Restriction Fragments for YMR194C-A



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The currently selected gene/sequence is :

YMR194C-A on chromosome XIII from coordinates 653136 to 652912.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28743.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 13 + Hpy99I CGWCG 13 8 . . 16 21 + SspI AATATT 18 18 . . 24 34 + MwoI GCNNNNNNNGC 30 27 . . 32 35 + CviRI* TGCA 33 33 . . 34 39 + BsaAI YACGTR 36 36 . . 34 39 + PmaCI CACGTG 36 36 . . 35 38 + TaiI ACGT 38 34 . . 35 38 + SetI ASST 38 34 . . 35 38 + MaeII ACGT 35 37 . . 37 42 + Hpy166II GTNNAC 39 39 . . 42 46 + Hpy99I CGWCG 46 41 . . 43 48 + ZraI GACGTC 45 45 . . 43 48 + AcyI GRCGYC 44 46 . . 43 48 + AatII GACGTC 47 43 . . 44 47 + TaiI ACGT 47 43 . . 44 47 + SetI ASST 47 43 . . 44 47 + MaeII ACGT 44 46 . . 46 50 - BslFI GGGAC 31 35 . . 57 62 + ApoI RAATTY 57 61 . . 58 61 + TspEI AATT 57 61 . . 71 76 + Cac8I GCNNGC 73 73 . . 71 74 - AciI CCGC 71 73 . . 69 74 - BsrBI CCGCTC 71 71 . . 71 75 - FauI CCCGC 64 66 . . 75 80 + Cac8I GCNNGC 77 77 . . 68 78 - BsaXI ACNNNNNCTCC 90 87 60 57 68 79 - TstI CACNNNNNNTCC 92 87 60 55 84 89 + Hpy166II GTNNAC 86 86 . . 84 89 + HpaI GTTAAC 86 86 . . 84 89 + HindII GTYRAC 86 86 . . 85 88 + MseI TTAA 85 87 . . 88 91 + TaiI ACGT 91 87 . . 88 91 + SetI ASST 91 87 . . 88 91 + MaeII ACGT 88 90 . . 92 96 - FokI GGATG 78 82 . . 92 96 - BseGI GGATG 91 89 . . 94 98 - TspGWI ACGGA 84 82 . . 103 107 - BsmAI GTCTC 97 101 . . 112 116 + TspGWI ACGGA 127 125 . . 127 132 + TaqII CACCCA 143 141 . . 130 135 + DsaI* CCRYGG 130 134 . . 130 135 + StyI CCWWGG 130 134 . . 130 135 + NcoI CCATGG 130 134 . . 130 135 + SecI* CCNNGG 130 134 . . 126 130 - HphI GGTGA 118 117 . . 131 134 + FatI CATG 130 134 . . 131 134 + NlaIII CATG 134 130 . . 131 134 + CviAII CATG 131 133 . . 151 155 + Hpy188I TCNGA 153 152 . . 154 158 + MboII GAAGA 166 165 . . 154 159 - Ksp632I* CTCTTC 149 152 . . 157 160 - MnlI CCTC 150 149 . . 168 172 - BsmAI GTCTC 162 166 . . 175 180 + SspI AATATT 177 177 . . 181 185 + Tsp4CI* ACNGT 183 182 . . #--------------------------------------- # # # AatII: # [GACGTC] # 47 178 # # AciI: # [CCGC] # 71 154 # # AcyI: # [GRCGYC] # 44 181 # # ApoI: # [RAATTY] # 57 168 # # BsaAI: # [YACGTR] # 36 189 # # BsaXI: # [ACNNNNNCTCC] # 30 60 135 # # BseGI: # [GGATG] # 91 134 # # BslFI: # [GGGAC] # 31 194 # # BsmAI: # [GTCTC] # 63 65 97 # # BsrBI: # [CCGCTC] # 71 154 # # Cac8I: # [GCNNGC] # 4 73 148 # # CviAII: # [CATG] # 94 131 # # CviRI*: # [TGCA] # 33 192 # # DsaI*: # [CCRYGG] # 95 130 # # FatI: # [CATG] # 95 130 # # FauI: # [CCCGC] # 64 161 # # FokI: # [GGATG] # 78 147 # # HindII: # [GTYRAC] # 86 139 # # HpaI: # [GTTAAC] # 86 139 # # HphI: # [GGTGA] # 107 118 # # Hpy166II: # [GTNNAC] # 39 47 139 # # Hpy188I: # [TCNGA] # 72 153 # # Hpy99I: # [CGWCG] # 13 33 179 # # Ksp632I*: # [CTCTTC] # 76 149 # # MaeII: # [ACGT] # 9 35 44 137 # # MboII: # [GAAGA] # 59 166 # # MnlI: # [CCTC] # 75 150 # # MseI: # [TTAA] # 85 140 # # MwoI: # [GCNNNNNNNGC] # 30 195 # # NcoI: # [CCATGG] # 95 130 # # NlaIII: # [CATG] # 91 134 # # PmaCI: # [CACGTG] # 36 189 # # SecI*: # [CCNNGG] # 95 130 # # SetI: # [ASST] # 9 38 44 134 # # SspI: # [AATATT] # 18 48 159 # # StyI: # [CCWWGG] # 95 130 # # TaiI: # [ACGT] # 9 38 44 134 # # TaqII: # [CACCCA] # 82 143 # # Tsp4CI*: # [ACNGT] # 42 183 # # TspEI: # [AATT] # 57 168 # # TspGWI: # [ACGGA] # 43 84 98 # # TstI: # [CACNNNNNNTCC] # 32 60 133 # # ZraI: # [GACGTC] # 45 180 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 225 # Reported_sequences: 1 # Reported_hitcount: 55 #---------------------------------------