Restriction Fragments for YLRCtau1



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The currently selected gene/sequence is :

YLRCtau1 on chromosome XII from coordinates 599844 to 599570.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21646.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 16 + BclI TGATCA 11 15 . . 12 15 + DpnI GATC 13 13 . . 12 15 + BstKTI GATC 14 12 . . 12 15 + MboI GATC 11 15 . . 16 21 + VspI ATTAAT 17 19 . . 17 20 + MseI TTAA 17 19 . . 27 30 + FatI CATG 26 30 . . 27 30 + NlaIII CATG 30 26 . . 27 30 + CviAII CATG 27 29 . . 41 45 + MaeIII GTNAC 40 45 . . 46 49 + TspEI AATT 45 49 . . 47 54 + SwaI ATTTAAAT 50 50 . . 48 53 + AhaIII* TTTAAA 50 50 . . 49 52 + MseI TTAA 49 51 . . 55 60 + Hpy166II GTNNAC 57 57 . . 72 75 + SetI ASST 75 71 . . 71 74 - MnlI CCTC 64 63 . . 74 79 + Hpy166II GTNNAC 76 76 . . 74 77 + Csp6I GTAC 74 76 . . 74 77 + RsaI GTAC 75 75 . . 79 83 + BssKI CCNGG 78 83 . . 79 83 + ScrFI CCNGG 80 81 . . 79 83 + CauII* CCSGG 80 81 . . 80 83 + HpaII CCGG 80 82 . . 83 88 + Hpy166II GTNNAC 85 85 . . 83 88 + BssNAI GTATAC 85 85 . . 83 88 + AccI GTMKAC 84 86 . . 87 90 + TaiI ACGT 90 86 . . 87 90 + SetI ASST 90 86 . . 87 90 + MaeII ACGT 87 89 . . 102 105 + CviRI* TGCA 103 103 . . 124 129 + SmlI CTYRAG 124 128 . . 124 129 + AflII CTTAAG 124 128 . . 125 128 + MseI TTAA 125 127 . . 129 134 + Cac8I GCNNGC 131 131 . . 135 138 + TspEI AATT 134 138 . . 138 142 + Hpy188I TCNGA 140 139 . . 147 153 + EspI* GCTNAGC 148 151 . . 148 152 + DdeI CTNAG 148 151 . . 151 154 + SetI ASST 154 150 . . 151 154 + CviJI RGCY 152 152 . . 151 154 + AluI AGCT 152 152 . . 148 152 - BspCNI CTCAG 140 138 . . 148 152 - BseMII CTCAG 139 137 . . 153 157 + DdeI CTNAG 153 156 . . 153 157 - BspCNI CTCAG 145 143 . . 153 157 - BseMII CTCAG 144 142 . . 158 161 + MseI TTAA 158 160 . . 189 192 + SetI ASST 192 188 . . 189 192 + CviJI RGCY 190 190 . . 189 192 + AluI AGCT 190 190 . . 205 208 - MnlI CCTC 198 197 . . 215 221 + BstEII GGTNACC 215 220 . . 216 220 + Tsp45I GTSAC 215 220 . . 216 220 + MaeIII GTNAC 215 220 . . 217 221 - HphI GGTGA 209 208 . . 236 240 + MaeIII GTNAC 235 240 . . 250 259 + MslI CAYNNNNRTG 254 254 . . 260 263 + MseI TTAA 260 262 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 84 191 # # AflII: # [CTTAAG] # 124 151 # # AhaIII*: # [TTTAAA] # 50 225 # # AluI: # [AGCT] # 38 85 152 # # BclI: # [TGATCA] # 11 264 # # BseMII: # [CTCAG] # 5 131 139 # # BspCNI: # [CTCAG] # 5 130 140 # # BssKI: # [CCNGG] # 78 197 # # BssNAI: # [GTATAC] # 85 190 # # BstEII: # [GGTNACC] # 60 215 # # BstKTI: # [GATC] # 14 261 # # Cac8I: # [GCNNGC] # 131 144 # # CauII*: # [CCSGG] # 80 195 # # Csp6I: # [GTAC] # 74 201 # # CviAII: # [CATG] # 27 248 # # CviJI: # [RGCY] # 38 85 152 # # CviRI*: # [TGCA] # 103 172 # # DdeI: # [CTNAG] # 5 122 148 # # DpnI: # [GATC] # 13 262 # # EspI*: # [GCTNAGC] # 127 148 # # FatI: # [CATG] # 26 249 # # HpaII: # [CCGG] # 80 195 # # HphI: # [GGTGA] # 66 209 # # Hpy166II: # [GTNNAC] # 9 19 57 190 # # Hpy188I: # [TCNGA] # 135 140 # # MaeII: # [ACGT] # 87 188 # # MaeIII: # [GTNAC] # 20 40 40 175 # # MboI: # [GATC] # 11 264 # # MnlI: # [CCTC] # 64 77 134 # # MseI: # [TTAA] # 15 17 32 33 76 102 # # MslI: # [CAYNNNNRTG] # 21 254 # # NlaIII: # [CATG] # 30 245 # # RsaI: # [GTAC] # 75 200 # # ScrFI: # [CCNGG] # 80 195 # # SetI: # [ASST] # 15 38 64 75 83 # # SmlI: # [CTYRAG] # 124 151 # # SwaI: # [ATTTAAAT] # 50 225 # # TaiI: # [ACGT] # 90 185 # # Tsp45I: # [GTSAC] # 60 215 # # TspEI: # [AATT] # 45 89 141 # # VspI: # [ATTAAT] # 17 258 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 275 # Reported_sequences: 1 # Reported_hitcount: 59 #---------------------------------------