Restriction Fragments for SFP1/YLR403W



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The currently selected gene/sequence is :

SFP1/YLR403W on chromosome XII from coordinates 925568 to 927619.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22836.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 25 30 + Cac8I GCNNGC 27 27 . . 36 39 + CviJI RGCY 37 37 . . 35 40 - TsoI TARCCA 25 23 . . 40 45 + SpeI ACTAGT 40 44 . . 41 44 + MaeI CTAG 41 43 . . 43 48 + TatI WGTACW 43 47 . . 43 48 + ScaI AGTACT 45 45 . . 44 47 + Csp6I GTAC 44 46 . . 44 47 + RsaI GTAC 45 45 . . 53 57 + Hpy99I CGWCG 57 52 . . 55 59 + BceAI ACGGC 71 73 . . 57 60 + CviJI RGCY 58 58 . . 58 68 + MwoI GCNNNNNNNGC 64 61 . . 69 74 + NspI RCATGY 73 69 . . 70 73 + FatI CATG 69 73 . . 70 73 + NlaIII CATG 73 69 . . 70 73 + CviAII CATG 70 72 . . 71 76 + EcoT22I ATGCAT 75 71 . . 72 75 + CviRI* TGCA 73 73 . . 73 77 + SfaNI GCATC 82 86 . . 81 86 + ApoI RAATTY 81 85 . . 82 85 + TspEI AATT 81 85 . . 98 101 + MseI TTAA 98 100 . . 100 105 + SspI AATATT 102 102 . . 112 116 + TfiI GAWTC 112 115 . . 112 116 + HinfI GANTC 112 115 . . 114 119 + AsuII TTCGAA 115 117 . . 115 118 + TaqI TCGA 115 117 . . 130 134 + Hin4II* CCTTC 140 139 . . 151 154 + TspEI AATT 150 154 . . 154 158 + Hpy188I TCNGA 156 155 . . 157 161 + TfiI GAWTC 157 160 . . 157 161 + HinfI GANTC 157 160 . . 160 164 - MboII GAAGA 152 151 . . 185 188 + MseI TTAA 185 187 . . 188 192 + BceAI ACGGC 204 206 . . 190 195 + AcyI GRCGYC 191 193 . . 191 195 - HgaI GACGC 180 185 . . 199 204 + SspI AATATT 201 201 . . 204 208 - HphI GGTGA 196 195 . . 218 223 + Hpy178III* TCNNGA 219 221 . . 218 223 + BspHI TCATGA 218 222 . . 219 222 + FatI CATG 218 222 . . 219 222 + NlaIII CATG 222 218 . . 219 222 + CviAII CATG 219 221 . . 220 224 + TspDTI ATGAA 235 233 . . 239 243 - TspDTI ATGAA 229 227 . . 245 250 + TatI WGTACW 245 249 . . 246 249 + Csp6I GTAC 246 248 . . 246 249 + RsaI GTAC 247 247 . . 264 268 + BmgT120I GGNCC 265 266 . . 264 268 + AvaII GGWCC 264 267 . . 264 268 + AsuI* GGNCC 264 267 . . 291 296 - BsrDI GCAATG 290 288 . . 296 306 + BsiYI* CCNNNNNNNGG 302 299 . . 300 304 + MaeIII GTNAC 299 304 . . 303 307 + TspGWI ACGGA 318 316 . . 310 314 + TfiI GAWTC 310 313 . . 310 314 + HinfI GANTC 310 313 . . 318 321 + CviJI RGCY 319 319 . . 326 331 + SmlI CTYRAG 326 330 . . 326 331 - Bce83I* CTTGAG 311 309 . . 332 336 + FokI GGATG 345 349 . . 332 336 + BseGI GGATG 338 336 . . 333 337 - BccI CCATC 327 328 . . 335 340 - TaqII CACCCA 325 323 . . 332 343 - TstI CACNNNNNNTCC 356 351 324 319 347 350 + FatI CATG 346 350 . . 347 350 + NlaIII CATG 350 346 . . 347 350 + CviAII CATG 347 349 . . 345 349 - TspDTI ATGAA 335 333 . . 352 357 + XhoII RGATCY 352 356 . . 352 357 + NlaIV GGNNCC 354 354 . . 352 357 + BamHI GGATCC 352 356 . . 352 356 + BinI* GGATC 360 361 . . 353 356 + DpnI GATC 354 354 . . 353 356 + BstKTI GATC 355 353 . . 353 356 + MboI GATC 352 356 . . 353 357 - BinI* GGATC 347 348 . . 376 379 + MseI TTAA 376 378 . . 373 378 - TsoI TARCCA 363 361 . . 383 387 + TspDTI ATGAA 398 396 . . 387 390 + TspEI AATT 386 390 . . 394 397 + CviJI RGCY 395 395 . . 395 399 - BceAI ACGGC 380 382 . . 412 415 + Csp6I GTAC 412 414 . . 412 415 + RsaI GTAC 413 413 . . 414 417 + SetI ASST 417 413 . . 414 419 + BspMI ACCTGC 423 427 . . 417 420 + CviRI* TGCA 418 418 . . 418 422 + SfaNI GCATC 427 431 . . 424 434 + MwoI GCNNNNNNNGC 430 427 . . 424 434 + BstAPI GCANNNNNTGC 430 427 . . 428 432 + Tsp4CI* ACNGT 430 429 . . 433 443 + MwoI GCNNNNNNNGC 439 436 . . 433 437 + BlsI GCNGC 435 434 . . 433 437 + BisI GCNGC 434 435 . . 433 437 + TseI GCWGC 433 436 . . 436 446 + MwoI GCNNNNNNNGC 442 439 . . 437 440 + MnlI CCTC 447 446 . . 433 437 - BbvI GCAGC 420 424 . . 440 445 + NspBII* CMGCKG 442 442 . . 440 443 + AciI CCGC 440 442 . . 442 446 + BlsI GCNGC 444 443 . . 442 446 + BisI GCNGC 443 444 . . 442 446 + TseI GCWGC 442 445 . . 445 449 + TauI GCSGC 448 445 . . 445 455 + MwoI GCNNNNNNNGC 451 448 . . 445 449 + BlsI GCNGC 447 446 . . 445 449 + BisI GCNGC 446 447 . . 442 446 - BbvI GCAGC 429 433 . . 446 449 + AciI CCGC 446 448 . . 453 456 + CviRI* TGCA 454 454 . . 479 482 + CviRI* TGCA 480 480 . . 483 487 + TfiI GAWTC 483 486 . . 483 487 + HinfI GANTC 483 486 . . 497 501 + DdeI CTNAG 497 500 . . 501 505 - BsmAI GTCTC 495 499 . . 506 509 + MnlI CCTC 516 515 . . 501 506 - Eco31I GGTCTC 495 499 . . 520 523 + AciI CCGC 520 522 . . 519 524 - EciI GGCGGA 509 507 . . 533 536 - MnlI CCTC 526 525 . . 550 553 + MnlI CCTC 560 559 . . 551 555 + DdeI CTNAG 551 554 . . 551 555 + BspCNI CTCAG 564 562 . . 551 555 + BseMII CTCAG 565 563 . . 552 556 + Hpy188I TCNGA 554 553 . . 548 553 - BseRI GAGGAG 539 537 . . 558 561 + CviJI RGCY 559 559 . . 559 569 + MwoI GCNNNNNNNGC 565 562 . . 564 570 + TspRI CASTGNN 570 561 . . 564 569 - BtsI GCAGTG 563 561 . . 582 587 + XbaI TCTAGA 582 586 . . 582 587 + Hpy178III* TCNNGA 583 585 . . 583 586 + MaeI CTAG 583 585 . . 593 598 + SspI AATATT 595 595 . . 611 614 + FatI CATG 610 614 . . 611 614 + NlaIII CATG 614 610 . . 611 614 + CviAII CATG 611 613 . . 609 613 - TspDTI ATGAA 599 597 . . 630 636 + SexAI ACCWGGT 630 635 . . 631 635 + BssKI CCNGG 630 635 . . 631 635 + BseBI CCWGG 632 633 . . 631 635 + ScrFI CCNGG 632 633 . . 631 635 + EcoRII CCWGG 630 635 . . 633 636 + SetI ASST 636 632 . . 638 641 + CviRI* TGCA 639 639 . . 639 649 + MwoI GCNNNNNNNGC 645 642 . . 639 649 + BstAPI GCANNNNNTGC 645 642 . . 640 643 + FatI CATG 639 643 . . 640 643 + NlaIII CATG 643 639 . . 640 643 + CviAII CATG 640 642 . . 649 653 + DdeI CTNAG 649 652 . . 649 653 - BspCNI CTCAG 641 639 . . 649 653 - BseMII CTCAG 640 638 . . 651 655 - BsmAI GTCTC 645 649 . . 661 665 + MboII GAAGA 673 672 . . 666 669 + SetI ASST 669 665 . . 666 669 + CviJI RGCY 667 667 . . 666 669 + AluI AGCT 667 667 . . 678 683 + BtrI CACGTC 680 680 . . 679 682 + TaiI ACGT 682 678 . . 679 682 + SetI ASST 682 678 . . 679 682 + MaeII ACGT 679 681 . . 680 685 + Esp3I CGTCTC 686 690 . . 681 685 + BsmAI GTCTC 686 690 . . 702 706 + Tsp45I GTSAC 701 706 . . 702 706 + MaeIII GTNAC 701 706 . . 715 719 + TspDTI ATGAA 730 728 . . 722 727 - EcoP15I CAGCAG 694 696 . . 728 732 + MaeIII GTNAC 727 732 . . 733 738 + SpeI ACTAGT 733 737 . . 734 737 + MaeI CTAG 734 736 . . 728 740 - PsrI GAACNNNNNNTAC 752 747 720 715 738 742 - TspDTI ATGAA 728 726 . . 763 766 + SetI ASST 766 762 . . 764 767 + MnlI CCTC 774 773 . . 765 770 + Ksp632I* CTCTTC 771 774 . . 766 770 - MboII GAAGA 758 757 . . 782 785 + SetI ASST 785 781 . . 782 787 + BspMI ACCTGC 791 795 . . 784 789 + PstI CTGCAG 788 784 . . 784 789 + SfeI* CTRYAG 784 788 . . 785 788 + CviRI* TGCA 786 786 . . 795 798 + FatI CATG 794 798 . . 795 798 + NlaIII CATG 798 794 . . 795 798 + CviAII CATG 795 797 . . 797 802 + CfrI YGGCCR 797 801 . . 798 801 + CviJI RGCY 799 799 . . 798 801 + HaeIII GGCC 799 799 . . 799 803 + TauI GCSGC 802 799 . . 799 803 + BlsI GCNGC 801 800 . . 799 803 + BisI GCNGC 800 801 . . 800 803 + AciI CCGC 800 802 . . 816 819 + CviRI* TGCA 817 817 . . 819 822 + SetI ASST 822 818 . . 817 822 - BspMI ACCTGC 808 812 . . 839 842 + CviRI* TGCA 840 840 . . 840 845 + Cac8I GCNNGC 842 842 . . 843 846 + CviJI RGCY 844 844 . . 851 855 + BlsI GCNGC 853 852 . . 851 855 + BisI GCNGC 852 853 . . 851 855 + BbvI GCAGC 863 867 . . 851 855 + TseI GCWGC 851 854 . . 854 859 + BsrDI GCAATG 861 859 . . 858 861 + CviRI* TGCA 859 859 . . 871 874 + SetI ASST 874 870 . . 872 875 + MnlI CCTC 882 881 . . 877 882 + VspI ATTAAT 878 880 . . 878 881 + MseI TTAA 878 880 . . 886 891 + EcoT22I ATGCAT 890 886 . . 887 890 + CviRI* TGCA 888 888 . . 888 893 + NspI RCATGY 892 888 . . 888 893 + Cac8I GCNNGC 890 890 . . 888 893 + SphI GCATGC 892 888 . . 889 892 + FatI CATG 888 892 . . 889 892 + NlaIII CATG 892 888 . . 889 892 + CviAII CATG 889 891 . . 900 904 + Hpy188I TCNGA 902 901 . . 897 901 - MboII GAAGA 889 888 . . 898 903 - Eco57MI CTGRAG 883 881 . . 898 903 - Eco57I CTGAAG 883 881 . . 909 912 + FatI CATG 908 912 . . 909 912 + NlaIII CATG 912 908 . . 909 912 + CviAII CATG 909 911 . . 913 917 + TfiI GAWTC 913 916 . . 913 917 + HinfI GANTC 913 916 . . 913 923 + Hin4I GAYNNNNNVTC 904 899 936 931 916 921 + MmeI TCCRAC 941 939 . . 938 942 + DdeI CTNAG 938 941 . . 938 942 + BspCNI CTCAG 951 949 . . 938 942 + BseMII CTCAG 952 950 . . 942 946 + SfaNI GCATC 951 955 . . 946 949 + MnlI CCTC 956 955 . . 943 947 - FokI GGATG 929 933 . . 943 947 - BseGI GGATG 942 940 . . 951 955 + BccI CCATC 959 960 . . 948 952 - HphI GGTGA 940 939 . . 955 960 + EcoP15I CAGCAG 985 987 . . 975 979 + Tsp4CI* ACNGT 977 976 . . 980 983 + CviRI* TGCA 981 981 . . 985 988 + TaqI TCGA 985 987 . . 987 992 + EcoRI GAATTC 987 991 . . 987 992 + ApoI RAATTY 987 991 . . 988 991 + TspEI AATT 987 991 . . 1007 1015 + AlwNI CAGNNNCTG 1012 1009 . . 1011 1014 + CviJI RGCY 1012 1012 . . 1015 1018 + Csp6I GTAC 1015 1017 . . 1015 1018 + RsaI GTAC 1016 1016 . . 1022 1030 + DraIII CACNNNGTG 1027 1024 . . 1040 1048 + AlwNI CAGNNNCTG 1045 1042 . . 1049 1053 + Hpy99I CGWCG 1053 1048 . . 1048 1052 - HgaI GACGC 1037 1042 . . 1053 1058 + Hpy166II GTNNAC 1055 1055 . . 1042 1053 - BcgI CGANNNNNNTGC 1065 1063 1031 1029 1062 1065 + CviRI* TGCA 1063 1063 . . 1077 1082 + EcoRV GATATC 1079 1079 . . 1090 1095 + NlaIV GGNNCC 1092 1092 . . 1090 1095 + HgiCI* GGYRCC 1090 1094 . . 1091 1096 + SduI GDGCHC 1095 1091 . . 1091 1096 + BseSI GKGCMC 1095 1091 . . 1095 1105 + BsiYI* CCNNNNNNNGG 1101 1098 . . 1096 1106 + BsiYI* CCNNNNNNNGG 1102 1099 . . 1101 1106 + BmeT110I CYCGRG 1102 1104 . . 1101 1106 + AvaI CYCGRG 1101 1105 . . 1105 1108 + CviJI RGCY 1106 1106 . . 1116 1119 + CviJI RGCY 1117 1117 . . 1115 1120 - TsoI TARCCA 1105 1103 . . 1138 1147 + MslI CAYNNNNRTG 1142 1142 . . 1140 1144 + Hpy188I TCNGA 1142 1141 . . 1153 1156 + MaeI CTAG 1153 1155 . . 1157 1160 + TaqI TCGA 1157 1159 . . 1159 1163 - SfaNI GCATC 1149 1153 . . 1162 1166 - BceAI ACGGC 1147 1149 . . 1186 1189 + MseI TTAA 1186 1188 . . 1194 1204 + BplI GAGNNNNNCTC 1185 1180 1217 1212 1198 1201 + TspEI AATT 1197 1201 . . 1194 1204 - BplI GAGNNNNNCTC 1217 1212 1185 1180 1204 1208 + BccI CCATC 1212 1213 . . 1207 1212 + Hpy178III* TCNNGA 1208 1210 . . 1212 1217 + AflIII ACRYGT 1212 1216 . . 1214 1217 + TaiI ACGT 1217 1213 . . 1214 1217 + SetI ASST 1217 1213 . . 1214 1217 + MaeII ACGT 1214 1216 . . 1219 1222 + AciI CCGC 1219 1221 . . 1233 1236 + TspEI AATT 1232 1236 . . 1235 1238 + MseI TTAA 1235 1237 . . 1238 1242 + Tsp4CI* ACNGT 1240 1239 . . 1241 1245 + MaeIII GTNAC 1240 1245 . . 1245 1256 + CspCI CAANNNNNGTGG 1233 1231 1268 1266 1276 1279 + SetI ASST 1279 1275 . . 1277 1280 + MnlI CCTC 1287 1286 . . 1285 1288 + TspEI AATT 1284 1288 . . 1291 1296 + SspI AATATT 1293 1293 . . 1295 1298 + MseI TTAA 1295 1297 . . 1299 1302 + FatI CATG 1298 1302 . . 1299 1302 + NlaIII CATG 1302 1298 . . 1299 1302 + CviAII CATG 1299 1301 . . 1316 1321 + DsaI* CCRYGG 1316 1320 . . 1316 1321 + StyI CCWWGG 1316 1320 . . 1316 1321 + NcoI CCATGG 1316 1320 . . 1316 1321 + SecI* CCNNGG 1316 1320 . . 1317 1320 + FatI CATG 1316 1320 . . 1317 1320 + NlaIII CATG 1320 1316 . . 1317 1320 + CviAII CATG 1317 1319 . . 1320 1324 + HphI GGTGA 1332 1331 . . 1323 1327 + TfiI GAWTC 1323 1326 . . 1323 1327 + HinfI GANTC 1323 1326 . . 1328 1333 + Hpy166II GTNNAC 1330 1330 . . 1340 1343 + MseI TTAA 1340 1342 . . 1356 1368 + AloI GAACNNNNNNTCC 1348 1343 1380 1375 1363 1366 + TaiI ACGT 1366 1362 . . 1363 1366 + SetI ASST 1366 1362 . . 1363 1366 + MaeII ACGT 1363 1365 . . 1366 1372 + PfoI TCCNGGA 1366 1371 . . 1367 1371 + BssKI CCNGG 1366 1371 . . 1367 1371 + BseBI CCWGG 1368 1369 . . 1367 1371 + ScrFI CCNGG 1368 1369 . . 1367 1371 + EcoRII CCWGG 1366 1371 . . 1371 1375 + TfiI GAWTC 1371 1374 . . 1371 1375 + HinfI GANTC 1371 1374 . . 1390 1395 + Hpy178III* TCNNGA 1391 1393 . . 1398 1401 + TspEI AATT 1397 1401 . . 1401 1412 + BdaI TGANNNNNNTCA 1390 1388 1424 1422 1402 1405 + DpnI GATC 1403 1403 . . 1402 1405 + BstKTI GATC 1404 1402 . . 1402 1411 + BsaBI GATNNNNATC 1406 1406 . . 1402 1405 + MboI GATC 1401 1405 . . 1405 1408 + MnlI CCTC 1415 1414 . . 1402 1406 - BinI* GGATC 1396 1397 . . 1401 1412 - BdaI TGANNNNNNTCA 1424 1422 1390 1388 1425 1428 + SetI ASST 1428 1424 . . 1426 1429 + MnlI CCTC 1436 1435 . . 1423 1427 - HphI GGTGA 1415 1414 . . 1432 1435 + Csp6I GTAC 1432 1434 . . 1432 1435 + RsaI GTAC 1433 1433 . . 1453 1456 + MseI TTAA 1453 1455 . . 1464 1468 + FokI GGATG 1477 1481 . . 1464 1468 + BseGI GGATG 1470 1468 . . 1479 1485 + DraII RGGNCCY 1480 1483 . . 1479 1484 + SduI GDGCHC 1483 1479 . . 1479 1484 + Bsp120I GGGCCC 1479 1483 . . 1479 1484 + NlaIV GGNNCC 1481 1481 . . 1479 1483 + BmgT120I GGNCC 1480 1481 . . 1479 1484 + HgiJII* GRGCYC 1483 1479 . . 1479 1484 + BseSI GKGCMC 1483 1479 . . 1479 1483 + AsuI* GGNCC 1479 1482 . . 1479 1484 + ApaI GGGCCC 1483 1479 . . 1480 1483 + CviJI RGCY 1481 1481 . . 1480 1483 + HaeIII GGCC 1481 1481 . . 1480 1484 + BmgT120I GGNCC 1481 1482 . . 1480 1484 + AsuI* GGNCC 1480 1483 . . 1484 1487 + MaeI CTAG 1484 1486 . . 1487 1491 + MaeIII GTNAC 1486 1491 . . 1488 1493 + TsoI TARCCA 1504 1502 . . 1495 1498 + TspEI AATT 1494 1498 . . 1500 1503 + FatI CATG 1499 1503 . . 1500 1503 + NlaIII CATG 1503 1499 . . 1500 1503 + CviAII CATG 1500 1502 . . 1501 1505 + TspDTI ATGAA 1516 1514 . . 1518 1523 + MmeI TCCRAC 1543 1541 . . 1519 1529 + BsiYI* CCNNNNNNNGG 1525 1522 . . 1523 1528 + SfeI* CTRYAG 1523 1527 . . 1527 1530 + SetI ASST 1530 1526 . . 1519 1532 - AjuI GAANNNNNNNTTGG 1544 1539 1512 1507 1550 1554 - TspDTI ATGAA 1540 1538 . . 1555 1559 - MboII GAAGA 1547 1546 . . 1561 1566 + Hpy166II GTNNAC 1563 1563 . . 1561 1564 + Csp6I GTAC 1561 1563 . . 1561 1564 + RsaI GTAC 1562 1562 . . 1558 1562 - TspGWI ACGGA 1548 1546 . . 1573 1577 - MboII GAAGA 1565 1564 . . 1584 1587 + FatI CATG 1583 1587 . . 1584 1587 + NlaIII CATG 1587 1583 . . 1584 1587 + CviAII CATG 1584 1586 . . 1589 1592 + TaqI TCGA 1589 1591 . . 1593 1597 + BinI* GGATC 1601 1602 . . 1591 1594 - MnlI CCTC 1584 1583 . . 1594 1597 + DpnI GATC 1595 1595 . . 1594 1597 + BstKTI GATC 1596 1594 . . 1594 1597 + MboI GATC 1593 1597 . . 1596 1601 + Hpy178III* TCNNGA 1597 1599 . . 1675 1678 + CviJI RGCY 1676 1676 . . 1675 1680 + NlaIV GGNNCC 1677 1677 . . 1677 1682 - Eco57MI CTGRAG 1662 1660 . . 1677 1682 - GsuI CTGGAG 1662 1660 . . 1679 1683 - BsrI ACTGG 1679 1677 . . 1690 1702 - AloI GAACNNNNNNTCC 1714 1709 1682 1677 1700 1704 - TspDTI ATGAA 1690 1688 . . 1711 1714 + TspEI AATT 1710 1714 . . 1723 1726 + MnlI CCTC 1733 1732 . . 1719 1723 - SfaNI GCATC 1709 1713 . . 1723 1729 + Bpu10I CCTNAGC 1724 1727 . . 1723 1729 + BbvCI CCTCAGC 1724 1727 . . 1724 1728 + DdeI CTNAG 1724 1727 . . 1724 1728 + BspCNI CTCAG 1737 1735 . . 1724 1728 + BseMII CTCAG 1738 1736 . . 1728 1733 + Cac8I GCNNGC 1730 1730 . . 1730 1735 + HindIII AAGCTT 1730 1734 . . 1731 1734 + SetI ASST 1734 1730 . . 1731 1734 + CviJI RGCY 1732 1732 . . 1731 1734 + AluI AGCT 1732 1732 . . 1744 1747 + DpnI GATC 1745 1745 . . 1744 1747 + BstKTI GATC 1746 1744 . . 1744 1747 + MboI GATC 1743 1747 . . 1744 1748 - BinI* GGATC 1738 1739 . . 1757 1760 + CviJI RGCY 1758 1758 . . 1754 1758 - Hin4II* CCTTC 1748 1747 . . 1770 1774 + BinI* GGATC 1778 1779 . . 1771 1774 + DpnI GATC 1772 1772 . . 1771 1774 + BstKTI GATC 1773 1771 . . 1771 1774 + MboI GATC 1770 1774 . . 1773 1778 + Hpy178III* TCNNGA 1774 1776 . . 1773 1778 + BspHI TCATGA 1773 1777 . . 1774 1777 + FatI CATG 1773 1777 . . 1774 1777 + NlaIII CATG 1777 1773 . . 1774 1777 + CviAII CATG 1774 1776 . . 1777 1780 - MnlI CCTC 1770 1769 . . 1793 1796 + MseI TTAA 1793 1795 . . 1801 1811 + BsiYI* CCNNNNNNNGG 1807 1804 . . 1801 1811 + PflMI CCANNNNNTGG 1807 1804 . . 1801 1805 - BsrI ACTGG 1801 1799 . . 1824 1829 + PsiI TTATAA 1826 1826 . . 1838 1842 + Tsp4CI* ACNGT 1840 1839 . . 1848 1851 + Csp6I GTAC 1848 1850 . . 1848 1851 + RsaI GTAC 1849 1849 . . 1855 1858 + SetI ASST 1858 1854 . . 1859 1862 + CviRI* TGCA 1860 1860 . . 1862 1866 + TspGWI ACGGA 1877 1875 . . 1869 1873 + Hpy188I TCNGA 1871 1870 . . 1872 1876 + TfiI GAWTC 1872 1875 . . 1872 1876 + HinfI GANTC 1872 1875 . . 1880 1883 + TspEI AATT 1879 1883 . . 1885 1888 + FatI CATG 1884 1888 . . 1885 1888 + NlaIII CATG 1888 1884 . . 1885 1888 + CviAII CATG 1885 1887 . . 1886 1890 + TspDTI ATGAA 1901 1899 . . 1898 1902 + Tsp4CI* ACNGT 1900 1899 . . 1901 1904 + Csp6I GTAC 1901 1903 . . 1901 1904 + RsaI GTAC 1902 1902 . . 1917 1922 + XhoII RGATCY 1917 1921 . . 1918 1921 + DpnI GATC 1919 1919 . . 1918 1921 + BstKTI GATC 1920 1918 . . 1918 1921 + MboI GATC 1917 1921 . . 1920 1925 + Hpy178III* TCNNGA 1921 1923 . . 1918 1922 - BinI* GGATC 1912 1913 . . 1924 1928 + TfiI GAWTC 1924 1927 . . 1924 1928 + HinfI GANTC 1924 1927 . . 1927 1930 + TaqI TCGA 1927 1929 . . 1933 1937 + TfiI GAWTC 1933 1936 . . 1933 1937 + HinfI GANTC 1933 1936 . . 1942 1946 + HphI GGTGA 1954 1953 . . 1945 1949 - BccI CCATC 1939 1940 . . 1953 1958 + SduI GDGCHC 1957 1953 . . 1953 1958 + HgiJII* GRGCYC 1957 1953 . . 1954 1957 + CviJI RGCY 1955 1955 . . 1959 1962 + CviRI* TGCA 1960 1960 . . 1976 1980 + Tsp4CI* ACNGT 1978 1977 . . 2015 2019 + Tsp4CI* ACNGT 2017 2016 . . 2019 2024 + AhaIII* TTTAAA 2021 2021 . . 2020 2023 + MseI TTAA 2020 2022 . . 2033 2038 + NlaIV GGNNCC 2035 2035 . . 2034 2038 + BmgT120I GGNCC 2035 2036 . . 2034 2038 + AsuI* GGNCC 2034 2037 . . 2035 2038 + CviJI RGCY 2036 2036 . . 2035 2038 + HaeIII GGCC 2036 2036 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 74 280 419 440 833 # # AcyI: # [GRCGYC] # 191 1861 # # AflIII: # [ACRYGT] # 840 1212 # # AhaIII*: # [TTTAAA] # 31 2021 # # AjuI: # [GAANNNNNNNTTGG] # 32 508 1512 # # AloI: # [GAACNNNNNNTCC] # 32 32 302 338 1348 # # AluI: # [AGCT] # 320 667 1065 # # AlwNI: # [CAGNNNCTG] # 33 1007 1012 # # ApaI: # [GGGCCC] # 569 1483 # # ApoI: # [RAATTY] # 81 906 1065 # # AsuI*: # [GGNCC] # 1 18 264 554 1215 # # AsuII: # [TTCGAA] # 115 1937 # # AvaI: # [CYCGRG] # 951 1101 # # AvaII: # [GGWCC] # 264 1788 # # BamHI: # [GGATCC] # 352 1700 # # BbvCI: # [CCTCAGC] # 328 1724 # # BbvI: # [GCAGC] # 9 420 434 1189 # # BccI: # [CCATC] # 113 253 327 632 727 # # Bce83I*: # [CTTGAG] # 311 1741 # # BceAI: # [ACGGC] # 71 133 176 767 905 # # BcgI: # [CGANNNNNNTGC] # 34 987 1031 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 628 1390 # # BinI*: # [GGATC] # 13 40 134 137 140 205 # 347 1036 # # BisI: # [GCNGC] # 3 9 52 354 434 1200 # # BlsI: # [GCNGC] # 3 9 52 354 435 1199 # # BmeT110I: # [CYCGRG] # 950 1102 # # BmgT120I: # [GGNCC] # 1 17 265 554 1215 # # BplI: # [GAGNNNNNCTC] # 0 0 32 835 1185 # # Bpu10I: # [CCTNAGC] # 328 1724 # # BsaBI: # [GATNNNNATC] # 646 1406 # # BseBI: # [CCWGG] # 632 684 736 # # BseGI: # [GGATG] # 338 528 582 604 # # BseMII: # [CTCAG] # 75 312 314 565 786 # # BseRI: # [GAGGAG] # 539 1513 # # BseSI: # [GKGCMC] # 388 569 1095 # # BsiYI*: # [CCNNNNNNNGG] # 1 245 282 302 423 799 # # BsmAI: # [GTCTC] # 41 150 495 1366 # # Bsp120I: # [GGGCCC] # 573 1479 # # BspCNI: # [CTCAG] # 77 310 315 564 786 # # BspHI: # [TCATGA] # 218 279 1555 # # BspMI: # [ACCTGC] # 17 368 423 1244 # # BsrDI: # [GCAATG] # 290 571 1191 # # BsrI: # [ACTGG] # 122 251 1679 # # BssKI: # [CCNGG] # 630 686 736 # # BstAPI: # [GCANNNNNTGC] # 215 430 1407 # # BstKTI: # [GATC] # 27 132 147 150 192 355 # 1049 # # BtrI: # [CACGTC] # 680 1372 # # BtsI: # [GCAGTG] # 563 1489 # # Cac8I: # [GCNNGC] # 27 48 322 815 840 # # CfrI: # [YGGCCR] # 797 1255 # # Csp6I: # [GTAC] # 44 53 129 151 166 202 # 287 417 603 # # CspCI: # [CAANNNNNGTGG] # 35 784 1233 # # CviAII: # [CATG] # 18 20 29 70 84 94 # 111 128 149 155 167 183 # 190 264 390 # # CviJI: # [RGCY] # 11 16 21 26 37 45 # 56 76 81 94 108 132 # 164 168 195 197 261 364 # # CviRI*: # [TGCA] # 19 23 26 29 31 36 # 73 82 92 93 100 147 # 159 345 797 # # DdeI: # [CTNAG] # 54 98 289 328 497 786 # # DpnI: # [GATC] # 27 133 147 150 192 354 # 1049 # # DraII: # [RGGNCCY] # 572 1480 # # DraIII: # [CACNNNGTG] # 1025 1027 # # DsaI*: # [CCRYGG] # 736 1316 # # EciI: # [GGCGGA] # 509 1543 # # Eco31I: # [GGTCTC] # 495 1557 # # Eco57I: # [CTGAAG] # 883 1169 # # Eco57MI: # [CTGRAG] # 390 779 883 # # EcoP15I: # [CAGCAG] # 291 694 1067 # # EcoRI: # [GAATTC] # 987 1065 # # EcoRII: # [CCWGG] # 630 686 736 # # EcoRV: # [GATATC] # 973 1079 # # EcoT22I: # [ATGCAT] # 75 815 1162 # # Esp3I: # [CGTCTC] # 686 1366 # # FatI: # [CATG] # 18 20 29 69 84 94 # 111 128 149 155 168 183 # 190 264 390 # # FokI: # [GGATG] # 345 548 575 584 # # GsuI: # [CTGGAG] # 390 1662 # # HaeIII: # [GGCC] # 16 555 682 799 # # HgaI: # [GACGC] # 180 857 1015 # # HgiCI*: # [GGYRCC] # 962 1090 # # HgiJII*: # [GRGCYC] # 95 474 1483 # # Hin4I: # [GAYNNNNNVTC] # 32 904 1116 # # Hin4II*: # [CCTTC] # 140 304 1608 # # HindIII: # [AAGCTT] # 322 1730 # # HinfI: # [GANTC] # 9 45 48 52 112 119 # 153 173 410 430 501 # # HphI: # [GGTGA] # 83 98 196 392 539 744 # # Hpy166II: # [GTNNAC] # 233 275 489 1055 # # Hpy178III*: # [TCNNGA] # 131 147 177 183 206 219 # 364 625 # # Hpy188I: # [TCNGA] # 156 181 240 348 398 729 # # Hpy99I: # [CGWCG] # 57 996 999 # # Ksp632I*: # [CTCTTC] # 771 1281 # # MaeI: # [CTAG] # 41 151 331 419 542 568 # # MaeII: # [ACGT] # 149 535 679 689 # # MaeIII: # [GTNAC] # 26 246 299 402 513 566 # # MboI: # [GATC] # 27 135 147 150 192 352 # 1049 # # MboII: # [GAAGA] # 18 85 131 152 487 521 # 658 # # MmeI: # [TCCRAC] # 509 602 941 # # MnlI: # [CCTC] # 10 21 34 37 69 74 # 108 128 148 149 214 282 # 331 447 # # MseI: # [TTAA] # 32 45 49 60 87 98 # 113 191 227 308 340 502 # # MslI: # [CAYNNNNRTG] # 910 1142 # # MwoI: # [GCNNNNNNNGC] # 3 9 9 64 80 114 # 366 1407 # # NcoI: # [CCATGG] # 736 1316 # # NlaIII: # [CATG] # 18 20 29 73 84 94 # 111 128 149 155 164 183 # 190 264 390 # # NlaIV: # [GGNNCC] # 17 196 354 358 389 738 # # NspBII*: # [CMGCKG] # 442 1610 # # NspI: # [RCATGY] # 73 819 1160 # # PflMI: # [CCANNNNNTGG] # 245 1807 # # PfoI: # [TCCNGGA] # 686 1366 # # PsiI: # [TTATAA] # 226 1826 # # PsrI: # [GAACNNNNNNTAC] # 32 720 1300 # # PstI: # [CTGCAG] # 788 1264 # # RsaI: # [GTAC] # 45 53 129 150 166 202 # 287 417 603 # # ScaI: # [AGTACT] # 45 2007 # # ScrFI: # [CCNGG] # 632 684 736 # # SduI: # [GDGCHC] # 95 388 474 1095 # # SecI*: # [CCNNGG] # 736 1316 # # SetI: # [ASST] # 13 19 33 37 52 62 # 62 84 87 102 124 194 # 204 219 343 417 # # SexAI: # [ACCWGGT] # 630 1422 # # SfaNI: # [GCATC] # 82 198 343 345 524 560 # # SfeI*: # [CTRYAG] # 529 739 784 # # SmlI: # [CTYRAG] # 326 1726 # # SpeI: # [ACTAGT] # 40 693 1319 # # SphI: # [GCATGC] # 892 1160 # # SspI: # [AATATT] # 99 102 394 698 759 # # StyI: # [CCWWGG] # 736 1316 # # TaiI: # [ACGT] # 149 535 682 686 # # TaqI: # [TCGA] # 115 125 172 338 432 870 # # TaqII: # [CACCCA] # 325 1727 # # TatI: # [WGTACW] # 43 202 1807 # # TauI: # [GCSGC] # 354 448 1250 # # TfiI: # [GAWTC] # 9 45 48 52 112 119 # 153 173 410 430 501 # # TseI: # [GCWGC] # 9 409 433 1201 # # TsoI: # [TARCCA] # 25 338 399 548 742 # # Tsp45I: # [GTSAC] # 701 1351 # # Tsp4CI*: # [ACNGT] # 35 39 60 78 263 430 # 547 600 # # TspDTI: # [ATGAA] # 2 6 24 63 100 129 # 150 151 201 211 229 786 # # TspEI: # [AATT] # 35 52 69 81 97 113 # 169 173 210 216 236 601 # # TspGWI: # [ACGGA] # 175 318 329 1230 # # TspRI: # [CASTGNN] # 570 1482 # # TstI: # [CACNNNNNNTCC] # 32 324 1696 # # VspI: # [ATTAAT] # 878 1174 # # XbaI: # [TCTAGA] # 582 1470 # # XhoII: # [RGATCY] # 135 352 1565 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2052 # Reported_sequences: 1 # Reported_hitcount: 467 #---------------------------------------