Restriction Fragments for RPL37A/YLR185W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RPL37A/YLR185W on chromosome XII from coordinates 522663 to 523288.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26769.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 34 38 + Tsp4CI* ACNGT 36 35 . . 37 42 + Hpy166II GTNNAC 39 39 . . 41 44 + SetI ASST 44 40 . . 53 58 + McrI* CGRYCG 56 54 . . 55 59 + Tsp4CI* ACNGT 57 56 . . 56 61 + DsaI* CCRYGG 56 60 . . 56 61 + SecI* CCNNGG 56 60 . . 78 82 + Hpy188I TCNGA 80 79 . . 79 84 + Eco57MI CTGRAG 100 98 . . 79 84 + Eco57I CTGAAG 100 98 . . 81 85 + MboII GAAGA 93 92 . . 81 86 - Ksp632I* CTCTTC 76 79 . . 92 96 + Hpy188I TCNGA 94 93 . . 99 104 + NspI RCATGY 103 99 . . 99 104 + Cac8I GCNNGC 101 101 . . 99 104 + SphI GCATGC 103 99 . . 100 103 + FatI CATG 99 103 . . 100 103 + NlaIII CATG 103 99 . . 100 103 + CviAII CATG 100 102 . . 102 105 + CviRI* TGCA 103 103 . . 110 115 + ApoI RAATTY 110 114 . . 111 114 + TspEI AATT 110 114 . . 119 123 + DdeI CTNAG 119 122 . . 128 132 + BsrI ACTGG 133 131 . . 143 147 + BsrI ACTGG 148 146 . . 140 144 - BsmAI GTCTC 134 138 . . 147 152 + Hpy166II GTNNAC 149 149 . . 147 152 + BssNAI GTATAC 149 149 . . 147 152 + AccI GTMKAC 148 150 . . 157 161 + TspDTI ATGAA 172 170 . . 159 163 + MboII GAAGA 171 170 . . 159 164 + BbvII* GAAGAC 166 170 . . 171 176 + Hpy166II GTNNAC 173 173 . . 176 187 + BcgI CGANNNNNNTGC 165 163 199 197 182 187 + NspI RCATGY 186 182 . . 183 192 + MslI CAYNNNNRTG 187 187 . . 183 186 + FatI CATG 182 186 . . 183 186 + NlaIII CATG 186 182 . . 183 186 + CviAII CATG 183 185 . . 184 191 + FspAI RTGCGCAY 187 187 . . 185 190 + MstI* TGCGCA 187 187 . . 186 189 + Hin6I GCGC 186 188 . . 186 189 + GlaI GCGC 187 187 . . 186 189 + HhaI GCGC 188 186 . . 189 192 + FatI CATG 188 192 . . 189 192 + NlaIII CATG 192 188 . . 189 192 + CviAII CATG 189 191 . . 198 203 + ApoI RAATTY 198 202 . . 199 202 + TspEI AATT 198 202 . . 204 208 + Tsp4CI* ACNGT 206 205 . . 212 216 + MboII GAAGA 224 223 . . 217 222 + SspI AATATT 219 219 . . 234 240 + SauI* CCTNAGG 235 238 . . 235 239 + DdeI CTNAG 235 238 . . 235 239 - BspCNI CTCAG 227 225 . . 235 239 - BseMII CTCAG 226 224 . . 237 240 - MnlI CCTC 230 229 . . 245 248 + TaqI TCGA 245 247 . . 251 255 + BinI* GGATC 259 260 . . 252 255 + DpnI GATC 253 253 . . 252 255 + BstKTI GATC 254 252 . . 252 255 + MboI GATC 251 255 . . 256 259 + TspEI AATT 255 259 . . 271 275 + MaeIII GTNAC 270 275 . . 274 278 + Tsp4CI* ACNGT 276 275 . . 277 281 + Tsp45I GTSAC 276 281 . . 277 281 + MaeIII GTNAC 276 281 . . 278 283 + Hpy178III* TCNNGA 279 281 . . 291 295 + MaeIII GTNAC 290 295 . . 310 313 + TspEI AATT 309 313 . . 313 318 + TatI WGTACW 313 317 . . 314 317 + Csp6I GTAC 314 316 . . 314 317 + RsaI GTAC 315 315 . . 326 329 + MseI TTAA 326 328 . . 327 332 + TsoI TARCCA 343 341 . . 333 336 + TspEI AATT 332 336 . . 336 339 + CviRI* TGCA 337 337 . . 344 347 + TspEI AATT 343 347 . . 358 363 + AhaIII* TTTAAA 360 360 . . 359 362 + MseI TTAA 359 361 . . 366 369 - MnlI CCTC 359 358 . . 370 373 + Csp6I GTAC 370 372 . . 370 373 + RsaI GTAC 371 371 . . 375 379 + Hin4II* CCTTC 385 384 . . 375 385 + BsiYI* CCNNNNNNNGG 381 378 . . 377 381 - TspDTI ATGAA 367 365 . . 385 390 + Hpy166II GTNNAC 387 387 . . 389 392 + TaiI ACGT 392 388 . . 389 392 + SetI ASST 392 388 . . 389 392 + MaeII ACGT 389 391 . . 391 395 + Tsp45I GTSAC 390 395 . . 391 395 + MaeIII GTNAC 390 395 . . 393 404 + TstI CACNNNNNNTCC 384 379 416 411 401 405 - BslFI GGGAC 386 390 . . 407 415 + DraIII CACNNNGTG 412 409 . . 415 419 + MaeIII GTNAC 414 419 . . 429 433 + Hpy99I CGWCG 433 428 . . 441 444 + FatI CATG 440 444 . . 441 444 + NlaIII CATG 444 440 . . 441 444 + CviAII CATG 441 443 . . 452 456 + MboII GAAGA 464 463 . . 452 457 + BbvII* GAAGAC 459 463 . . 456 459 + SetI ASST 459 455 . . 463 466 + MnlI CCTC 473 472 . . 470 475 - TsoI TARCCA 460 458 . . 478 483 + PvuII CAGCTG 480 480 . . 478 483 + NspBII* CMGCKG 480 480 . . 479 482 + SetI ASST 482 478 . . 479 482 + CviJI RGCY 480 480 . . 479 482 + AluI AGCT 480 480 . . 480 484 + BlsI GCNGC 482 481 . . 480 484 + BisI GCNGC 481 482 . . 480 484 + TseI GCWGC 480 483 . . 484 488 + DdeI CTNAG 484 487 . . 480 484 - BbvI GCAGC 467 471 . . 492 497 + XhoII RGATCY 492 496 . . 492 497 + BglII AGATCT 492 496 . . 493 496 + DpnI GATC 494 494 . . 493 496 + BstKTI GATC 495 493 . . 493 496 + MboI GATC 492 496 . . 502 506 + BsrI ACTGG 507 505 . . 502 507 + BfiI ACTGGG 512 511 . . 507 512 + NlaIV GGNNCC 509 509 . . 507 512 + HgiCI* GGYRCC 507 511 . . 511 516 + StyI CCWWGG 511 515 . . 511 516 + SecI* CCNNGG 511 515 . . 515 518 + CviJI RGCY 516 516 . . 517 521 + DdeI CTNAG 517 520 . . 523 527 + MboII GAAGA 535 534 . . 523 528 + BbvII* GAAGAC 530 534 . . 523 535 + BarI GAAGNNNNNNTAC 515 510 547 542 534 538 + BsrI ACTGG 539 537 . . 538 541 + Csp6I GTAC 538 540 . . 538 541 + RsaI GTAC 539 539 . . 540 544 + BsrI ACTGG 545 543 . . 563 568 + AflIII ACRYGT 563 567 . . 565 568 + TaiI ACGT 568 564 . . 565 568 + SetI ASST 568 564 . . 565 568 + MaeII ACGT 565 567 . . 570 575 + Hpy178III* TCNNGA 571 573 . . 574 578 + MboII GAAGA 586 585 . . 577 581 + TfiI GAWTC 577 580 . . 577 581 + HinfI GANTC 577 580 . . 580 585 + Hpy178III* TCNNGA 581 583 . . 584 593 + XmnI GAANNNNTTC 588 588 . . 586 590 + Tsp4CI* ACNGT 588 587 . . 597 602 + Cfr10I RCCGGY 597 601 . . 598 601 + HpaII CCGG 598 600 . . 600 603 + CviJI RGCY 601 601 . . 606 616 + MwoI GCNNNNNNNGC 612 609 . . 610 614 + DdeI CTNAG 610 613 . . 614 617 + CviJI RGCY 615 615 . . 623 626 + MseI TTAA 623 625 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 148 478 # # AflIII: # [ACRYGT] # 63 563 # # AhaIII*: # [TTTAAA] # 266 360 # # AluI: # [AGCT] # 146 480 # # ApoI: # [RAATTY] # 88 110 428 # # BarI: # [GAAGNNNNNNTAC] # 32 79 515 # # BbvI: # [GCAGC] # 159 467 # # BbvII*: # [GAAGAC] # 71 96 166 293 # # BcgI: # [CGANNNNNNTGC] # 34 165 427 # # BfiI: # [ACTGGG] # 114 512 # # BglII: # [AGATCT] # 134 492 # # BinI*: # [GGATC] # 259 367 # # BisI: # [GCNGC] # 145 481 # # BlsI: # [GCNGC] # 144 482 # # BseMII: # [CTCAG] # 226 400 # # BsiYI*: # [CCNNNNNNNGG] # 245 381 # # BslFI: # [GGGAC] # 240 386 # # BsmAI: # [GTCTC] # 134 492 # # BspCNI: # [CTCAG] # 227 399 # # BsrI: # [ACTGG] # 6 15 32 81 133 359 # # BssNAI: # [GTATAC] # 149 477 # # BstKTI: # [GATC] # 131 241 254 # # Cac8I: # [GCNNGC] # 101 525 # # Cfr10I: # [RCCGGY] # 29 597 # # Csp6I: # [GTAC] # 56 88 168 314 # # CviAII: # [CATG] # 6 83 100 185 252 # # CviJI: # [RGCY] # 11 14 36 85 480 # # CviRI*: # [TGCA] # 103 234 289 # # DdeI: # [CTNAG] # 16 33 93 116 119 249 # # DpnI: # [GATC] # 132 241 253 # # DraIII: # [CACNNNGTG] # 214 412 # # DsaI*: # [CCRYGG] # 56 570 # # Eco57I: # [CTGAAG] # 100 526 # # Eco57MI: # [CTGRAG] # 100 526 # # FatI: # [CATG] # 6 83 99 186 252 # # FspAI: # [RTGCGCAY] # 187 439 # # GlaI: # [GCGC] # 187 439 # # HgiCI*: # [GGYRCC] # 119 507 # # HhaI: # [GCGC] # 188 438 # # Hin4II*: # [CCTTC] # 241 385 # # Hin6I: # [GCGC] # 186 440 # # HinfI: # [GANTC] # 49 577 # # HpaII: # [CCGG] # 28 598 # # Hpy166II: # [GTNNAC] # 24 39 110 214 239 # # Hpy178III*: # [TCNNGA] # 10 45 279 292 # # Hpy188I: # [TCNGA] # 14 80 532 # # Hpy99I: # [CGWCG] # 193 433 # # Ksp632I*: # [CTCTTC] # 76 550 # # MaeII: # [ACGT] # 61 176 389 # # MaeIII: # [GTNAC] # 6 14 24 100 212 270 # # MboI: # [GATC] # 134 241 251 # # MboII: # [GAAGA] # 40 51 53 71 78 93 # 240 # # McrI*: # [CGRYCG] # 56 570 # # MnlI: # [CCTC] # 114 129 153 230 # # MseI: # [TTAA] # 3 33 264 326 # # MslI: # [CAYNNNNRTG] # 187 439 # # MstI*: # [TGCGCA] # 187 439 # # MwoI: # [GCNNNNNNNGC] # 14 612 # # NlaIII: # [CATG] # 6 83 103 182 252 # # NlaIV: # [GGNNCC] # 117 509 # # NspBII*: # [CMGCKG] # 146 480 # # NspI: # [RCATGY] # 83 103 440 # # PvuII: # [CAGCTG] # 146 480 # # RsaI: # [GTAC] # 56 87 168 315 # # SauI*: # [CCTNAGG] # 235 391 # # SecI*: # [CCNNGG] # 56 115 455 # # SetI: # [ASST] # 23 44 58 67 86 348 # # SphI: # [GCATGC] # 103 523 # # SspI: # [AATATT] # 219 407 # # StyI: # [CCWWGG] # 115 511 # # TaiI: # [ACGT] # 58 176 392 # # TaqI: # [TCGA] # 245 381 # # TatI: # [WGTACW] # 313 313 # # TfiI: # [GAWTC] # 49 577 # # TseI: # [GCWGC] # 146 480 # # TsoI: # [TARCCA] # 117 166 343 # # Tsp45I: # [GTSAC] # 114 236 276 # # Tsp4CI*: # [ACNGT] # 21 36 38 70 149 312 # # TspDTI: # [ATGAA] # 172 195 259 # # TspEI: # [AATT] # 11 23 54 57 88 110 # 283 # # TstI: # [CACNNNNNNTCC] # 32 210 384 # # XhoII: # [RGATCY] # 134 492 # # XmnI: # [GAANNNNTTC] # 38 588 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 626 # Reported_sequences: 1 # Reported_hitcount: 153 #---------------------------------------