Restriction Fragments for SEN2/YLR105C



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The currently selected gene/sequence is :

SEN2/YLR105C on chromosome XII from coordinates 348180 to 347047.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2745.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 15 - MnlI CCTC 5 4 . . 21 25 + Hpy188I TCNGA 23 22 . . 12 22 - Hin4I GAYNNNNNVTC 35 30 . . 29 33 + MaeIII GTNAC 28 33 . . 36 41 + BciVI GTATCC 47 46 . . 40 43 + MnlI CCTC 50 49 . . 41 46 + Ksp632I* CTCTTC 47 50 . . 42 46 - MboII GAAGA 34 33 . . 48 51 + TspEI AATT 47 51 . . 51 55 - HphI GGTGA 43 42 . . 65 68 + SetI ASST 68 64 . . 66 70 + Hin4II* CCTTC 76 75 . . 69 74 + Hpy178III* TCNNGA 70 72 . . 58 70 - BarI GAAGNNNNNNTAC 82 77 50 45 76 79 + MseI TTAA 76 78 . . 81 85 + Hin4II* CCTTC 91 90 . . 83 87 - TspDTI ATGAA 73 71 . . 90 94 - TspGWI ACGGA 80 78 . . 101 104 + CviJI RGCY 102 102 . . 100 105 - TsoI TARCCA 90 88 . . 117 120 + AciI CCGC 117 119 . . 131 134 + Csp6I GTAC 131 133 . . 131 134 + RsaI GTAC 132 132 . . 140 146 + TspRI CASTGNN 146 137 . . 153 158 + TatI WGTACW 153 157 . . 154 157 + Csp6I GTAC 154 156 . . 154 157 + RsaI GTAC 155 155 . . 156 161 + BspLU11I* ACATGT 156 160 . . 156 161 + NspI RCATGY 160 156 . . 156 161 + AflIII ACRYGT 156 160 . . 157 160 + FatI CATG 156 160 . . 157 160 + NlaIII CATG 160 156 . . 157 160 + CviAII CATG 157 159 . . 182 187 + Ksp632I* CTCTTC 188 191 . . 183 187 - MboII GAAGA 175 174 . . 193 197 + Tsp4CI* ACNGT 195 194 . . 198 203 + Hpy178III* TCNNGA 199 201 . . 246 252 + TspRI CASTGNN 252 243 . . 247 251 + BsrI ACTGG 252 250 . . 254 257 + TspEI AATT 253 257 . . 269 272 + CviJI RGCY 270 270 . . 275 278 + FatI CATG 274 278 . . 275 278 + NlaIII CATG 278 274 . . 275 278 + CviAII CATG 275 277 . . 282 285 + SetI ASST 285 281 . . 282 285 + CviJI RGCY 283 283 . . 282 285 + AluI AGCT 283 283 . . 284 287 + MaeI CTAG 284 286 . . 294 297 + CviJI RGCY 295 295 . . 294 297 + HaeIII GGCC 295 295 . . 292 295 - MnlI CCTC 285 284 . . 299 309 + Hin4I GAYNNNNNVTC 290 285 322 317 299 307 + Tth111I GACNNNGTC 302 303 . . 300 304 + BsrI ACTGG 305 303 . . 300 305 + BfiI ACTGGG 310 309 . . 304 309 + Eco31I GGTCTC 310 314 . . 305 309 + BsmAI GTCTC 310 314 . . 321 324 + MnlI CCTC 331 330 . . 324 334 + BsiYI* CCNNNNNNNGG 330 327 . . 335 338 - MnlI CCTC 328 327 . . 358 362 + TspGWI ACGGA 373 371 . . 377 380 + SetI ASST 380 376 . . 379 383 + Tsp45I GTSAC 378 383 . . 379 383 + MaeIII GTNAC 378 383 . . 375 379 - Hin4II* CCTTC 369 368 . . 390 395 + BseRI GAGGAG 405 403 . . 390 393 - MnlI CCTC 383 382 . . 413 418 + Hpy178III* TCNNGA 414 416 . . 423 426 + TaiI ACGT 426 422 . . 423 426 + SetI ASST 426 422 . . 423 426 + MaeII ACGT 423 425 . . 443 446 + TspEI AATT 442 446 . . 448 451 + MaeI CTAG 448 450 . . 452 455 + TspEI AATT 451 455 . . 454 457 + MseI TTAA 454 456 . . 465 468 + SetI ASST 468 464 . . 472 481 + MslI CAYNNNNRTG 476 476 . . 475 480 + ClaI ATCGAT 476 478 . . 476 479 + TaqI TCGA 476 478 . . 481 484 - MnlI CCTC 474 473 . . 492 495 + MnlI CCTC 502 501 . . 489 493 - FokI GGATG 475 479 . . 489 493 - BseGI GGATG 488 486 . . 496 501 + SmlI CTYRAG 496 500 . . 496 501 + Bce83I* CTTGAG 517 515 . . 511 515 + TfiI GAWTC 511 514 . . 511 515 + HinfI GANTC 511 514 . . 517 520 + MseI TTAA 517 519 . . 523 528 + ApoI RAATTY 523 527 . . 524 527 + TspEI AATT 523 527 . . 526 531 + AhaIII* TTTAAA 528 528 . . 527 530 + MseI TTAA 527 529 . . 544 548 + MboII GAAGA 556 555 . . 544 549 + BbvII* GAAGAC 551 555 . . 548 551 + TaiI ACGT 551 547 . . 548 551 + SetI ASST 551 547 . . 548 551 + MaeII ACGT 548 550 . . 547 552 - BtrI CACGTC 549 549 . . 554 558 + SfaNI GCATC 563 567 . . 561 564 + CviRI* TGCA 562 562 . . 580 584 + Hpy188I TCNGA 582 581 . . 583 587 + TfiI GAWTC 583 586 . . 583 587 + HinfI GANTC 583 586 . . 588 593 + ApoI RAATTY 588 592 . . 589 592 + TspEI AATT 588 592 . . 592 595 + MseI TTAA 592 594 . . 599 603 + TspDTI ATGAA 614 612 . . 601 605 + MboII GAAGA 613 612 . . 601 606 + BbvII* GAAGAC 608 612 . . 615 620 + Hpy178III* TCNNGA 616 618 . . 612 616 - MboII GAAGA 604 603 . . 626 630 + Tsp4CI* ACNGT 628 627 . . 628 632 + HphI GGTGA 640 639 . . 631 634 + DpnI GATC 632 632 . . 631 634 + BstKTI GATC 633 631 . . 631 634 + MboI GATC 630 634 . . 643 646 + MaeI CTAG 643 645 . . 646 650 + PleI GAGTC 654 655 . . 646 650 + MlyI GAGTC 655 655 . . 646 650 + HinfI GANTC 646 649 . . 648 652 + Tsp45I GTSAC 647 652 . . 648 652 + MaeIII GTNAC 647 652 . . 652 655 + MaeI CTAG 652 654 . . 665 671 + TspRI CASTGNN 671 662 . . 664 668 - BsrI ACTGG 664 662 . . 672 675 + SetI ASST 675 671 . . 672 675 + CviJI RGCY 673 673 . . 672 675 + AluI AGCT 673 673 . . 682 685 + MseI TTAA 682 684 . . 690 693 + CviRI* TGCA 691 691 . . 702 707 + Hpy178III* TCNNGA 703 705 . . 715 719 + FauI CCCGC 723 725 . . 716 719 + AciI CCGC 716 718 . . 717 720 + FnuDII* CGCG 718 718 . . 718 728 + MwoI GCNNNNNNNGC 724 721 . . 718 723 + Cac8I GCNNGC 720 720 . . 723 727 + BssKI CCNGG 722 727 . . 723 727 + BseBI CCWGG 724 725 . . 723 727 + ScrFI CCNGG 724 725 . . 723 727 + EcoRII CCWGG 722 727 . . 735 739 + Tsp4CI* ACNGT 737 736 . . 743 746 + TspEI AATT 742 746 . . 746 749 + TaqI TCGA 746 748 . . 748 752 - SfaNI GCATC 738 742 . . 763 773 + Hin4I GAYNNNNNVTC 754 749 786 781 779 783 + Hpy188I TCNGA 781 780 . . 782 785 + DpnI GATC 783 783 . . 782 785 + BstKTI GATC 784 782 . . 782 785 + MboI GATC 781 785 . . 788 791 + TaiI ACGT 791 787 . . 788 791 + SetI ASST 791 787 . . 788 791 + MaeII ACGT 788 790 . . 798 802 + BccI CCATC 806 807 . . 804 809 + SfeI* CTRYAG 804 808 . . 809 812 + DpnI GATC 810 810 . . 809 812 + BstKTI GATC 811 809 . . 809 812 + MboI GATC 808 812 . . 815 819 + Tsp4CI* ACNGT 817 816 . . 829 834 + XhoII RGATCY 829 833 . . 829 834 + BglII AGATCT 829 833 . . 830 833 + DpnI GATC 831 831 . . 830 833 + BstKTI GATC 832 830 . . 830 833 + MboI GATC 829 833 . . 841 846 + ApoI RAATTY 841 845 . . 842 845 + TspEI AATT 841 845 . . 847 850 + CviJI RGCY 848 848 . . 848 852 + BlsI GCNGC 850 849 . . 848 852 + BisI GCNGC 849 850 . . 848 852 + TseI GCWGC 848 851 . . 848 852 - BbvI GCAGC 835 839 . . 874 878 + BmgT120I GGNCC 875 876 . . 874 878 + AsuI* GGNCC 874 877 . . 872 875 - MnlI CCTC 865 864 . . 875 878 + CviJI RGCY 876 876 . . 875 878 + HaeIII GGCC 876 876 . . 895 900 + ApoI RAATTY 895 899 . . 896 899 + TspEI AATT 895 899 . . 912 917 + Hpy178III* TCNNGA 913 915 . . 916 927 + DrdI GACNNNNNNGTC 922 920 . . 921 925 + Hpy99I CGWCG 925 920 . . 922 927 + ZraI GACGTC 924 924 . . 922 927 + AcyI GRCGYC 923 925 . . 922 927 + AatII GACGTC 926 922 . . 923 926 + TaiI ACGT 926 922 . . 923 926 + SetI ASST 926 922 . . 923 926 + MaeII ACGT 923 925 . . 924 929 + Esp3I CGTCTC 930 934 . . 925 929 + BsmAI GTCTC 930 934 . . 939 944 + BsaAI YACGTR 941 941 . . 940 943 + TaiI ACGT 943 939 . . 940 943 + SetI ASST 943 939 . . 940 943 + MaeII ACGT 940 942 . . 950 953 + MaeI CTAG 950 952 . . 957 960 + CviJI RGCY 958 958 . . 960 970 + BsiYI* CCNNNNNNNGG 966 963 . . 971 975 + TauI GCSGC 974 971 . . 971 975 + BlsI GCNGC 973 972 . . 971 975 + BisI GCNGC 972 973 . . 974 977 + Hin6I GCGC 974 976 . . 974 977 + GlaI GCGC 975 975 . . 974 977 + HhaI GCGC 976 974 . . 971 974 - AciI CCGC 971 973 . . 981 985 + MboII GAAGA 993 992 . . 981 986 + BbvII* GAAGAC 988 992 . . 985 988 + TaiI ACGT 988 984 . . 985 988 + SetI ASST 988 984 . . 985 988 + MaeII ACGT 985 987 . . 1000 1005 + BsaAI YACGTR 1002 1002 . . 1001 1004 + TaiI ACGT 1004 1000 . . 1001 1004 + SetI ASST 1004 1000 . . 1001 1004 + MaeII ACGT 1001 1003 . . 1008 1012 - BsmAI GTCTC 1002 1006 . . 1018 1022 + Hpy188I TCNGA 1020 1019 . . 1027 1030 - MnlI CCTC 1020 1019 . . 1064 1067 + TspEI AATT 1063 1067 . . 1087 1091 + HphI GGTGA 1099 1098 . . 1110 1115 + NlaIV GGNNCC 1112 1112 . . 1106 1110 - BccI CCATC 1100 1101 . . 1113 1119 + PfoI TCCNGGA 1113 1118 . . 1114 1118 + BssKI CCNGG 1113 1118 . . 1114 1118 + ScrFI CCNGG 1115 1116 . . 1114 1118 + CauII* CCSGG 1115 1116 . . 1115 1118 + HpaII CCGG 1115 1117 . . 1118 1122 + MboII GAAGA 1130 1129 . . 1131 1134 + MaeI CTAG 1131 1133 . . 1127 1131 - BsmAI GTCTC 1121 1125 . . #--------------------------------------- # # # AatII: # [GACGTC] # 208 926 # # AciI: # [CCGC] # 117 163 255 599 # # AcyI: # [GRCGYC] # 211 923 # # AflIII: # [ACRYGT] # 156 978 # # AhaIII*: # [TTTAAA] # 528 606 # # AluI: # [AGCT] # 283 390 461 # # ApoI: # [RAATTY] # 54 65 239 253 523 # # AsuI*: # [GGNCC] # 260 874 # # BarI: # [GAAGNNNNNNTAC] # 32 50 1052 # # BbvI: # [GCAGC] # 299 835 # # BbvII*: # [GAAGAC] # 57 146 380 551 # # BccI: # [CCATC] # 34 294 806 # # Bce83I*: # [CTTGAG] # 517 617 # # BciVI: # [GTATCC] # 47 1087 # # BfiI: # [ACTGGG] # 310 824 # # BglII: # [AGATCT] # 305 829 # # BisI: # [GCNGC] # 123 162 849 # # BlsI: # [GCNGC] # 123 161 850 # # BmgT120I: # [GGNCC] # 259 875 # # BsaAI: # [YACGTR] # 61 132 941 # # BseBI: # [CCWGG] # 410 724 # # BseGI: # [GGATG] # 488 646 # # BseRI: # [GAGGAG] # 405 729 # # BsiYI*: # [CCNNNNNNNGG] # 168 330 636 # # BsmAI: # [GTCTC] # 13 72 119 310 620 # # BspLU11I*: # [ACATGT] # 156 978 # # BsrI: # [ACTGG] # 53 252 359 470 # # BssKI: # [CCNGG] # 21 391 722 # # BstKTI: # [GATC] # 21 27 151 302 633 # # BtrI: # [CACGTC] # 549 585 # # Cac8I: # [GCNNGC] # 414 720 # # CauII*: # [CCSGG] # 19 1115 # # ClaI: # [ATCGAT] # 476 658 # # Csp6I: # [GTAC] # 23 131 980 # # CviAII: # [CATG] # 118 157 859 # # CviJI: # [RGCY] # 12 13 28 82 102 168 # 175 176 378 # # CviRI*: # [TGCA] # 129 443 562 # # DpnI: # [GATC] # 21 27 151 303 632 # # DrdI: # [GACNNNNNNGTC] # 212 922 # # Eco31I: # [GGTCTC] # 310 824 # # EcoRII: # [CCWGG] # 412 722 # # Esp3I: # [CGTCTC] # 204 930 # # FatI: # [CATG] # 118 156 860 # # FauI: # [CCCGC] # 411 723 # # FnuDII*: # [CGCG] # 416 718 # # FokI: # [GGATG] # 475 659 # # GlaI: # [GCGC] # 159 975 # # HaeIII: # [GGCC] # 258 295 581 # # HhaI: # [GCGC] # 158 976 # # Hin4I: # [GAYNNNNNVTC] # 32 32 35 255 348 432 # # Hin4II*: # [CCTTC] # 15 76 278 765 # # Hin6I: # [GCGC] # 160 974 # # HinfI: # [GANTC] # 63 72 488 511 # # HpaII: # [CCGG] # 19 1115 # # HphI: # [GGTGA] # 35 43 459 597 # # Hpy178III*: # [TCNNGA] # 70 87 129 202 210 215 # 221 # # Hpy188I: # [TCNGA] # 23 114 199 239 559 # # Hpy99I: # [CGWCG] # 209 925 # # Ksp632I*: # [CTCTTC] # 47 141 946 # # MaeI: # [CTAG] # 3 9 164 181 195 284 # 298 # # MaeII: # [ACGT] # 16 17 45 125 133 135 # 240 423 # # MaeIII: # [GTNAC] # 28 269 350 487 # # MboI: # [GATC] # 21 27 151 305 630 # # MboII: # [GAAGA] # 4 9 34 48 137 141 # 380 381 # # MlyI: # [GAGTC] # 479 655 # # MnlI: # [CCTC] # 3 5 28 43 45 52 # 91 114 155 235 363 # # MseI: # [TTAA] # 10 63 65 76 90 378 # 452 # # MslI: # [CAYNNNNRTG] # 476 658 # # MwoI: # [GCNNNNNNNGC] # 410 724 # # NlaIII: # [CATG] # 118 160 856 # # NlaIV: # [GGNNCC] # 22 1112 # # NspI: # [RCATGY] # 160 974 # # PfoI: # [TCCNGGA] # 21 1113 # # PleI: # [GAGTC] # 480 654 # # RsaI: # [GTAC] # 23 132 979 # # ScrFI: # [CCNGG] # 19 391 724 # # SetI: # [ASST] # 16 17 42 45 46 68 # 83 95 116 124 130 135 # 217 # # SfaNI: # [GCATC] # 175 396 563 # # SfeI*: # [CTRYAG] # 330 804 # # SmlI: # [CTYRAG] # 496 638 # # TaiI: # [ACGT] # 16 17 45 125 130 135 # 240 426 # # TaqI: # [TCGA] # 270 388 476 # # TatI: # [WGTACW] # 153 981 # # TauI: # [GCSGC] # 160 974 # # TfiI: # [GAWTC] # 72 511 551 # # TseI: # [GCWGC] # 286 848 # # TsoI: # [TARCCA] # 90 1044 # # Tsp45I: # [GTSAC] # 269 378 487 # # Tsp4CI*: # [ACNGT] # 80 109 195 317 433 # # TspDTI: # [ATGAA] # 73 520 541 # # TspEI: # [AATT] # 9 47 54 65 71 72 # 99 154 168 189 206 # # TspGWI: # [ACGGA] # 80 293 761 # # TspRI: # [CASTGNN] # 106 146 419 463 # # Tth111I: # [GACNNNGTC] # 302 832 # # XhoII: # [RGATCY] # 305 829 # # ZraI: # [GACGTC] # 210 924 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1134 # Reported_sequences: 1 # Reported_hitcount: 226 #---------------------------------------