Restriction Fragments for YLRCdelta9



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YLRCdelta9 on chromosome XII from coordinates 593035 to 592706.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.16674.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 28 31 + MseI TTAA 28 30 . . 31 36 + SpeI ACTAGT 31 35 . . 32 35 + MaeI CTAG 32 34 . . 46 51 + MluI ACGCGT 46 50 . . 46 51 + AflIII ACRYGT 46 50 . . 47 50 + FnuDII* CGCG 48 48 . . 87 90 + SetI ASST 90 86 . . 95 99 + MboII GAAGA 107 106 . . 100 103 + CviRI* TGCA 101 101 . . 98 102 - SfaNI GCATC 88 92 . . 111 114 + MseI TTAA 111 113 . . 119 122 + SetI ASST 122 118 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + AluI AGCT 120 120 . . 129 133 - Hin4II* CCTTC 123 122 . . 154 158 + TspDTI ATGAA 169 167 . . 168 172 + TspGWI ACGGA 183 181 . . 171 175 - Hin4II* CCTTC 165 164 . . 175 185 - Hin4I GAYNNNNNVTC 198 193 166 161 188 193 + SspI AATATT 190 190 . . 207 212 + ClaI ATCGAT 208 210 . . 208 211 + TaqI TCGA 208 210 . . 210 214 + TfiI GAWTC 210 213 . . 210 214 + HinfI GANTC 210 213 . . 234 241 + PspXI VCTCGAGB 235 239 . . 234 237 + MnlI CCTC 244 243 . . 234 241 + AbsI CCTCGAGG 235 239 . . 235 240 + SmlI CTYRAG 235 239 . . 235 240 + XhoI CTCGAG 235 239 . . 235 240 + BmeT110I CYCGRG 236 238 . . 235 240 + AvaI CYCGRG 235 239 . . 236 239 + TaqI TCGA 236 238 . . 238 243 + BseRI GAGGAG 253 251 . . 238 241 - MnlI CCTC 231 230 . . 249 252 + MaeI CTAG 249 251 . . 268 273 + SspI AATATT 270 270 . . 276 279 + CviJI RGCY 277 277 . . 280 283 + MseI TTAA 280 282 . . 293 297 + TfiI GAWTC 293 296 . . 293 297 + HinfI GANTC 293 296 . . 303 306 + TspEI AATT 302 306 . . 322 325 + TspEI AATT 321 325 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 95 235 # # AflIII: # [ACRYGT] # 46 284 # # AluI: # [AGCT] # 120 210 # # AvaI: # [CYCGRG] # 95 235 # # BmeT110I: # [CYCGRG] # 94 236 # # BseRI: # [GAGGAG] # 77 253 # # ClaI: # [ATCGAT] # 122 208 # # CviJI: # [RGCY] # 53 120 157 # # CviRI*: # [TGCA] # 101 229 # # FnuDII*: # [CGCG] # 48 282 # # Hin4I: # [GAYNNNNNVTC] # 32 132 166 # # Hin4II*: # [CCTTC] # 42 123 165 # # HinfI: # [GANTC] # 37 83 210 # # MaeI: # [CTAG] # 32 81 217 # # MboII: # [GAAGA] # 107 223 # # MluI: # [ACGCGT] # 46 284 # # MnlI: # [CCTC] # 13 86 231 # # MseI: # [TTAA] # 28 50 83 169 # # PspXI: # [VCTCGAGB] # 95 235 # # SetI: # [ASST] # 32 90 208 # # SfaNI: # [GCATC] # 88 242 # # SmlI: # [CTYRAG] # 95 235 # # SpeI: # [ACTAGT] # 31 299 # # SspI: # [AATATT] # 60 80 190 # # TaqI: # [TCGA] # 28 94 208 # # TfiI: # [GAWTC] # 37 83 210 # # TspDTI: # [ATGAA] # 161 169 # # TspEI: # [AATT] # 9 19 302 # # TspGWI: # [ACGGA] # 147 183 # # XhoI: # [CTCGAG] # 95 235 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 330 # Reported_sequences: 1 # Reported_hitcount: 42 #---------------------------------------