Restriction Fragments for CSM3/YMR048W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

CSM3/YMR048W on chromosome XIII from coordinates 366981 to 367934.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.6906.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + BinI* GGATC 11 12 . . 4 7 + DpnI GATC 5 5 . . 4 7 + BstKTI GATC 6 4 . . 4 7 + MboI GATC 3 7 . . 6 11 + Hpy178III* TCNNGA 7 9 . . 17 21 + Tsp4CI* ACNGT 19 18 . . 28 31 + MaeI CTAG 28 30 . . 30 33 + SetI ASST 33 29 . . 40 44 + HinfI GANTC 40 43 . . 43 47 + Hpy188I TCNGA 45 44 . . 40 44 - PleI GAGTC 34 35 . . 40 44 - MlyI GAGTC 34 34 . . 67 71 + Tsp4CI* ACNGT 69 68 . . 70 73 + Csp6I GTAC 70 72 . . 70 73 + RsaI GTAC 71 71 . . 79 82 + CviJI RGCY 80 80 . . 84 88 + FokI GGATG 97 101 . . 84 88 + BseGI GGATG 90 88 . . 85 89 - BccI CCATC 79 80 . . 100 103 + DpnI GATC 101 101 . . 100 103 + BstKTI GATC 102 100 . . 100 103 + MboI GATC 99 103 . . 100 104 - BinI* GGATC 94 95 . . 108 113 - BsrDI GCAATG 107 105 . . 114 119 + XhoII RGATCY 114 118 . . 114 119 + NlaIV GGNNCC 116 116 . . 114 119 + BamHI GGATCC 114 118 . . 114 118 + BinI* GGATC 122 123 . . 115 118 + DpnI GATC 116 116 . . 115 118 + BstKTI GATC 117 115 . . 115 118 + MboI GATC 114 118 . . 112 115 - AciI CCGC 112 114 . . 117 122 + MmeI TCCRAC 142 140 . . 115 119 - BinI* GGATC 109 110 . . 122 125 + AciI CCGC 122 124 . . 124 134 + MwoI GCNNNNNNNGC 130 127 . . 126 129 + TspEI AATT 125 129 . . 132 135 + SetI ASST 135 131 . . 132 135 + CviJI RGCY 133 133 . . 132 135 + AluI AGCT 133 133 . . 134 137 + MaeI CTAG 134 136 . . 141 151 + BplI GAGNNNNNCTC 132 127 164 159 145 150 + StuI AGGCCT 147 147 . . 146 149 + CviJI RGCY 147 147 . . 146 149 + HaeIII GGCC 147 147 . . 143 147 - Hin4II* CCTTC 137 136 . . 148 151 + MnlI CCTC 158 157 . . 149 154 + SmlI CTYRAG 149 153 . . 141 151 - BplI GAGNNNNNCTC 164 159 132 127 149 154 - Bce83I* CTTGAG 134 132 . . 158 161 + TspEI AATT 157 161 . . 160 163 + MseI TTAA 160 162 . . 165 168 + CviJI RGCY 166 166 . . 178 182 + DdeI CTNAG 178 181 . . 178 182 + BspCNI CTCAG 191 189 . . 178 182 + BseMII CTCAG 192 190 . . 180 186 + TspRI CASTGNN 186 177 . . 189 192 + SetI ASST 192 188 . . 191 196 + Hpy166II GTNNAC 193 193 . . 197 202 + NdeI CATATG 198 200 . . 213 216 + CviRI* TGCA 214 214 . . 224 229 - BciVI GTATCC 218 217 . . 230 235 + ApoI RAATTY 230 234 . . 231 234 + TspEI AATT 230 234 . . 236 239 + MnlI CCTC 246 245 . . 251 256 + NdeI CATATG 252 254 . . 256 266 + Hin4I GAYNNNNNVTC 247 242 279 274 256 266 - Hin4I GAYNNNNNVTC 279 274 247 242 268 273 + SspI AATATT 270 270 . . 285 295 + BsiYI* CCNNNNNNNGG 291 288 . . 287 290 + SetI ASST 290 286 . . 287 290 + CviJI RGCY 288 288 . . 287 290 + AluI AGCT 288 288 . . 293 298 + SduI GDGCHC 297 293 . . 293 298 + BseSI GKGCMC 297 293 . . 298 301 + FatI CATG 297 301 . . 298 301 + NlaIII CATG 301 297 . . 298 301 + CviAII CATG 298 300 . . 299 303 + TspDTI ATGAA 314 312 . . 301 310 + XmnI GAANNNNTTC 305 305 . . 302 305 + TspEI AATT 301 305 . . 311 316 + StyI CCWWGG 311 315 . . 311 316 + SecI* CCNNGG 311 315 . . 319 324 + ApoI RAATTY 319 323 . . 320 323 + TspEI AATT 319 323 . . 323 326 + MseI TTAA 323 325 . . 334 338 + TspDTI ATGAA 349 347 . . 336 340 + MboII GAAGA 348 347 . . 338 343 + XhoII RGATCY 338 342 . . 338 343 + BglII AGATCT 338 342 . . 339 342 + DpnI GATC 340 340 . . 339 342 + BstKTI GATC 341 339 . . 339 342 + MboI GATC 338 342 . . 349 353 + Tsp4CI* ACNGT 351 350 . . 354 357 + SetI ASST 357 353 . . 363 368 + XhoII RGATCY 363 367 . . 364 367 + DpnI GATC 365 365 . . 364 367 + BstKTI GATC 366 364 . . 364 367 + MboI GATC 363 367 . . 364 368 - BinI* GGATC 358 359 . . 367 371 - BsrI ACTGG 367 365 . . 373 377 + DdeI CTNAG 373 376 . . 386 390 + PleI GAGTC 394 395 . . 386 390 + MlyI GAGTC 395 395 . . 386 390 + HinfI GANTC 386 389 . . 391 394 + CviJI RGCY 392 392 . . 393 403 + BsiYI* CCNNNNNNNGG 399 396 . . 401 405 + BslFI GGGAC 415 419 . . 408 412 - BccI CCATC 402 403 . . 414 417 + FatI CATG 413 417 . . 414 417 + NlaIII CATG 417 413 . . 414 417 + CviAII CATG 414 416 . . 422 425 + TaqI TCGA 422 424 . . 413 424 - BcgI CGANNNNNNTGC 436 434 402 400 442 446 + BsrI ACTGG 447 445 . . 442 447 + BfiI ACTGGG 452 451 . . 439 443 - BsmAI GTCTC 433 437 . . 452 455 + SetI ASST 455 451 . . 453 457 + BssKI CCNGG 452 457 . . 453 457 + BseBI CCWGG 454 455 . . 453 457 + ScrFI CCNGG 454 455 . . 453 457 + EcoRII CCWGG 452 457 . . 468 471 + SetI ASST 471 467 . . 466 470 - HphI GGTGA 458 457 . . 478 482 + MboII GAAGA 490 489 . . 483 488 + BspLU11I* ACATGT 483 487 . . 483 488 + NspI RCATGY 487 483 . . 483 488 + AflIII ACRYGT 483 487 . . 484 487 + FatI CATG 483 487 . . 484 487 + NlaIII CATG 487 483 . . 484 487 + CviAII CATG 484 486 . . 487 491 + Tsp45I GTSAC 486 491 . . 487 491 + MaeIII GTNAC 486 491 . . 494 499 + NspBII* CMGCKG 496 496 . . 494 499 + SacII CCGCGG 497 495 . . 494 499 + DsaI* CCRYGG 494 498 . . 494 499 + SecI* CCNNGG 494 498 . . 494 497 + AciI CCGC 494 496 . . 495 498 + FnuDII* CGCG 496 496 . . 499 503 + MboII GAAGA 511 510 . . 496 499 - AciI CCGC 496 498 . . 499 504 - Ksp632I* CTCTTC 494 497 . . 505 510 + StuI AGGCCT 507 507 . . 506 509 + CviJI RGCY 507 507 . . 506 509 + HaeIII GGCC 507 507 . . 504 507 - MnlI CCTC 497 496 . . 528 533 + MstI* TGCGCA 530 530 . . 529 532 + Hin6I GCGC 529 531 . . 529 532 + GlaI GCGC 530 530 . . 529 532 + HhaI GCGC 531 529 . . 547 552 + Hpy166II GTNNAC 549 549 . . 557 560 + TaqI TCGA 557 559 . . 574 578 + MboII GAAGA 586 585 . . 583 588 + EcoRV GATATC 585 585 . . 583 592 + BsaBI GATNNNNATC 587 587 . . 593 596 + SetI ASST 596 592 . . 591 595 - HphI GGTGA 583 582 . . 611 615 + MboII GAAGA 623 622 . . 611 616 - Ksp632I* CTCTTC 606 609 . . 614 617 - MnlI CCTC 607 606 . . 633 636 + TaiI ACGT 636 632 . . 633 636 + SetI ASST 636 632 . . 633 636 + MaeII ACGT 633 635 . . 644 648 + TspDTI ATGAA 659 657 . . 649 653 + Tsp4CI* ACNGT 651 650 . . 652 655 + Csp6I GTAC 652 654 . . 652 655 + RsaI GTAC 653 653 . . 656 659 + MseI TTAA 656 658 . . 661 666 + AclI AACGTT 662 664 . . 662 665 + TaiI ACGT 665 661 . . 662 665 + SetI ASST 665 661 . . 662 665 + MaeII ACGT 662 664 . . 669 673 + TauI GCSGC 672 669 . . 669 673 + BlsI GCNGC 671 670 . . 669 673 + BisI GCNGC 670 671 . . 670 673 + AciI CCGC 670 672 . . 673 679 + SauI* CCTNAGG 674 677 . . 674 678 + DdeI CTNAG 674 677 . . 679 683 + MboII GAAGA 691 690 . . 687 691 + FokI GGATG 700 704 . . 687 691 + BseGI GGATG 693 691 . . 689 694 + EcoT22I ATGCAT 693 689 . . 690 693 + CviRI* TGCA 691 691 . . 688 692 - SfaNI GCATC 678 682 . . 698 709 + BdaI TGANNNNNNTCA 687 685 721 719 699 703 + MboII GAAGA 711 710 . . 699 704 + BbvII* GAAGAC 706 710 . . 699 708 + XmnI GAANNNNTTC 703 703 . . 698 709 - BdaI TGANNNNNNTCA 721 719 687 685 712 715 + Csp6I GTAC 712 714 . . 712 715 + RsaI GTAC 713 713 . . 717 723 + DraII RGGNCCY 718 721 . . 718 723 + SduI GDGCHC 722 718 . . 718 723 + Bsp120I GGGCCC 718 722 . . 718 723 + NlaIV GGNNCC 720 720 . . 718 722 + BmgT120I GGNCC 719 720 . . 718 723 + HgiJII* GRGCYC 722 718 . . 718 723 + BseSI GKGCMC 722 718 . . 718 722 + AsuI* GGNCC 718 721 . . 718 723 + ApaI GGGCCC 722 718 . . 719 722 + CviJI RGCY 720 720 . . 719 722 + HaeIII GGCC 720 720 . . 719 723 + BmgT120I GGNCC 720 721 . . 719 723 + AsuI* GGNCC 719 722 . . 727 730 + CviJI RGCY 728 728 . . 727 730 + HaeIII GGCC 728 728 . . 733 737 + MboII GAAGA 745 744 . . 744 748 + MboII GAAGA 756 755 . . 745 755 + FalI AAGNNNNNCTT 736 731 768 763 749 752 + SetI ASST 752 748 . . 749 752 + CviJI RGCY 750 750 . . 749 752 + AluI AGCT 750 750 . . 745 755 - FalI AAGNNNNNCTT 768 763 736 731 760 764 + FokI GGATG 773 777 . . 760 764 + BseGI GGATG 766 764 . . 764 767 + CviJI RGCY 765 765 . . 765 775 + MwoI GCNNNNNNNGC 771 768 . . 761 765 - BccI CCATC 755 756 . . 766 769 + MaeI CTAG 766 768 . . 763 768 - TsoI TARCCA 753 751 . . 770 777 + FspAI RTGCGCAY 773 773 . . 771 776 + MstI* TGCGCA 773 773 . . 772 775 + Hin6I GCGC 772 774 . . 772 775 + GlaI GCGC 773 773 . . 772 775 + HhaI GCGC 774 772 . . 769 773 - SfaNI GCATC 759 763 . . 793 796 + CviJI RGCY 794 794 . . 803 808 + Eco57MI CTGRAG 824 822 . . 803 808 + Eco57I CTGAAG 824 822 . . 805 809 + MboII GAAGA 817 816 . . 808 812 + MboII GAAGA 820 819 . . 808 813 + BbvII* GAAGAC 815 819 . . 808 817 + XmnI GAANNNNTTC 812 812 . . 812 815 + TaiI ACGT 815 811 . . 812 815 + SetI ASST 815 811 . . 812 815 + MaeII ACGT 812 814 . . 822 826 + TfiI GAWTC 822 825 . . 822 826 + HinfI GANTC 822 825 . . 835 839 + MboII GAAGA 847 846 . . 835 840 - Ksp632I* CTCTTC 830 833 . . 847 851 + TspDTI ATGAA 862 860 . . 861 865 + BslFI GGGAC 875 879 . . 859 862 - MnlI CCTC 852 851 . . 889 893 + BssKI CCNGG 888 893 . . 889 893 + BseBI CCWGG 890 891 . . 889 894 + SecI* CCNNGG 889 893 . . 889 893 + ScrFI CCNGG 890 891 . . 889 893 + EcoRII CCWGG 888 893 . . 913 916 + DpnI GATC 914 914 . . 913 916 + BstKTI GATC 915 913 . . 913 916 + MboI GATC 912 916 . . 915 920 + Hpy178III* TCNNGA 916 918 . . 927 931 + FokI GGATG 940 944 . . 927 931 + BseGI GGATG 933 931 . . 924 929 - MmeI TCCRAC 905 903 . . 928 932 - SfaNI GCATC 918 922 . . 934 938 + TspDTI ATGAA 949 947 . . 936 940 - Hin4II* CCTTC 930 929 . . 946 949 + CviJI RGCY 947 947 . . #--------------------------------------- # # # AciI: # [CCGC] # 2 10 112 174 284 372 # # AclI: # [AACGTT] # 292 662 # # AflIII: # [ACRYGT] # 471 483 # # AluI: # [AGCT] # 133 155 204 462 # # ApaI: # [GGGCCC] # 232 722 # # ApoI: # [RAATTY] # 89 230 635 # # AsuI*: # [GGNCC] # 1 235 718 # # BamHI: # [GGATCC] # 114 840 # # BbvII*: # [GAAGAC] # 109 139 706 # # BccI: # [CCATC] # 79 199 323 353 # # Bce83I*: # [CTTGAG] # 134 820 # # BcgI: # [CGANNNNNNTGC] # 34 402 518 # # BciVI: # [GTATCC] # 218 736 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 233 687 # # BfiI: # [ACTGGG] # 452 502 # # BglII: # [AGATCT] # 338 616 # # BinI*: # [GGATC] # 11 13 15 83 236 596 # # BisI: # [GCNGC] # 284 670 # # BlsI: # [GCNGC] # 283 671 # # BmgT120I: # [GGNCC] # 1 234 719 # # BplI: # [GAGNNNNNCTC] # 0 0 32 132 790 # # BsaBI: # [GATNNNNATC] # 367 587 # # BseBI: # [CCWGG] # 64 436 454 # # BseGI: # [GGATG] # 21 73 90 167 603 # # BseMII: # [CTCAG] # 192 762 # # BseSI: # [GKGCMC] # 232 297 425 # # BsiYI*: # [CCNNNNNNNGG] # 108 291 555 # # BslFI: # [GGGAC] # 79 415 460 # # BsmAI: # [GTCTC] # 433 521 # # Bsp120I: # [GGGCCC] # 236 718 # # BspCNI: # [CTCAG] # 191 763 # # BspLU11I*: # [ACATGT] # 471 483 # # BsrDI: # [GCAATG] # 107 847 # # BsrI: # [ACTGG] # 80 367 507 # # BssKI: # [CCNGG] # 66 436 452 # # BstKTI: # [GATC] # 6 15 25 39 96 224 # 549 # # Csp6I: # [GTAC] # 60 70 242 582 # # CviAII: # [CATG] # 70 116 298 470 # # CviJI: # [RGCY] # 7 8 14 15 19 22 # 29 53 80 104 115 122 # 153 213 # # CviRI*: # [TGCA] # 214 263 477 # # DdeI: # [CTNAG] # 178 195 280 301 # # DpnI: # [GATC] # 5 15 25 40 96 224 # 549 # # DraII: # [RGGNCCY] # 236 718 # # DsaI*: # [CCRYGG] # 460 494 # # Eco57I: # [CTGAAG] # 130 824 # # Eco57MI: # [CTGRAG] # 130 824 # # EcoRII: # [CCWGG] # 66 436 452 # # EcoRV: # [GATATC] # 369 585 # # EcoT22I: # [ATGCAT] # 261 693 # # FalI: # [AAGNNNNNCTT] # 0 0 32 186 736 # # FatI: # [CATG] # 70 116 297 471 # # FnuDII*: # [CGCG] # 458 496 # # FokI: # [GGATG] # 14 73 97 167 603 # # FspAI: # [RTGCGCAY] # 181 773 # # GlaI: # [GCGC] # 181 243 530 # # HaeIII: # [GGCC] # 8 147 213 226 360 # # HgiJII*: # [GRGCYC] # 232 722 # # HhaI: # [GCGC] # 180 243 531 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 247 675 # # Hin4II*: # [CCTTC] # 24 137 793 # # Hin6I: # [GCGC] # 182 243 529 # # HinfI: # [GANTC] # 40 132 346 436 # # HphI: # [GGTGA] # 125 371 458 # # Hpy166II: # [GTNNAC] # 193 356 405 # # Hpy178III*: # [TCNNGA] # 7 38 909 # # Hpy188I: # [TCNGA] # 45 909 # # Ksp632I*: # [CTCTTC] # 112 124 224 494 # # MaeI: # [CTAG] # 28 106 188 632 # # MaeII: # [ACGT] # 29 142 150 633 # # MaeIII: # [GTNAC] # 468 486 # # MboI: # [GATC] # 3 15 25 42 96 224 # 549 # # MboII: # [GAAGA] # 3 11 20 21 27 34 # 37 61 68 75 107 142 # 348 # # MlyI: # [GAGTC] # 34 361 559 # # MmeI: # [TCCRAC] # 49 142 763 # # MnlI: # [CCTC] # 88 102 110 158 245 251 # # MseI: # [TTAA] # 160 163 298 333 # # MstI*: # [TGCGCA] # 181 243 530 # # MwoI: # [GCNNNNNNNGC] # 130 183 641 # # NdeI: # [CATATG] # 54 198 702 # # NlaIII: # [CATG] # 70 116 301 467 # # NlaIV: # [GGNNCC] # 116 234 604 # # NspBII*: # [CMGCKG] # 458 496 # # NspI: # [RCATGY] # 467 487 # # PleI: # [GAGTC] # 34 360 560 # # RsaI: # [GTAC] # 60 71 241 582 # # SacII: # [CCGCGG] # 457 497 # # SauI*: # [CCTNAGG] # 280 674 # # ScrFI: # [CCNGG] # 64 436 454 # # SduI: # [GDGCHC] # 232 297 425 # # SecI*: # [CCNNGG] # 65 183 311 395 # # SetI: # [ASST] # 16 29 33 40 57 63 # 67 87 98 98 102 125 # 139 # # SfaNI: # [GCATC] # 36 81 159 678 # # SmlI: # [CTYRAG] # 149 805 # # SspI: # [AATATT] # 270 684 # # StuI: # [AGGCCT] # 147 360 447 # # StyI: # [CCWWGG] # 311 643 # # TaiI: # [ACGT] # 29 139 150 636 # # TaqI: # [TCGA] # 135 397 422 # # TauI: # [GCSGC] # 282 672 # # TfiI: # [GAWTC] # 132 822 # # TsoI: # [TARCCA] # 201 753 # # Tsp45I: # [GTSAC] # 468 486 # # Tsp4CI*: # [ACNGT] # 19 50 282 300 303 # # TspDTI: # [ATGAA] # 5 35 87 203 310 314 # # TspEI: # [AATT] # 18 32 71 73 125 635 # # TspRI: # [CASTGNN] # 186 768 # # XhoII: # [RGATCY] # 25 114 224 591 # # XmnI: # [GAANNNNTTC] # 109 142 305 398 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 954 # Reported_sequences: 1 # Reported_hitcount: 259 #---------------------------------------