Restriction Fragments for LIP2/YLR239C



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The currently selected gene/sequence is :

LIP2/YLR239C on chromosome XII from coordinates 617318 to 616332.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.13988.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + TaqI TCGA 4 6 . . 7 10 + SetI ASST 10 6 . . 18 22 - BsrI ACTGG 18 16 . . 22 26 - TspGWI ACGGA 12 10 . . 28 31 + CviRI* TGCA 29 29 . . 45 50 + SfeI* CTRYAG 45 49 . . 74 77 + MnlI CCTC 84 83 . . 82 87 + SmlI CTYRAG 82 86 . . 82 87 - Bce83I* CTTGAG 67 65 . . 97 101 - FokI GGATG 83 87 . . 97 101 - BseGI GGATG 96 94 . . 110 113 + MnlI CCTC 120 119 . . 110 116 + Bpu10I CCTNAGC 111 114 . . 110 116 + BbvCI CCTCAGC 111 114 . . 111 115 + DdeI CTNAG 111 114 . . 111 115 + BspCNI CTCAG 124 122 . . 111 115 + BseMII CTCAG 125 123 . . 115 118 + Hin6I GCGC 115 117 . . 115 118 + GlaI GCGC 116 116 . . 115 118 + HhaI GCGC 117 115 . . 124 128 + DdeI CTNAG 124 127 . . 124 128 + BspCNI CTCAG 137 135 . . 124 128 + BseMII CTCAG 138 136 . . 125 129 + Hpy188I TCNGA 127 126 . . 137 140 + TspEI AATT 136 140 . . 147 157 + FalI AAGNNNNNCTT 138 133 170 165 153 156 + SetI ASST 156 152 . . 147 157 - FalI AAGNNNNNCTT 170 165 138 133 181 185 - BsmAI GTCTC 175 179 . . 198 203 + ApoI RAATTY 198 202 . . 199 202 + TspEI AATT 198 202 . . 214 224 + Hin4I GAYNNNNNVTC 205 200 237 232 223 226 + TaqI TCGA 223 225 . . 214 224 - Hin4I GAYNNNNNVTC 237 232 205 200 225 229 + MboII GAAGA 237 236 . . 239 242 + TspEI AATT 238 242 . . 240 245 + VspI ATTAAT 241 243 . . 241 244 + MseI TTAA 241 243 . . 247 252 + MfeI CAATTG 247 251 . . 248 251 + TspEI AATT 247 251 . . 252 256 + FokI GGATG 265 269 . . 252 256 + BseGI GGATG 258 256 . . 254 258 + TspDTI ATGAA 269 267 . . 256 260 + MboII GAAGA 268 267 . . 267 272 + SduI GDGCHC 271 267 . . 267 272 + BseSI GKGCMC 271 267 . . 270 276 + TspRI CASTGNN 276 267 . . 273 276 + CviRI* TGCA 274 274 . . 270 275 - BtsI GCAGTG 269 267 . . 300 305 + SspI AATATT 302 302 . . 311 314 + SetI ASST 314 310 . . 319 324 + BsrDI GCAATG 326 324 . . 322 326 + TspDTI ATGAA 337 335 . . 327 330 + SetI ASST 330 326 . . 333 337 + Hpy188I TCNGA 335 334 . . 339 344 + SspI AATATT 341 341 . . 343 346 + MseI TTAA 343 345 . . 345 350 + AclI AACGTT 346 348 . . 346 349 + TaiI ACGT 349 345 . . 346 349 + SetI ASST 349 345 . . 346 349 + MaeII ACGT 346 348 . . 359 362 + CviJI RGCY 360 360 . . 372 375 + SetI ASST 375 371 . . 378 382 + MboII GAAGA 390 389 . . 378 383 - Ksp632I* CTCTTC 373 376 . . 381 384 - MnlI CCTC 374 373 . . 386 389 + MseI TTAA 386 388 . . 399 409 + Hin4I GAYNNNNNVTC 390 385 422 417 399 403 + HgaI GACGC 408 413 . . 399 404 + AcyI GRCGYC 400 402 . . 406 409 + DpnI GATC 407 407 . . 406 409 + BstKTI GATC 408 406 . . 406 409 + MboI GATC 405 409 . . 408 412 + Hpy188I TCNGA 410 409 . . 399 409 - Hin4I GAYNNNNNVTC 422 417 390 385 415 419 + TauI GCSGC 418 415 . . 415 419 + BlsI GCNGC 417 416 . . 415 419 + BisI GCNGC 416 417 . . 417 420 + CviJI RGCY 418 418 . . 415 418 - AciI CCGC 415 417 . . 422 426 + TspDTI ATGAA 437 435 . . 430 434 - TspDTI ATGAA 420 418 . . 435 440 + Hpy178III* TCNNGA 436 438 . . 488 493 + Hpy166II GTNNAC 490 490 . . 496 500 + MaeIII GTNAC 495 500 . . 505 508 + FatI CATG 504 508 . . 505 508 + NlaIII CATG 508 504 . . 505 508 + CviAII CATG 505 507 . . 503 507 - TspDTI ATGAA 493 491 . . 508 512 + BmgT120I GGNCC 509 510 . . 508 512 + AvaII GGWCC 508 511 . . 508 512 + AsuI* GGNCC 508 511 . . 511 515 + BssKI CCNGG 510 515 . . 511 515 + BseBI CCWGG 512 513 . . 511 515 + ScrFI CCNGG 512 513 . . 511 515 + EcoRII CCWGG 510 515 . . 514 517 + CviJI RGCY 515 515 . . 514 517 + HaeIII GGCC 515 515 . . 519 522 + TspEI AATT 518 522 . . 584 587 + FatI CATG 583 587 . . 584 587 + NlaIII CATG 587 583 . . 584 587 + CviAII CATG 584 586 . . 585 590 + EcoT22I ATGCAT 589 585 . . 582 586 - TspDTI ATGAA 572 570 . . 586 589 + CviRI* TGCA 587 587 . . 594 599 + Cac8I GCNNGC 596 596 . . 597 600 + CviJI RGCY 598 598 . . 597 600 + HaeIII GGCC 598 598 . . 605 609 + Hpy188I TCNGA 607 606 . . 609 614 + AclI AACGTT 610 612 . . 610 613 + TaiI ACGT 613 609 . . 610 613 + SetI ASST 613 609 . . 610 613 + MaeII ACGT 610 612 . . 615 619 + MboII GAAGA 627 626 . . 633 637 + Hpy99I CGWCG 637 632 . . 639 645 + TspRI CASTGNN 645 636 . . 647 650 + CviJI RGCY 648 648 . . 657 661 + DdeI CTNAG 657 660 . . 661 665 - SfaNI GCATC 651 655 . . 681 687 + TspRI CASTGNN 687 678 . . 682 686 + BsrI ACTGG 687 685 . . 683 688 + Eco57MI CTGRAG 704 702 . . 683 688 + GsuI CTGGAG 704 702 . . 685 695 - BsaXI ACNNNNNCTCC 707 704 677 674 708 712 + MboII GAAGA 720 719 . . 719 722 + MaeI CTAG 719 721 . . 724 729 - MmeI TCCRAC 705 703 . . 732 737 + AflIII ACRYGT 732 736 . . 733 738 + BtrI CACGTC 735 735 . . 734 737 + TaiI ACGT 737 733 . . 734 737 + SetI ASST 737 733 . . 734 737 + MaeII ACGT 734 736 . . 737 741 + Hpy188I TCNGA 739 738 . . 741 744 + TaiI ACGT 744 740 . . 741 744 + SetI ASST 744 740 . . 741 744 + MaeII ACGT 741 743 . . 752 757 - BtgZI GCGATG 737 741 . . 768 771 + TspEI AATT 767 771 . . 769 774 + VspI ATTAAT 770 772 . . 770 773 + MseI TTAA 770 772 . . 776 779 + MseI TTAA 776 778 . . 783 788 + XhoII RGATCY 783 787 . . 783 788 + BglII AGATCT 783 787 . . 784 787 + DpnI GATC 785 785 . . 784 787 + BstKTI GATC 786 784 . . 784 787 + MboI GATC 783 787 . . 796 800 + TspDTI ATGAA 811 809 . . 815 818 - AciI CCGC 815 817 . . 830 836 + TspRI CASTGNN 836 827 . . 843 846 + TspEI AATT 842 846 . . 862 866 - SfaNI GCATC 852 856 . . 878 881 + CviRI* TGCA 879 879 . . 891 894 + TspEI AATT 890 894 . . 917 920 + SetI ASST 920 916 . . 917 920 + CviJI RGCY 918 918 . . 917 920 + AluI AGCT 918 918 . . 918 922 + BlsI GCNGC 920 919 . . 918 922 + BisI GCNGC 919 920 . . 918 922 + TseI GCWGC 918 921 . . 922 925 + MaeI CTAG 922 924 . . 918 922 - BbvI GCAGC 905 909 . . 944 949 + BsmI GAATGC 950 948 . . 947 950 + CviRI* TGCA 948 948 . . 951 954 + TspEI AATT 950 954 . . 955 963 + Tth111I GACNNNGTC 958 959 . . 957 961 + Hpy99I CGWCG 961 956 . . 958 963 + ZraI GACGTC 960 960 . . 958 963 + AcyI GRCGYC 959 961 . . 958 963 + AatII GACGTC 962 958 . . 959 962 + TaiI ACGT 962 958 . . 959 962 + SetI ASST 962 958 . . 959 962 + MaeII ACGT 959 961 . . 964 969 + SspI AATATT 966 966 . . 978 982 + TfiI GAWTC 978 981 . . 978 982 + HinfI GANTC 978 981 . . 981 985 - TspGWI ACGGA 971 969 . . #--------------------------------------- # # # AatII: # [GACGTC] # 25 962 # # AciI: # [CCGC] # 172 400 415 # # AclI: # [AACGTT] # 264 346 377 # # AcyI: # [GRCGYC] # 28 400 559 # # AflIII: # [ACRYGT] # 255 732 # # AluI: # [AGCT] # 69 918 # # ApoI: # [RAATTY] # 198 789 # # AsuI*: # [GGNCC] # 479 508 # # AvaII: # [GGWCC] # 479 508 # # BbvCI: # [CCTCAGC] # 111 876 # # BbvI: # [GCAGC] # 82 905 # # Bce83I*: # [CTTGAG] # 67 920 # # BglII: # [AGATCT] # 204 783 # # BisI: # [GCNGC] # 68 416 503 # # BlsI: # [GCNGC] # 67 417 503 # # BmgT120I: # [GGNCC] # 478 509 # # Bpu10I: # [CCTNAGC] # 111 876 # # BsaXI: # [ACNNNNNCTCC] # 30 280 677 # # BseBI: # [CCWGG] # 475 512 # # BseGI: # [GGATG] # 96 162 729 # # BseMII: # [CTCAG] # 13 125 849 # # BseSI: # [GKGCMC] # 271 716 # # BsmAI: # [GTCTC] # 175 812 # # BsmI: # [GAATGC] # 37 950 # # BspCNI: # [CTCAG] # 13 124 850 # # BsrDI: # [GCAATG] # 326 661 # # BsrI: # [ACTGG] # 18 300 669 # # BssKI: # [CCNGG] # 477 510 # # BstKTI: # [GATC] # 201 378 408 # # BtgZI: # [GCGATG] # 250 737 # # BtrI: # [CACGTC] # 252 735 # # BtsI: # [GCAGTG] # 269 718 # # Cac8I: # [GCNNGC] # 391 596 # # CviAII: # [CATG] # 79 403 505 # # CviJI: # [RGCY] # 50 58 69 83 97 270 # 360 # # CviRI*: # [TGCA] # 29 39 69 245 292 313 # # DdeI: # [CTNAG] # 13 111 330 533 # # DpnI: # [GATC] # 202 378 407 # # Eco57MI: # [CTGRAG] # 283 704 # # EcoRII: # [CCWGG] # 477 510 # # EcoT22I: # [ATGCAT] # 398 589 # # FalI: # [AAGNNNNNCTT] # 0 0 32 138 817 # # FatI: # [CATG] # 79 404 504 # # FokI: # [GGATG] # 83 182 722 # # GlaI: # [GCGC] # 116 871 # # GsuI: # [CTGGAG] # 283 704 # # HaeIII: # [GGCC] # 83 389 515 # # HgaI: # [GACGC] # 408 579 # # HhaI: # [GCGC] # 117 870 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 153 205 565 # # Hin6I: # [GCGC] # 115 872 # # HinfI: # [GANTC] # 9 978 # # Hpy166II: # [GTNNAC] # 490 497 # # Hpy178III*: # [TCNNGA] # 436 551 # # Hpy188I: # [TCNGA] # 75 127 132 197 208 248 # # Hpy99I: # [CGWCG] # 26 324 637 # # Ksp632I*: # [CTCTTC] # 373 614 # # MaeI: # [CTAG] # 65 203 719 # # MaeII: # [ACGT] # 7 28 124 218 264 346 # # MaeIII: # [GTNAC] # 492 495 # # MboI: # [GATC] # 204 378 405 # # MboII: # [GAAGA] # 31 93 122 237 237 267 # # MfeI: # [CAATTG] # 247 740 # # MmeI: # [TCCRAC] # 282 705 # # MnlI: # [CCTC] # 36 84 254 613 # # MseI: # [TTAA] # 6 43 102 211 241 384 # # NlaIII: # [CATG] # 79 400 508 # # ScrFI: # [CCNGG] # 475 512 # # SduI: # [GDGCHC] # 271 716 # # SetI: # [ASST] # 7 10 16 19 25 26 # 42 124 146 158 176 238 # # SfaNI: # [GCATC] # 135 201 651 # # SfeI*: # [CTRYAG] # 45 942 # # SmlI: # [CTYRAG] # 82 905 # # SspI: # [AATATT] # 21 39 302 625 # # TaiI: # [ACGT] # 7 25 124 218 264 349 # # TaqI: # [TCGA] # 4 219 764 # # TauI: # [GCSGC] # 418 569 # # TfiI: # [GAWTC] # 9 978 # # TseI: # [GCWGC] # 69 918 # # TspDTI: # [ATGAA] # 17 56 68 79 83 176 # 239 269 # # TspEI: # [AATT] # 9 37 40 48 60 62 # 75 136 249 271 # # TspGWI: # [ACGGA] # 12 16 959 # # TspRI: # [CASTGNN] # 42 149 151 276 369 # # Tth111I: # [GACNNNGTC] # 29 958 # # VspI: # [ATTAAT] # 217 241 529 # # XhoII: # [RGATCY] # 204 783 # # ZraI: # [GACGTC] # 27 960 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 987 # Reported_sequences: 1 # Reported_hitcount: 176 #---------------------------------------