Restriction Fragments for SIR3/YLR442C



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The currently selected gene/sequence is :

SIR3/YLR442C on chromosome XII from coordinates 1022251 to 1019315.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.19469.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 26 30 + Tsp4CI* ACNGT 28 27 . . 51 56 + BclI TGATCA 51 55 . . 52 55 + DpnI GATC 53 53 . . 52 55 + BstKTI GATC 54 52 . . 52 55 + MboI GATC 51 55 . . 60 63 - MnlI CCTC 53 52 . . 83 87 + MboII GAAGA 95 94 . . 86 90 + MboII GAAGA 98 97 . . 89 92 + DpnI GATC 90 90 . . 89 92 + BstKTI GATC 91 89 . . 89 92 + MboI GATC 88 92 . . 91 96 + Hpy178III* TCNNGA 92 94 . . 101 106 + BseRI GAGGAG 116 114 . . 101 104 - MnlI CCTC 94 93 . . 104 109 + BseRI GAGGAG 119 117 . . 104 107 - MnlI CCTC 97 96 . . 113 116 + TaiI ACGT 116 112 . . 113 116 + SetI ASST 116 112 . . 113 116 + MaeII ACGT 113 115 . . 106 116 - BsaXI ACNNNNNCTCC 128 125 98 95 120 124 + Hpy188I TCNGA 122 121 . . 128 133 + ApoI RAATTY 128 132 . . 129 132 + TspEI AATT 128 132 . . 133 137 + Hpy188I TCNGA 135 134 . . 136 140 - BccI CCATC 130 131 . . 153 157 - Hin4II* CCTTC 147 146 . . 157 161 + HphI GGTGA 169 168 . . 173 176 + MseI TTAA 173 175 . . 184 188 + Tsp45I GTSAC 183 188 . . 184 188 + MaeIII GTNAC 183 188 . . 187 191 + TspGWI ACGGA 202 200 . . 202 207 + Hpy166II GTNNAC 204 204 . . 202 207 + AccI GTMKAC 203 205 . . 210 213 + DpnI GATC 211 211 . . 210 213 + BstKTI GATC 212 210 . . 210 213 + MboI GATC 209 213 . . 214 217 + FatI CATG 213 217 . . 214 217 + NlaIII CATG 217 213 . . 214 217 + CviAII CATG 214 216 . . 210 214 - BinI* GGATC 204 205 . . 219 222 + DpnI GATC 220 220 . . 219 222 + BstKTI GATC 221 219 . . 219 222 + MboI GATC 218 222 . . 221 225 + Hpy188I TCNGA 223 222 . . 224 228 + HinfI GANTC 224 227 . . 224 228 - PleI GAGTC 218 219 . . 224 228 - MlyI GAGTC 218 218 . . 236 239 + MseI TTAA 236 238 . . 270 275 + SmlI CTYRAG 270 274 . . 270 275 + Bce83I* CTTGAG 291 289 . . 275 279 - BccI CCATC 269 270 . . 297 301 + Tsp4CI* ACNGT 299 298 . . 299 304 + TatI WGTACW 299 303 . . 300 303 + Csp6I GTAC 300 302 . . 300 303 + RsaI GTAC 301 301 . . 311 314 + TspEI AATT 310 314 . . 317 320 + CviJI RGCY 318 318 . . 317 320 + HaeIII GGCC 318 318 . . 321 326 + XhoII RGATCY 321 325 . . 321 326 + BglII AGATCT 321 325 . . 322 325 + DpnI GATC 323 323 . . 322 325 + BstKTI GATC 324 322 . . 322 325 + MboI GATC 321 325 . . 334 338 + MboII GAAGA 346 345 . . 337 340 + DpnI GATC 338 338 . . 337 340 + BstKTI GATC 339 337 . . 337 340 + MboI GATC 336 340 . . 340 344 - FokI GGATG 326 330 . . 340 344 - BseGI GGATG 339 337 . . 349 354 + ApoI RAATTY 349 353 . . 350 353 + TspEI AATT 349 353 . . 364 369 + ApoI RAATTY 364 368 . . 365 368 + TspEI AATT 364 368 . . 400 403 + MseI TTAA 400 402 . . 406 412 + EspI* GCTNAGC 407 410 . . 407 411 + DdeI CTNAG 407 410 . . 404 409 - EcoP15I CAGCAG 376 378 . . 410 413 + SetI ASST 413 409 . . 410 413 + CviJI RGCY 411 411 . . 410 413 + AluI AGCT 411 411 . . 407 411 - BspCNI CTCAG 399 397 . . 407 411 - BseMII CTCAG 398 396 . . 415 419 + Hpy188I TCNGA 417 416 . . 425 428 + CviJI RGCY 426 426 . . 427 432 + SmlI CTYRAG 427 431 . . 427 432 + AflII CTTAAG 427 431 . . 428 431 + MseI TTAA 428 430 . . 436 440 - TspDTI ATGAA 426 424 . . 441 444 + CviRI* TGCA 442 442 . . 438 443 - BsrDI GCAATG 437 435 . . 445 450 - MmeI TCCRAC 426 424 . . 451 459 + AlwNI CAGNNNCTG 456 453 . . 460 474 + XcmI CCANNNNNNNNNTGG 467 466 . . 463 467 + PleI GAGTC 471 472 . . 463 467 + MlyI GAGTC 472 472 . . 463 467 + HinfI GANTC 463 466 . . 465 469 + Tsp45I GTSAC 464 469 . . 465 469 + MaeIII GTNAC 464 469 . . 487 492 + ClaI ATCGAT 488 490 . . 488 491 + TaqI TCGA 488 490 . . 499 502 - MnlI CCTC 492 491 . . 514 517 + MaeI CTAG 514 516 . . 517 520 + Csp6I GTAC 517 519 . . 517 520 + RsaI GTAC 518 518 . . 519 522 + TaiI ACGT 522 518 . . 519 522 + SetI ASST 522 518 . . 519 522 + MaeII ACGT 519 521 . . 524 529 + EcoT22I ATGCAT 528 524 . . 525 528 + CviRI* TGCA 526 526 . . 526 531 + NspI RCATGY 530 526 . . 526 531 + Cac8I GCNNGC 528 528 . . 526 531 + SphI GCATGC 530 526 . . 527 530 + FatI CATG 526 530 . . 527 530 + NlaIII CATG 530 526 . . 527 530 + CviAII CATG 527 529 . . 531 542 + BcgI CGANNNNNNTGC 520 518 554 552 534 537 + SetI ASST 537 533 . . 541 544 - AciI CCGC 541 543 . . 553 556 + Csp6I GTAC 553 555 . . 553 556 + RsaI GTAC 554 554 . . 557 562 + MfeI CAATTG 557 561 . . 558 561 + TspEI AATT 557 561 . . 561 572 + BdaI TGANNNNNNTCA 550 548 584 582 561 572 - BdaI TGANNNNNNTCA 584 582 550 548 581 585 + MboII GAAGA 593 592 . . 581 586 - Ksp632I* CTCTTC 576 579 . . 597 602 + NlaIV GGNNCC 599 599 . . 610 614 + Hpy188I TCNGA 612 611 . . 614 618 + TspDTI ATGAA 629 627 . . 637 640 + Csp6I GTAC 637 639 . . 637 640 + RsaI GTAC 638 638 . . 640 650 + BsiYI* CCNNNNNNNGG 646 643 . . 640 650 + PflMI CCANNNNNTGG 646 643 . . 641 647 + TspRI CASTGNN 647 638 . . 640 644 - BsrI ACTGG 640 638 . . 654 658 + MboII GAAGA 666 665 . . 654 659 + BbvII* GAAGAC 661 665 . . 668 671 + SetI ASST 671 667 . . 669 673 + HphI GGTGA 681 680 . . 673 678 + EcoT22I ATGCAT 677 673 . . 674 677 + CviRI* TGCA 675 675 . . 672 676 - SfaNI GCATC 662 666 . . 681 685 - BccI CCATC 675 676 . . 694 699 + XhoII RGATCY 694 698 . . 694 699 + BglII AGATCT 694 698 . . 695 698 + DpnI GATC 696 696 . . 695 698 + BstKTI GATC 697 695 . . 695 698 + MboI GATC 694 698 . . 697 701 - MboII GAAGA 689 688 . . 709 712 + MaeI CTAG 709 711 . . 720 723 + SetI ASST 723 719 . . 721 725 + Hin4II* CCTTC 731 730 . . 727 731 + TspDTI ATGAA 742 740 . . 735 738 + TspEI AATT 734 738 . . 737 740 + MseI TTAA 737 739 . . 743 746 + TaqI TCGA 743 745 . . 744 755 + BcgI CGANNNNNNTGC 733 731 767 765 745 750 + SduI GDGCHC 749 745 . . 745 750 + HgiJII* GRGCYC 749 745 . . 746 749 + CviJI RGCY 747 747 . . 749 752 + MnlI CCTC 759 758 . . 752 757 + PstI CTGCAG 756 752 . . 752 757 + SfeI* CTRYAG 752 756 . . 753 756 + CviRI* TGCA 754 754 . . 764 767 + TaiI ACGT 767 763 . . 764 767 + SetI ASST 767 763 . . 764 767 + MaeII ACGT 764 766 . . 783 787 + Tsp4CI* ACNGT 785 784 . . 785 790 + Esp3I CGTCTC 791 795 . . 786 790 + BsmAI GTCTC 791 795 . . 788 792 + DdeI CTNAG 788 791 . . 788 792 + BspCNI CTCAG 801 799 . . 788 792 + BseMII CTCAG 802 800 . . 794 797 - MnlI CCTC 787 786 . . 799 802 + TaiI ACGT 802 798 . . 799 802 + SetI ASST 802 798 . . 799 802 + MaeII ACGT 799 801 . . 801 806 + Hpy166II GTNNAC 803 803 . . 801 806 + HindII GTYRAC 803 803 . . 811 814 + FatI CATG 810 814 . . 811 814 + NlaIII CATG 814 810 . . 811 814 + CviAII CATG 811 813 . . 819 822 + TspEI AATT 818 822 . . 832 836 + MboII GAAGA 844 843 . . 835 839 + PleI GAGTC 843 844 . . 835 839 + MlyI GAGTC 844 844 . . 835 839 + HinfI GANTC 835 838 . . 832 837 - Ksp632I* CTCTTC 827 830 . . 861 866 + AcyI GRCGYC 862 864 . . 862 866 - HgaI GACGC 851 856 . . 868 872 + Hin4II* CCTTC 878 877 . . 864 868 - BslFI GGGAC 849 853 . . 874 877 + Hin6I GCGC 874 876 . . 874 877 + GlaI GCGC 875 875 . . 874 877 + HhaI GCGC 876 874 . . 885 888 + SetI ASST 888 884 . . 887 892 + SfeI* CTRYAG 887 891 . . 883 887 - HphI GGTGA 875 874 . . 892 896 + TfiI GAWTC 892 895 . . 892 896 + HinfI GANTC 892 895 . . 897 902 + AsuII TTCGAA 898 900 . . 898 901 + TaqI TCGA 898 900 . . 895 899 - MboII GAAGA 887 886 . . 902 907 + ApoI RAATTY 902 906 . . 903 906 + TspEI AATT 902 906 . . 916 921 + XhoII RGATCY 916 920 . . 916 921 + BglII AGATCT 916 920 . . 917 920 + DpnI GATC 918 918 . . 917 920 + BstKTI GATC 919 917 . . 917 926 + BsaBI GATNNNNATC 921 921 . . 917 920 + MboI GATC 916 920 . . 936 939 + TspEI AATT 935 939 . . 943 947 + Hpy188I TCNGA 945 944 . . 946 949 - MnlI CCTC 939 938 . . 963 968 + Ksp632I* CTCTTC 969 972 . . 967 971 + Hpy188I TCNGA 969 968 . . 964 968 - MboII GAAGA 956 955 . . 955 968 - AjuI GAANNNNNNNTTGG 980 975 948 943 965 970 - Eco57MI CTGRAG 950 948 . . 965 970 - Eco57I CTGAAG 950 948 . . 976 980 + TfiI GAWTC 976 979 . . 976 980 + HinfI GANTC 976 979 . . 979 983 + Hpy188I TCNGA 981 980 . . 989 992 + CviJI RGCY 990 990 . . 998 1002 + TspDTI ATGAA 1013 1011 . . 1019 1024 + Hpy166II GTNNAC 1021 1021 . . 1023 1026 + SetI ASST 1026 1022 . . 1024 1028 + BssKI CCNGG 1023 1028 . . 1024 1028 + BseBI CCWGG 1025 1026 . . 1024 1029 + SecI* CCNNGG 1024 1028 . . 1024 1028 + ScrFI CCNGG 1025 1026 . . 1024 1028 + EcoRII CCWGG 1023 1028 . . 1034 1037 + CviJI RGCY 1035 1035 . . 1041 1045 + MboII GAAGA 1053 1052 . . 1041 1046 + BbvII* GAAGAC 1048 1052 . . 1039 1049 - Hin4I GAYNNNNNVTC 1062 1057 1030 1025 1049 1054 + SfeI* CTRYAG 1049 1053 . . 1060 1065 + Hpy166II GTNNAC 1062 1062 . . 1060 1065 + HindII GTYRAC 1062 1062 . . 1064 1068 + BceAI ACGGC 1080 1082 . . 1065 1070 + McrI* CGRYCG 1068 1066 . . 1065 1070 + XmaIII* CGGCCG 1065 1069 . . 1065 1070 + CfrI YGGCCR 1065 1069 . . 1066 1069 + CviJI RGCY 1067 1067 . . 1066 1069 + HaeIII GGCC 1067 1067 . . 1067 1072 + NmeAIII GCCGAG 1093 1091 . . 1074 1079 + ApoI RAATTY 1074 1078 . . 1075 1078 + TspEI AATT 1074 1078 . . 1086 1089 + CviRI* TGCA 1087 1087 . . 1095 1100 + Hpy178III* TCNNGA 1096 1098 . . 1111 1115 - BccI CCATC 1105 1106 . . 1118 1121 + CviJI RGCY 1119 1119 . . 1123 1127 + MboII GAAGA 1135 1134 . . 1123 1128 - Ksp632I* CTCTTC 1118 1121 . . 1126 1129 - MnlI CCTC 1119 1118 . . 1136 1140 + TspDTI ATGAA 1151 1149 . . 1147 1151 + Hpy188I TCNGA 1149 1148 . . 1156 1160 + Hpy188I TCNGA 1158 1157 . . 1160 1164 + TspDTI ATGAA 1175 1173 . . 1162 1174 + BarI GAAGNNNNNNTAC 1154 1149 1186 1181 1174 1178 - BsrI ACTGG 1174 1172 . . 1188 1191 + TaiI ACGT 1191 1187 . . 1188 1191 + SetI ASST 1191 1187 . . 1188 1191 + MaeII ACGT 1188 1190 . . 1192 1197 + HaeII RGCGCY 1196 1192 . . 1187 1192 - BtrI CACGTC 1189 1189 . . 1193 1196 + Hin6I GCGC 1193 1195 . . 1193 1196 + GlaI GCGC 1194 1194 . . 1193 1196 + HhaI GCGC 1195 1193 . . 1199 1203 + TspGWI ACGGA 1214 1212 . . 1205 1215 + MwoI GCNNNNNNNGC 1211 1208 . . 1208 1211 + TspEI AATT 1207 1211 . . 1221 1224 + TspEI AATT 1220 1224 . . 1239 1244 + Hpy178III* TCNNGA 1240 1242 . . 1252 1257 + ApoI RAATTY 1252 1256 . . 1253 1256 + TspEI AATT 1252 1256 . . 1264 1267 + TspEI AATT 1263 1267 . . 1271 1276 + SfeI* CTRYAG 1271 1275 . . 1291 1295 + TspDTI ATGAA 1306 1304 . . 1295 1299 + TspGWI ACGGA 1310 1308 . . 1308 1311 + SetI ASST 1311 1307 . . 1309 1313 + BssKI CCNGG 1308 1313 . . 1309 1313 + BseBI CCWGG 1310 1311 . . 1309 1313 + ScrFI CCNGG 1310 1311 . . 1309 1313 + EcoRII CCWGG 1308 1313 . . 1313 1318 + EcoRI GAATTC 1313 1317 . . 1313 1318 + ApoI RAATTY 1313 1317 . . 1314 1317 + TspEI AATT 1313 1317 . . 1318 1324 + PasI CCCWGGG 1319 1322 . . 1318 1323 + SecI* CCNNGG 1318 1322 . . 1319 1323 + BssKI CCNGG 1318 1323 . . 1319 1323 + BseBI CCWGG 1320 1321 . . 1319 1324 + SecI* CCNNGG 1319 1323 . . 1319 1323 + ScrFI CCNGG 1320 1321 . . 1319 1323 + EcoRII CCWGG 1318 1323 . . 1334 1339 + ApoI RAATTY 1334 1338 . . 1335 1338 + TspEI AATT 1334 1338 . . 1337 1341 - TspDTI ATGAA 1327 1325 . . 1345 1349 + TspDTI ATGAA 1360 1358 . . 1363 1373 + BsiYI* CCNNNNNNNGG 1369 1366 . . 1371 1374 + SetI ASST 1374 1370 . . 1377 1380 + CviRI* TGCA 1378 1378 . . 1425 1429 + Hpy188I TCNGA 1427 1426 . . 1431 1434 + TspEI AATT 1430 1434 . . 1439 1444 + Cfr10I RCCGGY 1439 1443 . . 1439 1444 + BetI* WCCGGW 1439 1443 . . 1439 1444 + AgeI ACCGGT 1439 1443 . . 1440 1443 + HpaII CCGG 1440 1442 . . 1446 1449 + CviRI* TGCA 1447 1447 . . 1462 1466 + MaeIII GTNAC 1461 1466 . . 1468 1472 + TfiI GAWTC 1468 1471 . . 1468 1472 + HinfI GANTC 1468 1471 . . 1472 1475 + MnlI CCTC 1482 1481 . . 1477 1482 + MfeI CAATTG 1477 1481 . . 1478 1481 + TspEI AATT 1477 1481 . . 1487 1490 + TspEI AATT 1486 1490 . . 1489 1492 + MseI TTAA 1489 1491 . . 1495 1505 + Hin4I GAYNNNNNVTC 1486 1481 1518 1513 1495 1505 + Eam1105I GACNNNNNGTC 1500 1499 . . 1500 1504 + Tsp4CI* ACNGT 1502 1501 . . 1495 1505 - Hin4I GAYNNNNNVTC 1518 1513 1486 1481 1506 1511 + HindIII AAGCTT 1506 1510 . . 1507 1510 + SetI ASST 1510 1506 . . 1507 1510 + CviJI RGCY 1508 1508 . . 1507 1510 + AluI AGCT 1508 1508 . . 1514 1517 + TaiI ACGT 1517 1513 . . 1514 1517 + SetI ASST 1517 1513 . . 1514 1517 + MaeII ACGT 1514 1516 . . 1518 1521 + CviRI* TGCA 1519 1519 . . 1519 1524 + Cac8I GCNNGC 1521 1521 . . 1521 1526 + StuI AGGCCT 1523 1523 . . 1522 1525 + CviJI RGCY 1523 1523 . . 1522 1525 + HaeIII GGCC 1523 1523 . . 1525 1530 + SmlI CTYRAG 1525 1529 . . 1525 1530 + Bce83I* CTTGAG 1546 1544 . . 1528 1531 - MnlI CCTC 1521 1520 . . 1546 1551 + NspI RCATGY 1550 1546 . . 1546 1551 + Cac8I GCNNGC 1548 1548 . . 1546 1551 + SphI GCATGC 1550 1546 . . 1547 1550 + FatI CATG 1546 1550 . . 1547 1550 + NlaIII CATG 1550 1546 . . 1547 1550 + CviAII CATG 1547 1549 . . 1550 1556 + SapI GCTCTTC 1557 1560 . . 1551 1556 + Ksp632I* CTCTTC 1557 1560 . . 1552 1556 - MboII GAAGA 1544 1543 . . 1554 1558 - TspDTI ATGAA 1544 1542 . . 1558 1563 + Hpy178III* TCNNGA 1559 1561 . . 1562 1566 + MboII GAAGA 1574 1573 . . 1567 1576 + XmnI GAANNNNTTC 1571 1571 . . 1581 1586 + ApoI RAATTY 1581 1585 . . 1582 1585 + TspEI AATT 1581 1585 . . 1586 1589 + MseI TTAA 1586 1588 . . 1599 1604 + ApoI RAATTY 1599 1603 . . 1600 1603 + TspEI AATT 1599 1603 . . 1609 1613 + TfiI GAWTC 1609 1612 . . 1606 1609 - MnlI CCTC 1599 1598 . . 1609 1613 + HinfI GANTC 1609 1612 . . 1614 1617 + TspEI AATT 1613 1617 . . 1621 1625 + TfiI GAWTC 1621 1624 . . 1621 1625 + HinfI GANTC 1621 1624 . . 1660 1663 - MnlI CCTC 1653 1652 . . 1674 1679 + SspI AATATT 1676 1676 . . 1680 1684 + Hin4II* CCTTC 1690 1689 . . 1683 1687 + Hpy188I TCNGA 1685 1684 . . 1694 1698 + Tsp4CI* ACNGT 1696 1695 . . 1705 1709 - MboII GAAGA 1697 1696 . . 1704 1709 - BbvII* GAAGAC 1697 1701 . . 1737 1740 + CviRI* TGCA 1738 1738 . . 1744 1748 + TfiI GAWTC 1744 1747 . . 1744 1748 + HinfI GANTC 1744 1747 . . 1753 1758 + ApoI RAATTY 1753 1757 . . 1754 1757 + TspEI AATT 1753 1757 . . 1760 1763 + SetI ASST 1763 1759 . . 1760 1763 + CviJI RGCY 1761 1761 . . 1760 1763 + AluI AGCT 1761 1761 . . 1779 1783 + FokI GGATG 1792 1796 . . 1779 1783 + BseGI GGATG 1785 1783 . . 1787 1792 + BclI TGATCA 1787 1791 . . 1788 1791 + DpnI GATC 1789 1789 . . 1788 1791 + BstKTI GATC 1790 1788 . . 1788 1791 + MboI GATC 1787 1791 . . 1792 1795 + SetI ASST 1795 1791 . . 1793 1796 + MnlI CCTC 1803 1802 . . 1794 1799 + Ksp632I* CTCTTC 1800 1803 . . 1790 1794 - HphI GGTGA 1782 1781 . . 1795 1799 - MboII GAAGA 1787 1786 . . 1800 1803 + CviJI RGCY 1801 1801 . . 1800 1803 + HaeIII GGCC 1801 1801 . . 1800 1804 + BmgT120I GGNCC 1801 1802 . . 1800 1804 + AsuI* GGNCC 1800 1803 . . 1815 1818 + SetI ASST 1818 1814 . . 1823 1826 + TaqI TCGA 1823 1825 . . 1834 1843 + MslI CAYNNNNRTG 1838 1838 . . 1832 1836 - TspDTI ATGAA 1822 1820 . . 1840 1844 - SfaNI GCATC 1830 1834 . . 1844 1849 + StyI CCWWGG 1844 1848 . . 1844 1849 + SecI* CCNNGG 1844 1848 . . 1851 1855 + BsrI ACTGG 1856 1854 . . 1863 1867 + FokI GGATG 1876 1880 . . 1863 1867 + BseGI GGATG 1869 1867 . . 1865 1870 + EcoT22I ATGCAT 1869 1865 . . 1866 1869 + CviRI* TGCA 1867 1867 . . 1864 1868 - SfaNI GCATC 1854 1858 . . 1915 1919 + HphI GGTGA 1927 1926 . . 1913 1916 - AciI CCGC 1913 1915 . . 1927 1930 + MaeI CTAG 1927 1929 . . 1931 1936 + StuI AGGCCT 1933 1933 . . 1932 1935 + CviJI RGCY 1933 1933 . . 1932 1935 + HaeIII GGCC 1933 1933 . . 1930 1933 - MnlI CCTC 1923 1922 . . 1936 1939 + MseI TTAA 1936 1938 . . 1958 1963 + Hpy178III* TCNNGA 1959 1961 . . 1957 1961 - BslFI GGGAC 1942 1946 . . 1986 1991 + SspI AATATT 1988 1988 . . 1989 1994 + VspI ATTAAT 1990 1992 . . 1990 1993 + MseI TTAA 1990 1992 . . 2001 2006 + Hpy178III* TCNNGA 2002 2004 . . 2001 2006 + BetI* WCCGGW 2001 2005 . . 2001 2006 + BspMII* TCCGGA 2001 2005 . . 2002 2005 + HpaII CCGG 2002 2004 . . 2022 2026 + MboII GAAGA 2034 2033 . . 2033 2038 + SspI AATATT 2035 2035 . . 2039 2042 + TaqI TCGA 2039 2041 . . 2039 2044 + Hpy178III* TCNNGA 2040 2042 . . 2055 2060 + AsuII TTCGAA 2056 2058 . . 2056 2059 + TaqI TCGA 2056 2058 . . 2053 2057 - MboII GAAGA 2045 2044 . . 2061 2066 + ApoI RAATTY 2061 2065 . . 2062 2065 + TspEI AATT 2061 2065 . . 2069 2072 + TspEI AATT 2068 2072 . . 2089 2094 + EciI GGCGGA 2105 2103 . . 2090 2093 - AciI CCGC 2090 2092 . . 2101 2105 + Tsp45I GTSAC 2100 2105 . . 2101 2105 + MaeIII GTNAC 2100 2105 . . 2092 2103 - TstI CACNNNNNNTCC 2116 2111 2084 2079 2132 2135 + MnlI CCTC 2142 2141 . . 2136 2139 + MnlI CCTC 2146 2145 . . 2165 2168 + MseI TTAA 2165 2167 . . 2171 2174 + SetI ASST 2174 2170 . . 2173 2178 + Hpy166II GTNNAC 2175 2175 . . 2183 2187 + TspDTI ATGAA 2198 2196 . . 2186 2189 + TspEI AATT 2185 2189 . . 2189 2192 + CviRI* TGCA 2190 2190 . . 2198 2203 + BclI TGATCA 2198 2202 . . 2199 2202 + DpnI GATC 2200 2200 . . 2199 2202 + BstKTI GATC 2201 2199 . . 2199 2202 + MboI GATC 2198 2202 . . 2207 2210 + FnuDII* CGCG 2208 2208 . . 2210 2214 + HinfI GANTC 2210 2213 . . 2210 2214 - PleI GAGTC 2204 2205 . . 2210 2214 - MlyI GAGTC 2204 2204 . . 2229 2232 + SetI ASST 2232 2228 . . 2236 2239 + FatI CATG 2235 2239 . . 2236 2239 + NlaIII CATG 2239 2235 . . 2236 2239 + CviAII CATG 2236 2238 . . 2234 2238 - TspDTI ATGAA 2224 2222 . . 2256 2260 - Hin4II* CCTTC 2250 2249 . . 2278 2283 + SspI AATATT 2280 2280 . . 2283 2288 + Hpy178III* TCNNGA 2284 2286 . . 2283 2288 + NruI TCGCGA 2285 2285 . . 2284 2287 + FnuDII* CGCG 2285 2285 . . 2287 2291 - Hin4II* CCTTC 2281 2280 . . 2318 2329 + BdaI TGANNNNNNTCA 2307 2305 2341 2339 2319 2328 + BsaBI GATNNNNATC 2323 2323 . . 2318 2329 - BdaI TGANNNNNNTCA 2341 2339 2307 2305 2337 2340 + DpnI GATC 2338 2338 . . 2337 2340 + BstKTI GATC 2339 2337 . . 2337 2340 + MboI GATC 2336 2340 . . 2364 2367 + CviRI* TGCA 2365 2365 . . 2372 2375 + TaiI ACGT 2375 2371 . . 2372 2375 + SetI ASST 2375 2371 . . 2372 2375 + MaeII ACGT 2372 2374 . . 2375 2380 + TsoI TARCCA 2391 2389 . . 2376 2379 + CviJI RGCY 2377 2377 . . 2379 2390 + CspCI CAANNNNNGTGG 2367 2365 2402 2400 2381 2384 + TaiI ACGT 2384 2380 . . 2381 2384 + SetI ASST 2384 2380 . . 2381 2384 + MaeII ACGT 2381 2383 . . 2392 2397 + TatI WGTACW 2392 2396 . . 2392 2397 + ScaI AGTACT 2394 2394 . . 2393 2396 + Csp6I GTAC 2393 2395 . . 2393 2396 + RsaI GTAC 2394 2394 . . 2404 2409 - BsmI GAATGC 2404 2402 . . 2421 2424 + SetI ASST 2424 2420 . . 2421 2424 + CviJI RGCY 2422 2422 . . 2421 2424 + AluI AGCT 2422 2422 . . 2422 2426 + BlsI GCNGC 2424 2423 . . 2422 2426 + BisI GCNGC 2423 2424 . . 2422 2426 + TseI GCWGC 2422 2425 . . 2422 2426 - BbvI GCAGC 2409 2413 . . 2425 2429 - BceAI ACGGC 2410 2412 . . 2432 2437 + ApoI RAATTY 2432 2436 . . 2433 2436 + TspEI AATT 2432 2436 . . 2451 2456 + SplI* CGTACG 2451 2455 . . 2452 2455 + Csp6I GTAC 2452 2454 . . 2452 2455 + RsaI GTAC 2453 2453 . . 2454 2458 + TspGWI ACGGA 2469 2467 . . 2460 2463 + SetI ASST 2463 2459 . . 2465 2470 + Hpy166II GTNNAC 2467 2467 . . 2465 2470 + AccI GTMKAC 2466 2468 . . 2480 2483 + TspEI AATT 2479 2483 . . 2490 2494 + Tsp45I GTSAC 2489 2494 . . 2490 2494 + MaeIII GTNAC 2489 2494 . . 2502 2507 + KpnI GGTACC 2506 2502 . . 2502 2507 + NlaIV GGNNCC 2504 2504 . . 2502 2507 + HgiCI* GGYRCC 2502 2506 . . 2502 2507 + Acc65I GGTACC 2502 2506 . . 2503 2506 + Csp6I GTAC 2503 2505 . . 2499 2503 - BccI CCATC 2493 2494 . . 2503 2506 + RsaI GTAC 2504 2504 . . 2517 2521 + DdeI CTNAG 2517 2520 . . 2517 2521 + BspCNI CTCAG 2530 2528 . . 2517 2521 + BseMII CTCAG 2531 2529 . . 2518 2522 + Hpy188I TCNGA 2520 2519 . . 2523 2526 + SetI ASST 2526 2522 . . 2523 2526 + CviJI RGCY 2524 2524 . . 2523 2526 + AluI AGCT 2524 2524 . . 2528 2531 + MseI TTAA 2528 2530 . . 2531 2535 + TspGWI ACGGA 2546 2544 . . 2534 2538 + TfiI GAWTC 2534 2537 . . 2534 2538 + HinfI GANTC 2534 2537 . . 2541 2545 + FokI GGATG 2554 2558 . . 2541 2545 + BseGI GGATG 2547 2545 . . 2543 2547 + TspDTI ATGAA 2558 2556 . . 2559 2563 + MboII GAAGA 2571 2570 . . 2574 2578 + BsmAI GTCTC 2579 2583 . . 2603 2608 + SecI* CCNNGG 2603 2607 . . 2604 2608 + BssKI CCNGG 2603 2608 . . 2604 2608 + BseBI CCWGG 2605 2606 . . 2604 2608 + ScrFI CCNGG 2605 2606 . . 2604 2608 + EcoRII CCWGG 2603 2608 . . 2608 2611 + Hin6I GCGC 2608 2610 . . 2608 2611 + GlaI GCGC 2609 2609 . . 2608 2611 + HhaI GCGC 2610 2608 . . 2620 2624 - Hin4II* CCTTC 2614 2613 . . 2628 2632 + TfiI GAWTC 2628 2631 . . 2628 2632 + HinfI GANTC 2628 2631 . . 2640 2645 + Cac8I GCNNGC 2642 2642 . . 2646 2651 + Hpy178III* TCNNGA 2647 2649 . . 2647 2652 + Eco57MI CTGRAG 2668 2666 . . 2647 2652 + GsuI CTGGAG 2668 2666 . . 2653 2657 + Tsp4CI* ACNGT 2655 2654 . . 2650 2654 - BsmAI GTCTC 2644 2648 . . 2650 2655 - Eco31I GGTCTC 2644 2648 . . 2657 2662 + Hpy178III* TCNNGA 2658 2660 . . 2663 2666 + MseI TTAA 2663 2665 . . 2670 2674 + HphI GGTGA 2682 2681 . . 2667 2671 - BccI CCATC 2661 2662 . . 2673 2677 + MboII GAAGA 2685 2684 . . 2684 2688 + BslFI GGGAC 2698 2702 . . 2682 2685 - AciI CCGC 2682 2684 . . 2682 2686 - FauI CCCGC 2675 2677 . . 2691 2697 + PasI CCCWGGG 2692 2695 . . 2691 2696 + SecI* CCNNGG 2691 2695 . . 2692 2696 + BssKI CCNGG 2691 2696 . . 2692 2696 + BseBI CCWGG 2693 2694 . . 2692 2697 + SecI* CCNNGG 2692 2696 . . 2692 2696 + ScrFI CCNGG 2693 2694 . . 2692 2696 + EcoRII CCWGG 2691 2696 . . 2697 2700 + Csp6I GTAC 2697 2699 . . 2697 2700 + RsaI GTAC 2698 2698 . . 2703 2708 + AcyI GRCGYC 2704 2706 . . 2704 2708 - HgaI GACGC 2693 2698 . . 2715 2718 + TspEI AATT 2714 2718 . . 2717 2720 + MseI TTAA 2717 2719 . . 2723 2726 + SetI ASST 2726 2722 . . 2721 2725 - Hin4II* CCTTC 2715 2714 . . 2727 2732 + NdeI CATATG 2728 2730 . . 2734 2739 + NlaIV GGNNCC 2736 2736 . . 2734 2739 + HgiCI* GGYRCC 2734 2738 . . 2732 2735 - AciI CCGC 2732 2734 . . 2738 2742 + BssKI CCNGG 2737 2742 . . 2738 2742 + BseBI CCWGG 2739 2740 . . 2738 2743 + SecI* CCNNGG 2738 2742 . . 2738 2742 + ScrFI CCNGG 2739 2740 . . 2738 2742 + EcoRII CCWGG 2737 2742 . . 2742 2747 + NlaIV GGNNCC 2744 2744 . . 2743 2748 + SduI GDGCHC 2747 2743 . . 2743 2748 + HgiJII* GRGCYC 2747 2743 . . 2744 2747 + CviJI RGCY 2745 2745 . . 2745 2750 + Cac8I GCNNGC 2747 2747 . . 2746 2750 + FauI CCCGC 2754 2756 . . 2747 2750 + AciI CCGC 2747 2749 . . 2756 2761 + SfeI* CTRYAG 2756 2760 . . 2774 2777 + TspEI AATT 2773 2777 . . 2816 2819 + TspEI AATT 2815 2819 . . 2830 2835 + SduI GDGCHC 2834 2830 . . 2830 2835 + HgiAI* GWGCWC 2834 2830 . . 2832 2839 + PspXI VCTCGAGB 2833 2837 . . 2832 2842 + MwoI GCNNNNNNNGC 2838 2835 . . 2833 2838 + SmlI CTYRAG 2833 2837 . . 2833 2838 + XhoI CTCGAG 2833 2837 . . 2833 2838 + BmeT110I CYCGRG 2834 2836 . . 2833 2838 + AvaI CYCGRG 2833 2837 . . 2834 2837 + TaqI TCGA 2834 2836 . . 2836 2841 + BseRI GAGGAG 2851 2849 . . 2838 2843 + NlaIV GGNNCC 2840 2840 . . 2836 2839 - MnlI CCTC 2829 2828 . . 2839 2844 + SduI GDGCHC 2843 2839 . . 2839 2844 + HgiJII* GRGCYC 2843 2839 . . 2840 2843 + CviJI RGCY 2841 2841 . . 2844 2850 + TspRI CASTGNN 2850 2841 . . 2847 2850 + CviRI* TGCA 2848 2848 . . 2843 2847 - BsrI ACTGG 2843 2841 . . 2842 2847 - BfiI ACTGGG 2837 2836 . . 2848 2853 + Cac8I GCNNGC 2850 2850 . . 2887 2890 + TspEI AATT 2886 2890 . . 2899 2902 + TaqI TCGA 2899 2901 . . 2904 2908 + FokI GGATG 2917 2921 . . 2904 2908 + BseGI GGATG 2910 2908 . . 2901 2905 - BccI CCATC 2895 2896 . . 2906 2910 + TspDTI ATGAA 2921 2919 . . 2908 2912 + MboII GAAGA 2920 2919 . . 2911 2916 + EcoRI GAATTC 2911 2915 . . 2911 2916 + ApoI RAATTY 2911 2915 . . 2912 2915 + TspEI AATT 2911 2915 . . 2929 2932 + CviRI* TGCA 2930 2930 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 435 2502 # # AccI: # [GTMKAC] # 203 471 2263 # # AciI: # [CCGC] # 15 50 177 190 541 592 # 1372 # # AcyI: # [GRCGYC] # 233 862 1842 # # AflII: # [CTTAAG] # 427 2510 # # AgeI: # [ACCGGT] # 1439 1498 # # AjuI: # [GAANNNNNNNTTGG] # 32 948 1957 # # AluI: # [AGCT] # 102 253 411 413 661 1097 # # AlwNI: # [CAGNNNCTG] # 456 2481 # # ApoI: # [RAATTY] # 15 18 21 26 61 128 # 154 172 178 221 247 308 # 371 479 538 # # AsuI*: # [GGNCC] # 1137 1800 # # AsuII: # [TTCGAA] # 881 898 1158 # # AvaI: # [CYCGRG] # 104 2833 # # BarI: # [GAAGNNNNNNTAC] # 32 1154 1751 # # BbvI: # [GCAGC] # 528 2409 # # BbvII*: # [GAAGAC] # 387 649 661 1240 # # BccI: # [CCATC] # 42 130 139 168 234 406 # 430 1388 # # Bce83I*: # [CTTGAG] # 291 1255 1391 # # BceAI: # [ACGGC] # 527 1080 1330 # # BcgI: # [CGANNNNNNTGC] # 34 34 179 520 2170 # # BclI: # [TGATCA] # 51 411 739 1736 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 550 596 1723 # # BetI*: # [WCCGGW] # 562 936 1439 # # BfiI: # [ACTGGG] # 100 2837 # # BglII: # [AGATCT] # 222 321 373 2021 # # BinI*: # [GGATC] # 204 2733 # # BisI: # [GCNGC] # 514 2423 # # BlsI: # [GCNGC] # 513 2424 # # BmeT110I: # [CYCGRG] # 103 2834 # # BmgT120I: # [GGNCC] # 1136 1801 # # BsaBI: # [GATNNNNATC] # 614 921 1402 # # BsaXI: # [ACNNNNNCTCC] # 30 98 2809 # # BseBI: # [CCWGG] # 10 46 88 198 285 1025 # 1285 # # BseGI: # [GGATG] # 27 84 339 363 678 1446 # # BseMII: # [CTCAG] # 398 404 406 1729 # # BseRI: # [GAGGAG] # 3 86 116 2732 # # BsiYI*: # [CCNNNNNNNGG] # 646 723 1568 # # BslFI: # [GGGAC] # 239 756 849 1093 # # BsmAI: # [GTCTC] # 65 293 791 1788 # # BsmI: # [GAATGC] # 533 2404 # # BspCNI: # [CTCAG] # 399 402 407 1729 # # BspMII*: # [TCCGGA] # 936 2001 # # BsrDI: # [GCAATG] # 437 2500 # # BsrI: # [ACTGG] # 94 534 640 682 987 # # BssKI: # [CCNGG] # 10 46 88 200 285 1023 # 1285 # # BstKTI: # [GATC] # 9 15 37 54 103 121 # 138 222 358 411 598 871 # # BtrI: # [CACGTC] # 1189 1748 # # Cac8I: # [GCNNGC] # 27 87 103 105 528 993 # 1094 # # Cfr10I: # [RCCGGY] # 1439 1498 # # CfrI: # [YGGCCR] # 1065 1872 # # ClaI: # [ATCGAT] # 488 2449 # # Csp6I: # [GTAC] # 36 51 59 84 194 217 # 240 300 1756 # # CspCI: # [CAANNNNNGTGG] # 35 535 2367 # # CviAII: # [CATG] # 214 284 313 689 701 736 # # CviJI: # [RGCY] # 4 15 15 32 40 45 # 45 52 93 96 96 102 # 132 221 238 243 314 321 # 389 444 # # CviRI*: # [TGCA] # 7 69 72 79 82 84 # 129 149 175 219 291 323 # 333 442 483 # # DdeI: # [CTNAG] # 381 407 420 1729 # # DpnI: # [GATC] # 9 15 37 53 103 121 # 138 222 358 411 599 871 # # Eam1105I: # [GACNNNNNGTC] # 1437 1500 # # EciI: # [GGCGGA] # 832 2105 # # Eco31I: # [GGTCTC] # 293 2644 # # Eco57I: # [CTGAAG] # 950 1987 # # Eco57MI: # [CTGRAG] # 269 950 1718 # # EcoP15I: # [CAGCAG] # 376 2561 # # EcoRI: # [GAATTC] # 26 1313 1598 # # EcoRII: # [CCWGG] # 10 46 88 200 285 1023 # 1285 # # EcoT22I: # [ATGCAT] # 149 528 1068 1192 # # Esp3I: # [CGTCTC] # 791 2146 # # EspI*: # [GCTNAGC] # 407 2530 # # FatI: # [CATG] # 213 284 313 689 702 736 # # FauI: # [CCCGC] # 79 183 2675 # # FnuDII*: # [CGCG] # 77 652 2208 # # FokI: # [GGATG] # 20 84 326 363 678 1466 # # GlaI: # [GCGC] # 319 328 875 1415 # # GsuI: # [CTGGAG] # 269 2668 # # HaeII: # [RGCGCY] # 1196 1741 # # HaeIII: # [GGCC] # 132 278 318 456 749 1004 # # HgaI: # [GACGC] # 244 851 1842 # # HgiAI*: # [GWGCWC] # 103 2834 # # HgiCI*: # [GGYRCC] # 203 232 2502 # # HgiJII*: # [GRGCYC] # 94 96 749 1998 # # HhaI: # [GCGC] # 319 327 876 1415 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 424 1030 # 1419 # # Hin4II*: # [CCTTC] # 31 101 147 147 222 333 # 560 584 812 # # Hin6I: # [GCGC] # 319 329 874 1415 # # HindII: # [GTYRAC] # 259 803 1875 # # HindIII: # [AAGCTT] # 1431 1506 # # HinfI: # [GANTC] # 12 57 84 94 123 141 # 224 239 309 324 372 466 # 492 # # HpaII: # [CCGG] # 562 935 1440 # # HphI: # [GGTGA] # 145 169 194 255 512 755 # 907 # # Hpy166II: # [GTNNAC] # 41 204 218 292 470 599 # 1113 # # Hpy178III*: # [TCNNGA] # 11 38 43 92 144 244 # 279 319 363 400 1004 # # Hpy188I: # [TCNGA] # 9 12 13 24 88 122 # 168 194 195 258 269 333 # 417 835 # # KpnI: # [GGTACC] # 431 2506 # # Ksp632I*: # [CTCTTC] # 142 149 243 251 439 576 # 1137 # # MaeI: # [CTAG] # 195 514 1010 1218 # # MaeII: # [ACGT] # 9 35 113 245 326 389 # 406 556 858 # # MaeIII: # [GTNAC] # 183 281 389 448 639 997 # # MboI: # [GATC] # 9 15 37 51 103 121 # 138 222 358 411 601 871 # # MboII: # [GAAGA] # 3 11 17 23 30 43 # 69 73 82 90 95 97 # 114 123 155 235 247 247 # 248 409 526 # # McrI*: # [CGRYCG] # 1068 1869 # # MfeI: # [CAATTG] # 557 920 1460 # # MlyI: # [GAGTC] # 218 254 372 733 1360 # # MmeI: # [TCCRAC] # 426 2511 # # MnlI: # [CCTC] # 3 4 28 39 41 53 # 54 78 108 120 150 152 # 180 219 267 363 395 683 # # MseI: # [TTAA] # 28 54 54 63 97 135 # 164 173 175 220 309 350 # 363 752 # # MslI: # [CAYNNNNRTG] # 1099 1838 # # MwoI: # [GCNNNNNNNGC] # 99 1211 1627 # # NdeI: # [CATATG] # 209 2728 # # NlaIII: # [CATG] # 217 284 313 689 698 736 # # NlaIV: # [GGNNCC] # 8 96 97 232 599 1905 # # NmeAIII: # [GCCGAG] # 1093 1844 # # NruI: # [TCGCGA] # 652 2285 # # NspI: # [RCATGY] # 530 1020 1387 # # PasI: # [CCCWGGG] # 245 1319 1373 # # PflMI: # [CCANNNNNTGG] # 646 2291 # # PleI: # [GAGTC] # 218 253 372 733 1361 # # PspXI: # [VCTCGAGB] # 104 2833 # # PstI: # [CTGCAG] # 756 2181 # # RsaI: # [GTAC] # 36 51 59 84 194 217 # 239 301 1756 # # SapI: # [GCTCTTC] # 1380 1557 # # ScaI: # [AGTACT] # 543 2394 # # ScrFI: # [CCNGG] # 10 46 88 198 285 1025 # 1285 # # SduI: # [GDGCHC] # 9 87 94 749 1998 # # SecI*: # [CCNNGG] # 1 1 46 88 199 294 # 525 759 1024 # # SetI: # [ASST] # 7 9 15 23 32 35 # 39 40 44 52 58 63 # 63 86 109 116 120 134 # 136 138 143 165 200 211 # 246 297 356 # # SfaNI: # [GCATC] # 24 662 1083 1168 # # SfeI*: # [CTRYAG] # 135 162 181 222 752 1485 # # SmlI: # [CTYRAG] # 104 157 270 1098 1308 # # SphI: # [GCATGC] # 530 1020 1387 # # SplI*: # [CGTACG] # 486 2451 # # SspI: # [AATATT] # 47 245 312 657 1676 # # StuI: # [AGGCCT] # 410 1004 1523 # # StyI: # [CCWWGG] # 1093 1844 # # TaiI: # [ACGT] # 9 35 116 245 326 389 # 406 553 858 # # TaqI: # [TCGA] # 17 38 65 155 216 255 # 488 778 925 # # TatI: # [WGTACW] # 299 545 2093 # # TfiI: # [GAWTC] # 12 84 94 123 141 309 # 492 790 892 # # TseI: # [GCWGC] # 515 2422 # # TsoI: # [TARCCA] # 546 2391 # # Tsp45I: # [GTSAC] # 183 281 389 448 1636 # # Tsp4CI*: # [ACNGT] # 28 194 271 282 486 717 # 959 # # TspDTI: # [ATGAA] # 16 21 24 26 33 113 # 131 138 184 203 271 278 # 334 363 376 426 # # TspEI: # [AATT] # 7 9 11 13 14 15 # 18 21 25 26 32 33 # 39 42 47 47 50 59 # 71 84 84 95 96 117 # 128 133 139 140 177 182 # 193 235 247 308 # # TspGWI: # [ACGGA] # 77 96 202 391 1012 1159 # # TspRI: # [CASTGNN] # 87 647 2203 # # TstI: # [CACNNNNNNTCC] # 32 821 2084 # # VspI: # [ATTAAT] # 947 1990 # # XcmI: # [CCANNNNNNNNNTGG] # 467 2470 # # XhoI: # [CTCGAG] # 104 2833 # # XhoII: # [RGATCY] # 222 321 373 2021 # # XmaIII*: # [CGGCCG] # 1065 1872 # # XmnI: # [GAANNNNTTC] # 1366 1571 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2937 # Reported_sequences: 1 # Reported_hitcount: 618 #---------------------------------------