Restriction Fragments for YML031C-A



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The currently selected gene/sequence is :

YML031C-A on chromosome XIII from coordinates 214421 to 214086.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.1990.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + BclI TGATCA 2 6 . . 3 6 + DpnI GATC 4 4 . . 3 6 + BstKTI GATC 5 3 . . 3 6 + MboI GATC 2 6 . . 17 20 + MaeI CTAG 17 19 . . 19 22 + SetI ASST 22 18 . . 22 25 + MseI TTAA 22 24 . . 27 30 + SetI ASST 30 26 . . 27 30 + CviJI RGCY 28 28 . . 27 30 + AluI AGCT 28 28 . . 33 38 + SspI AATATT 35 35 . . 56 60 - BsrI ACTGG 56 54 . . 64 73 + MslI CAYNNNNRTG 68 68 . . 68 72 + Tsp4CI* ACNGT 70 69 . . 70 74 + HphI GGTGA 82 81 . . 80 90 + MwoI GCNNNNNNNGC 86 83 . . 85 90 + Cac8I GCNNGC 87 87 . . 87 92 + EcoP15I CAGCAG 117 119 . . 89 94 + BtsI GCAGTG 96 94 . . 90 96 + TspRI CASTGNN 96 87 . . 101 111 + BsiYI* CCNNNNNNNGG 107 104 . . 101 111 + PflMI CCANNNNNTGG 107 104 . . 98 102 - HphI GGTGA 90 89 . . 103 107 + Tsp4CI* ACNGT 105 104 . . 121 126 + NdeI CATATG 122 124 . . 124 128 + TspDTI ATGAA 139 137 . . 134 137 + SetI ASST 137 133 . . 135 138 + MnlI CCTC 145 144 . . 139 143 + MaeIII GTNAC 138 143 . . 153 157 + TfiI GAWTC 153 156 . . 153 157 + HinfI GANTC 153 156 . . 164 167 + CviRI* TGCA 165 165 . . 165 175 + MwoI GCNNNNNNNGC 171 168 . . 167 172 + AflIII ACRYGT 167 171 . . 168 173 + BtrI CACGTC 170 170 . . 169 172 + TaiI ACGT 172 168 . . 169 172 + SetI ASST 172 168 . . 169 172 + MaeII ACGT 169 171 . . 170 174 + Hpy99I CGWCG 174 169 . . 180 184 - BccI CCATC 174 175 . . 189 192 + Csp6I GTAC 189 191 . . 189 192 + RsaI GTAC 190 190 . . 190 195 + BsaAI YACGTR 192 192 . . 191 194 + TaiI ACGT 194 190 . . 191 194 + SetI ASST 194 190 . . 191 194 + MaeII ACGT 191 193 . . 191 196 + AflIII ACRYGT 191 195 . . 193 198 + Hpy166II GTNNAC 195 195 . . 195 198 + Csp6I GTAC 195 197 . . 195 198 + RsaI GTAC 196 196 . . 197 201 + Tsp4CI* ACNGT 199 198 . . 198 203 + DsaI* CCRYGG 198 202 . . 198 203 + SecI* CCNNGG 198 202 . . 203 207 + TfiI GAWTC 203 206 . . 203 207 + HinfI GANTC 203 206 . . 216 221 + StyI CCWWGG 216 220 . . 216 221 + SecI* CCNNGG 216 220 . . 220 225 + AcyI GRCGYC 221 223 . . 221 225 - HgaI GACGC 210 215 . . 236 241 + Cac8I GCNNGC 238 238 . . 259 263 - MboII GAAGA 251 250 . . 258 263 - BbvII* GAAGAC 251 255 . . 279 284 - BtgZI GCGATG 264 268 . . 289 293 + PleI GAGTC 297 298 . . 289 293 + MlyI GAGTC 298 298 . . 289 293 + HinfI GANTC 289 292 . . 295 301 + SapI GCTCTTC 302 305 . . 296 301 + Ksp632I* CTCTTC 302 305 . . 297 301 - MboII GAAGA 289 288 . . 310 314 + TfiI GAWTC 310 313 . . 310 314 + HinfI GANTC 310 313 . . 313 318 + Hpy178III* TCNNGA 314 316 . . 315 318 + TaqI TCGA 315 317 . . #--------------------------------------- # # # AcyI: # [GRCGYC] # 115 221 # # AflIII: # [ACRYGT] # 24 145 167 # # AluI: # [AGCT] # 28 308 # # BbvII*: # [GAAGAC] # 85 251 # # BccI: # [CCATC] # 162 174 # # BclI: # [TGATCA] # 2 334 # # BsaAI: # [YACGTR] # 144 192 # # BsiYI*: # [CCNNNNNNNGG] # 107 229 # # BsrI: # [ACTGG] # 56 280 # # BstKTI: # [GATC] # 5 331 # # BtgZI: # [GCGATG] # 72 264 # # BtrI: # [CACGTC] # 166 170 # # BtsI: # [GCAGTG] # 96 240 # # Cac8I: # [GCNNGC] # 87 98 151 # # Csp6I: # [GTAC] # 6 141 189 # # CviJI: # [RGCY] # 28 308 # # CviRI*: # [TGCA] # 165 171 # # DpnI: # [GATC] # 4 332 # # DsaI*: # [CCRYGG] # 138 198 # # EcoP15I: # [CAGCAG] # 117 219 # # HgaI: # [GACGC] # 126 210 # # HinfI: # [GANTC] # 21 26 50 86 153 # # HphI: # [GGTGA] # 8 82 246 # # Hpy166II: # [GTNNAC] # 141 195 # # Hpy178III*: # [TCNNGA] # 22 314 # # Hpy99I: # [CGWCG] # 162 174 # # Ksp632I*: # [CTCTTC] # 34 302 # # MaeI: # [CTAG] # 17 319 # # MaeII: # [ACGT] # 22 145 169 # # MaeIII: # [GTNAC] # 138 198 # # MboI: # [GATC] # 2 334 # # MboII: # [GAAGA] # 38 47 251 # # MlyI: # [GAGTC] # 38 298 # # MnlI: # [CCTC] # 145 191 # # MseI: # [TTAA] # 22 314 # # MslI: # [CAYNNNNRTG] # 68 268 # # MwoI: # [GCNNNNNNNGC] # 85 86 165 # # NdeI: # [CATATG] # 122 214 # # PflMI: # [CCANNNNNTGG] # 107 229 # # PleI: # [GAGTC] # 39 297 # # RsaI: # [GTAC] # 6 140 190 # # SapI: # [GCTCTTC] # 34 302 # # SecI*: # [CCNNGG] # 18 120 198 # # SetI: # [ASST] # 8 22 22 35 107 142 # # SspI: # [AATATT] # 35 301 # # StyI: # [CCWWGG] # 120 216 # # TaiI: # [ACGT] # 22 142 172 # # TaqI: # [TCGA] # 21 315 # # TfiI: # [GAWTC] # 26 50 107 153 # # Tsp4CI*: # [ACNGT] # 35 70 94 137 # # TspDTI: # [ATGAA] # 139 197 # # TspRI: # [CASTGNN] # 96 240 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 336 # Reported_sequences: 1 # Reported_hitcount: 73 #---------------------------------------