Restriction Fragments for UBI4/YLL039C



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The currently selected gene/sequence is :

UBI4/YLL039C on chromosome XII from coordinates 65207 to 64062.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24778.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 14 19 + Hpy178III* TCNNGA 15 17 . . 25 30 + Cfr10I RCCGGY 25 29 . . 25 30 + BetI* WCCGGW 25 29 . . 25 30 + AgeI ACCGGT 25 29 . . 26 29 + HpaII CCGG 26 28 . . 35 46 + BstXI CCANNNNNNTGG 42 38 . . 52 56 + TfiI GAWTC 52 55 . . 52 56 + HinfI GANTC 52 55 . . 58 62 + Hpy188I TCNGA 60 59 . . 55 59 - MboII GAAGA 47 46 . . 65 69 + BccI CCATC 73 74 . . 68 71 + TaqI TCGA 68 70 . . 73 78 + AclI AACGTT 74 76 . . 74 77 + TaiI ACGT 77 73 . . 74 77 + SetI ASST 77 73 . . 74 77 + MaeII ACGT 74 76 . . 77 80 + MseI TTAA 77 79 . . 82 85 + TaqI TCGA 82 84 . . 86 91 + ApoI RAATTY 86 90 . . 87 90 + TspEI AATT 86 90 . . 90 95 + Hpy178III* TCNNGA 91 93 . . 102 105 + SetI ASST 105 101 . . 100 104 - Hin4II* CCTTC 94 93 . . 104 109 + BciVI GTATCC 115 114 . . 109 112 + MnlI CCTC 119 118 . . 111 116 + Hpy178III* TCNNGA 112 114 . . 110 115 - Eco57MI CTGRAG 95 93 . . 115 118 + DpnI GATC 116 116 . . 115 118 + BstKTI GATC 117 115 . . 110 115 - GsuI CTGGAG 95 93 . . 115 118 + MboI GATC 114 118 . . 129 132 + DpnI GATC 130 130 . . 129 132 + BstKTI GATC 131 129 . . 129 132 + MboI GATC 128 132 . . 136 141 + Cfr10I RCCGGY 136 140 . . 137 140 + HpaII CCGG 137 139 . . 144 148 + BlsI GCNGC 146 145 . . 144 148 + BisI GCNGC 145 146 . . 144 148 + BbvI GCAGC 156 160 . . 144 148 + TseI GCWGC 144 147 . . 146 149 + SetI ASST 149 145 . . 146 149 + CviJI RGCY 147 147 . . 146 149 + AluI AGCT 147 147 . . 148 151 + MaeI CTAG 148 150 . . 151 155 + MboII GAAGA 163 162 . . 151 156 + BbvII* GAAGAC 158 162 . . 155 159 + Tsp4CI* ACNGT 157 156 . . 169 173 + Hpy188I TCNGA 171 170 . . 183 187 + Hpy188I TCNGA 185 184 . . 190 194 + PleI GAGTC 198 199 . . 190 194 + MlyI GAGTC 199 199 . . 186 190 - Hin4II* CCTTC 180 179 . . 190 194 + HinfI GANTC 190 193 . . 192 197 + Hpy166II GTNNAC 194 194 . . 196 199 + SetI ASST 199 195 . . 211 215 + DdeI CTNAG 211 214 . . 215 218 + CviJI RGCY 216 216 . . 210 216 - Bpu10I CCTNAGC 211 214 . . 217 221 + DdeI CTNAG 217 220 . . 222 225 + SetI ASST 225 221 . . 221 224 - MnlI CCTC 214 213 . . 230 233 + CviRI* TGCA 231 231 . . 233 238 + XhoII RGATCY 233 237 . . 233 238 + BglII AGATCT 233 237 . . 234 237 + DpnI GATC 235 235 . . 234 237 + BstKTI GATC 236 234 . . 234 237 + MboI GATC 233 237 . . 242 245 + MseI TTAA 242 244 . . 253 258 + Cfr10I RCCGGY 253 257 . . 253 258 + BetI* WCCGGW 253 257 . . 253 258 + AgeI ACCGGT 253 257 . . 254 257 + HpaII CCGG 254 256 . . 263 267 + BccI CCATC 271 272 . . 275 278 + SetI ASST 278 274 . . 274 277 - MnlI CCTC 267 266 . . 280 284 + TfiI GAWTC 280 283 . . 280 284 + HinfI GANTC 280 283 . . 284 287 + MnlI CCTC 294 293 . . 274 284 - Hin4I GAYNNNNNVTC 297 292 265 260 286 291 + MmeI TCCRAC 311 309 . . 286 290 + Hpy188I TCNGA 288 287 . . 293 298 + McrI* CGRYCG 296 294 . . 293 298 + PvuI CGATCG 296 294 . . 294 297 + DpnI GATC 295 295 . . 294 297 + BstKTI GATC 296 294 . . 294 297 + MboI GATC 293 297 . . 295 300 + ClaI ATCGAT 296 298 . . 296 299 + TaqI TCGA 296 298 . . 301 306 + AclI AACGTT 302 304 . . 302 305 + TaiI ACGT 305 301 . . 302 305 + SetI ASST 305 301 . . 302 305 + MaeII ACGT 302 304 . . 305 308 + MseI TTAA 305 307 . . 310 313 + TaqI TCGA 310 312 . . 317 323 + PfoI TCCNGGA 317 322 . . 318 322 + BssKI CCNGG 317 322 . . 318 322 + BseBI CCWGG 319 320 . . 318 322 + ScrFI CCNGG 319 320 . . 318 322 + EcoRII CCWGG 317 322 . . 318 328 + BsiYI* CCNNNNNNNGG 324 321 . . 330 333 + SetI ASST 333 329 . . 328 332 - Hin4II* CCTTC 322 321 . . 332 337 + BciVI GTATCC 343 342 . . 337 340 + MnlI CCTC 347 346 . . 339 344 + Hpy178III* TCNNGA 340 342 . . 339 344 + BetI* WCCGGW 339 343 . . 339 344 + BspMII* TCCGGA 339 343 . . 340 343 + HpaII CCGG 340 342 . . 342 346 + BinI* GGATC 350 351 . . 343 346 + DpnI GATC 344 344 . . 343 346 + BstKTI GATC 345 343 . . 343 346 + MboI GATC 342 346 . . 352 355 + SetI ASST 355 351 . . 351 354 - MnlI CCTC 344 343 . . 357 360 + DpnI GATC 358 358 . . 357 360 + BstKTI GATC 359 357 . . 357 360 + MboI GATC 356 360 . . 364 369 + Cfr10I RCCGGY 364 368 . . 365 368 + HpaII CCGG 365 367 . . 372 376 + BlsI GCNGC 374 373 . . 372 376 + BisI GCNGC 373 374 . . 372 376 + BbvI GCAGC 384 388 . . 372 376 + TseI GCWGC 372 375 . . 374 377 + SetI ASST 377 373 . . 374 377 + CviJI RGCY 375 375 . . 374 377 + AluI AGCT 375 375 . . 376 379 + MaeI CTAG 376 378 . . 379 383 + MboII GAAGA 391 390 . . 382 386 - BccI CCATC 376 377 . . 391 394 + SetI ASST 394 390 . . 397 401 + Hpy188I TCNGA 399 398 . . 408 412 - FokI GGATG 394 398 . . 408 412 - BseGI GGATG 407 405 . . 418 422 + TfiI GAWTC 418 421 . . 418 422 + HinfI GANTC 418 421 . . 425 430 + Ksp632I* CTCTTC 431 434 . . 426 430 - MboII GAAGA 418 417 . . 430 438 + DraIII CACNNNGTG 435 432 . . 445 449 + DdeI CTNAG 445 448 . . 441 445 - BsmAI GTCTC 435 439 . . 445 449 - BspCNI CTCAG 437 435 . . 445 449 - BseMII CTCAG 436 434 . . 450 453 + SetI ASST 453 449 . . 449 452 - MnlI CCTC 442 441 . . 458 461 + CviRI* TGCA 459 459 . . 461 466 + ApoI RAATTY 461 465 . . 462 465 + TspEI AATT 461 465 . . 470 475 + Hpy178III* TCNNGA 471 473 . . 476 482 + TspRI CASTGNN 482 473 . . 483 486 + SetI ASST 486 482 . . 499 502 + MaeI CTAG 499 501 . . 503 506 + SetI ASST 506 502 . . 502 505 - MnlI CCTC 495 494 . . 508 512 + TfiI GAWTC 508 511 . . 508 512 + HinfI GANTC 508 511 . . 502 512 - Hin4I GAYNNNNNVTC 525 520 493 488 514 518 + Hpy188I TCNGA 516 515 . . 511 515 - MboII GAAGA 503 502 . . 524 527 + TaqI TCGA 524 526 . . 529 534 + AclI AACGTT 530 532 . . 530 533 + TaiI ACGT 533 529 . . 530 533 + SetI ASST 533 529 . . 530 533 + MaeII ACGT 530 532 . . 533 536 + MseI TTAA 533 535 . . 538 541 + TaqI TCGA 538 540 . . 542 547 + ApoI RAATTY 542 546 . . 543 546 + TspEI AATT 542 546 . . 546 551 + Hpy178III* TCNNGA 547 549 . . 556 565 + XmnI GAANNNNTTC 560 560 . . 558 561 + SetI ASST 561 557 . . 556 560 - Hin4II* CCTTC 550 549 . . 565 568 + MnlI CCTC 575 574 . . 567 572 + Hpy178III* TCNNGA 568 570 . . 566 571 - Eco57MI CTGRAG 551 549 . . 571 574 + DpnI GATC 572 572 . . 571 574 + BstKTI GATC 573 571 . . 566 571 - GsuI CTGGAG 551 549 . . 571 574 + MboI GATC 570 574 . . 603 607 + BsrI ACTGG 608 606 . . 607 611 + MboII GAAGA 619 618 . . 607 612 + BbvII* GAAGAC 614 618 . . 611 615 + Tsp4CI* ACNGT 613 612 . . 625 629 + Hpy188I TCNGA 627 626 . . 630 635 + PsiI TTATAA 632 632 . . 639 643 + Hpy188I TCNGA 641 640 . . 646 650 + PleI GAGTC 654 655 . . 646 650 + MlyI GAGTC 655 655 . . 646 650 + HinfI GANTC 646 649 . . 648 653 + Hpy166II GTNNAC 650 650 . . 648 653 + AccI GTMKAC 649 651 . . 652 655 + TaiI ACGT 655 651 . . 652 655 + SetI ASST 655 651 . . 652 655 + MaeII ACGT 652 654 . . 656 659 + CviRI* TGCA 657 657 . . 678 681 + SetI ASST 681 677 . . 677 680 - MnlI CCTC 670 669 . . 686 689 + CviRI* TGCA 687 687 . . 689 694 + ApoI RAATTY 689 693 . . 690 693 + TspEI AATT 689 693 . . 714 718 + MboII GAAGA 726 725 . . 714 719 + BbvII* GAAGAC 721 725 . . 727 730 + MaeI CTAG 727 729 . . 731 734 + SetI ASST 734 730 . . 730 733 - MnlI CCTC 723 722 . . 736 740 + TfiI GAWTC 736 739 . . 736 740 + HinfI GANTC 736 739 . . 730 740 - Hin4I GAYNNNNNVTC 753 748 721 716 742 747 + MmeI TCCRAC 767 765 . . 742 746 + Hpy188I TCNGA 744 743 . . 739 743 - MboII GAAGA 731 730 . . 754 762 + Tth111I GACNNNGTC 757 758 . . 758 761 + TaiI ACGT 761 757 . . 758 761 + SetI ASST 761 757 . . 758 761 + MaeII ACGT 758 760 . . 770 775 + ApoI RAATTY 770 774 . . 771 774 + TspEI AATT 770 774 . . 774 779 + Hpy178III* TCNNGA 775 777 . . 786 789 + SetI ASST 789 785 . . 784 788 - Hin4II* CCTTC 778 777 . . 788 793 + BciVI GTATCC 799 798 . . 793 796 + MnlI CCTC 803 802 . . 795 800 + Hpy178III* TCNNGA 796 798 . . 795 800 + BetI* WCCGGW 795 799 . . 795 800 + BspMII* TCCGGA 795 799 . . 796 799 + HpaII CCGG 796 798 . . 798 802 + BinI* GGATC 806 807 . . 799 802 + DpnI GATC 800 800 . . 799 802 + BstKTI GATC 801 799 . . 799 802 + MboI GATC 798 802 . . 832 835 + MaeI CTAG 832 834 . . 835 839 + MboII GAAGA 847 846 . . 838 842 - BccI CCATC 832 833 . . 847 850 + SetI ASST 850 846 . . 853 857 + Hpy188I TCNGA 855 854 . . 864 868 - FokI GGATG 850 854 . . 864 868 - BseGI GGATG 863 861 . . 874 878 + TfiI GAWTC 874 877 . . 874 878 + HinfI GANTC 874 877 . . 881 886 + Ksp632I* CTCTTC 887 890 . . 882 886 - MboII GAAGA 874 873 . . 886 894 + DraIII CACNNNGTG 891 888 . . 901 905 + DdeI CTNAG 901 904 . . 897 901 - BsmAI GTCTC 891 895 . . 901 905 - BspCNI CTCAG 893 891 . . 901 905 - BseMII CTCAG 892 890 . . 906 909 + SetI ASST 909 905 . . 905 908 - MnlI CCTC 898 897 . . 914 917 + CviRI* TGCA 915 915 . . 917 922 + ApoI RAATTY 917 921 . . 918 921 + TspEI AATT 917 921 . . 926 931 + Hpy178III* TCNNGA 927 929 . . 932 938 + TspRI CASTGNN 938 929 . . 939 942 + SetI ASST 942 938 . . 955 958 + MaeI CTAG 955 957 . . 959 962 + SetI ASST 962 958 . . 958 961 - MnlI CCTC 951 950 . . 964 968 + TfiI GAWTC 964 967 . . 964 968 + HinfI GANTC 964 967 . . 958 968 - Hin4I GAYNNNNNVTC 981 976 949 944 970 974 + Hpy188I TCNGA 972 971 . . 967 971 - MboII GAAGA 959 958 . . 985 990 + AclI AACGTT 986 988 . . 986 989 + TaiI ACGT 989 985 . . 986 989 + SetI ASST 989 985 . . 986 989 + MaeII ACGT 986 988 . . 989 992 + MseI TTAA 989 991 . . 994 997 + TaqI TCGA 994 996 . . 998 1003 + ApoI RAATTY 998 1002 . . 999 1002 + TspEI AATT 998 1002 . . 1002 1007 + Hpy178III* TCNNGA 1003 1005 . . 1012 1021 + XmnI GAANNNNTTC 1016 1016 . . 1014 1017 + SetI ASST 1017 1013 . . 1012 1016 - Hin4II* CCTTC 1006 1005 . . 1021 1024 + MnlI CCTC 1031 1030 . . 1023 1028 + Hpy178III* TCNNGA 1024 1026 . . 1022 1027 - Eco57MI CTGRAG 1007 1005 . . 1022 1027 - GsuI CTGGAG 1007 1005 . . 1048 1053 + Cfr10I RCCGGY 1048 1052 . . 1049 1052 + HpaII CCGG 1049 1051 . . 1060 1063 + MaeI CTAG 1060 1062 . . 1063 1067 + MboII GAAGA 1075 1074 . . 1066 1070 - BccI CCATC 1060 1061 . . 1081 1085 + Hpy188I TCNGA 1083 1082 . . 1080 1085 - MmeI TCCRAC 1061 1059 . . 1090 1095 + SspI AATATT 1092 1092 . . 1102 1106 + PleI GAGTC 1110 1111 . . 1102 1106 + MlyI GAGTC 1111 1111 . . 1102 1106 + HinfI GANTC 1102 1105 . . 1104 1109 + Hpy166II GTNNAC 1106 1106 . . 1109 1114 + Ksp632I* CTCTTC 1115 1118 . . 1110 1114 - MboII GAAGA 1102 1101 . . 1115 1118 + SetI ASST 1118 1114 . . 1113 1117 - HphI GGTGA 1105 1104 . . 1121 1126 + Hpy178III* TCNNGA 1122 1124 . . 1122 1127 + SmlI CTYRAG 1122 1126 . . 1122 1127 + Bce83I* CTTGAG 1143 1141 . . 1126 1129 + SetI ASST 1129 1125 . . 1125 1128 - MnlI CCTC 1118 1117 . . 1131 1134 - MnlI CCTC 1124 1123 . . 1139 1143 + MaeIII GTNAC 1138 1143 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 497 649 # # AclI: # [AACGTT] # 74 160 228 228 456 # # AgeI: # [ACCGGT] # 25 228 893 # # AluI: # [AGCT] # 147 228 771 # # ApoI: # [RAATTY] # 81 81 81 86 147 147 # 148 375 # # BbvI: # [GCAGC] # 156 228 762 # # BbvII*: # [GAAGAC] # 107 158 425 456 # # BccI: # [CCATC] # 73 86 105 198 228 456 # # Bce83I*: # [CTTGAG] # 3 1143 # # BciVI: # [GTATCC] # 115 228 347 456 # # BetI*: # [WCCGGW] # 25 86 228 351 456 # # BglII: # [AGATCT] # 233 913 # # BinI*: # [GGATC] # 340 350 456 # # BisI: # [GCNGC] # 145 228 773 # # BlsI: # [GCNGC] # 146 228 772 # # Bpu10I: # [CCTNAGC] # 211 935 # # BseBI: # [CCWGG] # 319 827 # # BseGI: # [GGATG] # 283 407 456 # # BseMII: # [CTCAG] # 254 436 456 # # BsiYI*: # [CCNNNNNNNGG] # 324 822 # # BsmAI: # [GTCTC] # 255 435 456 # # BspCNI: # [CTCAG] # 253 437 456 # # BspMII*: # [TCCGGA] # 339 351 456 # # BsrI: # [ACTGG] # 538 608 # # BssKI: # [CCNGG] # 317 829 # # BstKTI: # [GATC] # 14 14 49 60 105 117 # 214 228 345 # # BstXI: # [CCANNNNNNTGG] # 42 1104 # # Cfr10I: # [RCCGGY] # 25 98 111 111 117 684 # # ClaI: # [ATCGAT] # 296 850 # # CviJI: # [RGCY] # 69 147 159 771 # # CviRI*: # [TGCA] # 3 30 198 228 228 228 # 231 # # DdeI: # [CTNAG] # 6 211 228 245 456 # # DpnI: # [GATC] # 14 14 49 60 105 116 # 214 228 346 # # DraIII: # [CACNNNGTG] # 255 435 456 # # Eco57MI: # [CTGRAG] # 95 139 456 456 # # EcoRII: # [CCWGG] # 317 829 # # FokI: # [GGATG] # 296 394 456 # # GsuI: # [CTGGAG] # 95 139 456 456 # # Hin4I: # [GAYNNNNNVTC] # 32 32 32 32 165 196 # 196 196 265 # # Hin4II*: # [CCTTC] # 86 94 140 142 228 228 # 228 # # HinfI: # [GANTC] # 44 52 90 90 90 90 # 138 138 138 138 138 # # HpaII: # [CCGG] # 25 26 86 97 111 117 # 253 431 # # HphI: # [GGTGA] # 41 1105 # # Hpy166II: # [GTNNAC] # 40 194 456 456 # # Hpy178III*: # [TCNNGA] # 15 21 21 21 21 24 # 76 76 76 98 131 131 # 207 228 # # Hpy188I: # [TCNGA] # 14 14 60 63 103 103 # 111 111 111 111 111 117 # 117 # # Ksp632I*: # [CTCTTC] # 31 228 431 456 # # MaeI: # [CTAG] # 86 105 105 123 123 148 # 228 228 # # MaeII: # [ACGT] # 74 106 122 160 228 228 # 228 # # MaeIII: # [GTNAC] # 8 1138 # # MboI: # [GATC] # 14 14 49 60 105 114 # 214 228 348 # # MboII: # [GAAGA] # 5 27 27 27 44 47 # 85 85 107 116 116 116 # 116 228 # # McrI*: # [CGRYCG] # 296 850 # # MlyI: # [GAGTC] # 35 199 456 456 # # MmeI: # [TCCRAC] # 85 294 311 456 # # MnlI: # [CCTC] # 3 6 22 27 50 53 # 53 53 53 80 80 80 # 87 95 95 95 95 119 # # MseI: # [TTAA] # 63 77 157 165 228 456 # # PfoI: # [TCCNGGA] # 317 829 # # PleI: # [GAGTC] # 36 198 456 456 # # PsiI: # [TTATAA] # 514 632 # # PvuI: # [CGATCG] # 296 850 # # ScrFI: # [CCNGG] # 319 827 # # SetI: # [ASST] # 11 17 17 20 20 22 # 22 26 26 27 27 27 # 27 28 28 28 28 28 # 33 33 44 50 53 53 # 59 59 61 77 94 101 # # SmlI: # [CTYRAG] # 24 1122 # # SspI: # [AATATT] # 54 1092 # # TaiI: # [ACGT] # 77 106 122 157 228 228 # 228 # # TaqI: # [TCGA] # 14 14 14 68 152 214 # 214 456 # # TfiI: # [GAWTC] # 52 90 90 138 138 182 # 228 228 # # TseI: # [GCWGC] # 144 228 774 # # Tsp4CI*: # [ACNGT] # 157 456 533 # # TspEI: # [AATT] # 81 81 81 86 147 147 # 148 375 # # TspRI: # [CASTGNN] # 208 456 482 # # Tth111I: # [GACNNNGTC] # 389 757 # # XhoII: # [RGATCY] # 233 913 # # XmnI: # [GAANNNNTTC] # 130 456 560 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1146 # Reported_sequences: 1 # Reported_hitcount: 301 #---------------------------------------