Restriction Fragments for UTP30/YKR060W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

UTP30/YKR060W on chromosome XI from coordinates 556518 to 557342.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.32023.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 11 + PleI GAGTC 15 16 . . 7 11 + MlyI GAGTC 16 16 . . 7 11 + HinfI GANTC 7 10 . . 20 25 + PsiI TTATAA 22 22 . . 32 35 + CviJI RGCY 33 33 . . 33 38 + Cac8I GCNNGC 35 35 . . 37 47 + MwoI GCNNNNNNNGC 43 40 . . 46 56 + MwoI GCNNNNNNNGC 52 49 . . 54 57 + CviJI RGCY 55 55 . . 60 63 + TspEI AATT 59 63 . . 73 77 + MboII GAAGA 85 84 . . 73 78 - Ksp632I* CTCTTC 68 71 . . 82 86 + BccI CCATC 90 91 . . 76 86 - Hin4I GAYNNNNNVTC 99 94 67 62 87 92 + AhaIII* TTTAAA 89 89 . . 88 91 + MseI TTAA 88 90 . . 149 153 + BslFI GGGAC 163 167 . . 156 160 - FokI GGATG 142 146 . . 156 160 - BseGI GGATG 155 153 . . 175 178 + MseI TTAA 175 177 . . 182 187 + TatI WGTACW 182 186 . . 183 186 + Csp6I GTAC 183 185 . . 183 186 + RsaI GTAC 184 184 . . 188 191 + SetI ASST 191 187 . . 188 191 + CviJI RGCY 189 189 . . 188 191 + AluI AGCT 189 189 . . 193 198 + AhaIII* TTTAAA 195 195 . . 194 197 + MseI TTAA 194 196 . . 199 204 + StyI CCWWGG 199 203 . . 199 204 + SecI* CCNNGG 199 203 . . 203 209 + PpuMI RGGWCCY 204 207 . . 203 209 + DraII RGGNCCY 204 207 . . 203 207 + BslFI GGGAC 217 221 . . 203 208 + NlaIV GGNNCC 205 205 . . 204 208 + BmgT120I GGNCC 205 206 . . 204 208 + AvaII GGWCC 204 207 . . 204 208 + AsuI* GGNCC 204 207 . . 206 209 + SetI ASST 209 205 . . 227 232 + StyI CCWWGG 227 231 . . 227 232 + SecI* CCNNGG 227 231 . . 231 236 + XhoII RGATCY 231 235 . . 231 236 + NlaIV GGNNCC 233 233 . . 231 236 + BamHI GGATCC 231 235 . . 231 235 + BinI* GGATC 239 240 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 236 239 + MnlI CCTC 246 245 . . 232 236 - BinI* GGATC 226 227 . . 277 280 + TaiI ACGT 280 276 . . 277 280 + SetI ASST 280 276 . . 277 280 + MaeII ACGT 277 279 . . 278 282 + Hpy99I CGWCG 282 277 . . 280 284 + Hpy188I TCNGA 282 281 . . 271 281 - Hin4I GAYNNNNNVTC 294 289 262 257 284 287 + TspEI AATT 283 287 . . 279 284 - MmeI TCCRAC 260 258 . . 297 300 + TspEI AATT 296 300 . . 299 304 + PsiI TTATAA 301 301 . . 308 313 + Hpy178III* TCNNGA 309 311 . . 315 320 + SmlI CTYRAG 315 319 . . 315 320 + Bce83I* CTTGAG 336 334 . . 321 324 + TaiI ACGT 324 320 . . 321 324 + SetI ASST 324 320 . . 321 324 + MaeII ACGT 321 323 . . 318 322 - BsmAI GTCTC 312 316 . . 318 323 - Esp3I CGTCTC 312 316 . . 326 330 + TfiI GAWTC 326 329 . . 326 330 + HinfI GANTC 326 329 . . 338 343 + Hpy166II GTNNAC 340 340 . . 354 359 + PsiI TTATAA 356 356 . . 367 375 + Tth111I GACNNNGTC 370 371 . . 391 396 + Hpy166II GTNNAC 393 393 . . 393 397 - HphI GGTGA 385 384 . . 405 410 + Hpy178III* TCNNGA 406 408 . . 405 410 + BetI* WCCGGW 405 409 . . 405 410 + BspMII* TCCGGA 405 409 . . 406 409 + HpaII CCGG 406 408 . . 410 413 + SetI ASST 413 409 . . 409 412 - MnlI CCTC 402 401 . . 434 438 + Tsp4CI* ACNGT 436 435 . . 446 449 + TspEI AATT 445 449 . . 456 459 + FatI CATG 455 459 . . 456 459 + NlaIII CATG 459 455 . . 456 459 + CviAII CATG 456 458 . . 459 462 + DpnI GATC 460 460 . . 459 462 + BstKTI GATC 461 459 . . 459 462 + MboI GATC 458 462 . . 462 465 + AciI CCGC 462 464 . . 459 463 - BinI* GGATC 453 454 . . 464 469 + NspI RCATGY 468 464 . . 465 468 + FatI CATG 464 468 . . 465 468 + NlaIII CATG 468 464 . . 465 468 + CviAII CATG 465 467 . . 469 472 + TaqI TCGA 469 471 . . 482 485 + TspEI AATT 481 485 . . 491 496 + Hpy166II GTNNAC 493 493 . . 491 496 + HindII GTYRAC 493 493 . . 489 493 - HgaI GACGC 478 483 . . 503 506 + TaqI TCGA 503 505 . . 512 516 + Tsp45I GTSAC 511 516 . . 512 516 + MaeIII GTNAC 511 516 . . 519 528 + MslI CAYNNNNRTG 523 523 . . 523 528 + BsaAI YACGTR 525 525 . . 524 527 + TaiI ACGT 527 523 . . 524 527 + SetI ASST 527 523 . . 524 527 + MaeII ACGT 524 526 . . 530 535 + SduI GDGCHC 534 530 . . 530 535 + HgiAI* GWGCWC 534 530 . . 535 540 + MfeI CAATTG 535 539 . . 536 539 + TspEI AATT 535 539 . . 559 562 + SetI ASST 562 558 . . 560 563 + MnlI CCTC 570 569 . . 588 593 + SmlI CTYRAG 588 592 . . 588 593 + AflII CTTAAG 588 592 . . 589 592 + MseI TTAA 589 591 . . 593 597 - HgaI GACGC 582 587 . . 632 637 + ApoI RAATTY 632 636 . . 633 636 + TspEI AATT 632 636 . . 638 641 + CviRI* TGCA 639 639 . . 645 649 - FokI GGATG 631 635 . . 645 649 - BseGI GGATG 644 642 . . 669 675 + TspRI CASTGNN 675 666 . . 684 688 - BsmAI GTCTC 678 682 . . 688 698 + BsiYI* CCNNNNNNNGG 694 691 . . 688 691 + AciI CCGC 688 690 . . 684 689 - Eco31I GGTCTC 678 682 . . 695 699 + TauI GCSGC 698 695 . . 695 699 + BlsI GCNGC 697 696 . . 695 699 + BisI GCNGC 696 697 . . 697 702 + AcyI GRCGYC 698 700 . . 695 698 - AciI CCGC 695 697 . . 698 702 - HgaI GACGC 687 692 . . 704 707 + MseI TTAA 704 706 . . 710 713 - MnlI CCTC 703 702 . . 731 736 + Hpy178III* TCNNGA 732 734 . . 740 744 + MaeIII GTNAC 739 744 . . 737 741 - BsrI ACTGG 737 735 . . 743 747 + Tsp4CI* ACNGT 745 744 . . 745 750 + TatI WGTACW 745 749 . . 745 750 + ScaI AGTACT 747 747 . . 746 749 + Csp6I GTAC 746 748 . . 746 749 + RsaI GTAC 747 747 . . 748 753 + SpeI ACTAGT 748 752 . . 749 752 + MaeI CTAG 749 751 . . 772 776 + Hpy188I TCNGA 774 773 . . 777 780 + CviJI RGCY 778 778 . . 775 778 - MnlI CCTC 768 767 . . 779 782 + MaeI CTAG 779 781 . . 789 793 + Hpy188I TCNGA 791 790 . . 797 801 + TspDTI ATGAA 812 810 . . 799 803 + MboII GAAGA 811 810 . . 801 806 + XhoII RGATCY 801 805 . . 801 806 + BglII AGATCT 801 805 . . 802 805 + DpnI GATC 803 803 . . 802 805 + BstKTI GATC 804 802 . . 802 805 + MboI GATC 801 805 . . 804 808 + Hpy188I TCNGA 806 805 . . 805 809 + DdeI CTNAG 805 808 . . 805 809 - BspCNI CTCAG 797 795 . . 805 809 - BseMII CTCAG 796 794 . . 815 818 + MseI TTAA 815 817 . . 818 821 + TspEI AATT 817 821 . . #--------------------------------------- # # # AciI: # [CCGC] # 7 130 226 462 # # AcyI: # [GRCGYC] # 127 698 # # AflII: # [CTTAAG] # 237 588 # # AhaIII*: # [TTTAAA] # 89 106 630 # # AluI: # [AGCT] # 189 636 # # ApoI: # [RAATTY] # 193 632 # # AsuI*: # [GGNCC] # 204 621 # # AvaII: # [GGWCC] # 204 621 # # BamHI: # [GGATCC] # 231 594 # # BccI: # [CCATC] # 90 735 # # Bce83I*: # [CTTGAG] # 336 489 # # BetI*: # [WCCGGW] # 405 420 # # BglII: # [AGATCT] # 24 801 # # BinI*: # [GGATC] # 13 214 226 372 # # BisI: # [GCNGC] # 129 696 # # BlsI: # [GCNGC] # 128 697 # # BmgT120I: # [GGNCC] # 205 620 # # BsaAI: # [YACGTR] # 300 525 # # BseGI: # [GGATG] # 155 181 489 # # BseMII: # [CTCAG] # 29 796 # # BsiYI*: # [CCNNNNNNNGG] # 131 694 # # BslFI: # [GGGAC] # 54 163 608 # # BsmAI: # [GTCTC] # 147 312 366 # # BspCNI: # [CTCAG] # 28 797 # # BspMII*: # [TCCGGA] # 405 420 # # BsrI: # [ACTGG] # 88 737 # # BstKTI: # [GATC] # 21 227 234 343 # # Cac8I: # [GCNNGC] # 35 790 # # Csp6I: # [GTAC] # 79 183 563 # # CviAII: # [CATG] # 9 360 456 # # CviJI: # [RGCY] # 22 33 47 134 589 # # CviRI*: # [TGCA] # 186 639 # # DdeI: # [CTNAG] # 20 805 # # DpnI: # [GATC] # 22 227 233 343 # # DraII: # [RGGNCCY] # 204 621 # # Eco31I: # [GGTCTC] # 147 678 # # Esp3I: # [CGTCTC] # 312 513 # # FatI: # [CATG] # 9 361 455 # # FokI: # [GGATG] # 142 194 489 # # HgaI: # [GACGC] # 104 105 138 478 # # HgiAI*: # [GWGCWC] # 291 534 # # Hin4I: # [GAYNNNNNVTC] # 32 32 67 163 531 # # HindII: # [GTYRAC] # 332 493 # # HinfI: # [GANTC] # 7 319 499 # # HpaII: # [CCGG] # 406 419 # # HphI: # [GGTGA] # 385 440 # # Hpy166II: # [GTNNAC] # 53 100 332 340 # # Hpy178III*: # [TCNNGA] # 93 97 309 326 # # Hpy188I: # [TCNGA] # 15 17 19 282 492 # # Hpy99I: # [CGWCG] # 282 543 # # Ksp632I*: # [CTCTTC] # 68 757 # # MaeI: # [CTAG] # 30 46 749 # # MaeII: # [ACGT] # 44 203 277 301 # # MaeIII: # [GTNAC] # 86 228 511 # # MboI: # [GATC] # 24 227 231 343 # # MboII: # [GAAGA] # 14 85 726 # # MfeI: # [CAATTG] # 290 535 # # MlyI: # [GAGTC] # 16 809 # # MmeI: # [TCCRAC] # 260 565 # # MnlI: # [CCTC] # 57 65 133 156 168 246 # # MseI: # [TTAA] # 10 19 87 88 111 115 # 395 # # MslI: # [CAYNNNNRTG] # 302 523 # # MwoI: # [GCNNNNNNNGC] # 9 43 773 # # NlaIII: # [CATG] # 9 357 459 # # NlaIV: # [GGNNCC] # 28 205 592 # # NspI: # [RCATGY] # 357 468 # # PleI: # [GAGTC] # 15 810 # # PpuMI: # [RGGWCCY] # 204 621 # # PsiI: # [TTATAA] # 22 55 279 469 # # RsaI: # [GTAC] # 78 184 563 # # ScaI: # [AGTACT] # 78 747 # # SduI: # [GDGCHC] # 291 534 # # SecI*: # [CCNNGG] # 28 199 598 # # SetI: # [ASST] # 18 35 44 71 89 114 # 191 263 # # SmlI: # [CTYRAG] # 237 273 315 # # SpeI: # [ACTAGT] # 77 748 # # StyI: # [CCWWGG] # 28 199 598 # # TaiI: # [ACGT] # 44 203 280 298 # # TaqI: # [TCGA] # 34 322 469 # # TatI: # [WGTACW] # 80 182 563 # # TauI: # [GCSGC] # 127 698 # # TfiI: # [GAWTC] # 326 499 # # Tsp45I: # [GTSAC] # 314 511 # # Tsp4CI*: # [ACNGT] # 80 309 436 # # TspDTI: # [ATGAA] # 13 812 # # TspEI: # [AATT] # 8 13 36 54 59 97 # 149 185 224 # # TspRI: # [CASTGNN] # 150 675 # # Tth111I: # [GACNNNGTC] # 370 455 # # XhoII: # [RGATCY] # 24 231 570 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 825 # Reported_sequences: 1 # Reported_hitcount: 163 #---------------------------------------