Restriction Fragments for YLRCdelta27



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The currently selected gene/sequence is :

YLRCdelta27 on chromosome XII from coordinates 1052632 to 1052303.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.18738.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 34 40 + TspRI CASTGNN 40 31 . . 33 37 - BsrI ACTGG 33 31 . . 41 44 + FatI CATG 40 44 . . 41 44 + NlaIII CATG 44 40 . . 41 44 + CviAII CATG 41 43 . . 39 43 - TspDTI ATGAA 29 27 . . 44 48 + MaeIII GTNAC 43 48 . . 60 65 + NdeI CATATG 61 63 . . 65 68 - AciI CCGC 65 67 . . 97 101 + Tsp4CI* ACNGT 99 98 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 104 108 + Hpy188I TCNGA 106 105 . . 110 113 + MseI TTAA 110 112 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 126 129 + CviRI* TGCA 127 127 . . 181 184 + TspEI AATT 180 184 . . 187 192 + SspI AATATT 189 189 . . 196 201 + BsgI GTGCAG 217 215 . . 197 200 + CviRI* TGCA 198 198 . . 209 213 + TfiI GAWTC 209 212 . . 209 213 + HinfI GANTC 209 212 . . 213 223 + BsiYI* CCNNNNNNNGG 219 216 . . 223 227 + TfiI GAWTC 223 226 . . 220 223 - MnlI CCTC 213 212 . . 223 227 + HinfI GANTC 223 226 . . 227 232 + NdeI CATATG 228 230 . . 225 229 - TspDTI ATGAA 215 213 . . 234 237 + MnlI CCTC 244 243 . . 235 240 + SmlI CTYRAG 235 239 . . 235 240 + XhoI CTCGAG 235 239 . . 235 240 + BmeT110I CYCGRG 236 238 . . 235 240 + AvaI CYCGRG 235 239 . . 236 239 + TaqI TCGA 236 238 . . 236 241 + Hpy178III* TCNNGA 237 239 . . 240 244 + MboII GAAGA 252 251 . . 240 249 + XmnI GAANNNNTTC 244 244 . . 246 251 - Eco57MI CTGRAG 231 229 . . 251 261 + MwoI GCNNNNNNNGC 257 254 . . 251 261 + BstAPI GCANNNNNTGC 257 254 . . 246 251 - Eco57I CTGAAG 231 229 . . 259 262 + CviRI* TGCA 260 260 . . 293 298 + ApoI RAATTY 293 297 . . 294 297 + TspEI AATT 293 297 . . 298 301 + MseI TTAA 298 300 . . 303 306 + TspEI AATT 302 306 . . 313 318 + ApoI RAATTY 313 317 . . 314 317 + TspEI AATT 313 317 . . 319 322 + SetI ASST 322 318 . . 321 324 + MaeI CTAG 321 323 . . 317 321 - HphI GGTGA 309 308 . . #--------------------------------------- # # # AciI: # [CCGC] # 65 265 # # AluI: # [AGCT] # 119 211 # # ApoI: # [RAATTY] # 17 20 293 # # AvaI: # [CYCGRG] # 95 235 # # BmeT110I: # [CYCGRG] # 94 236 # # BsgI: # [GTGCAG] # 113 217 # # BsiYI*: # [CCNNNNNNNGG] # 111 219 # # BsrI: # [ACTGG] # 33 297 # # BstAPI: # [GCANNNNNTGC] # 73 257 # # CviAII: # [CATG] # 41 289 # # CviJI: # [RGCY] # 119 211 # # CviRI*: # [TGCA] # 62 70 71 127 # # Eco57I: # [CTGAAG] # 99 231 # # Eco57MI: # [CTGRAG] # 99 231 # # FatI: # [CATG] # 40 290 # # Hin4I: # [GAYNNNNNVTC] # 32 83 215 # # HinfI: # [GANTC] # 14 107 209 # # HphI: # [GGTGA] # 21 309 # # Hpy178III*: # [TCNNGA] # 93 237 # # Hpy188I: # [TCNGA] # 106 224 # # MaeI: # [CTAG] # 9 321 # # MaeIII: # [GTNAC] # 43 287 # # MboII: # [GAAGA] # 78 252 # # MnlI: # [CCTC] # 31 86 213 # # MseI: # [TTAA] # 32 110 188 # # MwoI: # [GCNNNNNNNGC] # 73 257 # # NdeI: # [CATATG] # 61 102 167 # # NlaIII: # [CATG] # 44 286 # # SetI: # [ASST] # 8 121 201 # # SmlI: # [CTYRAG] # 95 235 # # SspI: # [AATATT] # 141 189 # # TaqI: # [TCGA] # 94 236 # # TfiI: # [GAWTC] # 14 107 209 # # Tsp4CI*: # [ACNGT] # 99 231 # # TspDTI: # [ATGAA] # 29 115 186 # # TspEI: # [AATT] # 9 11 17 113 180 # # TspRI: # [CASTGNN] # 40 290 # # XhoI: # [CTCGAG] # 95 235 # # XmnI: # [GAANNNNTTC] # 86 244 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 330 # Reported_sequences: 1 # Reported_hitcount: 52 #---------------------------------------