Restriction Fragments for NUP133/YKR082W



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The currently selected gene/sequence is :

NUP133/YKR082W on chromosome XI from coordinates 592825 to 596298.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2412.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 20 + TatI WGTACW 15 19 . . 16 19 + Csp6I GTAC 16 18 . . 16 19 + RsaI GTAC 17 17 . . 14 24 - FalI AAGNNNNNCTT 37 32 5 0 21 25 - MboII GAAGA 13 12 . . 30 33 - AciI CCGC 30 32 . . 37 49 + PsrI GAACNNNNNNTAC 29 24 61 56 33 37 - Hin4II* CCTTC 27 26 . . 40 44 + DdeI CTNAG 40 43 . . 46 49 + Csp6I GTAC 46 48 . . 46 49 + RsaI GTAC 47 47 . . 50 61 + BstXI CCANNNNNNTGG 57 53 . . 51 60 + MslI CAYNNNNRTG 55 55 . . 51 56 - BsrDI GCAATG 50 48 . . 55 58 - AciI CCGC 55 57 . . 70 74 + TfiI GAWTC 70 73 . . 70 74 + HinfI GANTC 70 73 . . 74 79 + SecI* CCNNGG 74 78 . . 75 79 + BssKI CCNGG 74 79 . . 75 79 + BseBI CCWGG 76 77 . . 75 79 + ScrFI CCNGG 76 77 . . 75 79 + EcoRII CCWGG 74 79 . . 79 82 + Hin6I GCGC 79 81 . . 79 82 + GlaI GCGC 80 80 . . 79 82 + HhaI GCGC 81 79 . . 92 96 + TspDTI ATGAA 107 105 . . 94 98 + MboII GAAGA 106 105 . . 94 99 - Ksp632I* CTCTTC 89 92 . . 97 100 - MnlI CCTC 90 89 . . 103 106 + CviJI RGCY 104 104 . . 105 109 + BssKI CCNGG 104 109 . . 105 109 + BseBI CCWGG 106 107 . . 105 109 + ScrFI CCNGG 106 107 . . 105 109 + EcoRII CCWGG 104 109 . . 107 112 + StuI AGGCCT 109 109 . . 108 111 + CviJI RGCY 109 109 . . 108 111 + HaeIII GGCC 109 109 . . 110 113 + MnlI CCTC 120 119 . . 123 132 + MslI CAYNNNNRTG 127 127 . . 128 133 + DsaI* CCRYGG 128 132 . . 128 133 + StyI CCWWGG 128 132 . . 128 133 + NcoI CCATGG 128 132 . . 128 133 + SecI* CCNNGG 128 132 . . 129 132 + FatI CATG 128 132 . . 129 132 + NlaIII CATG 132 128 . . 129 132 + CviAII CATG 129 131 . . 135 139 + BlsI GCNGC 137 136 . . 135 139 + BisI GCNGC 136 137 . . 135 139 + BbvI GCAGC 147 151 . . 135 139 + TseI GCWGC 135 138 . . 141 145 + Tsp4CI* ACNGT 143 142 . . 145 148 + MseI TTAA 145 147 . . 148 151 + SetI ASST 151 147 . . 150 154 + MaeIII GTNAC 149 154 . . 169 172 + TspEI AATT 168 172 . . 173 178 + StyI CCWWGG 173 177 . . 173 178 + SecI* CCNNGG 173 177 . . 176 179 + SetI ASST 179 175 . . 213 217 + Hin4II* CCTTC 223 222 . . 205 217 - BarI GAAGNNNNNNTAC 229 224 197 192 225 230 + SfeI* CTRYAG 225 229 . . 227 231 + Tsp4CI* ACNGT 229 228 . . 236 240 + TfiI GAWTC 236 239 . . 236 240 + HinfI GANTC 236 239 . . 233 236 - AciI CCGC 233 235 . . 242 246 + MaeIII GTNAC 241 246 . . 254 257 + TspEI AATT 253 257 . . 268 273 + ClaI ATCGAT 269 271 . . 269 272 + TaqI TCGA 269 271 . . 275 278 + CviRI* TGCA 276 276 . . 279 282 + TspEI AATT 278 282 . . 291 296 + SpeI ACTAGT 291 295 . . 292 295 + MaeI CTAG 292 294 . . 303 313 + BsiYI* CCNNNNNNNGG 309 306 . . 311 314 + SetI ASST 314 310 . . 309 313 - Hin4II* CCTTC 303 302 . . 313 318 + Hpy166II GTNNAC 315 315 . . 320 325 + Hpy178III* TCNNGA 321 323 . . 325 330 + SspI AATATT 327 327 . . 345 350 - BciVI GTATCC 339 338 . . 361 365 + Tsp4CI* ACNGT 363 362 . . 364 367 + Csp6I GTAC 364 366 . . 364 367 + RsaI GTAC 365 365 . . 376 380 + Hpy188I TCNGA 378 377 . . 389 393 + TspDTI ATGAA 404 402 . . 402 405 + FnuDII* CGCG 403 403 . . 403 406 + Hin6I GCGC 403 405 . . 403 406 + GlaI GCGC 404 404 . . 403 406 + HhaI GCGC 405 403 . . 406 411 + SecI* CCNNGG 406 410 . . 407 411 + BssKI CCNGG 406 411 . . 407 411 + BseBI CCWGG 408 409 . . 407 411 + ScrFI CCNGG 408 409 . . 407 411 + EcoRII CCWGG 406 411 . . 409 412 + SetI ASST 412 408 . . 412 415 + CviRI* TGCA 413 413 . . 438 442 + FokI GGATG 451 455 . . 438 442 + BseGI GGATG 444 442 . . 442 446 + PleI GAGTC 450 451 . . 442 446 + MlyI GAGTC 451 451 . . 442 446 + HinfI GANTC 442 445 . . 444 448 + BsmAI GTCTC 449 453 . . 455 461 + TspRI CASTGNN 461 452 . . 459 463 + TfiI GAWTC 459 462 . . 459 463 + HinfI GANTC 459 462 . . 468 473 + BclI TGATCA 468 472 . . 469 472 + DpnI GATC 470 470 . . 469 472 + BstKTI GATC 471 469 . . 469 472 + MboI GATC 468 472 . . 471 476 + Hpy178III* TCNNGA 472 474 . . 481 486 + BetI* WCCGGW 481 485 . . 482 485 + HpaII CCGG 482 484 . . 485 488 - MnlI CCTC 478 477 . . 488 491 + CviJI RGCY 489 489 . . 485 495 - Hin4I GAYNNNNNVTC 508 503 476 471 504 507 + SetI ASST 507 503 . . 526 529 - MnlI CCTC 519 518 . . 529 539 + Hin4I GAYNNNNNVTC 520 515 552 547 529 534 + EcoRV GATATC 531 531 . . 547 550 + TspEI AATT 546 550 . . 557 560 + MseI TTAA 557 559 . . 565 569 + Hpy188I TCNGA 567 566 . . 571 576 + ApoI RAATTY 571 575 . . 572 575 + TspEI AATT 571 575 . . 579 584 + Hpy178III* TCNNGA 580 582 . . 580 585 + BsiI* CACGAG 580 584 . . 590 593 + TspEI AATT 589 593 . . 596 600 + BccI CCATC 604 605 . . 603 608 + Ksp632I* CTCTTC 609 612 . . 604 608 - MboII GAAGA 596 595 . . 610 615 + EciI GGCGGA 626 624 . . 611 614 - AciI CCGC 611 613 . . 623 627 + Tsp45I GTSAC 622 627 . . 623 627 + MaeIII GTNAC 622 627 . . 626 629 + SetI ASST 629 625 . . 634 637 + MseI TTAA 634 636 . . 645 648 + SetI ASST 648 644 . . 645 650 + BspMI ACCTGC 654 658 . . 647 652 - EcoP15I CAGCAG 619 621 . . 681 686 + SspI AATATT 683 683 . . 689 694 + PsiI TTATAA 691 691 . . 692 697 + TsoI TARCCA 708 706 . . 702 707 + ApoI RAATTY 702 706 . . 703 706 + TspEI AATT 702 706 . . 707 712 + DsaI* CCRYGG 707 711 . . 707 712 + StyI CCWWGG 707 711 . . 707 712 + NcoI CCATGG 707 711 . . 707 712 + SecI* CCNNGG 707 711 . . 708 711 + FatI CATG 707 711 . . 708 711 + NlaIII CATG 711 707 . . 708 711 + CviAII CATG 708 710 . . 713 718 + Hpy166II GTNNAC 715 715 . . 717 720 + SetI ASST 720 716 . . 718 721 + MnlI CCTC 728 727 . . 724 729 + Eco57MI CTGRAG 745 743 . . 724 729 + Eco57I CTGAAG 745 743 . . 742 745 + MseI TTAA 742 744 . . 750 753 + SetI ASST 753 749 . . 758 761 + TspEI AATT 757 761 . . 770 774 + BmgT120I GGNCC 771 772 . . 770 774 + AvaII GGWCC 770 773 . . 770 774 + AsuI* GGNCC 770 773 . . 776 781 + ApoI RAATTY 776 780 . . 777 780 + TspEI AATT 776 780 . . 791 795 + Tsp4CI* ACNGT 793 792 . . 802 806 + BsmAI GTCTC 807 811 . . 817 821 + BmgT120I GGNCC 818 819 . . 817 821 + AvaII GGWCC 817 820 . . 817 821 + AsuI* GGNCC 817 820 . . 818 828 + Hin4I GAYNNNNNVTC 809 804 841 836 819 822 + SetI ASST 822 818 . . 820 830 + BsiYI* CCNNNNNNNGG 826 823 . . 825 828 + MnlI CCTC 835 834 . . 825 835 + EcoNI CCTNNNNNAGG 829 830 . . 825 830 + SecI* CCNNGG 825 829 . . 825 835 + BsiYI* CCNNNNNNNGG 831 828 . . 835 839 + BslFI GGGAC 849 853 . . 841 844 + SetI ASST 844 840 . . 869 874 + ApoI RAATTY 869 873 . . 870 873 + TspEI AATT 869 873 . . 881 884 + FatI CATG 880 884 . . 881 884 + NlaIII CATG 884 880 . . 881 884 + CviAII CATG 881 883 . . 885 896 + AlfI GCANNNNNNTGC 874 872 908 906 885 896 - AlfI GCANNNNNNTGC 908 906 874 872 900 905 + EcoRI GAATTC 900 904 . . 900 905 + ApoI RAATTY 900 904 . . 901 904 + TspEI AATT 900 904 . . 906 910 + BccI CCATC 914 915 . . 909 914 + MmeI TCCRAC 934 932 . . 907 911 - FokI GGATG 893 897 . . 907 911 - BseGI GGATG 906 904 . . 917 920 + TspEI AATT 916 920 . . 928 933 + Hpy166II GTNNAC 930 930 . . 942 945 + CviJI RGCY 943 943 . . 952 956 + PleI GAGTC 960 961 . . 952 956 + MlyI GAGTC 961 961 . . 952 956 + HinfI GANTC 952 955 . . 959 965 + PfoI TCCNGGA 959 964 . . 960 964 + BssKI CCNGG 959 964 . . 960 964 + BseBI CCWGG 961 962 . . 960 964 + ScrFI CCNGG 961 962 . . 960 964 + EcoRII CCWGG 959 964 . . 958 963 - Eco57MI CTGRAG 943 941 . . 958 963 - GsuI CTGGAG 943 941 . . 964 973 + BsaBI GATNNNNATC 968 968 . . 969 974 + BciVI GTATCC 980 979 . . 982 985 + FatI CATG 981 985 . . 982 985 + NlaIII CATG 985 981 . . 982 985 + CviAII CATG 982 984 . . 986 989 + Csp6I GTAC 986 988 . . 986 989 + RsaI GTAC 987 987 . . 988 991 + TaiI ACGT 991 987 . . 988 991 + SetI ASST 991 987 . . 988 991 + MaeII ACGT 988 990 . . 993 997 + MboII GAAGA 1005 1004 . . 1001 1005 + BslFI GGGAC 1015 1019 . . 1003 1007 + HinfI GANTC 1003 1006 . . 1003 1007 - PleI GAGTC 997 998 . . 1003 1007 - MlyI GAGTC 997 997 . . 1009 1013 - FokI GGATG 995 999 . . 1009 1013 - BseGI GGATG 1008 1006 . . 1022 1026 + TspDTI ATGAA 1037 1035 . . 1028 1033 + Hpy166II GTNNAC 1030 1030 . . 1031 1042 + TstI CACNNNNNNTCC 1022 1017 1054 1049 1058 1062 + Tsp4CI* ACNGT 1060 1059 . . 1064 1067 + FatI CATG 1063 1067 . . 1064 1067 + NlaIII CATG 1067 1063 . . 1064 1067 + CviAII CATG 1064 1066 . . 1062 1066 - TspDTI ATGAA 1052 1050 . . 1066 1069 + CviRI* TGCA 1067 1067 . . 1067 1072 + BsrDI GCAATG 1074 1072 . . 1070 1074 + TspDTI ATGAA 1085 1083 . . 1103 1106 + TaqI TCGA 1103 1105 . . 1100 1104 - MboII GAAGA 1092 1091 . . 1105 1109 + TfiI GAWTC 1105 1108 . . 1105 1109 + HinfI GANTC 1105 1108 . . 1108 1112 - MboII GAAGA 1100 1099 . . 1111 1115 - MboII GAAGA 1103 1102 . . 1152 1161 + MslI CAYNNNNRTG 1156 1156 . . 1162 1166 + PleI GAGTC 1170 1171 . . 1162 1166 + MlyI GAGTC 1171 1171 . . 1162 1166 + HinfI GANTC 1162 1165 . . 1184 1187 + MseI TTAA 1184 1186 . . 1187 1190 + CviJI RGCY 1188 1188 . . 1190 1194 + DdeI CTNAG 1190 1193 . . 1203 1206 + SetI ASST 1206 1202 . . 1204 1207 + MnlI CCTC 1214 1213 . . 1205 1209 + DdeI CTNAG 1205 1208 . . 1205 1209 + BspCNI CTCAG 1218 1216 . . 1205 1209 + BseMII CTCAG 1219 1217 . . 1206 1210 + Hpy188I TCNGA 1208 1207 . . 1209 1213 - BccI CCATC 1203 1204 . . 1215 1220 + BsmI GAATGC 1221 1219 . . 1235 1238 + SetI ASST 1238 1234 . . 1234 1237 - MnlI CCTC 1227 1226 . . 1237 1241 + MaeIII GTNAC 1236 1241 . . 1280 1284 + DdeI CTNAG 1280 1283 . . 1280 1284 + BspCNI CTCAG 1293 1291 . . 1280 1284 + BseMII CTCAG 1294 1292 . . 1283 1286 + SetI ASST 1286 1282 . . 1284 1288 + HphI GGTGA 1296 1295 . . 1285 1290 + Hpy166II GTNNAC 1287 1287 . . 1287 1300 + AjuI GAANNNNNNNTTGG 1279 1274 1311 1306 1295 1298 + TspEI AATT 1294 1298 . . 1300 1304 + HinfI GANTC 1300 1303 . . 1300 1304 - PleI GAGTC 1294 1295 . . 1300 1304 - MlyI GAGTC 1294 1294 . . 1304 1307 + MaeI CTAG 1304 1306 . . 1306 1309 + SetI ASST 1309 1305 . . 1306 1309 + CviJI RGCY 1307 1307 . . 1306 1309 + AluI AGCT 1307 1307 . . 1319 1323 + MboII GAAGA 1331 1330 . . 1330 1334 + MboII GAAGA 1342 1341 . . 1342 1345 + CviJI RGCY 1343 1343 . . 1345 1349 + DdeI CTNAG 1345 1348 . . 1365 1368 + TspEI AATT 1364 1368 . . 1376 1380 + MaeIII GTNAC 1375 1380 . . 1374 1379 - TsoI TARCCA 1364 1362 . . 1381 1385 + HinfI GANTC 1381 1384 . . 1381 1391 + Hin4I GAYNNNNNVTC 1372 1367 1404 1399 1381 1385 - PleI GAGTC 1375 1376 . . 1381 1385 - MlyI GAGTC 1375 1375 . . 1381 1391 - Hin4I GAYNNNNNVTC 1404 1399 1372 1367 1402 1405 + MseI TTAA 1402 1404 . . 1423 1428 + SfeI* CTRYAG 1423 1427 . . 1425 1429 + Tsp4CI* ACNGT 1427 1426 . . 1447 1450 - MnlI CCTC 1440 1439 . . 1455 1460 + ApoI RAATTY 1455 1459 . . 1456 1459 + TspEI AATT 1455 1459 . . 1464 1469 + BetI* WCCGGW 1464 1468 . . 1465 1468 + HpaII CCGG 1465 1467 . . 1465 1475 + BsiYI* CCNNNNNNNGG 1471 1468 . . 1489 1492 + FatI CATG 1488 1492 . . 1489 1492 + NlaIII CATG 1492 1488 . . 1489 1492 + CviAII CATG 1489 1491 . . 1494 1499 + BclI TGATCA 1494 1498 . . 1495 1498 + DpnI GATC 1496 1496 . . 1495 1498 + BstKTI GATC 1497 1495 . . 1495 1498 + MboI GATC 1494 1498 . . 1500 1503 + SetI ASST 1503 1499 . . 1500 1503 + CviJI RGCY 1501 1501 . . 1500 1503 + AluI AGCT 1501 1501 . . 1524 1527 + CviRI* TGCA 1525 1525 . . 1543 1546 + TspEI AATT 1542 1546 . . 1548 1551 + SetI ASST 1551 1547 . . 1549 1552 + MnlI CCTC 1559 1558 . . 1551 1556 + Hpy178III* TCNNGA 1552 1554 . . 1550 1555 - Eco57MI CTGRAG 1535 1533 . . 1550 1555 - GsuI CTGGAG 1535 1533 . . 1556 1561 + ApoI RAATTY 1556 1560 . . 1557 1560 + TspEI AATT 1556 1560 . . 1564 1567 + MaeI CTAG 1564 1566 . . 1567 1577 + Hin4I GAYNNNNNVTC 1558 1553 1590 1585 1567 1570 + DpnI GATC 1568 1568 . . 1567 1570 + BstKTI GATC 1569 1567 . . 1567 1570 + MboI GATC 1566 1570 . . 1569 1574 + Hpy178III* TCNNGA 1570 1572 . . 1573 1577 + TfiI GAWTC 1573 1576 . . 1573 1577 + HinfI GANTC 1573 1576 . . 1567 1577 - Hin4I GAYNNNNNVTC 1590 1585 1558 1553 1577 1582 + SfeI* CTRYAG 1577 1581 . . 1585 1588 + FatI CATG 1584 1588 . . 1585 1588 + NlaIII CATG 1588 1584 . . 1585 1588 + CviAII CATG 1585 1587 . . 1590 1595 + AhaIII* TTTAAA 1592 1592 . . 1591 1594 + MseI TTAA 1591 1593 . . 1595 1598 + TspEI AATT 1594 1598 . . 1597 1600 + MseI TTAA 1597 1599 . . 1598 1603 + TsoI TARCCA 1614 1612 . . 1606 1611 + BseRI GAGGAG 1621 1619 . . 1606 1609 - MnlI CCTC 1599 1598 . . 1616 1620 - TspDTI ATGAA 1606 1604 . . 1621 1626 + MmeI TCCRAC 1646 1644 . . 1625 1629 + BceAI ACGGC 1641 1643 . . 1631 1636 + TatI WGTACW 1631 1635 . . 1632 1635 + Csp6I GTAC 1632 1634 . . 1632 1635 + RsaI GTAC 1633 1633 . . 1654 1657 + TaiI ACGT 1657 1653 . . 1654 1657 + SetI ASST 1657 1653 . . 1654 1657 + MaeII ACGT 1654 1656 . . 1676 1680 - TspGWI ACGGA 1666 1664 . . 1684 1693 + XmnI GAANNNNTTC 1688 1688 . . 1684 1689 + ApoI RAATTY 1684 1688 . . 1685 1688 + TspEI AATT 1684 1688 . . 1695 1700 + ApoI RAATTY 1695 1699 . . 1696 1699 + TspEI AATT 1695 1699 . . 1700 1705 + Hpy178III* TCNNGA 1701 1703 . . 1715 1719 + DdeI CTNAG 1715 1718 . . 1738 1742 - HphI GGTGA 1730 1729 . . 1751 1754 + TspEI AATT 1750 1754 . . 1755 1760 + XhoII RGATCY 1755 1759 . . 1755 1759 + BinI* GGATC 1763 1764 . . 1756 1759 + DpnI GATC 1757 1757 . . 1756 1759 + BstKTI GATC 1758 1756 . . 1756 1759 + MboI GATC 1755 1759 . . 1758 1762 + Hpy188I TCNGA 1760 1759 . . 1761 1764 + DpnI GATC 1762 1762 . . 1761 1764 + BstKTI GATC 1763 1761 . . 1761 1764 + MboI GATC 1760 1764 . . 1763 1766 + TaqI TCGA 1763 1765 . . 1768 1773 + ApoI RAATTY 1768 1772 . . 1769 1772 + TspEI AATT 1768 1772 . . 1775 1780 + XhoII RGATCY 1775 1779 . . 1775 1780 + BglII AGATCT 1775 1779 . . 1776 1779 + DpnI GATC 1777 1777 . . 1776 1779 + BstKTI GATC 1778 1776 . . 1776 1779 + MboI GATC 1775 1779 . . 1778 1783 + Hpy178III* TCNNGA 1779 1781 . . 1783 1786 + TspEI AATT 1782 1786 . . 1793 1796 + MseI TTAA 1793 1795 . . 1811 1815 + Hpy188I TCNGA 1813 1812 . . 1819 1823 + Hpy188I TCNGA 1821 1820 . . 1845 1849 + MboII GAAGA 1857 1856 . . 1845 1850 + BbvII* GAAGAC 1852 1856 . . 1866 1870 + Hpy188I TCNGA 1868 1867 . . 1877 1881 + TspDTI ATGAA 1892 1890 . . 1885 1888 + MseI TTAA 1885 1887 . . 1897 1901 + Tsp4CI* ACNGT 1899 1898 . . 1910 1914 + Tsp4CI* ACNGT 1912 1911 . . 1914 1919 + Hpy178III* TCNNGA 1915 1917 . . 1917 1921 + Hpy99I CGWCG 1921 1916 . . 1913 1917 - BslFI GGGAC 1898 1902 . . 1919 1922 + TaiI ACGT 1922 1918 . . 1919 1922 + SetI ASST 1922 1918 . . 1919 1922 + MaeII ACGT 1919 1921 . . 1927 1931 - BsrI ACTGG 1927 1925 . . 1936 1941 + AhaIII* TTTAAA 1938 1938 . . 1937 1940 + MseI TTAA 1937 1939 . . 1943 1946 + TspEI AATT 1942 1946 . . 1947 1952 + AhaIII* TTTAAA 1949 1949 . . 1948 1951 + MseI TTAA 1948 1950 . . 1954 1957 + FatI CATG 1953 1957 . . 1954 1957 + NlaIII CATG 1957 1953 . . 1954 1957 + CviAII CATG 1954 1956 . . 1975 1979 + BccI CCATC 1983 1984 . . 1989 1994 + ApoI RAATTY 1989 1993 . . 1990 1993 + TspEI AATT 1989 1993 . . 2005 2009 + MaeIII GTNAC 2004 2009 . . 2011 2014 + TspEI AATT 2010 2014 . . 2014 2017 + MseI TTAA 2014 2016 . . 2039 2044 + EcoRI GAATTC 2039 2043 . . 2035 2039 - BccI CCATC 2029 2030 . . 2039 2044 + ApoI RAATTY 2039 2043 . . 2040 2043 + TspEI AATT 2039 2043 . . 2043 2048 + Hpy178III* TCNNGA 2044 2046 . . 2047 2051 + MboII GAAGA 2059 2058 . . 2052 2055 + SetI ASST 2055 2051 . . 2053 2057 + HphI GGTGA 2065 2064 . . 2050 2054 - Hin4II* CCTTC 2044 2043 . . 2068 2071 + SetI ASST 2071 2067 . . 2072 2076 + TspDTI ATGAA 2087 2085 . . 2091 2101 + BsiYI* CCNNNNNNNGG 2097 2094 . . 2094 2098 - BccI CCATC 2088 2089 . . 2099 2102 + SetI ASST 2102 2098 . . 2098 2101 - MnlI CCTC 2091 2090 . . 2108 2112 - FokI GGATG 2094 2098 . . 2108 2112 - BseGI GGATG 2107 2105 . . 2114 2117 + SetI ASST 2117 2113 . . 2114 2117 + CviJI RGCY 2115 2115 . . 2114 2117 + AluI AGCT 2115 2115 . . 2119 2124 + DsaI* CCRYGG 2119 2123 . . 2119 2124 + StyI CCWWGG 2119 2123 . . 2119 2124 + NcoI CCATGG 2119 2123 . . 2119 2124 + SecI* CCNNGG 2119 2123 . . 2120 2123 + FatI CATG 2119 2123 . . 2120 2123 + NlaIII CATG 2123 2119 . . 2120 2123 + CviAII CATG 2120 2122 . . 2125 2129 - TspDTI ATGAA 2115 2113 . . 2143 2146 + MseI TTAA 2143 2145 . . 2147 2151 + Tsp4CI* ACNGT 2149 2148 . . 2177 2186 + MslI CAYNNNNRTG 2181 2181 . . 2188 2193 + BseRI GAGGAG 2203 2201 . . 2188 2191 - MnlI CCTC 2181 2180 . . 2191 2194 - MnlI CCTC 2184 2183 . . 2194 2198 - Hin4II* CCTTC 2188 2187 . . 2202 2207 + Hpy178III* TCNNGA 2203 2205 . . 2206 2210 + TfiI GAWTC 2206 2209 . . 2206 2210 + HinfI GANTC 2206 2209 . . 2211 2216 + PsiI TTATAA 2213 2213 . . 2218 2221 - MnlI CCTC 2211 2210 . . 2221 2224 + CviJI RGCY 2222 2222 . . 2227 2230 + MseI TTAA 2227 2229 . . 2240 2245 + ApoI RAATTY 2240 2244 . . 2241 2244 + TspEI AATT 2240 2244 . . 2264 2267 + TspEI AATT 2263 2267 . . 2266 2271 + AhaIII* TTTAAA 2268 2268 . . 2267 2270 + MseI TTAA 2267 2269 . . 2276 2280 + BinI* GGATC 2284 2285 . . 2277 2280 + DpnI GATC 2278 2278 . . 2277 2280 + BstKTI GATC 2279 2277 . . 2277 2280 + MboI GATC 2276 2280 . . 2294 2297 + MseI TTAA 2294 2296 . . 2302 2307 + Hpy166II GTNNAC 2304 2304 . . 2317 2320 + TspEI AATT 2316 2320 . . 2321 2326 + PsiI TTATAA 2323 2323 . . 2349 2354 + TsoI TARCCA 2365 2363 . . 2354 2357 + SetI ASST 2357 2353 . . 2355 2359 + BssKI CCNGG 2354 2359 . . 2355 2359 + BseBI CCWGG 2356 2357 . . 2355 2359 + ScrFI CCNGG 2356 2357 . . 2355 2359 + EcoRII CCWGG 2354 2359 . . 2352 2363 - CspCI CAANNNNNGTGG 2376 2374 2341 2339 2363 2368 + NlaIV GGNNCC 2365 2365 . . 2368 2373 + BtrI CACGTC 2370 2370 . . 2369 2372 + TaiI ACGT 2372 2368 . . 2369 2372 + SetI ASST 2372 2368 . . 2369 2372 + MaeII ACGT 2369 2371 . . 2373 2383 + EcoNI CCTNNNNNAGG 2377 2378 . . 2373 2383 + BsiYI* CCNNNNNNNGG 2379 2376 . . 2367 2378 - CspCI CAANNNNNGTGG 2391 2389 2356 2354 2381 2384 + SetI ASST 2384 2380 . . 2397 2401 + Tsp4CI* ACNGT 2399 2398 . . 2398 2404 + TspRI CASTGNN 2404 2395 . . 2416 2421 + ApoI RAATTY 2416 2420 . . 2417 2420 + TspEI AATT 2416 2420 . . 2434 2439 + Hpy178III* TCNNGA 2435 2437 . . 2444 2447 + CviRI* TGCA 2445 2445 . . 2464 2467 + DpnI GATC 2465 2465 . . 2464 2467 + BstKTI GATC 2466 2464 . . 2464 2467 + MboI GATC 2463 2467 . . 2473 2477 + HinfI GANTC 2473 2476 . . 2473 2477 - PleI GAGTC 2467 2468 . . 2473 2477 - MlyI GAGTC 2467 2467 . . 2479 2489 + BaeI ACNNNNGTAYC 2468 2463 2501 2496 2482 2486 + Tsp4CI* ACNGT 2484 2483 . . 2504 2508 - MboII GAAGA 2496 2495 . . 2512 2517 + EcoRI GAATTC 2512 2516 . . 2512 2517 + ApoI RAATTY 2512 2516 . . 2513 2516 + TspEI AATT 2512 2516 . . 2530 2533 + SetI ASST 2533 2529 . . 2530 2533 + CviJI RGCY 2531 2531 . . 2530 2533 + AluI AGCT 2531 2531 . . 2546 2551 + SspI AATATT 2548 2548 . . 2551 2554 + MseI TTAA 2551 2553 . . 2567 2570 + TspEI AATT 2566 2570 . . 2570 2581 + BdaI TGANNNNNNTCA 2559 2557 2593 2591 2576 2579 + SetI ASST 2579 2575 . . 2577 2580 + MnlI CCTC 2587 2586 . . 2570 2581 - BdaI TGANNNNNNTCA 2593 2591 2559 2557 2582 2586 - MboII GAAGA 2574 2573 . . 2587 2590 + SetI ASST 2590 2586 . . 2583 2588 - Eco57MI CTGRAG 2568 2566 . . 2588 2593 + NlaIV GGNNCC 2590 2590 . . 2583 2588 - Eco57I CTGAAG 2568 2566 . . 2611 2614 + MseI TTAA 2611 2613 . . 2616 2620 + Tsp4CI* ACNGT 2618 2617 . . 2629 2633 + PleI GAGTC 2637 2638 . . 2629 2633 + MlyI GAGTC 2638 2638 . . 2629 2633 + HinfI GANTC 2629 2632 . . 2633 2636 + AciI CCGC 2633 2635 . . 2633 2638 + BsrBI CCGCTC 2635 2635 . . 2638 2648 + BsiYI* CCNNNNNNNGG 2644 2641 . . 2643 2646 + Csp6I GTAC 2643 2645 . . 2643 2646 + RsaI GTAC 2644 2644 . . 2645 2649 + Tsp4CI* ACNGT 2647 2646 . . 2650 2653 + FatI CATG 2649 2653 . . 2650 2653 + NlaIII CATG 2653 2649 . . 2650 2653 + CviAII CATG 2650 2652 . . 2657 2660 + FatI CATG 2656 2660 . . 2657 2660 + NlaIII CATG 2660 2656 . . 2657 2660 + CviAII CATG 2657 2659 . . 2660 2665 + XhoII RGATCY 2660 2664 . . 2660 2665 + NlaIV GGNNCC 2662 2662 . . 2660 2665 + BamHI GGATCC 2660 2664 . . 2660 2664 + BinI* GGATC 2668 2669 . . 2661 2664 + DpnI GATC 2662 2662 . . 2661 2664 + BstKTI GATC 2663 2661 . . 2661 2664 + MboI GATC 2660 2664 . . 2663 2668 + MmeI TCCRAC 2688 2686 . . 2661 2665 - BinI* GGATC 2655 2656 . . 2668 2676 + AlwNI CAGNNNCTG 2673 2670 . . 2670 2674 + TfiI GAWTC 2670 2673 . . 2670 2674 + HinfI GANTC 2670 2673 . . 2673 2678 + Hpy178III* TCNNGA 2674 2676 . . 2676 2680 + FokI GGATG 2689 2693 . . 2676 2680 + BseGI GGATG 2682 2680 . . 2677 2681 - BccI CCATC 2671 2672 . . 2684 2689 + SplI* CGTACG 2684 2688 . . 2685 2688 + Csp6I GTAC 2685 2687 . . 2685 2688 + RsaI GTAC 2686 2686 . . 2692 2696 - SfaNI GCATC 2682 2686 . . 2697 2700 + FatI CATG 2696 2700 . . 2697 2700 + NlaIII CATG 2700 2696 . . 2697 2700 + CviAII CATG 2697 2699 . . 2698 2702 + TspDTI ATGAA 2713 2711 . . 2706 2711 + AhaIII* TTTAAA 2708 2708 . . 2707 2710 + MseI TTAA 2707 2709 . . 2719 2723 + Tsp4CI* ACNGT 2721 2720 . . 2728 2732 + TfiI GAWTC 2728 2731 . . 2728 2732 + HinfI GANTC 2728 2731 . . 2736 2740 - Hin4II* CCTTC 2730 2729 . . 2745 2750 + HindIII AAGCTT 2745 2749 . . 2746 2749 + SetI ASST 2749 2745 . . 2746 2749 + CviJI RGCY 2747 2747 . . 2746 2749 + AluI AGCT 2747 2747 . . 2748 2753 + SmlI CTYRAG 2748 2752 . . 2748 2753 + Bce83I* CTTGAG 2769 2767 . . 2762 2765 + TspEI AATT 2761 2765 . . 2765 2768 + CviRI* TGCA 2766 2766 . . 2767 2776 + MslI CAYNNNNRTG 2771 2771 . . 2778 2781 + CviRI* TGCA 2779 2779 . . 2783 2786 + TspEI AATT 2782 2786 . . 2785 2788 + MseI TTAA 2785 2787 . . 2788 2791 + SetI ASST 2791 2787 . . 2788 2791 + CviJI RGCY 2789 2789 . . 2788 2791 + AluI AGCT 2789 2789 . . 2797 2800 + MaeI CTAG 2797 2799 . . 2812 2815 + TspEI AATT 2811 2815 . . 2821 2826 + VspI ATTAAT 2822 2824 . . 2822 2825 + MseI TTAA 2822 2824 . . 2837 2842 + XhoII RGATCY 2837 2841 . . 2838 2841 + DpnI GATC 2839 2839 . . 2838 2841 + BstKTI GATC 2840 2838 . . 2838 2841 + MboI GATC 2837 2841 . . 2838 2842 - BinI* GGATC 2832 2833 . . 2848 2851 + TspEI AATT 2847 2851 . . 2858 2863 + MfeI CAATTG 2858 2862 . . 2859 2862 + TspEI AATT 2858 2862 . . 2863 2867 - SfaNI GCATC 2853 2857 . . 2867 2871 + DdeI CTNAG 2867 2870 . . 2867 2871 - BspCNI CTCAG 2859 2857 . . 2867 2871 - BseMII CTCAG 2858 2856 . . 2875 2880 + SspI AATATT 2877 2877 . . 2888 2891 + TspEI AATT 2887 2891 . . 2907 2911 + Hpy188I TCNGA 2909 2908 . . 2915 2920 + Hpy166II GTNNAC 2917 2917 . . 2924 2929 + Hpy178III* TCNNGA 2925 2927 . . 2932 2935 + CviJI RGCY 2933 2933 . . 2955 2964 + MslI CAYNNNNRTG 2959 2959 . . 2965 2969 + MboII GAAGA 2977 2976 . . 2969 2972 + SetI ASST 2972 2968 . . 2969 2972 + CviJI RGCY 2970 2970 . . 2969 2972 + AluI AGCT 2970 2970 . . 2965 2970 - Ksp632I* CTCTTC 2960 2963 . . 2965 2971 - SapI GCTCTTC 2960 2963 . . 2972 2975 + MseI TTAA 2972 2974 . . 2968 2978 - Hin4I GAYNNNNNVTC 2991 2986 2959 2954 2983 2987 + Tsp4CI* ACNGT 2985 2984 . . 2998 3002 + Hpy188I TCNGA 3000 2999 . . 3000 3005 + XhoII RGATCY 3000 3004 . . 3000 3004 + BinI* GGATC 3008 3009 . . 3001 3004 + DpnI GATC 3002 3002 . . 3001 3004 + BstKTI GATC 3003 3001 . . 3001 3004 + MboI GATC 3000 3004 . . 3026 3029 + TaqI TCGA 3026 3028 . . 3027 3031 + Hpy99I CGWCG 3031 3026 . . 3032 3036 + TspDTI ATGAA 3047 3045 . . 3034 3038 + MboII GAAGA 3046 3045 . . 3034 3039 - Ksp632I* CTCTTC 3029 3032 . . 3040 3043 + SetI ASST 3043 3039 . . 3040 3043 + CviJI RGCY 3041 3041 . . 3040 3043 + AluI AGCT 3041 3041 . . 3046 3050 - TspDTI ATGAA 3036 3034 . . 3052 3055 + AciI CCGC 3052 3054 . . 3055 3059 + DdeI CTNAG 3055 3058 . . 3059 3062 + CviJI RGCY 3060 3060 . . 3054 3060 - Bpu10I CCTNAGC 3055 3058 . . 3073 3077 - BccI CCATC 3067 3068 . . 3087 3092 + ApoI RAATTY 3087 3091 . . 3088 3091 + TspEI AATT 3087 3091 . . 3098 3101 + MseI TTAA 3098 3100 . . 3107 3112 + Hpy178III* TCNNGA 3108 3110 . . 3111 3116 + BsmI GAATGC 3117 3115 . . 3128 3132 + MboII GAAGA 3140 3139 . . 3131 3135 + TfiI GAWTC 3131 3134 . . 3131 3135 + HinfI GANTC 3131 3134 . . 3149 3152 + MaeI CTAG 3149 3151 . . 3155 3159 + TspDTI ATGAA 3170 3168 . . 3157 3161 - Hin4II* CCTTC 3151 3150 . . 3172 3177 - Eco57MI CTGRAG 3157 3155 . . 3172 3177 - Eco57I CTGAAG 3157 3155 . . 3189 3192 + TaiI ACGT 3192 3188 . . 3189 3192 + SetI ASST 3192 3188 . . 3189 3192 + MaeII ACGT 3189 3191 . . 3189 3194 + AflIII ACRYGT 3189 3193 . . 3200 3204 + TspDTI ATGAA 3215 3213 . . 3202 3206 + MboII GAAGA 3214 3213 . . 3237 3243 + TspRI CASTGNN 3243 3234 . . 3236 3240 - BsrI ACTGG 3236 3234 . . 3235 3240 - BfiI ACTGGG 3230 3229 . . 3243 3248 + XhoII RGATCY 3243 3247 . . 3243 3247 + BinI* GGATC 3251 3252 . . 3244 3247 + DpnI GATC 3245 3245 . . 3244 3247 + BstKTI GATC 3246 3244 . . 3244 3247 + MboI GATC 3243 3247 . . 3254 3258 + Tsp45I GTSAC 3253 3258 . . 3254 3258 + MaeIII GTNAC 3253 3258 . . 3264 3268 + MaeIII GTNAC 3263 3268 . . 3270 3274 + HgaI GACGC 3279 3284 . . 3270 3275 + AcyI GRCGYC 3271 3273 . . 3293 3298 + NdeI CATATG 3294 3296 . . 3301 3304 + SetI ASST 3304 3300 . . 3299 3304 - BspMI ACCTGC 3290 3294 . . 3312 3317 + BclI TGATCA 3312 3316 . . 3313 3316 + DpnI GATC 3314 3314 . . 3313 3316 + BstKTI GATC 3315 3313 . . 3313 3316 + MboI GATC 3312 3316 . . 3315 3319 + Hpy188I TCNGA 3317 3316 . . 3318 3323 + BsmI GAATGC 3324 3322 . . 3326 3331 + BsaAI YACGTR 3328 3328 . . 3327 3330 + TaiI ACGT 3330 3326 . . 3327 3330 + SetI ASST 3330 3326 . . 3327 3330 + MaeII ACGT 3327 3329 . . 3331 3335 + MboII GAAGA 3343 3342 . . 3331 3336 - Ksp632I* CTCTTC 3326 3329 . . 3341 3345 + TspDTI ATGAA 3356 3354 . . 3343 3347 + MboII GAAGA 3355 3354 . . 3353 3358 + SmlI CTYRAG 3353 3357 . . 3353 3358 - Bce83I* CTTGAG 3338 3336 . . 3363 3368 + AclI AACGTT 3364 3366 . . 3364 3367 + TaiI ACGT 3367 3363 . . 3364 3367 + SetI ASST 3367 3363 . . 3364 3367 + MaeII ACGT 3364 3366 . . 3372 3376 + DdeI CTNAG 3372 3375 . . 3372 3376 + BspCNI CTCAG 3385 3383 . . 3372 3376 + BseMII CTCAG 3386 3384 . . 3373 3377 + Hpy188I TCNGA 3375 3374 . . 3385 3390 + SmlI CTYRAG 3385 3389 . . 3385 3390 + XhoI CTCGAG 3385 3389 . . 3385 3390 + BmeT110I CYCGRG 3386 3388 . . 3385 3390 + AvaI CYCGRG 3385 3389 . . 3386 3389 + TaqI TCGA 3386 3388 . . 3386 3391 + Hpy178III* TCNNGA 3387 3389 . . 3398 3401 + CviRI* TGCA 3399 3399 . . 3403 3406 + TaqI TCGA 3403 3405 . . 3416 3421 + SfeI* CTRYAG 3416 3420 . . 3440 3444 + BccI CCATC 3448 3449 . . 3449 3453 + TspDTI ATGAA 3464 3462 . . 3459 3465 + TspRI CASTGNN 3465 3456 . . 3460 3464 + Tsp4CI* ACNGT 3462 3461 . . 3461 3466 + SfeI* CTRYAG 3461 3465 . . #--------------------------------------- # # # AciI: # [CCGC] # 25 30 178 378 419 422 # 2022 # # AclI: # [AACGTT] # 110 3364 # # AcyI: # [GRCGYC] # 203 3271 # # AflIII: # [ACRYGT] # 285 3189 # # AhaIII*: # [TTTAAA] # 11 319 346 440 766 1592 # # AjuI: # [GAANNNNNNNTTGG] # 32 1279 2163 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 874 2566 # # AluI: # [AGCT] # 42 71 181 194 216 416 # 433 614 1307 # # AlwNI: # [CAGNNNCTG] # 801 2673 # # ApoI: # [RAATTY] # 11 31 50 73 74 93 # 96 101 128 131 176 201 # 221 387 555 571 575 # # AsuI*: # [GGNCC] # 47 770 2657 # # AvaI: # [CYCGRG] # 89 3385 # # AvaII: # [GGWCC] # 47 770 2657 # # BaeI: # [ACNNNNGTAYC] # 33 973 2468 # # BamHI: # [GGATCC] # 814 2660 # # BarI: # [GAAGNNNNNNTAC] # 32 197 3245 # # BbvI: # [GCAGC] # 147 3327 # # BbvII*: # [GAAGAC] # 1622 1852 # # BccI: # [CCATC] # 26 46 59 289 310 381 # 396 583 604 780 # # Bce83I*: # [CTTGAG] # 136 569 2769 # # BceAI: # [ACGGC] # 1641 1833 # # BciVI: # [GTATCC] # 339 641 2494 # # BclI: # [TGATCA] # 162 468 1026 1818 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 881 2559 # # BetI*: # [WCCGGW] # 481 983 2010 # # BfiI: # [ACTGGG] # 244 3230 # # BglII: # [AGATCT] # 1699 1775 # # BinI*: # [GGATC] # 13 164 176 223 243 371 # 521 1763 # # BisI: # [GCNGC] # 136 3338 # # BlsI: # [GCNGC] # 137 3337 # # BmeT110I: # [CYCGRG] # 88 3386 # # BmgT120I: # [GGNCC] # 47 771 2656 # # Bpu10I: # [CCTNAGC] # 419 3055 # # BsaAI: # [YACGTR] # 146 3328 # # BsaBI: # [GATNNNNATC] # 968 2506 # # BseBI: # [CCWGG] # 30 76 302 553 1118 1395 # # BseGI: # [GGATG] # 102 444 462 575 792 1099 # # BseMII: # [CTCAG] # 75 88 528 1219 1564 # # BseRI: # [GAGGAG] # 582 1271 1621 # # BsiI*: # [CACGAG] # 580 2894 # # BsiYI*: # [CCNNNNNNNGG] # 5 265 282 309 517 626 # 640 830 # # BslFI: # [GGGAC] # 166 849 883 1576 # # BsmAI: # [GTCTC] # 358 449 2667 # # BsmI: # [GAATGC] # 150 207 1221 1896 # # BspCNI: # [CTCAG] # 75 89 526 1218 1566 # # BspMI: # [ACCTGC] # 184 654 2636 # # BsrBI: # [CCGCTC] # 839 2635 # # BsrDI: # [GCAATG] # 50 1024 2400 # # BsrI: # [ACTGG] # 238 1309 1927 # # BssKI: # [CCNGG] # 30 74 302 553 1120 1395 # # BstKTI: # [GATC] # 5 15 69 72 159 163 # 177 187 189 197 243 471 # 501 1026 # # BstXI: # [CCANNNNNNTGG] # 57 3417 # # BtrI: # [CACGTC] # 1104 2370 # # ClaI: # [ATCGAT] # 269 3205 # # Csp6I: # [GTAC] # 16 30 42 318 622 646 # 789 1011 # # CspCI: # [CAANNNNNGTGG] # 15 15 20 1083 2341 # # CviAII: # [CATG] # 7 40 82 96 101 129 # 166 173 369 425 530 579 # 777 # # CviJI: # [RGCY] # 5 19 36 37 42 71 # 104 107 119 144 158 216 # 245 309 380 414 454 614 # # CviRI*: # [TGCA] # 13 75 137 276 321 458 # 620 654 920 # # DdeI: # [CTNAG] # 15 40 65 75 102 188 # 317 370 1150 1152 # # DpnI: # [GATC] # 5 15 69 72 160 163 # 177 187 189 197 243 470 # 501 1026 # # DsaI*: # [CCRYGG] # 128 579 1355 1412 # # EciI: # [GGCGGA] # 626 2848 # # Eco57I: # [CTGAAG] # 317 589 745 1823 # # Eco57MI: # [CTGRAG] # 198 317 589 592 745 1033 # # EcoNI: # [CCTNNNNNAGG] # 829 1097 1548 # # EcoP15I: # [CAGCAG] # 619 2855 # # EcoRI: # [GAATTC] # 473 900 962 1139 # # EcoRII: # [CCWGG] # 30 74 302 553 1120 1395 # # EcoRV: # [GATATC] # 531 2943 # # FalI: # [AAGNNNNNCTT] # 5 32 3437 # # FatI: # [CATG] # 7 40 82 96 101 128 # 166 173 369 425 530 579 # 778 # # FnuDII*: # [CGCG] # 403 3071 # # FokI: # [GGATG] # 102 442 451 595 785 1099 # # GlaI: # [GCGC] # 80 324 3070 # # GsuI: # [CTGGAG] # 592 943 1939 # # HaeIII: # [GGCC] # 109 3365 # # HgaI: # [GACGC] # 195 3279 # # HhaI: # [GCGC] # 81 324 3069 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 12 32 # 32 32 32 32 32 154 # 257 476 483 531 1369 # # Hin4II*: # [CCTTC] # 27 80 144 196 323 421 # 542 1741 # # Hin6I: # [GCGC] # 79 324 3071 # # HindIII: # [AAGCTT] # 729 2745 # # HinfI: # [GANTC] # 17 41 51 57 58 70 # 81 102 138 156 166 192 # 206 267 343 403 493 633 # # HpaII: # [CCGG] # 482 983 2009 # # HphI: # [GGTGA] # 335 434 1296 1409 # # Hpy166II: # [GTNNAC] # 100 215 257 315 400 557 # 613 1017 # # Hpy178III*: # [TCNNGA] # 18 78 87 108 129 131 # 136 151 159 183 232 239 # 251 279 321 972 # # Hpy188I: # [TCNGA] # 8 47 53 58 91 99 # 189 317 378 552 641 1041 # # Hpy99I: # [CGWCG] # 443 1110 1921 # # Ksp632I*: # [CTCTTC] # 69 89 148 297 520 2351 # # MaeI: # [CTAG] # 260 292 325 352 1012 1233 # # MaeII: # [ACGT] # 37 110 138 265 450 666 # 820 988 # # MaeIII: # [GTNAC] # 10 92 139 149 211 381 # 614 629 1249 # # MboI: # [GATC] # 5 15 69 72 162 163 # 177 187 189 197 243 468 # 501 1026 # # MboII: # [GAAGA] # 3 8 11 12 13 69 # 74 78 87 93 94 119 # 129 202 228 403 409 437 # 490 515 # # MfeI: # [CAATTG] # 616 2858 # # MlyI: # [GAGTC] # 36 81 123 171 174 451 # 510 836 1092 # # MmeI: # [TCCRAC] # 712 786 934 1042 # # MnlI: # [CCTC] # 3 13 27 30 40 41 # 90 90 107 119 209 213 # 358 376 379 492 887 # # MseI: # [TTAA] # 6 11 27 37 40 52 # 60 66 77 78 84 92 # 96 108 126 129 145 150 # 189 196 218 257 376 412 # 442 # # MslI: # [CAYNNNNRTG] # 55 72 188 515 590 1025 # 1029 # # NcoI: # [CCATGG] # 128 579 1355 1412 # # NdeI: # [CATATG] # 180 3294 # # NlaIII: # [CATG] # 7 40 82 96 101 132 # 166 173 369 425 530 579 # 774 # # NlaIV: # [GGNNCC] # 72 225 812 2365 # # PfoI: # [TCCNGGA] # 959 2515 # # PleI: # [GAGTC] # 37 81 124 170 173 450 # 510 837 1092 # # PsiI: # [TTATAA] # 110 691 1151 1522 # # PsrI: # [GAACNNNNNNTAC] # 29 32 3413 # # RsaI: # [GTAC] # 17 30 42 318 622 646 # 788 1011 # # SapI: # [GCTCTTC] # 514 2960 # # ScrFI: # [CCNGG] # 30 76 302 553 1118 1395 # # SecI*: # [CCNNGG] # 45 54 74 118 233 301 # 1294 1355 # # SetI: # [ASST] # 11 12 15 15 16 19 # 22 23 26 28 31 32 # 33 37 42 46 48 48 # 69 71 72 95 98 106 # 107 112 122 133 135 147 # 149 149 151 159 181 194 # 215 240 265 # # SfaNI: # [GCATC] # 171 621 2682 # # SfeI*: # [CTRYAG] # 13 45 154 225 1198 1839 # # SmlI: # [CTYRAG] # 32 89 605 2748 # # SpeI: # [ACTAGT] # 291 3183 # # SplI*: # [CGTACG] # 790 2684 # # SspI: # [AATATT] # 327 329 356 597 1865 # # StuI: # [AGGCCT] # 109 3365 # # StyI: # [CCWWGG] # 45 128 534 1355 1412 # # TaiI: # [ACGT] # 37 107 138 265 450 666 # 820 991 # # TaqI: # [TCGA] # 17 71 269 360 660 834 # 1263 # # TatI: # [WGTACW] # 15 1616 1843 # # TfiI: # [GAWTC] # 58 70 166 223 343 403 # 464 468 633 646 # # TseI: # [GCWGC] # 135 3339 # # TsoI: # [TARCCA] # 250 656 708 751 1109 # # Tsp45I: # [GTSAC] # 221 622 2631 # # Tsp4CI*: # [ACNGT] # 12 13 29 74 85 86 # 134 134 143 237 250 264 # 267 367 430 472 477 # # TspDTI: # [ATGAA] # 10 11 15 28 33 45 # 107 108 123 141 195 286 # 297 323 521 598 633 # # TspEI: # [AATT] # 11 11 14 14 16 18 # 18 19 21 21 23 25 # 25 29 29 29 31 36 # 38 47 53 54 55 55 # 70 85 87 90 91 93 # 96 100 113 160 168 195 # 200 201 268 378 387 # # TspGWI: # [ACGGA] # 1666 1808 # # TspRI: # [CASTGNN] # 9 222 461 839 1943 # # TstI: # [CACNNNNNNTCC] # 32 1022 2420 # # VspI: # [ATTAAT] # 652 2822 # # XhoI: # [CTCGAG] # 89 3385 # # XhoII: # [RGATCY] # 20 163 177 231 243 885 # 1755 # # XmnI: # [GAANNNNTTC] # 1688 1786 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3474 # Reported_sequences: 1 # Reported_hitcount: 692 #---------------------------------------