Restriction Fragments for SWP1/YMR149W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SWP1/YMR149W on chromosome XIII from coordinates 560996 to 561856.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29975.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 5 8 + TspEI AATT 4 8 . . 22 26 + TauI GCSGC 25 22 . . 22 26 + BlsI GCNGC 24 23 . . 22 26 + BisI GCNGC 23 24 . . 24 27 + CviJI RGCY 25 25 . . 24 27 + HaeIII GGCC 25 25 . . 22 25 - AciI CCGC 22 24 . . 26 31 + StyI CCWWGG 26 30 . . 26 31 + SecI* CCNNGG 26 30 . . 37 40 + CviRI* TGCA 38 38 . . 38 49 - BcgI CGANNNNNNTGC 61 59 27 25 51 54 + MnlI CCTC 61 60 . . 55 65 + MwoI GCNNNNNNNGC 61 58 . . 58 63 + BsaAI YACGTR 60 60 . . 59 62 + TaiI ACGT 62 58 . . 59 62 + SetI ASST 62 58 . . 59 62 + MaeII ACGT 59 61 . . 76 79 + FatI CATG 75 79 . . 76 79 + NlaIII CATG 79 75 . . 76 79 + CviAII CATG 76 78 . . 74 78 - TspDTI ATGAA 64 62 . . 89 92 + MnlI CCTC 99 98 . . 95 98 + AciI CCGC 95 97 . . 107 110 + MaeI CTAG 107 109 . . 117 120 + DpnI GATC 118 118 . . 117 120 + BstKTI GATC 119 117 . . 117 120 + MboI GATC 116 120 . . 133 143 + BsiYI* CCNNNNNNNGG 139 136 . . 143 147 + TfiI GAWTC 143 146 . . 143 147 + HinfI GANTC 143 146 . . 145 150 + ClaI ATCGAT 146 148 . . 146 149 + TaqI TCGA 146 148 . . 149 153 + TspDTI ATGAA 164 162 . . 151 160 + XmnI GAANNNNTTC 155 155 . . 154 158 + Tsp4CI* ACNGT 156 155 . . 159 164 + MmeI TCCRAC 184 182 . . 159 163 + Hpy188I TCNGA 161 160 . . 172 176 + Tsp4CI* ACNGT 174 173 . . 178 182 + MboII GAAGA 190 189 . . 178 183 + BbvII* GAAGAC 185 189 . . 183 193 + BsiYI* CCNNNNNNNGG 189 186 . . 191 194 + SetI ASST 194 190 . . 192 196 + HphI GGTGA 204 203 . . 190 193 - MnlI CCTC 183 182 . . 195 199 + TfiI GAWTC 195 198 . . 195 199 + HinfI GANTC 195 198 . . 204 209 + ApoI RAATTY 204 208 . . 205 208 + TspEI AATT 204 208 . . 217 221 + PleI GAGTC 225 226 . . 217 221 + MlyI GAGTC 226 226 . . 217 221 + HinfI GANTC 217 220 . . 219 224 + Hpy166II GTNNAC 221 221 . . 219 224 + AccI GTMKAC 220 222 . . 233 237 + Hin4II* CCTTC 243 242 . . 236 241 + Hpy178III* TCNNGA 237 239 . . 224 237 - AjuI GAANNNNNNNTTGG 249 244 217 212 244 247 + SetI ASST 247 243 . . 256 259 + CviJI RGCY 257 257 . . 261 264 + SetI ASST 264 260 . . 272 275 + SetI ASST 275 271 . . 273 283 + BsiYI* CCNNNNNNNGG 279 276 . . 274 277 + MaeI CTAG 274 276 . . 282 285 + CviJI RGCY 283 283 . . 297 300 + TspEI AATT 296 300 . . 299 302 + MseI TTAA 299 301 . . 321 324 + FatI CATG 320 324 . . 321 324 + NlaIII CATG 324 320 . . 321 324 + CviAII CATG 321 323 . . 344 349 + TsoI TARCCA 360 358 . . 345 348 + CviJI RGCY 346 346 . . 350 353 + SetI ASST 353 349 . . 350 353 + CviJI RGCY 351 351 . . 350 353 + AluI AGCT 351 351 . . 354 358 + FokI GGATG 367 371 . . 354 358 + BseGI GGATG 360 358 . . 358 362 + BlsI GCNGC 360 359 . . 358 362 + BisI GCNGC 359 360 . . 358 362 + TseI GCWGC 358 361 . . 355 359 - SfaNI GCATC 345 349 . . 358 362 - BbvI GCAGC 345 349 . . 368 371 + CviRI* TGCA 369 369 . . 375 378 + CviJI RGCY 376 376 . . 373 376 - MnlI CCTC 366 365 . . 379 384 + Hpy178III* TCNNGA 380 382 . . 383 386 + DpnI GATC 384 384 . . 383 386 + BstKTI GATC 385 383 . . 383 386 + MboI GATC 382 386 . . 387 392 + BetI* WCCGGW 387 391 . . 388 391 + HpaII CCGG 388 390 . . 385 389 - HphI GGTGA 377 376 . . 390 395 + NlaIV GGNNCC 392 392 . . 402 405 + CviJI RGCY 403 403 . . 402 405 + HaeIII GGCC 403 403 . . 411 415 + TfiI GAWTC 411 414 . . 411 415 + HinfI GANTC 411 414 . . 415 418 + MaeI CTAG 415 417 . . 418 422 - HgaI GACGC 407 412 . . 428 431 + AciI CCGC 428 430 . . 439 449 + FalI AAGNNNNNCTT 430 425 462 457 439 449 - FalI AAGNNNNNCTT 462 457 430 425 441 453 - AloI GAACNNNNNNTCC 465 460 433 428 458 463 + ApoI RAATTY 458 462 . . 459 462 + TspEI AATT 458 462 . . 464 467 + CviRI* TGCA 465 465 . . 473 476 + SetI ASST 476 472 . . 482 485 + TaqI TCGA 482 484 . . 484 493 + BsaBI GATNNNNATC 488 488 . . 490 493 + DpnI GATC 491 491 . . 490 493 + BstKTI GATC 492 490 . . 490 493 + MboI GATC 489 493 . . 495 499 + DdeI CTNAG 495 498 . . 495 499 + BspCNI CTCAG 508 506 . . 495 499 + BseMII CTCAG 509 507 . . 496 500 + Hpy188I TCNGA 498 497 . . 499 503 + TfiI GAWTC 499 502 . . 499 503 + HinfI GANTC 499 502 . . 501 505 - TspDTI ATGAA 491 489 . . 511 516 + Hpy166II GTNNAC 513 513 . . 511 516 + HindII GTYRAC 513 513 . . 517 522 + ApoI RAATTY 517 521 . . 518 521 + TspEI AATT 517 521 . . 527 530 + MseI TTAA 527 529 . . 530 533 + CviJI RGCY 531 531 . . 540 544 + BccI CCATC 548 549 . . 553 556 + FatI CATG 552 556 . . 553 556 + NlaIII CATG 556 552 . . 553 556 + CviAII CATG 553 555 . . 551 555 - TspDTI ATGAA 541 539 . . 556 561 + NmeAIII GCCGAG 582 580 . . 560 563 + CviJI RGCY 561 561 . . 581 586 + MfeI CAATTG 581 585 . . 582 585 + TspEI AATT 581 585 . . 601 604 + MseI TTAA 601 603 . . 603 606 + TspEI AATT 602 606 . . 608 612 - MboII GAAGA 600 599 . . 610 614 - TspDTI ATGAA 600 598 . . 628 631 + MseI TTAA 628 630 . . 634 638 + Tsp45I GTSAC 633 638 . . 634 638 + MaeIII GTNAC 633 638 . . 637 640 + SetI ASST 640 636 . . 637 643 + SexAI ACCWGGT 637 642 . . 638 642 + BssKI CCNGG 637 642 . . 638 642 + BseBI CCWGG 639 640 . . 638 642 + ScrFI CCNGG 639 640 . . 638 642 + EcoRII CCWGG 637 642 . . 635 639 - HphI GGTGA 627 626 . . 645 650 + EcoRI GAATTC 645 649 . . 645 650 + ApoI RAATTY 645 649 . . 646 649 + TspEI AATT 645 649 . . 655 659 + TauI GCSGC 658 655 . . 655 659 + BlsI GCNGC 657 656 . . 655 659 + BisI GCNGC 656 657 . . 656 659 + AciI CCGC 656 658 . . 658 662 + TauI GCSGC 661 658 . . 658 662 + BlsI GCNGC 660 659 . . 658 662 + BisI GCNGC 659 660 . . 659 662 + AciI CCGC 659 661 . . 661 666 - BsmI GAATGC 661 659 . . 674 679 + Hpy178III* TCNNGA 675 677 . . 672 676 - FokI GGATG 658 662 . . 672 676 - BseGI GGATG 671 669 . . 677 682 + McrI* CGRYCG 680 678 . . 678 687 + PshAI GACNNNNGTC 682 682 . . 679 684 + Cfr10I RCCGGY 679 683 . . 679 684 + BetI* WCCGGW 679 683 . . 679 684 + AgeI ACCGGT 679 683 . . 680 683 + HpaII CCGG 680 682 . . 685 689 + Tsp45I GTSAC 684 689 . . 685 689 + MaeIII GTNAC 684 689 . . 687 693 + TspRI CASTGNN 693 684 . . 687 692 - BtsI GCAGTG 686 684 . . 689 694 - EcoP15I CAGCAG 661 663 . . 702 707 + StyI CCWWGG 702 706 . . 702 707 + SecI* CCNNGG 702 706 . . 702 707 + AvrII CCTAGG 702 706 . . 703 706 + MaeI CTAG 703 705 . . 707 711 + TfiI GAWTC 707 710 . . 707 711 + HinfI GANTC 707 710 . . 709 713 - TspDTI ATGAA 699 697 . . 711 716 - BtgZI GCGATG 696 700 . . 716 727 - CspCI CAANNNNNGTGG 740 738 705 703 740 744 - MboII GAAGA 732 731 . . 746 749 + MaeI CTAG 746 748 . . 748 751 + SetI ASST 751 747 . . 750 753 + Csp6I GTAC 750 752 . . 750 753 + RsaI GTAC 751 751 . . 761 764 + Csp6I GTAC 761 763 . . 761 764 + RsaI GTAC 762 762 . . 788 791 + MnlI CCTC 798 797 . . 794 799 + TatI WGTACW 794 798 . . 795 798 + Csp6I GTAC 795 797 . . 795 798 + RsaI GTAC 796 796 . . 802 807 + HaeII RGCGCY 806 802 . . 803 806 + Hin6I GCGC 803 805 . . 803 806 + GlaI GCGC 804 804 . . 803 813 + MwoI GCNNNNNNNGC 809 806 . . 803 806 + HhaI GCGC 805 803 . . 808 812 + BssKI CCNGG 807 812 . . 808 812 + BseBI CCWGG 809 810 . . 808 812 + ScrFI CCNGG 809 810 . . 808 812 + EcoRII CCWGG 807 812 . . 806 811 - Eco57MI CTGRAG 791 789 . . 811 814 + CviJI RGCY 812 812 . . 806 811 - GsuI CTGGAG 791 789 . . 833 838 + Hpy178III* TCNNGA 834 836 . . 835 839 + DdeI CTNAG 835 838 . . 838 843 + XhoII RGATCY 838 842 . . 838 843 + BglII AGATCT 838 842 . . 839 842 + DpnI GATC 840 840 . . 839 842 + BstKTI GATC 841 839 . . 835 839 - BspCNI CTCAG 827 825 . . 835 839 - BseMII CTCAG 826 824 . . 839 842 + MboI GATC 838 842 . . 858 861 + MaeI CTAG 858 860 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 220 641 # # AciI: # [CCGC] # 3 22 73 202 228 333 # # AgeI: # [ACCGGT] # 182 679 # # AjuI: # [GAANNNNNNNTTGG] # 32 217 612 # # AloI: # [GAACNNNNNNTCC] # 32 396 433 # # AluI: # [AGCT] # 351 510 # # ApoI: # [RAATTY] # 59 128 204 216 254 # # AvrII: # [CCTAGG] # 159 702 # # BbvI: # [GCAGC] # 345 516 # # BbvII*: # [GAAGAC] # 185 676 # # BccI: # [CCATC] # 313 548 # # BcgI: # [CGANNNNNNTGC] # 27 34 800 # # BetI*: # [WCCGGW] # 182 292 387 # # BglII: # [AGATCT] # 23 838 # # BisI: # [GCNGC] # 3 23 202 297 336 # # BlsI: # [GCNGC] # 3 24 201 297 336 # # BsaAI: # [YACGTR] # 60 801 # # BsaBI: # [GATNNNNATC] # 373 488 # # BseBI: # [CCWGG] # 52 170 639 # # BseGI: # [GGATG] # 190 311 360 # # BseMII: # [CTCAG] # 35 317 509 # # BsiYI*: # [CCNNNNNNNGG] # 50 90 139 582 # # BsmI: # [GAATGC] # 200 661 # # BspCNI: # [CTCAG] # 34 319 508 # # BssKI: # [CCNGG] # 54 170 637 # # BstKTI: # [GATC] # 20 107 119 266 349 # # BtgZI: # [GCGATG] # 165 696 # # BtsI: # [GCAGTG] # 175 686 # # Cfr10I: # [RCCGGY] # 182 679 # # ClaI: # [ATCGAT] # 146 715 # # Csp6I: # [GTAC] # 11 34 66 750 # # CspCI: # [CAANNNNNGTGG] # 35 121 705 # # CviAII: # [CATG] # 76 232 245 308 # # CviJI: # [RGCY] # 5 25 25 26 27 30 # 49 63 128 232 251 # # CviRI*: # [TGCA] # 3 35 96 331 396 # # DdeI: # [CTNAG] # 26 340 495 # # DpnI: # [GATC] # 21 107 118 266 349 # # Eco57MI: # [CTGRAG] # 70 791 # # EcoP15I: # [CAGCAG] # 200 661 # # EcoRI: # [GAATTC] # 216 645 # # EcoRII: # [CCWGG] # 54 170 637 # # FalI: # [AAGNNNNNCTT] # 0 0 32 399 430 # # FatI: # [CATG] # 75 232 245 309 # # FokI: # [GGATG] # 203 291 367 # # GlaI: # [GCGC] # 57 804 # # GsuI: # [CTGGAG] # 70 791 # # HaeII: # [RGCGCY] # 55 806 # # HaeIII: # [GGCC] # 25 378 458 # # HgaI: # [GACGC] # 407 454 # # HhaI: # [GCGC] # 56 805 # # Hin4II*: # [CCTTC] # 243 618 # # Hin6I: # [GCGC] # 58 803 # # HindII: # [GTYRAC] # 348 513 # # HinfI: # [GANTC] # 22 52 88 143 154 194 # 208 # # HpaII: # [CCGG] # 181 292 388 # # HphI: # [GGTGA] # 173 204 234 250 # # Hpy166II: # [GTNNAC] # 221 292 348 # # Hpy178III*: # [TCNNGA] # 27 143 159 237 295 # # Hpy188I: # [TCNGA] # 161 337 363 # # MaeI: # [CTAG] # 3 43 107 112 141 167 # 288 # # MaeII: # [ACGT] # 59 802 # # MaeIII: # [GTNAC] # 51 177 633 # # MboI: # [GATC] # 23 107 116 266 349 # # MboII: # [GAAGA] # 129 132 190 410 # # McrI*: # [CGRYCG] # 181 680 # # MfeI: # [CAATTG] # 280 581 # # MlyI: # [GAGTC] # 226 635 # # MmeI: # [TCCRAC] # 184 677 # # MnlI: # [CCTC] # 38 61 63 84 183 432 # # MseI: # [TTAA] # 27 74 228 233 299 # # MwoI: # [GCNNNNNNNGC] # 52 61 748 # # NlaIII: # [CATG] # 79 232 245 305 # # NlaIV: # [GGNNCC] # 392 469 # # NmeAIII: # [GCCGAG] # 279 582 # # PleI: # [GAGTC] # 225 636 # # PshAI: # [GACNNNNGTC] # 179 682 # # RsaI: # [GTAC] # 11 34 65 751 # # ScrFI: # [CCNGG] # 52 170 639 # # SecI*: # [CCNNGG] # 26 159 676 # # SetI: # [ASST] # 11 17 53 62 78 110 # 111 123 132 164 # # SexAI: # [ACCWGGT] # 224 637 # # SfaNI: # [GCATC] # 345 516 # # StyI: # [CCWWGG] # 26 159 676 # # TaiI: # [ACGT] # 62 799 # # TaqI: # [TCGA] # 146 336 379 # # TatI: # [WGTACW] # 67 794 # # TauI: # [GCSGC] # 3 25 200 633 # # TfiI: # [GAWTC] # 52 88 143 154 208 216 # # TseI: # [GCWGC] # 358 503 # # TsoI: # [TARCCA] # 360 501 # # Tsp45I: # [GTSAC] # 51 177 633 # # Tsp4CI*: # [ACNGT] # 18 156 687 # # TspDTI: # [ATGAA] # 50 59 64 99 100 162 # 327 # # TspEI: # [AATT] # 4 21 43 59 64 92 # 162 200 216 # # TspRI: # [CASTGNN] # 168 693 # # XhoII: # [RGATCY] # 23 838 # # XmnI: # [GAANNNNTTC] # 155 706 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 861 # Reported_sequences: 1 # Reported_hitcount: 215 #---------------------------------------