Restriction Fragments for GTR1/YML121W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

GTR1/YML121W on chromosome XIII from coordinates 26930 to 27862.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.32656.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 18 22 + MboII GAAGA 30 29 . . 30 34 + Hpy188I TCNGA 32 31 . . 36 40 + BmgT120I GGNCC 37 38 . . 36 40 + AsuI* GGNCC 36 39 . . 37 40 + CviJI RGCY 38 38 . . 37 40 + HaeIII GGCC 38 38 . . 33 37 - BccI CCATC 27 28 . . 38 48 + MwoI GCNNNNNNNGC 44 41 . . 38 43 + Cfr10I RCCGGY 38 42 . . 38 48 + BglI GCCNNNNNGGC 44 41 . . 39 42 + HpaII CCGG 39 41 . . 40 46 + RsrII CGGWCCG 41 44 . . 41 45 + BmgT120I GGNCC 42 43 . . 41 45 + AvaII GGWCC 41 44 . . 41 45 + AsuI* GGNCC 41 44 . . 44 47 + HpaII CCGG 44 46 . . 46 49 + CviJI RGCY 47 47 . . 46 51 + NlaIV GGNNCC 48 48 . . 49 54 + BetI* WCCGGW 49 53 . . 50 53 + HpaII CCGG 50 52 . . 65 76 + BdaI TGANNNNNNTCA 54 52 88 86 67 70 + SetI ASST 70 66 . . 66 69 - MnlI CCTC 59 58 . . 70 73 + TaqI TCGA 70 72 . . 72 75 + DpnI GATC 73 73 . . 72 75 + BstKTI GATC 74 72 . . 72 75 + MboI GATC 71 75 . . 65 76 - BdaI TGANNNNNNTCA 88 86 54 52 83 87 + MaeIII GTNAC 82 87 . . 92 95 + AciI CCGC 92 94 . . 104 107 + MaeI CTAG 104 106 . . 115 120 + NlaIV GGNNCC 117 117 . . 115 120 + HgiCI* GGYRCC 115 119 . . 107 117 - BsaXI ACNNNNNCTCC 129 126 99 96 107 118 - TstI CACNNNNNNTCC 131 126 99 94 113 118 - TaqII CACCCA 103 101 . . 133 138 + SduI GDGCHC 137 133 . . 133 138 + HgiAI* GWGCWC 137 133 . . 165 169 + HinfI GANTC 165 168 . . 165 175 + Hin4I GAYNNNNNVTC 156 151 188 183 165 169 - PleI GAGTC 159 160 . . 165 169 - MlyI GAGTC 159 159 . . 165 175 - Hin4I GAYNNNNNVTC 188 183 156 151 179 183 + BslFI GGGAC 193 197 . . 182 186 + Tsp4CI* ACNGT 184 183 . . 197 200 + TaiI ACGT 200 196 . . 197 200 + SetI ASST 200 196 . . 197 200 + MaeII ACGT 197 199 . . 197 202 + AflIII ACRYGT 197 201 . . 196 201 - BtrI CACGTC 198 198 . . 210 219 + XmnI GAANNNNTTC 214 214 . . 211 214 + TspEI AATT 210 214 . . 218 222 - HphI GGTGA 210 209 . . 242 247 + Hpy178III* TCNNGA 243 245 . . 250 255 + BsgI GTGCAG 271 269 . . 246 250 - BccI CCATC 240 241 . . 251 254 + CviRI* TGCA 252 252 . . 254 257 + SetI ASST 257 253 . . 252 257 - BspMI ACCTGC 243 247 . . 252 258 - AarI CACCTGC 243 247 . . 259 262 + MseI TTAA 259 261 . . 261 264 + TspEI AATT 260 264 . . 266 269 + TaiI ACGT 269 265 . . 266 269 + SetI ASST 269 265 . . 266 269 + MaeII ACGT 266 268 . . 274 284 + Hin4I GAYNNNNNVTC 265 260 297 292 280 284 + PleI GAGTC 288 289 . . 280 284 + MlyI GAGTC 289 289 . . 280 284 + HinfI GANTC 280 283 . . 282 287 + Hpy166II GTNNAC 284 284 . . 282 287 + HindII GTYRAC 284 284 . . 274 284 - Hin4I GAYNNNNNVTC 297 292 265 260 287 292 + Eco57MI CTGRAG 308 306 . . 287 292 + Eco57I CTGAAG 308 306 . . 295 300 + SmlI CTYRAG 295 299 . . 295 300 - Bce83I* CTTGAG 280 278 . . 309 314 + SspI AATATT 311 311 . . 315 318 + CviRI* TGCA 316 316 . . 320 325 + HindIII AAGCTT 320 324 . . 321 324 + SetI ASST 324 320 . . 321 324 + CviJI RGCY 322 322 . . 321 324 + AluI AGCT 322 322 . . 332 335 + TspEI AATT 331 335 . . 334 337 + MseI TTAA 334 336 . . 341 346 + TatI WGTACW 341 345 . . 341 346 + ScaI AGTACT 343 343 . . 342 345 + Csp6I GTAC 342 344 . . 342 345 + RsaI GTAC 343 343 . . 348 353 + Hpy178III* TCNNGA 349 351 . . 351 355 + Hpy99I CGWCG 355 350 . . 352 356 + HgaI GACGC 361 366 . . 352 357 + AcyI GRCGYC 353 355 . . 359 364 + ApoI RAATTY 359 363 . . 360 363 + TspEI AATT 359 363 . . 369 373 - MboII GAAGA 361 360 . . 374 377 + CviRI* TGCA 375 375 . . 384 389 + XhoII RGATCY 384 388 . . 384 388 + BinI* GGATC 392 393 . . 385 388 + DpnI GATC 386 386 . . 385 388 + BstKTI GATC 387 385 . . 385 388 + MboI GATC 384 388 . . 381 385 - BccI CCATC 375 376 . . 396 401 - MmeI TCCRAC 377 375 . . 409 414 + BseRI GAGGAG 424 422 . . 409 412 - MnlI CCTC 402 401 . . 413 416 + SetI ASST 416 412 . . 413 416 + CviJI RGCY 414 414 . . 413 416 + AluI AGCT 414 414 . . 409 420 - PpiI GAACNNNNNCTC 432 427 400 395 425 430 + Hpy178III* TCNNGA 426 428 . . 425 430 + BspHI TCATGA 425 429 . . 426 429 + FatI CATG 425 429 . . 426 429 + NlaIII CATG 429 425 . . 426 429 + CviAII CATG 426 428 . . 430 434 + TspDTI ATGAA 445 443 . . 437 440 + SetI ASST 440 436 . . 439 443 + DdeI CTNAG 439 442 . . 439 443 - BspCNI CTCAG 431 429 . . 439 443 - BseMII CTCAG 430 428 . . 448 451 + TaiI ACGT 451 447 . . 448 451 + SetI ASST 451 447 . . 448 451 + MaeII ACGT 448 450 . . 454 458 + Hpy188I TCNGA 456 455 . . 451 455 - MboII GAAGA 443 442 . . 450 455 - BbvII* GAAGAC 443 447 . . 457 462 + ApoI RAATTY 457 461 . . 458 461 + TspEI AATT 457 461 . . 474 478 + Hpy188I TCNGA 476 475 . . 480 483 + SetI ASST 483 479 . . 493 496 + TaqI TCGA 493 495 . . 501 505 + FokI GGATG 514 518 . . 501 505 + BseGI GGATG 507 505 . . 521 524 + FatI CATG 520 524 . . 521 524 + NlaIII CATG 524 520 . . 521 524 + CviAII CATG 521 523 . . 539 544 + Cac8I GCNNGC 541 541 . . 559 564 + MmeI TCCRAC 584 582 . . 564 568 + BccI CCATC 572 573 . . 574 577 + TspEI AATT 573 577 . . 579 583 + MboII GAAGA 591 590 . . 587 590 + MseI TTAA 587 589 . . 598 602 + TspDTI ATGAA 613 611 . . 610 619 + XmnI GAANNNNTTC 614 614 . . 612 615 + TspEI AATT 611 615 . . 622 627 + AsuII TTCGAA 623 625 . . 623 626 + TaqI TCGA 623 625 . . 627 637 + FalI AAGNNNNNCTT 618 613 650 645 627 637 - FalI AAGNNNNNCTT 650 645 618 613 639 643 + DdeI CTNAG 639 642 . . 651 656 - Eco57MI CTGRAG 636 634 . . 651 656 - GsuI CTGGAG 636 634 . . 653 657 - BsrI ACTGG 653 651 . . 674 678 + TspDTI ATGAA 689 687 . . 681 686 + Hpy178III* TCNNGA 682 684 . . 681 686 + BspHI TCATGA 681 685 . . 682 685 + FatI CATG 681 685 . . 682 685 + NlaIII CATG 685 681 . . 682 685 + CviAII CATG 682 684 . . 691 695 + Hpy188I TCNGA 693 692 . . 712 715 + MaeI CTAG 712 714 . . 720 729 + XmnI GAANNNNTTC 724 724 . . 725 729 + TfiI GAWTC 725 728 . . 725 729 + HinfI GANTC 725 728 . . 727 732 + AsuII TTCGAA 728 730 . . 728 731 + TaqI TCGA 728 730 . . 735 740 + EcoRV GATATC 737 737 . . 748 752 + TspDTI ATGAA 763 761 . . 749 760 + BdaI TGANNNNNNTCA 738 736 772 770 750 759 + XmnI GAANNNNTTC 754 754 . . 749 760 - BdaI TGANNNNNNTCA 772 770 738 736 769 772 + CviRI* TGCA 770 770 . . 776 779 + TspEI AATT 775 779 . . 780 784 + MboII GAAGA 792 791 . . 780 785 - Ksp632I* CTCTTC 775 778 . . 780 786 - SapI GCTCTTC 775 778 . . 788 792 + TfiI GAWTC 788 791 . . 788 792 + HinfI GANTC 788 791 . . 785 788 - AciI CCGC 785 787 . . 783 788 - BsrBI CCGCTC 785 785 . . 791 796 + Hpy178III* TCNNGA 792 794 . . 799 802 + MseI TTAA 799 801 . . 801 806 + SspI AATATT 803 803 . . 821 824 + TaiI ACGT 824 820 . . 821 824 + SetI ASST 824 820 . . 821 824 + MaeII ACGT 821 823 . . 829 839 - Hin4I GAYNNNNNVTC 852 847 820 815 871 875 + TspDTI ATGAA 886 884 . . 874 879 + SspI AATATT 876 876 . . 887 890 + TspEI AATT 886 890 . . 912 915 + CviJI RGCY 913 913 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 243 690 # # AciI: # [CCGC] # 92 148 693 # # AcyI: # [GRCGYC] # 353 580 # # AflIII: # [ACRYGT] # 197 736 # # AluI: # [AGCT] # 92 322 519 # # ApoI: # [RAATTY] # 98 359 476 # # AsuI*: # [GGNCC] # 5 36 892 # # AsuII: # [TTCGAA] # 105 205 623 # # AvaII: # [GGWCC] # 41 892 # # BbvII*: # [GAAGAC] # 443 490 # # BccI: # [CCATC] # 27 135 197 213 361 # # Bce83I*: # [CTTGAG] # 280 653 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 54 161 650 # # BetI*: # [WCCGGW] # 49 884 # # BglI: # [GCCNNNNNGGC] # 44 889 # # BinI*: # [GGATC] # 392 541 # # BmgT120I: # [GGNCC] # 5 37 891 # # BsaXI: # [ACNNNNNCTCC] # 30 99 804 # # BseGI: # [GGATG] # 426 507 # # BseMII: # [CTCAG] # 430 503 # # BseRI: # [GAGGAG] # 424 509 # # BsgI: # [GTGCAG] # 271 662 # # BslFI: # [GGGAC] # 193 740 # # BspCNI: # [CTCAG] # 431 502 # # BspHI: # [TCATGA] # 252 256 425 # # BspMI: # [ACCTGC] # 243 690 # # BsrBI: # [CCGCTC] # 148 785 # # BsrI: # [ACTGG] # 280 653 # # BstKTI: # [GATC] # 74 313 546 # # BtrI: # [CACGTC] # 198 735 # # Cac8I: # [GCNNGC] # 392 541 # # Cfr10I: # [RCCGGY] # 38 895 # # Csp6I: # [GTAC] # 342 591 # # CviAII: # [CATG] # 95 161 251 426 # # CviJI: # [RGCY] # 9 20 38 92 275 499 # # CviRI*: # [TGCA] # 59 64 163 252 395 # # DdeI: # [CTNAG] # 200 294 439 # # DpnI: # [GATC] # 73 313 547 # # Eco57I: # [CTGAAG] # 308 625 # # Eco57MI: # [CTGRAG] # 297 308 328 # # EcoRV: # [GATATC] # 196 737 # # FalI: # [AAGNNNNNCTT] # 0 0 32 283 618 # # FatI: # [CATG] # 95 161 252 425 # # FokI: # [GGATG] # 419 514 # # GsuI: # [CTGGAG] # 297 636 # # HaeIII: # [GGCC] # 38 895 # # HgaI: # [GACGC] # 361 572 # # HgiAI*: # [GWGCWC] # 137 796 # # HgiCI*: # [GGYRCC] # 115 818 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 77 81 156 523 # # HindII: # [GTYRAC] # 284 649 # # HindIII: # [AAGCTT] # 320 613 # # HinfI: # [GANTC] # 63 115 145 165 445 # # HpaII: # [CCGG] # 5 6 39 883 # # HphI: # [GGTGA] # 210 723 # # Hpy166II: # [GTNNAC] # 284 649 # # Hpy178III*: # [TCNNGA] # 77 106 110 141 243 256 # # Hpy188I: # [TCNGA] # 20 32 217 240 424 # # Hpy99I: # [CGWCG] # 355 578 # # Ksp632I*: # [CTCTTC] # 158 775 # # MaeI: # [CTAG] # 104 221 608 # # MaeII: # [ACGT] # 69 112 182 197 373 # # MaeIII: # [GTNAC] # 82 851 # # MboI: # [GATC] # 71 313 549 # # MboII: # [GAAGA] # 30 82 141 148 201 331 # # MlyI: # [GAGTC] # 130 159 644 # # MmeI: # [TCCRAC] # 207 349 377 # # MnlI: # [CCTC] # 59 343 531 # # MseI: # [TTAA] # 75 134 212 253 259 # # MwoI: # [GCNNNNNNNGC] # 44 889 # # NlaIII: # [CATG] # 95 161 248 429 # # NlaIV: # [GGNNCC] # 48 69 816 # # PleI: # [GAGTC] # 129 159 645 # # PpiI: # [GAACNNNNNCTC] # 32 400 501 # # RsaI: # [GTAC] # 343 590 # # RsrII: # [CGGWCCG] # 41 892 # # SapI: # [GCTCTTC] # 158 775 # # ScaI: # [AGTACT] # 343 590 # # SduI: # [GDGCHC] # 137 796 # # SetI: # [ASST] # 11 12 24 32 55 57 # 70 92 109 130 341 # # SmlI: # [CTYRAG] # 295 638 # # SspI: # [AATATT] # 57 73 311 492 # # TaiI: # [ACGT] # 69 109 182 200 373 # # TaqI: # [TCGA] # 70 105 130 205 423 # # TaqII: # [CACCCA] # 103 830 # # TatI: # [WGTACW] # 341 592 # # TfiI: # [GAWTC] # 63 145 725 # # Tsp4CI*: # [ACNGT] # 184 749 # # TspDTI: # [ATGAA] # 47 74 76 123 168 445 # # TspEI: # [AATT] # 28 38 47 50 71 98 # 111 116 164 210 # # TstI: # [CACNNNNNNTCC] # 32 99 802 # # XhoII: # [RGATCY] # 384 549 # # XmnI: # [GAANNNNTTC] # 30 110 179 214 400 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 933 # Reported_sequences: 1 # Reported_hitcount: 190 #---------------------------------------