TspGWI ACGGA 194 192 . . 207 212 + XhoII RGATCY 207 211 . . 208 211 + DpnI GATC 209 209 . . 208 211 + BstKTI GATC 210 208 . . 208 211 + MboI GATC 207 211 . . 208 212 - BinI* GGATC 202 203 . . 223 227 + MaeIII GTNAC 222 227 . . 226 230 + TspGWI ACGGA 241 239 . . 231 235 + MaeIII GTNAC 230 235 . . 235 244 + MslI CAYNNNNRTG 239 239 . . 241 244 + FatI CATG 240 244 . . 241 244 + NlaIII CATG 244 240 . . 241 244 + CviAII CATG 241 243 . . 248 251 + TspEI AATT 247 251 . . 250 253 + MseI TTAA 250 252 . . 261 264 + DpnI GATC 262 262 . . 261 264 + BstKTI GATC 263 261 . . 261 264 + MboI GATC 260 264 . . 278 282 + Tsp4CI* ACNGT 280 279 . . 289 294 + ClaI ATCGAT 290 292 . . 290 293 + TaqI TCGA 290 292 . . 298 302 + Hpy188I TCNGA 300 299 . . 287 299 - BarI GAAGNNNNNNTAC 311 306 279 274 300 305 + XhoII RGATCY 300 304 . . 300 305 + NlaIV GGNNCC 302 302 . . 300 305 + BamHI GGATCC 300 304 . . 300 304 + BinI* GGATC 308 309 . . 301 304 + DpnI GATC 302 302 . . 301 304 + BstKTI GATC 303 301 . . 301 304 + MboI GATC 300 304 . . 303 308 + MmeI TCCRAC 328 326 . . 301 305 - BinI* GGATC 295 296 . . 333 343 + Hin4I GAYNNNNNVTC 324 319 356 351 340 343 + DpnI GATC 341 341 . . 340 343 + BstKTI GATC 342 340 . . 340 343 + MboI GATC 339 343 . . 333 343 - Hin4I GAYNNNNNVTC 356 351 324 319 348 352 + DdeI CTNAG 348 351 . . 359 369 + BsiYI* CCNNNNNNNGG 365 362 . . 360 374 + XcmI CCANNNNNNNNNTGG 367 366 . . 362 367 + ApoI RAATTY 362 366 . . 363 366 + TspEI AATT 362 366 . . 378 382 + MboII GAAGA 390 389 . . 379 389 + FalI AAGNNNNNCTT 370 365 402 397 379 389 - FalI AAGNNNNNCTT 402 397 370 365 391 396 + BsaAI YACGTR 393 393 . . 392 395 + TaiI ACGT 395 391 . . 392 395 + SetI ASST 395 391 . . 392 395 + MaeII ACGT 392 394 . . 397 400 + TaqI TCGA 397 399 . . 399 403 + MboII GAAGA 411 410 . . 402 411 + BsaBI GATNNNNATC 406 406 . . 420 425 + BsmI GAATGC 426 424 . . 442 447 + Hpy166II GTNNAC 444 444 . . 442 447 + HpaI GTTAAC 444 444 . . 442 447 + HindII GTYRAC 444 444 . . 443 446 + MseI TTAA 443 445 . . 454 459 + Hpy166II GTNNAC 456 456 . . 462 468 + PfoI TCCNGGA 462 467 . . 463 467 + BssKI CCNGG 462 467 . . 463 467 + BseBI CCWGG 464 465 . . 463 467 + ScrFI CCNGG 464 465 . . 463 467 + EcoRII CCWGG 462 467 . . 464 472 + AlwNI CAGNNNCTG 469 466 . . 469 473 + Tsp4CI* ACNGT 471 470 . . 473 476 + CviRI* TGCA 474 474 . . 478 481 + SetI ASST 481 477 . . 485 490 + PsiI TTATAA 487 487 . . 495 499 + Hpy99I CGWCG 499 494 . . 497 501 + Tsp4CI* ACNGT 499 498 . . 499 503 + HphI GGTGA 511 510 . . 500 505 + Hpy166II GTNNAC 502 502 . . 518 521 + MnlI CCTC 528 527 . . 521 526 + DsaI* CCRYGG 521 525 . . 521 526 + SecI* CCNNGG 521 525 . . 520 524 - TspGWI ACGGA 510 508 . . 532 536 - HgaI GACGC 521 526 . . 540 545 + CfrI YGGCCR 540 544 . . 541 544 + CviJI RGCY 542 542 . . 541 544 + HaeIII GGCC 542 542 . . 542 546 + TauI GCSGC 545 542 . . 542 546 + BlsI GCNGC 544 543 . . 542 546 + BisI GCNGC 543 544 . . 543 546 + AciI CCGC 543 545 . . 558 563 + AhaIII* TTTAAA 560 560 . . 559 562 + MseI TTAA 559 561 . . 563 566 + TaiI ACGT 566 562 . . 563 566 + SetI ASST 566 562 . . 563 566 + MaeII ACGT 563 565 . . 565 570 + Hpy166II GTNNAC 567 567 . . 565 570 + HindII GTYRAC 567 567 . . 570 573 + FatI CATG 569 573 . . 570 573 + NlaIII CATG 573 569 . . 570 573 + CviAII CATG 570 572 . . 579 584 + EciI GGCGGA 595 593 . . 580 583 - AciI CCGC 580 582 . . 585 590 + AclI AACGTT 586 588 . . 586 589 + TaiI ACGT 589 585 . . 586 589 + SetI ASST 589 585 . . 586 589 + MaeII ACGT 586 588 . . 604 607 + Csp6I GTAC 604 606 . . 604 607 + RsaI GTAC 605 605 . . 605 610 + BsaAI YACGTR 607 607 . . 606 609 + TaiI ACGT 609 605 . . 606 609 + SetI ASST 609 605 . . 606 609 + MaeII ACGT 606 608 . . 608 612 + Tsp45I GTSAC 607 612 . . 608 612 + MaeIII GTNAC 607 612 . . 622 626 + TspDTI ATGAA 637 635 . . 639 643 + FokI GGATG 652 656 . . 639 643 + BseGI GGATG 645 643 . . 640 650 + Hin4I GAYNNNNNVTC 631 626 663 658 637 640 - AciI CCGC 637 639 . . 640 644 - BccI CCATC 634 635 . . 646 650 + TfiI GAWTC 646 649 . . 646 650 + HinfI GANTC 646 649 . . 640 650 - Hin4I GAYNNNNNVTC 663 658 631 626 651 661 + BsiYI* CCNNNNNNNGG 657 654 . . 651 661 + PflMI CCANNNNNTGG 657 654 . . 654 659 + ApoI RAATTY 654 658 . . 655 658 + TspEI AATT 654 658 . . 672 675 + TspEI AATT 671 675 . . 688 691 + SetI ASST 691 687 . . 689 692 + MnlI CCTC 699 698 . . 689 694 + SecI* CCNNGG 689 693 . . 690 695 + BmeT110I CYCGRG 691 693 . . 690 695 + AvaI CYCGRG 690 694 . . 694 698 + HphI GGTGA 706 705 . . 703 707 + MboII GAAGA 715 714 . . 699 704 - MmeI TCCRAC 680 678 . . 725 728 + MseI TTAA 725 727 . . 729 733 + Tsp4CI* ACNGT 731 730 . . 737 742 + StyI CCWWGG 737 741 . . 737 742 + SecI* CCNNGG 737 741 . . 746 750 + TspDTI ATGAA 761 759 . . 753 756 + SetI ASST 756 752 . . 768 771 + CviRI* TGCA 769 769 . . 766 770 - SfaNI GCATC 756 760 . . 771 774 + SetI ASST 774 770 . . 779 782 + TaiI ACGT 782 778 . . 779 782 + SetI ASST 782 778 . . 779 782 + MaeII ACGT 779 781 . . 783 788 + AhaIII* TTTAAA 785 785 . . 784 787 + MseI TTAA 784 786 . . 791 794 + MnlI CCTC 801 800 . . 795 800 + Hpy166II GTNNAC 797 797 . . 795 800 + HpaI GTTAAC 797 797 . . 795 800 + HindII GTYRAC 797 797 . . 796 799 + MseI TTAA 796 798 . . 797 802 + TsoI TARCCA 813 811 . . 805 808 + TspEI AATT 804 808 . . 838 841 + SetI ASST 841 837 . . 840 845 + Hpy166II GTNNAC 842 842 . . 840 845 + HpaI GTTAAC 842 842 . . 840 845 + HindII GTYRAC 842 842 . . 841 844 + MseI TTAA 841 843 . . 845 849 + DdeI CTNAG 845 848 . . 859 864 + NspI RCATGY 863 859 . . 860 863 + FatI CATG 859 863 . . 860 863 + NlaIII CATG 863 859 . . 860 863 + CviAII CATG 860 862 . . 874 879 + SmlI CTYRAG 874 878 . . 875 880 + Hpy178III* TCNNGA 876 878 . . 874 879 - Bce83I* CTTGAG 859 857 . . 883 886 + MseI TTAA 883 885 . . 890 895 + ApoI RAATTY 890 894 . . 891 894 + TspEI AATT 890 894 . . 893 897 - TspDTI ATGAA 883 881 . . 897 902 + Hpy178III* TCNNGA 898 900 . . 895 899 - FokI GGATG 881 885 . . 895 899 - BseGI GGATG 894 892 . . 912 917 + SspI AATATT 914 914 . . 915 920 + VspI ATTAAT 916 918 . . 916 919 + MseI TTAA 916 918 . . 918 921 + TspEI AATT 917 921 . . 934 939 + Cac8I GCNNGC 936 936 . . 954 957 + CviRI* TGCA 955 955 . . 952 956 - SfaNI GCATC 942 946 . . 958 963 + Hpy166II GTNNAC 960 960 . . 958 963 + HindII GTYRAC 960 960 . . 962 965 + SetI ASST 965 961 . . 965 970 + BclI TGATCA 965 969 . . 966 969 + DpnI GATC 967 967 . . 966 969 + BstKTI GATC 968 966 . . 966 969 + MboI GATC 965 969 . . 981 986 + ApoI RAATTY 981 985 . . 982 985 + TspEI AATT 981 985 . . 989 992 + MnlI CCTC 999 998 . . 990 995 + Ksp632I* CTCTTC 996 999 . . 991 995 - MboII GAAGA 983 982 . . 999 1003 + DdeI CTNAG 999 1002 . . 1002 1005 + CviJI RGCY 1003 1003 . . 1005 1009 + Hpy99I CGWCG 1009 1004 . . 1009 1013 + TfiI GAWTC 1009 1012 . . 1009 1013 + HinfI GANTC 1009 1012 . . 1019 1022 + MaeI CTAG 1019 1021 . . 1020 1025 + TsoI TARCCA 1036 1034 . . 1021 1024 + CviJI RGCY 1022 1022 . . 1031 1034 + SetI ASST 1034 1030 . . 1031 1034 + CviJI RGCY 1032 1032 . . 1031 1034 + AluI AGCT 1032 1032 . . 1037 1041 - MboII GAAGA 1029 1028 . . 1045 1049 + Hpy188I TCNGA 1047 1046 . . 1049 1052 + TspEI AATT 1048 1052 . . 1054 1057 + MseI TTAA 1054 1056 . . 1069 1074 + EcoRI GAATTC 1069 1073 . . 1069 1074 + ApoI RAATTY 1069 1073 . . 1070 1073 + TspEI AATT 1069 1073 . . 1077 1081 + BsrI ACTGG 1082 1080 . . 1080 1084 + FokI GGATG 1093 1097 . . 1080 1084 + BseGI GGATG 1086 1084 . . 1085 1090 + Hpy178III* TCNNGA 1086 1088 . . 1099 1104 + HindIII AAGCTT 1099 1103 . . 1100 1103 + SetI ASST 1103 1099 . . 1100 1103 + CviJI RGCY 1101 1101 . . 1100 1103 + AluI AGCT 1101 1101 . . 1101 1106 + Cac8I GCNNGC 1103 1103 . . 1106 1110 + DdeI CTNAG 1106 1109 . . 1105 1111 - Bpu10I CCTNAGC 1106 1109 . . 1115 1120 + TsoI TARCCA 1131 1129 . . 1116 1119 + CviJI RGCY 1117 1117 . . 1117 1127 + MwoI GCNNNNNNNGC 1123 1120 . . 1117 1127 + BglI GCCNNNNNGGC 1123 1120 . . 1118 1123 + DsaI* CCRYGG 1118 1122 . . 1118 1123 + StyI CCWWGG 1118 1122 . . 1118 1123 + NcoI CCATGG 1118 1122 . . 1118 1123 + SecI* CCNNGG 1118 1122 . . 1119 1122 + FatI CATG 1118 1122 . . 1119 1122 + NlaIII CATG 1122 1118 . . 1119 1122 + CviAII CATG 1119 1121 . . 1125 1128 + CviJI RGCY 1126 1126 . . 1123 1126 - MnlI CCTC 1116 1115 . . 1129 1134 + Hpy166II GTNNAC 1131 1131 . . 1129 1134 + HpaI GTTAAC 1131 1131 . . 1129 1134 + HindII GTYRAC 1131 1131 . . 1130 1133 + MseI TTAA 1130 1132 . . 1133 1137 + Tsp4CI* ACNGT 1135 1134 . . 1136 1141 + Hpy166II GTNNAC 1138 1138 . . 1136 1141 + SduI GDGCHC 1140 1136 . . 1136 1141 + BseSI GKGCMC 1140 1136 . . 1136 1141 + ApaLI GTGCAC 1136 1140 . . 1136 1141 + HgiAI* GWGCWC 1140 1136 . . 1137 1140 + CviRI* TGCA 1138 1138 . . 1147 1152 + ApoI RAATTY 1147 1151 . . 1148 1151 + TspEI AATT 1147 1151 . . 1150 1155 + AhaIII* TTTAAA 1152 1152 . . 1151 1154 + MseI TTAA 1151 1153 . . 1172 1175 + MaeI CTAG 1172 1174 . . 1184 1187 + MseI TTAA 1184 1186 . . 1219 1222 + MseI TTAA 1219 1221 . . 1225 1230 + Cac8I GCNNGC 1227 1227 . . 1233 1236 + FatI CATG 1232 1236 . . 1233 1236 + NlaIII CATG 1236 1232 . . 1233 1236 + CviAII CATG 1233 1235 . . 1236 1240 + Tsp45I GTSAC 1235 1240 . . 1236 1240 + MaeIII GTNAC 1235 1240 . . 1241 1244 + SetI ASST 1244 1240 . . 1244 1247 + MseI TTAA 1244 1246 . . 1256 1259 + TaiI ACGT 1259 1255 . . 1256 1259 + SetI ASST 1259 1255 . . 1256 1259 + MaeII ACGT 1256 1258 . . 1261 1265 + DdeI CTNAG 1261 1264 . . 1265 1269 + MboII GAAGA 1277 1276 . . 1269 1272 + TspEI AATT 1268 1272 . . 1273 1276 + Hin6I GCGC 1273 1275 . . 1273 1276 + GlaI GCGC 1274 1274 . . 1273 1276 + HhaI GCGC 1275 1273 . . 1285 1289 + Hpy188I TCNGA 1287 1286 . . 1283 1288 - Eco57MI CTGRAG 1268 1266 . . 1288 1297 + BsaBI GATNNNNATC 1292 1292 . . 1283 1288 - Eco57I CTGAAG 1268 1266 . . 1289 1293 + TspDTI ATGAA 1304 1302 . . 1290 1301 + BdaI TGANNNNNNTCA 1279 1277 1313 1311 1291 1300 + XmnI GAANNNNTTC 1295 1295 . . 1296 1300 - MboII GAAGA 1288 1287 . . 1290 1301 - BdaI TGANNNNNNTCA 1313 1311 1279 1277 1298 1302 - TspDTI ATGAA 1288 1286 . . 1317 1321 + FokI GGATG 1330 1334 . . 1317 1321 + BseGI GGATG 1323 1321 . . 1322 1325 + MnlI CCTC 1332 1331 . . 1318 1322 - SfaNI GCATC 1308 1312 . . 1325 1330 + MfeI CAATTG 1325 1329 . . 1326 1329 + TspEI AATT 1325 1329 . . 1330 1335 + BseRI GAGGAG 1345 1343 . . 1330 1333 - MnlI CCTC 1323 1322 . . 1332 1342 - BsaXI ACNNNNNCTCC 1354 1351 1324 1321 1333 1343 - Hin4I GAYNNNNNVTC 1356 1351 1324 1319 1352 1357 + DsaI* CCRYGG 1352 1356 . . 1352 1357 + StyI CCWWGG 1352 1356 . . 1352 1357 + NcoI CCATGG 1352 1356 . . 1352 1357 + SecI* CCNNGG 1352 1356 . . 1353 1356 + FatI CATG 1352 1356 . . 1353 1356 + NlaIII CATG 1356 1352 . . 1353 1356 + CviAII CATG 1353 1355 . . 1356 1360 + FokI GGATG 1369 1373 . . 1356 1360 + BseGI GGATG 1362 1360 . . 1362 1366 - BsrI ACTGG 1362 1360 . . 1365 1370 - MmeI TCCRAC 1346 1344 . . 1368 1378 - BsaXI ACNNNNNCTCC 1390 1387 1360 1357 1369 1379 - Hin4I GAYNNNNNVTC 1392 1387 1360 1355 1379 1382 + AciI CCGC 1379 1381 . . 1383 1388 + XhoII RGATCY 1383 1387 . . 1384 1387 + DpnI GATC 1385 1385 . . 1384 1387 + BstKTI GATC 1386 1384 . . 1384 1387 + MboI GATC 1383 1387 . . 1384 1388 - BinI* GGATC 1378 1379 . . 1391 1394 + SetI ASST 1394 1390 . . 1393 1396 + MaeI CTAG 1393 1395 . . 1408 1412 - HgaI GACGC 1397 1402 . . 1416 1421 + AhaIII* TTTAAA 1418 1418 . . 1417 1420 + MseI TTAA 1417 1419 . . 1422 1427 + Hpy166II GTNNAC 1424 1424 . . 1422 1427 + AccI GTMKAC 1423 1425 . . 1430 1435 + MmeI TCCRAC 1455 1453 . . 1435 1438 + MaeI CTAG 1435 1437 . . 1437 1440 + CviJI RGCY 1438 1438 . . 1445 1448 + TspEI AATT 1444 1448 . . 1458 1461 + TspEI AATT 1457 1461 . . 1474 1478 + FokI GGATG 1487 1491 . . 1474 1478 + BseGI GGATG 1480 1478 . . 1475 1479 - BccI CCATC 1469 1470 . . 1482 1486 + BlsI GCNGC 1484 1483 . . 1482 1486 + BisI GCNGC 1483 1484 . . 1482 1486 + BbvI GCAGC 1494 1498 . . 1482 1486 + TseI GCWGC 1482 1485 . . 1483 1488 + PvuII CAGCTG 1485 1485 . . 1483 1488 + NspBII* CMGCKG 1485 1485 . . 1484 1487 + SetI ASST 1487 1483 . . 1484 1487 + CviJI RGCY 1485 1485 . . 1484 1487 + AluI AGCT 1485 1485 . . 1479 1489 - BsaXI ACNNNNNCTCC 1501 1498 1471 1468 1499 1502 + DpnI GATC 1500 1500 . . 1499 1502 + BstKTI GATC 1501 1499 . . 1499 1502 + MboI GATC 1498 1502 . . 1505 1510 + ApoI RAATTY 1505 1509 . . 1506 1509 + TspEI AATT 1505 1509 . . 1519 1523 + MboII GAAGA 1531 1530 . . 1519 1524 + BbvII* GAAGAC 1526 1530 . . 1527 1530 + Csp6I GTAC 1527 1529 . . 1527 1530 + RsaI GTAC 1528 1528 . . 1529 1539 + BsaXI ACNNNNNCTCC 1519 1516 1549 1546 1530 1535 + StyI CCWWGG 1530 1534 . . 1530 1535 + SecI* CCNNGG 1530 1534 . . 1534 1537 + CviJI RGCY 1535 1535 . . 1534 1539 + NlaIV GGNNCC 1536 1536 . . 1543 1547 - MboII GAAGA 1535 1534 . . 1553 1557 + TspDTI ATGAA 1568 1566 . . 1567 1570 + FnuDII* CGCG 1568 1568 . . 1568 1571 - AciI CCGC 1568 1570 . . 1592 1595 + DpnI GATC 1593 1593 . . 1592 1595 + BstKTI GATC 1594 1592 . . 1592 1595 + MboI GATC 1591 1595 . . 1601 1604 + CviJI RGCY 1602 1602 . . 1599 1602 - MnlI CCTC 1592 1591 . . 1613 1617 - BccI CCATC 1607 1608 . . 1617 1622 - MmeI TCCRAC 1598 1596 . . 1627 1632 + Ksp632I* CTCTTC 1633 1636 . . 1628 1632 - MboII GAAGA 1620 1619 . . 1632 1635 + FatI CATG 1631 1635 . . 1632 1635 + NlaIII CATG 1635 1631 . . 1632 1635 + CviAII CATG 1632 1634 . . 1630 1634 - TspDTI ATGAA 1620 1618 . . 1635 1639 + MaeIII GTNAC 1634 1639 . . 1645 1649 + MboII GAAGA 1657 1656 . . 1666 1676 + Hin4I GAYNNNNNVTC 1657 1652 1689 1684 1671 1681 + MwoI GCNNNNNNNGC 1677 1674 . . 1675 1679 + Hpy188I TCNGA 1677 1676 . . 1666 1676 - Hin4I GAYNNNNNVTC 1689 1684 1657 1652 1681 1686 + MfeI CAATTG 1681 1685 . . 1682 1685 + TspEI AATT 1681 1685 . . 1693 1697 - SfaNI GCATC 1683 1687 . . 1703 1707 + Tsp4CI* ACNGT 1705 1704 . . 1718 1721 + TspEI AATT 1717 1721 . . 1720 1723 + MseI TTAA 1720 1722 . . 1723 1727 + Tsp4CI* ACNGT 1725 1724 . . 1736 1739 + TspEI AATT 1735 1739 . . 1744 1747 + CviJI RGCY 1745 1745 . . 1744 1747 + HaeIII GGCC 1745 1745 . . 1761 1765 + BsrI ACTGG 1766 1764 . . 1761 1766 + BfiI ACTGGG 1771 1770 . . 1764 1768 + BslFI GGGAC 1778 1782 . . 1773 1778 + Hpy178III* TCNNGA 1774 1776 . . 1770 1774 - MboII GAAGA 1762 1761 . . 1775 1783 + AlwNI CAGNNNCTG 1780 1777 . . 1779 1782 + SetI ASST 1782 1778 . . 1779 1782 + CviJI RGCY 1780 1780 . . 1779 1782 + AluI AGCT 1780 1780 . . 1780 1790 + MwoI GCNNNNNNNGC 1786 1783 . . 1780 1784 + BlsI GCNGC 1782 1781 . . 1780 1784 + BisI GCNGC 1781 1782 . . 1780 1784 + TseI GCWGC 1780 1783 . . 1780 1784 - BbvI GCAGC 1767 1771 . . 1785 1794 + XmnI GAANNNNTTC 1789 1789 . . 1788 1791 + CviJI RGCY 1789 1789 . . 1785 1789 - Hin4II* CCTTC 1779 1778 . . 1797 1800 + TspEI AATT 1796 1800 . . 1802 1805 + CviJI RGCY 1803 1803 . . 1820 1825 - Eco57MI CTGRAG 1805 1803 . . 1820 1825 - GsuI CTGGAG 1805 1803 . . 1822 1826 - BsrI ACTGG 1822 1820 . . 1828 1833 + MstI* TGCGCA 1830 1830 . . 1829 1832 + Hin6I GCGC 1829 1831 . . 1829 1832 + GlaI GCGC 1830 1830 . . 1829 1832 + HhaI GCGC 1831 1829 . . 1836 1840 + TfiI GAWTC 1836 1839 . . 1836 1840 + HinfI GANTC 1836 1839 . . 1845 1849 + Tsp4CI* ACNGT 1847 1846 . . 1878 1881 - MnlI CCTC 1871 1870 . . 1882 1886 + TfiI GAWTC 1882 1885 . . 1882 1886 + HinfI GANTC 1882 1885 . . 1882 1892 + Hin4I GAYNNNNNVTC 1873 1868 1905 1900 1888 1892 + BsmAI GTCTC 1893 1897 . . 1890 1894 + DdeI CTNAG 1890 1893 . . 1890 1894 + BspCNI CTCAG 1903 1901 . . 1890 1894 + BseMII CTCAG 1904 1902 . . 1900 1904 + Hpy188I TCNGA 1902 1901 . . 1903 1913 + BplI GAGNNNNNCTC 1894 1889 1926 1921 1905 1910 + Hpy166II GTNNAC 1907 1907 . . 1905 1910 + HpaI GTTAAC 1907 1907 . . 1905 1910 + HindII GTYRAC 1907 1907 . . 1906 1909 + MseI TTAA 1906 1908 . . 1909 1912 + SetI ASST 1912 1908 . . 1910 1913 + MnlI CCTC 1920 1919 . . 1911 1916 + Ksp632I* CTCTTC 1917 1920 . . 1903 1913 - BplI GAGNNNNNCTC 1926 1921 1894 1889 1912 1916 - MboII GAAGA 1904 1903 . . 1920 1925 + SspI AATATT 1922 1922 . . 1943 1946 + TaqI TCGA 1943 1945 . . 1948 1951 + SetI ASST 1951 1947 . . 1948 1951 + CviJI RGCY 1949 1949 . . 1948 1951 + AluI AGCT 1949 1949 . . 1957 1962 + ClaI ATCGAT 1958 1960 . . 1958 1961 + TaqI TCGA 1958 1960 . . 1960 1963 + DpnI GATC 1961 1961 . . 1960 1963 + BstKTI GATC 1962 1960 . . 1960 1963 + MboI GATC 1959 1963 . . 1963 1966 + MaeI CTAG 1963 1965 . . 1970 1975 + Hpy178III* TCNNGA 1971 1973 . . 1986 1990 + TfiI GAWTC 1986 1989 . . 1986 1990 + HinfI GANTC 1986 1989 . . 1998 2001 + Csp6I GTAC 1998 2000 . . 1998 2001 + RsaI GTAC 1999 1999 . . 2003 2006 + TaqI TCGA 2003 2005 . . 1998 2010 - PsrI GAACNNNNNNTAC 2022 2017 1990 1985 2016 2021 + SspI AATATT 2018 2018 . . 2019 2024 + VspI ATTAAT 2020 2022 . . 2020 2023 + MseI TTAA 2020 2022 . . 2026 2030 - SfaNI GCATC 2016 2020 . . 2031 2035 + Hpy188I TCNGA 2033 2032 . . 2032 2036 + DdeI CTNAG 2032 2035 . . 2032 2036 - BspCNI CTCAG 2024 2022 . . 2032 2036 - BseMII CTCAG 2023 2021 . . 2040 2043 + CviRI* TGCA 2041 2041 . . 2043 2048 + TatI WGTACW 2043 2047 . . 2044 2047 + Csp6I GTAC 2044 2046 . . 2044 2047 + RsaI GTAC 2045 2045 . . 2067 2072 + TatI WGTACW 2067 2071 . . 2068 2071 + Csp6I GTAC 2068 2070 . . 2068 2071 + RsaI GTAC 2069 2069 . . 2075 2079 + TspDTI ATGAA 2090 2088 . . 2079 2082 + TspEI AATT 2078 2082 . . 2084 2087 + MnlI CCTC 2094 2093 . . 2086 2091 + XbaI TCTAGA 2086 2090 . . 2086 2091 + Hpy178III* TCNNGA 2087 2089 . . 2087 2090 + MaeI CTAG 2087 2089 . . 2095 2099 + Hpy188I TCNGA 2097 2096 . . 2101 2105 - BsrI ACTGG 2101 2099 . . 2100 2105 - BfiI ACTGGG 2095 2094 . . 2106 2109 + CviJI RGCY 2107 2107 . . 2110 2115 + MfeI CAATTG 2110 2114 . . 2111 2114 + TspEI AATT 2110 2114 . . 2133 2138 + ApoI RAATTY 2133 2137 . . 2134 2137 + TspEI AATT 2133 2137 . . 2139 2143 + Hpy188I TCNGA 2141 2140 . . 2140 2145 + Eco57MI CTGRAG 2161 2159 . . 2140 2145 + Eco57I CTGAAG 2161 2159 . . 2141 2152 + BdaI TGANNNNNNTCA 2130 2128 2164 2162 2142 2146 + MboII GAAGA 2154 2153 . . 2142 2151 + XmnI GAANNNNTTC 2146 2146 . . 2145 2150 + ApoI RAATTY 2145 2149 . . 2146 2149 + TspEI AATT 2145 2149 . . 2150 2155 + Hpy178III* TCNNGA 2151 2153 . . 2141 2152 - BdaI TGANNNNNNTCA 2164 2162 2130 2128 2165 2168 + TaqI TCGA 2165 2167 . . 2170 2173 + TspEI AATT 2169 2173 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 752 1423 # # AciI: # [CCGC] # 37 57 67 100 189 376 # 607 742 # # AclI: # [AACGTT] # 586 1589 # # AhaIII*: # [TTTAAA] # 225 266 367 560 757 # # AluI: # [AGCT] # 69 169 226 295 384 1032 # # AlwNI: # [CAGNNNCTG] # 395 469 1311 # # ApaLI: # [GTGCAC] # 1039 1136 # # ApoI: # [RAATTY] # 12 30 78 88 91 236 # 292 358 362 628 # # AsuI*: # [GGNCC] # 97 2078 # # AvaI: # [CYCGRG] # 141 549 1485 # # AvaII: # [GGWCC] # 97 2078 # # BamHI: # [GGATCC] # 300 1875 # # BarI: # [GAAGNNNNNNTAC] # 32 279 1864 # # BbvI: # [GCAGC] # 273 408 1494 # # BbvII*: # [GAAGAC] # 41 649 1485 # # BccI: # [CCATC] # 138 568 634 835 # # Bce83I*: # [CTTGAG] # 859 1316 # # BclI: # [TGATCA] # 119 846 1210 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 11 34 # 34 817 1279 # # BetI*: # [WCCGGW] # 21 2154 # # BfiI: # [ACTGGG] # 80 324 1771 # # BglI: # [GCCNNNNNGGC] # 1052 1123 # # BinI*: # [GGATC] # 13 93 202 797 1070 # # BisI: # [GCNGC] # 298 394 543 940 # # BlsI: # [GCNGC] # 298 393 544 940 # # BmeT110I: # [CYCGRG] # 142 549 1484 # # BmgT120I: # [GGNCC] # 98 2077 # # BplI: # [GAGNNNNNCTC] # 0 0 32 249 1894 # # Bpu10I: # [CCTNAGC] # 1069 1106 # # BsaAI: # [YACGTR] # 214 393 1568 # # BsaBI: # [GATNNNNATC] # 406 883 886 # # BsaXI: # [ACNNNNNCTCC] # 6 18 30 30 30 30 # 81 626 1324 # # BseBI: # [CCWGG] # 464 1711 # # BseGI: # [GGATG] # 39 118 136 192 237 249 # 509 695 # # BseMII: # [CTCAG] # 119 152 1904 # # BseRI: # [GAGGAG] # 78 830 1267 # # BseSI: # [GKGCMC] # 1035 1140 # # BsiYI*: # [CCNNNNNNNGG] # 93 272 292 1518 # # BslFI: # [GGGAC] # 92 397 1686 # # BsmAI: # [GTCTC] # 11 282 1882 # # BsmI: # [GAATGC] # 426 1749 # # BspCNI: # [CTCAG] # 121 151 1903 # # BsrI: # [ACTGG] # 56 74 279 280 404 1082 # # BssKI: # [CCNGG] # 462 1713 # # BstKTI: # [GATC] # 39 40 53 88 93 115 # 122 213 368 418 626 # # BtrI: # [CACGTC] # 39 2136 # # Cac8I: # [GCNNGC] # 124 167 936 948 # # CfrI: # [YGGCCR] # 540 1635 # # ClaI: # [ATCGAT] # 217 290 1668 # # Csp6I: # [GTAC] # 24 46 107 471 604 923 # # CviAII: # [CATG] # 114 120 241 259 279 290 # 329 543 # # CviJI: # [RGCY] # 9 9 10 14 16 19 # 23 35 47 47 50 67 # 68 69 143 146 158 312 # 461 472 # # CviRI*: # [TGCA] # 134 183 186 295 474 903 # # DdeI: # [CTNAG] # 107 142 143 154 155 348 # 497 629 # # DpnI: # [GATC] # 39 40 53 88 93 115 # 121 214 368 418 626 # # DsaI*: # [CCRYGG] # 234 521 597 823 # # EciI: # [GGCGGA] # 67 89 439 1580 # # Eco57I: # [CTGAAG] # 14 893 1268 # # Eco57MI: # [CTGRAG] # 14 356 537 1268 # # EcoRI: # [GAATTC] # 1069 1106 # # EcoRII: # [CCWGG] # 462 1713 # # FalI: # [AAGNNNNNCTT] # 0 0 32 370 1773 # # FatI: # [CATG] # 114 120 240 259 279 290 # 329 544 # # FnuDII*: # [CGCG] # 607 1568 # # FokI: # [GGATG] # 39 118 123 212 229 237 # 529 688 # # GlaI: # [GCGC] # 53 345 556 1221 # # GsuI: # [CTGGAG] # 370 1805 # # HaeIII: # [GGCC] # 430 542 1203 # # HgaI: # [GACGC] # 521 778 876 # # HgiAI*: # [GWGCWC] # 148 992 1035 # # HhaI: # [GCGC] # 54 344 556 1221 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 4 32 32 32 32 32 # 32 184 265 270 275 324 # 661 # # Hin4II*: # [CCTTC] # 143 396 1636 # # Hin6I: # [GCGC] # 52 346 556 1221 # # HindII: # [GTYRAC] # 45 118 123 171 230 268 # 444 776 # # HindIII: # [AAGCTT] # 1076 1099 # # HinfI: # [GANTC] # 46 104 189 363 646 827 # # HpaI: # [GTTAAC] # 45 268 289 353 444 776 # # HpaII: # [CCGG] # 22 2153 # # HphI: # [GGTGA] # 195 511 1469 # # Hpy166II: # [GTNNAC] # 7 12 45 46 65 118 # 171 230 268 286 444 483 # # Hpy178III*: # [TCNNGA] # 22 24 64 116 188 197 # 688 876 # # Hpy188I: # [TCNGA] # 34 44 64 131 225 240 # 300 390 747 # # Hpy99I: # [CGWCG] # 499 510 1166 # # Ksp632I*: # [CTCTTC] # 258 284 637 996 # # MaeI: # [CTAG] # 42 88 124 153 221 528 # 1019 # # MaeII: # [ACGT] # 20 23 38 171 173 354 # 477 919 # # MaeIII: # [GTNAC] # 8 94 128 377 399 541 # 628 # # MboI: # [GATC] # 39 40 53 88 93 115 # 119 216 368 418 626 # # MboII: # [GAAGA] # 4 11 21 21 37 46 # 46 85 105 142 243 248 # 250 268 304 344 # # MfeI: # [CAATTG] # 65 356 429 1325 # # MmeI: # [TCCRAC] # 109 143 328 352 577 666 # # MnlI: # [CCTC] # 9 24 24 32 49 81 # 94 102 117 171 174 198 # 207 260 279 354 # # MseI: # [TTAA] # 12 21 25 33 33 35 # 42 45 59 76 109 114 # 116 138 141 155 166 173 # 186 193 303 # # MslI: # [CAYNNNNRTG] # 239 1936 # # MstI*: # [TGCGCA] # 345 1830 # # MwoI: # [GCNNNNNNNGC] # 109 389 554 1123 # # NcoI: # [CCATGG] # 234 823 1118 # # NlaIII: # [CATG] # 114 120 244 259 279 290 # 329 540 # # NlaIV: # [GGNNCC] # 98 204 639 1234 # # NspBII*: # [CMGCKG] # 690 1485 # # NspI: # [RCATGY] # 863 1312 # # PflMI: # [CCANNNNNTGG] # 93 564 1518 # # PfoI: # [TCCNGGA] # 462 1713 # # PshAI: # [GACNNNNGTC] # 95 2080 # # PsiI: # [TTATAA] # 487 1688 # # PspXI: # [VCTCGAGB] # 141 2034 # # PsrI: # [GAACNNNNNNTAC] # 32 153 1990 # # PvuII: # [CAGCTG] # 690 1485 # # RsaI: # [GTAC] # 24 46 106 471 605 923 # # ScrFI: # [CCNGG] # 464 1711 # # SduI: # [GDGCHC] # 148 992 1035 # # SecI*: # [CCNNGG] # 48 168 178 234 381 521 # 645 # # SetI: # [ASST] # 8 15 16 18 20 23 # 25 39 59 65 69 69 # 82 85 86 93 94 124 # 130 135 141 224 260 295 # # SfaNI: # [GCATC] # 159 186 333 366 375 756 # # SmlI: # [CTYRAG] # 141 733 1301 # # SspI: # [AATATT] # 96 157 914 1008 # # StyI: # [CCWWGG] # 178 234 381 645 737 # # TaiI: # [ACGT] # 20 23 41 171 173 354 # 477 916 # # TaqI: # [TCGA] # 10 15 45 107 142 148 # 162 1546 # # TatI: # [WGTACW] # 24 108 2043 # # TauI: # [GCSGC] # 545 1630 # # TfiI: # [GAWTC] # 46 104 189 363 646 827 # # TseI: # [GCWGC] # 298 395 1482 # # TsoI: # [TARCCA] # 95 223 813 1044 # # Tsp45I: # [GTSAC] # 607 628 940 # # Tsp4CI*: # [ACNGT] # 20 28 122 191 232 280 # 328 404 570 # # TspDTI: # [ATGAA] # 16 52 85 122 124 125 # 264 405 470 512 # # TspEI: # [AATT] # 6 12 13 17 18 21 # 23 24 27 28 32 36 # 48 57 61 64 67 78 # 82 86 115 119 121 133 # 137 176 282 292 # # TspGWI: # [ACGGA] # 47 194 269 1665 # # VspI: # [ATTAAT] # 155 916 1104 # # XbaI: # [TCTAGA] # 89 2086 # # XcmI: # [CCANNNNNNNNNTGG] # 367 1808 # # XhoI: # [CTCGAG] # 141 2034 # # XhoII: # [RGATCY] # 93 207 792 1083 # # XmnI: # [GAANNNNTTC] # 29 357 494 1295 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2175 # Reported_sequences: 1 # Reported_hitcount: 538 #---------------------------------------