Restriction Fragments for FLO10/YKR102W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

FLO10/YKR102W on chromosome XI from coordinates 646356 to 649865.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2026.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 12 + CviJI RGCY 10 10 . . 10 14 + BlsI GCNGC 12 11 . . 10 14 + BisI GCNGC 11 12 . . 10 14 + TseI GCWGC 10 13 . . 15 18 + TaqI TCGA 15 17 . . 31 36 + Cfr10I RCCGGY 31 35 . . 32 35 + HpaII CCGG 32 34 . . 34 37 + CviJI RGCY 35 35 . . 34 37 + HaeIII GGCC 35 35 . . 50 55 + SfeI* CTRYAG 50 54 . . 54 57 + SetI ASST 57 53 . . 54 57 + CviJI RGCY 55 55 . . 54 57 + AluI AGCT 55 55 . . 55 65 + MwoI GCNNNNNNNGC 61 58 . . 67 70 + MaeI CTAG 67 69 . . 69 72 + SetI ASST 72 68 . . 71 74 + Csp6I GTAC 71 73 . . 71 74 + RsaI GTAC 72 72 . . 74 79 + SfeI* CTRYAG 74 78 . . 81 84 + CviJI RGCY 82 82 . . 79 82 - MnlI CCTC 72 71 . . 90 95 + Cac8I GCNNGC 92 92 . . 92 97 + PvuII CAGCTG 94 94 . . 92 97 + NspBII* CMGCKG 94 94 . . 93 96 + SetI ASST 96 92 . . 93 96 + CviJI RGCY 94 94 . . 93 96 + AluI AGCT 94 94 . . 95 100 + Eco57MI CTGRAG 116 114 . . 95 100 + GsuI CTGGAG 116 114 . . 102 106 + MboII GAAGA 114 113 . . 134 139 + SspI AATATT 136 136 . . 142 145 + MseI TTAA 142 144 . . 148 152 + TfiI GAWTC 148 151 . . 148 152 + HinfI GANTC 148 151 . . 150 154 - TspDTI ATGAA 140 138 . . 168 172 - TspGWI ACGGA 158 156 . . 180 183 + CviJI RGCY 181 181 . . 180 183 + HaeIII GGCC 181 181 . . 186 191 - TsoI TARCCA 176 174 . . 198 201 + CviRI* TGCA 199 199 . . 196 200 - SfaNI GCATC 186 190 . . 207 211 + BsrI ACTGG 212 210 . . 207 212 + BfiI ACTGGG 217 216 . . 225 235 + MwoI GCNNNNNNNGC 231 228 . . 232 237 + HindIII AAGCTT 232 236 . . 233 236 + SetI ASST 236 232 . . 233 236 + CviJI RGCY 234 234 . . 233 236 + AluI AGCT 234 234 . . 239 243 + BccI CCATC 247 248 . . 241 246 + ClaI ATCGAT 242 244 . . 242 245 + TaqI TCGA 242 244 . . 251 255 + BccI CCATC 259 260 . . 252 256 - FokI GGATG 238 242 . . 252 256 - BseGI GGATG 251 249 . . 256 266 + BsiYI* CCNNNNNNNGG 262 259 . . 256 266 + PflMI CCANNNNNTGG 262 259 . . 257 260 + FatI CATG 256 260 . . 257 260 + NlaIII CATG 260 256 . . 257 260 + CviAII CATG 257 259 . . 266 269 + Hin6I GCGC 266 268 . . 266 269 + GlaI GCGC 267 267 . . 266 269 + HhaI GCGC 268 266 . . 268 272 + SfaNI GCATC 277 281 . . 271 275 + Hpy188I TCNGA 273 272 . . 289 293 + Hpy188I TCNGA 291 290 . . 291 302 + BcgI CGANNNNNNTGC 280 278 314 312 298 302 - SfaNI GCATC 288 292 . . 313 318 + HaeII RGCGCY 317 313 . . 313 318 + Eco47III AGCGCT 315 315 . . 314 317 + Hin6I GCGC 314 316 . . 314 317 + GlaI GCGC 315 315 . . 314 317 + HhaI GCGC 316 314 . . 320 325 + SecI* CCNNGG 320 324 . . 321 325 + BssKI CCNGG 320 325 . . 321 325 + BseBI CCWGG 322 323 . . 321 325 + ScrFI CCNGG 322 323 . . 321 325 + EcoRII CCWGG 320 325 . . 323 326 + SetI ASST 326 322 . . 328 331 + Csp6I GTAC 328 330 . . 328 331 + RsaI GTAC 329 329 . . 332 338 + TspRI CASTGNN 338 329 . . 331 335 - BsrI ACTGG 331 329 . . 347 350 + MseI TTAA 347 349 . . 358 362 + Hpy188I TCNGA 360 359 . . 376 380 - MboII GAAGA 368 367 . . 386 390 + DdeI CTNAG 386 389 . . 386 390 - BspCNI CTCAG 378 376 . . 386 390 - BseMII CTCAG 377 375 . . 393 396 + TaiI ACGT 396 392 . . 393 396 + SetI ASST 396 392 . . 393 396 + MaeII ACGT 393 395 . . 406 415 + BsaBI GATNNNNATC 410 410 . . 410 420 + MwoI GCNNNNNNNGC 416 413 . . 412 415 + DpnI GATC 413 413 . . 412 415 + BstKTI GATC 414 412 . . 412 415 + MboI GATC 411 415 . . 418 423 + HaeII RGCGCY 422 418 . . 419 422 + Hin6I GCGC 419 421 . . 419 422 + GlaI GCGC 420 420 . . 419 422 + HhaI GCGC 421 419 . . 421 425 + BlsI GCNGC 423 422 . . 421 425 + BisI GCNGC 422 423 . . 421 425 + TseI GCWGC 421 424 . . 421 425 - BbvI GCAGC 408 412 . . 428 432 + BsrI ACTGG 433 431 . . 429 434 + Eco57MI CTGRAG 450 448 . . 429 434 + GsuI CTGGAG 450 448 . . 436 440 + Hpy188I TCNGA 438 437 . . 438 443 + XhoII RGATCY 438 442 . . 438 443 + BglII AGATCT 438 442 . . 439 442 + DpnI GATC 440 440 . . 439 442 + BstKTI GATC 441 439 . . 439 442 + MboI GATC 438 442 . . 432 442 - Hin4I GAYNNNNNVTC 455 450 423 418 446 450 + Hpy188I TCNGA 448 447 . . 449 453 + TfiI GAWTC 449 452 . . 449 453 + HinfI GANTC 449 452 . . 461 466 + TaqII CACCCA 477 475 . . 467 481 + XcmI CCANNNNNNNNNTGG 474 473 . . 470 473 + TaiI ACGT 473 469 . . 470 473 + SetI ASST 473 469 . . 470 473 + MaeII ACGT 470 472 . . 472 476 + MaeIII GTNAC 471 476 . . 475 479 + Tsp4CI* ACNGT 477 476 . . 476 481 + DsaI* CCRYGG 476 480 . . 476 481 + SecI* CCNNGG 476 480 . . 489 492 + SetI ASST 492 488 . . 491 495 + MaeIII GTNAC 490 495 . . 505 515 + BsiYI* CCNNNNNNNGG 511 508 . . 505 515 + PflMI CCANNNNNTGG 511 508 . . 511 515 + BsrI ACTGG 516 514 . . 514 519 + KpnI GGTACC 518 514 . . 514 519 + NlaIV GGNNCC 516 516 . . 514 519 + HgiCI* GGYRCC 514 518 . . 514 519 + Acc65I GGTACC 514 518 . . 515 518 + Csp6I GTAC 515 517 . . 515 518 + RsaI GTAC 516 516 . . 517 520 + SetI ASST 520 516 . . 529 532 + CviJI RGCY 530 530 . . 538 542 + Tsp4CI* ACNGT 540 539 . . 543 547 + FokI GGATG 556 560 . . 543 547 + BseGI GGATG 549 547 . . 547 551 + TfiI GAWTC 547 550 . . 547 551 + HinfI GANTC 547 550 . . 555 558 + TspEI AATT 554 558 . . 553 564 - BcgI CGANNNNNNTGC 576 574 542 540 570 573 + SetI ASST 573 569 . . 565 570 - MmeI TCCRAC 546 544 . . 569 572 - MnlI CCTC 562 561 . . 568 578 - BsaXI ACNNNNNCTCC 590 587 560 557 593 598 + Hpy166II GTNNAC 595 595 . . 605 608 + CviJI RGCY 606 606 . . 607 610 + MnlI CCTC 617 616 . . 622 626 + TspGWI ACGGA 637 635 . . 635 638 + MseI TTAA 635 637 . . 638 642 + Tsp4CI* ACNGT 640 639 . . 644 647 + MseI TTAA 644 646 . . 649 654 + DsaI* CCRYGG 649 653 . . 649 654 + StyI CCWWGG 649 653 . . 649 654 + NcoI CCATGG 649 653 . . 649 654 + SecI* CCNNGG 649 653 . . 650 653 + FatI CATG 649 653 . . 650 653 + NlaIII CATG 653 649 . . 650 653 + CviAII CATG 650 652 . . 663 666 + CviRI* TGCA 664 664 . . 661 665 - SfaNI GCATC 651 655 . . 666 669 + SetI ASST 669 665 . . 684 689 + Hpy166II GTNNAC 686 686 . . 684 689 + HindII GTYRAC 686 686 . . 688 691 + TaiI ACGT 691 687 . . 688 691 + SetI ASST 691 687 . . 688 691 + MaeII ACGT 688 690 . . 690 693 + Csp6I GTAC 690 692 . . 690 693 + RsaI GTAC 691 691 . . 692 697 + BspLU11I* ACATGT 692 696 . . 692 697 + NspI RCATGY 696 692 . . 692 697 + AflIII ACRYGT 692 696 . . 693 696 + FatI CATG 692 696 . . 693 696 + NlaIII CATG 696 692 . . 693 696 + CviAII CATG 693 695 . . 696 699 + Csp6I GTAC 696 698 . . 696 699 + RsaI GTAC 697 697 . . 700 705 + Cfr10I RCCGGY 700 704 . . 701 704 + HpaII CCGG 701 703 . . 704 708 + MaeIII GTNAC 703 708 . . 717 720 + DpnI GATC 718 718 . . 717 720 + BstKTI GATC 719 717 . . 717 720 + MboI GATC 716 720 . . 723 726 + TspEI AATT 722 726 . . 742 747 + BciVI GTATCC 753 752 . . 746 750 + BssKI CCNGG 745 750 . . 746 750 + BseBI CCWGG 747 748 . . 746 751 + SecI* CCNNGG 746 750 . . 746 750 + ScrFI CCNGG 747 748 . . 746 750 + EcoRII CCWGG 745 750 . . 752 755 + Csp6I GTAC 752 754 . . 752 755 + RsaI GTAC 753 753 . . 785 788 + Csp6I GTAC 785 787 . . 781 785 - BccI CCATC 775 776 . . 785 788 + RsaI GTAC 786 786 . . 788 792 + DdeI CTNAG 788 791 . . 791 794 + SetI ASST 794 790 . . 788 792 - BspCNI CTCAG 780 778 . . 788 792 - BseMII CTCAG 779 777 . . 790 793 - MnlI CCTC 783 782 . . 794 797 + MseI TTAA 794 796 . . 784 794 - BaeI ACNNNNGTAYC 809 804 776 771 808 812 - Hin4II* CCTTC 802 801 . . 838 848 + MwoI GCNNNNNNNGC 844 841 . . 842 847 + SmlI CTYRAG 842 846 . . 846 849 + SetI ASST 849 845 . . 846 849 + CviJI RGCY 847 847 . . 846 849 + AluI AGCT 847 847 . . 842 847 - Bce83I* CTTGAG 827 825 . . 850 856 + TspRI CASTGNN 856 847 . . 851 855 + Tsp4CI* ACNGT 853 852 . . 856 860 - TspGWI ACGGA 846 844 . . 871 881 + MwoI GCNNNNNNNGC 877 874 . . 871 877 + EspI* GCTNAGC 872 875 . . 872 876 + DdeI CTNAG 872 875 . . 869 874 - EcoP15I CAGCAG 841 843 . . 876 881 + NspI RCATGY 880 876 . . 876 881 + Cac8I GCNNGC 878 878 . . 872 876 - BspCNI CTCAG 864 862 . . 872 876 - BseMII CTCAG 863 861 . . 876 881 + SphI GCATGC 880 876 . . 877 880 + FatI CATG 876 880 . . 877 880 + NlaIII CATG 880 876 . . 877 880 + CviAII CATG 877 879 . . 879 882 + CviRI* TGCA 880 880 . . 886 896 + BaeI ACNNNNGTAYC 875 870 908 903 900 903 + CviRI* TGCA 901 901 . . 907 911 - MboII GAAGA 899 898 . . 911 915 + BssKI CCNGG 910 915 . . 911 915 + BseBI CCWGG 912 913 . . 911 915 + ScrFI CCNGG 912 913 . . 911 915 + EcoRII CCWGG 910 915 . . 921 927 + TspRI CASTGNN 927 918 . . 920 924 - BsrI ACTGG 920 918 . . 931 936 + TatI WGTACW 931 935 . . 931 936 + Bsp1407I TGTACA 931 935 . . 932 935 + Csp6I GTAC 932 934 . . 932 935 + RsaI GTAC 933 933 . . 939 944 + Hpy166II GTNNAC 941 941 . . 941 944 + Csp6I GTAC 941 943 . . 941 944 + RsaI GTAC 942 942 . . 948 953 + TaqII GACCGA 964 962 . . 948 956 + Tth111I GACNNNGTC 951 952 . . 946 950 - BsmAI GTCTC 940 944 . . 946 951 - Eco31I GGTCTC 940 944 . . 952 956 + PleI GAGTC 960 961 . . 952 956 + MlyI GAGTC 961 961 . . 952 956 + HinfI GANTC 952 955 . . 954 959 + Hpy166II GTNNAC 956 956 . . 954 959 + AccI GTMKAC 955 957 . . 946 956 - Hin4I GAYNNNNNVTC 969 964 937 932 959 963 - BsrI ACTGG 959 957 . . 967 971 + Tsp45I GTSAC 966 971 . . 967 971 + MaeIII GTNAC 966 971 . . 974 979 + NdeI CATATG 975 977 . . 979 983 + Tsp45I GTSAC 978 983 . . 979 983 + MaeIII GTNAC 978 983 . . 983 986 + MaeI CTAG 983 985 . . 985 988 + SetI ASST 988 984 . . 985 988 + CviJI RGCY 986 986 . . 985 988 + AluI AGCT 986 986 . . 986 992 + SapI GCTCTTC 993 996 . . 987 992 + Ksp632I* CTCTTC 993 996 . . 988 992 - MboII GAAGA 980 979 . . 992 996 + BssKI CCNGG 991 996 . . 992 996 + BseBI CCWGG 993 994 . . 992 996 + ScrFI CCNGG 993 994 . . 992 996 + EcoRII CCWGG 991 996 . . 1002 1008 + TspRI CASTGNN 1008 999 . . 1001 1005 - BsrI ACTGG 1001 999 . . 1012 1017 + TatI WGTACW 1012 1016 . . 1012 1017 + Bsp1407I TGTACA 1012 1016 . . 1013 1016 + Csp6I GTAC 1013 1015 . . 1013 1016 + RsaI GTAC 1014 1014 . . 1020 1025 + Hpy166II GTNNAC 1022 1022 . . 1020 1025 + SduI GDGCHC 1024 1020 . . 1020 1025 + BseSI GKGCMC 1024 1020 . . 1020 1025 + ApaLI GTGCAC 1020 1024 . . 1020 1025 + HgiAI* GWGCWC 1024 1020 . . 1021 1024 + CviRI* TGCA 1022 1022 . . 1029 1034 + TaqII GACCGA 1045 1043 . . 1029 1037 + Tth111I GACNNNGTC 1032 1033 . . 1027 1031 - BsmAI GTCTC 1021 1025 . . 1027 1032 - Eco31I GGTCTC 1021 1025 . . 1033 1037 + PleI GAGTC 1041 1042 . . 1033 1037 + MlyI GAGTC 1042 1042 . . 1033 1037 + HinfI GANTC 1033 1036 . . 1035 1040 + Hpy166II GTNNAC 1037 1037 . . 1035 1040 + AccI GTMKAC 1036 1038 . . 1027 1037 - Hin4I GAYNNNNNVTC 1050 1045 1018 1013 1040 1043 + MaeI CTAG 1040 1042 . . 1044 1055 + TstI CACNNNNNNTCC 1035 1030 1067 1062 1045 1048 + SetI ASST 1048 1044 . . 1045 1055 + BsaXI ACNNNNNCTCC 1035 1032 1065 1062 1046 1049 + MnlI CCTC 1056 1055 . . 1055 1060 + NdeI CATATG 1056 1058 . . 1060 1064 + Tsp45I GTSAC 1059 1064 . . 1060 1064 + MaeIII GTNAC 1059 1064 . . 1061 1065 - HphI GGTGA 1053 1052 . . 1066 1069 + SetI ASST 1069 1065 . . 1066 1069 + CviJI RGCY 1067 1067 . . 1066 1069 + AluI AGCT 1067 1067 . . 1067 1073 + SapI GCTCTTC 1074 1077 . . 1068 1073 + Ksp632I* CTCTTC 1074 1077 . . 1073 1076 + MnlI CCTC 1083 1082 . . 1069 1073 - MboII GAAGA 1061 1060 . . 1076 1080 + Hpy99I CGWCG 1080 1075 . . 1083 1089 + TspRI CASTGNN 1089 1080 . . 1082 1086 - BsrI ACTGG 1082 1080 . . 1094 1097 + Csp6I GTAC 1094 1096 . . 1094 1097 + RsaI GTAC 1095 1095 . . 1096 1100 + TspGWI ACGGA 1111 1109 . . 1101 1106 + Hpy166II GTNNAC 1103 1103 . . 1101 1106 + SduI GDGCHC 1105 1101 . . 1101 1106 + BseSI GKGCMC 1105 1101 . . 1101 1106 + ApaLI GTGCAC 1101 1105 . . 1101 1106 + HgiAI* GWGCWC 1105 1101 . . 1102 1105 + CviRI* TGCA 1103 1103 . . 1112 1116 + DdeI CTNAG 1112 1115 . . 1114 1118 + PleI GAGTC 1122 1123 . . 1114 1118 + MlyI GAGTC 1123 1123 . . 1114 1118 + HinfI GANTC 1114 1117 . . 1116 1121 + Hpy166II GTNNAC 1118 1118 . . 1112 1116 - BspCNI CTCAG 1104 1102 . . 1112 1116 - BseMII CTCAG 1103 1101 . . 1116 1121 + AccI GTMKAC 1117 1119 . . 1121 1125 - BsrI ACTGG 1121 1119 . . 1129 1133 + Tsp45I GTSAC 1128 1133 . . 1129 1133 + MaeIII GTNAC 1128 1133 . . 1136 1141 + NdeI CATATG 1137 1139 . . 1144 1147 + SetI ASST 1147 1143 . . 1144 1147 + CviJI RGCY 1145 1145 . . 1144 1147 + AluI AGCT 1145 1145 . . 1149 1154 + Hpy166II GTNNAC 1151 1151 . . 1149 1154 + AccI GTMKAC 1150 1152 . . 1156 1160 + BlsI GCNGC 1158 1157 . . 1156 1160 + BisI GCNGC 1157 1158 . . 1156 1160 + TseI GCWGC 1156 1159 . . 1159 1163 + TauI GCSGC 1162 1159 . . 1154 1159 - EcoP15I CAGCAG 1126 1128 . . 1159 1163 + BlsI GCNGC 1161 1160 . . 1159 1163 + BisI GCNGC 1160 1161 . . 1156 1160 - BbvI GCAGC 1143 1147 . . 1160 1163 + AciI CCGC 1160 1162 . . 1172 1175 + MaeI CTAG 1172 1174 . . 1174 1177 + SetI ASST 1177 1173 . . 1174 1177 + CviJI RGCY 1175 1175 . . 1174 1177 + AluI AGCT 1175 1175 . . 1191 1197 + TspRI CASTGNN 1197 1188 . . 1190 1194 - BsrI ACTGG 1190 1188 . . 1201 1206 + TatI WGTACW 1201 1205 . . 1201 1206 + Bsp1407I TGTACA 1201 1205 . . 1202 1205 + Csp6I GTAC 1202 1204 . . 1202 1205 + RsaI GTAC 1203 1203 . . 1209 1214 + Hpy166II GTNNAC 1211 1211 . . 1209 1214 + SduI GDGCHC 1213 1209 . . 1209 1214 + BseSI GKGCMC 1213 1209 . . 1209 1214 + ApaLI GTGCAC 1209 1213 . . 1209 1214 + HgiAI* GWGCWC 1213 1209 . . 1210 1213 + CviRI* TGCA 1211 1211 . . 1220 1224 + DdeI CTNAG 1220 1223 . . 1222 1226 + PleI GAGTC 1230 1231 . . 1222 1226 + MlyI GAGTC 1231 1231 . . 1222 1226 + HinfI GANTC 1222 1225 . . 1224 1229 + Hpy166II GTNNAC 1226 1226 . . 1220 1224 - BspCNI CTCAG 1212 1210 . . 1220 1224 - BseMII CTCAG 1211 1209 . . 1224 1229 + AccI GTMKAC 1225 1227 . . 1229 1232 + MaeI CTAG 1229 1231 . . 1233 1244 + TstI CACNNNNNNTCC 1224 1219 1256 1251 1234 1237 + SetI ASST 1237 1233 . . 1234 1244 + BsaXI ACNNNNNCTCC 1224 1221 1254 1251 1235 1238 + MnlI CCTC 1245 1244 . . 1244 1249 + NdeI CATATG 1245 1247 . . 1249 1253 + Tsp45I GTSAC 1248 1253 . . 1249 1253 + MaeIII GTNAC 1248 1253 . . 1253 1256 + MaeI CTAG 1253 1255 . . 1255 1258 + SetI ASST 1258 1254 . . 1255 1258 + CviJI RGCY 1256 1256 . . 1255 1258 + AluI AGCT 1256 1256 . . 1256 1262 + SapI GCTCTTC 1263 1266 . . 1257 1262 + Ksp632I* CTCTTC 1263 1266 . . 1258 1262 - MboII GAAGA 1250 1249 . . 1262 1266 + BssKI CCNGG 1261 1266 . . 1262 1266 + BseBI CCWGG 1263 1264 . . 1262 1266 + ScrFI CCNGG 1263 1264 . . 1262 1266 + EcoRII CCWGG 1261 1266 . . 1272 1278 + TspRI CASTGNN 1278 1269 . . 1271 1275 - BsrI ACTGG 1271 1269 . . 1282 1287 + TatI WGTACW 1282 1286 . . 1282 1287 + Bsp1407I TGTACA 1282 1286 . . 1283 1286 + Csp6I GTAC 1283 1285 . . 1283 1286 + RsaI GTAC 1284 1284 . . 1290 1295 + Hpy166II GTNNAC 1292 1292 . . 1290 1295 + SduI GDGCHC 1294 1290 . . 1290 1295 + BseSI GKGCMC 1294 1290 . . 1290 1295 + ApaLI GTGCAC 1290 1294 . . 1290 1295 + HgiAI* GWGCWC 1294 1290 . . 1291 1294 + CviRI* TGCA 1292 1292 . . 1299 1304 + TaqII GACCGA 1315 1313 . . 1299 1307 + Tth111I GACNNNGTC 1302 1303 . . 1297 1301 - BsmAI GTCTC 1291 1295 . . 1297 1302 - Eco31I GGTCTC 1291 1295 . . 1303 1307 + PleI GAGTC 1311 1312 . . 1303 1307 + MlyI GAGTC 1312 1312 . . 1303 1307 + HinfI GANTC 1303 1306 . . 1305 1310 + Hpy166II GTNNAC 1307 1307 . . 1305 1310 + AccI GTMKAC 1306 1308 . . 1297 1307 - Hin4I GAYNNNNNVTC 1320 1315 1288 1283 1310 1314 - BsrI ACTGG 1310 1308 . . 1318 1322 + Tsp45I GTSAC 1317 1322 . . 1318 1322 + MaeIII GTNAC 1317 1322 . . 1325 1330 + NdeI CATATG 1326 1328 . . 1333 1336 + SetI ASST 1336 1332 . . 1333 1336 + CviJI RGCY 1334 1334 . . 1333 1336 + AluI AGCT 1334 1334 . . 1338 1343 + Hpy166II GTNNAC 1340 1340 . . 1338 1343 + AccI GTMKAC 1339 1341 . . 1345 1349 + BlsI GCNGC 1347 1346 . . 1345 1349 + BisI GCNGC 1346 1347 . . 1345 1349 + TseI GCWGC 1345 1348 . . 1348 1352 + TauI GCSGC 1351 1348 . . 1343 1348 - EcoP15I CAGCAG 1315 1317 . . 1348 1352 + BlsI GCNGC 1350 1349 . . 1348 1352 + BisI GCNGC 1349 1350 . . 1345 1349 - BbvI GCAGC 1332 1336 . . 1349 1352 + AciI CCGC 1349 1351 . . 1361 1364 + MaeI CTAG 1361 1363 . . 1363 1366 + SetI ASST 1366 1362 . . 1363 1366 + CviJI RGCY 1364 1364 . . 1363 1366 + AluI AGCT 1364 1364 . . 1380 1386 + TspRI CASTGNN 1386 1377 . . 1379 1383 - BsrI ACTGG 1379 1377 . . 1390 1395 + TatI WGTACW 1390 1394 . . 1390 1395 + Bsp1407I TGTACA 1390 1394 . . 1391 1394 + Csp6I GTAC 1391 1393 . . 1391 1394 + RsaI GTAC 1392 1392 . . 1398 1403 + Hpy166II GTNNAC 1400 1400 . . 1398 1403 + SduI GDGCHC 1402 1398 . . 1398 1403 + BseSI GKGCMC 1402 1398 . . 1398 1403 + ApaLI GTGCAC 1398 1402 . . 1398 1403 + HgiAI* GWGCWC 1402 1398 . . 1399 1402 + CviRI* TGCA 1400 1400 . . 1409 1413 + DdeI CTNAG 1409 1412 . . 1411 1415 + PleI GAGTC 1419 1420 . . 1411 1415 + MlyI GAGTC 1420 1420 . . 1411 1415 + HinfI GANTC 1411 1414 . . 1413 1418 + Hpy166II GTNNAC 1415 1415 . . 1409 1413 - BspCNI CTCAG 1401 1399 . . 1409 1413 - BseMII CTCAG 1400 1398 . . 1413 1418 + AccI GTMKAC 1414 1416 . . 1418 1421 + MaeI CTAG 1418 1420 . . 1422 1433 + TstI CACNNNNNNTCC 1413 1408 1445 1440 1423 1426 + SetI ASST 1426 1422 . . 1423 1433 + BsaXI ACNNNNNCTCC 1413 1410 1443 1440 1424 1427 + MnlI CCTC 1434 1433 . . 1433 1438 + NdeI CATATG 1434 1436 . . 1438 1442 + Tsp45I GTSAC 1437 1442 . . 1438 1442 + MaeIII GTNAC 1437 1442 . . 1442 1445 + MaeI CTAG 1442 1444 . . 1444 1447 + SetI ASST 1447 1443 . . 1444 1447 + CviJI RGCY 1445 1445 . . 1444 1447 + AluI AGCT 1445 1445 . . 1445 1451 + SapI GCTCTTC 1452 1455 . . 1446 1451 + Ksp632I* CTCTTC 1452 1455 . . 1451 1454 + MnlI CCTC 1461 1460 . . 1447 1451 - MboII GAAGA 1439 1438 . . 1454 1458 + Hpy99I CGWCG 1458 1453 . . 1461 1467 + TspRI CASTGNN 1467 1458 . . 1460 1464 - BsrI ACTGG 1460 1458 . . 1471 1476 + TatI WGTACW 1471 1475 . . 1471 1476 + Bsp1407I TGTACA 1471 1475 . . 1472 1475 + Csp6I GTAC 1472 1474 . . 1472 1475 + RsaI GTAC 1473 1473 . . 1479 1484 + Hpy166II GTNNAC 1481 1481 . . 1479 1484 + SduI GDGCHC 1483 1479 . . 1479 1484 + BseSI GKGCMC 1483 1479 . . 1479 1484 + ApaLI GTGCAC 1479 1483 . . 1479 1484 + HgiAI* GWGCWC 1483 1479 . . 1480 1483 + CviRI* TGCA 1481 1481 . . 1488 1493 + TaqII GACCGA 1504 1502 . . 1488 1496 + Tth111I GACNNNGTC 1491 1492 . . 1486 1490 - BsmAI GTCTC 1480 1484 . . 1486 1491 - Eco31I GGTCTC 1480 1484 . . 1492 1496 + PleI GAGTC 1500 1501 . . 1492 1496 + MlyI GAGTC 1501 1501 . . 1492 1496 + HinfI GANTC 1492 1495 . . 1494 1499 + Hpy166II GTNNAC 1496 1496 . . 1494 1499 + AccI GTMKAC 1495 1497 . . 1486 1496 - Hin4I GAYNNNNNVTC 1509 1504 1477 1472 1499 1503 - BsrI ACTGG 1499 1497 . . 1507 1511 + Tsp45I GTSAC 1506 1511 . . 1507 1511 + MaeIII GTNAC 1506 1511 . . 1514 1519 + NdeI CATATG 1515 1517 . . 1522 1525 + SetI ASST 1525 1521 . . 1522 1525 + CviJI RGCY 1523 1523 . . 1522 1525 + AluI AGCT 1523 1523 . . 1527 1532 + Hpy166II GTNNAC 1529 1529 . . 1527 1532 + AccI GTMKAC 1528 1530 . . 1534 1538 + BlsI GCNGC 1536 1535 . . 1534 1538 + BisI GCNGC 1535 1536 . . 1534 1538 + TseI GCWGC 1534 1537 . . 1537 1541 + TauI GCSGC 1540 1537 . . 1532 1537 - EcoP15I CAGCAG 1504 1506 . . 1537 1541 + BlsI GCNGC 1539 1538 . . 1537 1541 + BisI GCNGC 1538 1539 . . 1534 1538 - BbvI GCAGC 1521 1525 . . 1538 1541 + AciI CCGC 1538 1540 . . 1550 1553 + MaeI CTAG 1550 1552 . . 1552 1555 + SetI ASST 1555 1551 . . 1552 1555 + CviJI RGCY 1553 1553 . . 1552 1555 + AluI AGCT 1553 1553 . . 1569 1575 + TspRI CASTGNN 1575 1566 . . 1568 1572 - BsrI ACTGG 1568 1566 . . 1579 1584 + TatI WGTACW 1579 1583 . . 1579 1584 + Bsp1407I TGTACA 1579 1583 . . 1580 1583 + Csp6I GTAC 1580 1582 . . 1580 1583 + RsaI GTAC 1581 1581 . . 1587 1592 + Hpy166II GTNNAC 1589 1589 . . 1587 1592 + SduI GDGCHC 1591 1587 . . 1587 1592 + BseSI GKGCMC 1591 1587 . . 1587 1592 + ApaLI GTGCAC 1587 1591 . . 1587 1592 + HgiAI* GWGCWC 1591 1587 . . 1588 1591 + CviRI* TGCA 1589 1589 . . 1600 1604 + PleI GAGTC 1608 1609 . . 1600 1604 + MlyI GAGTC 1609 1609 . . 1600 1604 + HinfI GANTC 1600 1603 . . 1602 1607 + Hpy166II GTNNAC 1604 1604 . . 1602 1607 + AccI GTMKAC 1603 1605 . . 1607 1610 + MaeI CTAG 1607 1609 . . 1611 1622 + TstI CACNNNNNNTCC 1602 1597 1634 1629 1612 1615 + SetI ASST 1615 1611 . . 1612 1622 + BsaXI ACNNNNNCTCC 1602 1599 1632 1629 1613 1616 + MnlI CCTC 1623 1622 . . 1622 1627 + NdeI CATATG 1623 1625 . . 1627 1631 + Tsp45I GTSAC 1626 1631 . . 1627 1631 + MaeIII GTNAC 1626 1631 . . 1628 1632 - HphI GGTGA 1620 1619 . . 1633 1636 + SetI ASST 1636 1632 . . 1633 1636 + CviJI RGCY 1634 1634 . . 1633 1636 + AluI AGCT 1634 1634 . . 1634 1640 + SapI GCTCTTC 1641 1644 . . 1635 1640 + Ksp632I* CTCTTC 1641 1644 . . 1636 1640 - MboII GAAGA 1628 1627 . . 1640 1644 + BssKI CCNGG 1639 1644 . . 1640 1644 + BseBI CCWGG 1641 1642 . . 1640 1644 + ScrFI CCNGG 1641 1642 . . 1640 1644 + EcoRII CCWGG 1639 1644 . . 1650 1656 + TspRI CASTGNN 1656 1647 . . 1649 1653 - BsrI ACTGG 1649 1647 . . 1660 1665 + TatI WGTACW 1660 1664 . . 1660 1665 + Bsp1407I TGTACA 1660 1664 . . 1661 1664 + Csp6I GTAC 1661 1663 . . 1661 1664 + RsaI GTAC 1662 1662 . . 1668 1673 + Hpy166II GTNNAC 1670 1670 . . 1668 1673 + SduI GDGCHC 1672 1668 . . 1668 1673 + BseSI GKGCMC 1672 1668 . . 1668 1673 + ApaLI GTGCAC 1668 1672 . . 1668 1673 + HgiAI* GWGCWC 1672 1668 . . 1669 1672 + CviRI* TGCA 1670 1670 . . 1677 1682 + TaqII GACCGA 1693 1691 . . 1677 1685 + Tth111I GACNNNGTC 1680 1681 . . 1675 1679 - BsmAI GTCTC 1669 1673 . . 1675 1680 - Eco31I GGTCTC 1669 1673 . . 1681 1685 + PleI GAGTC 1689 1690 . . 1681 1685 + MlyI GAGTC 1690 1690 . . 1681 1685 + HinfI GANTC 1681 1684 . . 1683 1688 + Hpy166II GTNNAC 1685 1685 . . 1683 1688 + AccI GTMKAC 1684 1686 . . 1675 1685 - Hin4I GAYNNNNNVTC 1698 1693 1666 1661 1688 1692 - BsrI ACTGG 1688 1686 . . 1696 1700 + Tsp45I GTSAC 1695 1700 . . 1696 1700 + MaeIII GTNAC 1695 1700 . . 1703 1708 + NdeI CATATG 1704 1706 . . 1711 1714 + SetI ASST 1714 1710 . . 1711 1714 + CviJI RGCY 1712 1712 . . 1711 1714 + AluI AGCT 1712 1712 . . 1716 1721 + Hpy166II GTNNAC 1718 1718 . . 1716 1721 + AccI GTMKAC 1717 1719 . . 1723 1727 + BlsI GCNGC 1725 1724 . . 1723 1727 + BisI GCNGC 1724 1725 . . 1723 1727 + TseI GCWGC 1723 1726 . . 1726 1730 + TauI GCSGC 1729 1726 . . 1721 1726 - EcoP15I CAGCAG 1693 1695 . . 1726 1730 + BlsI GCNGC 1728 1727 . . 1726 1730 + BisI GCNGC 1727 1728 . . 1723 1727 - BbvI GCAGC 1710 1714 . . 1727 1730 + AciI CCGC 1727 1729 . . 1739 1742 + MaeI CTAG 1739 1741 . . 1741 1744 + SetI ASST 1744 1740 . . 1741 1744 + CviJI RGCY 1742 1742 . . 1741 1744 + AluI AGCT 1742 1742 . . 1758 1764 + TspRI CASTGNN 1764 1755 . . 1757 1761 - BsrI ACTGG 1757 1755 . . 1768 1773 + TatI WGTACW 1768 1772 . . 1768 1773 + Bsp1407I TGTACA 1768 1772 . . 1769 1772 + Csp6I GTAC 1769 1771 . . 1769 1772 + RsaI GTAC 1770 1770 . . 1776 1781 + Hpy166II GTNNAC 1778 1778 . . 1776 1781 + SduI GDGCHC 1780 1776 . . 1776 1781 + BseSI GKGCMC 1780 1776 . . 1776 1781 + ApaLI GTGCAC 1776 1780 . . 1776 1781 + HgiAI* GWGCWC 1780 1776 . . 1777 1780 + CviRI* TGCA 1778 1778 . . 1789 1793 + PleI GAGTC 1797 1798 . . 1789 1793 + MlyI GAGTC 1798 1798 . . 1789 1793 + HinfI GANTC 1789 1792 . . 1791 1796 + Hpy166II GTNNAC 1793 1793 . . 1791 1796 + AccI GTMKAC 1792 1794 . . 1800 1811 + TstI CACNNNNNNTCC 1791 1786 1823 1818 1801 1811 + BsaXI ACNNNNNCTCC 1791 1788 1821 1818 1802 1806 - FokI GGATG 1788 1792 . . 1802 1806 - BseGI GGATG 1801 1799 . . 1811 1816 + NdeI CATATG 1812 1814 . . 1815 1818 + CviRI* TGCA 1816 1816 . . 1819 1822 + SetI ASST 1822 1818 . . 1820 1823 + MnlI CCTC 1830 1829 . . 1828 1832 + BsrI ACTGG 1833 1831 . . 1836 1839 + SetI ASST 1839 1835 . . 1836 1839 + CviJI RGCY 1837 1837 . . 1836 1839 + AluI AGCT 1837 1837 . . 1842 1846 - TspDTI ATGAA 1832 1830 . . 1850 1853 + AciI CCGC 1850 1852 . . 1868 1882 + XcmI CCANNNNNNNNNTGG 1875 1874 . . 1869 1872 + FatI CATG 1868 1872 . . 1869 1872 + NlaIII CATG 1872 1868 . . 1869 1872 + CviAII CATG 1869 1871 . . 1872 1877 + ZraI GACGTC 1874 1874 . . 1872 1877 + AcyI GRCGYC 1873 1875 . . 1872 1877 + AatII GACGTC 1876 1872 . . 1873 1876 + TaiI ACGT 1876 1872 . . 1873 1876 + SetI ASST 1876 1872 . . 1873 1876 + MaeII ACGT 1873 1875 . . 1881 1885 + BmgT120I GGNCC 1882 1883 . . 1881 1885 + AvaII GGWCC 1881 1884 . . 1881 1885 + AsuI* GGNCC 1881 1884 . . 1897 1901 + Tsp4CI* ACNGT 1899 1898 . . 1909 1912 + CviJI RGCY 1910 1910 . . 1915 1919 - MboII GAAGA 1907 1906 . . 1921 1925 + Tsp4CI* ACNGT 1923 1922 . . 1928 1933 + Cac8I GCNNGC 1930 1930 . . 1936 1946 + BsaXI ACNNNNNCTCC 1926 1923 1956 1953 1944 1949 + MmeI TCCRAC 1969 1967 . . 1950 1954 - TspDTI ATGAA 1940 1938 . . 1955 1960 + SfeI* CTRYAG 1955 1959 . . 1960 1964 + PleI GAGTC 1968 1969 . . 1960 1964 + MlyI GAGTC 1969 1969 . . 1960 1964 + HinfI GANTC 1960 1963 . . 1970 1973 + MaeI CTAG 1970 1972 . . 1972 1977 + TatI WGTACW 1972 1976 . . 1972 1977 + ScaI AGTACT 1974 1974 . . 1973 1976 + Csp6I GTAC 1973 1975 . . 1973 1976 + RsaI GTAC 1974 1974 . . 1978 1982 - TspDTI ATGAA 1968 1966 . . 1997 2001 + BlsI GCNGC 1999 1998 . . 1997 2001 + BisI GCNGC 1998 1999 . . 1997 2001 + BbvI GCAGC 2009 2013 . . 1997 2001 + TseI GCWGC 1997 2000 . . 2003 2006 + MseI TTAA 2003 2005 . . 2014 2018 + MaeIII GTNAC 2013 2018 . . 2017 2020 + TaiI ACGT 2020 2016 . . 2017 2020 + SetI ASST 2020 2016 . . 2017 2020 + MaeII ACGT 2017 2019 . . 2021 2025 - FokI GGATG 2007 2011 . . 2021 2025 - BseGI GGATG 2020 2018 . . 2043 2046 + FatI CATG 2042 2046 . . 2043 2046 + NlaIII CATG 2046 2042 . . 2043 2046 + CviAII CATG 2043 2045 . . 2046 2051 + BciVI GTATCC 2057 2056 . . 2051 2054 + MaeI CTAG 2051 2053 . . 2054 2058 + MaeIII GTNAC 2053 2058 . . 2055 2060 + TsoI TARCCA 2071 2069 . . 2062 2066 + Tsp4CI* ACNGT 2064 2063 . . 2065 2069 + MaeIII GTNAC 2064 2069 . . 2072 2075 + MaeI CTAG 2072 2074 . . 2074 2077 + SetI ASST 2077 2073 . . 2074 2077 + CviJI RGCY 2075 2075 . . 2074 2077 + AluI AGCT 2075 2075 . . 2083 2087 + BsmAI GTCTC 2088 2092 . . 2094 2097 + TspEI AATT 2093 2097 . . 2101 2105 + Hpy188I TCNGA 2103 2102 . . 2104 2108 + TfiI GAWTC 2104 2107 . . 2104 2108 + HinfI GANTC 2104 2107 . . 2110 2114 + Hpy188I TCNGA 2112 2111 . . 2107 2111 - MboII GAAGA 2099 2098 . . 2113 2117 + TfiI GAWTC 2113 2116 . . 2113 2117 + HinfI GANTC 2113 2116 . . 2117 2120 + MnlI CCTC 2127 2126 . . 2117 2122 + SecI* CCNNGG 2117 2121 . . 2121 2124 + CviJI RGCY 2122 2122 . . 2121 2124 + HaeIII GGCC 2122 2122 . . 2118 2123 - NmeAIII GCCGAG 2098 2096 . . 2123 2126 + MnlI CCTC 2133 2132 . . 2124 2128 + DdeI CTNAG 2124 2127 . . 2124 2128 + BspCNI CTCAG 2137 2135 . . 2124 2128 + BseMII CTCAG 2138 2136 . . 2128 2132 + MaeIII GTNAC 2127 2132 . . 2141 2144 + MnlI CCTC 2151 2150 . . 2155 2159 + Hpy188I TCNGA 2157 2156 . . 2158 2163 + EcoRI GAATTC 2158 2162 . . 2158 2163 + ApoI RAATTY 2158 2162 . . 2159 2162 + TspEI AATT 2158 2162 . . 2167 2171 + BccI CCATC 2175 2176 . . 2176 2180 + TspDTI ATGAA 2191 2189 . . 2186 2189 + SetI ASST 2189 2185 . . 2184 2188 - Hin4II* CCTTC 2178 2177 . . 2201 2204 + MnlI CCTC 2211 2210 . . 2199 2204 - BseRI GAGGAG 2190 2188 . . 2206 2210 - HphI GGTGA 2198 2197 . . 2215 2218 + TspEI AATT 2214 2218 . . 2227 2230 + SetI ASST 2230 2226 . . 2227 2230 + CviJI RGCY 2228 2228 . . 2227 2230 + AluI AGCT 2228 2228 . . 2233 2243 + Hin4I GAYNNNNNVTC 2224 2219 2256 2251 2233 2243 - Hin4I GAYNNNNNVTC 2256 2251 2224 2219 2243 2247 + DdeI CTNAG 2243 2246 . . 2248 2252 + TfiI GAWTC 2248 2251 . . 2248 2252 + HinfI GANTC 2248 2251 . . 2254 2258 + Tsp4CI* ACNGT 2256 2255 . . 2262 2266 - TspDTI ATGAA 2252 2250 . . 2264 2268 - FokI GGATG 2250 2254 . . 2264 2268 - BseGI GGATG 2263 2261 . . 2281 2284 + CviJI RGCY 2282 2282 . . 2286 2289 + DpnI GATC 2287 2287 . . 2286 2289 + BstKTI GATC 2288 2286 . . 2286 2289 + MboI GATC 2285 2289 . . 2291 2294 + MaeI CTAG 2291 2293 . . 2305 2318 - AjuI GAANNNNNNNTTGG 2330 2325 2298 2293 2319 2322 + SetI ASST 2322 2318 . . 2319 2322 + CviJI RGCY 2320 2320 . . 2319 2322 + AluI AGCT 2320 2320 . . 2324 2327 + MaeI CTAG 2324 2326 . . 2334 2339 + SspI AATATT 2336 2336 . . 2344 2347 + SetI ASST 2347 2343 . . 2344 2347 + CviJI RGCY 2345 2345 . . 2344 2347 + AluI AGCT 2345 2345 . . 2348 2352 + BccI CCATC 2356 2357 . . 2363 2367 + Tsp4CI* ACNGT 2365 2364 . . 2374 2377 + MseI TTAA 2374 2376 . . 2379 2389 + FalI AAGNNNNNCTT 2370 2365 2402 2397 2389 2392 + TaqI TCGA 2389 2391 . . 2379 2389 - FalI AAGNNNNNCTT 2402 2397 2370 2365 2386 2390 - MboII GAAGA 2378 2377 . . 2391 2400 + PshAI GACNNNNGTC 2395 2395 . . 2405 2408 + MnlI CCTC 2415 2414 . . 2406 2410 + DdeI CTNAG 2406 2409 . . 2406 2410 + BspCNI CTCAG 2419 2417 . . 2406 2410 + BseMII CTCAG 2420 2418 . . 2407 2411 + Hpy188I TCNGA 2409 2408 . . 2410 2414 + TfiI GAWTC 2410 2413 . . 2410 2414 + HinfI GANTC 2410 2413 . . 2416 2420 + Hpy188I TCNGA 2418 2417 . . 2413 2417 - MboII GAAGA 2405 2404 . . 2419 2423 + TfiI GAWTC 2419 2422 . . 2419 2423 + HinfI GANTC 2419 2422 . . 2432 2437 - BtgZI GCGATG 2417 2421 . . 2448 2454 + TspRI CASTGNN 2454 2445 . . 2444 2448 - FokI GGATG 2430 2434 . . 2444 2448 - BseGI GGATG 2443 2441 . . 2447 2451 - BsrI ACTGG 2447 2445 . . 2452 2456 + TfiI GAWTC 2452 2455 . . 2452 2456 + HinfI GANTC 2452 2455 . . 2459 2463 + SfaNI GCATC 2468 2472 . . 2467 2471 - MboII GAAGA 2459 2458 . . 2468 2473 - Eco57MI CTGRAG 2453 2451 . . 2468 2473 - Eco57I CTGAAG 2453 2451 . . 2483 2486 + TaqI TCGA 2483 2485 . . 2485 2489 + TfiI GAWTC 2485 2488 . . 2485 2489 + HinfI GANTC 2485 2488 . . 2493 2503 + FalI AAGNNNNNCTT 2484 2479 2516 2511 2495 2498 + Csp6I GTAC 2495 2497 . . 2495 2498 + RsaI GTAC 2496 2496 . . 2497 2500 + SetI ASST 2500 2496 . . 2498 2501 + MnlI CCTC 2508 2507 . . 2499 2504 + Ksp632I* CTCTTC 2505 2508 . . 2493 2503 - FalI AAGNNNNNCTT 2516 2511 2484 2479 2500 2504 - MboII GAAGA 2492 2491 . . 2509 2512 + SetI ASST 2512 2508 . . 2509 2512 + CviJI RGCY 2510 2510 . . 2509 2512 + AluI AGCT 2510 2510 . . 2514 2519 + Hpy178III* TCNNGA 2515 2517 . . 2525 2530 + SfeI* CTRYAG 2525 2529 . . 2529 2532 + CviJI RGCY 2530 2530 . . 2531 2534 + MnlI CCTC 2541 2540 . . 2536 2539 + SetI ASST 2539 2535 . . 2537 2540 + MnlI CCTC 2547 2546 . . 2539 2543 - TspGWI ACGGA 2529 2527 . . 2551 2555 - MboII GAAGA 2543 2542 . . 2555 2559 + DdeI CTNAG 2555 2558 . . 2555 2559 + BspCNI CTCAG 2568 2566 . . 2555 2559 + BseMII CTCAG 2569 2567 . . 2563 2567 + Tsp45I GTSAC 2562 2567 . . 2563 2567 + MaeIII GTNAC 2562 2567 . . 2565 2569 + HinfI GANTC 2565 2568 . . 2565 2569 - PleI GAGTC 2559 2560 . . 2565 2569 - MlyI GAGTC 2559 2559 . . 2569 2573 + BccI CCATC 2577 2578 . . 2573 2576 + MnlI CCTC 2583 2582 . . 2570 2574 - FokI GGATG 2556 2560 . . 2570 2574 - BseGI GGATG 2569 2567 . . 2587 2591 + Tsp4CI* ACNGT 2589 2588 . . 2596 2599 + MnlI CCTC 2606 2605 . . 2603 2608 + SfeI* CTRYAG 2603 2607 . . 2608 2613 + SduI GDGCHC 2612 2608 . . 2608 2613 + HgiJII* GRGCYC 2612 2608 . . 2609 2612 + CviJI RGCY 2610 2610 . . 2629 2634 + TatI WGTACW 2629 2633 . . 2630 2633 + Csp6I GTAC 2630 2632 . . 2630 2633 + RsaI GTAC 2631 2631 . . 2634 2639 + ApoI RAATTY 2634 2638 . . 2635 2638 + TspEI AATT 2634 2638 . . 2638 2642 - MboII GAAGA 2630 2629 . . 2642 2647 + StyI CCWWGG 2642 2646 . . 2642 2647 + SecI* CCNNGG 2642 2646 . . 2630 2642 - BarI GAAGNNNNNNTAC 2654 2649 2622 2617 2652 2655 + FatI CATG 2651 2655 . . 2652 2655 + NlaIII CATG 2655 2651 . . 2652 2655 + CviAII CATG 2652 2654 . . 2659 2663 + Hpy188I TCNGA 2661 2660 . . 2668 2673 + HaeII RGCGCY 2672 2668 . . 2669 2672 + Hin6I GCGC 2669 2671 . . 2669 2672 + GlaI GCGC 2670 2670 . . 2669 2672 + HhaI GCGC 2671 2669 . . 2671 2676 + Cac8I GCNNGC 2673 2673 . . 2683 2687 + Hin4II* CCTTC 2693 2692 . . 2686 2690 - MboII GAAGA 2678 2677 . . 2688 2692 - TspDTI ATGAA 2678 2676 . . 2694 2697 + SetI ASST 2697 2693 . . 2694 2697 + CviJI RGCY 2695 2695 . . 2694 2697 + AluI AGCT 2695 2695 . . 2704 2714 + BsiYI* CCNNNNNNNGG 2710 2707 . . 2719 2723 + MaeIII GTNAC 2718 2723 . . 2724 2730 + TspRI CASTGNN 2730 2721 . . 2723 2727 - BsrI ACTGG 2723 2721 . . 2729 2733 + TspDTI ATGAA 2744 2742 . . 2740 2745 + Hpy178III* TCNNGA 2741 2743 . . 2740 2745 + BetI* WCCGGW 2740 2744 . . 2740 2745 + BspMII* TCCGGA 2740 2744 . . 2737 2741 - MboII GAAGA 2729 2728 . . 2741 2744 + HpaII CCGG 2741 2743 . . 2751 2756 + Hpy178III* TCNNGA 2752 2754 . . 2755 2758 + DpnI GATC 2756 2756 . . 2755 2758 + BstKTI GATC 2757 2755 . . 2755 2758 + MboI GATC 2754 2758 . . 2750 2755 - BsiI* CACGAG 2750 2754 . . 2760 2769 + MslI CAYNNNNRTG 2764 2764 . . 2760 2769 + OliI CACNNNNGTG 2764 2764 . . 2763 2769 + TspRI CASTGNN 2769 2760 . . 2764 2768 + Tsp4CI* ACNGT 2766 2765 . . 2774 2778 + FokI GGATG 2787 2791 . . 2774 2778 + BseGI GGATG 2780 2778 . . 2782 2786 + Hpy188I TCNGA 2784 2783 . . 2780 2785 - Eco57MI CTGRAG 2765 2763 . . 2780 2785 - Eco57I CTGAAG 2765 2763 . . 2792 2795 + MnlI CCTC 2802 2801 . . 2801 2805 + TspDTI ATGAA 2816 2814 . . 2821 2825 + Tsp4CI* ACNGT 2823 2822 . . 2836 2840 + TfiI GAWTC 2836 2839 . . 2836 2840 + HinfI GANTC 2836 2839 . . 2845 2848 + SetI ASST 2848 2844 . . 2845 2848 + CviJI RGCY 2846 2846 . . 2845 2848 + AluI AGCT 2846 2846 . . 2846 2850 + BlsI GCNGC 2848 2847 . . 2846 2850 + BisI GCNGC 2847 2848 . . 2846 2850 + TseI GCWGC 2846 2849 . . 2846 2850 - BbvI GCAGC 2833 2837 . . 2857 2861 + Tsp4CI* ACNGT 2859 2858 . . 2860 2864 + BsmAI GTCTC 2865 2869 . . 2862 2867 + SmlI CTYRAG 2862 2866 . . 2862 2867 - Bce83I* CTTGAG 2847 2845 . . 2870 2875 + SfeI* CTRYAG 2870 2874 . . 2879 2884 + DsaI* CCRYGG 2879 2883 . . 2879 2884 + SecI* CCNNGG 2879 2883 . . 2881 2885 + BceAI ACGGC 2897 2899 . . 2882 2887 + CfrI YGGCCR 2882 2886 . . 2883 2886 + CviJI RGCY 2884 2884 . . 2883 2886 + HaeIII GGCC 2884 2884 . . 2894 2898 + BccI CCATC 2902 2903 . . 2894 2904 + BsiYI* CCNNNNNNNGG 2900 2897 . . 2910 2916 + TspRI CASTGNN 2916 2907 . . 2924 2927 + FatI CATG 2923 2927 . . 2924 2927 + NlaIII CATG 2927 2923 . . 2924 2927 + CviAII CATG 2924 2926 . . 2927 2932 + NlaIV GGNNCC 2929 2929 . . 2927 2932 + HgiCI* GGYRCC 2927 2931 . . 2928 2933 + SduI GDGCHC 2932 2928 . . 2928 2933 + BseSI GKGCMC 2932 2928 . . 2932 2935 + MnlI CCTC 2942 2941 . . 2944 2948 + TspGWI ACGGA 2959 2957 . . 2948 2951 + TspEI AATT 2947 2951 . . 2950 2953 + MseI TTAA 2950 2952 . . 2956 2960 + Tsp4CI* ACNGT 2958 2957 . . 2966 2969 + TspEI AATT 2965 2969 . . 2971 2975 + PleI GAGTC 2979 2980 . . 2971 2975 + MlyI GAGTC 2980 2980 . . 2971 2975 + HinfI GANTC 2971 2974 . . 2974 2978 + Hpy188I TCNGA 2976 2975 . . 2977 2981 + MboII GAAGA 2989 2988 . . 2991 3001 + BsiYI* CCNNNNNNNGG 2997 2994 . . 2994 2997 + TspEI AATT 2993 2997 . . 2998 3002 + Tsp4CI* ACNGT 3000 2999 . . 3001 3005 + MaeIII GTNAC 3000 3005 . . 3013 3017 + PleI GAGTC 3021 3022 . . 3013 3017 + MlyI GAGTC 3022 3022 . . 3013 3017 + HinfI GANTC 3013 3016 . . 3028 3032 + Hpy188I TCNGA 3030 3029 . . 3037 3047 + MwoI GCNNNNNNNGC 3043 3040 . . 3041 3047 + AarI CACCTGC 3051 3055 . . 3042 3045 + SetI ASST 3045 3041 . . 3042 3047 + BspMI ACCTGC 3051 3055 . . 3040 3044 - HphI GGTGA 3032 3031 . . 3055 3058 + TaqI TCGA 3055 3057 . . 3059 3067 + AlwNI CAGNNNCTG 3064 3061 . . 3060 3063 + CviJI RGCY 3061 3061 . . 3063 3069 + TspRI CASTGNN 3069 3060 . . 3063 3068 - BtsI GCAGTG 3062 3060 . . 3070 3074 + Tsp4CI* ACNGT 3072 3071 . . 3085 3089 + MaeIII GTNAC 3084 3089 . . 3088 3092 + Tsp4CI* ACNGT 3090 3089 . . 3101 3104 + FatI CATG 3100 3104 . . 3101 3104 + NlaIII CATG 3104 3100 . . 3101 3104 + CviAII CATG 3101 3103 . . 3104 3109 + NlaIV GGNNCC 3106 3106 . . 3105 3110 + SduI GDGCHC 3109 3105 . . 3105 3110 + HgiJII* GRGCYC 3109 3105 . . 3106 3109 + CviJI RGCY 3107 3107 . . 3114 3117 + CviJI RGCY 3115 3115 . . 3127 3130 + MaeI CTAG 3127 3129 . . 3130 3133 - AciI CCGC 3130 3132 . . 3139 3143 + Hpy188I TCNGA 3141 3140 . . 3146 3149 + TaqI TCGA 3146 3148 . . 3147 3158 + BcgI CGANNNNNNTGC 3136 3134 3170 3168 3157 3167 + MwoI GCNNNNNNNGC 3163 3160 . . 3157 3167 + BglI GCCNNNNNGGC 3163 3160 . . 3158 3162 - BsrI ACTGG 3158 3156 . . 3165 3168 + CviJI RGCY 3166 3166 . . 3168 3172 - TspDTI ATGAA 3158 3156 . . 3178 3182 + Hpy188I TCNGA 3180 3179 . . 3179 3187 + AlwNI CAGNNNCTG 3184 3181 . . 3183 3186 + CviJI RGCY 3184 3184 . . 3181 3184 - MnlI CCTC 3174 3173 . . 3184 3188 + BlsI GCNGC 3186 3185 . . 3184 3188 + BisI GCNGC 3185 3186 . . 3184 3188 + TseI GCWGC 3184 3187 . . 3188 3192 + DdeI CTNAG 3188 3191 . . 3184 3188 - BbvI GCAGC 3171 3175 . . 3185 3190 - EcoP15I CAGCAG 3157 3159 . . 3188 3192 - BspCNI CTCAG 3180 3178 . . 3188 3192 - BseMII CTCAG 3179 3177 . . 3190 3194 - BsmAI GTCTC 3184 3188 . . 3190 3200 - Hin4I GAYNNNNNVTC 3213 3208 3181 3176 3217 3220 + TspEI AATT 3216 3220 . . 3225 3230 + MmeI TCCRAC 3250 3248 . . 3226 3236 + BsiYI* CCNNNNNNNGG 3232 3229 . . 3226 3236 + PflMI CCANNNNNTGG 3232 3229 . . 3235 3239 + BslFI GGGAC 3249 3253 . . 3275 3280 + MluI ACGCGT 3275 3279 . . 3275 3280 + AflIII ACRYGT 3275 3279 . . 3276 3279 + FnuDII* CGCG 3277 3277 . . 3280 3284 + Hpy188I TCNGA 3282 3281 . . 3277 3281 - HgaI GACGC 3266 3271 . . 3304 3314 + MwoI GCNNNNNNNGC 3310 3307 . . 3307 3311 + Hpy188I TCNGA 3309 3308 . . 3308 3312 + DdeI CTNAG 3308 3311 . . 3312 3315 + CviJI RGCY 3313 3313 . . 3308 3312 - BspCNI CTCAG 3300 3298 . . 3308 3312 - BseMII CTCAG 3299 3297 . . 3310 3313 - MnlI CCTC 3303 3302 . . 3331 3335 + Tsp45I GTSAC 3330 3335 . . 3331 3335 + MaeIII GTNAC 3330 3335 . . 3365 3368 + CviJI RGCY 3366 3366 . . 3367 3370 + MnlI CCTC 3377 3376 . . 3368 3373 - BsiI* CACGAG 3368 3372 . . 3397 3402 + XhoII RGATCY 3397 3401 . . 3397 3401 + BinI* GGATC 3405 3406 . . 3398 3401 + DpnI GATC 3399 3399 . . 3398 3401 + BstKTI GATC 3400 3398 . . 3398 3401 + MboI GATC 3397 3401 . . 3401 3404 + MaeI CTAG 3401 3403 . . 3403 3408 + TatI WGTACW 3403 3407 . . 3404 3407 + Csp6I GTAC 3404 3406 . . 3404 3407 + RsaI GTAC 3405 3405 . . 3408 3411 + CviJI RGCY 3409 3409 . . 3410 3413 + MnlI CCTC 3420 3419 . . 3427 3430 + SetI ASST 3430 3426 . . 3427 3430 + CviJI RGCY 3428 3428 . . 3427 3430 + AluI AGCT 3428 3428 . . 3428 3438 + MwoI GCNNNNNNNGC 3434 3431 . . 3431 3434 + SetI ASST 3434 3430 . . 3432 3435 + MnlI CCTC 3442 3441 . . 3432 3437 + SecI* CCNNGG 3432 3436 . . 3433 3438 - NmeAIII GCCGAG 3413 3411 . . 3441 3444 + CviRI* TGCA 3442 3442 . . 3438 3443 - BsrDI GCAATG 3437 3435 . . 3455 3460 + TsoI TARCCA 3471 3469 . . 3478 3482 - TspDTI ATGAA 3468 3466 . . 3485 3488 + MnlI CCTC 3495 3494 . . 3480 3485 - BsrDI GCAATG 3479 3477 . . 3489 3499 + BsiYI* CCNNNNNNNGG 3495 3492 . . 3495 3499 + BsrI ACTGG 3500 3498 . . 3507 3510 + MseI TTAA 3507 3509 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 459 3051 # # AatII: # [GACGTC] # 1634 1876 # # Acc65I: # [GGTACC] # 514 2996 # # AccI: # [GTMKAC] # 33 33 33 33 75 75 # 75 75 81 81 81 81 # 81 955 1718 # # AciI: # [CCGC] # 123 189 189 189 380 1160 # 1280 # # AcyI: # [GRCGYC] # 1637 1873 # # AflIII: # [ACRYGT] # 235 692 2583 # # AjuI: # [GAANNNNNNNTTGG] # 32 1180 2298 # # AluI: # [AGCT] # 25 30 30 30 30 39 # 55 78 78 78 78 81 # 81 81 81 82 92 95 # 139 140 151 153 165 185 # 238 582 613 # # AlwNI: # [CAGNNNCTG] # 120 326 3064 # # ApaLI: # [GTGCAC] # 81 81 81 81 108 108 # 108 108 1020 1734 # # ApoI: # [RAATTY] # 476 876 2158 # # AsuI*: # [GGNCC] # 1629 1881 # # AvaII: # [GGWCC] # 1629 1881 # # BaeI: # [ACNNNNGTAYC] # 33 33 66 776 2602 # # BarI: # [GAAGNNNNNNTAC] # 32 856 2622 # # BbvI: # [GCAGC] # 189 189 189 299 338 339 # 408 735 824 # # BccI: # [CCATC] # 12 181 221 247 325 516 # 608 1400 # # Bce83I*: # [CTTGAG] # 663 827 2020 # # BceAI: # [ACGGC] # 613 2897 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 228 280 340 # 2560 # # BciVI: # [GTATCC] # 753 1304 1453 # # BetI*: # [WCCGGW] # 770 2740 # # BfiI: # [ACTGGG] # 217 3293 # # BglI: # [GCCNNNNNGGC] # 347 3163 # # BglII: # [AGATCT] # 438 3072 # # BinI*: # [GGATC] # 105 3405 # # BisI: # [GCNGC] # 3 3 3 3 11 186 # 186 186 271 325 338 411 # 735 849 # # BlsI: # [GCNGC] # 3 3 3 3 12 186 # 186 186 271 324 338 411 # 735 849 # # BmgT120I: # [GGNCC] # 1628 1882 # # BsaBI: # [GATNNNNATC] # 410 3100 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 30 30 30 # 30 105 159 159 159 159 # 445 560 1554 # # BseBI: # [CCWGG] # 81 165 270 322 378 425 # 1869 # # BseGI: # [GGATG] # 126 180 211 219 243 251 # 298 730 1252 # # BseMII: # [CTCAG] # 84 108 120 149 189 211 # 240 282 377 402 610 738 # # BseRI: # [GAGGAG] # 1320 2190 # # BseSI: # [GKGCMC] # 81 81 81 81 108 108 # 108 108 578 1024 1152 # # BsiI*: # [CACGAG] # 142 618 2750 # # BsiYI*: # [CCNNNNNNNGG] # 15 97 190 235 249 262 # 263 2199 # # BslFI: # [GGGAC] # 261 3249 # # BsmAI: # [GTCTC] # 81 189 189 270 319 326 # 419 777 940 # # Bsp1407I: # [TGTACA] # 81 81 81 81 108 108 # 108 189 931 1742 # # BspCNI: # [CTCAG] # 84 108 120 149 189 210 # 240 282 378 402 612 736 # # BspLU11I*: # [ACATGT] # 692 2818 # # BspMI: # [ACCTGC] # 459 3051 # # BspMII*: # [TCCGGA] # 770 2740 # # BsrDI: # [GCAATG] # 31 42 3437 # # BsrI: # [ACTGG] # 10 39 39 39 39 39 # 42 69 69 69 69 76 # 81 81 81 81 83 102 # 119 212 276 342 404 435 # 614 # # BssKI: # [CCNGG] # 81 165 270 320 378 425 # 1871 # # BstKTI: # [GATC] # 27 110 278 414 469 643 # 1569 # # BtgZI: # [GCGATG] # 1093 2417 # # BtsI: # [GCAGTG] # 448 3062 # # Cac8I: # [GCNNGC] # 92 743 786 837 1052 # # Cfr10I: # [RCCGGY] # 31 669 2810 # # CfrI: # [YGGCCR] # 628 2882 # # ClaI: # [ATCGAT] # 242 3268 # # Csp6I: # [GTAC] # 6 9 33 56 71 72 # 81 81 81 81 106 108 # 108 108 108 135 147 175 # 187 204 257 522 774 # # CviAII: # [CATG] # 43 174 177 184 257 272 # 393 409 609 992 # # CviJI: # [RGCY] # 8 10 12 18 19 20 # 20 25 25 27 30 30 # 30 30 38 38 43 46 # 47 51 53 53 54 73 # 76 78 78 78 78 80 # 81 81 81 81 82 85 # 87 95 106 129 139 151 # 165 165 177 241 296 # # CviRI*: # [TGCA] # 21 38 68 81 81 81 # 81 108 108 108 108 121 # 199 216 465 1626 # # DdeI: # [CTNAG] # 84 108 119 120 149 163 # 189 202 240 386 402 633 # 715 # # DpnI: # [GATC] # 27 111 278 413 469 643 # 1569 # # DsaI*: # [CCRYGG] # 173 476 631 2230 # # Eco31I: # [GGTCTC] # 81 189 189 270 940 1841 # # Eco47III: # [AGCGCT] # 315 3195 # # Eco57I: # [CTGAAG] # 312 745 2453 # # Eco57MI: # [CTGRAG] # 116 312 334 745 2003 # # EcoP15I: # [CAGCAG] # 189 189 189 285 353 841 # 1464 # # EcoRI: # [GAATTC] # 1352 2158 # # EcoRII: # [CCWGG] # 81 165 270 320 378 425 # 1871 # # EspI*: # [GCTNAGC] # 872 2638 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 82 994 2370 # # FatI: # [CATG] # 43 174 177 184 256 272 # 393 410 609 992 # # FnuDII*: # [CGCG] # 233 3277 # # FokI: # [GGATG] # 126 180 219 231 238 243 # 318 723 1232 # # GlaI: # [GCGC] # 48 105 267 840 2250 # # GsuI: # [CTGGAG] # 116 334 3060 # # HaeII: # [RGCGCY] # 105 317 838 2250 # # HaeIII: # [GGCC] # 35 146 626 762 1941 # # HgaI: # [GACGC] # 244 3266 # # HgiAI*: # [GWGCWC] # 81 81 81 81 108 108 # 108 108 1024 1730 # # HgiCI*: # [GGYRCC] # 514 583 2413 # # HgiJII*: # [GRGCYC] # 401 497 2612 # # HhaI: # [GCGC] # 48 105 268 839 2250 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 32 # 32 32 32 32 49 157 # 157 238 297 423 482 526 # 925 # # Hin4II*: # [CCTTC] # 515 802 817 1376 # # Hin6I: # [GCGC] # 48 105 266 841 2250 # # HindII: # [GTYRAC] # 686 2824 # # HindIII: # [AAGCTT] # 232 3278 # # HinfI: # [GANTC] # 9 9 33 33 42 80 # 81 81 81 81 81 98 # 108 108 108 108 135 135 # 144 148 162 171 271 301 # 405 497 # # HpaII: # [CCGG] # 32 669 769 2040 # # HphI: # [GGTGA] # 478 567 578 834 1053 # # Hpy166II: # [GTNNAC] # 15 15 15 15 15 15 # 15 15 15 15 33 33 # 33 33 60 60 60 60 # 66 66 66 66 66 91 # 255 595 1717 # # Hpy178III*: # [TCNNGA] # 11 226 758 2515 # # Hpy188I: # [TCNGA] # 9 9 10 18 27 39 # 45 54 69 78 102 111 # 123 192 201 243 252 273 # 1655 # # Hpy99I: # [CGWCG] # 378 1080 2052 # # KpnI: # [GGTACC] # 518 2992 # # Ksp632I*: # [CTCTTC] # 81 189 189 189 864 993 # 1005 # # MaeI: # [CTAG] # 21 24 24 33 57 57 # 57 57 67 81 108 108 # 109 132 132 219 231 274 # 803 916 # # MaeII: # [ACGT] # 77 144 218 393 1185 1493 # # MaeIII: # [GTNAC] # 11 12 19 40 63 69 # 69 69 69 81 84 120 # 120 120 156 180 213 246 # 263 282 318 435 471 # # MboI: # [GATC] # 27 113 278 411 469 643 # 1569 # # MboII: # [GAAGA] # 27 33 48 51 51 54 # 81 81 87 114 189 189 # 189 192 254 260 279 279 # 521 531 # # MluI: # [ACGCGT] # 235 3275 # # MlyI: # [GAGTC] # 42 81 81 81 81 81 # 108 108 108 108 171 421 # 488 590 961 # # MmeI: # [TCCRAC] # 260 546 1281 1423 # # MnlI: # [CCTC] # 6 6 15 18 22 23 # 27 27 33 36 43 53 # 55 60 72 74 93 129 # 140 162 162 166 189 196 # 204 207 232 273 297 490 # # MseI: # [TTAA] # 3 9 142 150 205 288 # 371 557 576 1209 # # MslI: # [CAYNNNNRTG] # 746 2764 # # MwoI: # [GCNNNNNNNGC] # 33 61 76 120 124 147 # 170 185 428 2166 # # NcoI: # [CCATGG] # 649 2861 # # NdeI: # [CATATG] # 81 81 81 81 81 108 # 108 108 108 975 1698 # # NlaIII: # [CATG] # 43 174 177 184 260 272 # 393 406 609 992 # # NlaIV: # [GGNNCC] # 177 404 516 2413 # # NmeAIII: # [GCCGAG] # 97 1315 2098 # # NspBII*: # [CMGCKG] # 94 3416 # # NspI: # [RCATGY] # 184 696 2630 # # OliI: # [CACNNNNGTG] # 746 2764 # # PflMI: # [CCANNNNNTGG] # 249 262 278 2721 # # PleI: # [GAGTC] # 42 81 81 81 81 81 # 108 108 108 108 171 420 # 489 591 960 # # PshAI: # [GACNNNNGTC] # 1115 2395 # # PvuII: # [CAGCTG] # 94 3416 # # RsaI: # [GTAC] # 6 9 33 56 72 72 # 81 81 81 81 105 108 # 108 108 108 135 147 175 # 187 204 257 522 774 # # SapI: # [GCTCTTC] # 81 189 189 189 993 1869 # # ScaI: # [AGTACT] # 1536 1974 # # ScrFI: # [CCNGG] # 81 165 270 322 378 425 # 1869 # # SduI: # [GDGCHC] # 81 81 81 81 108 108 # 108 108 177 320 401 832 # 1024 # # SecI*: # [CCNNGG] # 78 97 156 173 237 320 # 525 553 1371 # # SetI: # [ASST] # 4 12 15 17 19 21 # 21 21 21 22 24 25 # 27 28 30 30 30 30 # 37 41 53 55 57 57 # 60 60 60 60 70 76 # 77 78 78 78 78 78 # 90 92 96 103 112 139 # 140 144 151 153 158 197 # 385 # # SfaNI: # [GCATC] # 11 91 186 363 1042 1817 # # SfeI*: # [CTRYAG] # 24 50 78 267 570 640 # 1881 # # SmlI: # [CTYRAG] # 648 842 2020 # # SphI: # [GCATGC] # 880 2630 # # SspI: # [AATATT] # 136 1174 2200 # # StyI: # [CCWWGG] # 649 868 1993 # # TaiI: # [ACGT] # 77 144 218 396 1185 1490 # # TaqI: # [TCGA] # 15 91 94 227 364 572 # 2147 # # TaqII: # [CACCCA] # 81 189 189 270 477 487 # 1817 # # TatI: # [WGTACW] # 81 81 81 81 107 108 # 108 108 189 204 657 774 # 931 # # TauI: # [GCSGC] # 189 189 189 1162 1781 # # TfiI: # [GAWTC] # 9 9 33 33 98 135 # 148 162 301 351 674 1557 # # TseI: # [GCWGC] # 10 189 189 189 274 326 # 338 411 735 849 # # TsoI: # [TARCCA] # 39 176 1400 1895 # # Tsp45I: # [GTSAC] # 12 69 69 69 69 81 # 120 120 120 180 768 867 # 966 # # Tsp4CI*: # [ACNGT] # 18 24 36 42 57 63 # 72 99 100 109 141 177 # 192 213 224 420 477 1046 # # TspDTI: # [ATGAA] # 28 42 61 66 72 108 # 140 223 310 342 426 1692 # # TspEI: # [AATT] # 18 28 56 65 168 223 # 294 313 420 554 1371 # # TspGWI: # [ACGGA] # 158 209 265 430 479 551 # 1418 # # TspRI: # [CASTGNN] # 39 71 81 81 81 81 # 81 108 108 108 108 147 # 153 276 338 441 518 690 # # TstI: # [CACNNNNNNTCC] # 32 32 32 32 32 157 # 157 157 157 1035 1687 # # Tth111I: # [GACNNNGTC] # 81 189 189 270 951 1830 # # XcmI: # [CCANNNNNNNNNTGG] # 474 1401 1635 # # XhoII: # [RGATCY] # 113 438 2959 # # ZraI: # [GACGTC] # 1636 1874 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3510 # Reported_sequences: 1 # Reported_hitcount: 1006 #---------------------------------------