Restriction Fragments for ATG10/YLL042C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ATG10/YLL042C on chromosome XII from coordinates 52590 to 52087.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30583.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + TfiI GAWTC 3 6 . . 3 7 + HinfI GANTC 3 6 . . 12 16 + BssKI CCNGG 11 16 . . 12 16 + BseBI CCWGG 13 14 . . 12 16 + ScrFI CCNGG 13 14 . . 12 16 + EcoRII CCWGG 11 16 . . 24 29 + TsoI TARCCA 40 38 . . 25 28 + CviJI RGCY 26 26 . . 32 35 + CviRI* TGCA 33 33 . . 46 50 + HinfI GANTC 46 49 . . 46 50 - PleI GAGTC 40 41 . . 46 50 - MlyI GAGTC 40 40 . . 60 70 + BsiYI* CCNNNNNNNGG 66 63 . . 65 69 + BsrI ACTGG 70 68 . . 65 70 + BfiI ACTGGG 75 74 . . 68 74 + DraII RGGNCCY 69 72 . . 68 73 + SduI GDGCHC 72 68 . . 68 73 + Bsp120I GGGCCC 68 72 . . 68 73 + NlaIV GGNNCC 70 70 . . 68 72 + BmgT120I GGNCC 69 70 . . 68 73 + HgiJII* GRGCYC 72 68 . . 68 73 + BseSI GKGCMC 72 68 . . 68 72 + AsuI* GGNCC 68 71 . . 68 73 + ApaI GGGCCC 72 68 . . 69 72 + CviJI RGCY 70 70 . . 69 72 + HaeIII GGCC 70 70 . . 69 73 + BmgT120I GGNCC 70 71 . . 69 73 + AsuI* GGNCC 69 72 . . 88 97 + MslI CAYNNNNRTG 92 92 . . 98 102 + TspDTI ATGAA 113 111 . . 105 109 + FokI GGATG 118 122 . . 105 109 + BseGI GGATG 111 109 . . 106 110 - BccI CCATC 100 101 . . 124 127 + MseI TTAA 124 126 . . 129 132 + SetI ASST 132 128 . . 135 138 + TaqI TCGA 135 137 . . 142 147 + PstI CTGCAG 146 142 . . 142 147 + SfeI* CTRYAG 142 146 . . 143 146 + CviRI* TGCA 144 144 . . 155 159 + DdeI CTNAG 155 158 . . 155 159 - BspCNI CTCAG 147 145 . . 155 159 - BseMII CTCAG 146 144 . . 173 176 + TspEI AATT 172 176 . . 191 194 + TspEI AATT 190 194 . . 223 228 + SduI GDGCHC 227 223 . . 223 228 + HgiJII* GRGCYC 227 223 . . 224 227 + CviJI RGCY 225 225 . . 250 255 + BseRI GAGGAG 265 263 . . 250 253 - MnlI CCTC 243 242 . . 265 269 - BccI CCATC 259 260 . . 284 287 + TspEI AATT 283 287 . . 288 292 - SfaNI GCATC 278 282 . . 301 311 + Hin4I GAYNNNNNVTC 292 287 324 319 301 306 + EcoRV GATATC 303 303 . . 305 308 + TaqI TCGA 305 307 . . 307 311 + TfiI GAWTC 307 310 . . 307 311 + HinfI GANTC 307 310 . . 301 311 - Hin4I GAYNNNNNVTC 324 319 292 287 312 316 + BlsI GCNGC 314 313 . . 312 316 + BisI GCNGC 313 314 . . 312 316 + TseI GCWGC 312 315 . . 312 316 - BbvI GCAGC 299 303 . . 331 336 + ApoI RAATTY 331 335 . . 332 335 + TspEI AATT 331 335 . . 335 340 + MmeI TCCRAC 360 358 . . 340 343 + MaeI CTAG 340 342 . . 342 345 + SetI ASST 345 341 . . 348 353 - BciVI GTATCC 342 341 . . 359 362 + MseI TTAA 359 361 . . 362 365 + SetI ASST 365 361 . . 363 367 + BssKI CCNGG 362 367 . . 363 367 + BseBI CCWGG 364 365 . . 363 367 + ScrFI CCNGG 364 365 . . 363 367 + EcoRII CCWGG 362 367 . . 369 372 + SetI ASST 372 368 . . 367 371 - Hin4II* CCTTC 361 360 . . 390 394 + BccI CCATC 398 399 . . 391 395 - FokI GGATG 377 381 . . 395 398 + FatI CATG 394 398 . . 395 398 + NlaIII CATG 398 394 . . 395 398 + CviAII CATG 395 397 . . 391 395 - BseGI GGATG 390 388 . . 407 410 + FatI CATG 406 410 . . 407 410 + NlaIII CATG 410 406 . . 407 410 + CviAII CATG 407 409 . . 417 420 + SetI ASST 420 416 . . 418 424 + BstEII GGTNACC 418 423 . . 418 422 + HphI GGTGA 430 429 . . 419 423 + Tsp45I GTSAC 418 423 . . 419 423 + MaeIII GTNAC 418 423 . . 423 427 + BssKI CCNGG 422 427 . . 423 427 + BseBI CCWGG 424 425 . . 423 427 + ScrFI CCNGG 424 425 . . 423 427 + EcoRII CCWGG 422 427 . . 426 429 + CviJI RGCY 427 427 . . 428 432 + DdeI CTNAG 428 431 . . 428 432 - BspCNI CTCAG 420 418 . . 428 432 - BseMII CTCAG 419 417 . . 435 438 + FatI CATG 434 438 . . 435 438 + NlaIII CATG 438 434 . . 435 438 + CviAII CATG 435 437 . . 433 437 - TspDTI ATGAA 423 421 . . 438 443 + Hpy166II GTNNAC 440 440 . . 438 443 + HindII GTYRAC 440 440 . . 430 440 - Hin4I GAYNNNNNVTC 453 448 421 416 447 452 + SmlI CTYRAG 447 451 . . 447 452 + AflII CTTAAG 447 451 . . 448 451 + MseI TTAA 448 450 . . 452 462 + Hin4I GAYNNNNNVTC 443 438 475 470 452 462 + Eam1105I GACNNNNNGTC 457 456 . . 455 459 - BccI CCATC 449 450 . . 452 462 - Hin4I GAYNNNNNVTC 475 470 443 438 469 473 - TspDTI ATGAA 459 457 . . 481 486 - TsoI TARCCA 471 469 . . 486 489 + SetI ASST 489 485 . . 496 500 + TfiI GAWTC 496 499 . . 496 500 + HinfI GANTC 496 499 . . 498 502 - TspDTI ATGAA 488 486 . . #--------------------------------------- # # # AflII: # [CTTAAG] # 57 447 # # ApaI: # [GGGCCC] # 72 432 # # ApoI: # [RAATTY] # 173 331 # # AsuI*: # [GGNCC] # 1 68 435 # # BbvI: # [GCAGC] # 205 299 # # BccI: # [CCATC] # 51 55 100 139 159 # # BciVI: # [GTATCC] # 162 342 # # BfiI: # [ACTGGG] # 75 429 # # BisI: # [GCNGC] # 191 313 # # BlsI: # [GCNGC] # 190 314 # # BmgT120I: # [GGNCC] # 1 69 434 # # BseBI: # [CCWGG] # 13 60 80 351 # # BseGI: # [GGATG] # 111 114 279 # # BseMII: # [CTCAG] # 85 146 273 # # BseRI: # [GAGGAG] # 239 265 # # BseSI: # [GKGCMC] # 72 432 # # BsiYI*: # [CCNNNNNNNGG] # 66 438 # # Bsp120I: # [GGGCCC] # 68 436 # # BspCNI: # [CTCAG] # 84 147 273 # # BsrI: # [ACTGG] # 70 434 # # BssKI: # [CCNGG] # 11 60 82 351 # # BstEII: # [GGTNACC] # 86 418 # # CviAII: # [CATG] # 12 28 69 395 # # CviJI: # [RGCY] # 26 44 77 155 202 # # CviRI*: # [TGCA] # 33 111 360 # # DdeI: # [CTNAG] # 76 155 273 # # DraII: # [RGGNCCY] # 69 435 # # Eam1105I: # [GACNNNNNGTC] # 47 457 # # EcoRII: # [CCWGG] # 11 60 82 351 # # EcoRV: # [GATATC] # 201 303 # # FatI: # [CATG] # 12 28 70 394 # # FokI: # [GGATG] # 118 127 259 # # HaeIII: # [GGCC] # 70 434 # # HgiJII*: # [GRGCYC] # 72 155 277 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 10 22 # 22 29 32 97 292 # # Hin4II*: # [CCTTC] # 143 361 # # HindII: # [GTYRAC] # 64 440 # # HinfI: # [GANTC] # 3 8 43 189 261 # # HphI: # [GGTGA] # 74 430 # # Hpy166II: # [GTNNAC] # 64 440 # # MaeI: # [CTAG] # 164 340 # # MaeIII: # [GTNAC] # 86 418 # # MlyI: # [GAGTC] # 40 464 # # MmeI: # [TCCRAC] # 144 360 # # MnlI: # [CCTC] # 243 261 # # MseI: # [TTAA] # 56 89 124 235 # # MslI: # [CAYNNNNRTG] # 92 412 # # NlaIII: # [CATG] # 12 28 66 398 # # NlaIV: # [GGNNCC] # 70 434 # # PleI: # [GAGTC] # 40 464 # # PstI: # [CTGCAG] # 146 358 # # ScrFI: # [CCNGG] # 13 60 80 351 # # SduI: # [GDGCHC] # 72 155 277 # # SetI: # [ASST] # 7 15 20 48 69 132 # 213 # # SfaNI: # [GCATC] # 226 278 # # SfeI*: # [CTRYAG] # 142 362 # # SmlI: # [CTYRAG] # 57 447 # # TaqI: # [TCGA] # 135 170 199 # # TfiI: # [GAWTC] # 3 8 189 304 # # TseI: # [GCWGC] # 192 312 # # TsoI: # [TARCCA] # 33 40 431 # # Tsp45I: # [GTSAC] # 86 418 # # TspDTI: # [ATGAA] # 16 29 36 113 310 # # TspEI: # [AATT] # 18 48 93 172 173 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 504 # Reported_sequences: 1 # Reported_hitcount: 118 #---------------------------------------