Restriction Fragments for SRT1/YMR101C



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The currently selected gene/sequence is :

SRT1/YMR101C on chromosome XIII from coordinates 469476 to 468445.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.6711.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 11 15 - BsrI ACTGG 11 9 . . 10 15 - BfiI ACTGGG 5 4 . . 21 25 + Hpy188I TCNGA 23 22 . . 24 28 + TfiI GAWTC 24 27 . . 24 28 + HinfI GANTC 24 27 . . 36 39 + CviJI RGCY 37 37 . . 38 48 + BsiYI* CCNNNNNNNGG 44 41 . . 47 50 + CviJI RGCY 48 48 . . 82 87 + BtsI GCAGTG 89 87 . . 83 89 + TspRI CASTGNN 89 80 . . 99 103 + Hpy188I TCNGA 101 100 . . 127 130 + MseI TTAA 127 129 . . 129 134 + HindIII AAGCTT 129 133 . . 130 133 + SetI ASST 133 129 . . 130 133 + CviJI RGCY 131 131 . . 130 133 + AluI AGCT 131 131 . . 140 143 + FatI CATG 139 143 . . 140 143 + NlaIII CATG 143 139 . . 140 143 + CviAII CATG 140 142 . . 138 142 - TspDTI ATGAA 128 126 . . 159 163 + Hpy188I TCNGA 161 160 . . 156 160 - MboII GAAGA 148 147 . . 157 162 - Eco57MI CTGRAG 142 140 . . 157 162 - Eco57I CTGAAG 142 140 . . 163 168 + SspI AATATT 165 165 . . 181 184 + MseI TTAA 181 183 . . 190 194 + BmgT120I GGNCC 191 192 . . 190 194 + AsuI* GGNCC 190 193 . . 191 194 + CviJI RGCY 192 192 . . 191 194 + HaeIII GGCC 192 192 . . 194 200 + TspRI CASTGNN 200 191 . . 194 202 + AlwNI CAGNNNCTG 199 196 . . 193 197 - BsrI ACTGG 193 191 . . 204 209 + BspLU11I* ACATGT 204 208 . . 204 209 + NspI RCATGY 208 204 . . 204 209 + AflIII ACRYGT 204 208 . . 205 208 + FatI CATG 204 208 . . 205 208 + NlaIII CATG 208 204 . . 205 208 + CviAII CATG 205 207 . . 208 212 + BsmAI GTCTC 213 217 . . 219 222 + FatI CATG 218 222 . . 219 222 + NlaIII CATG 222 218 . . 219 222 + CviAII CATG 219 221 . . 222 226 + FokI GGATG 235 239 . . 222 226 + BseGI GGATG 228 226 . . 226 232 + BstEII GGTNACC 226 231 . . 227 231 + MaeIII GTNAC 226 231 . . 223 227 - BccI CCATC 217 218 . . 230 235 + BetI* WCCGGW 230 234 . . 231 234 + HpaII CCGG 231 233 . . 247 252 + Hpy178III* TCNNGA 248 250 . . 254 257 + CviJI RGCY 255 255 . . 251 255 - Hin4II* CCTTC 245 244 . . 259 263 - BsrI ACTGG 259 257 . . 271 274 + CviJI RGCY 272 272 . . 271 274 + HaeIII GGCC 272 272 . . 273 287 + XcmI CCANNNNNNNNNTGG 280 279 . . 273 284 + BstXI CCANNNNNNTGG 280 276 . . 274 277 + FatI CATG 273 277 . . 274 277 + NlaIII CATG 277 273 . . 274 277 + CviAII CATG 274 276 . . 275 279 + TspDTI ATGAA 290 288 . . 279 282 + SetI ASST 282 278 . . 279 282 + CviJI RGCY 280 280 . . 279 282 + AluI AGCT 280 280 . . 288 293 + Hpy166II GTNNAC 290 290 . . 288 293 + HpaI GTTAAC 290 290 . . 288 293 + HindII GTYRAC 290 290 . . 289 292 + MseI TTAA 289 291 . . 291 296 + AclI AACGTT 292 294 . . 292 295 + TaiI ACGT 295 291 . . 292 295 + SetI ASST 295 291 . . 292 295 + MaeII ACGT 292 294 . . 294 298 + MaeIII GTNAC 293 298 . . 306 310 + Tsp4CI* ACNGT 308 307 . . 316 319 + CviRI* TGCA 317 317 . . 326 331 - TaqII CACCCA 316 314 . . 344 347 + AciI CCGC 344 346 . . 343 348 - EciI GGCGGA 333 331 . . 350 355 + EcoT22I ATGCAT 354 350 . . 351 354 + CviRI* TGCA 352 352 . . 366 371 + ApoI RAATTY 366 370 . . 367 370 + TspEI AATT 366 370 . . 371 374 + MseI TTAA 371 373 . . 385 389 + MboII GAAGA 397 396 . . 403 407 + TspDTI ATGAA 418 416 . . 405 410 + ApoI RAATTY 405 409 . . 406 409 + TspEI AATT 405 409 . . 411 416 + Hpy166II GTNNAC 413 413 . . 415 419 + Tsp4CI* ACNGT 417 416 . . 421 426 + HindIII AAGCTT 421 425 . . 422 425 + SetI ASST 425 421 . . 422 425 + CviJI RGCY 423 423 . . 422 425 + AluI AGCT 423 423 . . 428 432 + TspDTI ATGAA 443 441 . . 430 435 + EcoRI GAATTC 430 434 . . 430 435 + ApoI RAATTY 430 434 . . 431 434 + TspEI AATT 430 434 . . 436 446 + MwoI GCNNNNNNNGC 442 439 . . 444 447 + CviJI RGCY 445 445 . . 446 451 + StyI CCWWGG 446 450 . . 446 451 + SecI* CCNNGG 446 450 . . 457 467 + FalI AAGNNNNNCTT 448 443 480 475 459 464 + XhoII RGATCY 459 463 . . 459 464 + NlaIV GGNNCC 461 461 . . 459 464 + BamHI GGATCC 459 463 . . 459 463 + BinI* GGATC 467 468 . . 460 463 + DpnI GATC 461 461 . . 460 463 + BstKTI GATC 462 460 . . 460 463 + MboI GATC 459 463 . . 463 473 + BsiYI* CCNNNNNNNGG 469 466 . . 460 464 - BinI* GGATC 454 455 . . 457 467 - FalI AAGNNNNNCTT 480 475 448 443 470 474 + TspGWI ACGGA 485 483 . . 472 477 + XhoII RGATCY 472 476 . . 472 476 + BinI* GGATC 480 481 . . 473 476 + DpnI GATC 474 474 . . 473 476 + BstKTI GATC 475 473 . . 473 476 + MboI GATC 472 476 . . 492 495 + SetI ASST 495 491 . . 493 497 + HphI GGTGA 505 504 . . 495 500 + BclI TGATCA 495 499 . . 496 499 + DpnI GATC 497 497 . . 496 499 + BstKTI GATC 498 496 . . 496 499 + MboI GATC 495 499 . . 513 518 + Hpy178III* TCNNGA 514 516 . . 512 517 - Eco57MI CTGRAG 497 495 . . 512 517 - GsuI CTGGAG 497 495 . . 531 534 + TspEI AATT 530 534 . . 533 536 + MseI TTAA 533 535 . . 544 548 + MboII GAAGA 556 555 . . 558 562 + FokI GGATG 571 575 . . 558 562 + BseGI GGATG 564 562 . . 559 563 - BccI CCATC 553 554 . . 563 567 - BsmAI GTCTC 557 561 . . 563 568 - Esp3I CGTCTC 557 561 . . 601 606 + Hpy178III* TCNNGA 602 604 . . 615 619 + MaeIII GTNAC 614 619 . . 627 632 + Hpy178III* TCNNGA 628 630 . . 631 635 + TfiI GAWTC 631 634 . . 631 635 + HinfI GANTC 631 634 . . 640 644 + MboII GAAGA 652 651 . . 640 645 + BbvII* GAAGAC 647 651 . . 664 669 + StyI CCWWGG 664 668 . . 664 669 + SecI* CCNNGG 664 668 . . 661 665 - HphI GGTGA 653 652 . . 670 675 + VspI ATTAAT 671 673 . . 671 674 + MseI TTAA 671 673 . . 682 687 + ApoI RAATTY 682 686 . . 683 686 + TspEI AATT 682 686 . . 698 703 + TatI WGTACW 698 702 . . 698 703 + Bsp1407I TGTACA 698 702 . . 699 702 + Csp6I GTAC 699 701 . . 699 702 + RsaI GTAC 700 700 . . 702 705 + FatI CATG 701 705 . . 702 705 + NlaIII CATG 705 701 . . 702 705 + CviAII CATG 702 704 . . 728 731 + TspEI AATT 727 731 . . 732 737 + VspI ATTAAT 733 735 . . 733 736 + MseI TTAA 733 735 . . 737 741 + Hpy188I TCNGA 739 738 . . 750 760 + MwoI GCNNNNNNNGC 756 753 . . 758 761 + CviJI RGCY 759 759 . . 756 759 - MnlI CCTC 749 748 . . 762 766 + DdeI CTNAG 762 765 . . 762 766 + BspCNI CTCAG 775 773 . . 762 766 + BseMII CTCAG 776 774 . . 763 767 + Hpy188I TCNGA 765 764 . . 783 788 + TatI WGTACW 783 787 . . 784 787 + Csp6I GTAC 784 786 . . 784 787 + RsaI GTAC 785 785 . . 787 796 + MslI CAYNNNNRTG 791 791 . . 787 790 + FatI CATG 786 790 . . 787 790 + NlaIII CATG 790 786 . . 787 790 + CviAII CATG 787 789 . . 788 792 + TspDTI ATGAA 803 801 . . 805 810 + ApoI RAATTY 805 809 . . 806 809 + TspEI AATT 805 809 . . 817 820 + TaiI ACGT 820 816 . . 817 820 + SetI ASST 820 816 . . 817 820 + MaeII ACGT 817 819 . . 823 828 + BalI TGGCCA 825 825 . . 823 828 + CfrI YGGCCR 823 827 . . 824 827 + CviJI RGCY 825 825 . . 824 827 + HaeIII GGCC 825 825 . . 828 833 + ApoI RAATTY 828 832 . . 829 832 + TspEI AATT 828 832 . . 835 838 + SetI ASST 838 834 . . 835 838 + CviJI RGCY 836 836 . . 835 838 + AluI AGCT 836 836 . . 849 852 + Csp6I GTAC 849 851 . . 849 852 + RsaI GTAC 850 850 . . 851 854 + SetI ASST 854 850 . . 854 865 + BdaI TGANNNNNNTCA 843 841 877 875 858 862 + TfiI GAWTC 858 861 . . 858 862 + HinfI GANTC 858 861 . . 854 865 - BdaI TGANNNNNNTCA 877 875 843 841 869 873 + BmgT120I GGNCC 870 871 . . 869 873 + AvaII GGWCC 869 872 . . 869 873 + AsuI* GGNCC 869 872 . . 872 876 + Hin4II* CCTTC 882 881 . . 900 911 + BdaI TGANNNNNNTCA 889 887 923 921 903 907 + MboII GAAGA 915 914 . . 906 910 + TfiI GAWTC 906 909 . . 906 910 + HinfI GANTC 906 909 . . 900 911 - BdaI TGANNNNNNTCA 923 921 889 887 931 934 + FatI CATG 930 934 . . 931 934 + NlaIII CATG 934 930 . . 931 934 + CviAII CATG 931 933 . . 932 936 + TspDTI ATGAA 947 945 . . 934 943 + XmnI GAANNNNTTC 938 938 . . 936 946 + FalI AAGNNNNNCTT 927 922 959 954 943 947 + Hin4II* CCTTC 953 952 . . 936 946 - FalI AAGNNNNNCTT 959 954 927 922 948 953 + SspI AATATT 950 950 . . 952 957 + AhaIII* TTTAAA 954 954 . . 953 956 + MseI TTAA 953 955 . . 969 972 + SetI ASST 972 968 . . 969 972 + CviJI RGCY 970 970 . . 969 972 + AluI AGCT 970 970 . . 980 985 + TatI WGTACW 980 984 . . 980 985 + Bsp1407I TGTACA 980 984 . . 981 984 + Csp6I GTAC 981 983 . . 981 984 + RsaI GTAC 982 982 . . 1015 1019 + MaeIII GTNAC 1014 1019 . . #--------------------------------------- # # # AciI: # [CCGC] # 344 688 # # AclI: # [AACGTT] # 292 740 # # AflIII: # [ACRYGT] # 204 828 # # AhaIII*: # [TTTAAA] # 78 954 # # AluI: # [AGCT] # 62 131 134 143 149 413 # # AlwNI: # [CAGNNNCTG] # 199 833 # # ApoI: # [RAATTY] # 23 25 39 123 204 252 # 366 # # AsuI*: # [GGNCC] # 163 190 679 # # AvaII: # [GGWCC] # 163 869 # # BalI: # [TGGCCA] # 207 825 # # BamHI: # [GGATCC] # 459 573 # # BbvII*: # [GAAGAC] # 385 647 # # BccI: # [CCATC] # 217 336 479 # # BclI: # [TGATCA] # 495 537 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 12 34 # 34 109 843 # # BetI*: # [WCCGGW] # 230 802 # # BfiI: # [ACTGGG] # 5 1027 # # BinI*: # [GGATC] # 13 13 454 552 # # BmgT120I: # [GGNCC] # 162 191 679 # # BseGI: # [GGATG] # 228 336 468 # # BseMII: # [CTCAG] # 256 776 # # BsiYI*: # [CCNNNNNNNGG] # 44 425 563 # # BsmAI: # [GTCTC] # 213 344 475 # # Bsp1407I: # [TGTACA] # 52 282 698 # # BspCNI: # [CTCAG] # 257 775 # # BspLU11I*: # [ACATGT] # 204 828 # # BsrI: # [ACTGG] # 11 66 182 773 # # BstEII: # [GGTNACC] # 226 806 # # BstKTI: # [GATC] # 13 23 462 534 # # BstXI: # [CCANNNNNNTGG] # 280 752 # # BtsI: # [GCAGTG] # 89 943 # # CfrI: # [YGGCCR] # 209 823 # # Csp6I: # [GTAC] # 51 65 85 132 699 # # CviAII: # [CATG] # 14 55 65 85 101 140 # 144 428 # # CviJI: # [RGCY] # 8 11 11 17 22 37 # 61 62 63 66 83 134 # 143 314 # # CviRI*: # [TGCA] # 35 317 680 # # DdeI: # [CTNAG] # 270 762 # # DpnI: # [GATC] # 13 23 461 535 # # EciI: # [GGCGGA] # 333 699 # # Eco57I: # [CTGAAG] # 142 890 # # Eco57MI: # [CTGRAG] # 142 355 535 # # EcoRI: # [GAATTC] # 430 602 # # EcoT22I: # [ATGCAT] # 354 678 # # Esp3I: # [CGTCTC] # 475 557 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 73 447 448 # # FatI: # [CATG] # 14 55 65 85 102 139 # 144 428 # # FokI: # [GGATG] # 235 336 461 # # GsuI: # [CTGGAG] # 497 535 # # HaeIII: # [GGCC] # 80 192 207 553 # # Hin4II*: # [CCTTC] # 71 79 245 637 # # HindII: # [GTYRAC] # 290 742 # # HindIII: # [AAGCTT] # 129 292 611 # # HinfI: # [GANTC] # 24 48 126 227 607 # # HpaI: # [GTTAAC] # 290 742 # # HpaII: # [CCGG] # 231 801 # # HphI: # [GGTGA] # 148 379 505 # # Hpy166II: # [GTNNAC] # 123 290 619 # # Hpy178III*: # [TCNNGA] # 26 88 248 266 404 # # Hpy188I: # [TCNGA] # 23 26 60 78 267 578 # # MaeII: # [ACGT] # 215 292 525 # # MaeIII: # [GTNAC] # 18 67 226 321 400 # # MboI: # [GATC] # 13 23 459 537 # # MboII: # [GAAGA] # 96 117 148 159 249 263 # # MnlI: # [CCTC] # 283 749 # # MseI: # [TTAA] # 54 62 79 82 108 127 # 138 162 220 # # MslI: # [CAYNNNNRTG] # 241 791 # # MwoI: # [GCNNNNNNNGC] # 276 314 442 # # NlaIII: # [CATG] # 14 55 65 85 98 143 # 144 428 # # NlaIV: # [GGNNCC] # 461 571 # # NspI: # [RCATGY] # 208 824 # # RsaI: # [GTAC] # 50 65 85 132 700 # # SecI*: # [CCNNGG] # 218 368 446 # # SetI: # [ASST] # 13 16 18 60 70 118 # 130 133 149 325 # # SspI: # [AATATT] # 82 165 785 # # StyI: # [CCWWGG] # 218 368 446 # # TaiI: # [ACGT] # 212 295 525 # # TaqII: # [CACCCA] # 316 716 # # TatI: # [WGTACW] # 52 85 197 698 # # TfiI: # [GAWTC] # 24 48 126 227 607 # # Tsp4CI*: # [ACNGT] # 109 308 615 # # TspDTI: # [ATGAA] # 16 25 85 112 128 144 # 162 360 # # TspEI: # [AATT] # 23 25 39 45 78 100 # 152 204 366 # # TspGWI: # [ACGGA] # 485 547 # # TspRI: # [CASTGNN] # 89 111 832 # # VspI: # [ATTAAT] # 62 299 671 # # XcmI: # [CCANNNNNNNNNTGG] # 280 752 # # XhoII: # [RGATCY] # 13 459 560 # # XmnI: # [GAANNNNTTC] # 94 938 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1032 # Reported_sequences: 1 # Reported_hitcount: 226 #---------------------------------------