Restriction Fragments for YLLCdelta1



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YLLCdelta1 on chromosome XII from coordinates 92381 to 92050.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.29695.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 40 45 + PsiI TTATAA 42 42 . . 66 70 + Tsp4CI* ACNGT 68 67 . . 86 89 + SetI ASST 89 85 . . 97 100 + SetI ASST 100 96 . . 97 100 + CviJI RGCY 98 98 . . 97 100 + AluI AGCT 98 98 . . 93 103 - Hin4I GAYNNNNNVTC 116 111 84 79 105 108 + TaqI TCGA 105 107 . . 108 111 + SetI ASST 111 107 . . 107 110 - MnlI CCTC 100 99 . . 114 117 - MnlI CCTC 107 106 . . 119 122 + SetI ASST 122 118 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + AluI AGCT 120 120 . . 121 126 + SfeI* CTRYAG 121 125 . . 154 158 + TspDTI ATGAA 169 167 . . 168 172 + TspGWI ACGGA 183 181 . . 175 178 - MnlI CCTC 168 167 . . 175 185 - Hin4I GAYNNNNNVTC 198 193 166 161 188 193 + SspI AATATT 190 190 . . 204 209 + SspI AATATT 206 206 . . 210 214 + TfiI GAWTC 210 213 . . 210 214 + HinfI GANTC 210 213 . . 214 224 + BsiYI* CCNNNNNNNGG 220 217 . . 224 228 + TfiI GAWTC 224 227 . . 221 224 - MnlI CCTC 214 213 . . 224 228 + HinfI GANTC 224 227 . . 231 236 + XhoII RGATCY 231 235 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 235 242 + PspXI VCTCGAGB 236 240 . . 235 238 + MnlI CCTC 245 244 . . 235 242 + AbsI CCTCGAGG 236 240 . . 236 241 + SmlI CTYRAG 236 240 . . 236 241 + XhoI CTCGAG 236 240 . . 236 241 + BmeT110I CYCGRG 237 239 . . 236 241 + AvaI CYCGRG 236 240 . . 232 236 - BinI* GGATC 226 227 . . 237 240 + TaqI TCGA 237 239 . . 239 244 + BseRI GAGGAG 254 252 . . 239 242 - MnlI CCTC 232 231 . . 250 253 + MaeI CTAG 250 252 . . 262 267 + Hpy166II GTNNAC 264 264 . . 262 267 + BssNAI GTATAC 264 264 . . 262 267 + AccI GTMKAC 263 265 . . 266 269 + SetI ASST 269 265 . . 270 275 + SspI AATATT 272 272 . . 278 281 + CviJI RGCY 279 279 . . 286 289 + MseI TTAA 286 288 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . 309 312 + SetI ASST 312 308 . . 310 313 + MnlI CCTC 320 319 . . 315 320 + ApoI RAATTY 315 319 . . 316 319 + TspEI AATT 315 319 . . 319 323 - HphI GGTGA 311 310 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 236 # # AccI: # [GTMKAC] # 69 263 # # AluI: # [AGCT] # 22 98 212 # # ApoI: # [RAATTY] # 17 315 # # AvaI: # [CYCGRG] # 96 236 # # BinI*: # [GGATC] # 106 226 # # BmeT110I: # [CYCGRG] # 95 237 # # BseRI: # [GAGGAG] # 78 254 # # BsiYI*: # [CCNNNNNNNGG] # 112 220 # # BssNAI: # [GTATAC] # 68 264 # # BstKTI: # [GATC] # 98 234 # # CviJI: # [RGCY] # 22 53 98 159 # # DpnI: # [GATC] # 99 233 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 84 134 # # HinfI: # [GANTC] # 14 37 71 210 # # HphI: # [GGTGA] # 21 311 # # Hpy166II: # [GTNNAC] # 68 264 # # MaeI: # [CTAG] # 82 250 # # MboI: # [GATC] # 101 231 # # MnlI: # [CCTC] # 7 12 13 18 46 61 # 75 100 # # MseI: # [TTAA] # 46 286 # # PsiI: # [TTATAA] # 42 290 # # PspXI: # [VCTCGAGB] # 96 236 # # SetI: # [ASST] # 11 11 11 20 43 89 # 147 # # SfeI*: # [CTRYAG] # 121 211 # # SmlI: # [CTYRAG] # 96 236 # # SspI: # [AATATT] # 16 60 66 190 # # TaqI: # [TCGA] # 95 105 132 # # TfiI: # [GAWTC] # 14 37 71 210 # # Tsp4CI*: # [ACNGT] # 68 264 # # TspDTI: # [ATGAA] # 163 169 # # TspEI: # [AATT] # 11 17 304 # # TspGWI: # [ACGGA] # 149 183 # # XhoI: # [CTCGAG] # 96 236 # # XhoII: # [RGATCY] # 101 231 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 332 # Reported_sequences: 1 # Reported_hitcount: 58 #---------------------------------------