Restriction Fragments for CDC25/YLR310C



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The currently selected gene/sequence is :

CDC25/YLR310C on chromosome XII from coordinates 756993 to 752224.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24517.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + Hpy188I TCNGA 6 5 . . 14 19 + AflIII ACRYGT 14 18 . . 15 26 + TstI CACNNNNNNTCC 6 1 38 33 15 20 + BtrI CACGTC 17 17 . . 16 19 + TaiI ACGT 19 15 . . 16 19 + SetI ASST 19 15 . . 16 19 + MaeII ACGT 16 18 . . 39 42 + CviRI* TGCA 40 40 . . 40 50 + MwoI GCNNNNNNNGC 46 43 . . 46 49 - MnlI CCTC 39 38 . . 49 59 + MwoI GCNNNNNNNGC 55 52 . . 49 54 + Cac8I GCNNGC 51 51 . . 49 59 + BstAPI GCANNNNNTGC 55 52 . . 53 58 + BsrDI GCAATG 60 58 . . 70 74 + BccI CCATC 78 79 . . 72 77 + ClaI ATCGAT 73 75 . . 73 76 + TaqI TCGA 73 75 . . 75 78 + DpnI GATC 76 76 . . 75 78 + BstKTI GATC 77 75 . . 75 78 + MboI GATC 74 78 . . 79 82 + SetI ASST 82 78 . . 79 82 + CviJI RGCY 80 80 . . 79 82 + AluI AGCT 80 80 . . 80 86 + SapI GCTCTTC 87 90 . . 81 86 + Ksp632I* CTCTTC 87 90 . . 82 86 - MboII GAAGA 74 73 . . 84 88 - TspDTI ATGAA 74 72 . . 115 119 + TfiI GAWTC 115 118 . . 115 119 + HinfI GANTC 115 118 . . 122 125 + MnlI CCTC 132 131 . . 122 128 + Bpu10I CCTNAGC 123 126 . . 122 128 + BbvCI CCTCAGC 123 126 . . 119 123 - FokI GGATG 105 109 . . 123 127 + DdeI CTNAG 123 126 . . 123 127 + BspCNI CTCAG 136 134 . . 123 127 + BseMII CTCAG 137 135 . . 119 123 - BseGI GGATG 118 116 . . 126 129 + SetI ASST 129 125 . . 126 129 + CviJI RGCY 127 127 . . 126 129 + AluI AGCT 127 127 . . 136 140 - MboII GAAGA 128 127 . . 139 143 - MboII GAAGA 131 130 . . 146 149 + MnlI CCTC 156 155 . . 148 151 + TaqI TCGA 148 150 . . 159 164 + Hpy166II GTNNAC 161 161 . . 159 164 + HindII GTYRAC 161 161 . . 167 172 - BsmI GAATGC 167 165 . . 176 181 + MfeI CAATTG 176 180 . . 177 180 + TspEI AATT 176 180 . . 193 197 + BlsI GCNGC 195 194 . . 193 197 + BisI GCNGC 194 195 . . 193 197 + TseI GCWGC 193 196 . . 193 197 - BbvI GCAGC 180 184 . . 206 209 + MseI TTAA 206 208 . . 208 211 + TspEI AATT 207 211 . . 218 221 + MseI TTAA 218 220 . . 227 231 + Tsp4CI* ACNGT 229 228 . . 232 236 - MboII GAAGA 224 223 . . 249 254 + TatI WGTACW 249 253 . . 249 254 + Bsp1407I TGTACA 249 253 . . 250 253 + Csp6I GTAC 250 252 . . 250 253 + RsaI GTAC 251 251 . . 278 281 + MseI TTAA 278 280 . . 290 299 + MslI CAYNNNNRTG 294 294 . . 294 299 - TaqII CACCCA 284 282 . . 303 307 + FokI GGATG 316 320 . . 303 307 + BseGI GGATG 309 307 . . 304 308 - BccI CCATC 298 299 . . 323 327 + Tsp4CI* ACNGT 325 324 . . 337 342 + Hpy166II GTNNAC 339 339 . . 337 342 + HpaI GTTAAC 339 339 . . 337 342 + HindII GTYRAC 339 339 . . 338 341 + MseI TTAA 338 340 . . 342 350 + AlwNI CAGNNNCTG 347 344 . . 346 349 + CviJI RGCY 347 347 . . 344 347 - MnlI CCTC 337 336 . . 355 358 + MnlI CCTC 365 364 . . 368 373 + Hpy166II GTNNAC 370 370 . . 368 373 + AccI GTMKAC 369 371 . . 372 375 + SetI ASST 375 371 . . 376 379 + MseI TTAA 376 378 . . 383 387 + Tsp4CI* ACNGT 385 384 . . 380 384 - BsmAI GTCTC 374 378 . . 391 395 + DdeI CTNAG 391 394 . . 391 395 + BspCNI CTCAG 404 402 . . 391 395 + BseMII CTCAG 405 403 . . 392 396 + Hpy188I TCNGA 394 393 . . 401 405 + Tsp4CI* ACNGT 403 402 . . 408 412 + Hpy188I TCNGA 410 409 . . 406 410 - FokI GGATG 392 396 . . 406 410 - BseGI GGATG 405 403 . . 412 416 + TspDTI ATGAA 427 425 . . 428 432 - BsrI ACTGG 428 426 . . 435 440 + SacI GAGCTC 439 435 . . 435 440 + Ecl136II GAGCTC 437 437 . . 435 440 + SduI GDGCHC 439 435 . . 435 440 + HgiJII* GRGCYC 439 435 . . 435 440 + HgiAI* GWGCWC 439 435 . . 436 439 + SetI ASST 439 435 . . 436 439 + CviJI RGCY 437 437 . . 436 439 + AluI AGCT 437 437 . . 437 447 + MwoI GCNNNNNNNGC 443 440 . . 438 443 + SmlI CTYRAG 438 442 . . 438 443 - Bce83I* CTTGAG 423 421 . . 444 447 + Hin6I GCGC 444 446 . . 444 447 + GlaI GCGC 445 445 . . 444 447 + HhaI GCGC 446 444 . . 446 450 + BlsI GCNGC 448 447 . . 446 450 + BisI GCNGC 447 448 . . 446 450 + BbvI GCAGC 458 462 . . 446 450 + TseI GCWGC 446 449 . . 447 452 + EcoP15I CAGCAG 477 479 . . 449 453 + BlsI GCNGC 451 450 . . 449 453 + BisI GCNGC 450 451 . . 449 453 + BbvI GCAGC 461 465 . . 449 453 + TseI GCWGC 449 452 . . 451 454 + SetI ASST 454 450 . . 451 454 + CviJI RGCY 452 452 . . 451 454 + AluI AGCT 452 452 . . 452 462 + MwoI GCNNNNNNNGC 458 455 . . 454 457 + MseI TTAA 454 456 . . 460 463 + SetI ASST 463 459 . . 460 463 + CviJI RGCY 461 461 . . 460 463 + AluI AGCT 461 461 . . 474 477 + CviRI* TGCA 475 475 . . 483 488 + BspLU11I* ACATGT 483 487 . . 483 488 + NspI RCATGY 487 483 . . 483 488 + AflIII ACRYGT 483 487 . . 484 487 + FatI CATG 483 487 . . 484 487 + NlaIII CATG 487 483 . . 484 487 + CviAII CATG 484 486 . . 491 494 + MaeI CTAG 491 493 . . 498 502 + Hpy188I TCNGA 500 499 . . 513 516 - MnlI CCTC 506 505 . . 512 522 - BsaXI ACNNNNNCTCC 534 531 504 501 513 524 - PpiI GAACNNNNNCTC 536 531 504 499 512 524 - AloI GAACNNNNNNTCC 536 531 504 499 535 539 - HgaI GACGC 524 529 . . 550 560 + MwoI GCNNNNNNNGC 556 553 . . 558 561 + CviRI* TGCA 559 559 . . 572 575 + HpaII CCGG 572 574 . . 573 578 + CfrI YGGCCR 573 577 . . 574 577 + CviJI RGCY 575 575 . . 574 577 + HaeIII GGCC 575 575 . . 586 590 + Tsp4CI* ACNGT 588 587 . . 591 596 + ClaI ATCGAT 592 594 . . 592 595 + TaqI TCGA 592 594 . . 595 599 + TspDTI ATGAA 610 608 . . 601 604 + TspEI AATT 600 604 . . 615 620 + HaeII RGCGCY 619 615 . . 616 619 + Hin6I GCGC 616 618 . . 616 619 + GlaI GCGC 617 617 . . 616 619 + HhaI GCGC 618 616 . . 625 629 - SfaNI GCATC 615 619 . . 619 632 - AjuI GAANNNNNNNTTGG 644 639 612 607 643 646 + MnlI CCTC 653 652 . . 651 656 + AsuII TTCGAA 652 654 . . 652 655 + TaqI TCGA 652 654 . . 649 653 - MboII GAAGA 641 640 . . 654 666 + AloI GAACNNNNNNTCC 646 641 678 673 659 662 + MseI TTAA 659 661 . . 661 664 + TspEI AATT 660 664 . . 671 674 + MaeI CTAG 671 673 . . 680 685 + SecI* CCNNGG 680 684 . . 681 685 + BssKI CCNGG 680 685 . . 681 685 + BseBI CCWGG 682 683 . . 681 685 + ScrFI CCNGG 682 683 . . 681 685 + EcoRII CCWGG 680 685 . . 698 703 + BsgI GTGCAG 719 717 . . 699 702 + CviRI* TGCA 700 700 . . 703 713 + Hin4I GAYNNNNNVTC 694 689 726 721 706 709 + CviJI RGCY 707 707 . . 703 707 - BccI CCATC 697 698 . . 712 716 + Hpy188I TCNGA 714 713 . . 703 713 - Hin4I GAYNNNNNVTC 726 721 694 689 713 717 + DdeI CTNAG 713 716 . . 713 717 - BspCNI CTCAG 705 703 . . 713 717 - BseMII CTCAG 704 702 . . 718 722 + TspDTI ATGAA 733 731 . . 732 735 + TspEI AATT 731 735 . . 736 740 + TspDTI ATGAA 751 749 . . 746 750 + TspDTI ATGAA 761 759 . . 753 758 + ApoI RAATTY 753 757 . . 754 757 + TspEI AATT 753 757 . . 769 773 + HphI GGTGA 781 780 . . 780 783 + SetI ASST 783 779 . . 780 786 + PpuMI RGGWCCY 781 784 . . 780 786 + DraII RGGNCCY 781 784 . . 781 786 + NlaIV GGNNCC 783 783 . . 781 785 + BmgT120I GGNCC 782 783 . . 781 785 + AvaII GGWCC 781 784 . . 781 785 + AsuI* GGNCC 781 784 . . 778 783 - BspMI ACCTGC 769 773 . . 782 786 - BslFI GGGAC 767 771 . . 789 794 + SpeI ACTAGT 789 793 . . 790 793 + MaeI CTAG 790 792 . . 799 803 + MboII GAAGA 811 810 . . 807 810 + TspEI AATT 806 810 . . 809 812 + MseI TTAA 809 811 . . 819 823 + Tsp4CI* ACNGT 821 820 . . 826 830 + MboII GAAGA 838 837 . . 822 827 - MmeI TCCRAC 803 801 . . 826 831 - Ksp632I* CTCTTC 821 824 . . 844 849 + VspI ATTAAT 845 847 . . 845 848 + MseI TTAA 845 847 . . 851 856 + Hpy166II GTNNAC 853 853 . . 851 856 + BssNAI GTATAC 853 853 . . 851 856 + AccI GTMKAC 852 854 . . 855 858 + TaiI ACGT 858 854 . . 855 858 + SetI ASST 858 854 . . 855 858 + MaeII ACGT 855 857 . . 858 863 + AsuII TTCGAA 859 861 . . 859 862 + TaqI TCGA 859 861 . . 864 869 - BsrDI GCAATG 863 861 . . 871 874 + TaqI TCGA 871 873 . . 873 881 + Tth111I GACNNNGTC 876 877 . . 878 883 + NlaIV GGNNCC 880 880 . . 878 882 + BmgT120I GGNCC 879 880 . . 878 882 + AvaII GGWCC 878 881 . . 878 882 + AsuI* GGNCC 878 881 . . 879 883 - BslFI GGGAC 864 868 . . 890 896 + TspRI CASTGNN 896 887 . . 907 911 + Hpy188I TCNGA 909 908 . . 905 909 - FokI GGATG 891 895 . . 905 909 - BseGI GGATG 904 902 . . 922 927 + Hpy166II GTNNAC 924 924 . . 922 927 + BssNAI GTATAC 924 924 . . 922 927 + AccI GTMKAC 923 925 . . 938 941 + SetI ASST 941 937 . . 950 954 + Tsp4CI* ACNGT 952 951 . . 955 959 + Hpy188I TCNGA 957 956 . . 959 962 + TspEI AATT 958 962 . . 981 984 + TspEI AATT 980 984 . . 988 992 + TfiI GAWTC 988 991 . . 988 992 + HinfI GANTC 988 991 . . 991 995 + Hpy188I TCNGA 993 992 . . 1004 1008 + Tsp45I GTSAC 1003 1008 . . 1004 1008 + MaeIII GTNAC 1003 1008 . . 1012 1024 + AloI GAACNNNNNNTCC 1004 999 1036 1031 1018 1023 + ApoI RAATTY 1018 1022 . . 1019 1022 + TspEI AATT 1018 1022 . . 1023 1027 + FauI CCCGC 1031 1033 . . 1024 1027 + AciI CCGC 1024 1026 . . 1032 1037 + BclI TGATCA 1032 1036 . . 1033 1036 + DpnI GATC 1034 1034 . . 1033 1036 + BstKTI GATC 1035 1033 . . 1033 1036 + MboI GATC 1032 1036 . . 1042 1047 + Hpy166II GTNNAC 1044 1044 . . 1042 1047 + HpaI GTTAAC 1044 1044 . . 1042 1047 + HindII GTYRAC 1044 1044 . . 1043 1046 + MseI TTAA 1043 1045 . . 1046 1049 + SetI ASST 1049 1045 . . 1055 1058 + MaeI CTAG 1055 1057 . . 1059 1064 + XhoII RGATCY 1059 1063 . . 1059 1064 + BglII AGATCT 1059 1063 . . 1060 1063 + DpnI GATC 1061 1061 . . 1060 1063 + BstKTI GATC 1062 1060 . . 1060 1063 + MboI GATC 1059 1063 . . 1062 1066 + Hpy188I TCNGA 1064 1063 . . 1063 1067 + DdeI CTNAG 1063 1066 . . 1063 1067 - BspCNI CTCAG 1055 1053 . . 1063 1067 - BseMII CTCAG 1054 1052 . . 1065 1068 - MnlI CCTC 1058 1057 . . 1078 1083 + SspI AATATT 1080 1080 . . 1084 1087 - MnlI CCTC 1077 1076 . . 1088 1092 + Tsp4CI* ACNGT 1090 1089 . . 1100 1103 + DpnI GATC 1101 1101 . . 1100 1103 + BstKTI GATC 1102 1100 . . 1100 1103 + MboI GATC 1099 1103 . . 1102 1105 + TaqI TCGA 1102 1104 . . 1111 1115 + Hpy188I TCNGA 1113 1112 . . 1118 1123 + Hpy166II GTNNAC 1120 1120 . . 1130 1133 + DpnI GATC 1131 1131 . . 1130 1133 + BstKTI GATC 1132 1130 . . 1130 1133 + MboI GATC 1129 1133 . . 1135 1138 + CviJI RGCY 1136 1136 . . 1137 1141 + Hin4II* CCTTC 1147 1146 . . 1137 1147 + BsiYI* CCNNNNNNNGG 1143 1140 . . 1156 1160 + TfiI GAWTC 1156 1159 . . 1156 1160 + HinfI GANTC 1156 1159 . . 1171 1175 - BccI CCATC 1165 1166 . . 1184 1188 + Tsp4CI* ACNGT 1186 1185 . . 1189 1194 + VspI ATTAAT 1190 1192 . . 1190 1193 + MseI TTAA 1190 1192 . . 1228 1233 + EcoRI GAATTC 1228 1232 . . 1228 1233 + ApoI RAATTY 1228 1232 . . 1229 1232 + TspEI AATT 1228 1232 . . 1239 1242 + CviJI RGCY 1240 1240 . . 1237 1240 - MnlI CCTC 1230 1229 . . 1243 1247 + BmgT120I GGNCC 1244 1245 . . 1243 1247 + AvaII GGWCC 1243 1246 . . 1243 1247 + AsuI* GGNCC 1243 1246 . . 1246 1250 + Hin4II* CCTTC 1256 1255 . . 1248 1252 - TspDTI ATGAA 1238 1236 . . 1255 1258 + MseI TTAA 1255 1257 . . 1257 1262 + ApoI RAATTY 1257 1261 . . 1258 1261 + TspEI AATT 1257 1261 . . 1272 1277 + Hpy178III* TCNNGA 1273 1275 . . 1271 1276 - Eco57MI CTGRAG 1256 1254 . . 1271 1276 - GsuI CTGGAG 1256 1254 . . 1279 1282 + MseI TTAA 1279 1281 . . 1288 1293 + SspI AATATT 1290 1290 . . 1314 1324 + FalI AAGNNNNNCTT 1305 1300 1337 1332 1319 1325 + SapI GCTCTTC 1326 1329 . . 1320 1325 + Ksp632I* CTCTTC 1326 1329 . . 1314 1324 - FalI AAGNNNNNCTT 1337 1332 1305 1300 1321 1325 - MboII GAAGA 1313 1312 . . 1312 1325 - AjuI GAANNNNNNNTTGG 1337 1332 1305 1300 1329 1333 + BsrI ACTGG 1334 1332 . . 1331 1336 + BalI TGGCCA 1333 1333 . . 1331 1336 + CfrI YGGCCR 1331 1335 . . 1332 1335 + CviJI RGCY 1333 1333 . . 1332 1335 + HaeIII GGCC 1333 1333 . . 1340 1344 + Tsp45I GTSAC 1339 1344 . . 1340 1344 + MaeIII GTNAC 1339 1344 . . 1360 1365 + Hpy178III* TCNNGA 1361 1363 . . 1373 1376 + TspEI AATT 1372 1376 . . 1377 1382 + Hpy166II GTNNAC 1379 1379 . . 1385 1388 + TspEI AATT 1384 1388 . . 1394 1397 + SetI ASST 1397 1393 . . 1399 1403 + Tsp4CI* ACNGT 1401 1400 . . 1417 1421 + BceAI ACGGC 1433 1435 . . 1419 1422 + CviJI RGCY 1420 1420 . . 1436 1439 + MseI TTAA 1436 1438 . . 1443 1447 + TfiI GAWTC 1443 1446 . . 1443 1447 + HinfI GANTC 1443 1446 . . 1447 1452 + Esp3I CGTCTC 1453 1457 . . 1448 1452 + BsmAI GTCTC 1453 1457 . . 1453 1456 + TspEI AATT 1452 1456 . . 1457 1462 + Hpy178III* TCNNGA 1458 1460 . . 1471 1481 + Hin4I GAYNNNNNVTC 1462 1457 1494 1489 1476 1481 + EcoRV GATATC 1478 1478 . . 1480 1484 + Hpy188I TCNGA 1482 1481 . . 1471 1481 - Hin4I GAYNNNNNVTC 1494 1489 1462 1457 1483 1487 + TfiI GAWTC 1483 1486 . . 1483 1487 + HinfI GANTC 1483 1486 . . 1493 1497 - MboII GAAGA 1485 1484 . . 1492 1497 - BbvII* GAAGAC 1485 1489 . . 1500 1504 + Tsp4CI* ACNGT 1502 1501 . . 1507 1510 + CviJI RGCY 1508 1508 . . 1508 1512 + BlsI GCNGC 1510 1509 . . 1508 1512 + BisI GCNGC 1509 1510 . . 1508 1512 + TseI GCWGC 1508 1511 . . 1509 1514 + PstI CTGCAG 1513 1509 . . 1509 1514 + SfeI* CTRYAG 1509 1513 . . 1510 1513 + CviRI* TGCA 1511 1511 . . 1511 1516 + Cac8I GCNNGC 1513 1513 . . 1511 1522 + AlfI GCANNNNNNTGC 1500 1498 1534 1532 1508 1512 - BbvI GCAGC 1495 1499 . . 1514 1517 + CviJI RGCY 1515 1515 . . 1520 1523 + CviRI* TGCA 1521 1521 . . 1511 1522 - AlfI GCANNNNNNTGC 1534 1532 1500 1498 1525 1528 + FatI CATG 1524 1528 . . 1525 1528 + NlaIII CATG 1528 1524 . . 1525 1528 + CviAII CATG 1525 1527 . . 1526 1530 + TspDTI ATGAA 1541 1539 . . 1530 1533 + TspEI AATT 1529 1533 . . 1532 1535 + MseI TTAA 1532 1534 . . 1536 1539 + CviJI RGCY 1537 1537 . . 1546 1549 + CviRI* TGCA 1547 1547 . . 1547 1551 + BlsI GCNGC 1549 1548 . . 1547 1551 + BisI GCNGC 1548 1549 . . 1547 1551 + BbvI GCAGC 1559 1563 . . 1547 1551 + TseI GCWGC 1547 1550 . . 1559 1562 + MseI TTAA 1559 1561 . . 1563 1567 + MboII GAAGA 1575 1574 . . 1563 1572 + XmnI GAANNNNTTC 1567 1567 . . 1575 1578 + SetI ASST 1578 1574 . . 1584 1589 + Ksp632I* CTCTTC 1590 1593 . . 1585 1589 - MboII GAAGA 1577 1576 . . 1587 1591 - TspDTI ATGAA 1577 1575 . . 1599 1608 + BsaBI GATNNNNATC 1603 1603 . . 1609 1612 + TspEI AATT 1608 1612 . . 1625 1628 + TaqI TCGA 1625 1627 . . 1627 1631 + TfiI GAWTC 1627 1630 . . 1627 1631 + HinfI GANTC 1627 1630 . . 1632 1635 + SetI ASST 1635 1631 . . 1632 1635 + CviJI RGCY 1633 1633 . . 1632 1635 + AluI AGCT 1633 1633 . . 1653 1658 - BciVI GTATCC 1647 1646 . . 1660 1664 + TspDTI ATGAA 1675 1673 . . 1683 1688 + Hpy178III* TCNNGA 1684 1686 . . 1693 1696 + TspEI AATT 1692 1696 . . 1700 1703 + MaeI CTAG 1700 1702 . . 1705 1709 + TspGWI ACGGA 1720 1718 . . 1708 1712 - Hin4II* CCTTC 1702 1701 . . 1713 1717 + FokI GGATG 1726 1730 . . 1713 1717 + BseGI GGATG 1719 1717 . . 1717 1721 - BccI CCATC 1711 1712 . . 1725 1728 + TspEI AATT 1724 1728 . . 1739 1744 + Hpy166II GTNNAC 1741 1741 . . 1740 1745 + TatI WGTACW 1740 1744 . . 1740 1745 + Bsp1407I TGTACA 1740 1744 . . 1741 1744 + Csp6I GTAC 1741 1743 . . 1741 1744 + RsaI GTAC 1742 1742 . . 1744 1747 + FatI CATG 1743 1747 . . 1744 1747 + NlaIII CATG 1747 1743 . . 1744 1747 + CviAII CATG 1744 1746 . . 1747 1750 + DpnI GATC 1748 1748 . . 1747 1750 + BstKTI GATC 1749 1747 . . 1747 1750 + MboI GATC 1746 1750 . . 1750 1753 + MaeI CTAG 1750 1752 . . 1762 1772 + BsiYI* CCNNNNNNNGG 1768 1765 . . 1768 1773 + BetI* WCCGGW 1768 1772 . . 1769 1772 + HpaII CCGG 1769 1771 . . 1772 1777 + Hpy166II GTNNAC 1774 1774 . . 1776 1779 + TaiI ACGT 1779 1775 . . 1776 1779 + SetI ASST 1779 1775 . . 1776 1779 + MaeII ACGT 1776 1778 . . 1787 1790 + Csp6I GTAC 1787 1789 . . 1787 1790 + RsaI GTAC 1788 1788 . . 1792 1797 + TatI WGTACW 1792 1796 . . 1793 1796 + Csp6I GTAC 1793 1795 . . 1793 1796 + RsaI GTAC 1794 1794 . . 1790 1794 - BsrI ACTGG 1790 1788 . . 1798 1802 + TspGWI ACGGA 1813 1811 . . 1804 1814 + BsaXI ACNNNNNCTCC 1794 1791 1824 1821 1800 1805 - BciVI GTATCC 1794 1793 . . 1809 1813 + HinfI GANTC 1809 1812 . . 1809 1813 - PleI GAGTC 1803 1804 . . 1809 1813 - MlyI GAGTC 1803 1803 . . 1820 1823 + DpnI GATC 1821 1821 . . 1820 1823 + BstKTI GATC 1822 1820 . . 1820 1823 + MboI GATC 1819 1823 . . 1831 1836 + TatI WGTACW 1831 1835 . . 1832 1835 + Csp6I GTAC 1832 1834 . . 1832 1835 + RsaI GTAC 1833 1833 . . 1834 1838 + Tsp4CI* ACNGT 1836 1835 . . 1857 1862 + ApoI RAATTY 1857 1861 . . 1858 1861 + TspEI AATT 1857 1861 . . 1863 1867 + DdeI CTNAG 1863 1866 . . 1863 1867 + BspCNI CTCAG 1876 1874 . . 1863 1867 + BseMII CTCAG 1877 1875 . . 1869 1873 + DdeI CTNAG 1869 1872 . . 1872 1875 + SetI ASST 1875 1871 . . 1873 1877 + BmgT120I GGNCC 1874 1875 . . 1873 1877 + AvaII GGWCC 1873 1876 . . 1873 1877 + AsuI* GGNCC 1873 1876 . . 1889 1892 + MseI TTAA 1889 1891 . . 1891 1894 + TspEI AATT 1890 1894 . . 1900 1904 + MaeIII GTNAC 1899 1904 . . 1906 1911 + StyI CCWWGG 1906 1910 . . 1906 1911 + SecI* CCNNGG 1906 1910 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1936 1940 + MboII GAAGA 1948 1947 . . 1947 1951 + Tsp4CI* ACNGT 1949 1948 . . 1945 1949 - HphI GGTGA 1937 1936 . . 1951 1956 + MmeI TCCRAC 1976 1974 . . 1959 1964 + ApoI RAATTY 1959 1963 . . 1960 1963 + TspEI AATT 1959 1963 . . 1964 1967 + MseI TTAA 1964 1966 . . 1971 1974 + SetI ASST 1974 1970 . . 1971 1974 + CviJI RGCY 1972 1972 . . 1971 1974 + AluI AGCT 1972 1972 . . 1977 1982 + ClaI ATCGAT 1978 1980 . . 1978 1981 + TaqI TCGA 1978 1980 . . 2002 2007 + EcoRI GAATTC 2002 2006 . . 2002 2007 + ApoI RAATTY 2002 2006 . . 2003 2006 + TspEI AATT 2002 2006 . . 2006 2011 + Hpy178III* TCNNGA 2007 2009 . . 2008 2011 + TaqI TCGA 2008 2010 . . 2011 2016 + ApoI RAATTY 2011 2015 . . 2012 2015 + TspEI AATT 2011 2015 . . 2017 2020 + MseI TTAA 2017 2019 . . 2022 2027 + NspI RCATGY 2026 2022 . . 2023 2026 + FatI CATG 2022 2026 . . 2023 2026 + NlaIII CATG 2026 2022 . . 2023 2026 + CviAII CATG 2023 2025 . . 2031 2035 + MaeIII GTNAC 2030 2035 . . 2046 2050 + MaeIII GTNAC 2045 2050 . . 2052 2057 + SacI GAGCTC 2056 2052 . . 2052 2057 + Ecl136II GAGCTC 2054 2054 . . 2052 2057 + SduI GDGCHC 2056 2052 . . 2052 2057 + HgiJII* GRGCYC 2056 2052 . . 2052 2057 + HgiAI* GWGCWC 2056 2052 . . 2053 2056 + SetI ASST 2056 2052 . . 2053 2056 + CviJI RGCY 2054 2054 . . 2053 2056 + AluI AGCT 2054 2054 . . 2055 2059 + DdeI CTNAG 2055 2058 . . 2055 2059 + BspCNI CTCAG 2068 2066 . . 2055 2059 + BseMII CTCAG 2069 2067 . . 2060 2064 - MboII GAAGA 2052 2051 . . 2064 2068 + DdeI CTNAG 2064 2067 . . 2064 2068 + BspCNI CTCAG 2077 2075 . . 2064 2068 + BseMII CTCAG 2078 2076 . . 2059 2064 - BbvII* GAAGAC 2052 2056 . . 2065 2069 + Hpy188I TCNGA 2067 2066 . . 2084 2095 + TstI CACNNNNNNTCC 2075 2070 2107 2102 2085 2089 + Tsp4CI* ACNGT 2087 2086 . . 2085 2095 + BsaXI ACNNNNNCTCC 2075 2072 2105 2102 2104 2109 + AhaIII* TTTAAA 2106 2106 . . 2105 2108 + MseI TTAA 2105 2107 . . 2109 2112 + SetI ASST 2112 2108 . . 2112 2116 - TspDTI ATGAA 2102 2100 . . 2120 2123 - AciI CCGC 2120 2122 . . 2146 2149 + TaqI TCGA 2146 2148 . . 2158 2162 + TspGWI ACGGA 2173 2171 . . 2160 2164 + FokI GGATG 2173 2177 . . 2160 2164 + BseGI GGATG 2166 2164 . . 2162 2166 + TspDTI ATGAA 2177 2175 . . 2164 2169 + ApoI RAATTY 2164 2168 . . 2165 2168 + TspEI AATT 2164 2168 . . 2190 2193 + SetI ASST 2193 2189 . . 2190 2193 + CviJI RGCY 2191 2191 . . 2190 2193 + AluI AGCT 2191 2191 . . 2194 2198 + Tsp45I GTSAC 2193 2198 . . 2194 2198 + MaeIII GTNAC 2193 2198 . . 2204 2208 + TspDTI ATGAA 2219 2217 . . 2209 2213 + Tsp45I GTSAC 2208 2213 . . 2209 2213 + MaeIII GTNAC 2208 2213 . . 2211 2222 + DrdI GACNNNNNNGTC 2217 2215 . . 2213 2216 + AciI CCGC 2213 2215 . . 2219 2223 + Hpy99I CGWCG 2223 2218 . . 2229 2234 + ApoI RAATTY 2229 2233 . . 2230 2233 + TspEI AATT 2229 2233 . . 2234 2237 + MnlI CCTC 2244 2243 . . 2247 2251 + TfiI GAWTC 2247 2250 . . 2247 2251 + HinfI GANTC 2247 2250 . . 2259 2263 - BccI CCATC 2253 2254 . . 2266 2270 - SfaNI GCATC 2256 2260 . . 2276 2279 + MnlI CCTC 2286 2285 . . 2284 2289 + Hpy178III* TCNNGA 2285 2287 . . 2281 2285 - HgaI GACGC 2270 2275 . . 2293 2298 + HaeII RGCGCY 2297 2293 . . 2293 2298 + Eco47III AGCGCT 2295 2295 . . 2294 2297 + Hin6I GCGC 2294 2296 . . 2294 2297 + GlaI GCGC 2295 2295 . . 2294 2297 + HhaI GCGC 2296 2294 . . 2299 2302 + TspEI AATT 2298 2302 . . 2302 2306 + Hpy188I TCNGA 2304 2303 . . 2310 2315 + ApoI RAATTY 2310 2314 . . 2311 2314 + TspEI AATT 2310 2314 . . 2319 2322 + TspEI AATT 2318 2322 . . 2325 2330 + AhaIII* TTTAAA 2327 2327 . . 2326 2329 + MseI TTAA 2326 2328 . . 2332 2342 + MwoI GCNNNNNNNGC 2338 2335 . . 2332 2337 + Cac8I GCNNGC 2334 2334 . . 2332 2342 + BstAPI GCANNNNNTGC 2338 2335 . . 2336 2341 + BtsI GCAGTG 2343 2341 . . 2337 2343 + TspRI CASTGNN 2343 2334 . . 2341 2345 + TauI GCSGC 2344 2341 . . 2341 2345 + BlsI GCNGC 2343 2342 . . 2341 2345 + BisI GCNGC 2342 2343 . . 2342 2345 + AciI CCGC 2342 2344 . . 2343 2346 + FnuDII* CGCG 2344 2344 . . 2344 2348 - HgaI GACGC 2333 2338 . . 2364 2367 + SetI ASST 2367 2363 . . 2372 2376 + Tsp4CI* ACNGT 2374 2373 . . 2377 2381 + Hin4II* CCTTC 2387 2386 . . 2390 2399 + XmnI GAANNNNTTC 2394 2394 . . 2392 2395 + SetI ASST 2395 2391 . . 2398 2403 + Hpy178III* TCNNGA 2399 2401 . . 2421 2425 + MboII GAAGA 2433 2432 . . 2437 2440 + MseI TTAA 2437 2439 . . 2440 2444 + Tsp4CI* ACNGT 2442 2441 . . 2441 2446 + SfeI* CTRYAG 2441 2445 . . 2443 2448 + Hpy166II GTNNAC 2445 2445 . . 2443 2448 + AccI GTMKAC 2444 2446 . . 2461 2465 + TspDTI ATGAA 2476 2474 . . 2463 2467 + MboII GAAGA 2475 2474 . . 2466 2470 + MboII GAAGA 2478 2477 . . 2474 2477 + MnlI CCTC 2484 2483 . . 2480 2485 + ApoI RAATTY 2480 2484 . . 2481 2484 + TspEI AATT 2480 2484 . . 2492 2495 + TspEI AATT 2491 2495 . . 2494 2497 + MseI TTAA 2494 2496 . . 2504 2509 + SfeI* CTRYAG 2504 2508 . . 2508 2511 + SetI ASST 2511 2507 . . 2509 2513 + HphI GGTGA 2521 2520 . . 2510 2515 + Hpy166II GTNNAC 2512 2512 . . 2518 2521 + MseI TTAA 2518 2520 . . 2523 2526 + TspEI AATT 2522 2526 . . 2525 2530 + PsiI TTATAA 2527 2527 . . 2531 2536 + Hpy166II GTNNAC 2533 2533 . . 2547 2550 + TspEI AATT 2546 2550 . . 2553 2558 + ApoI RAATTY 2553 2557 . . 2554 2557 + TspEI AATT 2553 2557 . . 2565 2570 + ApoI RAATTY 2565 2569 . . 2566 2569 + TspEI AATT 2565 2569 . . 2592 2596 + Hpy188I TCNGA 2594 2593 . . 2616 2620 + BsrI ACTGG 2621 2619 . . 2617 2622 + Eco57MI CTGRAG 2638 2636 . . 2617 2622 + GsuI CTGGAG 2638 2636 . . 2622 2627 + ApoI RAATTY 2622 2626 . . 2623 2626 + TspEI AATT 2622 2626 . . 2628 2633 + XhoII RGATCY 2628 2632 . . 2628 2633 + BglII AGATCT 2628 2632 . . 2629 2632 + DpnI GATC 2630 2630 . . 2629 2632 + BstKTI GATC 2631 2629 . . 2629 2632 + MboI GATC 2628 2632 . . 2637 2640 + TspEI AATT 2636 2640 . . 2641 2645 - TspDTI ATGAA 2631 2629 . . 2647 2650 + TspEI AATT 2646 2650 . . 2657 2660 + SetI ASST 2660 2656 . . 2663 2666 + MseI TTAA 2663 2665 . . 2669 2674 + TaqII CACCCA 2685 2683 . . 2672 2686 + XcmI CCANNNNNNNNNTGG 2679 2678 . . 2672 2676 - BsrI ACTGG 2672 2670 . . 2671 2676 - BfiI ACTGGG 2666 2665 . . 2682 2687 - MmeI TCCRAC 2663 2661 . . 2698 2701 - MnlI CCTC 2691 2690 . . 2701 2706 + ApoI RAATTY 2701 2705 . . 2702 2705 + TspEI AATT 2701 2705 . . 2710 2715 + Hpy166II GTNNAC 2712 2712 . . 2718 2721 + FatI CATG 2717 2721 . . 2718 2721 + NlaIII CATG 2721 2717 . . 2718 2721 + CviAII CATG 2718 2720 . . 2722 2726 - MboII GAAGA 2714 2713 . . 2721 2726 - BbvII* GAAGAC 2714 2718 . . 2727 2732 + Eco31I GGTCTC 2733 2737 . . 2728 2732 + BsmAI GTCTC 2733 2737 . . 2725 2730 - TaqII GACCGA 2715 2713 . . 2732 2735 + MnlI CCTC 2742 2741 . . 2733 2737 + DdeI CTNAG 2733 2736 . . 2733 2737 + BspCNI CTCAG 2746 2744 . . 2733 2737 + BseMII CTCAG 2747 2745 . . 2730 2735 - BseRI GAGGAG 2721 2719 . . 2736 2741 + TatI WGTACW 2736 2740 . . 2736 2741 + ScaI AGTACT 2738 2738 . . 2737 2740 + Csp6I GTAC 2737 2739 . . 2737 2740 + RsaI GTAC 2738 2738 . . 2763 2768 + Cac8I GCNNGC 2765 2765 . . 2766 2769 + CviJI RGCY 2767 2767 . . 2772 2777 + Hpy178III* TCNNGA 2773 2775 . . 2772 2777 + BspHI TCATGA 2772 2776 . . 2773 2776 + FatI CATG 2772 2776 . . 2773 2776 + NlaIII CATG 2776 2772 . . 2773 2776 + CviAII CATG 2773 2775 . . 2771 2775 - TspDTI ATGAA 2761 2759 . . 2776 2784 + Tth111I GACNNNGTC 2779 2780 . . 2786 2790 + Hpy188I TCNGA 2788 2787 . . 2790 2795 + VspI ATTAAT 2791 2793 . . 2791 2794 + MseI TTAA 2791 2793 . . 2815 2818 + SetI ASST 2818 2814 . . 2817 2821 + DdeI CTNAG 2817 2820 . . 2840 2843 + MnlI CCTC 2850 2849 . . 2840 2850 + EcoNI CCTNNNNNAGG 2844 2845 . . 2840 2850 + BsiYI* CCNNNNNNNGG 2846 2843 . . 2848 2851 + SetI ASST 2851 2847 . . 2849 2853 + BmgT120I GGNCC 2850 2851 . . 2849 2853 + AvaII GGWCC 2849 2852 . . 2849 2853 + AsuI* GGNCC 2849 2852 . . 2847 2850 - MnlI CCTC 2840 2839 . . 2854 2857 + TspEI AATT 2853 2857 . . 2864 2868 + Hpy188I TCNGA 2866 2865 . . 2863 2868 - MmeI TCCRAC 2844 2842 . . 2871 2876 + Hpy178III* TCNNGA 2872 2874 . . 2871 2876 + BspHI TCATGA 2871 2875 . . 2872 2875 + FatI CATG 2871 2875 . . 2872 2875 + NlaIII CATG 2875 2871 . . 2872 2875 + CviAII CATG 2872 2874 . . 2873 2877 + TspDTI ATGAA 2888 2886 . . 2877 2880 + SetI ASST 2880 2876 . . 2882 2887 + Hpy178III* TCNNGA 2883 2885 . . 2901 2904 + SetI ASST 2904 2900 . . 2911 2921 + FalI AAGNNNNNCTT 2902 2897 2934 2929 2912 2918 + DraII RGGNCCY 2913 2916 . . 2913 2919 + DraII RGGNCCY 2914 2917 . . 2913 2918 + SduI GDGCHC 2917 2913 . . 2913 2918 + Bsp120I GGGCCC 2913 2917 . . 2913 2918 + NlaIV GGNNCC 2915 2915 . . 2913 2917 + BmgT120I GGNCC 2914 2915 . . 2913 2918 + HgiJII* GRGCYC 2917 2913 . . 2913 2918 + BseSI GKGCMC 2917 2913 . . 2913 2917 + AsuI* GGNCC 2913 2916 . . 2913 2918 + ApaI GGGCCC 2917 2913 . . 2914 2917 + CviJI RGCY 2915 2915 . . 2914 2917 + HaeIII GGCC 2915 2915 . . 2914 2919 + NlaIV GGNNCC 2916 2916 . . 2914 2918 + BmgT120I GGNCC 2915 2916 . . 2914 2918 + AsuI* GGNCC 2914 2917 . . 2918 2922 + Hin4II* CCTTC 2928 2927 . . 2911 2921 - FalI AAGNNNNNCTT 2934 2929 2902 2897 2933 2937 + TspDTI ATGAA 2948 2946 . . 2938 2943 + MfeI CAATTG 2938 2942 . . 2939 2942 + TspEI AATT 2938 2942 . . 2959 2964 + BsaAI YACGTR 2961 2961 . . 2960 2963 + TaiI ACGT 2963 2959 . . 2960 2963 + SetI ASST 2963 2959 . . 2960 2963 + MaeII ACGT 2960 2962 . . 2967 2971 + HphI GGTGA 2979 2978 . . 2970 2974 + PleI GAGTC 2978 2979 . . 2970 2974 + MlyI GAGTC 2979 2979 . . 2970 2974 + HinfI GANTC 2970 2973 . . 2973 2978 + Hpy178III* TCNNGA 2974 2976 . . 2993 2996 + SetI ASST 2996 2992 . . 2998 3003 + ApoI RAATTY 2998 3002 . . 2999 3002 + TspEI AATT 2998 3002 . . 3003 3008 + AhaIII* TTTAAA 3005 3005 . . 3004 3007 + MseI TTAA 3004 3006 . . 3013 3018 + MmeI TCCRAC 3038 3036 . . 3013 3017 + Hpy188I TCNGA 3015 3014 . . 3015 3019 + Hpy99I CGWCG 3019 3014 . . 3022 3027 + AhaIII* TTTAAA 3024 3024 . . 3023 3026 + MseI TTAA 3023 3025 . . 3028 3032 - SfaNI GCATC 3018 3022 . . 3047 3050 + TaqI TCGA 3047 3049 . . 3047 3052 + Hpy178III* TCNNGA 3048 3050 . . 3051 3061 + Hin4I GAYNNNNNVTC 3042 3037 3074 3069 3051 3061 - Hin4I GAYNNNNNVTC 3074 3069 3042 3037 3078 3083 + BclI TGATCA 3078 3082 . . 3079 3082 + DpnI GATC 3080 3080 . . 3079 3082 + BstKTI GATC 3081 3079 . . 3079 3082 + MboI GATC 3078 3082 . . 3082 3087 + MfeI CAATTG 3082 3086 . . 3083 3086 + TspEI AATT 3082 3086 . . 3091 3095 + MboII GAAGA 3103 3102 . . 3102 3107 + ApoI RAATTY 3102 3106 . . 3103 3106 + TspEI AATT 3102 3106 . . 3118 3122 + BlsI GCNGC 3120 3119 . . 3118 3122 + BisI GCNGC 3119 3120 . . 3118 3122 + BbvI GCAGC 3130 3134 . . 3118 3122 + TseI GCWGC 3118 3121 . . 3130 3134 + TspDTI ATGAA 3145 3143 . . 3132 3136 + MboII GAAGA 3144 3143 . . 3139 3142 + TspEI AATT 3138 3142 . . 3146 3151 + PstI CTGCAG 3150 3146 . . 3146 3151 + SfeI* CTRYAG 3146 3150 . . 3147 3150 + CviRI* TGCA 3148 3148 . . 3162 3165 + SetI ASST 3165 3161 . . 3163 3167 + HphI GGTGA 3175 3174 . . 3193 3197 + Hpy188I TCNGA 3195 3194 . . 3194 3199 + SecI* CCNNGG 3194 3198 . . 3196 3199 - MnlI CCTC 3189 3188 . . 3201 3206 + SfeI* CTRYAG 3201 3205 . . 3214 3218 + TfiI GAWTC 3214 3217 . . 3214 3218 + HinfI GANTC 3214 3217 . . 3217 3221 + Hpy188I TCNGA 3219 3218 . . 3227 3231 + TspDTI ATGAA 3242 3240 . . 3231 3234 + SetI ASST 3234 3230 . . 3231 3234 + CviJI RGCY 3232 3232 . . 3231 3234 + AluI AGCT 3232 3232 . . 3238 3243 + ClaI ATCGAT 3239 3241 . . 3239 3242 + TaqI TCGA 3239 3241 . . 3248 3251 + TspEI AATT 3247 3251 . . 3253 3258 + XhoII RGATCY 3253 3257 . . 3253 3257 + BinI* GGATC 3261 3262 . . 3254 3257 + DpnI GATC 3255 3255 . . 3254 3257 + BstKTI GATC 3256 3254 . . 3254 3257 + MboI GATC 3253 3257 . . 3256 3260 + Hpy188I TCNGA 3258 3257 . . 3257 3261 + DdeI CTNAG 3257 3260 . . 3257 3261 - BspCNI CTCAG 3249 3247 . . 3257 3261 - BseMII CTCAG 3248 3246 . . 3259 3262 - MnlI CCTC 3252 3251 . . 3279 3282 + SetI ASST 3282 3278 . . 3279 3282 + CviJI RGCY 3280 3280 . . 3279 3282 + AluI AGCT 3280 3280 . . 3280 3284 + BlsI GCNGC 3282 3281 . . 3280 3284 + BisI GCNGC 3281 3282 . . 3280 3284 + TseI GCWGC 3280 3283 . . 3280 3284 - BbvI GCAGC 3267 3271 . . 3299 3302 + SetI ASST 3302 3298 . . 3300 3304 + Hin4II* CCTTC 3310 3309 . . 3304 3309 + DsaI* CCRYGG 3304 3308 . . 3304 3309 + StyI CCWWGG 3304 3308 . . 3304 3309 + NcoI CCATGG 3304 3308 . . 3304 3309 + SecI* CCNNGG 3304 3308 . . 3305 3308 + FatI CATG 3304 3308 . . 3305 3308 + NlaIII CATG 3308 3304 . . 3305 3308 + CviAII CATG 3305 3307 . . 3313 3316 + MseI TTAA 3313 3315 . . 3319 3323 + Hpy188I TCNGA 3321 3320 . . 3317 3322 - Eco57MI CTGRAG 3302 3300 . . 3317 3322 - Eco57I CTGAAG 3302 3300 . . 3326 3330 + TspDTI ATGAA 3341 3339 . . 3331 3336 + SpeI ACTAGT 3331 3335 . . 3332 3335 + MaeI CTAG 3332 3334 . . 3346 3350 + HinfI GANTC 3346 3349 . . 3348 3353 + SmlI CTYRAG 3348 3352 . . 3349 3354 + Hpy178III* TCNNGA 3350 3352 . . 3346 3350 - PleI GAGTC 3340 3341 . . 3346 3350 - MlyI GAGTC 3340 3340 . . 3348 3353 - Bce83I* CTTGAG 3333 3331 . . 3353 3356 - MnlI CCTC 3346 3345 . . 3359 3364 + ApoI RAATTY 3359 3363 . . 3360 3363 + TspEI AATT 3359 3363 . . 3370 3374 + BslFI GGGAC 3384 3388 . . 3368 3371 - AciI CCGC 3368 3370 . . 3368 3372 - FauI CCCGC 3361 3363 . . 3379 3382 - MnlI CCTC 3372 3371 . . 3383 3389 + TspRI CASTGNN 3389 3380 . . 3397 3400 + MseI TTAA 3397 3399 . . 3398 3403 + TsoI TARCCA 3414 3412 . . 3405 3410 + Hpy178III* TCNNGA 3406 3408 . . 3405 3410 + BspHI TCATGA 3405 3409 . . 3401 3405 - BsrI ACTGG 3401 3399 . . 3406 3409 + FatI CATG 3405 3409 . . 3406 3409 + NlaIII CATG 3409 3405 . . 3406 3409 + CviAII CATG 3406 3408 . . 3407 3411 + TspDTI ATGAA 3422 3420 . . 3421 3425 + TauI GCSGC 3424 3421 . . 3421 3425 + BlsI GCNGC 3423 3422 . . 3421 3425 + BisI GCNGC 3422 3423 . . 3418 3422 - SfaNI GCATC 3408 3412 . . 3423 3426 + CviJI RGCY 3424 3424 . . 3421 3424 - AciI CCGC 3421 3423 . . 3433 3437 + MaeIII GTNAC 3432 3437 . . 3442 3445 + MseI TTAA 3442 3444 . . 3452 3456 + Hpy188I TCNGA 3454 3453 . . 3463 3466 + MseI TTAA 3463 3465 . . 3464 3469 + TsoI TARCCA 3480 3478 . . 3499 3502 + SetI ASST 3502 3498 . . 3501 3504 + Csp6I GTAC 3501 3503 . . 3501 3504 + RsaI GTAC 3502 3502 . . 3510 3515 + BciVI GTATCC 3521 3520 . . 3514 3517 + MnlI CCTC 3524 3523 . . 3514 3524 + EcoNI CCTNNNNNAGG 3518 3519 . . 3514 3524 + BsiYI* CCNNNNNNNGG 3520 3517 . . 3516 3521 + Hpy178III* TCNNGA 3517 3519 . . 3518 3523 + Eco57MI CTGRAG 3539 3537 . . 3518 3523 + Eco57I CTGAAG 3539 3537 . . 3520 3524 - Hin4II* CCTTC 3514 3513 . . 3524 3527 + CviJI RGCY 3525 3525 . . 3526 3530 + DdeI CTNAG 3526 3529 . . 3530 3534 + MaeIII GTNAC 3529 3534 . . 3526 3530 - BspCNI CTCAG 3518 3516 . . 3526 3530 - BseMII CTCAG 3517 3515 . . 3544 3549 + SspI AATATT 3546 3546 . . 3575 3578 + MseI TTAA 3575 3577 . . 3581 3585 - BceAI ACGGC 3566 3568 . . 3589 3594 + Hpy166II GTNNAC 3591 3591 . . 3589 3594 + HindII GTYRAC 3591 3591 . . 3612 3616 + HgaI GACGC 3621 3626 . . 3631 3634 + TspEI AATT 3630 3634 . . 3638 3642 + TspDTI ATGAA 3653 3651 . . 3640 3652 + PsrI GAACNNNNNNTAC 3632 3627 3664 3659 3642 3645 + SetI ASST 3645 3641 . . 3643 3647 + BssKI CCNGG 3642 3647 . . 3643 3647 + BseBI CCWGG 3644 3645 . . 3643 3647 + ScrFI CCNGG 3644 3645 . . 3643 3647 + EcoRII CCWGG 3642 3647 . . 3653 3659 + TspRI CASTGNN 3659 3650 . . 3657 3661 + TfiI GAWTC 3657 3660 . . 3657 3661 + HinfI GANTC 3657 3660 . . 3663 3668 + ApoI RAATTY 3663 3667 . . 3664 3667 + TspEI AATT 3663 3667 . . 3675 3679 - BccI CCATC 3669 3670 . . 3685 3689 + Hpy188I TCNGA 3687 3686 . . 3697 3701 + BssKI CCNGG 3696 3701 . . 3697 3700 + HpaII CCGG 3697 3699 . . 3697 3702 + SecI* CCNNGG 3697 3701 . . 3697 3701 + ScrFI CCNGG 3698 3699 . . 3697 3701 + CauII* CCSGG 3698 3699 . . 3708 3713 + XhoII RGATCY 3708 3712 . . 3708 3712 + BinI* GGATC 3716 3717 . . 3706 3709 - MnlI CCTC 3699 3698 . . 3709 3712 + DpnI GATC 3710 3710 . . 3709 3712 + BstKTI GATC 3711 3709 . . 3709 3712 + MboI GATC 3708 3712 . . 3716 3719 + CviRI* TGCA 3717 3717 . . 3728 3732 + TspDTI ATGAA 3743 3741 . . 3761 3767 + TspRI CASTGNN 3767 3758 . . 3760 3764 - BsrI ACTGG 3760 3758 . . 3765 3770 + XhoII RGATCY 3765 3769 . . 3765 3770 + NlaIV GGNNCC 3767 3767 . . 3765 3770 + BamHI GGATCC 3765 3769 . . 3765 3769 + BinI* GGATC 3773 3774 . . 3766 3769 + DpnI GATC 3767 3767 . . 3766 3769 + BstKTI GATC 3768 3766 . . 3766 3769 + MboI GATC 3765 3769 . . 3769 3775 + Bpu10I CCTNAGC 3770 3773 . . 3770 3774 + DdeI CTNAG 3770 3773 . . 3766 3770 - BinI* GGATC 3760 3761 . . 3781 3785 + TfiI GAWTC 3781 3784 . . 3781 3785 + HinfI GANTC 3781 3784 . . 3781 3791 + Hin4I GAYNNNNNVTC 3772 3767 3804 3799 3781 3791 - Hin4I GAYNNNNNVTC 3804 3799 3772 3767 3799 3802 + Csp6I GTAC 3799 3801 . . 3799 3802 + RsaI GTAC 3800 3800 . . 3813 3816 + TaiI ACGT 3816 3812 . . 3813 3816 + SetI ASST 3816 3812 . . 3813 3816 + MaeII ACGT 3813 3815 . . 3815 3819 + Tsp45I GTSAC 3814 3819 . . 3815 3819 + MaeIII GTNAC 3814 3819 . . 3817 3827 + Hin4I GAYNNNNNVTC 3808 3803 3840 3835 3817 3827 - Hin4I GAYNNNNNVTC 3840 3835 3808 3803 3826 3830 - HphI GGTGA 3818 3817 . . 3836 3841 + BspLU11I* ACATGT 3836 3840 . . 3836 3841 + NspI RCATGY 3840 3836 . . 3836 3841 + AflIII ACRYGT 3836 3840 . . 3837 3840 + FatI CATG 3836 3840 . . 3837 3840 + NlaIII CATG 3840 3836 . . 3837 3840 + CviAII CATG 3837 3839 . . 3831 3841 - BaeI ACNNNNGTAYC 3856 3851 3823 3818 3841 3845 - MboII GAAGA 3833 3832 . . 3840 3845 - BbvII* GAAGAC 3833 3837 . . 3847 3851 - MboII GAAGA 3839 3838 . . 3846 3851 - BbvII* GAAGAC 3839 3843 . . 3856 3860 + BccI CCATC 3864 3865 . . 3859 3863 - MboII GAAGA 3851 3850 . . 3865 3875 + MwoI GCNNNNNNNGC 3871 3868 . . 3868 3872 - MboII GAAGA 3860 3859 . . 3869 3874 - Eco57MI CTGRAG 3854 3852 . . 3869 3874 - Eco57I CTGAAG 3854 3852 . . 3888 3892 + MboII GAAGA 3900 3899 . . 3893 3896 + TspEI AATT 3892 3896 . . 3899 3902 + SetI ASST 3902 3898 . . 3899 3902 + CviJI RGCY 3900 3900 . . 3899 3902 + AluI AGCT 3900 3900 . . 3923 3928 + NdeI CATATG 3924 3926 . . 3934 3939 + MfeI CAATTG 3934 3938 . . 3935 3938 + TspEI AATT 3934 3938 . . 3940 3944 + Tsp4CI* ACNGT 3942 3941 . . 3942 3947 + TatI WGTACW 3942 3946 . . 3943 3946 + Csp6I GTAC 3943 3945 . . 3943 3946 + RsaI GTAC 3944 3944 . . 3952 3955 + FatI CATG 3951 3955 . . 3952 3955 + NlaIII CATG 3955 3951 . . 3952 3955 + CviAII CATG 3952 3954 . . 3962 3965 + SetI ASST 3965 3961 . . 3963 3966 + MnlI CCTC 3973 3972 . . 3963 3969 + SauI* CCTNAGG 3964 3967 . . 3964 3968 + DdeI CTNAG 3964 3967 . . 3964 3968 + BspCNI CTCAG 3977 3975 . . 3964 3968 + BseMII CTCAG 3978 3976 . . 3965 3970 + Hpy178III* TCNNGA 3966 3968 . . 3968 3972 + BinI* GGATC 3976 3977 . . 3969 3972 + DpnI GATC 3970 3970 . . 3969 3972 + BstKTI GATC 3971 3969 . . 3969 3972 + MboI GATC 3968 3972 . . 3982 3987 + BsmI GAATGC 3988 3986 . . 3998 4001 + FatI CATG 3997 4001 . . 3998 4001 + NlaIII CATG 4001 3997 . . 3998 4001 + CviAII CATG 3998 4000 . . 4003 4008 + KpnI GGTACC 4007 4003 . . 4003 4008 + NlaIV GGNNCC 4005 4005 . . 4003 4008 + HgiCI* GGYRCC 4003 4007 . . 4003 4008 + Acc65I GGTACC 4003 4007 . . 4004 4007 + Csp6I GTAC 4004 4006 . . 4004 4007 + RsaI GTAC 4005 4005 . . 4022 4027 - TaqII CACCCA 4012 4010 . . 4032 4036 + Hpy188I TCNGA 4034 4033 . . 4045 4050 + ApoI RAATTY 4045 4049 . . 4046 4049 + TspEI AATT 4045 4049 . . 4053 4056 + SetI ASST 4056 4052 . . 4053 4056 + CviJI RGCY 4054 4054 . . 4053 4056 + AluI AGCT 4054 4054 . . 4075 4078 + TspEI AATT 4074 4078 . . 4112 4117 + Hpy178III* TCNNGA 4113 4115 . . 4117 4122 + AflIII ACRYGT 4117 4121 . . 4119 4122 + TaiI ACGT 4122 4118 . . 4119 4122 + SetI ASST 4122 4118 . . 4119 4122 + MaeII ACGT 4119 4121 . . 4127 4130 + TspEI AATT 4126 4130 . . 4129 4132 + MseI TTAA 4129 4131 . . 4136 4141 + SspI AATATT 4138 4138 . . 4144 4148 + MaeIII GTNAC 4143 4148 . . 4147 4151 + Tsp4CI* ACNGT 4149 4148 . . 4154 4159 + GsaI CCCAGC 4158 4154 . . 4154 4159 + BseYI CCCAGC 4154 4158 . . 4177 4180 + TspEI AATT 4176 4180 . . 4183 4187 - MboII GAAGA 4175 4174 . . 4205 4208 + AciI CCGC 4205 4207 . . 4217 4220 + MnlI CCTC 4227 4226 . . 4244 4247 + FatI CATG 4243 4247 . . 4244 4247 + NlaIII CATG 4247 4243 . . 4244 4247 + CviAII CATG 4244 4246 . . 4260 4266 + TspRI CASTGNN 4266 4257 . . 4262 4266 + DdeI CTNAG 4262 4265 . . 4264 4268 + PleI GAGTC 4272 4273 . . 4264 4268 + MlyI GAGTC 4273 4273 . . 4264 4268 + HinfI GANTC 4264 4267 . . 4262 4266 - BspCNI CTCAG 4254 4252 . . 4262 4266 - BseMII CTCAG 4253 4251 . . 4267 4270 + TaqI TCGA 4267 4269 . . 4271 4277 + PpuMI RGGWCCY 4272 4275 . . 4271 4277 + DraII RGGNCCY 4272 4275 . . 4272 4276 + BmgT120I GGNCC 4273 4274 . . 4272 4276 + AvaII GGWCC 4272 4275 . . 4272 4276 + AsuI* GGNCC 4272 4275 . . 4269 4273 - Hin4II* CCTTC 4263 4262 . . 4274 4277 + SetI ASST 4277 4273 . . 4275 4279 + Hin4II* CCTTC 4285 4284 . . 4278 4282 + Hpy188I TCNGA 4280 4279 . . 4279 4284 + Eco57MI CTGRAG 4300 4298 . . 4279 4284 + Eco57I CTGAAG 4300 4298 . . 4281 4285 + MboII GAAGA 4293 4292 . . 4286 4289 + SetI ASST 4289 4285 . . 4295 4298 + SetI ASST 4298 4294 . . 4303 4307 + TfiI GAWTC 4303 4306 . . 4303 4307 + HinfI GANTC 4303 4306 . . 4314 4319 + ApoI RAATTY 4314 4318 . . 4315 4318 + TspEI AATT 4314 4318 . . 4334 4337 + SetI ASST 4337 4333 . . 4334 4337 + CviJI RGCY 4335 4335 . . 4334 4337 + AluI AGCT 4335 4335 . . 4343 4346 + DpnI GATC 4344 4344 . . 4343 4346 + BstKTI GATC 4345 4343 . . 4343 4346 + MboI GATC 4342 4346 . . 4343 4347 - BinI* GGATC 4337 4338 . . 4348 4352 + Tsp45I GTSAC 4347 4352 . . 4348 4352 + MaeIII GTNAC 4347 4352 . . 4345 4349 - TspGWI ACGGA 4335 4333 . . 4351 4355 + TspGWI ACGGA 4366 4364 . . 4355 4358 + TaiI ACGT 4358 4354 . . 4355 4358 + SetI ASST 4358 4354 . . 4355 4358 + MaeII ACGT 4355 4357 . . 4359 4362 + CviRI* TGCA 4360 4360 . . 4360 4365 + NspI RCATGY 4364 4360 . . 4361 4364 + FatI CATG 4360 4364 . . 4361 4364 + NlaIII CATG 4364 4360 . . 4361 4364 + CviAII CATG 4361 4363 . . 4369 4379 + BsiYI* CCNNNNNNNGG 4375 4372 . . 4381 4386 + Hpy166II GTNNAC 4383 4383 . . 4381 4386 + BssNAI GTATAC 4383 4383 . . 4381 4386 + AccI GTMKAC 4382 4384 . . 4385 4388 + SetI ASST 4388 4384 . . 4390 4394 + Hpy188I TCNGA 4392 4391 . . 4396 4399 + MseI TTAA 4396 4398 . . 4405 4408 + TaiI ACGT 4408 4404 . . 4405 4408 + SetI ASST 4408 4404 . . 4405 4408 + MaeII ACGT 4405 4407 . . 4412 4416 + Hpy188I TCNGA 4414 4413 . . 4411 4416 - MmeI TCCRAC 4392 4390 . . 4428 4432 - MboII GAAGA 4420 4419 . . 4435 4438 + TspEI AATT 4434 4438 . . 4455 4460 - Eco57MI CTGRAG 4440 4438 . . 4455 4460 - Eco57I CTGAAG 4440 4438 . . 4464 4467 - MnlI CCTC 4457 4456 . . 4478 4489 + CspCI CAANNNNNGTGG 4466 4464 4501 4499 4489 4492 - MnlI CCTC 4482 4481 . . 4494 4497 + TspEI AATT 4493 4497 . . 4496 4501 + PsiI TTATAA 4498 4498 . . 4500 4505 + HindIII AAGCTT 4500 4504 . . 4501 4504 + SetI ASST 4504 4500 . . 4501 4504 + CviJI RGCY 4502 4502 . . 4501 4504 + AluI AGCT 4502 4502 . . 4504 4509 + AhaIII* TTTAAA 4506 4506 . . 4505 4508 + MseI TTAA 4505 4507 . . 4511 4515 + TfiI GAWTC 4511 4514 . . 4511 4515 + HinfI GANTC 4511 4514 . . 4523 4526 + SetI ASST 4526 4522 . . 4523 4526 + CviJI RGCY 4524 4524 . . 4523 4526 + AluI AGCT 4524 4524 . . 4536 4540 + FokI GGATG 4549 4553 . . 4536 4540 + BseGI GGATG 4542 4540 . . 4545 4549 + Hpy188I TCNGA 4547 4546 . . 4549 4553 + Tsp4CI* ACNGT 4551 4550 . . 4561 4565 - HgaI GACGC 4550 4555 . . 4576 4580 - BslFI GGGAC 4561 4565 . . 4601 4604 + TspEI AATT 4600 4604 . . 4607 4613 + TspRI CASTGNN 4613 4604 . . 4610 4613 + CviRI* TGCA 4611 4611 . . 4607 4612 - BtsI GCAGTG 4606 4604 . . 4617 4622 + NlaIV GGNNCC 4619 4619 . . 4621 4631 + BsiYI* CCNNNNNNNGG 4627 4624 . . 4625 4628 + DpnI GATC 4626 4626 . . 4625 4628 + BstKTI GATC 4627 4625 . . 4625 4628 + MboI GATC 4624 4628 . . 4627 4632 + Hpy178III* TCNNGA 4628 4630 . . 4646 4649 + Csp6I GTAC 4646 4648 . . 4646 4649 + RsaI GTAC 4647 4647 . . 4650 4655 + NspI RCATGY 4654 4650 . . 4650 4655 + Cac8I GCNNGC 4652 4652 . . 4650 4655 + SphI GCATGC 4654 4650 . . 4651 4654 + FatI CATG 4650 4654 . . 4651 4654 + NlaIII CATG 4654 4650 . . 4651 4654 + CviAII CATG 4651 4653 . . 4654 4664 + MwoI GCNNNNNNNGC 4660 4657 . . 4646 4658 - BarI GAAGNNNNNNTAC 4670 4665 4638 4633 4657 4661 - MboII GAAGA 4649 4648 . . 4663 4668 + Cac8I GCNNGC 4665 4665 . . 4663 4668 + BmtI GCTAGC 4667 4663 . . 4663 4668 + NheI GCTAGC 4663 4667 . . 4664 4667 + MaeI CTAG 4664 4666 . . 4670 4673 + Csp6I GTAC 4670 4672 . . 4670 4673 + RsaI GTAC 4671 4671 . . 4667 4670 - AciI CCGC 4667 4669 . . 4680 4683 + CviRI* TGCA 4681 4681 . . 4684 4689 + ApoI RAATTY 4684 4688 . . 4685 4688 + TspEI AATT 4684 4688 . . 4690 4694 + DdeI CTNAG 4690 4693 . . 4696 4701 + ApoI RAATTY 4696 4700 . . 4697 4700 + TspEI AATT 4696 4700 . . 4720 4723 + TspEI AATT 4719 4723 . . 4730 4733 + SetI ASST 4733 4729 . . 4730 4733 + CviJI RGCY 4731 4731 . . 4730 4733 + AluI AGCT 4731 4731 . . 4732 4735 + MaeI CTAG 4732 4734 . . 4734 4737 + SetI ASST 4737 4733 . . 4746 4749 + SetI ASST 4749 4745 . . 4747 4750 + MnlI CCTC 4757 4756 . . 4750 4754 + Hin4II* CCTTC 4760 4759 . . 4754 4757 + MaeI CTAG 4754 4756 . . 4756 4759 + SetI ASST 4759 4755 . . 4764 4767 + TaqI TCGA 4764 4766 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 767 4003 # # AccI: # [GTMKAC] # 71 369 388 483 1521 1938 # # AciI: # [CCGC] # 53 93 103 129 462 784 # 1024 1026 1096 # # AflIII: # [ACRYGT] # 14 281 469 653 3353 # # AhaIII*: # [TTTAAA] # 19 221 264 678 1482 2106 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 612 661 3433 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 1500 3236 # # AloI: # [GAACNNNNNNTCC] # 32 32 32 110 326 504 # 3734 # # AluI: # [AGCT] # 9 15 22 39 47 48 # 80 82 137 154 167 207 # 281 310 339 620 1041 1172 # # AlwNI: # [CAGNNNCTG] # 347 4423 # # ApaI: # [GGGCCC] # 1853 2917 # # ApoI: # [RAATTY] # 9 12 12 29 36 43 # 57 65 66 73 74 79 # 81 104 153 170 210 257 # 265 269 297 304 370 382 # 600 753 # # AsuI*: # [GGNCC] # 1 64 97 365 498 630 # 781 976 1358 # # AsuII: # [TTCGAA] # 207 652 3911 # # AvaII: # [GGWCC] # 97 365 498 630 781 976 # 1423 # # BaeI: # [ACNNNNGTAYC] # 33 914 3823 # # BalI: # [TGGCCA] # 1333 3437 # # BamHI: # [GGATCC] # 1005 3765 # # BarI: # [GAAGNNNNNNTAC] # 32 100 4638 # # BbvCI: # [CCTCAGC] # 123 4647 # # BbvI: # [GCAGC] # 3 64 137 180 278 1034 # 1503 1571 # # BbvII*: # [GAAGAC] # 6 567 662 931 1119 1485 # # BccI: # [CCATC] # 78 195 220 399 468 542 # 546 906 1416 # # Bce83I*: # [CTTGAG] # 423 1437 2910 # # BceAI: # [ACGGC] # 1204 1433 2133 # # BciVI: # [GTATCC] # 147 1249 1647 1727 # # BclI: # [TGATCA] # 1032 1692 2046 # # BetI*: # [WCCGGW] # 1768 3002 # # BfiI: # [ACTGGG] # 2104 2666 # # BglII: # [AGATCT] # 1059 1569 2142 # # BinI*: # [GGATC] # 13 44 203 361 433 455 # 3261 # # BisI: # [GCNGC] # 3 39 141 162 194 253 # 777 794 1059 1348 # # BlsI: # [GCNGC] # 3 39 141 162 195 253 # 777 794 1059 1347 # # BmgT120I: # [GGNCC] # 1 64 97 365 497 630 # 782 976 1358 # # BmtI: # [GCTAGC] # 103 4667 # # Bpu10I: # [CCTNAGC] # 123 1000 3647 # # BsaAI: # [YACGTR] # 1809 2961 # # BsaBI: # [GATNNNNATC] # 1603 3167 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 251 504 1260 # 2665 # # BseBI: # [CCWGG] # 682 1126 2962 # # BseGI: # [GGATG] # 96 118 191 228 447 499 # 815 2376 # # BseMII: # [CTCAG] # 9 137 192 268 269 275 # 299 350 461 501 517 669 # 823 # # BseRI: # [GAGGAG] # 2049 2721 # # BseSI: # [GKGCMC] # 1853 2917 # # BseYI: # [CCCAGC] # 616 4154 # # BsgI: # [GTGCAG] # 719 4051 # # BsiYI*: # [CCNNNNNNNGG] # 143 252 625 674 855 1078 # 1143 # # BslFI: # [GGGAC] # 97 209 767 1177 2520 # # BsmAI: # [GTCTC] # 374 1079 1280 2037 # # BsmI: # [GAATGC] # 167 782 3821 # # Bsp120I: # [GGGCCC] # 1857 2913 # # Bsp1407I: # [TGTACA] # 249 1491 3030 # # BspCNI: # [CTCAG] # 9 136 192 268 269 277 # 301 350 459 503 516 669 # 821 # # BspHI: # [TCATGA] # 99 534 1365 2772 # # BspLU11I*: # [ACATGT] # 483 934 3353 # # BspMI: # [ACCTGC] # 769 4001 # # BsrDI: # [GCAATG] # 60 803 3907 # # BsrI: # [ACTGG] # 51 359 428 456 729 831 # 906 1010 # # BssKI: # [CCNGG] # 54 680 1074 2962 # # BssNAI: # [GTATAC] # 71 387 853 3459 # # BstAPI: # [GCANNNNNTGC] # 55 2283 2432 # # BstKTI: # [GATC] # 27 30 40 57 73 77 # 143 175 203 282 374 450 # 455 617 809 958 # # BtrI: # [CACGTC] # 17 4753 # # BtsI: # [GCAGTG] # 164 2263 2343 # # Cac8I: # [GCNNGC] # 13 51 105 431 821 1462 # 1887 # # CauII*: # [CCSGG] # 1072 3698 # # CfrI: # [YGGCCR] # 573 758 3439 # # ClaI: # [ATCGAT] # 73 519 1261 1386 1531 # # Csp6I: # [GTAC] # 6 24 39 46 61 100 # 144 250 298 642 764 905 # 1491 # # CspCI: # [CAANNNNNGTGG] # 35 269 4466 # # CviAII: # [CATG] # 46 55 99 101 115 117 # 119 219 246 279 290 431 # 433 484 695 1041 # # CviJI: # [RGCY] # 7 9 15 22 22 39 # 47 48 80 82 87 88 # 90 93 96 101 104 114 # 132 137 144 148 154 167 # 207 220 281 317 339 375 # 429 576 # # CviRI*: # [TGCA] # 10 26 40 70 84 89 # 141 251 435 569 643 811 # 1601 # # DdeI: # [CTNAG] # 6 9 80 84 123 186 # 194 244 268 269 298 322 # 350 428 440 669 800 # # DpnI: # [GATC] # 27 30 40 57 73 76 # 144 175 203 282 374 450 # 455 617 809 958 # # DraII: # [RGGNCCY] # 1 498 781 1358 2132 # # DrdI: # [GACNNNNNNGTC] # 2217 2553 # # DsaI*: # [CCRYGG] # 1466 3304 # # Ecl136II: # [GAGCTC] # 437 1617 2716 # # Eco31I: # [GGTCTC] # 2037 2733 # # Eco47III: # [AGCGCT] # 2295 2475 # # Eco57I: # [CTGAAG] # 140 237 315 330 446 3302 # # Eco57MI: # [CTGRAG] # 140 237 315 330 446 664 # 1256 1382 # # EcoNI: # [CCTNNNNNAGG] # 674 1252 2844 # # EcoP15I: # [CAGCAG] # 477 4293 # # EcoRI: # [GAATTC] # 774 1228 2768 # # EcoRII: # [CCWGG] # 680 1128 2962 # # EcoRV: # [GATATC] # 1478 3292 # # Esp3I: # [CGTCTC] # 1453 3317 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 1305 1565 1836 # # FatI: # [CATG] # 46 55 99 101 115 117 # 120 219 246 279 290 431 # 433 483 695 1041 # # FauI: # [CCCGC] # 1031 1409 2330 # # FnuDII*: # [CGCG] # 2344 2426 # # FokI: # [GGATG] # 76 105 211 221 447 499 # 835 2376 # # GlaI: # [GCGC] # 172 445 1678 2475 # # GsaI: # [CCCAGC] # 612 4158 # # GsuI: # [CTGGAG] # 1256 1382 2132 # # HaeII: # [RGCGCY] # 619 1678 2473 # # HaeIII: # [GGCC] # 575 758 1582 1855 # # HgaI: # [GACGC] # 63 220 524 929 1288 1746 # # HgiAI*: # [GWGCWC] # 439 1617 2714 # # HgiCI*: # [GGYRCC] # 767 4003 # # HgiJII*: # [GRGCYC] # 439 861 1617 1853 # # HhaI: # [GCGC] # 172 446 1678 2474 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 4 32 # 32 32 32 32 694 698 # 736 930 1548 # # Hin4II*: # [CCTTC] # 10 22 109 204 382 446 # 475 541 685 749 1147 # # Hin6I: # [GCGC] # 172 444 1678 2476 # # HindII: # [GTYRAC] # 161 178 705 1179 2547 # # HindIII: # [AAGCTT] # 270 4500 # # HinfI: # [GANTC] # 39 40 115 124 132 144 # 168 182 208 244 259 287 # 311 438 483 723 873 # # HpaI: # [GTTAAC] # 339 705 3726 # # HpaII: # [CCGG] # 572 1073 1197 1928 # # HphI: # [GGTGA] # 196 458 584 643 781 952 # 1156 # # Hpy166II: # [GTNNAC] # 21 31 33 67 71 76 # 120 161 178 179 259 362 # 387 483 671 792 879 # # Hpy178III*: # [TCNNGA] # 11 56 74 88 91 97 # 99 111 114 142 147 226 # 278 302 323 374 449 515 # 1273 # # Hpy188I: # [TCNGA] # 6 16 22 24 36 39 # 48 49 63 71 78 90 # 112 133 133 149 180 194 # 195 214 223 233 237 246 # 290 347 369 388 585 # # Hpy99I: # [CGWCG] # 796 1751 2223 # # KpnI: # [GGTACC] # 763 4007 # # Ksp632I*: # [CTCTTC] # 87 264 505 734 3180 # # MaeI: # [CTAG] # 16 22 50 68 119 180 # 265 491 645 1332 1582 # # MaeII: # [ACGT] # 16 50 236 306 365 839 # 853 921 1184 # # MaeIII: # [GTNAC] # 15 15 97 131 148 204 # 285 329 336 423 560 1003 # 1224 # # MboI: # [GATC] # 27 30 40 57 73 74 # 146 175 203 282 374 450 # 455 617 809 958 # # MboII: # [GAAGA] # 2 3 3 6 9 12 # 27 40 41 42 54 74 # 90 93 104 118 121 127 # 170 172 229 236 275 371 # 381 389 417 475 689 # # MfeI: # [CAATTG] # 144 176 836 852 2762 # # MlyI: # [GAGTC] # 361 497 933 1176 1803 # # MmeI: # [TCCRAC] # 181 194 378 687 803 1173 # 1354 # # MnlI: # [CCTC] # 10 13 19 24 25 26 # 28 39 42 51 63 93 # 94 98 141 147 152 153 # 175 181 198 207 230 254 # 274 275 339 405 1014 # # MseI: # [TTAA] # 12 19 21 24 24 27 # 36 38 45 53 57 60 # 60 65 75 78 84 88 # 96 109 111 112 128 145 # 147 150 157 198 205 206 # 213 221 265 267 290 330 # 554 # # MslI: # [CAYNNNNRTG] # 294 4476 # # MwoI: # [GCNNNNNNNGC] # 9 15 46 98 110 388 # 789 1533 1782 # # NcoI: # [CCATGG] # 1466 3304 # # NdeI: # [CATATG] # 846 3924 # # NheI: # [GCTAGC] # 107 4663 # # NlaIII: # [CATG] # 46 55 99 101 115 116 # 117 219 246 279 290 431 # 433 487 695 1041 # # NlaIV: # [GGNNCC] # 1 97 151 238 614 783 # 851 2035 # # NspI: # [RCATGY] # 116 290 487 524 1539 1814 # # PleI: # [GAGTC] # 362 498 932 1175 1803 # # PpiI: # [GAACNNNNNCTC] # 32 504 4234 # # PpuMI: # [RGGWCCY] # 498 781 3491 # # PsiI: # [TTATAA] # 272 1971 2527 # # PsrI: # [GAACNNNNNNTAC] # 32 1106 3632 # # PstI: # [CTGCAG] # 1513 1620 1637 # # RsaI: # [GTAC] # 6 24 39 46 61 99 # 144 251 298 642 764 905 # 1491 # # SacI: # [GAGCTC] # 439 1617 2714 # # SapI: # [GCTCTTC] # 87 1239 3444 # # SauI*: # [CCTNAGG] # 806 3964 # # ScaI: # [AGTACT] # 2032 2738 # # ScrFI: # [CCNGG] # 54 682 1072 2962 # # SduI: # [GDGCHC] # 439 861 1617 1853 # # SecI*: # [CCNNGG] # 110 393 680 1073 1226 1288 # # SetI: # [ASST] # 4 9 9 10 11 12 # 12 15 19 20 20 21 # 22 24 28 29 30 33 # 33 39 47 48 56 57 # 59 63 63 64 66 69 # 75 81 82 83 86 91 # 96 96 99 108 116 143 # 144 149 155 158 169 171 # 174 181 200 207 246 320 # 348 # # SfaNI: # [GCATC] # 390 615 762 1362 1641 # # SfeI*: # [CTRYAG] # 55 63 642 932 1509 1569 # # SmlI: # [CTYRAG] # 438 1422 2910 # # SpeI: # [ACTAGT] # 789 1439 2542 # # SphI: # [GCATGC] # 116 4654 # # SspI: # [AATATT] # 210 592 632 1080 2256 # # StyI: # [CCWWGG] # 1398 1466 1906 # # TaiI: # [ACGT] # 19 50 236 306 362 839 # 853 921 1184 # # TaqI: # [TCGA] # 6 12 30 60 73 75 # 138 192 207 231 353 444 # 497 523 901 1028 # # TaqII: # [CACCCA] # 30 284 758 1297 2401 # # TatI: # [WGTACW] # 39 52 249 828 905 1206 # 1491 # # TauI: # [GCSGC] # 1080 1346 2344 # # TfiI: # [GAWTC] # 40 115 124 144 168 208 # 259 287 443 522 620 873 # 967 # # TseI: # [GCWGC] # 3 39 162 193 253 1059 # 1490 1571 # # TsoI: # [TARCCA] # 66 1290 3414 # # Tsp45I: # [GTSAC] # 15 336 423 533 854 1003 # 1606 # # Tsp4CI*: # [ACNGT] # 18 60 68 96 96 101 # 113 131 138 138 185 207 # 215 219 229 233 287 334 # 402 1500 # # TspDTI: # [ATGAA] # 10 18 36 42 60 74 # 75 81 90 97 98 99 # 123 127 130 155 183 197 # 231 257 303 353 427 477 # 1027 # # TspEI: # [AATT] # 7 8 9 10 11 12 # 12 12 12 14 20 22 # 22 23 24 29 29 31 # 31 32 33 33 33 36 # 36 38 42 43 50 51 # 52 53 55 57 59 60 # 60 65 68 69 71 77 # 79 84 84 84 85 107 # 109 111 112 115 120 133 # 138 152 152 153 162 176 # 210 229 271 393 # # TspGWI: # [ACGGA] # 31 93 360 404 1720 2162 # # TspRI: # [CASTGNN] # 108 157 270 347 499 896 # 1046 1447 # # TstI: # [CACNNNNNNTCC] # 6 32 32 2037 2663 # # Tth111I: # [GACNNNGTC] # 876 1903 1991 # # VspI: # [ATTAAT] # 345 845 1601 1979 # # XcmI: # [CCANNNNNNNNNTGG] # 2091 2679 # # XhoII: # [RGATCY] # 57 455 625 1005 1059 1569 # # XmnI: # [GAANNNNTTC] # 827 1567 2376 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4770 # Reported_sequences: 1 # Reported_hitcount: 1088 #---------------------------------------