Restriction Fragments for DAD2/YKR083C



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The currently selected gene/sequence is :

DAD2/YKR083C on chromosome XI from coordinates 596822 to 596421.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.32020.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TfiI GAWTC 4 7 . . 4 8 + HinfI GANTC 4 7 . . 14 18 + TspDTI ATGAA 29 27 . . 21 24 + TspEI AATT 20 24 . . 43 48 - Eco57MI CTGRAG 28 26 . . 43 48 - Eco57I CTGAAG 28 26 . . 62 67 + TsoI TARCCA 78 76 . . 65 69 - BsrI ACTGG 65 63 . . 70 73 + MseI TTAA 70 72 . . 73 77 + TspGWI ACGGA 88 86 . . 75 79 + FokI GGATG 88 92 . . 75 79 + BseGI GGATG 81 79 . . 79 82 + CviJI RGCY 80 80 . . 76 80 - BccI CCATC 70 71 . . 82 85 + MseI TTAA 82 84 . . 86 91 + ApoI RAATTY 86 90 . . 87 90 + TspEI AATT 86 90 . . 92 95 + SetI ASST 95 91 . . 92 95 + CviJI RGCY 93 93 . . 92 95 + AluI AGCT 93 93 . . 114 117 + DpnI GATC 115 115 . . 114 117 + BstKTI GATC 116 114 . . 114 117 + MboI GATC 113 117 . . 130 134 + TspDTI ATGAA 145 143 . . 132 137 + BsmI GAATGC 138 136 . . 139 143 + TfiI GAWTC 139 142 . . 139 143 + HinfI GANTC 139 142 . . 136 139 - AciI CCGC 136 138 . . 143 149 + TspRI CASTGNN 149 140 . . 147 150 + CviJI RGCY 148 148 . . 147 150 + HaeIII GGCC 148 148 . . 147 151 + BmgT120I GGNCC 148 149 . . 147 151 + AsuI* GGNCC 147 150 . . 151 156 + MfeI CAATTG 151 155 . . 152 155 + TspEI AATT 151 155 . . 157 161 + TspDTI ATGAA 172 170 . . 162 167 + MfeI CAATTG 162 166 . . 163 166 + TspEI AATT 162 166 . . 169 173 + TfiI GAWTC 169 172 . . 169 173 + HinfI GANTC 169 172 . . 192 197 + Cac8I GCNNGC 194 194 . . 212 215 + CviRI* TGCA 213 213 . . 219 222 + CviRI* TGCA 220 220 . . 223 226 - MnlI CCTC 216 215 . . 226 230 + HphI GGTGA 238 237 . . 241 245 + BmgT120I GGNCC 242 243 . . 241 245 + AvaII GGWCC 241 244 . . 241 245 + AsuI* GGNCC 241 244 . . 243 247 + Tsp4CI* ACNGT 245 244 . . 244 249 + DsaI* CCRYGG 244 248 . . 244 249 + SecI* CCNNGG 244 248 . . 253 257 + TfiI GAWTC 253 256 . . 253 257 + HinfI GANTC 253 256 . . 257 261 + DdeI CTNAG 257 260 . . 268 272 + TfiI GAWTC 268 271 . . 268 272 + HinfI GANTC 268 271 . . 271 275 + Hpy188I TCNGA 273 272 . . 284 288 + TspDTI ATGAA 299 297 . . 291 294 + SetI ASST 294 290 . . 294 299 + Hpy178III* TCNNGA 295 297 . . 301 304 + Hin6I GCGC 301 303 . . 301 304 + GlaI GCGC 302 302 . . 301 304 + HhaI GCGC 303 301 . . 323 327 + TspDTI ATGAA 338 336 . . 325 329 + MboII GAAGA 337 336 . . 339 343 + FokI GGATG 352 356 . . 339 343 + BseGI GGATG 345 343 . . 337 340 - MnlI CCTC 330 329 . . 340 349 + BsaBI GATNNNNATC 344 344 . . 341 345 + TspDTI ATGAA 356 354 . . 343 347 + MboII GAAGA 355 354 . . 343 355 + BarI GAAGNNNNNNTAC 335 330 367 362 345 350 + XhoII RGATCY 345 349 . . 345 350 + BglII AGATCT 345 349 . . 346 349 + DpnI GATC 347 347 . . 346 349 + BstKTI GATC 348 346 . . 346 349 + MboI GATC 345 349 . . 351 356 + KpnI GGTACC 355 351 . . 351 356 + NlaIV GGNNCC 353 353 . . 351 356 + HgiCI* GGYRCC 351 355 . . 351 356 + Acc65I GGTACC 351 355 . . 352 355 + Csp6I GTAC 352 354 . . 352 355 + RsaI GTAC 353 353 . . 361 364 + HpaII CCGG 361 363 . . 374 378 + Hpy188I TCNGA 376 375 . . 378 381 + TspEI AATT 377 381 . . 392 396 + MaeIII GTNAC 391 396 . . 388 392 - BccI CCATC 382 383 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 51 351 # # AciI: # [CCGC] # 136 266 # # AluI: # [AGCT] # 93 309 # # ApoI: # [RAATTY] # 86 316 # # AsuI*: # [GGNCC] # 94 147 161 # # AvaII: # [GGWCC] # 161 241 # # BarI: # [GAAGNNNNNNTAC] # 32 35 335 # # BccI: # [CCATC] # 20 70 312 # # BglII: # [AGATCT] # 57 345 # # BmgT120I: # [GGNCC] # 94 148 160 # # BsaBI: # [GATNNNNATC] # 58 344 # # BseGI: # [GGATG] # 57 81 264 # # BsmI: # [GAATGC] # 138 264 # # BsrI: # [ACTGG] # 65 337 # # BstKTI: # [GATC] # 54 116 232 # # Cac8I: # [GCNNGC] # 194 208 # # Csp6I: # [GTAC] # 50 352 # # CviJI: # [RGCY] # 13 55 80 254 # # CviRI*: # [TGCA] # 7 182 213 # # DdeI: # [CTNAG] # 145 257 # # DpnI: # [GATC] # 55 115 232 # # DsaI*: # [CCRYGG] # 158 244 # # Eco57I: # [CTGAAG] # 28 374 # # Eco57MI: # [CTGRAG] # 28 374 # # FokI: # [GGATG] # 50 88 264 # # GlaI: # [GCGC] # 100 302 # # HaeIII: # [GGCC] # 148 254 # # HgiCI*: # [GGYRCC] # 51 351 # # HhaI: # [GCGC] # 99 303 # # Hin6I: # [GCGC] # 101 301 # # HinfI: # [GANTC] # 4 15 30 84 134 135 # # HpaII: # [CCGG] # 41 361 # # HphI: # [GGTGA] # 164 238 # # Hpy178III*: # [TCNNGA] # 107 295 # # Hpy188I: # [TCNGA] # 26 103 273 # # KpnI: # [GGTACC] # 47 355 # # MaeIII: # [GTNAC] # 11 391 # # MboI: # [GATC] # 57 113 232 # # MboII: # [GAAGA] # 18 47 337 # # MfeI: # [CAATTG] # 11 151 240 # # MnlI: # [CCTC] # 72 114 216 # # MseI: # [TTAA] # 12 70 320 # # NlaIV: # [GGNNCC] # 49 353 # # RsaI: # [GTAC] # 49 353 # # SecI*: # [CCNNGG] # 158 244 # # SetI: # [ASST] # 95 108 199 # # TfiI: # [GAWTC] # 4 15 30 84 134 135 # # TsoI: # [TARCCA] # 78 324 # # Tsp4CI*: # [ACNGT] # 157 245 # # TspDTI: # [ATGAA] # 18 27 29 39 46 116 # 127 # # TspEI: # [AATT] # 11 20 25 65 66 215 # # TspGWI: # [ACGGA] # 88 314 # # TspRI: # [CASTGNN] # 149 253 # # XhoII: # [RGATCY] # 57 345 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 402 # Reported_sequences: 1 # Reported_hitcount: 88 #---------------------------------------