Restriction Fragments for CTK3/YML112W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

CTK3/YML112W on chromosome XIII from coordinates 45063 to 45953.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27027.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + HinfI GANTC 4 7 . . 9 14 + Hpy178III* TCNNGA 10 12 . . 15 18 + SetI ASST 18 14 . . 15 18 + CviJI RGCY 16 16 . . 15 18 + AluI AGCT 16 16 . . 17 20 + MaeI CTAG 17 19 . . 26 29 + TspEI AATT 25 29 . . 28 32 - TspDTI ATGAA 18 16 . . 34 42 + AlwNI CAGNNNCTG 39 36 . . 35 38 + SetI ASST 38 34 . . 35 41 + PpuMI RGGWCCY 36 39 . . 35 41 + DraII RGGNCCY 36 39 . . 36 40 + BmgT120I GGNCC 37 38 . . 36 40 + AvaII GGWCC 36 39 . . 36 40 + AsuI* GGNCC 36 39 . . 38 43 + Hpy178III* TCNNGA 39 41 . . 40 45 + Eco57MI CTGRAG 61 59 . . 40 45 + Eco57I CTGAAG 61 59 . . 42 46 + MboII GAAGA 54 53 . . 47 50 + SetI ASST 50 46 . . 47 52 + BspMI ACCTGC 56 60 . . 50 53 + CviRI* TGCA 51 51 . . 51 61 + MwoI GCNNNNNNNGC 57 54 . . 57 61 + HgaI GACGC 66 71 . . 60 64 + BlsI GCNGC 62 61 . . 60 64 + BisI GCNGC 61 62 . . 60 64 + TseI GCWGC 60 63 . . 62 65 + CviRI* TGCA 63 63 . . 60 64 - BbvI GCAGC 47 51 . . 61 66 - BsgI GTGCAG 46 44 . . 76 80 + HinfI GANTC 76 79 . . 74 78 - BsmAI GTCTC 68 72 . . 76 80 - PleI GAGTC 70 71 . . 76 80 - MlyI GAGTC 70 70 . . 74 84 - Hin4I GAYNNNNNVTC 97 92 65 60 92 96 + Hpy99I CGWCG 96 91 . . 93 98 + SalI GTCGAC 93 97 . . 93 98 + Hpy166II GTNNAC 95 95 . . 93 98 + HindII GTYRAC 95 95 . . 93 98 + AccI GTMKAC 94 96 . . 94 97 + TaqI TCGA 94 96 . . 108 112 + Tsp4CI* ACNGT 110 109 . . 117 127 + MwoI GCNNNNNNNGC 123 120 . . 113 118 - BtgZI GCGATG 98 102 . . 125 128 + SetI ASST 128 124 . . 125 128 + CviJI RGCY 126 126 . . 125 128 + AluI AGCT 126 126 . . 144 148 + Tsp4CI* ACNGT 146 145 . . 153 158 + SmlI CTYRAG 153 157 . . 153 158 + Bce83I* CTTGAG 174 172 . . 162 167 + SfeI* CTRYAG 162 166 . . 178 181 - MnlI CCTC 171 170 . . 200 203 + TaqI TCGA 200 202 . . 209 220 + BstXI CCANNNNNNTGG 216 212 . . 210 219 + MslI CAYNNNNRTG 214 214 . . 210 213 + FatI CATG 209 213 . . 210 213 + NlaIII CATG 213 209 . . 210 213 + CviAII CATG 210 212 . . 211 215 + TspDTI ATGAA 226 224 . . 213 217 + MboII GAAGA 225 224 . . 219 224 + NlaIV GGNNCC 221 221 . . 219 223 + BmgT120I GGNCC 220 221 . . 219 223 + AvaII GGWCC 219 222 . . 219 223 + AsuI* GGNCC 219 222 . . 216 220 - BccI CCATC 210 211 . . 226 229 + MaeI CTAG 226 228 . . 234 238 + BsrI ACTGG 239 237 . . 239 242 + TaiI ACGT 242 238 . . 239 242 + SetI ASST 242 238 . . 239 242 + MaeII ACGT 239 241 . . 280 283 + AciI CCGC 280 282 . . 285 290 + TsoI TARCCA 301 299 . . 286 289 + CviJI RGCY 287 287 . . 304 308 + MaeIII GTNAC 303 308 . . 316 319 + MseI TTAA 316 318 . . 333 336 + FatI CATG 332 336 . . 333 336 + NlaIII CATG 336 332 . . 333 336 + CviAII CATG 333 335 . . 356 359 + SetI ASST 359 355 . . 356 359 + CviJI RGCY 357 357 . . 356 359 + AluI AGCT 357 357 . . 357 367 + MwoI GCNNNNNNNGC 363 360 . . 372 376 + BssKI CCNGG 371 376 . . 372 376 + BseBI CCWGG 373 374 . . 372 376 + ScrFI CCNGG 373 374 . . 372 376 + EcoRII CCWGG 371 376 . . 376 386 + Hin4I GAYNNNNNVTC 367 362 399 394 377 381 + BsrI ACTGG 382 380 . . 376 386 - Hin4I GAYNNNNNVTC 399 394 367 362 387 390 + MnlI CCTC 397 396 . . 389 394 + Hpy178III* TCNNGA 390 392 . . 393 397 + TfiI GAWTC 393 396 . . 393 397 + HinfI GANTC 393 396 . . 399 402 + CviJI RGCY 400 400 . . 403 406 + SetI ASST 406 402 . . 413 416 + SetI ASST 416 412 . . 413 416 + CviJI RGCY 414 414 . . 413 416 + AluI AGCT 414 414 . . 447 451 + Hpy99I CGWCG 451 446 . . 448 452 + HgaI GACGC 457 462 . . 448 453 + AcyI GRCGYC 449 451 . . 460 464 + Tsp45I GTSAC 459 464 . . 460 464 + MaeIII GTNAC 459 464 . . 458 462 - HgaI GACGC 447 452 . . 472 476 + BccI CCATC 480 481 . . 475 479 + Hpy188I TCNGA 477 476 . . 493 497 + HgaI GACGC 502 507 . . 502 505 + SetI ASST 505 501 . . 502 508 + SexAI ACCWGGT 502 507 . . 503 507 + BssKI CCNGG 502 507 . . 503 507 + BseBI CCWGG 504 505 . . 503 507 + ScrFI CCNGG 504 505 . . 503 507 + EcoRII CCWGG 502 507 . . 507 510 + Csp6I GTAC 507 509 . . 507 510 + RsaI GTAC 508 508 . . 509 513 + Tsp4CI* ACNGT 511 510 . . 510 516 + TspRI CASTGNN 516 507 . . 515 520 + Hpy178III* TCNNGA 516 518 . . 525 528 - MnlI CCTC 518 517 . . 547 551 + TfiI GAWTC 547 550 . . 547 551 + HinfI GANTC 547 550 . . 554 557 + CviRI* TGCA 555 555 . . 551 556 - BsrDI GCAATG 550 548 . . 560 571 + PpiI GAACNNNNNCTC 552 547 584 579 562 566 + TspGWI ACGGA 577 575 . . 565 569 + PleI GAGTC 573 574 . . 565 569 + MlyI GAGTC 574 574 . . 565 569 + HinfI GANTC 565 568 . . 567 571 + BsmAI GTCTC 572 576 . . 574 579 + SmlI CTYRAG 574 578 . . 574 579 + AflII CTTAAG 574 578 . . 575 578 + MseI TTAA 575 577 . . 584 588 - BsmAI GTCTC 578 582 . . 591 595 + MboII GAAGA 603 602 . . 596 599 + TspEI AATT 595 599 . . 600 603 + CviJI RGCY 601 601 . . 610 615 + EcoP15I CAGCAG 640 642 . . 619 622 + TspEI AATT 618 622 . . 622 626 + Hpy188I TCNGA 624 623 . . 624 635 + BcgI CGANNNNNNTGC 613 611 647 645 636 639 + DpnI GATC 637 637 . . 636 639 + BstKTI GATC 638 636 . . 636 639 + MboI GATC 635 639 . . 642 646 + BssKI CCNGG 641 646 . . 642 646 + ScrFI CCNGG 643 644 . . 642 646 + CauII* CCSGG 643 644 . . 643 646 + HpaII CCGG 643 645 . . 654 658 + HgaI GACGC 663 668 . . 657 661 + BlsI GCNGC 659 658 . . 657 661 + BisI GCNGC 658 659 . . 657 661 + BbvI GCAGC 669 673 . . 657 661 + TseI GCWGC 657 660 . . 659 662 + CviJI RGCY 660 660 . . 669 672 + CviRI* TGCA 670 670 . . 683 686 + CviRI* TGCA 684 684 . . 689 693 + FokI GGATG 702 706 . . 689 693 + BseGI GGATG 695 693 . . 690 694 - BccI CCATC 684 685 . . 710 713 + CviRI* TGCA 711 711 . . 709 714 - BsgI GTGCAG 694 692 . . 718 721 + SetI ASST 721 717 . . 717 720 - MnlI CCTC 710 709 . . 721 724 + TaqI TCGA 721 723 . . 727 731 - BsmAI GTCTC 721 725 . . 738 741 + Csp6I GTAC 738 740 . . 738 741 + RsaI GTAC 739 739 . . 743 746 + TaqI TCGA 743 745 . . 748 754 + DraII RGGNCCY 749 752 . . 749 752 + CviJI RGCY 750 750 . . 749 752 + HaeIII GGCC 750 750 . . 749 753 + BmgT120I GGNCC 750 751 . . 749 753 + AsuI* GGNCC 749 752 . . 752 755 + MnlI CCTC 762 761 . . 754 759 + MmeI TCCRAC 779 777 . . 755 769 + XcmI CCANNNNNNNNNTGG 762 761 . . 765 769 + BsrI ACTGG 770 768 . . 768 772 + FokI GGATG 781 785 . . 768 772 + BseGI GGATG 774 772 . . 770 774 + TspDTI ATGAA 785 783 . . 772 776 + TfiI GAWTC 772 775 . . 772 776 + HinfI GANTC 772 775 . . 775 779 + Hpy188I TCNGA 777 776 . . 778 783 + ApoI RAATTY 778 782 . . 779 782 + TspEI AATT 778 782 . . 781 786 + AhaIII* TTTAAA 783 783 . . 782 785 + MseI TTAA 782 784 . . 786 791 + HindIII AAGCTT 786 790 . . 787 790 + SetI ASST 790 786 . . 787 790 + CviJI RGCY 788 788 . . 787 790 + AluI AGCT 788 788 . . 792 797 + Hpy166II GTNNAC 794 794 . . 795 799 + HgaI GACGC 804 809 . . 814 818 + TfiI GAWTC 814 817 . . 814 818 + HinfI GANTC 814 817 . . 817 822 + Hpy178III* TCNNGA 818 820 . . 817 822 + BetI* WCCGGW 817 821 . . 817 822 + BspMII* TCCGGA 817 821 . . 818 821 + HpaII CCGG 818 820 . . 852 855 + CviJI RGCY 853 853 . . 855 860 + AhaIII* TTTAAA 857 857 . . 856 859 + MseI TTAA 856 858 . . 864 869 - BsrDI GCAATG 863 861 . . 870 882 + BarI GAAGNNNNNNTAC 862 857 . . 874 878 + SfaNI GCATC 883 887 . . 870 874 - Hin4II* CCTTC 864 863 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 94 797 # # AciI: # [CCGC] # 280 611 # # AcyI: # [GRCGYC] # 442 449 # # AflII: # [CTTAAG] # 317 574 # # AhaIII*: # [TTTAAA] # 34 74 783 # # AluI: # [AGCT] # 16 57 103 110 231 374 # # AlwNI: # [CAGNNNCTG] # 39 852 # # ApoI: # [RAATTY] # 113 778 # # AsuI*: # [GGNCC] # 36 142 183 530 # # AvaII: # [GGWCC] # 36 183 672 # # BarI: # [GAAGNNNNNNTAC] # 29 862 # # BbvI: # [GCAGC] # 47 222 622 # # BccI: # [CCATC] # 204 207 210 270 # # Bce83I*: # [CTTGAG] # 174 717 # # BcgI: # [CGANNNNNNTGC] # 34 244 613 # # BetI*: # [WCCGGW] # 74 817 # # BisI: # [GCNGC] # 61 233 597 # # BlsI: # [GCNGC] # 62 232 597 # # BmgT120I: # [GGNCC] # 37 141 183 530 # # BseBI: # [CCWGG] # 131 373 387 # # BseGI: # [GGATG] # 79 117 695 # # BsgI: # [GTGCAG] # 46 197 648 # # BsmAI: # [GTCTC] # 6 68 143 170 504 # # BspMI: # [ACCTGC] # 56 835 # # BspMII*: # [TCCGGA] # 74 817 # # BsrDI: # [GCAATG] # 28 313 550 # # BsrI: # [ACTGG] # 121 143 239 388 # # BssKI: # [CCNGG] # 131 139 250 371 # # BstKTI: # [GATC] # 253 638 # # BstXI: # [CCANNNNNNTGG] # 216 675 # # BtgZI: # [GCGATG] # 98 793 # # CauII*: # [CCSGG] # 248 643 # # Csp6I: # [GTAC] # 153 231 507 # # CviAII: # [CATG] # 123 210 558 # # CviJI: # [RGCY] # 14 16 38 38 43 59 # 65 70 90 110 161 187 # # CviRI*: # [TGCA] # 12 14 27 51 115 180 # 492 # # DpnI: # [GATC] # 254 637 # # DraII: # [RGGNCCY] # 36 142 713 # # Eco57I: # [CTGAAG] # 61 830 # # Eco57MI: # [CTGRAG] # 61 830 # # EcoP15I: # [CAGCAG] # 251 640 # # EcoRII: # [CCWGG] # 131 371 389 # # FatI: # [CATG] # 123 209 559 # # FokI: # [GGATG] # 79 110 702 # # HaeIII: # [GGCC] # 141 750 # # HgaI: # [GACGC] # 10 45 66 87 141 161 # 381 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 65 270 # 492 # # Hin4II*: # [CCTTC] # 27 864 # # HindII: # [GTYRAC] # 95 796 # # HindIII: # [AAGCTT] # 105 786 # # HinfI: # [GANTC] # 4 18 42 72 77 154 # 207 317 # # HpaII: # [CCGG] # 73 175 643 # # Hpy166II: # [GTNNAC] # 95 97 699 # # Hpy178III*: # [TCNNGA] # 10 29 73 126 302 351 # # Hpy188I: # [TCNGA] # 114 147 153 477 # # Hpy99I: # [CGWCG] # 96 355 440 # # MaeI: # [CTAG] # 17 209 665 # # MaeII: # [ACGT] # 239 652 # # MaeIII: # [GTNAC] # 156 303 432 # # MboI: # [GATC] # 256 635 # # MboII: # [GAAGA] # 54 171 288 378 # # MlyI: # [GAGTC] # 70 317 504 # # MmeI: # [TCCRAC] # 112 779 # # MnlI: # [CCTC] # 52 121 129 171 192 226 # # MseI: # [TTAA] # 35 74 207 259 316 # # MslI: # [CAYNNNNRTG] # 214 677 # # MwoI: # [GCNNNNNNNGC] # 57 66 240 528 # # NlaIII: # [CATG] # 123 213 555 # # NlaIV: # [GGNNCC] # 221 670 # # PleI: # [GAGTC] # 70 318 503 # # PpiI: # [GAACNNNNNCTC] # 32 307 552 # # PpuMI: # [RGGWCCY] # 36 855 # # RsaI: # [GTAC] # 152 231 508 # # SalI: # [GTCGAC] # 93 798 # # ScrFI: # [CCNGG] # 131 139 248 373 # # SetI: # [ASST] # 10 12 18 20 47 69 # 78 89 101 114 117 216 # # SexAI: # [ACCWGGT] # 389 502 # # SfaNI: # [GCATC] # 8 883 # # SfeI*: # [CTRYAG] # 162 729 # # SmlI: # [CTYRAG] # 153 317 421 # # TaiI: # [ACGT] # 242 649 # # TaqI: # [TCGA] # 22 94 106 148 521 # # TfiI: # [GAWTC] # 42 77 154 225 393 # # TseI: # [GCWGC] # 60 234 597 # # TsoI: # [TARCCA] # 301 590 # # Tsp45I: # [GTSAC] # 432 459 # # Tsp4CI*: # [ACNGT] # 36 110 365 380 # # TspDTI: # [ATGAA] # 18 106 208 559 # # TspEI: # [AATT] # 23 25 113 160 570 # # TspGWI: # [ACGGA] # 314 577 # # TspRI: # [CASTGNN] # 375 516 # # XcmI: # [CCANNNNNNNNNTGG] # 129 762 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 891 # Reported_sequences: 1 # Reported_hitcount: 205 #---------------------------------------