Restriction Fragments for OPI9/YLR338W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

OPI9/YLR338W on chromosome XII from coordinates 804346 to 805203.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.6454.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 14 + PleI GAGTC 18 19 . . 10 14 + MlyI GAGTC 19 19 . . 10 14 + HinfI GANTC 10 13 . . 14 18 + BssKI CCNGG 13 18 . . 14 18 + BseBI CCWGG 15 16 . . 14 18 + ScrFI CCNGG 15 16 . . 14 18 + EcoRII CCWGG 13 18 . . 17 20 + CviJI RGCY 18 18 . . 17 20 + HaeIII GGCC 18 18 . . 19 29 + BsiYI* CCNNNNNNNGG 25 22 . . 19 29 + PflMI CCANNNNNTGG 25 22 . . 20 25 + MfeI CAATTG 20 24 . . 21 24 + TspEI AATT 20 24 . . 25 30 - MmeI TCCRAC 6 4 . . 38 41 - MnlI CCTC 31 30 . . 48 51 + SetI ASST 51 47 . . 48 51 + CviJI RGCY 49 49 . . 48 51 + AluI AGCT 49 49 . . 56 59 - MnlI CCTC 49 48 . . 67 72 + BspLU11I* ACATGT 67 71 . . 67 72 + NspI RCATGY 71 67 . . 67 72 + AflIII ACRYGT 67 71 . . 68 71 + FatI CATG 67 71 . . 68 71 + NlaIII CATG 71 67 . . 68 71 + CviAII CATG 68 70 . . 82 86 + HgaI GACGC 91 96 . . 80 84 - BsmAI GTCTC 74 78 . . 80 85 - Esp3I CGTCTC 74 78 . . 86 91 + Eco57MI CTGRAG 107 105 . . 86 91 + Eco57I CTGAAG 107 105 . . 88 92 - Hin4II* CCTTC 82 81 . . 112 115 + MaeI CTAG 112 114 . . 116 120 + MboII GAAGA 128 127 . . 116 121 - Ksp632I* CTCTTC 111 114 . . 123 128 + SspI AATATT 125 125 . . 131 134 + SetI ASST 134 130 . . 131 134 + CviJI RGCY 132 132 . . 131 134 + AluI AGCT 132 132 . . 146 149 + CviJI RGCY 147 147 . . 157 162 + Cac8I GCNNGC 159 159 . . 160 163 + CviJI RGCY 161 161 . . 160 163 + HaeIII GGCC 161 161 . . 167 172 + Eco57MI CTGRAG 188 186 . . 167 172 + Eco57I CTGAAG 188 186 . . 169 173 - Hin4II* CCTTC 163 162 . . 174 179 + NdeI CATATG 175 177 . . 179 182 - AciI CCGC 179 181 . . 193 198 + KpnI GGTACC 197 193 . . 193 198 + NlaIV GGNNCC 195 195 . . 193 198 + HgiCI* GGYRCC 193 197 . . 193 198 + Acc65I GGTACC 193 197 . . 194 197 + Csp6I GTAC 194 196 . . 194 197 + RsaI GTAC 195 195 . . 191 194 - AciI CCGC 191 193 . . 196 199 + SetI ASST 199 195 . . 210 215 + NlaIV GGNNCC 212 212 . . 210 215 + HgiCI* GGYRCC 210 214 . . 208 211 - MnlI CCTC 201 200 . . 213 216 + SetI ASST 216 212 . . 214 224 + BsiYI* CCNNNNNNNGG 220 217 . . 227 230 - AciI CCGC 227 229 . . 236 241 + Eco57MI CTGRAG 257 255 . . 236 241 + GsuI CTGGAG 257 255 . . 248 251 + Csp6I GTAC 248 250 . . 244 248 - BccI CCATC 238 239 . . 248 251 + RsaI GTAC 249 249 . . 265 270 + NlaIV GGNNCC 267 267 . . 263 266 - MnlI CCTC 256 255 . . 266 270 + BmgT120I GGNCC 267 268 . . 266 270 + AsuI* GGNCC 266 269 . . 267 270 + CviJI RGCY 268 268 . . 267 270 + HaeIII GGCC 268 268 . . 268 272 + TauI GCSGC 271 268 . . 268 272 + BlsI GCNGC 270 269 . . 268 272 + BisI GCNGC 269 270 . . 269 272 + AciI CCGC 269 271 . . 274 278 + MboII GAAGA 286 285 . . 277 287 + Hin4I GAYNNNNNVTC 268 263 300 295 277 281 - BccI CCATC 271 272 . . 286 291 + Hpy178III* TCNNGA 287 289 . . 277 287 - Hin4I GAYNNNNNVTC 300 295 268 263 291 294 + TspEI AATT 290 294 . . 297 300 + SetI ASST 300 296 . . 296 299 - MnlI CCTC 289 288 . . 299 304 + BsgI GTGCAG 320 318 . . 300 303 + CviRI* TGCA 301 301 . . 304 308 + MboII GAAGA 316 315 . . 304 309 + BbvII* GAAGAC 311 315 . . 310 313 - MnlI CCTC 303 302 . . 319 322 + MaeI CTAG 319 321 . . 326 331 + NspBII* CMGCKG 328 328 . . 328 331 - AciI CCGC 328 330 . . 328 332 - FauI CCCGC 321 323 . . 337 341 + HphI GGTGA 349 348 . . 348 351 + CviRI* TGCA 349 349 . . 364 369 + NlaIV GGNNCC 366 366 . . 366 369 + CviJI RGCY 367 367 . . 367 371 + TauI GCSGC 370 367 . . 367 371 + BlsI GCNGC 369 368 . . 367 371 + BisI GCNGC 368 369 . . 368 371 + AciI CCGC 368 370 . . 379 384 + NlaIV GGNNCC 381 381 . . 379 384 + HgiCI* GGYRCC 379 383 . . 376 380 - BccI CCATC 370 371 . . 383 386 + AciI CCGC 383 385 . . 384 387 + FnuDII* CGCG 385 385 . . 385 388 + Hin6I GCGC 385 387 . . 385 388 + GlaI GCGC 386 386 . . 385 388 + HhaI GCGC 387 385 . . 388 392 + BssKI CCNGG 387 392 . . 388 392 + ScrFI CCNGG 389 390 . . 388 392 + CauII* CCSGG 389 390 . . 388 398 + BsiYI* CCNNNNNNNGG 394 391 . . 389 392 + HpaII CCGG 389 391 . . 393 396 + TspEI AATT 392 396 . . 398 401 - AciI CCGC 398 400 . . 403 406 + Hin6I GCGC 403 405 . . 403 406 + GlaI GCGC 404 404 . . 403 406 + HhaI GCGC 405 403 . . 405 410 + Cac8I GCNNGC 407 407 . . 412 416 - BccI CCATC 406 407 . . 418 421 - MnlI CCTC 411 410 . . 429 434 + Cac8I GCNNGC 431 431 . . 432 435 + CviRI* TGCA 433 433 . . 452 455 + MseI TTAA 452 454 . . 461 466 + Hpy166II GTNNAC 463 463 . . 461 466 + BssNAI GTATAC 463 463 . . 461 466 + AccI GTMKAC 462 464 . . 465 468 + SetI ASST 468 464 . . 466 469 + MnlI CCTC 476 475 . . 471 474 + SetI ASST 474 470 . . 471 474 + CviJI RGCY 472 472 . . 471 474 + AluI AGCT 472 472 . . 467 472 - Eco57MI CTGRAG 452 450 . . 467 472 - GsuI CTGGAG 452 450 . . 484 494 + BsiYI* CCNNNNNNNGG 490 487 . . 491 494 - AciI CCGC 491 493 . . 495 500 + HaeII RGCGCY 499 495 . . 496 499 + Hin6I GCGC 496 498 . . 496 499 + GlaI GCGC 497 497 . . 496 499 + HhaI GCGC 498 496 . . 499 509 + BsiYI* CCNNNNNNNGG 505 502 . . 500 504 + BccI CCATC 508 509 . . 500 510 + BsiYI* CCNNNNNNNGG 506 503 . . 504 510 + PasI CCCWGGG 505 508 . . 494 504 - Hin4I GAYNNNNNVTC 517 512 485 480 504 509 + SecI* CCNNGG 504 508 . . 501 505 - FokI GGATG 487 491 . . 505 509 + BssKI CCNGG 504 509 . . 501 505 - BseGI GGATG 500 498 . . 505 509 + BseBI CCWGG 506 507 . . 505 510 + SecI* CCNNGG 505 509 . . 505 509 + ScrFI CCNGG 506 507 . . 505 509 + EcoRII CCWGG 504 509 . . 505 515 + BsiYI* CCNNNNNNNGG 511 508 . . 505 515 + PflMI CCANNNNNTGG 511 508 . . 515 520 + BseRI GAGGAG 530 528 . . 515 518 - MnlI CCTC 508 507 . . 519 522 + SetI ASST 522 518 . . 519 522 + CviJI RGCY 520 520 . . 519 522 + AluI AGCT 520 520 . . 531 537 + PpuMI RGGWCCY 532 535 . . 531 537 + DraII RGGNCCY 532 535 . . 531 536 + NlaIV GGNNCC 533 533 . . 529 532 - MnlI CCTC 522 521 . . 532 536 + BmgT120I GGNCC 533 534 . . 532 536 + AvaII GGWCC 532 535 . . 532 536 + AsuI* GGNCC 532 535 . . 547 551 + Tsp4CI* ACNGT 549 548 . . 549 554 + KpnI GGTACC 553 549 . . 549 554 + NlaIV GGNNCC 551 551 . . 549 554 + HgiCI* GGYRCC 549 553 . . 549 554 + Acc65I GGTACC 549 553 . . 550 553 + Csp6I GTAC 550 552 . . 550 553 + RsaI GTAC 551 551 . . 561 566 + Hpy178III* TCNNGA 562 564 . . 550 562 - BarI GAAGNNNNNNTAC 574 569 542 537 562 568 + SauI* CCTNAGG 563 566 . . 563 567 + DdeI CTNAG 563 566 . . 567 570 + CviJI RGCY 568 568 . . 563 567 - BspCNI CTCAG 555 553 . . 567 570 + HaeIII GGCC 568 568 . . 563 567 - BseMII CTCAG 554 552 . . 565 568 - MnlI CCTC 558 557 . . 572 576 + TspDTI ATGAA 587 585 . . 579 589 + Hin4I GAYNNNNNVTC 570 565 602 597 580 590 + BsaXI ACNNNNNCTCC 570 567 600 597 585 588 + SetI ASST 588 584 . . 586 589 + MnlI CCTC 596 595 . . 589 599 + BsiYI* CCNNNNNNNGG 595 592 . . 590 600 + BsiYI* CCNNNNNNNGG 596 593 . . 599 604 + SduI GDGCHC 603 599 . . 599 604 + BseSI GKGCMC 603 599 . . 615 620 + Eco31I GGTCTC 621 625 . . 616 620 + BsmAI GTCTC 621 625 . . 618 622 + DdeI CTNAG 618 621 . . 618 622 + BspCNI CTCAG 631 629 . . 618 622 + BseMII CTCAG 632 630 . . 621 624 + SetI ASST 624 620 . . 621 624 + CviJI RGCY 622 622 . . 621 624 + AluI AGCT 622 622 . . 634 638 - TspDTI ATGAA 624 622 . . 651 655 + Tsp45I GTSAC 650 655 . . 651 655 + MaeIII GTNAC 650 655 . . 654 657 + SetI ASST 657 653 . . 652 656 - HphI GGTGA 644 643 . . 663 668 + AcyI GRCGYC 664 666 . . 666 670 + Tsp45I GTSAC 665 670 . . 666 670 + MaeIII GTNAC 665 670 . . 664 668 - HgaI GACGC 653 658 . . 669 673 + BceAI ACGGC 685 687 . . 671 674 + CviJI RGCY 672 672 . . 671 674 + HaeIII GGCC 672 672 . . 671 675 + BmgT120I GGNCC 672 673 . . 671 675 + AsuI* GGNCC 671 674 . . 676 679 + CviRI* TGCA 677 677 . . 677 681 + SfaNI GCATC 686 690 . . 691 696 + Cfr10I RCCGGY 691 695 . . 692 695 + HpaII CCGG 692 694 . . 701 706 + SduI GDGCHC 705 701 . . 701 706 + HgiAI* GWGCWC 705 701 . . 711 714 + SetI ASST 714 710 . . 712 722 + BsiYI* CCNNNNNNNGG 718 715 . . 725 728 - MnlI CCTC 718 717 . . 728 731 - AciI CCGC 728 730 . . 734 739 + BseRI GAGGAG 749 747 . . 734 737 - MnlI CCTC 727 726 . . 737 742 + BseRI GAGGAG 752 750 . . 737 740 - MnlI CCTC 730 729 . . 740 745 + BseRI GAGGAG 755 753 . . 740 743 - MnlI CCTC 733 732 . . 744 747 + SetI ASST 747 743 . . 744 747 + CviJI RGCY 745 745 . . 744 747 + AluI AGCT 745 745 . . 746 751 + Eco57MI CTGRAG 767 765 . . 746 751 + GsuI CTGGAG 767 765 . . 749 754 + SduI GDGCHC 753 749 . . 749 754 + HgiAI* GWGCWC 753 749 . . 752 758 + AarI CACCTGC 762 766 . . 753 756 + SetI ASST 756 752 . . 753 758 + BspMI ACCTGC 762 766 . . 768 771 + TspEI AATT 767 771 . . 788 791 + CviJI RGCY 789 789 . . 792 796 - MboII GAAGA 784 783 . . 802 806 + Tsp4CI* ACNGT 804 803 . . 810 813 + Hin6I GCGC 810 812 . . 810 813 + GlaI GCGC 811 811 . . 810 813 + HhaI GCGC 812 810 . . 832 836 + BsmAI GTCTC 837 841 . . 850 854 - MboII GAAGA 842 841 . . 852 856 - TspDTI ATGAA 842 840 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 96 762 # # Acc65I: # [GGTACC] # 193 309 356 # # AccI: # [GTMKAC] # 396 462 # # AciI: # [CCGC] # 12 15 15 36 40 42 # 59 93 130 179 237 # # AcyI: # [GRCGYC] # 194 664 # # AflIII: # [ACRYGT] # 67 791 # # AluI: # [AGCT] # 48 49 83 102 113 123 # 340 # # AsuI*: # [GGNCC] # 139 187 266 266 # # AvaII: # [GGWCC] # 326 532 # # BarI: # [GAAGNNNNNNTAC] # 32 284 542 # # BbvII*: # [GAAGAC] # 311 547 # # BccI: # [CCATC] # 33 36 99 102 238 350 # # BceAI: # [ACGGC] # 173 685 # # BisI: # [GCNGC] # 99 269 490 # # BlsI: # [GCNGC] # 99 270 489 # # BmgT120I: # [GGNCC] # 139 186 266 267 # # BsaXI: # [ACNNNNNCTCC] # 30 258 570 # # BseBI: # [CCWGG] # 15 352 491 # # BseGI: # [GGATG] # 358 500 # # BseMII: # [CTCAG] # 78 226 554 # # BseRI: # [GAGGAG] # 3 3 103 219 530 # # BseSI: # [GKGCMC] # 255 603 # # BsgI: # [GTGCAG] # 320 538 # # BsiYI*: # [CCNNNNNNNGG] # 1 1 5 15 25 84 # 96 122 140 174 195 # # BsmAI: # [GTCTC] # 21 74 216 547 # # BspCNI: # [CTCAG] # 76 227 555 # # BspLU11I*: # [ACATGT] # 67 791 # # BspMI: # [ACCTGC] # 96 762 # # BssKI: # [CCNGG] # 13 117 354 374 # # BssNAI: # [GTATAC] # 395 463 # # Cac8I: # [GCNNGC] # 24 159 248 427 # # CauII*: # [CCSGG] # 389 469 # # Cfr10I: # [RCCGGY] # 167 691 # # Csp6I: # [GTAC] # 54 194 302 308 # # CviAII: # [CATG] # 68 790 # # CviJI: # [RGCY] # 14 15 18 31 44 48 # 48 50 54 69 73 83 # 99 105 107 # # CviRI*: # [TGCA] # 48 84 181 244 301 # # DdeI: # [CTNAG] # 55 240 563 # # DraII: # [RGGNCCY] # 326 532 # # Eco31I: # [GGTCTC] # 237 621 # # Eco57I: # [CTGAAG] # 81 107 670 # # Eco57MI: # [CTGRAG] # 69 81 91 107 195 315 # # EcoRII: # [CCWGG] # 13 354 491 # # Esp3I: # [CGTCTC] # 74 784 # # FatI: # [CATG] # 67 791 # # FauI: # [CCCGC] # 321 537 # # FnuDII*: # [CGCG] # 385 473 # # FokI: # [GGATG] # 371 487 # # GlaI: # [GCGC] # 18 47 93 314 386 # # GsuI: # [CTGGAG] # 91 195 257 315 # # HaeII: # [RGCGCY] # 359 499 # # HaeIII: # [GGCC] # 18 104 107 143 186 300 # # HgaI: # [GACGC] # 91 205 562 # # HgiAI*: # [GWGCWC] # 48 105 705 # # HgiCI*: # [GGYRCC] # 17 169 170 193 309 # # HhaI: # [GCGC] # 18 46 93 314 387 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 53 # 185 256 268 # # Hin4II*: # [CCTTC] # 81 82 695 # # Hin6I: # [GCGC] # 18 48 93 314 385 # # HinfI: # [GANTC] # 10 848 # # HpaII: # [CCGG] # 166 303 389 # # HphI: # [GGTGA] # 214 295 349 # # Hpy166II: # [GTNNAC] # 395 463 # # Hpy178III*: # [TCNNGA] # 275 287 296 # # KpnI: # [GGTACC] # 197 305 356 # # Ksp632I*: # [CTCTTC] # 111 747 # # MaeI: # [CTAG] # 112 207 539 # # MaeIII: # [GTNAC] # 15 193 650 # # MboII: # [GAAGA] # 16 30 58 128 158 468 # # MfeI: # [CAATTG] # 20 838 # # MlyI: # [GAGTC] # 19 839 # # MmeI: # [TCCRAC] # 6 852 # # MnlI: # [CCTC] # 3 3 9 14 14 18 # 31 32 33 36 38 55 # 65 108 122 125 152 # # MseI: # [TTAA] # 406 452 # # NdeI: # [CATATG] # 175 683 # # NlaIII: # [CATG] # 71 787 # # NlaIV: # [GGNNCC] # 15 17 18 55 99 152 # 195 307 # # NspBII*: # [CMGCKG] # 328 530 # # NspI: # [RCATGY] # 71 787 # # PasI: # [CCCWGGG] # 353 505 # # PflMI: # [CCANNNNNTGG] # 25 347 486 # # PleI: # [GAGTC] # 18 840 # # PpuMI: # [RGGWCCY] # 326 532 # # RsaI: # [GTAC] # 54 195 302 307 # # SauI*: # [CCTNAGG] # 295 563 # # ScrFI: # [CCNGG] # 15 117 352 374 # # SduI: # [GDGCHC] # 48 102 105 603 # # SecI*: # [CCNNGG] # 1 353 504 # # SetI: # [ASST] # 6 9 17 33 33 36 # 48 51 57 65 66 83 # 84 102 168 # # SfaNI: # [GCATC] # 172 686 # # SspI: # [AATATT] # 125 733 # # TauI: # [GCSGC] # 99 271 488 # # Tsp45I: # [GTSAC] # 15 193 650 # # Tsp4CI*: # [ACNGT] # 54 255 549 # # TspDTI: # [ATGAA] # 16 37 218 587 # # TspEI: # [AATT] # 20 91 102 270 375 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 858 # Reported_sequences: 1 # Reported_hitcount: 251 #---------------------------------------