Restriction Fragments for YLR458W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YLR458W on chromosome XII from coordinates 1056455 to 1056835.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.9955.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 19 22 + CviRI* TGCA 20 20 . . 20 24 + SfaNI GCATC 29 33 . . 36 40 - TspDTI ATGAA 26 24 . . 60 64 + Hpy188I TCNGA 62 61 . . 68 73 + Hpy178III* TCNNGA 69 71 . . 82 87 + Hpy166II GTNNAC 84 84 . . 82 87 + HpaI GTTAAC 84 84 . . 82 87 + HindII GTYRAC 84 84 . . 83 86 + MseI TTAA 83 85 . . 86 89 + SetI ASST 89 85 . . 87 91 + Hin4II* CCTTC 97 96 . . 87 97 + BsiYI* CCNNNNNNNGG 93 90 . . 95 100 - TaqII CACCCA 85 83 . . 105 108 + MnlI CCTC 115 114 . . 115 118 + Csp6I GTAC 115 117 . . 115 118 + RsaI GTAC 116 116 . . 119 128 + XmnI GAANNNNTTC 123 123 . . 119 124 + ApoI RAATTY 119 123 . . 120 123 + TspEI AATT 119 123 . . 127 131 - MboII GAAGA 119 118 . . 128 133 - Eco57MI CTGRAG 113 111 . . 128 133 - Eco57I CTGAAG 113 111 . . 145 149 + Tsp45I GTSAC 144 149 . . 145 149 + MaeIII GTNAC 144 149 . . 148 151 + SetI ASST 151 147 . . 146 150 - HphI GGTGA 138 137 . . 154 157 + MaeI CTAG 154 156 . . 156 159 + SetI ASST 159 155 . . 185 189 + HgaI GACGC 194 199 . . 188 192 + SfaNI GCATC 197 201 . . 198 202 - MboII GAAGA 190 189 . . 208 213 + SmlI CTYRAG 208 212 . . 208 213 + AflII CTTAAG 208 212 . . 209 212 + MseI TTAA 209 211 . . 217 220 + DpnI GATC 218 218 . . 217 220 + BstKTI GATC 219 217 . . 217 220 + MboI GATC 216 220 . . 231 235 - TspDTI ATGAA 221 219 . . 239 245 + SanDI GGGWCCC 240 243 . . 239 245 + PpuMI RGGWCCY 240 243 . . 239 245 + DraII RGGNCCY 240 243 . . 239 243 + BslFI GGGAC 253 257 . . 239 244 + NlaIV GGNNCC 241 241 . . 240 245 + NlaIV GGNNCC 242 242 . . 240 244 + BmgT120I GGNCC 241 242 . . 240 244 + AvaII GGWCC 240 243 . . 240 244 + AsuI* GGNCC 240 243 . . 262 266 + BccI CCATC 270 271 . . 293 297 + Tsp4CI* ACNGT 295 294 . . 294 302 + AlwNI CAGNNNCTG 299 296 . . 296 300 - BslFI GGGAC 281 285 . . 302 305 + Csp6I GTAC 302 304 . . 302 305 + RsaI GTAC 303 303 . . 304 308 + TspGWI ACGGA 319 317 . . 337 340 + Csp6I GTAC 337 339 . . 337 340 + RsaI GTAC 338 338 . . 339 342 + SetI ASST 342 338 . . 345 350 + Ksp632I* CTCTTC 351 354 . . 349 353 + Hpy188I TCNGA 351 350 . . 346 350 - MboII GAAGA 338 337 . . 347 352 - Eco57MI CTGRAG 332 330 . . 347 352 - Eco57I CTGAAG 332 330 . . 367 370 + FatI CATG 366 370 . . 367 370 + NlaIII CATG 370 366 . . 367 370 + CviAII CATG 367 369 . . 365 369 - TspDTI ATGAA 355 353 . . #--------------------------------------- # # # AflII: # [CTTAAG] # 173 208 # # AlwNI: # [CAGNNNCTG] # 82 299 # # ApoI: # [RAATTY] # 119 262 # # AsuI*: # [GGNCC] # 141 240 # # AvaII: # [GGWCC] # 141 240 # # BccI: # [CCATC] # 111 270 # # BmgT120I: # [GGNCC] # 140 241 # # BsiYI*: # [CCNNNNNNNGG] # 93 288 # # BslFI: # [GGGAC] # 28 100 253 # # BstKTI: # [GATC] # 162 219 # # Csp6I: # [GTAC] # 35 44 115 187 # # CviAII: # [CATG] # 14 367 # # CviRI*: # [TGCA] # 20 361 # # DpnI: # [GATC] # 163 218 # # DraII: # [RGGNCCY] # 141 240 # # Eco57I: # [CTGAAG] # 49 113 219 # # Eco57MI: # [CTGRAG] # 49 113 219 # # FatI: # [CATG] # 15 366 # # HgaI: # [GACGC] # 187 194 # # Hin4II*: # [CCTTC] # 97 284 # # HindII: # [GTYRAC] # 84 297 # # HpaI: # [GTTAAC] # 84 297 # # HphI: # [GGTGA] # 138 243 # # Hpy166II: # [GTNNAC] # 84 297 # # Hpy178III*: # [TCNNGA] # 69 312 # # Hpy188I: # [TCNGA] # 30 62 289 # # Ksp632I*: # [CTCTTC] # 30 351 # # MaeI: # [CTAG] # 154 227 # # MaeIII: # [GTNAC] # 144 237 # # MboI: # [GATC] # 165 216 # # MboII: # [GAAGA] # 43 71 119 148 # # MnlI: # [CCTC] # 115 266 # # MseI: # [TTAA] # 83 126 172 # # NlaIII: # [CATG] # 11 370 # # NlaIV: # [GGNNCC] # 1 139 241 # # PpuMI: # [RGGWCCY] # 141 240 # # RsaI: # [GTAC] # 35 43 116 187 # # SanDI: # [GGGWCCC] # 141 240 # # SetI: # [ASST] # 8 39 62 89 183 # # SfaNI: # [GCATC] # 29 168 184 # # SmlI: # [CTYRAG] # 173 208 # # TaqII: # [CACCCA] # 85 296 # # Tsp45I: # [GTSAC] # 144 237 # # Tsp4CI*: # [ACNGT] # 86 295 # # TspDTI: # [ATGAA] # 26 26 134 195 # # TspEI: # [AATT] # 119 262 # # TspGWI: # [ACGGA] # 62 319 # # XmnI: # [GAANNNNTTC] # 123 258 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 381 # Reported_sequences: 1 # Reported_hitcount: 66 #---------------------------------------