Restriction Fragments for PFK2/YMR205C



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The currently selected gene/sequence is :

PFK2/YMR205C on chromosome XIII from coordinates 674766 to 671887.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.11759.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + Tsp4CI* ACNGT 6 5 . . 7 11 + MaeIII GTNAC 6 11 . . 29 32 + Csp6I GTAC 29 31 . . 29 32 + RsaI GTAC 30 30 . . 41 44 + Csp6I GTAC 41 43 . . 41 44 + RsaI GTAC 42 42 . . 43 47 + Tsp4CI* ACNGT 45 44 . . 46 50 + Tsp45I GTSAC 45 50 . . 46 50 + MaeIII GTNAC 45 50 . . 48 54 + TspRI CASTGNN 54 45 . . 51 54 + CviRI* TGCA 52 52 . . 48 53 - BtsI GCAGTG 47 45 . . 58 62 - TspGWI ACGGA 48 46 . . 69 74 + PsiI TTATAA 71 71 . . 75 78 + SetI ASST 78 74 . . 75 78 + CviJI RGCY 76 76 . . 75 78 + AluI AGCT 76 76 . . 76 80 + BlsI GCNGC 78 77 . . 76 80 + BisI GCNGC 77 78 . . 76 80 + TseI GCWGC 76 79 . . 76 80 - BbvI GCAGC 63 67 . . 117 120 + AciI CCGC 117 119 . . 117 122 + BsrBI CCGCTC 119 119 . . 119 125 + SapI GCTCTTC 126 129 . . 120 125 + Ksp632I* CTCTTC 126 129 . . 121 125 - MboII GAAGA 113 112 . . 126 131 + Hpy178III* TCNNGA 127 129 . . 130 140 + Hin4I GAYNNNNNVTC 121 116 153 148 125 130 - Eco57MI CTGRAG 110 108 . . 125 130 - GsuI CTGGAG 110 108 . . 131 135 + TspDTI ATGAA 146 144 . . 157 161 + TfiI GAWTC 157 160 . . 157 161 + HinfI GANTC 157 160 . . 157 167 + Hin4I GAYNNNNNVTC 148 143 180 175 161 165 + BccI CCATC 169 170 . . 171 175 + MboII GAAGA 183 182 . . 173 178 + XhoII RGATCY 173 177 . . 174 177 + DpnI GATC 175 175 . . 174 177 + BstKTI GATC 176 174 . . 174 177 + MboI GATC 173 177 . . 177 181 + Hin4II* CCTTC 187 186 . . 174 178 - BinI* GGATC 168 169 . . 183 186 + TaiI ACGT 186 182 . . 183 186 + SetI ASST 186 182 . . 183 186 + MaeII ACGT 183 185 . . 186 191 + Hpy178III* TCNNGA 187 189 . . 191 195 + TspDTI ATGAA 206 204 . . 216 219 + CviJI RGCY 217 217 . . 214 217 - MnlI CCTC 207 206 . . 225 229 - Hin4II* CCTTC 219 218 . . 230 233 + TspEI AATT 229 233 . . 236 240 + Tsp4CI* ACNGT 238 237 . . 249 253 + HinfI GANTC 249 252 . . 251 256 + SmlI CTYRAG 251 255 . . 252 257 + Hpy178III* TCNNGA 253 255 . . 249 253 - PleI GAGTC 243 244 . . 249 253 - MlyI GAGTC 243 243 . . 256 266 + Hin4I GAYNNNNNVTC 247 242 279 274 251 256 - Bce83I* CTTGAG 236 234 . . 257 261 + BsrI ACTGG 262 260 . . 258 263 + Eco57MI CTGRAG 279 277 . . 258 263 + GsuI CTGGAG 279 277 . . 263 266 + DpnI GATC 264 264 . . 263 266 + BstKTI GATC 265 263 . . 263 266 + MboI GATC 262 266 . . 256 266 - Hin4I GAYNNNNNVTC 279 274 247 242 267 272 + NspI RCATGY 271 267 . . 268 271 + FatI CATG 267 271 . . 268 271 + NlaIII CATG 271 267 . . 268 271 + CviAII CATG 268 270 . . 272 286 + XcmI CCANNNNNNNNNTGG 279 278 . . 273 278 + TaqII CACCCA 289 287 . . 276 286 + BsiYI* CCNNNNNNNGG 282 279 . . 276 286 + PflMI CCANNNNNTGG 282 279 . . 281 286 + StyI CCWWGG 281 285 . . 281 286 + SecI* CCNNGG 281 285 . . 290 293 + MseI TTAA 290 292 . . 298 303 + MmeI TCCRAC 323 321 . . 298 302 + Hpy188I TCNGA 300 299 . . 331 341 + MwoI GCNNNNNNNGC 337 334 . . 334 338 + TspDTI ATGAA 349 347 . . 343 346 + MnlI CCTC 353 352 . . 343 353 + EcoNI CCTNNNNNAGG 347 348 . . 343 353 + BsiYI* CCNNNNNNNGG 349 346 . . 344 349 + Ksp632I* CTCTTC 350 353 . . 341 346 - BseRI GAGGAG 332 330 . . 345 349 - MboII GAAGA 337 336 . . 352 355 + CviJI RGCY 353 353 . . 364 373 + XmnI GAANNNNTTC 368 368 . . 377 380 + CviRI* TGCA 378 378 . . 383 388 + TatI WGTACW 383 387 . . 383 388 + Bsp1407I TGTACA 383 387 . . 384 389 + Hpy166II GTNNAC 386 386 . . 384 387 + Csp6I GTAC 384 386 . . 384 387 + RsaI GTAC 385 385 . . 393 398 + NlaIV GGNNCC 395 395 . . 393 397 + BmgT120I GGNCC 394 395 . . 393 397 + AvaII GGWCC 393 396 . . 393 397 + AsuI* GGNCC 393 396 . . 402 405 + SetI ASST 405 401 . . 404 408 + MaeIII GTNAC 403 408 . . 406 411 + AclI AACGTT 407 409 . . 407 410 + TaiI ACGT 410 406 . . 407 410 + SetI ASST 410 406 . . 407 410 + MaeII ACGT 407 409 . . 418 422 + MaeIII GTNAC 417 422 . . 428 432 + DdeI CTNAG 428 431 . . 449 452 + CviJI RGCY 450 450 . . 466 476 + MwoI GCNNNNNNNGC 472 469 . . 473 478 + HindIII AAGCTT 473 477 . . 474 477 + SetI ASST 477 473 . . 474 477 + CviJI RGCY 475 475 . . 474 477 + AluI AGCT 475 475 . . 482 486 + DdeI CTNAG 482 485 . . 484 488 + PleI GAGTC 492 493 . . 484 488 + MlyI GAGTC 493 493 . . 484 488 + HinfI GANTC 484 487 . . 479 484 - EcoP15I CAGCAG 451 453 . . 482 486 - BspCNI CTCAG 474 472 . . 482 486 - BseMII CTCAG 473 471 . . 497 500 + MnlI CCTC 507 506 . . 505 510 + Hpy166II GTNNAC 507 507 . . 516 522 + TspRI CASTGNN 522 513 . . 525 528 + CviJI RGCY 526 526 . . 530 534 + Tsp4CI* ACNGT 532 531 . . 535 539 + TspDTI ATGAA 550 548 . . 550 553 + SetI ASST 553 549 . . 556 560 + BccI CCATC 564 565 . . 569 572 + CviJI RGCY 570 570 . . 580 585 + StuI AGGCCT 582 582 . . 581 584 + CviJI RGCY 582 582 . . 581 584 + HaeIII GGCC 582 582 . . 583 586 + MnlI CCTC 593 592 . . 593 598 + MfeI CAATTG 593 597 . . 594 597 + TspEI AATT 593 597 . . 602 607 + Hpy178III* TCNNGA 603 605 . . 602 607 + BspHI TCATGA 602 606 . . 598 602 - BceAI ACGGC 583 585 . . 603 606 + FatI CATG 602 606 . . 603 606 + NlaIII CATG 606 602 . . 603 606 + CviAII CATG 603 605 . . 610 615 + BetI* WCCGGW 610 614 . . 611 614 + HpaII CCGG 611 613 . . 616 620 + HphI GGTGA 628 627 . . 619 623 - SfaNI GCATC 609 613 . . 625 629 + BssKI CCNGG 624 629 . . 625 629 + BseBI CCWGG 626 627 . . 625 629 + ScrFI CCNGG 626 627 . . 625 629 + EcoRII CCWGG 624 629 . . 627 630 + SetI ASST 630 626 . . 623 628 - Eco57MI CTGRAG 608 606 . . 623 628 - GsuI CTGGAG 608 606 . . 631 635 + TspDTI ATGAA 646 644 . . 640 645 + AclI AACGTT 641 643 . . 641 644 + TaiI ACGT 644 640 . . 641 644 + SetI ASST 644 640 . . 641 644 + MaeII ACGT 641 643 . . 648 651 + CviJI RGCY 649 649 . . 650 654 + BccI CCATC 658 659 . . 662 665 + AciI CCGC 662 664 . . 668 672 - MboII GAAGA 660 659 . . 675 678 + SetI ASST 678 674 . . 696 699 + FatI CATG 695 699 . . 696 699 + NlaIII CATG 699 695 . . 696 699 + CviAII CATG 696 698 . . 702 705 + SetI ASST 705 701 . . 700 704 - Hin4II* CCTTC 694 693 . . 707 711 + TspDTI ATGAA 722 720 . . 711 714 + SetI ASST 714 710 . . 709 713 - Hin4II* CCTTC 703 702 . . 727 731 + BmgT120I GGNCC 728 729 . . 727 731 + AvaII GGWCC 727 730 . . 727 731 + AsuI* GGNCC 727 730 . . 729 734 + Hpy178III* TCNNGA 730 732 . . 745 750 + EcoRI GAATTC 745 749 . . 745 750 + ApoI RAATTY 745 749 . . 746 749 + TspEI AATT 745 749 . . 751 757 + TspRI CASTGNN 757 748 . . 752 756 + BsrI ACTGG 757 755 . . 752 757 + BfiI ACTGGG 762 761 . . 757 761 + MboII GAAGA 769 768 . . 757 762 + BbvII* GAAGAC 764 768 . . 760 765 + ZraI GACGTC 762 762 . . 760 765 + AcyI GRCGYC 761 763 . . 760 765 + AatII GACGTC 764 760 . . 761 764 + TaiI ACGT 764 760 . . 761 764 + SetI ASST 764 760 . . 761 764 + MaeII ACGT 761 763 . . 765 770 + DsaI* CCRYGG 765 769 . . 765 770 + SecI* CCNNGG 765 769 . . 764 768 - TspGWI ACGGA 754 752 . . 779 784 + Eco57MI CTGRAG 800 798 . . 779 784 + Eco57I CTGAAG 800 798 . . 776 781 - EcoP15I CAGCAG 748 750 . . 783 786 + SetI ASST 786 782 . . 781 785 - Hin4II* CCTTC 775 774 . . 787 792 + KpnI GGTACC 791 787 . . 787 792 + NlaIV GGNNCC 789 789 . . 787 792 + HgiCI* GGYRCC 787 791 . . 787 792 + Acc65I GGTACC 787 791 . . 788 791 + Csp6I GTAC 788 790 . . 788 791 + RsaI GTAC 789 789 . . 800 803 + Csp6I GTAC 800 802 . . 800 803 + RsaI GTAC 801 801 . . 807 817 + BsiYI* CCNNNNNNNGG 813 810 . . 817 822 + EcoRI GAATTC 817 821 . . 817 822 + ApoI RAATTY 817 821 . . 818 821 + TspEI AATT 817 821 . . 821 826 + Hpy178III* TCNNGA 822 824 . . 828 831 + Hin6I GCGC 828 830 . . 828 831 + GlaI GCGC 829 829 . . 828 831 + HhaI GCGC 830 828 . . 829 832 + FnuDII* CGCG 830 830 . . 834 837 + SetI ASST 837 833 . . 832 836 - Hin4II* CCTTC 826 825 . . 847 852 + NlaIV GGNNCC 849 849 . . 847 852 + HgiCI* GGYRCC 847 851 . . 848 853 + SduI GDGCHC 852 848 . . 848 853 + BseSI GKGCMC 852 848 . . 845 850 - TaqII CACCCA 835 833 . . 867 870 + CviJI RGCY 868 868 . . 867 870 + HaeIII GGCC 868 868 . . 865 868 - MnlI CCTC 858 857 . . 868 873 + Cfr10I RCCGGY 868 872 . . 869 872 + HpaII CCGG 869 871 . . 875 878 + TaqI TCGA 875 877 . . 877 881 - SfaNI GCATC 867 871 . . 885 888 + DpnI GATC 886 886 . . 885 888 + BstKTI GATC 887 885 . . 885 888 + MboI GATC 884 888 . . 898 902 + HphI GGTGA 910 909 . . 899 903 + Tsp45I GTSAC 898 903 . . 899 903 + MaeIII GTNAC 898 903 . . 902 906 + Tsp4CI* ACNGT 904 903 . . 913 917 + BsrI ACTGG 918 916 . . 922 925 + DpnI GATC 923 923 . . 922 925 + BstKTI GATC 924 922 . . 922 925 + MboI GATC 921 925 . . 932 935 + DpnI GATC 933 933 . . 932 935 + BstKTI GATC 934 932 . . 932 935 + MboI GATC 931 935 . . 934 938 + Hpy188I TCNGA 936 935 . . 941 944 + CviJI RGCY 942 942 . . 941 944 + HaeIII GGCC 942 942 . . 943 947 + Hin4II* CCTTC 953 952 . . 951 954 + DpnI GATC 952 952 . . 951 954 + BstKTI GATC 953 951 . . 951 954 + MboI GATC 950 954 . . 953 956 + TaqI TCGA 953 955 . . 955 958 - MnlI CCTC 948 947 . . 959 962 + TspEI AATT 958 962 . . 977 982 + ApoI RAATTY 977 981 . . 978 981 + TspEI AATT 977 981 . . 982 987 + MmeI TCCRAC 1007 1005 . . 1003 1007 + TspDTI ATGAA 1018 1016 . . 1015 1020 + SspI AATATT 1017 1017 . . 1025 1028 + Csp6I GTAC 1025 1027 . . 1025 1028 + RsaI GTAC 1026 1026 . . 1022 1025 - AciI CCGC 1022 1024 . . 1027 1031 + Tsp4CI* ACNGT 1029 1028 . . 1025 1037 - PsrI GAACNNNNNNTAC 1049 1044 1017 1012 1053 1058 + Hpy166II GTNNAC 1055 1055 . . 1058 1063 + DsaI* CCRYGG 1058 1062 . . 1058 1063 + SecI* CCNNGG 1058 1062 . . 1060 1064 + TspGWI ACGGA 1075 1073 . . 1062 1066 + FokI GGATG 1075 1079 . . 1062 1066 + BseGI GGATG 1068 1066 . . 1063 1067 - SfaNI GCATC 1053 1057 . . 1078 1088 + MwoI GCNNNNNNNGC 1084 1081 . . 1088 1093 + StyI CCWWGG 1088 1092 . . 1088 1093 + SecI* CCNNGG 1088 1092 . . 1095 1103 + AlwNI CAGNNNCTG 1100 1097 . . 1097 1101 + TfiI GAWTC 1097 1100 . . 1097 1101 + HinfI GANTC 1097 1100 . . 1107 1110 + CviJI RGCY 1108 1108 . . 1107 1110 + HaeIII GGCC 1108 1108 . . 1109 1113 + BccI CCATC 1117 1118 . . 1111 1116 + ClaI ATCGAT 1112 1114 . . 1112 1115 + TaqI TCGA 1112 1114 . . 1118 1121 + TaiI ACGT 1121 1117 . . 1118 1121 + SetI ASST 1121 1117 . . 1118 1121 + MaeII ACGT 1118 1120 . . 1125 1128 + CviJI RGCY 1126 1126 . . 1128 1134 + TspRI CASTGNN 1134 1125 . . 1128 1133 - BtsI GCAGTG 1127 1125 . . 1143 1148 + SmlI CTYRAG 1143 1147 . . 1144 1149 + Hpy178III* TCNNGA 1145 1147 . . 1143 1148 - Bce83I* CTTGAG 1128 1126 . . 1149 1152 + SetI ASST 1152 1148 . . 1149 1152 + CviJI RGCY 1150 1150 . . 1149 1152 + AluI AGCT 1150 1150 . . 1175 1188 + AjuI GAANNNNNNNTTGG 1167 1162 1199 1194 1178 1182 + Tsp4CI* ACNGT 1180 1179 . . 1186 1191 - TsoI TARCCA 1176 1174 . . 1191 1194 + SetI ASST 1194 1190 . . 1191 1194 + CviJI RGCY 1192 1192 . . 1191 1194 + AluI AGCT 1192 1192 . . 1192 1202 + MwoI GCNNNNNNNGC 1198 1195 . . 1200 1203 + SetI ASST 1203 1199 . . 1200 1203 + CviJI RGCY 1201 1201 . . 1200 1203 + AluI AGCT 1201 1201 . . 1201 1211 + MwoI GCNNNNNNNGC 1207 1204 . . 1210 1220 + MwoI GCNNNNNNNGC 1216 1213 . . 1217 1222 + NspBII* CMGCKG 1219 1219 . . 1205 1217 - BarI GAAGNNNNNNTAC 1229 1224 1197 1192 1217 1220 + AciI CCGC 1217 1219 . . 1236 1241 + Hpy178III* TCNNGA 1237 1239 . . 1244 1247 + CviJI RGCY 1245 1245 . . 1245 1250 + Cac8I GCNNGC 1247 1247 . . 1248 1251 + CviJI RGCY 1249 1249 . . 1256 1266 + BsiYI* CCNNNNNNNGG 1262 1259 . . 1256 1266 + PflMI CCANNNNNTGG 1262 1259 . . 1270 1273 + DpnI GATC 1271 1271 . . 1270 1273 + BstKTI GATC 1272 1270 . . 1270 1273 + MboI GATC 1269 1273 . . 1280 1284 + Tsp45I GTSAC 1279 1284 . . 1280 1284 + MaeIII GTNAC 1279 1284 . . 1282 1292 + Hin4I GAYNNNNNVTC 1273 1268 1305 1300 1282 1290 + Tth111I GACNNNGTC 1285 1286 . . 1283 1293 + BsaXI ACNNNNNCTCC 1273 1270 1303 1300 1288 1292 + BsmAI GTCTC 1293 1297 . . 1301 1304 + DpnI GATC 1302 1302 . . 1301 1304 + BstKTI GATC 1303 1301 . . 1301 1304 + MboI GATC 1300 1304 . . 1319 1330 - CspCI CAANNNNNGTGG 1343 1341 1308 1306 1335 1338 + CviRI* TGCA 1336 1336 . . 1336 1346 + MwoI GCNNNNNNNGC 1342 1339 . . 1336 1346 + BstAPI GCANNNNNTGC 1342 1339 . . 1341 1344 + SetI ASST 1344 1340 . . 1339 1343 - Hin4II* CCTTC 1333 1332 . . 1345 1355 + MwoI GCNNNNNNNGC 1351 1348 . . 1351 1355 + BlsI GCNGC 1353 1352 . . 1351 1355 + BisI GCNGC 1352 1353 . . 1351 1355 + TseI GCWGC 1351 1354 . . 1351 1355 - BbvI GCAGC 1338 1342 . . 1352 1357 - EcoP15I CAGCAG 1324 1326 . . 1368 1371 + TspEI AATT 1367 1371 . . 1380 1384 + Hpy99I CGWCG 1384 1379 . . 1381 1386 + ZraI GACGTC 1383 1383 . . 1381 1386 + AcyI GRCGYC 1382 1384 . . 1381 1386 + AatII GACGTC 1385 1381 . . 1382 1385 + TaiI ACGT 1385 1381 . . 1382 1385 + SetI ASST 1385 1381 . . 1382 1385 + MaeII ACGT 1382 1384 . . 1384 1389 + Hpy166II GTNNAC 1386 1386 . . 1396 1399 + MaeI CTAG 1396 1398 . . 1399 1404 + Hpy166II GTNNAC 1401 1401 . . 1399 1404 + HindII GTYRAC 1401 1401 . . 1410 1413 + SetI ASST 1413 1409 . . 1416 1421 - BciVI GTATCC 1410 1409 . . 1425 1428 + TspEI AATT 1424 1428 . . 1432 1435 + SetI ASST 1435 1431 . . 1433 1439 + SauI* CCTNAGG 1434 1437 . . 1434 1438 + DdeI CTNAG 1434 1437 . . 1437 1440 + SetI ASST 1440 1436 . . 1439 1443 + Tsp45I GTSAC 1438 1443 . . 1439 1443 + MaeIII GTNAC 1438 1443 . . 1442 1445 + TaiI ACGT 1445 1441 . . 1442 1445 + SetI ASST 1445 1441 . . 1442 1445 + MaeII ACGT 1442 1444 . . 1452 1455 + SetI ASST 1455 1451 . . 1451 1454 - MnlI CCTC 1444 1443 . . 1457 1460 + Csp6I GTAC 1457 1459 . . 1457 1460 + RsaI GTAC 1458 1458 . . 1460 1465 - EcoP15I CAGCAG 1432 1434 . . 1456 1466 - BaeI ACNNNNGTAYC 1481 1476 1448 1443 1472 1482 + BaeI ACNNNNGTAYC 1461 1456 1494 1489 1473 1478 + McrI* CGRYCG 1476 1474 . . 1475 1479 + Tsp4CI* ACNGT 1477 1476 . . 1476 1486 + BsiYI* CCNNNNNNNGG 1482 1479 . . 1485 1488 + CviJI RGCY 1486 1486 . . 1484 1489 - TsoI TARCCA 1474 1472 . . 1497 1500 + SetI ASST 1500 1496 . . 1500 1505 + Hpy178III* TCNNGA 1501 1503 . . 1501 1506 + SmlI CTYRAG 1501 1505 . . 1501 1506 + Bce83I* CTTGAG 1522 1520 . . 1506 1509 + CviJI RGCY 1507 1507 . . 1506 1509 + HaeIII GGCC 1507 1507 . . 1504 1507 - MnlI CCTC 1497 1496 . . 1507 1517 + MwoI GCNNNNNNNGC 1513 1510 . . 1511 1514 + MseI TTAA 1511 1513 . . 1507 1511 - BceAI ACGGC 1492 1494 . . 1516 1520 - BceAI ACGGC 1501 1503 . . 1525 1529 + TfiI GAWTC 1525 1528 . . 1525 1529 + HinfI GANTC 1525 1528 . . 1533 1538 + Hpy178III* TCNNGA 1534 1536 . . 1537 1547 + Hin4I GAYNNNNNVTC 1528 1523 1560 1555 1532 1537 - Eco57MI CTGRAG 1517 1515 . . 1532 1537 - GsuI CTGGAG 1517 1515 . . 1543 1547 + BccI CCATC 1551 1552 . . 1537 1547 - Hin4I GAYNNNNNVTC 1560 1555 1528 1523 1546 1550 - HphI GGTGA 1538 1537 . . 1561 1566 + Hpy166II GTNNAC 1563 1563 . . 1561 1566 + HpaI GTTAAC 1563 1563 . . 1561 1566 + HindII GTYRAC 1563 1563 . . 1562 1565 + MseI TTAA 1562 1564 . . 1575 1578 + TspEI AATT 1574 1578 . . 1583 1588 + Hpy166II GTNNAC 1585 1585 . . 1590 1595 + VspI ATTAAT 1591 1593 . . 1591 1594 + MseI TTAA 1591 1593 . . 1597 1601 + TfiI GAWTC 1597 1600 . . 1597 1601 + HinfI GANTC 1597 1600 . . 1600 1604 - TspGWI ACGGA 1590 1588 . . 1608 1613 + Hpy166II GTNNAC 1610 1610 . . 1608 1613 + HindII GTYRAC 1610 1610 . . 1623 1626 + CviRI* TGCA 1624 1624 . . 1627 1636 + XmnI GAANNNNTTC 1631 1631 . . 1629 1632 + CviJI RGCY 1630 1630 . . 1630 1640 + MwoI GCNNNNNNNGC 1636 1633 . . 1638 1641 + SetI ASST 1641 1637 . . 1638 1641 + CviJI RGCY 1639 1639 . . 1638 1641 + AluI AGCT 1639 1639 . . 1640 1644 + DdeI CTNAG 1640 1643 . . 1639 1645 - Bpu10I CCTNAGC 1640 1643 . . 1649 1654 + Hpy178III* TCNNGA 1650 1652 . . 1656 1659 + SetI ASST 1659 1655 . . 1656 1659 + CviJI RGCY 1657 1657 . . 1656 1659 + AluI AGCT 1657 1657 . . 1666 1669 + MseI TTAA 1666 1668 . . 1674 1680 + TspRI CASTGNN 1680 1671 . . 1670 1674 - BsmAI GTCTC 1664 1668 . . 1676 1680 + DdeI CTNAG 1676 1679 . . 1676 1680 - BspCNI CTCAG 1668 1666 . . 1676 1680 - BseMII CTCAG 1667 1665 . . 1681 1685 - TspDTI ATGAA 1671 1669 . . 1692 1697 + AhaIII* TTTAAA 1694 1694 . . 1693 1696 + MseI TTAA 1693 1695 . . 1699 1702 + TspEI AATT 1698 1702 . . 1704 1707 + FatI CATG 1703 1707 . . 1704 1707 + NlaIII CATG 1707 1703 . . 1704 1707 + CviAII CATG 1704 1706 . . 1702 1706 - TspDTI ATGAA 1692 1690 . . 1707 1710 + CviJI RGCY 1708 1708 . . 1706 1711 - TsoI TARCCA 1696 1694 . . 1718 1723 - EcoP15I CAGCAG 1690 1692 . . 1732 1744 + PsrI GAACNNNNNNTAC 1724 1719 1756 1751 1739 1742 + SetI ASST 1742 1738 . . 1739 1742 + CviJI RGCY 1740 1740 . . 1739 1742 + AluI AGCT 1740 1740 . . 1759 1764 + Eco57MI CTGRAG 1780 1778 . . 1755 1759 - BsmAI GTCTC 1749 1753 . . 1759 1764 + Eco57I CTGAAG 1780 1778 . . 1761 1765 + MboII GAAGA 1773 1772 . . 1775 1778 + MseI TTAA 1775 1777 . . 1784 1789 + SduI GDGCHC 1788 1784 . . 1784 1789 + HgiAI* GWGCWC 1788 1784 . . 1789 1799 + BsiYI* CCNNNNNNNGG 1795 1792 . . 1789 1799 + PflMI CCANNNNNTGG 1795 1792 . . 1790 1795 + PvuII CAGCTG 1792 1792 . . 1790 1795 + NspBII* CMGCKG 1792 1792 . . 1791 1794 + SetI ASST 1794 1790 . . 1791 1794 + CviJI RGCY 1792 1792 . . 1791 1794 + AluI AGCT 1792 1792 . . 1787 1792 - Eco57MI CTGRAG 1772 1770 . . 1787 1792 - GsuI CTGGAG 1772 1770 . . 1813 1818 + Hpy166II GTNNAC 1815 1815 . . 1813 1818 + AccI GTMKAC 1814 1816 . . 1810 1814 - BceAI ACGGC 1795 1797 . . 1819 1822 + TaqI TCGA 1819 1821 . . 1824 1827 + CviJI RGCY 1825 1825 . . 1821 1825 - BccI CCATC 1815 1816 . . 1823 1828 - TsoI TARCCA 1813 1811 . . 1832 1836 + Tsp4CI* ACNGT 1834 1833 . . 1842 1847 + StyI CCWWGG 1842 1846 . . 1842 1847 + SecI* CCNNGG 1842 1846 . . 1839 1843 - BslFI GGGAC 1824 1828 . . 1845 1848 + SetI ASST 1848 1844 . . 1847 1851 + Tsp45I GTSAC 1846 1851 . . 1847 1851 + MaeIII GTNAC 1846 1851 . . 1894 1903 + MslI CAYNNNNRTG 1898 1898 . . 1894 1897 + FatI CATG 1893 1897 . . 1894 1897 + NlaIII CATG 1897 1893 . . 1894 1897 + CviAII CATG 1894 1896 . . 1895 1899 + TspDTI ATGAA 1910 1908 . . 1897 1906 + XmnI GAANNNNTTC 1901 1901 . . 1916 1920 + BsrI ACTGG 1921 1919 . . 1920 1933 + AjuI GAANNNNNNNTTGG 1912 1907 1944 1939 1920 1924 - Hin4II* CCTTC 1914 1913 . . 1944 1949 + DsaI* CCRYGG 1944 1948 . . 1944 1949 + SecI* CCNNGG 1944 1948 . . 1954 1958 + Hpy188I TCNGA 1956 1955 . . 1964 1967 + Csp6I GTAC 1964 1966 . . 1964 1967 + RsaI GTAC 1965 1965 . . 1973 1977 + PleI GAGTC 1981 1982 . . 1973 1977 + MlyI GAGTC 1982 1982 . . 1973 1977 + HinfI GANTC 1973 1976 . . 1975 1979 + Tsp45I GTSAC 1974 1979 . . 1975 1979 + MaeIII GTNAC 1974 1979 . . 1980 1985 + Hpy178III* TCNNGA 1981 1983 . . 1984 1988 + MboII GAAGA 1996 1995 . . 1979 1984 - Eco57MI CTGRAG 1964 1962 . . 1979 1984 - GsuI CTGGAG 1964 1962 . . 1992 1997 + XhoII RGATCY 1992 1996 . . 1992 1997 + BglII AGATCT 1992 1996 . . 1993 1996 + DpnI GATC 1994 1994 . . 1993 1996 + BstKTI GATC 1995 1993 . . 1993 1996 + MboI GATC 1992 1996 . . 1996 1999 + MaeI CTAG 1996 1998 . . 1998 2001 + SetI ASST 2001 1997 . . 2025 2028 + Csp6I GTAC 2025 2027 . . 2025 2028 + RsaI GTAC 2026 2026 . . 2029 2034 + ApoI RAATTY 2029 2033 . . 2030 2033 + TspEI AATT 2029 2033 . . 2035 2039 - BccI CCATC 2029 2030 . . 2042 2047 + BclI TGATCA 2042 2046 . . 2043 2046 + DpnI GATC 2044 2044 . . 2043 2046 + BstKTI GATC 2045 2043 . . 2043 2046 + MboI GATC 2042 2046 . . 2059 2064 + AsuII TTCGAA 2060 2062 . . 2060 2063 + TaqI TCGA 2060 2062 . . 2063 2068 + HindIII AAGCTT 2063 2067 . . 2064 2067 + SetI ASST 2067 2063 . . 2064 2067 + CviJI RGCY 2065 2065 . . 2064 2067 + AluI AGCT 2065 2065 . . 2071 2075 + TfiI GAWTC 2071 2074 . . 2071 2075 + HinfI GANTC 2071 2074 . . 2084 2087 + TspEI AATT 2083 2087 . . 2112 2115 + SetI ASST 2115 2111 . . 2112 2115 + CviJI RGCY 2113 2113 . . 2112 2115 + AluI AGCT 2113 2113 . . 2117 2121 + Hpy188I TCNGA 2119 2118 . . 2120 2124 + TfiI GAWTC 2120 2123 . . 2120 2124 + HinfI GANTC 2120 2123 . . 2132 2137 + Hpy178III* TCNNGA 2133 2135 . . 2142 2145 + SetI ASST 2145 2141 . . 2142 2145 + CviJI RGCY 2143 2143 . . 2142 2145 + AluI AGCT 2143 2143 . . 2164 2168 + BssKI CCNGG 2163 2168 . . 2164 2168 + BseBI CCWGG 2165 2166 . . 2164 2168 + ScrFI CCNGG 2165 2166 . . 2164 2168 + EcoRII CCWGG 2163 2168 . . 2165 2173 + AlwNI CAGNNNCTG 2170 2167 . . 2166 2169 + SetI ASST 2169 2165 . . 2168 2171 + Csp6I GTAC 2168 2170 . . 2168 2171 + RsaI GTAC 2169 2169 . . 2188 2192 + Hpy188I TCNGA 2190 2189 . . 2195 2198 + AciI CCGC 2195 2197 . . 2197 2207 + MwoI GCNNNNNNNGC 2203 2200 . . 2202 2207 + BsmI GAATGC 2208 2206 . . 2219 2223 + Tsp4CI* ACNGT 2221 2220 . . 2231 2234 + MseI TTAA 2231 2233 . . 2240 2243 + AciI CCGC 2240 2242 . . 2245 2249 - MboII GAAGA 2237 2236 . . 2256 2259 + SetI ASST 2259 2255 . . 2255 2258 - MnlI CCTC 2248 2247 . . 2262 2265 + CviJI RGCY 2263 2263 . . 2264 2268 + Hin4II* CCTTC 2274 2273 . . 2273 2276 + TaqI TCGA 2273 2275 . . 2283 2286 + SetI ASST 2286 2282 . . 2288 2292 + MaeIII GTNAC 2287 2292 . . 2292 2296 + DdeI CTNAG 2292 2295 . . 2292 2296 + BspCNI CTCAG 2305 2303 . . 2292 2296 + BseMII CTCAG 2306 2304 . . 2296 2299 + CviJI RGCY 2297 2297 . . 2303 2308 + BalI TGGCCA 2305 2305 . . 2303 2308 + CfrI YGGCCR 2303 2307 . . 2304 2307 + CviJI RGCY 2305 2305 . . 2304 2307 + HaeIII GGCC 2305 2305 . . 2305 2315 + MwoI GCNNNNNNNGC 2311 2308 . . 2314 2324 + MwoI GCNNNNNNNGC 2320 2317 . . 2322 2325 + CviJI RGCY 2323 2323 . . 2323 2333 + MwoI GCNNNNNNNGC 2329 2326 . . 2330 2335 + SduI GDGCHC 2334 2330 . . 2330 2335 + HgiAI* GWGCWC 2334 2330 . . 2333 2338 + SmlI CTYRAG 2333 2337 . . 2333 2338 - Bce83I* CTTGAG 2318 2316 . . 2338 2342 + BsmAI GTCTC 2343 2347 . . 2350 2360 + BsiYI* CCNNNNNNNGG 2356 2353 . . 2347 2351 - BslFI GGGAC 2332 2336 . . 2353 2357 + MboII GAAGA 2365 2364 . . 2358 2361 + SetI ASST 2361 2357 . . 2356 2360 - Hin4II* CCTTC 2350 2349 . . 2374 2379 + MfeI CAATTG 2374 2378 . . 2375 2378 + TspEI AATT 2374 2378 . . 2380 2384 + Hpy188I TCNGA 2382 2381 . . 2370 2380 - BsaXI ACNNNNNCTCC 2392 2389 2362 2359 2371 2381 - Hin4I GAYNNNNNVTC 2394 2389 2362 2357 2381 2386 + SecI* CCNNGG 2381 2385 . . 2383 2386 - MnlI CCTC 2376 2375 . . 2397 2401 + DdeI CTNAG 2397 2400 . . 2400 2403 + SetI ASST 2403 2399 . . 2400 2403 + CviJI RGCY 2401 2401 . . 2400 2403 + AluI AGCT 2401 2401 . . 2424 2427 + SetI ASST 2427 2423 . . 2422 2426 - Hin4II* CCTTC 2416 2415 . . 2430 2433 + SetI ASST 2433 2429 . . 2429 2432 - MnlI CCTC 2422 2421 . . 2444 2447 + TspEI AATT 2443 2447 . . 2454 2458 + MboII GAAGA 2466 2465 . . 2458 2463 + TatI WGTACW 2458 2462 . . 2459 2462 + Csp6I GTAC 2459 2461 . . 2454 2459 - Ksp632I* CTCTTC 2449 2452 . . 2459 2462 + RsaI GTAC 2460 2460 . . 2467 2477 + MwoI GCNNNNNNNGC 2473 2470 . . 2471 2475 + DdeI CTNAG 2471 2474 . . 2459 2471 - BarI GAAGNNNNNNTAC 2483 2478 2451 2446 2475 2478 + CviJI RGCY 2476 2476 . . 2476 2486 + MwoI GCNNNNNNNGC 2482 2479 . . 2484 2487 + CviJI RGCY 2485 2485 . . 2486 2497 + BstXI CCANNNNNNTGG 2493 2489 . . 2492 2495 + TspEI AATT 2491 2495 . . 2496 2499 + CviJI RGCY 2497 2497 . . 2498 2503 + Eco57MI CTGRAG 2519 2517 . . 2498 2503 + Eco57I CTGAAG 2519 2517 . . 2513 2518 + Eco57MI CTGRAG 2534 2532 . . 2513 2518 + Eco57I CTGAAG 2534 2532 . . 2510 2515 - EcoP15I CAGCAG 2482 2484 . . 2517 2520 + CviJI RGCY 2518 2518 . . 2521 2525 - BccI CCATC 2515 2516 . . 2533 2537 + HgaI GACGC 2542 2547 . . 2536 2546 + MwoI GCNNNNNNNGC 2542 2539 . . 2536 2542 + EspI* GCTNAGC 2537 2540 . . 2537 2541 + DdeI CTNAG 2537 2540 . . 2540 2543 + CviJI RGCY 2541 2541 . . 2541 2546 + Cac8I GCNNGC 2543 2543 . . 2544 2547 + SetI ASST 2547 2543 . . 2544 2547 + CviJI RGCY 2545 2545 . . 2544 2547 + AluI AGCT 2545 2545 . . 2551 2555 + BssKI CCNGG 2550 2555 . . 2551 2555 + BseBI CCWGG 2552 2553 . . 2551 2555 + ScrFI CCNGG 2552 2553 . . 2551 2555 + EcoRII CCWGG 2550 2555 . . 2553 2556 + SetI ASST 2556 2552 . . 2557 2560 + FatI CATG 2556 2560 . . 2557 2560 + NlaIII CATG 2560 2556 . . 2557 2560 + CviAII CATG 2557 2559 . . 2559 2564 + TatI WGTACW 2559 2563 . . 2559 2564 + Bsp1407I TGTACA 2559 2563 . . 2560 2563 + Csp6I GTAC 2560 2562 . . 2560 2563 + RsaI GTAC 2561 2561 . . 2568 2571 + SetI ASST 2571 2567 . . 2578 2582 + BccI CCATC 2586 2587 . . 2585 2590 + MfeI CAATTG 2585 2589 . . 2586 2589 + TspEI AATT 2585 2589 . . 2601 2604 + CviJI RGCY 2602 2602 . . 2603 2614 + BstXI CCANNNNNNTGG 2610 2606 . . 2604 2613 + MslI CAYNNNNRTG 2608 2608 . . 2606 2609 + MaeI CTAG 2606 2608 . . 2612 2617 + BalI TGGCCA 2614 2614 . . 2612 2617 + CfrI YGGCCR 2612 2616 . . 2613 2616 + CviJI RGCY 2614 2614 . . 2613 2616 + HaeIII GGCC 2614 2614 . . 2614 2624 + MwoI GCNNNNNNNGC 2620 2617 . . 2618 2621 + MseI TTAA 2618 2620 . . 2622 2625 + SetI ASST 2625 2621 . . 2622 2625 + CviJI RGCY 2623 2623 . . 2622 2625 + AluI AGCT 2623 2623 . . 2629 2632 + CviJI RGCY 2630 2630 . . 2632 2636 - TspDTI ATGAA 2622 2620 . . 2649 2652 + SetI ASST 2652 2648 . . 2649 2652 + CviJI RGCY 2650 2650 . . 2649 2652 + AluI AGCT 2650 2650 . . 2650 2660 + MwoI GCNNNNNNNGC 2656 2653 . . 2650 2654 + BlsI GCNGC 2652 2651 . . 2650 2654 + BisI GCNGC 2651 2652 . . 2650 2654 + TseI GCWGC 2650 2653 . . 2650 2654 - BbvI GCAGC 2637 2641 . . 2660 2665 + Eco57MI CTGRAG 2681 2679 . . 2655 2660 - BsrDI GCAATG 2654 2652 . . 2660 2665 + Eco57I CTGAAG 2681 2679 . . 2664 2667 + SetI ASST 2667 2663 . . 2664 2667 + CviJI RGCY 2665 2665 . . 2664 2667 + AluI AGCT 2665 2665 . . 2665 2675 + MwoI GCNNNNNNNGC 2671 2668 . . 2671 2675 + BlsI GCNGC 2673 2672 . . 2671 2675 + BisI GCNGC 2672 2673 . . 2666 2671 - BsiI* CACGAG 2666 2670 . . 2671 2675 + TseI GCWGC 2671 2674 . . 2671 2675 - BbvI GCAGC 2658 2662 . . 2677 2681 + MboII GAAGA 2689 2688 . . 2710 2714 + Hpy188I TCNGA 2712 2711 . . 2715 2721 + TspRI CASTGNN 2721 2712 . . 2719 2723 + BlsI GCNGC 2721 2720 . . 2719 2723 + BisI GCNGC 2720 2721 . . 2719 2723 + TseI GCWGC 2719 2722 . . 2715 2720 - BtsI GCAGTG 2714 2712 . . 2717 2722 - EcoP15I CAGCAG 2689 2691 . . 2719 2723 - BbvI GCAGC 2706 2710 . . 2720 2725 - EcoP15I CAGCAG 2692 2694 . . 2735 2738 + MseI TTAA 2735 2737 . . 2741 2746 + Hpy166II GTNNAC 2743 2743 . . 2745 2750 + BspLU11I* ACATGT 2745 2749 . . 2745 2750 + NspI RCATGY 2749 2745 . . 2745 2750 + AflIII ACRYGT 2745 2749 . . 2746 2749 + FatI CATG 2745 2749 . . 2746 2749 + NlaIII CATG 2749 2745 . . 2746 2749 + CviAII CATG 2746 2748 . . 2752 2757 + Hpy166II GTNNAC 2754 2754 . . 2770 2775 + MfeI CAATTG 2770 2774 . . 2771 2774 + TspEI AATT 2770 2774 . . 2783 2787 + TspDTI ATGAA 2798 2796 . . 2789 2794 + Eco57MI CTGRAG 2810 2808 . . 2789 2794 + Eco57I CTGAAG 2810 2808 . . 2799 2808 + MslI CAYNNNNRTG 2803 2803 . . 2799 2802 + FatI CATG 2798 2802 . . 2799 2802 + NlaIII CATG 2802 2798 . . 2799 2802 + CviAII CATG 2799 2801 . . 2804 2809 + BsmI GAATGC 2810 2808 . . 2813 2816 + SetI ASST 2816 2812 . . 2821 2827 + TspRI CASTGNN 2827 2818 . . 2822 2826 + BsrI ACTGG 2827 2825 . . 2826 2831 + Cac8I GCNNGC 2828 2828 . . 2829 2832 + SetI ASST 2832 2828 . . 2829 2832 + CviJI RGCY 2830 2830 . . 2829 2832 + AluI AGCT 2830 2830 . . 2837 2841 + HinfI GANTC 2837 2840 . . 2837 2841 - PleI GAGTC 2831 2832 . . 2837 2841 - MlyI GAGTC 2831 2831 . . 2841 2846 - BsrDI GCAATG 2840 2838 . . 2850 2861 + BstXI CCANNNNNNTGG 2857 2853 . . 2861 2865 + MboII GAAGA 2873 2872 . . 2857 2862 - MmeI TCCRAC 2838 2836 . . 2877 2880 + MseI TTAA 2877 2879 . . #--------------------------------------- # # # AatII: # [GACGTC] # 621 764 1495 # # Acc65I: # [GGTACC] # 787 2093 # # AccI: # [GTMKAC] # 1066 1814 # # AciI: # [CCGC] # 45 117 195 360 545 640 # 978 # # AclI: # [AACGTT] # 234 407 2239 # # AcyI: # [GRCGYC] # 621 761 1498 # # AflIII: # [ACRYGT] # 135 2745 # # AhaIII*: # [TTTAAA] # 1186 1694 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 713 936 1167 # # AluI: # [AGCT] # 9 15 18 27 30 42 # 48 50 52 76 78 83 # 144 165 258 273 399 438 # 675 # # AlwNI: # [CAGNNNCTG] # 710 1070 1100 # # ApoI: # [RAATTY] # 72 160 745 851 1052 # # AsuI*: # [GGNCC] # 334 393 2153 # # AsuII: # [TTCGAA] # 820 2060 # # AvaII: # [GGWCC] # 334 393 2153 # # BaeI: # [ACNNNNGTAYC] # 13 13 20 1386 1448 # # BalI: # [TGGCCA] # 266 309 2305 # # BarI: # [GAAGNNNNNNTAC] # 32 32 397 1197 1222 # # BbvI: # [GCAGC] # 21 48 63 174 1275 1299 # # BbvII*: # [GAAGAC] # 764 2116 # # BccI: # [CCATC] # 71 94 169 214 264 294 # 395 434 459 486 # # Bce83I*: # [CTTGAG] # 236 394 562 796 892 # # BceAI: # [ACGGC] # 9 294 583 909 1085 # # BciVI: # [GTATCC] # 1410 1470 # # BclI: # [TGATCA] # 838 2042 # # BetI*: # [WCCGGW] # 610 2270 # # BfiI: # [ACTGGG] # 762 2118 # # BglII: # [AGATCT] # 888 1992 # # BinI*: # [GGATC] # 168 2712 # # BisI: # [GCNGC] # 21 48 77 160 1275 1299 # # BlsI: # [GCNGC] # 21 48 78 159 1275 1299 # # BmgT120I: # [GGNCC] # 334 394 2152 # # Bpu10I: # [CCTNAGC] # 1240 1640 # # BsaXI: # [ACNNNNNCTCC] # 30 30 488 1059 1273 # # BseBI: # [CCWGG] # 328 387 626 1539 # # BseGI: # [GGATG] # 1068 1812 # # BseMII: # [CTCAG] # 473 574 639 1194 # # BseRI: # [GAGGAG] # 332 2548 # # BseSI: # [GKGCMC] # 852 2028 # # BsiI*: # [CACGAG] # 214 2666 # # BsiYI*: # [CCNNNNNNNGG] # 67 220 282 313 449 464 # 524 561 # # BslFI: # [GGGAC] # 508 548 1824 # # BsmAI: # [GTCTC] # 85 371 537 594 1293 # # BsmI: # [GAATGC] # 70 602 2208 # # Bsp1407I: # [TGTACA] # 321 383 2176 # # BspCNI: # [CTCAG] # 474 575 637 1194 # # BspHI: # [TCATGA] # 602 2278 # # BspLU11I*: # [ACATGT] # 135 2745 # # BsrBI: # [CCGCTC] # 119 2761 # # BsrDI: # [GCAATG] # 40 186 2654 # # BsrI: # [ACTGG] # 53 161 262 495 906 1003 # # BssKI: # [CCNGG] # 330 387 624 1539 # # BstAPI: # [GCANNNNNTGC] # 1342 1538 # # BstKTI: # [GATC] # 10 19 31 37 50 89 # 176 319 622 692 835 # # BstXI: # [CCANNNNNNTGG] # 23 117 247 2493 # # BtsI: # [GCAGTG] # 47 166 1080 1587 # # Cac8I: # [GCNNGC] # 52 285 1247 1296 # # Cfr10I: # [RCCGGY] # 868 2012 # # CfrI: # [YGGCCR] # 268 309 2303 # # ClaI: # [ATCGAT] # 1112 1768 # # Csp6I: # [GTAC] # 12 12 29 61 101 143 # 225 291 320 343 404 432 # 507 # # CspCI: # [CAANNNNNGTGG] # 35 1308 1537 # # CviAII: # [CATG] # 53 81 93 189 190 268 # 335 663 1008 # # CviJI: # [RGCY] # 4 4 7 8 9 9 # 9 9 12 12 12 15 # 18 18 18 20 21 21 # 23 24 25 30 32 33 # 34 42 44 44 48 50 # 51 51 52 57 67 74 # 75 76 78 97 120 123 # 136 141 165 166 219 237 # 240 # # CviRI*: # [TGCA] # 52 288 326 958 1256 # # DdeI: # [CTNAG] # 36 54 66 74 105 206 # 343 428 616 952 # # DpnI: # [GATC] # 10 19 31 37 50 89 # 175 319 622 692 836 # # DsaI*: # [CCRYGG] # 293 765 886 936 # # Eco57I: # [CTGAAG] # 15 70 129 147 739 800 # 980 # # Eco57MI: # [CTGRAG] # 8 15 70 110 129 147 # 169 184 192 255 329 555 # 717 # # EcoNI: # [CCTNNNNNAGG] # 347 2533 # # EcoP15I: # [CAGCAG] # 3 108 188 207 258 297 # 451 576 792 # # EcoRI: # [GAATTC] # 72 745 2063 # # EcoRII: # [CCWGG] # 330 387 624 1539 # # EspI*: # [GCTNAGC] # 343 2537 # # FatI: # [CATG] # 53 82 93 189 190 267 # 335 663 1008 # # FnuDII*: # [CGCG] # 830 2050 # # FokI: # [GGATG] # 1075 1805 # # GlaI: # [GCGC] # 829 2051 # # GsuI: # [CTGGAG] # 110 169 192 255 329 909 # 916 # # HaeIII: # [GGCC] # 74 166 266 286 309 399 # 582 798 # # HgaI: # [GACGC] # 338 2542 # # HgiAI*: # [GWGCWC] # 546 546 1788 # # HgiCI*: # [GGYRCC] # 60 787 2033 # # HhaI: # [GCGC] # 830 2050 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 5 27 # 27 32 32 32 32 67 # 121 223 486 802 994 # # Hin4II*: # [CCTTC] # 9 32 51 66 72 76 # 127 187 360 380 464 475 # 581 # # Hin6I: # [GCGC] # 828 2052 # # HindII: # [GTYRAC] # 47 162 1270 1401 # # HindIII: # [AAGCTT] # 473 817 1590 # # HinfI: # [GANTC] # 43 49 72 92 98 157 # 235 376 428 613 717 # # HpaI: # [GTTAAC] # 1317 1563 # # HpaII: # [CCGG] # 258 611 2011 # # HphI: # [GGTGA] # 282 628 628 1342 # # Hpy166II: # [GTNNAC] # 11 15 22 25 121 126 # 162 205 331 386 548 928 # # Hpy178III*: # [TCNNGA] # 33 60 66 92 92 116 # 127 127 152 264 323 331 # 350 747 # # Hpy188I: # [TCNGA] # 71 163 168 192 300 330 # 636 1020 # # Hpy99I: # [CGWCG] # 1384 1496 # # KpnI: # [GGTACC] # 791 2089 # # Ksp632I*: # [CTCTTC] # 126 224 431 2099 # # MaeI: # [CTAG] # 274 600 610 1396 # # MaeII: # [ACGT] # 60 120 183 224 234 264 # 357 1438 # # MaeIII: # [GTNAC] # 6 14 39 128 159 313 # 358 381 408 481 593 # # MboI: # [GATC] # 10 19 31 37 50 89 # 173 319 622 692 838 # # MboII: # [GAAGA] # 7 70 101 109 113 128 # 154 184 223 223 241 323 # 1004 # # McrI*: # [CGRYCG] # 1404 1476 # # MfeI: # [CAATTG] # 110 185 211 593 1781 # # MlyI: # [GAGTC] # 49 243 250 849 1489 # # MmeI: # [TCCRAC] # 42 323 684 1831 # # MnlI: # [CCTC] # 46 53 86 90 128 146 # 154 207 265 458 496 751 # # MseI: # [TTAA] # 3 27 29 51 75 82 # 117 142 290 387 456 1221 # # MslI: # [CAYNNNNRTG] # 77 195 710 1898 # # MwoI: # [GCNNNNNNNGC] # 9 9 9 9 9 9 # 15 36 60 78 108 114 # 123 126 135 144 162 209 # 337 567 612 # # NlaIII: # [CATG] # 53 78 93 189 190 271 # 335 663 1008 # # NlaIV: # [GGNNCC] # 60 394 395 2031 # # NspBII*: # [CMGCKG] # 573 1088 1219 # # NspI: # [RCATGY] # 131 271 2478 # # PflMI: # [CCANNNNNTGG] # 282 533 980 1085 # # PleI: # [GAGTC] # 49 243 249 850 1489 # # PsiI: # [TTATAA] # 71 2809 # # PsrI: # [GAACNNNNNNTAC] # 32 32 675 1017 1124 # # PvuII: # [CAGCTG] # 1088 1792 # # RsaI: # [GTAC] # 12 12 30 61 101 143 # 225 291 319 343 404 432 # 507 # # SapI: # [GCTCTTC] # 126 2754 # # SauI*: # [CCTNAGG] # 1434 1446 # # ScrFI: # [CCNGG] # 328 387 626 1539 # # SduI: # [GDGCHC] # 546 546 852 936 # # SecI*: # [CCNNGG] # 30 102 281 293 437 484 # 499 754 # # SetI: # [ASST] # 5 5 5 6 9 9 # 9 10 14 15 15 16 # 18 22 22 24 24 27 # 27 27 28 30 31 34 # 41 42 42 45 48 48 # 50 51 52 54 54 66 # 67 75 76 77 78 83 # 90 108 114 141 141 149 # 153 219 284 # # SfaNI: # [GCATC] # 186 258 609 1827 # # SmlI: # [CTYRAG] # 251 358 547 832 892 # # SspI: # [AATATT] # 1017 1863 # # StuI: # [AGGCCT] # 582 2298 # # StyI: # [CCWWGG] # 281 754 807 1038 # # TaiI: # [ACGT] # 60 120 186 224 234 264 # 357 1435 # # TaqI: # [TCGA] # 78 159 213 241 607 707 # 875 # # TaqII: # [CACCCA] # 289 546 2045 # # TatI: # [WGTACW] # 101 321 383 2075 # # TfiI: # [GAWTC] # 49 72 157 428 474 760 # 940 # # TseI: # [GCWGC] # 21 48 76 161 1275 1299 # # TsoI: # [TARCCA] # 117 222 298 1067 1176 # # Tsp45I: # [GTSAC] # 45 128 159 381 408 853 # 906 # # Tsp4CI*: # [ACNGT] # 6 39 125 151 193 294 # 297 357 372 387 659 # # TspDTI: # [ATGAA] # 21 60 76 82 96 143 # 146 176 201 218 296 653 # 712 # # TspEI: # [AATT] # 19 48 54 57 69 72 # 94 110 124 141 150 152 # 185 229 291 331 364 390 # # TspGWI: # [ACGGA] # 48 321 515 706 1290 # # TspRI: # [CASTGNN] # 53 54 106 235 377 468 # 546 1041 # # Tth111I: # [GACNNNGTC] # 1285 1595 # # VspI: # [ATTAAT] # 1289 1591 # # XcmI: # [CCANNNNNNNNNTGG] # 279 2601 # # XhoII: # [RGATCY] # 173 888 1819 # # XmnI: # [GAANNNNTTC] # 270 368 979 1263 # # ZraI: # [GACGTC] # 621 762 1497 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2880 # Reported_sequences: 1 # Reported_hitcount: 713 #---------------------------------------