Restriction Fragments for RPS25B/YLR333C



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The currently selected gene/sequence is :

RPS25B/YLR333C on chromosome XII from coordinates 795899 to 795573.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22770.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 13 + MwoI GCNNNNNNNGC 9 6 . . 4 7 + MnlI CCTC 14 13 . . 16 21 + MfeI CAATTG 16 20 . . 17 20 + TspEI AATT 16 20 . . 27 30 + SetI ASST 30 26 . . 27 30 + CviJI RGCY 28 28 . . 27 30 + AluI AGCT 28 28 . . 28 38 + MwoI GCNNNNNNNGC 34 31 . . 28 32 + BlsI GCNGC 30 29 . . 28 32 + BisI GCNGC 29 30 . . 28 32 + TseI GCWGC 28 31 . . 31 41 + MwoI GCNNNNNNNGC 37 34 . . 28 32 - BbvI GCAGC 15 19 . . 36 39 + CviJI RGCY 37 37 . . 37 41 + TauI GCSGC 40 37 . . 37 47 + MwoI GCNNNNNNNGC 43 40 . . 37 41 + BlsI GCNGC 39 38 . . 37 41 + BisI GCNGC 38 39 . . 38 43 + NspBII* CMGCKG 40 40 . . 38 41 + AciI CCGC 38 40 . . 40 44 + BlsI GCNGC 42 41 . . 40 44 + BisI GCNGC 41 42 . . 40 44 + TseI GCWGC 40 43 . . 43 47 + TauI GCSGC 46 43 . . 43 53 + MwoI GCNNNNNNNGC 49 46 . . 43 47 + BlsI GCNGC 45 44 . . 43 47 + BisI GCNGC 44 45 . . 40 44 - BbvI GCAGC 27 31 . . 44 47 + AciI CCGC 44 46 . . 44 49 + BsrBI CCGCTC 46 46 . . 72 76 + MboII GAAGA 84 83 . . 80 84 + BmgT120I GGNCC 81 82 . . 80 84 + AvaII GGWCC 80 83 . . 80 84 + AsuI* GGNCC 80 83 . . 93 96 + FatI CATG 92 96 . . 93 96 + NlaIII CATG 96 92 . . 93 96 + CviAII CATG 93 95 . . 94 98 + TspDTI ATGAA 109 107 . . 96 100 - Hin4II* CCTTC 90 89 . . 104 109 + SacI GAGCTC 108 104 . . 104 109 + Ecl136II GAGCTC 106 106 . . 104 109 + SduI GDGCHC 108 104 . . 104 109 + HgiJII* GRGCYC 108 104 . . 104 109 + HgiAI* GWGCWC 108 104 . . 105 108 + SetI ASST 108 104 . . 105 108 + CviJI RGCY 106 106 . . 105 108 + AluI AGCT 106 106 . . 106 116 + MwoI GCNNNNNNNGC 112 109 . . 126 130 + BinI* GGATC 134 135 . . 127 130 + DpnI GATC 128 128 . . 127 130 + BstKTI GATC 129 127 . . 127 130 + MboI GATC 126 130 . . 129 134 + Hpy178III* TCNNGA 130 132 . . 146 150 + TfiI GAWTC 146 149 . . 146 150 + HinfI GANTC 146 149 . . 149 154 + Hpy178III* TCNNGA 150 152 . . 153 157 - Hin4II* CCTTC 147 146 . . 158 161 + SetI ASST 161 157 . . 159 164 + NlaIV GGNNCC 161 161 . . 157 160 - MnlI CCTC 150 149 . . 162 167 + MmeI TCCRAC 187 185 . . 176 179 + TaiI ACGT 179 175 . . 176 179 + SetI ASST 179 175 . . 176 179 + MaeII ACGT 176 178 . . 177 182 + Esp3I CGTCTC 183 187 . . 178 182 + BsmAI GTCTC 183 187 . . 187 191 - TspGWI ACGGA 177 175 . . 205 208 + MseI TTAA 205 207 . . 226 236 + MwoI GCNNNNNNNGC 232 229 . . 231 234 + TspEI AATT 230 234 . . 240 243 - MnlI CCTC 233 232 . . 258 261 + SetI ASST 261 257 . . 256 260 - Hin4II* CCTTC 250 249 . . 269 272 + CviJI RGCY 270 270 . . 275 280 + Hpy178III* TCNNGA 276 278 . . 277 280 + TaqI TCGA 277 279 . . 282 292 + MwoI GCNNNNNNNGC 288 285 . . 282 287 - BsmI GAATGC 282 280 . . 291 296 + Cac8I GCNNGC 293 293 . . 294 297 + SetI ASST 297 293 . . 294 297 + CviJI RGCY 295 295 . . 294 297 + AluI AGCT 295 295 . . 309 312 + SetI ASST 312 308 . . 309 312 + CviJI RGCY 310 310 . . 309 312 + AluI AGCT 310 310 . . 310 314 + BlsI GCNGC 312 311 . . 310 314 + BisI GCNGC 311 312 . . 310 314 + TseI GCWGC 310 313 . . 313 317 + BlsI GCNGC 315 314 . . 313 317 + BisI GCNGC 314 315 . . 313 317 + TseI GCWGC 313 316 . . 310 314 - BbvI GCAGC 297 301 . . 311 316 - EcoP15I CAGCAG 283 285 . . 313 317 - BbvI GCAGC 300 304 . . 319 323 + Hpy188I TCNGA 321 320 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 38 283 # # AluI: # [AGCT] # 15 17 28 78 189 # # AsuI*: # [GGNCC] # 80 247 # # AvaII: # [GGWCC] # 80 247 # # BbvI: # [GCAGC] # 3 12 15 27 270 # # BinI*: # [GGATC] # 134 193 # # BisI: # [GCNGC] # 3 3 3 9 13 29 # 267 # # BlsI: # [GCNGC] # 3 3 3 9 12 30 # 267 # # BmgT120I: # [GGNCC] # 81 246 # # BsmAI: # [GTCTC] # 144 183 # # BsmI: # [GAATGC] # 45 282 # # BsrBI: # [CCGCTC] # 46 281 # # BstKTI: # [GATC] # 129 198 # # Cac8I: # [GCNNGC] # 34 293 # # CviAII: # [CATG] # 93 234 # # CviJI: # [RGCY] # 9 15 17 25 28 69 # 164 # # DpnI: # [GATC] # 128 199 # # Ecl136II: # [GAGCTC] # 106 221 # # EcoP15I: # [CAGCAG] # 44 283 # # Esp3I: # [CGTCTC] # 144 183 # # FatI: # [CATG] # 92 235 # # HgiAI*: # [GWGCWC] # 108 219 # # HgiJII*: # [GRGCYC] # 108 219 # # Hin4II*: # [CCTTC] # 57 77 90 103 # # HinfI: # [GANTC] # 146 181 # # Hpy178III*: # [TCNNGA] # 20 51 126 130 # # Hpy188I: # [TCNGA] # 6 321 # # MaeII: # [ACGT] # 151 176 # # MboI: # [GATC] # 126 201 # # MboII: # [GAAGA] # 84 243 # # MfeI: # [CAATTG] # 16 311 # # MmeI: # [TCCRAC] # 140 187 # # MnlI: # [CCTC] # 14 83 94 136 # # MseI: # [TTAA] # 122 205 # # MwoI: # [GCNNNNNNNGC] # 3 6 6 9 25 39 # 56 63 120 # # NlaIII: # [CATG] # 96 231 # # NlaIV: # [GGNNCC] # 161 166 # # NspBII*: # [CMGCKG] # 40 287 # # SacI: # [GAGCTC] # 108 219 # # SduI: # [GDGCHC] # 108 219 # # SetI: # [ASST] # 15 15 18 30 36 53 # 78 82 # # TaiI: # [ACGT] # 148 179 # # TaqI: # [TCGA] # 50 277 # # TauI: # [GCSGC] # 6 40 281 # # TfiI: # [GAWTC] # 146 181 # # TseI: # [GCWGC] # 3 12 14 28 270 # # TspDTI: # [ATGAA] # 109 218 # # TspEI: # [AATT] # 16 97 214 # # TspGWI: # [ACGGA] # 150 177 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 327 # Reported_sequences: 1 # Reported_hitcount: 95 #---------------------------------------