Restriction Fragments for YKRCdelta11



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The currently selected gene/sequence is :

YKRCdelta11 on chromosome XI from coordinates 513846 to 513532.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25329.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 21 26 + SspI AATATT 23 23 . . 25 29 - TspDTI ATGAA 15 13 . . 34 37 + MaeI CTAG 34 36 . . 37 42 + Hpy166II GTNNAC 39 39 . . 37 42 + BssNAI GTATAC 39 39 . . 37 42 + AccI GTMKAC 38 40 . . 46 51 + NdeI CATATG 47 49 . . 51 54 - AciI CCGC 51 53 . . 60 64 + MboII GAAGA 72 71 . . 60 65 - Ksp632I* CTCTTC 55 58 . . 63 66 - MnlI CCTC 56 55 . . 72 75 + SetI ASST 75 71 . . 97 101 + TspDTI ATGAA 112 110 . . 102 105 + SetI ASST 105 101 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + AluI AGCT 103 103 . . 110 113 + CviRI* TGCA 111 111 . . 137 141 + TspDTI ATGAA 152 150 . . 191 196 + ClaI ATCGAT 192 194 . . 192 195 + TaqI TCGA 192 194 . . 194 198 + TfiI GAWTC 194 197 . . 194 198 + HinfI GANTC 194 197 . . 198 208 + BsiYI* CCNNNNNNNGG 204 201 . . 208 212 + TfiI GAWTC 208 211 . . 205 208 - MnlI CCTC 198 197 . . 208 212 + HinfI GANTC 208 211 . . 217 222 + SduI GDGCHC 221 217 . . 217 222 + HgiAI* GWGCWC 221 217 . . 219 226 + PspXI VCTCGAGB 220 224 . . 220 225 + SmlI CTYRAG 220 224 . . 220 225 + XhoI CTCGAG 220 224 . . 220 225 + BmeT110I CYCGRG 221 223 . . 220 225 + AvaI CYCGRG 220 224 . . 221 224 + TaqI TCGA 221 223 . . 224 229 + TatI WGTACW 224 228 . . 225 228 + Csp6I GTAC 225 227 . . 225 228 + RsaI GTAC 226 226 . . 229 232 + TspEI AATT 228 232 . . 234 237 + MaeI CTAG 234 236 . . 278 282 + SfaNI GCATC 287 291 . . 279 283 - FokI GGATG 265 269 . . 279 283 - BseGI GGATG 278 276 . . 288 291 + TspEI AATT 287 291 . . 295 298 + MseI TTAA 295 297 . . 298 303 + ApoI RAATTY 298 302 . . 299 302 + TspEI AATT 298 302 . . 302 306 - HphI GGTGA 294 293 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 38 277 # # AciI: # [CCGC] # 51 264 # # AluI: # [AGCT] # 103 212 # # ApoI: # [RAATTY] # 17 298 # # AvaI: # [CYCGRG] # 95 220 # # BmeT110I: # [CYCGRG] # 94 221 # # BseGI: # [GGATG] # 37 278 # # BsiYI*: # [CCNNNNNNNGG] # 111 204 # # BssNAI: # [GTATAC] # 39 276 # # ClaI: # [ATCGAT] # 123 192 # # Csp6I: # [GTAC] # 90 225 # # CviJI: # [RGCY] # 103 212 # # CviRI*: # [TGCA] # 111 204 # # FokI: # [GGATG] # 50 265 # # HgiAI*: # [GWGCWC] # 94 221 # # HinfI: # [GANTC] # 14 107 194 # # HphI: # [GGTGA] # 21 294 # # Hpy166II: # [GTNNAC] # 39 276 # # Ksp632I*: # [CTCTTC] # 55 260 # # MaeI: # [CTAG] # 34 81 200 # # MboII: # [GAAGA] # 72 243 # # MnlI: # [CCTC] # 56 117 142 # # MseI: # [TTAA] # 20 295 # # NdeI: # [CATATG] # 47 268 # # PspXI: # [VCTCGAGB] # 95 220 # # RsaI: # [GTAC] # 89 226 # # SduI: # [GDGCHC] # 94 221 # # SetI: # [ASST] # 30 75 210 # # SfaNI: # [GCATC] # 28 287 # # SmlI: # [CTYRAG] # 95 220 # # SspI: # [AATATT] # 23 292 # # TaqI: # [TCGA] # 29 94 192 # # TatI: # [WGTACW] # 91 224 # # TfiI: # [GAWTC] # 14 107 194 # # TspDTI: # [ATGAA] # 15 40 97 163 # # TspEI: # [AATT] # 11 17 59 228 # # XhoI: # [CTCGAG] # 95 220 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 315 # Reported_sequences: 1 # Reported_hitcount: 47 #---------------------------------------