Restriction Fragments for ALT1/YLR089C



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The currently selected gene/sequence is :

ALT1/YLR089C on chromosome XII from coordinates 320015 to 318237.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28282.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 14 + TspRI CASTGNN 14 5 . . 9 13 + Tsp4CI* ACNGT 11 10 . . 17 30 - AjuI GAANNNNNNNTTGG 42 37 10 5 31 35 + Tsp4CI* ACNGT 33 32 . . 32 38 + TspRI CASTGNN 38 29 . . 40 43 + TspEI AATT 39 43 . . 53 56 + TaiI ACGT 56 52 . . 53 56 + SetI ASST 56 52 . . 53 56 + MaeII ACGT 53 55 . . 59 62 + MseI TTAA 59 61 . . 78 82 + HinfI GANTC 78 81 . . 78 82 - PleI GAGTC 72 73 . . 78 82 - MlyI GAGTC 72 72 . . 92 95 + Hin6I GCGC 92 94 . . 92 95 + GlaI GCGC 93 93 . . 92 95 + HhaI GCGC 94 92 . . 130 133 + SetI ASST 133 129 . . 131 134 + MnlI CCTC 141 140 . . 131 137 + Bpu10I CCTNAGC 132 135 . . 131 137 + BbvCI CCTCAGC 132 135 . . 132 136 + DdeI CTNAG 132 135 . . 132 136 + BspCNI CTCAG 145 143 . . 132 136 + BseMII CTCAG 146 144 . . 135 138 + CviJI RGCY 136 136 . . 137 141 - BsrI ACTGG 137 135 . . 142 147 + TatI WGTACW 142 146 . . 143 146 + Csp6I GTAC 143 145 . . 143 146 + RsaI GTAC 144 144 . . 151 154 + MseI TTAA 151 153 . . 155 160 + HindIII AAGCTT 155 159 . . 156 159 + SetI ASST 159 155 . . 156 159 + CviJI RGCY 157 157 . . 156 159 + AluI AGCT 157 157 . . 161 166 + Hpy178III* TCNNGA 162 164 . . 181 186 + SfeI* CTRYAG 181 185 . . 186 190 - BsmAI GTCTC 180 184 . . 193 196 + SetI ASST 196 192 . . 193 196 + CviJI RGCY 194 194 . . 193 196 + AluI AGCT 194 194 . . 194 200 + SapI GCTCTTC 201 204 . . 195 200 + Ksp632I* CTCTTC 201 204 . . 199 205 + PfoI TCCNGGA 199 204 . . 196 200 - MboII GAAGA 188 187 . . 200 204 + BssKI CCNGG 199 204 . . 200 204 + BseBI CCWGG 201 202 . . 200 204 + ScrFI CCNGG 201 202 . . 200 204 + EcoRII CCWGG 199 204 . . 204 214 + Hin4I GAYNNNNNVTC 195 190 227 222 225 228 + TaiI ACGT 228 224 . . 225 228 + SetI ASST 228 224 . . 225 228 + MaeII ACGT 225 227 . . 228 232 - TspDTI ATGAA 218 216 . . 244 248 - MboII GAAGA 236 235 . . 256 265 + PshAI GACNNNNGTC 260 260 . . 257 260 + SetI ASST 260 256 . . 257 262 + BspMI ACCTGC 266 270 . . 261 265 - HgaI GACGC 250 255 . . 270 274 + TfiI GAWTC 270 273 . . 270 274 + HinfI GANTC 270 273 . . 287 292 + AflIII ACRYGT 287 291 . . 289 292 + TaiI ACGT 292 288 . . 289 292 + SetI ASST 292 288 . . 289 292 + MaeII ACGT 289 291 . . 300 305 + AsuII TTCGAA 301 303 . . 301 304 + TaqI TCGA 301 303 . . 319 323 + FauI CCCGC 327 329 . . 320 323 + AciI CCGC 320 322 . . 328 333 + MfeI CAATTG 328 332 . . 329 332 + TspEI AATT 328 332 . . 340 344 + MboII GAAGA 352 351 . . 340 345 + BbvII* GAAGAC 347 351 . . 344 347 + TaiI ACGT 347 343 . . 344 347 + SetI ASST 347 343 . . 344 347 + MaeII ACGT 344 346 . . 350 354 + TspDTI ATGAA 365 363 . . 359 364 + Hpy178III* TCNNGA 360 362 . . 363 375 + BarI GAAGNNNNNNTAC 355 350 387 382 366 369 + CviJI RGCY 367 367 . . 363 367 - Hin4II* CCTTC 357 356 . . 367 377 + MwoI GCNNNNNNNGC 373 370 . . 368 372 + DdeI CTNAG 368 371 . . 372 375 + Csp6I GTAC 372 374 . . 372 375 + RsaI GTAC 373 373 . . 376 380 - BceAI ACGGC 361 363 . . 384 387 + SetI ASST 387 383 . . 385 390 + NlaIV GGNNCC 387 387 . . 385 390 + HgiCI* GGYRCC 385 389 . . 383 386 - MnlI CCTC 376 375 . . 389 393 + BccI CCATC 397 398 . . 390 399 + MslI CAYNNNNRTG 394 394 . . 383 393 - Hin4I GAYNNNNNVTC 406 401 374 369 390 394 - FokI GGATG 376 380 . . 390 394 - BseGI GGATG 389 387 . . 402 405 + SetI ASST 405 401 . . 402 405 + CviJI RGCY 403 403 . . 402 405 + AluI AGCT 403 403 . . 404 409 + Eco57MI CTGRAG 425 423 . . 404 409 + Eco57I CTGAAG 425 423 . . 406 410 + MboII GAAGA 418 417 . . 410 413 + TspEI AATT 409 413 . . 417 420 + SetI ASST 420 416 . . 417 420 + CviJI RGCY 418 418 . . 417 420 + AluI AGCT 418 418 . . 423 427 + BsrI ACTGG 428 426 . . 424 429 + Eco57MI CTGRAG 445 443 . . 424 429 + GsuI CTGGAG 445 443 . . 432 437 + XhoII RGATCY 432 436 . . 432 437 + NlaIV GGNNCC 434 434 . . 432 437 + BamHI GGATCC 432 436 . . 432 436 + BinI* GGATC 440 441 . . 429 433 - Hin4II* CCTTC 423 422 . . 433 443 + Hin4I GAYNNNNNVTC 424 419 456 451 433 436 + DpnI GATC 434 434 . . 433 436 + BstKTI GATC 435 433 . . 433 436 + MboI GATC 432 436 . . 436 439 + MnlI CCTC 446 445 . . 433 437 - BinI* GGATC 427 428 . . 433 443 - Hin4I GAYNNNNNVTC 456 451 424 419 472 477 + SspI AATATT 474 474 . . 484 487 + MnlI CCTC 494 493 . . 484 490 + Bpu10I CCTNAGC 485 488 . . 484 490 + BbvCI CCTCAGC 485 488 . . 485 489 + DdeI CTNAG 485 488 . . 485 489 + BspCNI CTCAG 498 496 . . 485 489 + BseMII CTCAG 499 497 . . 504 507 + SetI ASST 507 503 . . 505 508 + MnlI CCTC 515 514 . . 507 511 + Hpy188I TCNGA 509 508 . . 516 521 + SfeI* CTRYAG 516 520 . . 524 527 + SetI ASST 527 523 . . 531 535 + BsmAI GTCTC 536 540 . . 556 559 + MseI TTAA 556 558 . . 571 576 + EcoP15I CAGCAG 601 603 . . 573 577 + BlsI GCNGC 575 574 . . 573 577 + BisI GCNGC 574 575 . . 573 577 + BbvI GCAGC 585 589 . . 573 577 + TseI GCWGC 573 576 . . 575 578 + SetI ASST 578 574 . . 575 578 + CviJI RGCY 576 576 . . 575 578 + AluI AGCT 576 576 . . 577 580 + MaeI CTAG 577 579 . . 583 587 + TfiI GAWTC 583 586 . . 583 587 + HinfI GANTC 583 586 . . 585 590 + AsuII TTCGAA 586 588 . . 586 589 + TaqI TCGA 586 588 . . 590 593 + TspEI AATT 589 593 . . 594 601 + PmeI GTTTAAAC 597 597 . . 595 600 + AhaIII* TTTAAA 597 597 . . 596 599 + MseI TTAA 596 598 . . 601 604 + MaeI CTAG 601 603 . . 604 608 - SfaNI GCATC 594 598 . . 611 614 + MseI TTAA 611 613 . . 615 618 + TaiI ACGT 618 614 . . 615 618 + SetI ASST 618 614 . . 615 618 + MaeII ACGT 615 617 . . 618 621 + CviRI* TGCA 619 619 . . 628 631 + MseI TTAA 628 630 . . 634 638 + MboII GAAGA 646 645 . . 637 642 + EcoRV GATATC 639 639 . . 663 668 + Ksp632I* CTCTTC 669 672 . . 664 668 - MboII GAAGA 656 655 . . 671 676 + SmlI CTYRAG 671 675 . . 667 671 - MboII GAAGA 659 658 . . 675 678 + SetI ASST 678 674 . . 671 676 - Bce83I* CTTGAG 656 654 . . 684 687 + SetI ASST 687 683 . . 682 686 - Hin4II* CCTTC 676 675 . . 706 711 + ApoI RAATTY 706 710 . . 707 710 + TspEI AATT 706 710 . . 710 715 + PsiI TTATAA 712 712 . . 717 721 + MboII GAAGA 729 728 . . 717 722 - Ksp632I* CTCTTC 712 715 . . 722 726 + BslFI GGGAC 736 740 . . 720 723 - MnlI CCTC 713 712 . . 727 731 - Hin4II* CCTTC 721 720 . . 735 740 + EcoRV GATATC 737 737 . . 748 751 - MnlI CCTC 741 740 . . 764 769 - EcoP15I CAGCAG 736 738 . . 771 774 + CviRI* TGCA 772 772 . . 772 776 + SfaNI GCATC 781 785 . . 772 782 + MwoI GCNNNNNNNGC 778 775 . . 776 779 + AciI CCGC 776 778 . . 773 777 - FokI GGATG 759 763 . . 773 777 - BseGI GGATG 772 770 . . 778 782 + BlsI GCNGC 780 779 . . 778 782 + BisI GCNGC 779 780 . . 778 782 + BbvI GCAGC 790 794 . . 778 782 + TseI GCWGC 778 781 . . 779 784 + PvuII CAGCTG 781 781 . . 779 784 + NspBII* CMGCKG 781 781 . . 780 783 + SetI ASST 783 779 . . 780 783 + CviJI RGCY 781 781 . . 780 783 + AluI AGCT 781 781 . . 787 790 + TspEI AATT 786 790 . . 801 804 + TspEI AATT 800 804 . . 807 812 + SfeI* CTRYAG 807 811 . . 814 818 + BmgT120I GGNCC 815 816 . . 814 818 + AsuI* GGNCC 814 817 . . 812 815 - MnlI CCTC 805 804 . . 815 818 + CviJI RGCY 816 816 . . 815 818 + HaeIII GGCC 816 816 . . 817 827 + BsiYI* CCNNNNNNNGG 823 820 . . 830 835 + Hpy178III* TCNNGA 831 833 . . 834 838 + TfiI GAWTC 834 837 . . 834 838 + HinfI GANTC 834 837 . . 840 843 + TspEI AATT 839 843 . . 844 847 + MnlI CCTC 854 853 . . 863 866 + AciI CCGC 863 865 . . 865 875 + MwoI GCNNNNNNNGC 871 868 . . 871 874 + MaeI CTAG 871 873 . . 873 876 + SetI ASST 876 872 . . 873 876 + CviJI RGCY 874 874 . . 873 876 + AluI AGCT 874 874 . . 883 886 + TspEI AATT 882 886 . . 887 892 + SmlI CTYRAG 887 891 . . 890 895 + HindIII AAGCTT 890 894 . . 891 894 + SetI ASST 894 890 . . 891 894 + CviJI RGCY 892 892 . . 891 894 + AluI AGCT 892 892 . . 887 892 - Bce83I* CTTGAG 872 870 . . 915 920 + EcoRI GAATTC 915 919 . . 915 920 + ApoI RAATTY 915 919 . . 916 919 + TspEI AATT 915 919 . . 921 924 + SetI ASST 924 920 . . 927 932 + Hpy166II GTNNAC 929 929 . . 927 932 + HindII GTYRAC 929 929 . . 936 941 + Hpy178III* TCNNGA 937 939 . . 940 944 + MboII GAAGA 952 951 . . 945 948 + TspEI AATT 944 948 . . 952 956 + Tsp4CI* ACNGT 954 953 . . 966 969 + CviJI RGCY 967 967 . . 964 967 - MnlI CCTC 957 956 . . 987 990 + SetI ASST 990 986 . . 989 994 + SfeI* CTRYAG 989 993 . . 991 995 + Tsp4CI* ACNGT 993 992 . . 997 1000 + MaeI CTAG 997 999 . . 1001 1006 - TsoI TARCCA 991 989 . . 1012 1016 + BssKI CCNGG 1011 1016 . . 1012 1016 + BseBI CCWGG 1013 1014 . . 1012 1016 + ScrFI CCNGG 1013 1014 . . 1012 1016 + EcoRII CCWGG 1011 1016 . . 1014 1017 + SetI ASST 1017 1013 . . 1022 1027 + SfeI* CTRYAG 1022 1026 . . 1025 1033 + AlwNI CAGNNNCTG 1030 1027 . . 1029 1032 + SetI ASST 1032 1028 . . 1029 1032 + CviJI RGCY 1030 1030 . . 1029 1032 + AluI AGCT 1030 1030 . . 1041 1044 + SetI ASST 1044 1040 . . 1043 1047 + DdeI CTNAG 1043 1046 . . 1039 1043 - HphI GGTGA 1031 1030 . . 1045 1049 + PleI GAGTC 1053 1054 . . 1045 1049 + MlyI GAGTC 1054 1054 . . 1045 1049 + HinfI GANTC 1045 1048 . . 1043 1047 - BspCNI CTCAG 1035 1033 . . 1043 1047 - BseMII CTCAG 1034 1032 . . 1049 1054 + SfeI* CTRYAG 1049 1053 . . 1053 1056 + SetI ASST 1056 1052 . . 1053 1056 + CviJI RGCY 1054 1054 . . 1053 1056 + AluI AGCT 1054 1054 . . 1055 1059 + DdeI CTNAG 1055 1058 . . 1055 1059 + BspCNI CTCAG 1068 1066 . . 1055 1059 + BseMII CTCAG 1069 1067 . . 1056 1060 + Hpy188I TCNGA 1058 1057 . . 1072 1076 + BlsI GCNGC 1074 1073 . . 1072 1076 + BisI GCNGC 1073 1074 . . 1072 1076 + BbvI GCAGC 1084 1088 . . 1072 1076 + TseI GCWGC 1072 1075 . . 1074 1077 + CviJI RGCY 1075 1075 . . 1080 1083 + Csp6I GTAC 1080 1082 . . 1080 1083 + RsaI GTAC 1081 1081 . . 1082 1086 + Tsp4CI* ACNGT 1084 1083 . . 1085 1088 + Csp6I GTAC 1085 1087 . . 1085 1088 + RsaI GTAC 1086 1086 . . 1087 1091 + Tsp4CI* ACNGT 1089 1088 . . 1084 1094 - BaeI ACNNNNGTAYC 1109 1104 1076 1071 1098 1101 + SetI ASST 1101 1097 . . 1098 1101 + CviJI RGCY 1099 1099 . . 1098 1101 + AluI AGCT 1099 1099 . . 1113 1118 + Hpy178III* TCNNGA 1114 1116 . . 1128 1133 + SmaI CCCGGG 1130 1130 . . 1124 1128 - MboII GAAGA 1116 1115 . . 1128 1133 + Cfr9I CCCGGG 1128 1132 . . 1128 1132 + BssKI CCNGG 1127 1132 . . 1128 1133 + SecI* CCNNGG 1128 1132 . . 1128 1133 + BmeT110I CYCGRG 1129 1131 . . 1128 1132 + ScrFI CCNGG 1129 1130 . . 1128 1132 + CauII* CCSGG 1129 1130 . . 1128 1133 + AvaI CYCGRG 1128 1132 . . 1129 1133 + BssKI CCNGG 1128 1133 . . 1129 1132 + HpaII CCGG 1129 1131 . . 1129 1133 + ScrFI CCNGG 1130 1131 . . 1129 1133 + CauII* CCSGG 1130 1131 . . 1131 1136 + SduI GDGCHC 1135 1131 . . 1131 1136 + BseSI GKGCMC 1135 1131 . . 1132 1137 + NlaIV GGNNCC 1134 1134 . . 1132 1137 + HgiCI* GGYRCC 1132 1136 . . 1150 1154 + TspDTI ATGAA 1165 1163 . . 1152 1156 + MboII GAAGA 1164 1163 . . 1157 1162 + ApoI RAATTY 1157 1161 . . 1158 1161 + TspEI AATT 1157 1161 . . 1164 1168 - BsmAI GTCTC 1158 1162 . . 1176 1179 - MnlI CCTC 1169 1168 . . 1176 1186 - Hin4I GAYNNNNNVTC 1199 1194 1167 1162 1186 1190 + BssKI CCNGG 1185 1190 . . 1186 1190 + BseBI CCWGG 1187 1188 . . 1186 1190 + ScrFI CCNGG 1187 1188 . . 1186 1190 + EcoRII CCWGG 1185 1190 . . 1183 1187 - FokI GGATG 1169 1173 . . 1183 1187 - BseGI GGATG 1182 1180 . . 1188 1191 + SetI ASST 1191 1187 . . 1192 1197 + ApoI RAATTY 1192 1196 . . 1193 1196 + TspEI AATT 1192 1196 . . 1196 1199 + TaqI TCGA 1196 1198 . . 1208 1211 + SetI ASST 1211 1207 . . 1208 1211 + CviJI RGCY 1209 1209 . . 1208 1211 + AluI AGCT 1209 1209 . . 1209 1214 + Cac8I GCNNGC 1211 1211 . . 1209 1214 + BmtI GCTAGC 1213 1209 . . 1209 1214 + NheI GCTAGC 1209 1213 . . 1210 1213 + MaeI CTAG 1210 1212 . . 1212 1215 + SetI ASST 1215 1211 . . 1212 1215 + CviJI RGCY 1213 1213 . . 1212 1215 + AluI AGCT 1213 1213 . . 1220 1223 + CviRI* TGCA 1221 1221 . . 1225 1228 + TaqI TCGA 1225 1227 . . 1221 1226 - BsmI GAATGC 1221 1219 . . 1232 1236 + DdeI CTNAG 1232 1235 . . 1246 1250 + HphI GGTGA 1258 1257 . . 1248 1259 + BdaI TGANNNNNNTCA 1237 1235 1271 1269 1248 1259 - BdaI TGANNNNNNTCA 1271 1269 1237 1235 1267 1270 + CviJI RGCY 1268 1268 . . 1266 1271 - TsoI TARCCA 1256 1254 . . 1272 1275 + FatI CATG 1271 1275 . . 1272 1275 + NlaIII CATG 1275 1271 . . 1272 1275 + CviAII CATG 1272 1274 . . 1281 1287 + TspRI CASTGNN 1287 1278 . . 1282 1286 + BsrI ACTGG 1287 1285 . . 1286 1290 + TfiI GAWTC 1286 1289 . . 1286 1290 + HinfI GANTC 1286 1289 . . 1291 1294 + CviJI RGCY 1292 1292 . . 1294 1297 + FatI CATG 1293 1297 . . 1294 1297 + NlaIII CATG 1297 1293 . . 1294 1297 + CviAII CATG 1294 1296 . . 1304 1314 + Hin4I GAYNNNNNVTC 1295 1290 1327 1322 1302 1306 - BsmAI GTCTC 1296 1300 . . 1313 1318 + Hpy178III* TCNNGA 1314 1316 . . 1304 1314 - Hin4I GAYNNNNNVTC 1327 1322 1295 1290 1321 1324 + MaeI CTAG 1321 1323 . . 1323 1326 + CviJI RGCY 1324 1324 . . 1325 1328 + MnlI CCTC 1335 1334 . . 1327 1330 + TaqI TCGA 1327 1329 . . 1332 1336 - TspDTI ATGAA 1322 1320 . . 1340 1344 - BslFI GGGAC 1325 1329 . . 1348 1352 + Tsp45I GTSAC 1347 1352 . . 1348 1352 + MaeIII GTNAC 1347 1352 . . 1353 1356 + SetI ASST 1356 1352 . . 1358 1363 + HindIII AAGCTT 1358 1362 . . 1359 1362 + SetI ASST 1362 1358 . . 1359 1362 + CviJI RGCY 1360 1360 . . 1359 1362 + AluI AGCT 1360 1360 . . 1374 1384 + Hin4I GAYNNNNNVTC 1365 1360 1397 1392 1374 1378 - BccI CCATC 1368 1369 . . 1382 1387 + Hpy166II GTNNAC 1384 1384 . . 1374 1384 - Hin4I GAYNNNNNVTC 1397 1392 1365 1360 1387 1397 + BsiYI* CCNNNNNNNGG 1393 1390 . . 1388 1394 + TspRI CASTGNN 1394 1385 . . 1387 1391 - BsrI ACTGG 1387 1385 . . 1393 1397 - Hin4II* CCTTC 1387 1386 . . 1402 1406 + TfiI GAWTC 1402 1405 . . 1399 1402 - MnlI CCTC 1392 1391 . . 1402 1406 + HinfI GANTC 1402 1405 . . 1409 1412 + TaqI TCGA 1409 1411 . . 1411 1415 + PleI GAGTC 1419 1420 . . 1411 1415 + MlyI GAGTC 1420 1420 . . 1411 1415 + HinfI GANTC 1411 1414 . . 1414 1418 + Hpy188I TCNGA 1416 1415 . . 1416 1420 + BmgT120I GGNCC 1417 1418 . . 1416 1420 + AvaII GGWCC 1416 1419 . . 1416 1420 + AsuI* GGNCC 1416 1419 . . 1413 1418 - MmeI TCCRAC 1394 1392 . . 1428 1431 + TaiI ACGT 1431 1427 . . 1428 1431 + SetI ASST 1431 1427 . . 1428 1431 + MaeII ACGT 1428 1430 . . 1430 1434 + MaeIII GTNAC 1429 1434 . . 1436 1440 + BccI CCATC 1444 1445 . . 1437 1441 - FokI GGATG 1423 1427 . . 1441 1444 + FatI CATG 1440 1444 . . 1441 1444 + NlaIII CATG 1444 1440 . . 1441 1444 + CviAII CATG 1441 1443 . . 1437 1441 - BseGI GGATG 1436 1434 . . 1442 1446 + TspDTI ATGAA 1457 1455 . . 1450 1453 + MseI TTAA 1450 1452 . . 1452 1455 + TspEI AATT 1451 1455 . . 1462 1467 + BsrDI GCAATG 1469 1467 . . 1469 1475 + TspRI CASTGNN 1475 1466 . . 1470 1474 + Tsp4CI* ACNGT 1472 1471 . . 1477 1481 - BsmAI GTCTC 1471 1475 . . 1484 1487 + MseI TTAA 1484 1486 . . 1495 1499 - Hin4II* CCTTC 1489 1488 . . 1514 1517 + CviJI RGCY 1515 1515 . . 1516 1519 + MnlI CCTC 1526 1525 . . 1517 1522 + SmlI CTYRAG 1517 1521 . . 1521 1524 + SetI ASST 1524 1520 . . 1517 1522 - Bce83I* CTTGAG 1502 1500 . . 1522 1527 + NlaIV GGNNCC 1524 1524 . . 1522 1527 + HgiCI* GGYRCC 1522 1526 . . 1527 1530 + FatI CATG 1526 1530 . . 1527 1530 + NlaIII CATG 1530 1526 . . 1527 1530 + CviAII CATG 1527 1529 . . 1541 1545 + DdeI CTNAG 1541 1544 . . 1543 1553 + FalI AAGNNNNNCTT 1534 1529 1566 1561 1543 1553 - FalI AAGNNNNNCTT 1566 1561 1534 1529 1554 1557 + SetI ASST 1557 1553 . . 1569 1574 + Hpy178III* TCNNGA 1570 1572 . . 1575 1578 + SetI ASST 1578 1574 . . 1575 1578 + CviJI RGCY 1576 1576 . . 1575 1578 + AluI AGCT 1576 1576 . . 1576 1581 + Cac8I GCNNGC 1578 1578 . . 1584 1588 + DdeI CTNAG 1584 1587 . . 1590 1595 + Hpy166II GTNNAC 1592 1592 . . 1590 1595 + HpaI GTTAAC 1592 1592 . . 1590 1595 + HindII GTYRAC 1592 1592 . . 1591 1594 + MseI TTAA 1591 1593 . . 1596 1601 + Hpy178III* TCNNGA 1597 1599 . . 1596 1601 + BetI* WCCGGW 1596 1600 . . 1596 1601 + BspMII* TCCGGA 1596 1600 . . 1597 1600 + HpaII CCGG 1597 1599 . . 1599 1603 + FokI GGATG 1612 1616 . . 1599 1603 + BseGI GGATG 1605 1603 . . 1601 1605 + TspDTI ATGAA 1616 1614 . . 1603 1608 + ApoI RAATTY 1603 1607 . . 1604 1607 + TspEI AATT 1603 1607 . . 1627 1631 + TfiI GAWTC 1627 1630 . . 1627 1631 + HinfI GANTC 1627 1630 . . 1633 1637 + BsrI ACTGG 1638 1636 . . 1642 1645 + CviRI* TGCA 1643 1643 . . 1644 1650 + TspRI CASTGNN 1650 1641 . . 1645 1649 + Tsp4CI* ACNGT 1647 1646 . . 1651 1655 + BssKI CCNGG 1650 1655 . . 1651 1655 + ScrFI CCNGG 1652 1653 . . 1651 1655 + CauII* CCSGG 1652 1653 . . 1651 1661 + BsiYI* CCNNNNNNNGG 1657 1654 . . 1652 1655 + HpaII CCGG 1652 1654 . . 1652 1662 + BsiYI* CCNNNNNNNGG 1658 1655 . . 1668 1673 + Hpy178III* TCNNGA 1669 1671 . . 1674 1677 + SetI ASST 1677 1673 . . 1674 1680 + SexAI ACCWGGT 1674 1679 . . 1675 1679 + BssKI CCNGG 1674 1679 . . 1675 1679 + BseBI CCWGG 1676 1677 . . 1675 1679 + ScrFI CCNGG 1676 1677 . . 1675 1679 + EcoRII CCWGG 1674 1679 . . 1679 1682 + Csp6I GTAC 1679 1681 . . 1679 1682 + RsaI GTAC 1680 1680 . . 1690 1693 + MseI TTAA 1690 1692 . . 1707 1712 + NlaIV GGNNCC 1709 1709 . . 1707 1712 + HgiCI* GGYRCC 1707 1711 . . 1710 1713 + SetI ASST 1713 1709 . . 1710 1716 + SexAI ACCWGGT 1710 1715 . . 1711 1715 + BssKI CCNGG 1710 1715 . . 1711 1715 + BseBI CCWGG 1712 1713 . . 1711 1715 + ScrFI CCNGG 1712 1713 . . 1711 1715 + EcoRII CCWGG 1710 1715 . . 1716 1721 + Hpy178III* TCNNGA 1717 1719 . . 1727 1730 + MseI TTAA 1727 1729 . . 1753 1758 + ApoI RAATTY 1753 1757 . . 1754 1757 + TspEI AATT 1753 1757 . . 1769 1773 + Tsp4CI* ACNGT 1771 1770 . . 1772 1776 + Tsp45I GTSAC 1771 1776 . . 1772 1776 + MaeIII GTNAC 1771 1776 . . #--------------------------------------- # # # AciI: # [CCGC] # 87 320 456 916 # # AflIII: # [ACRYGT] # 287 1492 # # AhaIII*: # [TTTAAA] # 597 1182 # # AjuI: # [GAANNNNNNNTTGG] # 10 32 1737 # # AluI: # [AGCT] # 4 15 18 24 37 45 # 93 110 138 147 157 158 # 203 205 209 216 # # AlwNI: # [CAGNNNCTG] # 749 1030 # # ApoI: # [RAATTY] # 26 35 150 209 242 411 # 706 # # AsuI*: # [GGNCC] # 363 602 814 # # AsuII: # [TTCGAA] # 285 301 1193 # # AvaI: # [CYCGRG] # 651 1128 # # AvaII: # [GGWCC] # 363 1416 # # BaeI: # [ACNNNNGTAYC] # 33 670 1076 # # BamHI: # [GGATCC] # 432 1347 # # BarI: # [GAAGNNNNNNTAC] # 32 355 1392 # # BbvCI: # [CCTCAGC] # 132 353 1294 # # BbvI: # [GCAGC] # 205 294 585 695 # # BbvII*: # [GAAGAC] # 347 1432 # # BccI: # [CCATC] # 76 335 397 971 # # Bce83I*: # [CTTGAG] # 216 277 630 656 # # BceAI: # [ACGGC] # 361 1418 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 508 1237 # # BetI*: # [WCCGGW] # 183 1596 # # BinI*: # [GGATC] # 13 427 1339 # # BisI: # [GCNGC] # 205 294 574 706 # # BlsI: # [GCNGC] # 205 294 575 705 # # BmeT110I: # [CYCGRG] # 650 1129 # # BmgT120I: # [GGNCC] # 362 602 815 # # BmtI: # [GCTAGC] # 566 1213 # # Bpu10I: # [CCTNAGC] # 132 353 1294 # # BseBI: # [CCWGG] # 36 67 174 201 489 812 # # BseGI: # [GGATG] # 169 174 254 383 389 410 # # BseMII: # [CTCAG] # 35 146 353 535 710 # # BseSI: # [GKGCMC] # 644 1135 # # BsiYI*: # [CCNNNNNNNGG] # 1 121 264 570 823 # # BslFI: # [GGGAC] # 454 589 736 # # BsmAI: # [GTCTC] # 138 175 180 308 356 622 # # BsmI: # [GAATGC] # 558 1221 # # BspCNI: # [CTCAG] # 33 145 353 537 711 # # BspMI: # [ACCTGC] # 266 1513 # # BspMII*: # [TCCGGA] # 183 1596 # # BsrDI: # [GCAATG] # 310 1469 # # BsrI: # [ACTGG] # 100 137 141 251 291 859 # # BssKI: # [CCNGG] # 1 24 36 57 69 116 # 199 465 812 # # BstKTI: # [GATC] # 435 1344 # # Cac8I: # [GCNNGC] # 201 367 1211 # # CauII*: # [CCSGG] # 1 127 522 1129 # # Cfr9I: # [CCCGGG] # 651 1128 # # Csp6I: # [GTAC] # 5 100 143 229 594 708 # # CviAII: # [CATG] # 22 86 147 252 1272 # # CviJI: # [RGCY] # 4 15 18 21 21 24 # 24 24 32 35 36 36 # 37 55 58 61 63 75 # 110 136 155 158 173 203 # 205 # # CviRI*: # [TGCA] # 136 153 422 449 619 # # DdeI: # [CTNAG] # 12 43 117 132 177 195 # 236 309 558 # # DpnI: # [GATC] # 434 1345 # # Eco57I: # [CTGAAG] # 425 1354 # # Eco57MI: # [CTGRAG] # 20 425 1334 # # EcoP15I: # [CAGCAG] # 135 601 1043 # # EcoRI: # [GAATTC] # 864 915 # # EcoRII: # [CCWGG] # 36 69 174 199 489 812 # # EcoRV: # [GATATC] # 98 639 1042 # # FalI: # [AAGNNNNNCTT] # 0 0 32 213 1534 # # FatI: # [CATG] # 22 86 147 253 1271 # # FauI: # [CCCGC] # 327 1452 # # FokI: # [GGATG] # 167 189 254 376 383 410 # # GlaI: # [GCGC] # 93 1686 # # GsuI: # [CTGGAG] # 445 1334 # # HaeIII: # [GGCC] # 816 963 # # HgaI: # [GACGC] # 250 1529 # # HgiCI*: # [GGYRCC] # 72 185 385 390 747 # # HhaI: # [GCGC] # 94 1685 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 18 32 32 32 32 32 # 32 38 96 147 195 382 # 711 # # Hin4II*: # [CCTTC] # 45 66 102 253 290 357 # 666 # # Hin6I: # [GCGC] # 92 1687 # # HindII: # [GTYRAC] # 187 663 929 # # HindIII: # [AAGCTT] # 155 421 468 735 # # HinfI: # [GANTC] # 9 78 116 152 192 211 # 216 241 251 313 # # HpaI: # [GTTAAC] # 187 1592 # # HpaII: # [CCGG] # 55 127 468 1129 # # HphI: # [GGTGA] # 227 521 1031 # # Hpy166II: # [GTNNAC] # 187 208 455 929 # # Hpy178III*: # [TCNNGA] # 27 48 62 72 106 162 # 177 198 200 256 471 # # Hpy188I: # [TCNGA] # 358 363 509 549 # # Ksp632I*: # [CTCTTC] # 43 201 468 1067 # # MaeI: # [CTAG] # 24 111 126 213 270 458 # 577 # # MaeII: # [ACGT] # 53 55 64 172 271 351 # 813 # # MaeIII: # [GTNAC] # 8 82 342 1347 # # MboI: # [GATC] # 432 1347 # # MboII: # [GAAGA] # 3 10 48 48 66 70 # 116 164 188 223 228 615 # # MfeI: # [CAATTG] # 328 1451 # # MlyI: # [GAGTC] # 72 359 366 982 # # MmeI: # [TCCRAC] # 385 1394 # # MnlI: # [CCTC] # 21 28 48 49 57 64 # 70 103 134 141 166 198 # 212 235 253 # # MseI: # [TTAA] # 15 17 34 37 40 52 # 59 92 99 107 405 822 # # MslI: # [CAYNNNNRTG] # 394 1385 # # MwoI: # [GCNNNNNNNGC] # 93 373 405 908 # # NheI: # [GCTAGC] # 570 1209 # # NlaIII: # [CATG] # 22 86 147 249 1275 # # NlaIV: # [GGNNCC] # 47 70 185 387 390 700 # # NspBII*: # [CMGCKG] # 781 998 # # PfoI: # [TCCNGGA] # 199 1580 # # PleI: # [GAGTC] # 72 360 366 981 # # PmeI: # [GTTTAAAC] # 597 1182 # # PshAI: # [GACNNNNGTC] # 260 1519 # # PsiI: # [TTATAA] # 712 1067 # # PvuII: # [CAGCTG] # 781 998 # # RsaI: # [GTAC] # 5 99 144 229 594 708 # # SapI: # [GCTCTTC] # 201 1578 # # ScrFI: # [CCNGG] # 1 24 36 57 67 116 # 201 465 812 # # SduI: # [GDGCHC] # 644 1135 # # SecI*: # [CCNNGG] # 651 1128 # # SetI: # [ASST] # 4 6 9 12 12 15 # 15 18 18 20 20 21 # 26 27 30 32 32 32 # 33 36 37 40 40 45 # 51 55 56 60 66 66 # 69 77 87 90 93 93 # 96 99 141 # # SexAI: # [ACCWGGT] # 36 69 1674 # # SfaNI: # [GCATC] # 187 594 998 # # SfeI*: # [CTRYAG] # 27 33 181 182 291 335 # 730 # # SmaI: # [CCCGGG] # 649 1130 # # SmlI: # [CTYRAG] # 216 262 630 671 # # SspI: # [AATATT] # 474 1305 # # TaiI: # [ACGT] # 55 56 64 172 271 348 # 813 # # TaqI: # [TCGA] # 29 82 102 285 301 370 # 610 # # TatI: # [WGTACW] # 142 1637 # # TfiI: # [GAWTC] # 116 152 225 251 270 313 # 452 # # TseI: # [GCWGC] # 205 294 573 707 # # TsoI: # [TARCCA] # 265 523 991 # # Tsp45I: # [GTSAC] # 8 424 1347 # # Tsp4CI*: # [ACNGT] # 5 8 11 22 39 91 # 124 175 383 921 # # TspDTI: # [ATGAA] # 135 147 157 159 163 218 # 800 # # TspEI: # [AATT] # 14 26 29 33 35 39 # 39 43 80 81 117 150 # 152 180 213 259 289 # # TspRI: # [CASTGNN] # 14 24 81 107 129 175 # 1249 # # XhoII: # [RGATCY] # 432 1347 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1779 # Reported_sequences: 1 # Reported_hitcount: 469 #---------------------------------------