Restriction Fragments for TAF9/YMR236W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

TAF9/YMR236W on chromosome XIII from coordinates 742971 to 743444.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14770.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 5 9 + BceAI ACGGC 21 23 . . 7 12 + EciI GGCGGA 23 21 . . 8 11 - AciI CCGC 8 10 . . 11 14 - MnlI CCTC 4 3 . . 36 40 + DdeI CTNAG 36 39 . . 36 40 + BspCNI CTCAG 49 47 . . 36 40 + BseMII CTCAG 50 48 . . 38 44 + TspRI CASTGNN 44 35 . . 42 47 + SduI GDGCHC 46 42 . . 42 47 + HgiJII* GRGCYC 46 42 . . 43 46 + CviJI RGCY 44 44 . . 45 49 + DdeI CTNAG 45 48 . . 45 49 + BspCNI CTCAG 58 56 . . 45 49 + BseMII CTCAG 59 57 . . 52 56 + Hpy188I TCNGA 54 53 . . 61 65 + BmgT120I GGNCC 62 63 . . 61 65 + AvaII GGWCC 61 64 . . 61 65 + AsuI* GGNCC 61 64 . . 63 68 + Hpy178III* TCNNGA 64 66 . . 63 68 + BetI* WCCGGW 63 67 . . 63 68 + BspMII* TCCGGA 63 67 . . 64 67 + HpaII CCGG 64 66 . . 67 71 + HinfI GANTC 67 70 . . 67 71 - PleI GAGTC 61 62 . . 67 71 - MlyI GAGTC 61 61 . . 79 82 - MnlI CCTC 72 71 . . 89 100 + TstI CACNNNNNNTCC 80 75 112 107 89 94 + BsiI* CACGAG 89 93 . . 98 102 - TspGWI ACGGA 88 86 . . 107 110 + CviRI* TGCA 108 108 . . 108 112 + SfaNI GCATC 117 121 . . 117 120 + MnlI CCTC 127 126 . . 115 120 - BseRI GAGGAG 106 104 . . 121 125 + SfaNI GCATC 130 134 . . 126 130 + Tsp4CI* ACNGT 128 127 . . 130 133 + TaqI TCGA 130 132 . . 132 136 + TfiI GAWTC 132 135 . . 132 136 + HinfI GANTC 132 135 . . 121 132 - BcgI CGANNNNNNTGC 144 142 110 108 136 145 + MslI CAYNNNNRTG 140 140 . . 134 138 - TspDTI ATGAA 124 122 . . 147 151 + BmgT120I GGNCC 148 149 . . 147 151 + AvaII GGWCC 147 150 . . 147 151 + AsuI* GGNCC 147 150 . . 145 148 - MnlI CCTC 138 137 . . 154 157 + Csp6I GTAC 154 156 . . 154 157 + RsaI GTAC 155 155 . . 157 160 + AciI CCGC 157 159 . . 163 168 + MfeI CAATTG 163 167 . . 164 167 + TspEI AATT 163 167 . . 168 172 - BccI CCATC 162 163 . . 177 180 + CviRI* TGCA 178 178 . . 184 187 + SetI ASST 187 183 . . 186 191 + Hpy166II GTNNAC 188 188 . . 186 189 + Csp6I GTAC 186 188 . . 186 189 + RsaI GTAC 187 187 . . 191 202 + TstI CACNNNNNNTCC 182 177 214 209 194 204 + FalI AAGNNNNNCTT 185 180 217 212 197 201 + PleI GAGTC 205 206 . . 197 201 + MlyI GAGTC 206 206 . . 197 201 + HinfI GANTC 197 200 . . 202 207 + SmlI CTYRAG 202 206 . . 202 207 + AflII CTTAAG 202 206 . . 203 206 + MseI TTAA 203 205 . . 194 204 - FalI AAGNNNNNCTT 217 212 185 180 208 212 + HgaI GACGC 217 222 . . 209 214 + MluI ACGCGT 209 213 . . 209 214 + AflIII ACRYGT 209 213 . . 210 213 + FnuDII* CGCG 211 211 . . 217 222 + Hpy166II GTNNAC 219 219 . . 232 237 + Cac8I GCNNGC 234 234 . . 236 241 + BtsI GCAGTG 243 241 . . 237 243 + TspRI CASTGNN 243 234 . . 243 248 + ApoI RAATTY 243 247 . . 244 247 + TspEI AATT 243 247 . . 250 255 + NaeI GCCGGC 252 252 . . 250 255 + MroNI GCCGGC 250 254 . . 250 255 + Cac8I GCNNGC 252 252 . . 250 255 + Cfr10I RCCGGY 250 254 . . 251 254 + HpaII CCGG 251 253 . . 254 259 + BtsI GCAGTG 261 259 . . 255 261 + TspRI CASTGNN 261 252 . . 271 275 + MboII GAAGA 283 282 . . 271 276 + BbvII* GAAGAC 278 282 . . 279 283 + BceAI ACGGC 295 297 . . 281 284 + CviJI RGCY 282 282 . . 282 287 + Cac8I GCNNGC 284 284 . . 286 296 + MwoI GCNNNNNNNGC 292 289 . . 291 294 + CviRI* TGCA 292 292 . . 292 296 + BlsI GCNGC 294 293 . . 292 296 + BisI GCNGC 293 294 . . 292 296 + BbvI GCAGC 304 308 . . 292 296 + TseI GCWGC 292 295 . . 288 293 - BsrDI GCAATG 287 285 . . 311 314 + TspEI AATT 310 314 . . 314 317 + MseI TTAA 314 316 . . 317 320 + CviJI RGCY 318 318 . . 322 326 + BceAI ACGGC 338 340 . . 324 329 + NlaIV GGNNCC 326 326 . . 324 329 + HgiCI* GGYRCC 324 328 . . 327 330 + SetI ASST 330 326 . . 328 334 + SauI* CCTNAGG 329 332 . . 329 333 + DdeI CTNAG 329 332 . . 334 339 + SacI GAGCTC 338 334 . . 334 339 + Ecl136II GAGCTC 336 336 . . 334 339 + SduI GDGCHC 338 334 . . 334 339 + HgiJII* GRGCYC 338 334 . . 334 339 + HgiAI* GWGCWC 338 334 . . 335 338 + SetI ASST 338 334 . . 335 338 + CviJI RGCY 336 336 . . 335 338 + AluI AGCT 336 336 . . 339 342 + FatI CATG 338 342 . . 339 342 + NlaIII CATG 342 338 . . 339 342 + CviAII CATG 339 341 . . 342 346 + MaeIII GTNAC 341 346 . . 333 343 - BsaXI ACNNNNNCTCC 355 352 325 322 345 349 + Tsp4CI* ACNGT 347 346 . . 351 354 + SetI ASST 354 350 . . 351 354 + CviJI RGCY 352 352 . . 351 354 + AluI AGCT 352 352 . . 352 356 + BlsI GCNGC 354 353 . . 352 356 + BisI GCNGC 353 354 . . 352 356 + TseI GCWGC 352 355 . . 352 356 - BbvI GCAGC 339 343 . . 355 358 - AciI CCGC 355 357 . . 369 373 - Hin4II* CCTTC 363 362 . . 378 381 + SetI ASST 381 377 . . 379 382 + MnlI CCTC 389 388 . . 380 385 + SmlI CTYRAG 380 384 . . 380 385 - Bce83I* CTTGAG 365 363 . . 387 391 - BccI CCATC 381 382 . . 392 395 + Csp6I GTAC 392 394 . . 392 395 + RsaI GTAC 393 393 . . 393 398 + BsaAI YACGTR 395 395 . . 394 397 + TaiI ACGT 397 393 . . 394 397 + SetI ASST 397 393 . . 394 397 + MaeII ACGT 394 396 . . 407 410 + CviJI RGCY 408 408 . . 422 427 + SspI AATATT 424 424 . . 428 432 + BsmAI GTCTC 433 437 . . 438 442 - Hin4II* CCTTC 432 431 . . 446 452 + PpuMI RGGWCCY 447 450 . . 446 452 + DraII RGGNCCY 447 450 . . 446 450 + BslFI GGGAC 460 464 . . 446 451 + NlaIV GGNNCC 448 448 . . 447 451 + BmgT120I GGNCC 448 449 . . 447 451 + AvaII GGWCC 447 450 . . 447 451 + AsuI* GGNCC 447 450 . . 449 452 + SetI ASST 452 448 . . 451 456 + SmlI CTYRAG 451 455 . . 451 456 + Bce83I* CTTGAG 472 470 . . 455 461 + PpuMI RGGWCCY 456 459 . . 455 461 + DraII RGGNCCY 456 459 . . 456 461 + NlaIV GGNNCC 458 458 . . 456 460 + BmgT120I GGNCC 457 458 . . 456 460 + AvaII GGWCC 456 459 . . 456 460 + AsuI* GGNCC 456 459 . . 454 457 - MnlI CCTC 447 446 . . 457 467 + Hin4I GAYNNNNNVTC 448 443 . . 457 467 + Eam1105I GACNNNNNGTC 462 461 . . #--------------------------------------- # # # AciI: # [CCGC] # 8 119 149 198 # # AflII: # [CTTAAG] # 202 272 # # AflIII: # [ACRYGT] # 209 265 # # AluI: # [AGCT] # 16 122 336 # # ApoI: # [RAATTY] # 231 243 # # AsuI*: # [GGNCC] # 9 18 61 86 300 # # AvaII: # [GGWCC] # 9 18 61 86 300 # # BbvI: # [GCAGC] # 35 135 304 # # BbvII*: # [GAAGAC] # 196 278 # # BccI: # [CCATC] # 93 162 219 # # Bce83I*: # [CTTGAG] # 2 107 365 # # BceAI: # [ACGGC] # 21 43 136 274 # # BcgI: # [CGANNNNNNTGC] # 34 110 330 # # BetI*: # [WCCGGW] # 63 411 # # BisI: # [GCNGC] # 60 121 293 # # BlsI: # [GCNGC] # 60 120 294 # # BmgT120I: # [GGNCC] # 9 17 62 86 300 # # BsaAI: # [YACGTR] # 79 395 # # BsaXI: # [ACNNNNNCTCC] # 30 119 325 # # BseMII: # [CTCAG] # 9 50 415 # # BseRI: # [GAGGAG] # 106 368 # # BsiI*: # [CACGAG] # 89 385 # # BslFI: # [GGGAC] # 14 460 # # BsmAI: # [GTCTC] # 41 433 # # BspCNI: # [CTCAG] # 9 49 416 # # BspMII*: # [TCCGGA] # 63 411 # # BsrDI: # [GCAATG] # 187 287 # # BtsI: # [GCAGTG] # 18 213 243 # # Cac8I: # [GCNNGC] # 18 32 190 234 # # Cfr10I: # [RCCGGY] # 224 250 # # Csp6I: # [GTAC] # 32 82 154 206 # # CviAII: # [CATG] # 135 339 # # CviJI: # [RGCY] # 16 18 36 44 56 66 # 238 # # CviRI*: # [TGCA] # 70 108 114 182 # # DdeI: # [CTNAG] # 9 36 145 284 # # DraII: # [RGGNCCY] # 9 18 447 # # Eam1105I: # [GACNNNNNGTC] # 12 462 # # EciI: # [GGCGGA] # 23 451 # # Ecl136II: # [GAGCTC] # 138 336 # # FalI: # [AAGNNNNNCTT] # 0 0 32 185 257 # # FatI: # [CATG] # 136 338 # # FnuDII*: # [CGCG] # 211 263 # # HgaI: # [GACGC] # 217 257 # # HgiAI*: # [GWGCWC] # 136 338 # # HgiCI*: # [GGYRCC] # 150 324 # # HgiJII*: # [GRGCYC] # 46 136 292 # # Hin4I: # [GAYNNNNNVTC] # 26 448 # # Hin4II*: # [CCTTC] # 42 69 363 # # HinfI: # [GANTC] # 65 65 67 277 # # HpaII: # [CCGG] # 64 187 223 # # Hpy166II: # [GTNNAC] # 31 188 255 # # Hpy178III*: # [TCNNGA] # 64 410 # # Hpy188I: # [TCNGA] # 54 420 # # MaeII: # [ACGT] # 80 394 # # MaeIII: # [GTNAC] # 133 341 # # MboII: # [GAAGA] # 191 283 # # MfeI: # [CAATTG] # 163 311 # # MluI: # [ACGCGT] # 209 265 # # MlyI: # [GAGTC] # 61 145 268 # # MnlI: # [CCTC] # 4 11 27 55 58 68 # 251 # # MroNI: # [GCCGGC] # 224 250 # # MseI: # [TTAA] # 111 160 203 # # MslI: # [CAYNNNNRTG] # 140 334 # # MwoI: # [GCNNNNNNNGC] # 182 292 # # NaeI: # [GCCGGC] # 222 252 # # NlaIII: # [CATG] # 132 342 # # NlaIV: # [GGNNCC] # 10 16 122 326 # # PleI: # [GAGTC] # 61 144 269 # # PpuMI: # [RGGWCCY] # 9 18 447 # # RsaI: # [GTAC] # 32 81 155 206 # # SacI: # [GAGCTC] # 136 338 # # SauI*: # [CCTNAGG] # 145 329 # # SduI: # [GDGCHC] # 46 136 292 # # SetI: # [ASST] # 8 16 16 22 27 55 # 143 187 # # SfaNI: # [GCATC] # 13 117 344 # # SmlI: # [CTYRAG] # 23 71 178 202 # # SspI: # [AATATT] # 50 424 # # TaiI: # [ACGT] # 77 397 # # TaqI: # [TCGA] # 130 344 # # TfiI: # [GAWTC] # 132 342 # # TseI: # [GCWGC] # 60 122 292 # # Tsp4CI*: # [ACNGT] # 127 128 219 # # TspDTI: # [ATGAA] # 16 108 350 # # TspEI: # [AATT] # 67 80 163 164 # # TspGWI: # [ACGGA] # 88 386 # # TspRI: # [CASTGNN] # 18 44 199 213 # # TstI: # [CACNNNNNNTCC] # 32 32 70 80 260 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 474 # Reported_sequences: 1 # Reported_hitcount: 161 #---------------------------------------