Restriction Fragments for TOM40/YMR203W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

TOM40/YMR203W on chromosome XIII from coordinates 668492 to 669655.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26500.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 9 + CviRI* TGCA 7 7 . . 26 31 + StuI AGGCCT 28 28 . . 27 30 + CviJI RGCY 28 28 . . 27 30 + HaeIII GGCC 28 28 . . 25 28 - MnlI CCTC 18 17 . . 29 32 + MnlI CCTC 39 38 . . 35 40 + ApoI RAATTY 35 39 . . 36 39 + TspEI AATT 35 39 . . 48 52 + BssKI CCNGG 47 52 . . 48 52 + ScrFI CCNGG 49 50 . . 48 52 + CauII* CCSGG 49 50 . . 49 52 + HpaII CCGG 49 51 . . 51 54 + CviJI RGCY 52 52 . . 51 54 + HaeIII GGCC 52 52 . . 51 55 + BmgT120I GGNCC 52 53 . . 51 55 + AsuI* GGNCC 51 54 . . 80 85 + StyI CCWWGG 80 84 . . 80 85 + SecI* CCNNGG 80 84 . . 87 96 + XmnI GAANNNNTTC 91 91 . . 87 92 + ApoI RAATTY 87 91 . . 88 91 + TspEI AATT 87 91 . . 80 93 - AjuI GAANNNNNNNTTGG 105 100 73 68 92 96 - MboII GAAGA 84 83 . . 98 101 + MnlI CCTC 108 107 . . 96 101 - BseRI GAGGAG 87 85 . . 113 116 + MaeI CTAG 113 115 . . 115 118 + SetI ASST 118 114 . . 115 118 + CviJI RGCY 116 116 . . 115 118 + AluI AGCT 116 116 . . 129 134 + SnaBI TACGTA 131 131 . . 129 134 + BsaAI YACGTR 131 131 . . 130 133 + TaiI ACGT 133 129 . . 130 133 + SetI ASST 133 129 . . 130 133 + MaeII ACGT 130 132 . . 126 131 - BciVI GTATCC 120 119 . . 132 135 + Csp6I GTAC 132 134 . . 132 135 + RsaI GTAC 133 133 . . 139 144 + MfeI CAATTG 139 143 . . 140 143 + TspEI AATT 139 143 . . 143 146 + CviRI* TGCA 144 144 . . 144 149 - BsmI GAATGC 144 142 . . 167 170 + SetI ASST 170 166 . . 167 170 + CviJI RGCY 168 168 . . 167 170 + AluI AGCT 168 168 . . 178 182 + BssKI CCNGG 177 182 . . 178 182 + ScrFI CCNGG 179 180 . . 178 182 + CauII* CCSGG 179 180 . . 179 182 + HpaII CCGG 179 181 . . 181 186 + KpnI GGTACC 185 181 . . 181 186 + NlaIV GGNNCC 183 183 . . 181 186 + HgiCI* GGYRCC 181 185 . . 181 186 + Acc65I GGTACC 181 185 . . 182 185 + Csp6I GTAC 182 184 . . 182 185 + RsaI GTAC 183 183 . . 184 188 + Tsp4CI* ACNGT 186 185 . . 185 190 + DsaI* CCRYGG 185 189 . . 185 190 + SecI* CCNNGG 185 189 . . 194 197 + SetI ASST 197 193 . . 195 205 + EcoNI CCTNNNNNAGG 199 200 . . 195 205 + BsiYI* CCNNNNNNNGG 201 198 . . 208 212 + BsmAI GTCTC 213 217 . . 215 219 + Tsp45I GTSAC 214 219 . . 215 219 + MaeIII GTNAC 214 219 . . 218 221 + TaiI ACGT 221 217 . . 218 221 + SetI ASST 221 217 . . 218 221 + MaeII ACGT 218 220 . . 218 223 + AflIII ACRYGT 218 222 . . 217 222 - BtrI CACGTC 219 219 . . 228 232 + BsmAI GTCTC 233 237 . . 230 234 + DdeI CTNAG 230 233 . . 230 234 + BspCNI CTCAG 243 241 . . 230 234 + BseMII CTCAG 244 242 . . 245 249 + BssKI CCNGG 244 249 . . 245 248 + HpaII CCGG 245 247 . . 245 249 + ScrFI CCNGG 246 247 . . 245 249 + CauII* CCSGG 246 247 . . 242 246 - HphI GGTGA 234 233 . . 248 251 + CviJI RGCY 249 249 . . 250 254 + DdeI CTNAG 250 253 . . 255 258 + SetI ASST 258 254 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + AluI AGCT 256 256 . . 279 282 + FatI CATG 278 282 . . 279 282 + NlaIII CATG 282 278 . . 279 282 + CviAII CATG 279 281 . . 280 284 + TspDTI ATGAA 295 293 . . 282 286 + TfiI GAWTC 282 285 . . 282 286 + HinfI GANTC 282 285 . . 298 301 + SetI ASST 301 297 . . 299 302 + MnlI CCTC 309 308 . . 314 319 + SfeI* CTRYAG 314 318 . . 318 321 + SetI ASST 321 317 . . 319 324 + NlaIV GGNNCC 321 321 . . 329 334 - BsrDI GCAATG 328 326 . . 335 339 + DdeI CTNAG 335 338 . . 339 342 + Csp6I GTAC 339 341 . . 339 342 + RsaI GTAC 340 340 . . 341 351 + BsaXI ACNNNNNCTCC 331 328 361 358 343 353 + MwoI GCNNNNNNNGC 349 346 . . 343 348 - BsmI GAATGC 343 341 . . 350 353 + AciI CCGC 350 352 . . 352 362 + MwoI GCNNNNNNNGC 358 355 . . 352 362 + BstAPI GCANNNNNTGC 358 355 . . 371 374 + SetI ASST 374 370 . . 380 385 + SmlI CTYRAG 380 384 . . 384 387 + SetI ASST 387 383 . . 380 385 - Bce83I* CTTGAG 365 363 . . 392 395 + TaqI TCGA 392 394 . . 412 417 + BetI* WCCGGW 412 416 . . 413 416 + HpaII CCGG 413 415 . . 421 424 + MseI TTAA 421 423 . . 452 456 + DdeI CTNAG 452 455 . . 455 458 + SetI ASST 458 454 . . 457 462 + Hpy166II GTNNAC 459 459 . . 457 462 + HindII GTYRAC 459 459 . . 461 464 + SetI ASST 464 460 . . 463 468 + SfeI* CTRYAG 463 467 . . 468 473 + EcoRV GATATC 470 470 . . 472 476 + Hpy188I TCNGA 474 473 . . 477 482 + BalI TGGCCA 479 479 . . 477 482 + CfrI YGGCCR 477 481 . . 478 481 + CviJI RGCY 479 479 . . 478 481 + HaeIII GGCC 479 479 . . 475 479 - BccI CCATC 469 470 . . 516 519 + CviJI RGCY 517 517 . . 520 524 + Hpy188I TCNGA 522 521 . . 532 537 + Hpy166II GTNNAC 534 534 . . 536 539 + TaiI ACGT 539 535 . . 536 539 + SetI ASST 539 535 . . 536 539 + MaeII ACGT 536 538 . . 553 557 + Hin4II* CCTTC 563 562 . . 562 566 + Hpy188I TCNGA 564 563 . . 563 567 + DdeI CTNAG 563 566 . . 563 567 - BspCNI CTCAG 555 553 . . 563 567 - BseMII CTCAG 554 552 . . 567 571 - Hin4II* CCTTC 561 560 . . 574 579 + EcoRI GAATTC 574 578 . . 574 579 + ApoI RAATTY 574 578 . . 575 578 + TspEI AATT 574 578 . . 582 585 + SetI ASST 585 581 . . 594 597 + CviRI* TGCA 595 595 . . 595 599 + SfaNI GCATC 604 608 . . 613 617 + MaeIII GTNAC 612 617 . . 619 622 + MnlI CCTC 629 628 . . 617 622 - BseRI GAGGAG 608 606 . . 623 626 + TspEI AATT 622 626 . . 627 630 + SetI ASST 630 626 . . 627 630 + CviJI RGCY 628 628 . . 627 630 + AluI AGCT 628 628 . . 633 636 + SetI ASST 636 632 . . 652 657 + Hpy178III* TCNNGA 653 655 . . 651 656 - Eco57MI CTGRAG 636 634 . . 651 656 - GsuI CTGGAG 636 634 . . 662 666 + Tsp4CI* ACNGT 664 663 . . 667 672 + HaeII RGCGCY 671 667 . . 667 672 + Eco47III AGCGCT 669 669 . . 668 671 + Hin6I GCGC 668 670 . . 668 671 + GlaI GCGC 669 669 . . 668 671 + HhaI GCGC 670 668 . . 673 677 + BssKI CCNGG 672 677 . . 673 677 + BseBI CCWGG 674 675 . . 673 677 + ScrFI CCNGG 674 675 . . 673 677 + EcoRII CCWGG 672 677 . . 675 678 + SetI ASST 678 674 . . 671 676 - Eco57MI CTGRAG 656 654 . . 671 676 - GsuI CTGGAG 656 654 . . 676 680 + HphI GGTGA 688 687 . . 679 683 - SfaNI GCATC 669 673 . . 690 694 - TspDTI ATGAA 680 678 . . 699 703 + HinfI GANTC 699 702 . . 699 703 - PleI GAGTC 693 694 . . 699 703 - MlyI GAGTC 693 693 . . 709 713 + BsmAI GTCTC 714 718 . . 738 742 + BmgT120I GGNCC 739 740 . . 738 742 + AsuI* GGNCC 738 741 . . 739 742 + CviJI RGCY 740 740 . . 739 742 + HaeIII GGCC 740 740 . . 742 747 + MfeI CAATTG 742 746 . . 743 746 + TspEI AATT 742 746 . . 746 749 + CviRI* TGCA 747 747 . . 747 752 + Cac8I GCNNGC 749 749 . . 750 753 + CviJI RGCY 751 751 . . 751 761 + MwoI GCNNNNNNNGC 757 754 . . 755 759 + Tsp4CI* ACNGT 757 756 . . 760 770 + MwoI GCNNNNNNNGC 766 763 . . 763 766 + MseI TTAA 763 765 . . 765 768 + TspEI AATT 764 768 . . 768 771 + CviRI* TGCA 769 769 . . 769 773 + SfaNI GCATC 778 782 . . 770 779 + MslI CAYNNNNRTG 774 774 . . 770 775 - BtgZI GCGATG 755 759 . . 800 803 + TaqI TCGA 800 802 . . 802 805 - MnlI CCTC 795 794 . . 807 810 + SetI ASST 810 806 . . 805 810 - BspMI ACCTGC 796 800 . . 812 815 + TaqI TCGA 812 814 . . 828 831 + SetI ASST 831 827 . . 828 831 + CviJI RGCY 829 829 . . 828 831 + AluI AGCT 829 829 . . 837 842 + NlaIV GGNNCC 839 839 . . 850 853 + DpnI GATC 851 851 . . 850 853 + BstKTI GATC 852 850 . . 850 853 + MboI GATC 849 853 . . 853 863 + BsiYI* CCNNNNNNNGG 859 856 . . 850 854 - BinI* GGATC 844 845 . . 858 868 + Hin4I GAYNNNNNVTC 849 844 881 876 861 866 + SduI GDGCHC 865 861 . . 861 866 + BseSI GKGCMC 865 861 . . 858 862 - BccI CCATC 852 853 . . 869 874 + MfeI CAATTG 869 873 . . 870 873 + TspEI AATT 869 873 . . 882 885 + SetI ASST 885 881 . . 886 890 + Tsp4CI* ACNGT 888 887 . . 890 893 + TaqI TCGA 890 892 . . 892 895 - MnlI CCTC 885 884 . . 914 918 + DdeI CTNAG 914 917 . . 920 924 + TspDTI ATGAA 935 933 . . 947 950 + Csp6I GTAC 947 949 . . 947 950 + RsaI GTAC 948 948 . . 955 959 + TfiI GAWTC 955 958 . . 955 959 + HinfI GANTC 955 958 . . 968 971 + SetI ASST 971 967 . . 972 975 + CviRI* TGCA 973 973 . . 973 978 + NspI RCATGY 977 973 . . 974 977 + FatI CATG 973 977 . . 974 977 + NlaIII CATG 977 973 . . 974 977 + CviAII CATG 974 976 . . 981 986 + XbaI TCTAGA 981 985 . . 981 986 + Hpy178III* TCNNGA 982 984 . . 982 985 + MaeI CTAG 982 984 . . 1009 1013 - TspGWI ACGGA 999 997 . . 1024 1028 + HphI GGTGA 1036 1035 . . 1022 1025 - AciI CCGC 1022 1024 . . 1026 1037 + BdaI TGANNNNNNTCA 1015 1013 1049 1047 1030 1035 + ClaI ATCGAT 1031 1033 . . 1031 1034 + TaqI TCGA 1031 1033 . . 1033 1036 + DpnI GATC 1034 1034 . . 1033 1036 + BstKTI GATC 1035 1033 . . 1033 1036 + MboI GATC 1032 1036 . . 1026 1037 - BdaI TGANNNNNNTCA 1049 1047 1015 1013 1040 1045 + Hpy178III* TCNNGA 1041 1043 . . 1067 1072 + Hpy166II GTNNAC 1069 1069 . . 1064 1067 - AciI CCGC 1064 1066 . . 1067 1072 + AccI GTMKAC 1068 1070 . . 1073 1076 + TspEI AATT 1072 1076 . . 1082 1087 - EcoP15I CAGCAG 1054 1056 . . 1092 1097 + Hpy178III* TCNNGA 1093 1095 . . 1097 1100 + TspEI AATT 1096 1100 . . 1107 1111 + MaeIII GTNAC 1106 1111 . . 1116 1119 + SetI ASST 1119 1115 . . 1123 1127 + HgaI GACGC 1132 1137 . . 1132 1138 + BstEII GGTNACC 1132 1137 . . 1133 1137 + MaeIII GTNAC 1132 1137 . . 1129 1133 - BccI CCATC 1123 1124 . . 1142 1145 + CviRI* TGCA 1143 1143 . . 1143 1148 + Cac8I GCNNGC 1145 1145 . . 1139 1144 - BsrDI GCAATG 1138 1136 . . 1146 1149 + SetI ASST 1149 1145 . . 1146 1149 + CviJI RGCY 1147 1147 . . 1146 1149 + AluI AGCT 1147 1147 . . 1147 1153 + SapI GCTCTTC 1154 1157 . . 1148 1153 + Ksp632I* CTCTTC 1154 1157 . . 1153 1156 + MnlI CCTC 1163 1162 . . 1149 1153 - MboII GAAGA 1141 1140 . . 1156 1161 + MfeI CAATTG 1156 1160 . . 1157 1160 + TspEI AATT 1156 1160 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 181 983 # # AccI: # [GTMKAC] # 96 1068 # # AciI: # [CCGC] # 42 100 350 672 # # AflIII: # [ACRYGT] # 218 946 # # AjuI: # [GAANNNNNNNTTGG] # 32 73 1059 # # AluI: # [AGCT] # 17 52 88 116 201 318 # 372 # # ApoI: # [RAATTY] # 35 52 487 590 # # AsuI*: # [GGNCC] # 51 426 687 # # BalI: # [TGGCCA] # 479 685 # # BccI: # [CCATC] # 41 271 383 469 # # Bce83I*: # [CTTGAG] # 365 799 # # BciVI: # [GTATCC] # 120 1044 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 115 1015 # # BetI*: # [WCCGGW] # 412 752 # # BinI*: # [GGATC] # 320 844 # # BmgT120I: # [GGNCC] # 52 425 687 # # BsaAI: # [YACGTR] # 131 1033 # # BsaXI: # [ACNNNNNCTCC] # 30 331 803 # # BseBI: # [CCWGG] # 490 674 # # BseMII: # [CTCAG] # 244 310 610 # # BseRI: # [GAGGAG] # 87 521 556 # # BseSI: # [GKGCMC] # 299 865 # # BsiYI*: # [CCNNNNNNNGG] # 201 305 658 # # BsmAI: # [GTCTC] # 20 213 450 481 # # BsmI: # [GAATGC] # 144 199 821 # # BspCNI: # [CTCAG] # 243 312 609 # # BspMI: # [ACCTGC] # 368 796 # # BsrDI: # [GCAATG] # 26 328 810 # # BssKI: # [CCNGG] # 47 67 130 428 492 # # BstAPI: # [GCANNNNNTGC] # 358 806 # # BstEII: # [GGTNACC] # 32 1132 # # BstKTI: # [GATC] # 129 183 852 # # BtgZI: # [GCGATG] # 409 755 # # BtrI: # [CACGTC] # 219 945 # # Cac8I: # [GCNNGC] # 19 396 749 # # CauII*: # [CCSGG] # 49 67 130 918 # # CfrI: # [YGGCCR] # 477 687 # # ClaI: # [ATCGAT] # 133 1031 # # Csp6I: # [GTAC] # 50 132 157 217 608 # # CviAII: # [CATG] # 190 279 695 # # CviJI: # [RGCY] # 7 11 17 24 28 38 # 52 64 78 81 111 112 # 223 318 # # CviRI*: # [TGCA] # 7 21 22 137 152 170 # 204 451 # # DdeI: # [CTNAG] # 20 85 111 117 230 250 # 351 # # DpnI: # [GATC] # 130 183 851 # # DsaI*: # [CCRYGG] # 185 979 # # Eco47III: # [AGCGCT] # 495 669 # # Eco57MI: # [CTGRAG] # 20 508 636 # # EcoNI: # [CCTNNNNNAGG] # 199 965 # # EcoP15I: # [CAGCAG] # 110 1054 # # EcoRI: # [GAATTC] # 574 590 # # EcoRII: # [CCWGG] # 492 672 # # EcoRV: # [GATATC] # 470 694 # # FatI: # [CATG] # 191 278 695 # # GlaI: # [GCGC] # 495 669 # # GsuI: # [CTGGAG] # 20 508 636 # # HaeII: # [RGCGCY] # 493 671 # # HaeIII: # [GGCC] # 24 28 261 424 427 # # HgaI: # [GACGC] # 32 1132 # # HgiCI*: # [GGYRCC] # 181 983 # # HhaI: # [GCGC] # 494 670 # # Hin4I: # [GAYNNNNNVTC] # 32 283 849 # # Hin4II*: # [CCTTC] # 2 561 601 # # Hin6I: # [GCGC] # 496 668 # # HindII: # [GTYRAC] # 459 705 # # HinfI: # [GANTC] # 209 256 282 417 # # HpaII: # [CCGG] # 49 66 130 168 751 # # HphI: # [GGTGA] # 128 234 348 454 # # Hpy166II: # [GTNNAC] # 75 95 459 535 # # Hpy178III*: # [TCNNGA] # 52 59 71 329 653 # # Hpy188I: # [TCNGA] # 42 48 474 600 # # KpnI: # [GGTACC] # 185 979 # # Ksp632I*: # [CTCTTC] # 10 1154 # # MaeI: # [CTAG] # 113 182 869 # # MaeII: # [ACGT] # 88 130 318 628 # # MaeIII: # [GTNAC] # 26 32 214 398 494 # # MboI: # [GATC] # 132 183 849 # # MboII: # [GAAGA] # 23 84 1057 # # MfeI: # [CAATTG] # 8 127 139 287 603 # # MlyI: # [GAGTC] # 471 693 # # MnlI: # [CCTC] # 1 18 21 69 90 166 # 201 278 320 # # MseI: # [TTAA] # 342 401 421 # # MslI: # [CAYNNNNRTG] # 390 774 # # MwoI: # [GCNNNNNNNGC] # 9 9 349 398 399 # # NlaIII: # [CATG] # 187 282 695 # # NlaIV: # [GGNNCC] # 138 183 325 518 # # NspI: # [RCATGY] # 187 977 # # PleI: # [GAGTC] # 471 693 # # RsaI: # [GTAC] # 50 133 157 216 608 # # SapI: # [GCTCTTC] # 10 1154 # # ScrFI: # [CCNGG] # 49 67 130 428 490 # # SduI: # [GDGCHC] # 299 865 # # SecI*: # [CCNNGG] # 80 105 979 # # SetI: # [ASST] # 6 6 13 15 15 20 # 21 24 27 30 37 37 # 42 43 45 46 53 54 # 71 75 86 118 132 148 # # SfaNI: # [GCATC] # 65 109 386 604 # # SfeI*: # [CTRYAG] # 149 314 701 # # SmlI: # [CTYRAG] # 380 784 # # SnaBI: # [TACGTA] # 131 1033 # # StuI: # [AGGCCT] # 28 1136 # # StyI: # [CCWWGG] # 80 1084 # # TaiI: # [ACGT] # 88 133 318 625 # # TaqI: # [TCGA] # 12 78 133 141 392 408 # # TfiI: # [GAWTC] # 209 282 673 # # Tsp45I: # [GTSAC] # 214 950 # # Tsp4CI*: # [ACNGT] # 93 131 186 276 478 # # TspDTI: # [ATGAA] # 229 255 295 385 # # TspEI: # [AATT] # 8 22 24 35 48 52 # 52 60 105 120 203 435 # # TspGWI: # [ACGGA] # 165 999 # # XbaI: # [TCTAGA] # 183 981 # # XmnI: # [GAANNNNTTC] # 91 1073 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1164 # Reported_sequences: 1 # Reported_hitcount: 266 #---------------------------------------