Restriction Fragments for TIS11/YLR136C



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The currently selected gene/sequence is :

TIS11/YLR136C on chromosome XII from coordinates 416658 to 415801.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14418.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + SduI GDGCHC 9 5 . . 5 10 + HgiJII* GRGCYC 9 5 . . 6 9 + CviJI RGCY 7 7 . . 11 14 + TspEI AATT 10 14 . . 23 26 + MaeI CTAG 23 25 . . 31 35 + TfiI GAWTC 31 34 . . 31 35 + HinfI GANTC 31 34 . . 35 39 + DdeI CTNAG 35 38 . . 35 39 + BspCNI CTCAG 48 46 . . 35 39 + BseMII CTCAG 49 47 . . 36 40 + Hpy188I TCNGA 38 37 . . 39 43 + MboII GAAGA 51 50 . . 39 44 + BbvII* GAAGAC 46 50 . . 40 50 + FalI AAGNNNNNCTT 31 26 63 58 46 56 + Hin4I GAYNNNNNVTC 37 32 69 64 46 56 + Eam1105I GACNNNNNGTC 51 50 . . 49 52 + MseI TTAA 49 51 . . 40 50 - FalI AAGNNNNNCTT 63 58 31 26 52 55 + TaiI ACGT 55 51 . . 52 55 + SetI ASST 55 51 . . 52 55 + MaeII ACGT 52 54 . . 53 57 + Hpy99I CGWCG 57 52 . . 46 56 - Hin4I GAYNNNNNVTC 69 64 37 32 70 73 + DpnI GATC 71 71 . . 70 73 + BstKTI GATC 72 70 . . 70 73 + MboI GATC 69 73 . . 72 77 + Hpy178III* TCNNGA 73 75 . . 88 91 + MseI TTAA 88 90 . . 102 105 + Csp6I GTAC 102 104 . . 102 105 + RsaI GTAC 103 103 . . 99 103 - BsrI ACTGG 99 97 . . 108 111 + DpnI GATC 109 109 . . 108 111 + BstKTI GATC 110 108 . . 108 111 + MboI GATC 107 111 . . 112 117 + SspI AATATT 114 114 . . 123 127 + BsrI ACTGG 128 126 . . 124 129 + Eco57MI CTGRAG 145 143 . . 124 129 + GsuI CTGGAG 145 143 . . 127 132 + BseRI GAGGAG 142 140 . . 127 130 - MnlI CCTC 120 119 . . 150 155 + AhaIII* TTTAAA 152 152 . . 151 154 + MseI TTAA 151 153 . . 157 160 - MnlI CCTC 150 149 . . 179 184 + BtsI GCAGTG 186 184 . . 180 186 + TspRI CASTGNN 186 177 . . 190 194 - MboII GAAGA 182 181 . . 201 205 - TspDTI ATGAA 191 189 . . 238 242 + BssKI CCNGG 237 242 . . 238 241 + HpaII CCGG 238 240 . . 238 243 + SecI* CCNNGG 238 242 . . 238 242 + ScrFI CCNGG 239 240 . . 238 242 + CauII* CCSGG 239 240 . . 242 245 + CviJI RGCY 243 243 . . 246 250 + Tsp4CI* ACNGT 248 247 . . 253 256 + DpnI GATC 254 254 . . 253 256 + BstKTI GATC 255 253 . . 253 256 + MboI GATC 252 256 . . 253 257 - BinI* GGATC 247 248 . . 258 262 + Tsp4CI* ACNGT 260 259 . . 262 265 + MseI TTAA 262 264 . . 265 269 + TspDTI ATGAA 280 278 . . 280 284 + BccI CCATC 288 289 . . 284 287 + AciI CCGC 284 286 . . 284 289 + BsrBI CCGCTC 286 286 . . 281 285 - FokI GGATG 267 271 . . 281 285 - BseGI GGATG 280 278 . . 290 293 + SetI ASST 293 289 . . 288 292 - HphI GGTGA 280 279 . . 301 305 + BceAI ACGGC 317 319 . . 303 308 + NlaIV GGNNCC 305 305 . . 303 308 + HgiCI* GGYRCC 303 307 . . 306 309 + SetI ASST 309 305 . . 316 321 + AhaIII* TTTAAA 318 318 . . 317 320 + MseI TTAA 317 319 . . 328 331 + MaeI CTAG 328 330 . . 332 335 + SetI ASST 335 331 . . 331 334 - MnlI CCTC 324 323 . . 334 337 + Csp6I GTAC 334 336 . . 334 337 + RsaI GTAC 335 335 . . 345 350 + Hpy178III* TCNNGA 346 348 . . 345 350 + BetI* WCCGGW 345 349 . . 345 350 + BspMII* TCCGGA 345 349 . . 346 349 + HpaII CCGG 346 348 . . 349 357 + Tth111I GACNNNGTC 352 353 . . 355 359 - BslFI GGGAC 340 344 . . 364 368 - MboII GAAGA 356 355 . . 371 374 + TspEI AATT 370 374 . . 379 382 + MseI TTAA 379 381 . . 390 395 + ApoI RAATTY 390 394 . . 391 394 + TspEI AATT 390 394 . . 395 398 + CviRI* TGCA 396 396 . . 399 403 + Tsp4CI* ACNGT 401 400 . . 405 409 + BsmAI GTCTC 410 414 . . 416 419 + CviJI RGCY 417 417 . . 427 431 - SfaNI GCATC 417 421 . . 438 442 - TspDTI ATGAA 428 426 . . 442 446 + Hpy188I TCNGA 444 443 . . 451 455 + TfiI GAWTC 451 454 . . 447 451 - Hin4II* CCTTC 441 440 . . 451 455 + HinfI GANTC 451 454 . . 455 458 + MnlI CCTC 465 464 . . 445 455 - Hin4I GAYNNNNNVTC 468 463 436 431 455 461 + Bpu10I CCTNAGC 456 459 . . 455 461 + BbvCI CCTCAGC 456 459 . . 456 460 + DdeI CTNAG 456 459 . . 456 460 + BspCNI CTCAG 469 467 . . 456 460 + BseMII CTCAG 470 468 . . 459 462 + SetI ASST 462 458 . . 459 462 + CviJI RGCY 460 460 . . 459 462 + AluI AGCT 460 460 . . 465 468 + SetI ASST 468 464 . . 466 472 + SauI* CCTNAGG 467 470 . . 467 471 + DdeI CTNAG 467 470 . . 470 473 + SetI ASST 473 469 . . 471 475 + HphI GGTGA 483 482 . . 478 481 + CviJI RGCY 479 479 . . 480 490 + BsiYI* CCNNNNNNNGG 486 483 . . 484 489 + BsgI GTGCAG 505 503 . . 485 488 + CviRI* TGCA 486 486 . . 503 506 + TspEI AATT 502 506 . . 518 521 + SetI ASST 521 517 . . 518 521 + CviJI RGCY 519 519 . . 518 521 + AluI AGCT 519 519 . . 526 530 + TfiI GAWTC 526 529 . . 526 530 + HinfI GANTC 526 529 . . 537 542 + AhaIII* TTTAAA 539 539 . . 538 541 + MseI TTAA 538 540 . . 546 556 + FalI AAGNNNNNCTT 537 532 569 564 551 561 + MwoI GCNNNNNNNGC 557 554 . . 551 561 + BglI GCCNNNNNGGC 557 554 . . 546 556 - FalI AAGNNNNNCTT 569 564 537 532 581 585 + TspGWI ACGGA 596 594 . . 584 588 + HinfI GANTC 584 587 . . 584 588 - PleI GAGTC 578 579 . . 584 588 - MlyI GAGTC 578 578 . . 586 591 - NmeAIII GCCGAG 566 564 . . 593 596 + TspEI AATT 592 596 . . 595 598 + MseI TTAA 595 597 . . 599 602 + SetI ASST 602 598 . . 602 605 + MseI TTAA 602 604 . . 613 616 + CviRI* TGCA 614 614 . . 618 623 + ApoI RAATTY 618 622 . . 619 622 + TspEI AATT 618 622 . . 623 628 + Hpy178III* TCNNGA 624 626 . . 632 635 + CviJI RGCY 633 633 . . 643 646 + TspEI AATT 642 646 . . 655 658 + MaeI CTAG 655 657 . . 657 660 + SetI ASST 660 656 . . 674 684 + MwoI GCNNNNNNNGC 680 677 . . 674 684 + BstAPI GCANNNNNTGC 680 677 . . 677 681 + MboII GAAGA 689 688 . . 683 687 + BlsI GCNGC 685 684 . . 683 687 + BisI GCNGC 684 685 . . 683 687 + TseI GCWGC 683 686 . . 680 684 - SfaNI GCATC 670 674 . . 683 687 - BbvI GCAGC 670 674 . . 694 697 + FatI CATG 693 697 . . 694 697 + NlaIII CATG 697 693 . . 694 697 + CviAII CATG 694 696 . . 699 703 + Hpy99I CGWCG 703 698 . . 711 716 - BsrDI GCAATG 710 708 . . 718 723 + Hpy166II GTNNAC 720 720 . . 721 726 + BsaAI YACGTR 723 723 . . 722 725 + TaiI ACGT 725 721 . . 722 725 + SetI ASST 725 721 . . 722 725 + MaeII ACGT 722 724 . . 729 732 + SetI ASST 732 728 . . 729 732 + CviJI RGCY 730 730 . . 729 732 + AluI AGCT 730 730 . . 734 737 + Csp6I GTAC 734 736 . . 734 737 + RsaI GTAC 735 735 . . 740 744 + Tsp4CI* ACNGT 742 741 . . 743 747 + MaeIII GTNAC 742 747 . . 746 749 + TaiI ACGT 749 745 . . 746 749 + SetI ASST 749 745 . . 746 749 + MaeII ACGT 746 748 . . 747 752 + Esp3I CGTCTC 753 757 . . 748 752 + BsmAI GTCTC 753 757 . . 750 755 + Ksp632I* CTCTTC 756 759 . . 754 757 + TaqI TCGA 754 756 . . 751 755 - MboII GAAGA 743 742 . . 743 755 - BarI GAAGNNNNNNTAC 767 762 735 730 758 761 + MaeI CTAG 758 760 . . 769 773 + Hpy188I TCNGA 771 770 . . 785 795 + MwoI GCNNNNNNNGC 791 788 . . 785 790 + BsrDI GCAATG 792 790 . . 793 796 + FnuDII* CGCG 794 794 . . 794 798 + TauI GCSGC 797 794 . . 794 798 + BlsI GCNGC 796 795 . . 794 798 + BisI GCNGC 795 796 . . 796 799 + CviJI RGCY 797 797 . . 796 801 + NlaIV GGNNCC 798 798 . . 794 797 - AciI CCGC 794 796 . . 800 803 + AciI CCGC 800 802 . . 799 804 - EciI GGCGGA 789 787 . . 807 811 + MboII GAAGA 819 818 . . 810 814 + MboII GAAGA 822 821 . . 813 817 + TfiI GAWTC 813 816 . . 813 817 + HinfI GANTC 813 816 . . 824 827 + MseI TTAA 824 826 . . 834 837 + CviJI RGCY 835 835 . . 839 842 + CviRI* TGCA 840 840 . . 850 853 + SetI ASST 853 849 . . 850 856 + SexAI ACCWGGT 850 855 . . 851 855 + BssKI CCNGG 850 855 . . 851 855 + BseBI CCWGG 852 853 . . 851 855 + ScrFI CCNGG 852 853 . . 851 855 + EcoRII CCWGG 850 855 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 58 284 510 # # AhaIII*: # [TTTAAA] # 152 166 221 319 # # AluI: # [AGCT] # 59 128 211 460 # # ApoI: # [RAATTY] # 228 240 390 # # BarI: # [GAAGNNNNNNTAC] # 32 91 735 # # BbvCI: # [CCTCAGC] # 402 456 # # BbvI: # [GCAGC] # 188 670 # # BbvII*: # [GAAGAC] # 46 812 # # BccI: # [CCATC] # 288 570 # # BceAI: # [ACGGC] # 317 541 # # BetI*: # [WCCGGW] # 345 513 # # BglI: # [GCCNNNNNGGC] # 301 557 # # BinI*: # [GGATC] # 247 611 # # BisI: # [GCNGC] # 63 111 684 # # BlsI: # [GCNGC] # 62 111 685 # # Bpu10I: # [CCTNAGC] # 402 456 # # BsaAI: # [YACGTR] # 135 723 # # BseBI: # [CCWGG] # 6 852 # # BseGI: # [GGATG] # 280 578 # # BseMII: # [CTCAG] # 49 388 421 # # BseRI: # [GAGGAG] # 142 716 # # BsgI: # [GTGCAG] # 353 505 # # BsiYI*: # [CCNNNNNNNGG] # 372 486 # # BslFI: # [GGGAC] # 340 518 # # BsmAI: # [GTCTC] # 105 343 410 # # BspCNI: # [CTCAG] # 48 389 421 # # BspMII*: # [TCCGGA] # 345 513 # # BsrBI: # [CCGCTC] # 286 572 # # BsrDI: # [GCAATG] # 66 82 710 # # BsrI: # [ACTGG] # 29 99 730 # # BssKI: # [CCNGG] # 8 237 613 # # BstAPI: # [GCANNNNNTGC] # 178 680 # # BstKTI: # [GATC] # 38 72 145 603 # # BtsI: # [GCAGTG] # 186 672 # # CauII*: # [CCSGG] # 239 619 # # Csp6I: # [GTAC] # 102 124 232 400 # # CviAII: # [CATG] # 164 694 # # CviJI: # [RGCY] # 7 19 23 38 40 43 # 67 97 114 174 236 # # CviRI*: # [TGCA] # 18 90 128 226 396 # # DdeI: # [CTNAG] # 11 35 391 421 # # DpnI: # [GATC] # 38 71 145 604 # # Eam1105I: # [GACNNNNNGTC] # 51 807 # # EciI: # [GGCGGA] # 69 789 # # Eco57MI: # [CTGRAG] # 145 713 # # EcoRII: # [CCWGG] # 8 850 # # Esp3I: # [CGTCTC] # 105 753 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 31 32 # 32 289 474 # # FatI: # [CATG] # 165 693 # # FnuDII*: # [CGCG] # 64 794 # # FokI: # [GGATG] # 267 591 # # GsuI: # [CTGGAG] # 145 713 # # HgiCI*: # [GGYRCC] # 303 555 # # HgiJII*: # [GRGCYC] # 9 849 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 37 367 # 390 # # Hin4II*: # [CCTTC] # 417 441 # # HinfI: # [GANTC] # 31 45 58 75 229 420 # # HpaII: # [CCGG] # 108 238 512 # # HphI: # [GGTGA] # 203 280 375 # # Hpy166II: # [GTNNAC] # 138 720 # # Hpy178III*: # [TCNNGA] # 73 234 273 278 # # Hpy188I: # [TCNGA] # 38 87 327 406 # # Hpy99I: # [CGWCG] # 57 155 646 # # Ksp632I*: # [CTCTTC] # 102 756 # # MaeI: # [CTAG] # 23 100 103 305 327 # # MaeII: # [ACGT] # 24 52 112 670 # # MaeIII: # [GTNAC] # 116 742 # # MboI: # [GATC] # 38 69 145 606 # # MboII: # [GAAGA] # 3 36 51 54 76 131 # 174 333 # # MlyI: # [GAGTC] # 280 578 # # MnlI: # [CCTC] # 30 120 141 174 393 # # MseI: # [TTAA] # 7 34 39 49 55 57 # 62 63 111 159 222 # # MwoI: # [GCNNNNNNNGC] # 67 111 123 557 # # NlaIII: # [CATG] # 161 697 # # NlaIV: # [GGNNCC] # 60 305 493 # # NmeAIII: # [GCCGAG] # 292 566 # # PleI: # [GAGTC] # 280 578 # # RsaI: # [GTAC] # 103 123 232 400 # # SauI*: # [CCTNAGG] # 391 467 # # ScrFI: # [CCNGG] # 6 239 613 # # SduI: # [GDGCHC] # 9 849 # # SecI*: # [CCNNGG] # 238 620 # # SetI: # [ASST] # 5 5 6 7 16 17 # 26 48 55 58 65 81 # 104 127 238 # # SexAI: # [ACCWGGT] # 8 850 # # SfaNI: # [GCATC] # 188 253 417 # # SspI: # [AATATT] # 114 744 # # TaiI: # [ACGT] # 24 55 109 670 # # TaqI: # [TCGA] # 104 754 # # TauI: # [GCSGC] # 61 797 # # TfiI: # [GAWTC] # 31 45 75 287 420 # # TseI: # [GCWGC] # 175 683 # # Tsp4CI*: # [ACNGT] # 12 116 141 248 341 # # TspDTI: # [ATGAA] # 89 148 191 430 # # TspEI: # [AATT] # 10 20 24 26 90 112 # 216 360 # # TspGWI: # [ACGGA] # 262 596 # # TspRI: # [CASTGNN] # 186 672 # # Tth111I: # [GACNNNGTC] # 352 506 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 858 # Reported_sequences: 1 # Reported_hitcount: 208 #---------------------------------------