Restriction Fragments for YMLWdelta4



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YMLWdelta4 on chromosome XIII from coordinates 189752 to 190083.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.15396.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 65 69 + Tsp4CI* ACNGT 67 66 . . 71 74 + MseI TTAA 71 73 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 93 103 - Hin4I GAYNNNNNVTC 116 111 84 79 105 108 + TaqI TCGA 105 107 . . 108 111 + SetI ASST 111 107 . . 107 110 - MnlI CCTC 100 99 . . 114 117 - MnlI CCTC 107 106 . . 119 122 + SetI ASST 122 118 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + AluI AGCT 120 120 . . 121 126 + Eco57MI CTGRAG 142 140 . . 121 126 + Eco57I CTGAAG 142 140 . . 127 130 + CviRI* TGCA 128 128 . . 154 158 + TspDTI ATGAA 169 167 . . 168 172 + TspGWI ACGGA 183 181 . . 175 178 - MnlI CCTC 168 167 . . 175 185 - Hin4I GAYNNNNNVTC 198 193 166 161 188 193 + SspI AATATT 190 190 . . 210 214 + TfiI GAWTC 210 213 . . 210 214 + HinfI GANTC 210 213 . . 214 224 + BsiYI* CCNNNNNNNGG 220 217 . . 224 228 + TfiI GAWTC 224 227 . . 221 224 - MnlI CCTC 214 213 . . 224 228 + HinfI GANTC 224 227 . . 235 242 + PspXI VCTCGAGB 236 240 . . 235 238 + MnlI CCTC 245 244 . . 235 242 + AbsI CCTCGAGG 236 240 . . 236 241 + SmlI CTYRAG 236 240 . . 236 241 + XhoI CTCGAG 236 240 . . 236 241 + BmeT110I CYCGRG 237 239 . . 236 241 + AvaI CYCGRG 236 240 . . 237 240 + TaqI TCGA 237 239 . . 239 244 + BseRI GAGGAG 254 252 . . 239 242 - MnlI CCTC 232 231 . . 250 253 + MaeI CTAG 250 252 . . 262 267 + Hpy166II GTNNAC 264 264 . . 262 267 + BssNAI GTATAC 264 264 . . 262 267 + AccI GTMKAC 263 265 . . 266 269 + SetI ASST 269 265 . . 270 275 + SspI AATATT 272 272 . . 278 281 + CviJI RGCY 279 279 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . 313 316 + TspEI AATT 312 316 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 236 # # AccI: # [GTMKAC] # 69 263 # # AluI: # [AGCT] # 22 98 212 # # AvaI: # [CYCGRG] # 96 236 # # BmeT110I: # [CYCGRG] # 95 237 # # BseRI: # [GAGGAG] # 78 254 # # BsiYI*: # [CCNNNNNNNGG] # 112 220 # # BssNAI: # [GTATAC] # 68 264 # # CviJI: # [RGCY] # 22 53 98 159 # # CviRI*: # [TGCA] # 128 204 # # Eco57I: # [CTGAAG] # 142 190 # # Eco57MI: # [CTGRAG] # 142 190 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 84 134 # # HinfI: # [GANTC] # 14 37 71 210 # # Hpy166II: # [GTNNAC] # 68 264 # # MaeI: # [CTAG] # 82 250 # # MnlI: # [CCTC] # 7 13 18 46 61 87 # 100 # # MseI: # [TTAA] # 71 261 # # PspXI: # [VCTCGAGB] # 96 236 # # SetI: # [ASST] # 11 11 63 100 147 # # SmlI: # [CTYRAG] # 96 236 # # SspI: # [AATATT] # 60 82 190 # # TaqI: # [TCGA] # 95 105 132 # # TfiI: # [GAWTC] # 14 37 71 210 # # Tsp4CI*: # [ACNGT] # 67 265 # # TspDTI: # [ATGAA] # 163 169 # # TspEI: # [AATT] # 8 20 304 # # TspGWI: # [ACGGA] # 149 183 # # XhoI: # [CTCGAG] # 96 236 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 332 # Reported_sequences: 1 # Reported_hitcount: 48 #---------------------------------------