Restriction Fragments for NCW2/YLR194C



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The currently selected gene/sequence is :

NCW2/YLR194C on chromosome XII from coordinates 541573 to 540809.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22161.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 5 10 + StuI AGGCCT 7 7 . . 6 9 + CviJI RGCY 7 7 . . 6 9 + HaeIII GGCC 7 7 . . 28 31 + SetI ASST 31 27 . . 31 34 + MseI TTAA 31 33 . . 33 36 + TspEI AATT 32 36 . . 40 43 + MaeI CTAG 40 42 . . 42 45 + CviJI RGCY 43 43 . . 43 47 + TauI GCSGC 46 43 . . 43 47 + BlsI GCNGC 45 44 . . 43 47 + BisI GCNGC 44 45 . . 44 49 + NspBII* CMGCKG 46 46 . . 44 47 + AciI CCGC 44 46 . . 46 50 + BlsI GCNGC 48 47 . . 46 50 + BisI GCNGC 47 48 . . 46 50 + TseI GCWGC 46 49 . . 46 50 - BbvI GCAGC 33 37 . . 58 62 + HinfI GANTC 58 61 . . 58 62 - PleI GAGTC 52 53 . . 58 62 - MlyI GAGTC 52 52 . . 64 69 + NlaIV GGNNCC 66 66 . . 69 73 + DdeI CTNAG 69 72 . . 75 80 + BalI TGGCCA 77 77 . . 75 80 + CfrI YGGCCR 75 79 . . 76 79 + CviJI RGCY 77 77 . . 76 79 + HaeIII GGCC 77 77 . . 73 77 - BccI CCATC 67 68 . . 85 89 + Hpy188I TCNGA 87 86 . . 86 91 + Eco57MI CTGRAG 107 105 . . 86 91 + Eco57I CTGAAG 107 105 . . 88 92 + MboII GAAGA 100 99 . . 94 97 + SetI ASST 97 93 . . 94 97 + CviJI RGCY 95 95 . . 94 97 + AluI AGCT 95 95 . . 109 112 + SetI ASST 112 108 . . 109 112 + CviJI RGCY 110 110 . . 109 112 + AluI AGCT 110 110 . . 119 124 + SmlI CTYRAG 119 123 . . 120 125 + Hpy178III* TCNNGA 121 123 . . 119 124 - Bce83I* CTTGAG 104 102 . . 126 129 + DpnI GATC 127 127 . . 126 129 + BstKTI GATC 128 126 . . 126 129 + MboI GATC 125 129 . . 132 135 + SetI ASST 135 131 . . 150 153 + CviJI RGCY 151 151 . . 161 164 + Hin6I GCGC 161 163 . . 161 164 + GlaI GCGC 162 162 . . 161 164 + HhaI GCGC 163 161 . . 163 167 + SfaNI GCATC 172 176 . . 169 173 + Tsp4CI* ACNGT 171 170 . . 170 175 + SfeI* CTRYAG 170 174 . . 181 186 + BetI* WCCGGW 181 185 . . 182 185 + HpaII CCGG 182 184 . . 189 194 + HindIII AAGCTT 189 193 . . 190 193 + SetI ASST 193 189 . . 190 193 + CviJI RGCY 191 191 . . 190 193 + AluI AGCT 191 191 . . 192 196 + DdeI CTNAG 192 195 . . 195 200 + TatI WGTACW 195 199 . . 196 199 + Csp6I GTAC 196 198 . . 196 199 + RsaI GTAC 197 197 . . 203 206 + MaeI CTAG 203 205 . . 209 212 + TaiI ACGT 212 208 . . 209 212 + SetI ASST 212 208 . . 209 212 + MaeII ACGT 209 211 . . 210 214 + Hpy99I CGWCG 214 209 . . 223 226 + SetI ASST 226 222 . . 234 237 + CviJI RGCY 235 235 . . 234 239 + NlaIV GGNNCC 236 236 . . 242 245 + Csp6I GTAC 242 244 . . 242 245 + RsaI GTAC 243 243 . . 246 255 + MslI CAYNNNNRTG 250 250 . . 246 255 + OliI CACNNNNGTG 250 250 . . 247 251 + Tsp4CI* ACNGT 249 248 . . 253 257 + Tsp45I GTSAC 252 257 . . 253 257 + MaeIII GTNAC 252 257 . . 257 261 + Hpy99I CGWCG 261 256 . . 259 263 + TspGWI ACGGA 274 272 . . 261 265 + FokI GGATG 274 278 . . 261 265 + BseGI GGATG 267 265 . . 264 267 + CviRI* TGCA 265 265 . . 262 266 - SfaNI GCATC 252 256 . . 285 289 + Tsp4CI* ACNGT 287 286 . . 288 291 + Csp6I GTAC 288 290 . . 288 291 + RsaI GTAC 289 289 . . 290 293 + SetI ASST 293 289 . . 291 301 + BsiYI* CCNNNNNNNGG 297 294 . . 302 307 + CfrI YGGCCR 302 306 . . 298 303 - TaqII CACCCA 288 286 . . 303 306 + CviJI RGCY 304 304 . . 303 306 + HaeIII GGCC 304 304 . . 319 323 - BceAI ACGGC 304 306 . . 324 334 + FalI AAGNNNNNCTT 315 310 347 342 324 334 - FalI AAGNNNNNCTT 347 342 315 310 341 344 + MnlI CCTC 351 350 . . 346 351 + BsgI GTGCAG 367 365 . . 347 350 + CviRI* TGCA 348 348 . . 348 352 + BlsI GCNGC 350 349 . . 348 352 + BisI GCNGC 349 350 . . 348 352 + BbvI GCAGC 360 364 . . 348 352 + TseI GCWGC 348 351 . . 350 353 + CviJI RGCY 351 351 . . 355 360 + BetI* WCCGGW 355 359 . . 356 359 + HpaII CCGG 356 358 . . 363 368 + ZraI GACGTC 365 365 . . 363 368 + AcyI GRCGYC 364 366 . . 363 368 + AatII GACGTC 367 363 . . 364 367 + TaiI ACGT 367 363 . . 364 367 + SetI ASST 367 363 . . 364 367 + MaeII ACGT 364 366 . . 361 365 - BsmAI GTCTC 355 359 . . 361 366 - Esp3I CGTCTC 355 359 . . 374 378 + TfiI GAWTC 374 377 . . 374 378 + HinfI GANTC 374 377 . . 371 374 - AciI CCGC 371 373 . . 377 381 - HphI GGTGA 369 368 . . 386 389 + AciI CCGC 386 388 . . 388 392 + SfaNI GCATC 397 401 . . 392 395 + MnlI CCTC 402 401 . . 389 393 - FokI GGATG 375 379 . . 389 393 - BseGI GGATG 388 386 . . 395 398 + MnlI CCTC 405 404 . . 393 398 - BseRI GAGGAG 384 382 . . 415 419 + TspGWI ACGGA 430 428 . . 421 425 + BmgT120I GGNCC 422 423 . . 421 425 + AsuI* GGNCC 421 424 . . 422 425 + CviJI RGCY 423 423 . . 422 425 + HaeIII GGCC 423 423 . . 427 431 + MaeIII GTNAC 426 431 . . 424 428 - BsrI ACTGG 424 422 . . 431 436 + SfeI* CTRYAG 431 435 . . 436 440 + MaeIII GTNAC 435 440 . . 447 452 + EcoRI GAATTC 447 451 . . 447 452 + ApoI RAATTY 447 451 . . 448 451 + TspEI AATT 447 451 . . 456 459 + SetI ASST 459 455 . . 457 461 + HphI GGTGA 469 468 . . 460 464 - BsmAI GTCTC 454 458 . . 475 479 + Tsp4CI* ACNGT 477 476 . . 485 488 + CviJI RGCY 486 486 . . 484 489 - TsoI TARCCA 474 472 . . 489 493 + Tsp4CI* ACNGT 491 490 . . 494 497 + MnlI CCTC 504 503 . . 494 500 + Bpu10I CCTNAGC 495 498 . . 494 500 + BbvCI CCTCAGC 495 498 . . 495 499 + DdeI CTNAG 495 498 . . 495 499 + BspCNI CTCAG 508 506 . . 495 499 + BseMII CTCAG 509 507 . . 498 501 + CviJI RGCY 499 499 . . 514 518 + BceAI ACGGC 530 532 . . 516 519 + CviJI RGCY 517 517 . . 519 523 - TspDTI ATGAA 509 507 . . 538 543 + Hpy178III* TCNNGA 539 541 . . 535 539 - MboII GAAGA 527 526 . . 527 539 - BarI GAAGNNNNNNTAC 551 546 519 514 541 546 + XhoII RGATCY 541 545 . . 541 546 + NlaIV GGNNCC 543 543 . . 541 546 + BamHI GGATCC 541 545 . . 541 545 + BinI* GGATC 549 550 . . 542 545 + DpnI GATC 543 543 . . 542 545 + BstKTI GATC 544 542 . . 542 551 + BsaBI GATNNNNATC 546 546 . . 542 545 + MboI GATC 541 545 . . 545 548 + MnlI CCTC 555 554 . . 542 546 - BinI* GGATC 536 537 . . 555 560 + BetI* WCCGGW 555 559 . . 556 559 + HpaII CCGG 556 558 . . 559 563 + TfiI GAWTC 559 562 . . 559 563 + HinfI GANTC 559 562 . . 569 574 + StyI CCWWGG 569 573 . . 569 574 + SecI* CCNNGG 569 573 . . 572 575 + SetI ASST 575 571 . . 607 610 + TaiI ACGT 610 606 . . 607 610 + SetI ASST 610 606 . . 607 610 + MaeII ACGT 607 609 . . 612 615 + SetI ASST 615 611 . . 617 622 + SfeI* CTRYAG 617 621 . . 619 623 + Tsp4CI* ACNGT 621 620 . . 629 635 + TspRI CASTGNN 635 626 . . 625 629 - BsmAI GTCTC 619 623 . . 648 653 + Hpy166II GTNNAC 650 650 . . 648 653 + AccI GTMKAC 649 651 . . 650 655 + SfeI* CTRYAG 650 654 . . 652 656 + Tsp4CI* ACNGT 654 653 . . 655 660 + Hpy166II GTNNAC 657 657 . . 655 660 + HindII GTYRAC 657 657 . . 659 663 + Tsp4CI* ACNGT 661 660 . . 665 668 + SetI ASST 668 664 . . 670 674 - HgaI GACGC 659 664 . . 673 677 - TspGWI ACGGA 663 661 . . 685 688 + TspEI AATT 684 688 . . 697 702 + NarI GGCGCC 698 700 . . 697 702 + KasI GGCGCC 697 701 . . 697 702 + DinI GGCGCC 699 699 . . 697 702 + HaeII RGCGCY 701 697 . . 697 702 + NlaIV GGNNCC 699 699 . . 697 702 + AcyI GRCGYC 698 700 . . 697 702 + HgiCI* GGYRCC 697 701 . . 698 701 + Hin6I GCGC 698 700 . . 698 701 + GlaI GCGC 699 699 . . 698 701 + HhaI GCGC 700 698 . . 706 711 + NaeI GCCGGC 708 708 . . 706 711 + MroNI GCCGGC 706 710 . . 706 711 + Cac8I GCNNGC 708 708 . . 706 711 + Cfr10I RCCGGY 706 710 . . 707 710 + HpaII CCGG 707 709 . . 720 723 + SetI ASST 723 719 . . 720 723 + CviJI RGCY 721 721 . . 720 723 + AluI AGCT 721 721 . . 721 731 + MwoI GCNNNNNNNGC 727 724 . . 730 733 - AciI CCGC 730 732 . . 730 734 - FauI CCCGC 723 725 . . 735 738 + CviRI* TGCA 736 736 . . 736 741 - BsmI GAATGC 736 734 . . 742 752 + MwoI GCNNNNNNNGC 748 745 . . 742 746 - BceAI ACGGC 727 729 . . 748 753 + NlaIV GGNNCC 750 750 . . 748 753 + HgiCI* GGYRCC 748 752 . . 749 754 + SduI GDGCHC 753 749 . . 749 754 + BseSI GKGCMC 753 749 . . #--------------------------------------- # # # AatII: # [GACGTC] # 367 398 # # AccI: # [GTMKAC] # 116 649 # # AciI: # [CCGC] # 15 35 44 327 344 # # AcyI: # [GRCGYC] # 67 334 364 # # AluI: # [AGCT] # 15 44 81 95 530 # # ApoI: # [RAATTY] # 318 447 # # AsuI*: # [GGNCC] # 344 421 # # BalI: # [TGGCCA] # 77 688 # # BamHI: # [GGATCC] # 224 541 # # BarI: # [GAAGNNNNNNTAC] # 32 214 519 # # BbvCI: # [CCTCAGC] # 270 495 # # BbvI: # [GCAGC] # 33 327 405 # # BccI: # [CCATC] # 67 698 # # Bce83I*: # [CTTGAG] # 104 661 # # BceAI: # [ACGGC] # 38 197 226 304 # # BetI*: # [WCCGGW] # 174 181 200 210 # # BinI*: # [GGATC] # 13 216 536 # # BisI: # [GCNGC] # 3 44 302 416 # # BlsI: # [GCNGC] # 3 45 302 415 # # BmgT120I: # [GGNCC] # 343 422 # # Bpu10I: # [CCTNAGC] # 270 495 # # BsaBI: # [GATNNNNATC] # 219 546 # # BseGI: # [GGATG] # 121 267 377 # # BseMII: # [CTCAG] # 256 509 # # BseRI: # [GAGGAG] # 381 384 # # BseSI: # [GKGCMC] # 12 753 # # BsgI: # [GTGCAG] # 367 398 # # BsiYI*: # [CCNNNNNNNGG] # 297 468 # # BsmAI: # [GTCTC] # 99 146 165 355 # # BsmI: # [GAATGC] # 29 736 # # BspCNI: # [CTCAG] # 257 508 # # BsrI: # [ACTGG] # 341 424 # # BstKTI: # [GATC] # 128 221 416 # # Cac8I: # [GCNNGC] # 57 708 # # Cfr10I: # [RCCGGY] # 59 706 # # CfrI: # [YGGCCR] # 75 227 463 # # Csp6I: # [GTAC] # 46 46 196 477 # # CviJI: # [RGCY] # 7 13 15 18 18 34 # 36 40 41 44 44 47 # 63 69 72 204 # # CviRI*: # [TGCA] # 29 83 265 388 # # DdeI: # [CTNAG] # 69 123 270 303 # # DinI: # [GGCGCC] # 66 699 # # DpnI: # [GATC] # 127 222 416 # # Eco57I: # [CTGAAG] # 107 658 # # Eco57MI: # [CTGRAG] # 107 658 # # EcoRI: # [GAATTC] # 318 447 # # Esp3I: # [CGTCTC] # 355 410 # # FalI: # [AAGNNNNNCTT] # 0 0 32 315 418 # # FauI: # [CCCGC] # 42 723 # # FokI: # [GGATG] # 101 274 390 # # GlaI: # [GCGC] # 66 162 537 # # HaeII: # [RGCGCY] # 64 701 # # HaeIII: # [GGCC] # 7 70 119 227 342 # # HgaI: # [GACGC] # 106 659 # # HgiCI*: # [GGYRCC] # 17 51 697 # # HhaI: # [GCGC] # 65 163 537 # # Hin6I: # [GCGC] # 67 161 537 # # HindII: # [GTYRAC] # 108 657 # # HindIII: # [AAGCTT] # 189 576 # # HinfI: # [GANTC] # 58 185 206 316 # # HpaII: # [CCGG] # 58 151 174 182 200 # # HphI: # [GGTGA] # 100 296 369 # # Hpy166II: # [GTNNAC] # 7 108 650 # # Hpy178III*: # [TCNNGA] # 121 226 418 # # Hpy188I: # [TCNGA] # 87 678 # # Hpy99I: # [CGWCG] # 47 214 504 # # KasI: # [GGCGCC] # 68 697 # # MaeI: # [CTAG] # 40 163 562 # # MaeII: # [ACGT] # 155 158 209 243 # # MaeIII: # [GTNAC] # 9 174 252 330 # # MboI: # [GATC] # 125 224 416 # # MboII: # [GAAGA] # 100 238 427 # # MlyI: # [GAGTC] # 52 713 # # MnlI: # [CCTC] # 3 51 51 99 210 351 # # MroNI: # [GCCGGC] # 59 706 # # MseI: # [TTAA] # 31 734 # # MslI: # [CAYNNNNRTG] # 250 515 # # MwoI: # [GCNNNNNNNGC] # 17 21 727 # # NaeI: # [GCCGGC] # 57 708 # # NarI: # [GGCGCC] # 67 698 # # NlaIV: # [GGNNCC] # 15 51 66 156 170 307 # # NspBII*: # [CMGCKG] # 46 719 # # OliI: # [CACNNNNGTG] # 250 515 # # PleI: # [GAGTC] # 52 713 # # RsaI: # [GTAC] # 46 46 197 476 # # SduI: # [GDGCHC] # 12 753 # # SecI*: # [CCNNGG] # 196 569 # # SetI: # [ASST] # 5 14 15 19 23 31 # 35 42 53 55 58 66 # 67 74 92 116 # # SfaNI: # [GCATC] # 80 145 172 368 # # SfeI*: # [CTRYAG] # 33 115 170 186 261 # # SmlI: # [CTYRAG] # 119 646 # # StuI: # [AGGCCT] # 7 758 # # StyI: # [CCWWGG] # 196 569 # # TaiI: # [ACGT] # 155 155 212 243 # # TaqII: # [CACCCA] # 288 477 # # TatI: # [WGTACW] # 195 570 # # TauI: # [GCSGC] # 46 719 # # TfiI: # [GAWTC] # 185 206 374 # # TseI: # [GCWGC] # 46 302 417 # # TsoI: # [TARCCA] # 291 474 # # Tsp45I: # [GTSAC] # 252 513 # # Tsp4CI*: # [ACNGT] # 7 14 33 38 78 104 # 130 171 190 # # TspDTI: # [ATGAA] # 16 256 493 # # TspEI: # [AATT] # 32 81 237 415 # # TspGWI: # [ACGGA] # 102 156 233 274 # # TspRI: # [CASTGNN] # 130 635 # # XhoII: # [RGATCY] # 224 541 # # ZraI: # [GACGTC] # 365 400 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 765 # Reported_sequences: 1 # Reported_hitcount: 221 #---------------------------------------