Restriction Fragments for GIS4/YML006C



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The currently selected gene/sequence is :

GIS4/YML006C on chromosome XIII from coordinates 258416 to 256092.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.23705.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 63 66 + Csp6I GTAC 63 65 . . 63 66 + RsaI GTAC 64 64 . . 67 70 + MseI TTAA 67 69 . . 74 77 + SetI ASST 77 73 . . 75 78 + MnlI CCTC 85 84 . . 76 80 + DdeI CTNAG 76 79 . . 76 80 + BspCNI CTCAG 89 87 . . 76 80 + BseMII CTCAG 90 88 . . 77 81 + Hpy188I TCNGA 79 78 . . 94 98 + MboII GAAGA 106 105 . . 113 116 + TspEI AATT 112 116 . . 115 118 + MseI TTAA 115 117 . . 130 133 + MseI TTAA 130 132 . . 141 145 + Hpy188I TCNGA 143 142 . . 151 160 + MslI CAYNNNNRTG 155 155 . . 160 164 + HphI GGTGA 172 171 . . 169 174 + SspI AATATT 171 171 . . 176 181 + CfrI YGGCCR 176 180 . . 177 180 + CviJI RGCY 178 178 . . 177 180 + HaeIII GGCC 178 178 . . 179 183 + BssKI CCNGG 178 183 . . 179 183 + BseBI CCWGG 180 181 . . 179 183 + ScrFI CCNGG 180 181 . . 179 183 + EcoRII CCWGG 178 183 . . 182 185 + CviJI RGCY 183 183 . . 182 185 + HaeIII GGCC 183 183 . . 192 196 + MboII GAAGA 204 203 . . 209 213 + BccI CCATC 217 218 . . 210 214 - FokI GGATG 196 200 . . 210 214 - BseGI GGATG 209 207 . . 225 230 + Hpy178III* TCNNGA 226 228 . . 225 230 + BspHI TCATGA 225 229 . . 226 229 + FatI CATG 225 229 . . 226 229 + NlaIII CATG 229 225 . . 226 229 + CviAII CATG 226 228 . . 227 231 + TspDTI ATGAA 242 240 . . 224 228 - TspDTI ATGAA 214 212 . . 229 233 + MboII GAAGA 241 240 . . 232 237 + EcoRV GATATC 234 234 . . 255 258 + MnlI CCTC 265 264 . . 251 255 - MboII GAAGA 243 242 . . 269 273 - TspDTI ATGAA 259 257 . . 273 278 + Hpy178III* TCNNGA 274 276 . . 281 284 + TspEI AATT 280 284 . . 286 295 + XmnI GAANNNNTTC 290 290 . . 305 308 + TspEI AATT 304 308 . . 307 310 + MseI TTAA 307 309 . . 319 325 + TspRI CASTGNN 325 316 . . 319 324 - BtsI GCAGTG 318 316 . . 330 335 + Hpy178III* TCNNGA 331 333 . . 330 335 + BspHI TCATGA 330 334 . . 331 334 + FatI CATG 330 334 . . 331 334 + NlaIII CATG 334 330 . . 331 334 + CviAII CATG 331 333 . . 332 336 + TspDTI ATGAA 347 345 . . 344 347 + SetI ASST 347 343 . . 345 355 + BsiYI* CCNNNNNNNGG 351 348 . . 352 356 + TspGWI ACGGA 367 365 . . 359 362 + SetI ASST 362 358 . . 360 363 + MnlI CCTC 370 369 . . 367 370 + TspEI AATT 366 370 . . 382 385 + SetI ASST 385 381 . . 391 396 + ApoI RAATTY 391 395 . . 392 395 + TspEI AATT 391 395 . . 400 405 + TatI WGTACW 400 404 . . 401 404 + Csp6I GTAC 401 403 . . 401 404 + RsaI GTAC 402 402 . . 423 428 + ApoI RAATTY 423 427 . . 424 427 + TspEI AATT 423 427 . . 428 433 + StyI CCWWGG 428 432 . . 428 433 + SecI* CCNNGG 428 432 . . 432 435 + CviJI RGCY 433 433 . . 435 440 + AhaIII* TTTAAA 437 437 . . 436 439 + MseI TTAA 436 438 . . 453 458 + ApoI RAATTY 453 457 . . 454 457 + TspEI AATT 453 457 . . 467 472 + TaqII CACCCA 483 481 . . 478 482 - MboII GAAGA 470 469 . . 480 484 - TspDTI ATGAA 470 468 . . 488 493 + HindIII AAGCTT 488 492 . . 489 492 + SetI ASST 492 488 . . 489 492 + CviJI RGCY 490 490 . . 489 492 + AluI AGCT 490 490 . . 503 506 + SetI ASST 506 502 . . 503 506 + CviJI RGCY 504 504 . . 503 506 + AluI AGCT 504 504 . . 505 508 + MaeI CTAG 505 507 . . 508 512 + TfiI GAWTC 508 511 . . 508 512 + HinfI GANTC 508 511 . . 514 519 + AclI AACGTT 515 517 . . 515 518 + TaiI ACGT 518 514 . . 515 518 + SetI ASST 518 514 . . 515 518 + MaeII ACGT 515 517 . . 520 524 + Hpy188I TCNGA 522 521 . . 522 526 + FokI GGATG 535 539 . . 522 526 + BseGI GGATG 528 526 . . 525 530 + BclI TGATCA 525 529 . . 526 536 + Hin4I GAYNNNNNVTC 517 512 549 544 526 529 + DpnI GATC 527 527 . . 526 529 + BstKTI GATC 528 526 . . 526 529 + MboI GATC 525 529 . . 529 535 + TspRI CASTGNN 535 526 . . 522 533 - TstI CACNNNNNNTCC 546 541 514 509 526 536 - Hin4I GAYNNNNNVTC 549 544 517 512 561 564 + SetI ASST 564 560 . . 561 564 + CviJI RGCY 562 562 . . 561 564 + AluI AGCT 562 562 . . 585 588 - MnlI CCTC 578 577 . . 600 605 + Hpy178III* TCNNGA 601 603 . . 600 605 + BetI* WCCGGW 600 604 . . 600 605 + BspMII* TCCGGA 600 604 . . 601 604 + HpaII CCGG 601 603 . . 607 611 + Tsp4CI* ACNGT 609 608 . . 619 624 + AclI AACGTT 620 622 . . 620 623 + TaiI ACGT 623 619 . . 620 623 + SetI ASST 623 619 . . 620 623 + MaeII ACGT 620 622 . . 622 626 + MaeIII GTNAC 621 626 . . 626 630 - FokI GGATG 612 616 . . 626 630 - BseGI GGATG 625 623 . . 628 632 - TspGWI ACGGA 618 616 . . 654 665 + BdaI TGANNNNNNTCA 643 641 677 675 661 664 + MnlI CCTC 671 670 . . 662 667 + SmlI CTYRAG 662 666 . . 663 668 + Hpy178III* TCNNGA 664 666 . . 654 665 - BdaI TGANNNNNNTCA 677 675 643 641 662 667 - Bce83I* CTTGAG 647 645 . . 671 675 + TspDTI ATGAA 686 684 . . 686 690 + TspDTI ATGAA 701 699 . . 688 692 + MboII GAAGA 700 699 . . 693 697 + FokI GGATG 706 710 . . 688 693 - Ksp632I* CTCTTC 683 686 . . 693 697 + BseGI GGATG 699 697 . . 691 694 - MnlI CCTC 684 683 . . 694 698 - SfaNI GCATC 684 688 . . 707 712 + SfeI* CTRYAG 707 711 . . 717 722 + ApoI RAATTY 717 721 . . 718 721 + TspEI AATT 717 721 . . 730 735 + Hpy178III* TCNNGA 731 733 . . 730 735 + NruI TCGCGA 732 732 . . 731 734 + FnuDII* CGCG 732 732 . . 736 740 + Tsp4CI* ACNGT 738 737 . . 742 746 + TfiI GAWTC 742 745 . . 742 746 + HinfI GANTC 742 745 . . 749 752 + CviJI RGCY 750 750 . . 767 772 + ApoI RAATTY 767 771 . . 768 771 + TspEI AATT 767 771 . . 775 779 + BccI CCATC 783 784 . . 788 792 + TspDTI ATGAA 803 801 . . 790 794 + MboII GAAGA 802 801 . . 790 795 + BbvII* GAAGAC 797 801 . . 801 805 + Tsp4CI* ACNGT 803 802 . . 804 808 + Tsp45I GTSAC 803 808 . . 804 808 + MaeIII GTNAC 803 808 . . 811 815 + Hpy188I TCNGA 813 812 . . 814 824 - Hin4I GAYNNNNNVTC 837 832 805 800 838 843 + Cac8I GCNNGC 840 840 . . 844 848 + MboII GAAGA 856 855 . . 844 849 + BbvII* GAAGAC 851 855 . . 847 857 + Hin4I GAYNNNNNVTC 838 833 870 865 848 851 + TaiI ACGT 851 847 . . 848 851 + SetI ASST 851 847 . . 848 851 + MaeII ACGT 848 850 . . 847 857 - Hin4I GAYNNNNNVTC 870 865 838 833 854 858 - FokI GGATG 840 844 . . 854 858 - BseGI GGATG 853 851 . . 864 870 + PfoI TCCNGGA 864 869 . . 865 869 + BssKI CCNGG 864 869 . . 865 869 + ScrFI CCNGG 866 867 . . 865 869 + CauII* CCSGG 866 867 . . 866 869 + HpaII CCGG 866 868 . . 875 878 + SetI ASST 878 874 . . 877 881 + DdeI CTNAG 877 880 . . 877 881 - BspCNI CTCAG 869 867 . . 877 881 - BseMII CTCAG 868 866 . . 883 886 + MseI TTAA 883 885 . . 893 897 + BssKI CCNGG 892 897 . . 893 897 + BseBI CCWGG 894 895 . . 893 897 + ScrFI CCNGG 894 895 . . 893 897 + EcoRII CCWGG 892 897 . . 897 900 + Hin6I GCGC 897 899 . . 897 900 + GlaI GCGC 898 898 . . 897 900 + HhaI GCGC 899 897 . . 898 901 + FnuDII* CGCG 899 899 . . 903 907 + FokI GGATG 916 920 . . 903 907 + BseGI GGATG 909 907 . . 901 904 - MnlI CCTC 894 893 . . 905 909 + TspDTI ATGAA 920 918 . . 906 917 + BdaI TGANNNNNNTCA 895 893 929 927 915 920 + Hpy178III* TCNNGA 916 918 . . 906 917 - BdaI TGANNNNNNTCA 929 927 895 893 918 922 + FokI GGATG 931 935 . . 918 922 + BseGI GGATG 924 922 . . 920 924 + TspDTI ATGAA 935 933 . . 930 934 + BinI* GGATC 938 939 . . 928 931 - MnlI CCTC 921 920 . . 931 934 + DpnI GATC 932 932 . . 931 934 + BstKTI GATC 933 931 . . 931 934 + MboI GATC 930 934 . . 944 948 + PleI GAGTC 952 953 . . 944 948 + MlyI GAGTC 953 953 . . 944 948 + HinfI GANTC 944 947 . . 946 950 + BsmAI GTCTC 951 955 . . 952 955 + FatI CATG 951 955 . . 952 955 + NlaIII CATG 955 951 . . 952 955 + CviAII CATG 952 954 . . 955 958 + DpnI GATC 956 956 . . 955 958 + BstKTI GATC 957 955 . . 955 958 + MboI GATC 954 958 . . 958 961 + MaeI CTAG 958 960 . . 960 963 + SetI ASST 963 959 . . 961 965 + HphI GGTGA 973 972 . . 962 966 + Tsp45I GTSAC 961 966 . . 962 966 + MaeIII GTNAC 961 966 . . 965 975 + BaeI ACNNNNGTAYC 954 949 987 982 973 978 + ClaI ATCGAT 974 976 . . 974 977 + TaqI TCGA 974 976 . . 976 979 + DpnI GATC 977 977 . . 976 979 + BstKTI GATC 978 976 . . 976 979 + MboI GATC 975 979 . . 969 979 - Hin4I GAYNNNNNVTC 992 987 960 955 993 996 + FatI CATG 992 996 . . 993 996 + NlaIII CATG 996 992 . . 993 996 + CviAII CATG 993 995 . . 1003 1008 - TaqII GACCGA 993 991 . . 1009 1012 + SetI ASST 1012 1008 . . 1009 1012 + CviJI RGCY 1010 1010 . . 1009 1012 + AluI AGCT 1010 1010 . . 1010 1020 + MwoI GCNNNNNNNGC 1016 1013 . . 1016 1019 + MaeI CTAG 1016 1018 . . 1018 1021 + SetI ASST 1021 1017 . . 1018 1021 + CviJI RGCY 1019 1019 . . 1018 1021 + AluI AGCT 1019 1019 . . 1020 1025 + SfeI* CTRYAG 1020 1024 . . 1046 1051 + SduI GDGCHC 1050 1046 . . 1046 1051 + BseSI GKGCMC 1050 1046 . . 1053 1058 + SfeI* CTRYAG 1053 1057 . . 1055 1059 + Tsp4CI* ACNGT 1057 1056 . . 1060 1063 + MseI TTAA 1060 1062 . . 1064 1074 + MwoI GCNNNNNNNGC 1070 1067 . . 1070 1074 + BlsI GCNGC 1072 1071 . . 1070 1074 + BisI GCNGC 1071 1072 . . 1070 1074 + BbvI GCAGC 1082 1086 . . 1070 1074 + TseI GCWGC 1070 1073 . . 1072 1075 + CviJI RGCY 1073 1073 . . 1073 1078 + NmeAIII GCCGAG 1099 1097 . . 1074 1079 + SecI* CCNNGG 1074 1078 . . 1077 1080 + SetI ASST 1080 1076 . . 1076 1079 - MnlI CCTC 1069 1068 . . 1089 1092 + TaiI ACGT 1092 1088 . . 1089 1092 + SetI ASST 1092 1088 . . 1089 1092 + MaeII ACGT 1089 1091 . . 1099 1104 + ClaI ATCGAT 1100 1102 . . 1100 1103 + TaqI TCGA 1100 1102 . . 1102 1105 + DpnI GATC 1103 1103 . . 1102 1105 + BstKTI GATC 1104 1102 . . 1102 1105 + MboI GATC 1101 1105 . . 1105 1114 + MslI CAYNNNNRTG 1109 1109 . . 1110 1115 + BsmI GAATGC 1116 1114 . . 1117 1120 + SetI ASST 1120 1116 . . 1123 1127 + BsmAI GTCTC 1128 1132 . . 1126 1130 + Hpy188I TCNGA 1128 1127 . . 1130 1133 + TspEI AATT 1129 1133 . . 1139 1151 - PsrI GAACNNNNNNTAC 1163 1158 1131 1126 1151 1156 + SfeI* CTRYAG 1151 1155 . . 1159 1162 + TspEI AATT 1158 1162 . . 1162 1165 + MseI TTAA 1162 1164 . . 1171 1174 + TaqI TCGA 1171 1173 . . 1179 1189 + BsiYI* CCNNNNNNNGG 1185 1182 . . 1183 1188 + BseRI GAGGAG 1198 1196 . . 1183 1186 - MnlI CCTC 1176 1175 . . 1186 1191 + BseRI GAGGAG 1201 1199 . . 1186 1189 - MnlI CCTC 1179 1178 . . 1189 1192 - MnlI CCTC 1182 1181 . . 1195 1198 + TspEI AATT 1194 1198 . . 1198 1201 + MseI TTAA 1198 1200 . . 1200 1203 + TspEI AATT 1199 1203 . . 1202 1206 - TspDTI ATGAA 1192 1190 . . 1207 1216 + MslI CAYNNNNRTG 1211 1211 . . 1216 1220 + HgaI GACGC 1225 1230 . . 1216 1221 + AcyI GRCGYC 1217 1219 . . 1221 1232 + BstXI CCANNNNNNTGG 1228 1224 . . 1221 1226 + StyI CCWWGG 1221 1225 . . 1221 1226 + SecI* CCNNGG 1221 1225 . . 1231 1235 + BinI* GGATC 1239 1240 . . 1232 1235 + DpnI GATC 1233 1233 . . 1232 1235 + BstKTI GATC 1234 1232 . . 1232 1235 + MboI GATC 1231 1235 . . 1234 1239 + Hpy178III* TCNNGA 1235 1237 . . 1240 1243 - MnlI CCTC 1233 1232 . . 1246 1250 - BccI CCATC 1240 1241 . . 1252 1256 + MboII GAAGA 1264 1263 . . 1256 1259 + SetI ASST 1259 1255 . . 1252 1257 - Ksp632I* CTCTTC 1247 1250 . . 1255 1258 - MnlI CCTC 1248 1247 . . 1249 1259 - BsaXI ACNNNNNCTCC 1271 1268 1241 1238 1267 1276 + BsaBI GATNNNNATC 1271 1271 . . 1268 1272 + TspDTI ATGAA 1283 1281 . . 1270 1274 + MboII GAAGA 1282 1281 . . 1272 1277 + XhoII RGATCY 1272 1276 . . 1272 1277 + BglII AGATCT 1272 1276 . . 1273 1276 + DpnI GATC 1274 1274 . . 1273 1276 + BstKTI GATC 1275 1273 . . 1273 1276 + MboI GATC 1272 1276 . . 1279 1283 - MboII GAAGA 1271 1270 . . 1285 1289 + Hpy188I TCNGA 1287 1286 . . 1282 1286 - MboII GAAGA 1274 1273 . . 1293 1297 + DdeI CTNAG 1293 1296 . . 1293 1297 + BspCNI CTCAG 1306 1304 . . 1293 1297 + BseMII CTCAG 1307 1305 . . 1296 1301 + TatI WGTACW 1296 1300 . . 1296 1301 + ScaI AGTACT 1298 1298 . . 1297 1300 + Csp6I GTAC 1297 1299 . . 1297 1300 + RsaI GTAC 1298 1298 . . 1311 1315 + Tsp4CI* ACNGT 1313 1312 . . 1323 1326 + TspEI AATT 1322 1326 . . 1330 1334 + TfiI GAWTC 1330 1333 . . 1330 1334 + HinfI GANTC 1330 1333 . . 1336 1339 + MaeI CTAG 1336 1338 . . 1349 1352 + CviJI RGCY 1350 1350 . . 1350 1360 + MwoI GCNNNNNNNGC 1356 1353 . . 1351 1354 + MaeI CTAG 1351 1353 . . 1358 1361 + CviRI* TGCA 1359 1359 . . 1368 1373 + VspI ATTAAT 1369 1371 . . 1369 1372 + MseI TTAA 1369 1371 . . 1371 1374 + TspEI AATT 1370 1374 . . 1374 1379 + Hpy178III* TCNNGA 1375 1377 . . 1377 1382 + XhoII RGATCY 1377 1381 . . 1377 1382 + NlaIV GGNNCC 1379 1379 . . 1377 1382 + BamHI GGATCC 1377 1381 . . 1377 1381 + BinI* GGATC 1385 1386 . . 1378 1381 + DpnI GATC 1379 1379 . . 1378 1381 + BstKTI GATC 1380 1378 . . 1378 1381 + MboI GATC 1377 1381 . . 1380 1385 + Hpy178III* TCNNGA 1381 1383 . . 1378 1382 - BinI* GGATC 1372 1373 . . 1394 1399 + ApoI RAATTY 1394 1398 . . 1395 1398 + TspEI AATT 1394 1398 . . 1413 1417 + Tsp4CI* ACNGT 1415 1414 . . 1428 1431 + SetI ASST 1431 1427 . . 1438 1443 + Cac8I GCNNGC 1440 1440 . . 1444 1448 + MaeIII GTNAC 1443 1448 . . 1448 1458 + BsiYI* CCNNNNNNNGG 1454 1451 . . 1468 1472 + TfiI GAWTC 1468 1471 . . 1468 1472 + HinfI GANTC 1468 1471 . . 1474 1477 + TspEI AATT 1473 1477 . . 1483 1487 + TspDTI ATGAA 1498 1496 . . 1489 1492 + TspEI AATT 1488 1492 . . 1496 1501 + ApoI RAATTY 1496 1500 . . 1497 1500 + TspEI AATT 1496 1500 . . 1509 1514 + Hpy178III* TCNNGA 1510 1512 . . 1519 1522 + MaeI CTAG 1519 1521 . . 1522 1527 + EcoRV GATATC 1524 1524 . . 1526 1531 + Hpy178III* TCNNGA 1527 1529 . . 1532 1535 + DpnI GATC 1533 1533 . . 1532 1535 + BstKTI GATC 1534 1532 . . 1532 1535 + MboI GATC 1531 1535 . . 1544 1549 + ApoI RAATTY 1544 1548 . . 1545 1548 + TspEI AATT 1544 1548 . . 1562 1567 - TsoI TARCCA 1552 1550 . . 1570 1574 + Tsp45I GTSAC 1569 1574 . . 1570 1574 + MaeIII GTNAC 1569 1574 . . 1580 1583 + HpaII CCGG 1580 1582 . . 1589 1592 + SetI ASST 1592 1588 . . 1594 1598 + MboII GAAGA 1606 1605 . . 1597 1601 + MboII GAAGA 1609 1608 . . 1600 1605 + EcoRI GAATTC 1600 1604 . . 1600 1605 + ApoI RAATTY 1600 1604 . . 1601 1604 + TspEI AATT 1600 1604 . . 1615 1618 + TspEI AATT 1614 1618 . . 1630 1634 - Hin4II* CCTTC 1624 1623 . . 1650 1653 + SetI ASST 1653 1649 . . 1651 1654 + MnlI CCTC 1661 1660 . . 1653 1658 + XbaI TCTAGA 1653 1657 . . 1653 1658 + Hpy178III* TCNNGA 1654 1656 . . 1654 1657 + MaeI CTAG 1654 1656 . . 1657 1661 + TfiI GAWTC 1657 1660 . . 1657 1661 + HinfI GANTC 1657 1660 . . 1660 1664 + Hpy188I TCNGA 1662 1661 . . 1661 1665 + DdeI CTNAG 1661 1664 . . 1661 1665 - BspCNI CTCAG 1653 1651 . . 1661 1665 - BseMII CTCAG 1652 1650 . . 1672 1675 + MseI TTAA 1672 1674 . . 1683 1687 + FauI CCCGC 1691 1693 . . 1684 1687 + AciI CCGC 1684 1686 . . 1691 1696 + SspI AATATT 1693 1693 . . 1697 1702 + Hpy178III* TCNNGA 1698 1700 . . 1704 1707 + CviRI* TGCA 1705 1705 . . 1715 1719 + Tsp4CI* ACNGT 1717 1716 . . 1718 1722 + MaeIII GTNAC 1717 1722 . . 1739 1743 + TspDTI ATGAA 1754 1752 . . 1741 1745 + MboII GAAGA 1753 1752 . . 1741 1746 + BbvII* GAAGAC 1748 1752 . . 1748 1752 + TspDTI ATGAA 1763 1761 . . 1750 1754 + MboII GAAGA 1762 1761 . . 1750 1755 + BbvII* GAAGAC 1757 1761 . . 1759 1763 + HphI GGTGA 1771 1770 . . 1760 1764 + Tsp45I GTSAC 1759 1764 . . 1760 1764 + MaeIII GTNAC 1759 1764 . . 1756 1760 - BccI CCATC 1750 1751 . . 1765 1769 - SfaNI GCATC 1755 1759 . . 1777 1781 + Hpy188I TCNGA 1779 1778 . . 1782 1786 + Tsp4CI* ACNGT 1784 1783 . . 1789 1792 + CviJI RGCY 1790 1790 . . 1789 1792 + HaeIII GGCC 1790 1790 . . 1789 1793 + BmgT120I GGNCC 1790 1791 . . 1789 1793 + AsuI* GGNCC 1789 1792 . . 1799 1804 + TatI WGTACW 1799 1803 . . 1799 1804 + Bsp1407I TGTACA 1799 1803 . . 1800 1803 + Csp6I GTAC 1800 1802 . . 1800 1803 + RsaI GTAC 1801 1801 . . 1811 1814 + MaeI CTAG 1811 1813 . . 1819 1823 + TfiI GAWTC 1819 1822 . . 1819 1823 + HinfI GANTC 1819 1822 . . 1825 1830 + AclI AACGTT 1826 1828 . . 1826 1829 + TaiI ACGT 1829 1825 . . 1826 1829 + SetI ASST 1829 1825 . . 1826 1829 + MaeII ACGT 1826 1828 . . 1828 1832 + MaeIII GTNAC 1827 1832 . . 1832 1837 + SfeI* CTRYAG 1832 1836 . . 1855 1859 + Tsp4CI* ACNGT 1857 1856 . . 1858 1863 + Hpy166II GTNNAC 1860 1860 . . 1862 1866 + Tsp4CI* ACNGT 1864 1863 . . 1879 1884 - BsmI GAATGC 1879 1877 . . 1894 1897 + TspEI AATT 1893 1897 . . 1900 1904 + MaeIII GTNAC 1899 1904 . . 1909 1914 + ClaI ATCGAT 1910 1912 . . 1910 1913 + TaqI TCGA 1910 1912 . . 1925 1929 + MaeIII GTNAC 1924 1929 . . 1932 1937 - MmeI TCCRAC 1913 1911 . . 1950 1953 + SetI ASST 1953 1949 . . 1951 1955 + HphI GGTGA 1963 1962 . . 1957 1962 + TatI WGTACW 1957 1961 . . 1958 1961 + Csp6I GTAC 1958 1960 . . 1958 1961 + RsaI GTAC 1959 1959 . . 1960 1964 + Tsp4CI* ACNGT 1962 1961 . . 1973 1978 + Hpy166II GTNNAC 1975 1975 . . 1977 1980 + SetI ASST 1980 1976 . . 1978 1981 + MnlI CCTC 1988 1987 . . 1981 1985 - FokI GGATG 1967 1971 . . 1981 1985 - BseGI GGATG 1980 1978 . . 1989 2000 + DrdI GACNNNNNNGTC 1995 1993 . . 1995 1999 + Tsp4CI* ACNGT 1997 1996 . . 1999 2002 + TaqI TCGA 1999 2001 . . 2018 2021 + MnlI CCTC 2028 2027 . . 2020 2023 + TaqI TCGA 2020 2022 . . 2022 2027 + ApoI RAATTY 2022 2026 . . 2023 2026 + TspEI AATT 2022 2026 . . 2028 2033 + TsoI TARCCA 2044 2042 . . 2029 2032 + CviJI RGCY 2030 2030 . . 2040 2044 + MboII GAAGA 2052 2051 . . 2040 2045 + BbvII* GAAGAC 2047 2051 . . 2049 2054 + ApoI RAATTY 2049 2053 . . 2050 2053 + TspEI AATT 2049 2053 . . 2061 2064 + SetI ASST 2064 2060 . . 2065 2068 + TspEI AATT 2064 2068 . . 2069 2074 + StyI CCWWGG 2069 2073 . . 2069 2074 + SecI* CCNNGG 2069 2073 . . 2075 2079 + MaeIII GTNAC 2074 2079 . . 2081 2085 + TspDTI ATGAA 2096 2094 . . 2089 2092 + TspEI AATT 2088 2092 . . 2123 2127 + BccI CCATC 2131 2132 . . 2131 2141 + MwoI GCNNNNNNNGC 2137 2134 . . 2141 2146 + DsaI* CCRYGG 2141 2145 . . 2141 2146 + SecI* CCNNGG 2141 2145 . . 2141 2151 + BsiYI* CCNNNNNNNGG 2147 2144 . . 2140 2144 - BceAI ACGGC 2125 2127 . . 2149 2152 + SetI ASST 2152 2148 . . 2150 2154 + BmgT120I GGNCC 2151 2152 . . 2150 2154 + AvaII GGWCC 2150 2153 . . 2150 2154 + AsuI* GGNCC 2150 2153 . . 2157 2167 + FalI AAGNNNNNCTT 2148 2143 2180 2175 2157 2167 - FalI AAGNNNNNCTT 2180 2175 2148 2143 2169 2172 + SetI ASST 2172 2168 . . 2188 2191 + MseI TTAA 2188 2190 . . 2192 2196 + TauI GCSGC 2195 2192 . . 2192 2196 + BlsI GCNGC 2194 2193 . . 2192 2196 + BisI GCNGC 2193 2194 . . 2192 2195 - AciI CCGC 2192 2194 . . 2212 2215 + FatI CATG 2211 2215 . . 2212 2215 + NlaIII CATG 2215 2211 . . 2212 2215 + CviAII CATG 2212 2214 . . 2237 2242 - MmeI TCCRAC 2218 2216 . . 2242 2247 + ApoI RAATTY 2242 2246 . . 2243 2246 + TspEI AATT 2242 2246 . . 2246 2251 + Hpy178III* TCNNGA 2247 2249 . . 2251 2255 + TspDTI ATGAA 2266 2264 . . 2257 2260 + SetI ASST 2260 2256 . . 2261 2264 + MseI TTAA 2261 2263 . . 2265 2269 + TfiI GAWTC 2265 2268 . . 2265 2269 + HinfI GANTC 2265 2268 . . 2276 2280 + Tsp4CI* ACNGT 2278 2277 . . 2277 2283 + TspRI CASTGNN 2283 2274 . . 2285 2290 + PstI CTGCAG 2289 2285 . . 2285 2290 + SfeI* CTRYAG 2285 2289 . . 2286 2289 + CviRI* TGCA 2287 2287 . . 2289 2292 + SetI ASST 2292 2288 . . 2291 2294 + Csp6I GTAC 2291 2293 . . 2291 2294 + RsaI GTAC 2292 2292 . . 2287 2292 - BspMI ACCTGC 2278 2282 . . 2293 2297 + TspGWI ACGGA 2308 2306 . . 2303 2307 + DdeI CTNAG 2303 2306 . . 2303 2307 + BspCNI CTCAG 2316 2314 . . 2303 2307 + BseMII CTCAG 2317 2315 . . 2315 2319 + BccI CCATC 2323 2324 . . 2319 2322 + FatI CATG 2318 2322 . . 2319 2322 + NlaIII CATG 2322 2318 . . 2319 2322 + CviAII CATG 2319 2321 . . #--------------------------------------- # # # AciI: # [CCGC] # 133 508 1684 # # AclI: # [AACGTT] # 105 499 515 1206 # # AcyI: # [GRCGYC] # 1108 1217 # # AhaIII*: # [TTTAAA] # 437 1888 # # AluI: # [AGCT] # 9 14 58 448 490 1306 # # ApoI: # [RAATTY] # 27 30 32 48 50 56 # 83 102 193 264 391 422 # 627 # # AsuI*: # [GGNCC] # 175 361 1789 # # AvaII: # [GGWCC] # 175 2150 # # BaeI: # [ACNNNNGTAYC] # 33 954 1338 # # BamHI: # [GGATCC] # 948 1377 # # BbvI: # [GCAGC] # 1082 1243 # # BbvII*: # [GAAGAC] # 9 54 278 290 797 897 # # BccI: # [CCATC] # 2 192 217 381 457 510 # 566 # # Bce83I*: # [CTTGAG] # 647 1678 # # BceAI: # [ACGGC] # 200 2125 # # BclI: # [TGATCA] # 525 1800 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 218 643 1396 # # BetI*: # [WCCGGW] # 600 1725 # # BglII: # [AGATCT] # 1053 1272 # # BinI*: # [GGATC] # 13 133 301 938 940 # # BisI: # [GCNGC] # 132 1071 1122 # # BlsI: # [GCNGC] # 131 1072 1122 # # BmgT120I: # [GGNCC] # 174 361 1790 # # BsaBI: # [GATNNNNATC] # 1054 1271 # # BsaXI: # [ACNNNNNCTCC] # 30 1054 1241 # # BseBI: # [CCWGG] # 180 714 1431 # # BseGI: # [GGATG] # 15 56 74 97 154 209 # 319 345 1056 # # BseMII: # [CTCAG] # 8 90 345 439 665 778 # # BseRI: # [GAGGAG] # 3 1124 1198 # # BseSI: # [GKGCMC] # 1050 1275 # # BsiYI*: # [CCNNNNNNNGG] # 178 269 351 693 834 # # BsmAI: # [GTCTC] # 177 951 1197 # # BsmI: # [GAATGC] # 446 763 1116 # # Bsp1407I: # [TGTACA] # 526 1799 # # BspCNI: # [CTCAG] # 9 89 347 437 663 780 # # BspHI: # [TCATGA] # 105 225 1995 # # BspMI: # [ACCTGC] # 47 2278 # # BspMII*: # [TCCGGA] # 600 1725 # # BssKI: # [CCNGG] # 28 178 686 1433 # # BstKTI: # [GATC] # 21 24 41 105 126 130 # 154 405 528 791 # # BstXI: # [CCANNNNNNTGG] # 1097 1228 # # BtsI: # [GCAGTG] # 318 2007 # # Cac8I: # [GCNNGC] # 600 840 885 # # CauII*: # [CCSGG] # 866 1459 # # CfrI: # [YGGCCR] # 176 2149 # # ClaI: # [ATCGAT] # 126 415 810 974 # # Csp6I: # [GTAC] # 34 63 158 333 338 503 # 896 # # CviAII: # [CATG] # 6 41 105 107 226 621 # 1219 # # CviJI: # [RGCY] # 5 9 14 54 57 58 # 178 188 240 250 260 277 # 295 440 # # CviRI*: # [TGCA] # 3 38 346 582 1356 # # DdeI: # [CTNAG] # 22 76 368 416 642 801 # # DpnI: # [GATC] # 21 24 41 105 126 130 # 154 405 527 792 # # DrdI: # [GACNNNNNNGTC] # 330 1995 # # DsaI*: # [CCRYGG] # 184 2141 # # EcoRI: # [GAATTC] # 725 1600 # # EcoRII: # [CCWGG] # 178 714 1433 # # EcoRV: # [GATATC] # 234 801 1290 # # FalI: # [AAGNNNNNCTT] # 0 0 32 145 2148 # # FatI: # [CATG] # 7 41 105 107 225 621 # 1219 # # FauI: # [CCCGC] # 634 1691 # # FnuDII*: # [CGCG] # 167 732 1426 # # FokI: # [GGATG] # 15 76 77 94 134 196 # 339 358 1036 # # GlaI: # [GCGC] # 898 1427 # # HaeIII: # [GGCC] # 5 178 535 1607 # # HgaI: # [GACGC] # 1100 1225 # # HhaI: # [GCGC] # 899 1426 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 1 32 # 32 32 32 90 256 517 # 1333 # # Hin4II*: # [CCTTC] # 701 1624 # # Hin6I: # [GCGC] # 897 1428 # # HindIII: # [AAGCTT] # 488 1837 # # HinfI: # [GANTC] # 60 138 162 189 202 234 # 386 446 508 # # HpaII: # [CCGG] # 265 601 714 745 # # HphI: # [GGTGA] # 172 192 362 798 801 # # Hpy166II: # [GTNNAC] # 115 350 1860 # # Hpy178III*: # [TCNNGA] # 6 17 44 48 57 63 # 67 78 127 129 140 185 # 226 270 319 549 # # Hpy188I: # [TCNGA] # 64 79 117 159 291 315 # 375 379 546 # # Ksp632I*: # [CTCTTC] # 564 683 1078 # # MaeI: # [CTAG] # 15 58 135 157 168 320 # 453 505 514 # # MaeII: # [ACGT] # 105 228 241 499 515 737 # # MaeIII: # [GTNAC] # 25 42 68 72 126 148 # 150 158 182 251 482 621 # # MboI: # [GATC] # 21 24 41 105 126 130 # 154 405 525 794 # # MboII: # [GAAGA] # 2 3 3 7 8 9 # 37 54 98 102 106 144 # 227 230 273 290 324 408 # # MlyI: # [GAGTC] # 953 1372 # # MmeI: # [TCCRAC] # 107 305 1913 # # MnlI: # [CCTC] # 3 3 13 15 27 40 # 51 85 93 105 107 148 # 180 208 210 297 327 413 # # MseI: # [TTAA] # 15 36 48 64 67 73 # 102 129 171 177 177 303 # 447 516 # # MslI: # [CAYNNNNRTG] # 102 155 954 1114 # # MwoI: # [GCNNNNNNNGC] # 54 188 286 781 1016 # # NlaIII: # [CATG] # 3 41 105 107 229 621 # 1219 # # NlaIV: # [GGNNCC] # 946 1379 # # NmeAIII: # [GCCGAG] # 1099 1226 # # NruI: # [TCGCGA] # 732 1593 # # PfoI: # [TCCNGGA] # 864 1461 # # PleI: # [GAGTC] # 952 1373 # # PsrI: # [GAACNNNNNNTAC] # 32 1131 1162 # # PstI: # [CTGCAG] # 36 2289 # # RsaI: # [GTAC] # 33 64 158 333 338 503 # 896 # # ScaI: # [AGTACT] # 1027 1298 # # ScrFI: # [CCNGG] # 28 180 686 1431 # # SduI: # [GDGCHC] # 1050 1275 # # SecI*: # [CCNNGG] # 72 147 184 428 646 848 # # SetI: # [ASST] # 9 12 12 14 15 20 # 23 27 27 28 32 33 # 46 49 59 59 61 77 # 84 85 88 88 107 124 # 139 161 172 176 228 270 # # SfaNI: # [GCATC] # 570 684 1071 # # SfeI*: # [CTRYAG] # 33 40 98 313 453 681 # 707 # # SmlI: # [CTYRAG] # 662 1663 # # SspI: # [AATATT] # 171 632 1522 # # StyI: # [CCWWGG] # 256 428 793 848 # # TaiI: # [ACGT] # 105 228 241 496 518 737 # # TaqI: # [TCGA] # 21 71 89 126 305 739 # 974 # # TaqII: # [CACCCA] # 483 510 1332 # # TatI: # [WGTACW] # 158 368 400 503 896 # # TauI: # [GCSGC] # 130 2195 # # TfiI: # [GAWTC] # 60 138 162 189 234 446 # 508 588 # # TseI: # [GCWGC] # 1070 1255 # # TsoI: # [TARCCA] # 281 492 1552 # # Tsp45I: # [GTSAC] # 158 190 566 608 803 # # Tsp4CI*: # [ACNGT] # 7 35 47 65 67 73 # 98 102 129 254 256 281 # 302 609 # # TspDTI: # [ATGAA] # 9 15 15 17 28 59 # 88 91 102 117 123 170 # 214 215 216 256 257 333 # # TspEI: # [AATT] # 5 8 14 15 15 24 # 24 24 25 27 29 30 # 32 36 48 48 50 56 # 62 79 83 112 123 129 # 154 168 264 279 362 # # TspGWI: # [ACGGA] # 17 251 367 1690 # # TspRI: # [CASTGNN] # 42 210 325 1748 # # TstI: # [CACNNNNNNTCC] # 32 514 1779 # # VspI: # [ATTAAT] # 956 1369 # # XbaI: # [TCTAGA] # 672 1653 # # XhoII: # [RGATCY] # 105 948 1272 # # XmnI: # [GAANNNNTTC] # 290 2035 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2325 # Reported_sequences: 1 # Reported_hitcount: 509 #---------------------------------------